CN102669056A - Cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation - Google Patents
Cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation Download PDFInfo
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- CN102669056A CN102669056A CN2011100577212A CN201110057721A CN102669056A CN 102669056 A CN102669056 A CN 102669056A CN 2011100577212 A CN2011100577212 A CN 2011100577212A CN 201110057721 A CN201110057721 A CN 201110057721A CN 102669056 A CN102669056 A CN 102669056A
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Abstract
The invention provides a cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation, named portal vein branch suspension way. The method comprises the steps of: respectively embedding silk yarns to serve as traction lines through initial segments of left and right branches of free portal vein during excision of a receptor liver; after a superior vena cava and an inferior vena cava of the receptor liver are reconstructed, suspending the left and right branch traction lines of the portal vein to the head and the external side of the rat and keeping a certain tensile force to enable the left and right branches of the portal vein to be fixed at suitable positions and angles; shearing off the front wall of the portal vein at a junction part of the left and right branches of the receptor portal vein and trimming the front wall into an enlarged U-shaped opening; embedding a cuff-like vessel of a donor portal vein into a receptor portal vein cavity through the U-shaped opening at the junction part of the branches of the receptor portal vein and bundling the cuff-like vessel annularly by the silk yarns. The cuff-like vessel imbedding method used for the portal vein reconstruction of the rat liver transplantation, disclosed by the invention, has the advantages of being easy, rapid and reliable to operate and being capable of shortening training time and saving cost when the cuff-like vessel imbedding method is used for portal vein reconstruction of the rat liver transplantation.
Description
Technical field
The present invention relates to a kind of portal vein method for reconstructing that is used for Rat Liver Transplantation, relate in particular to a kind of cuff pipe method for posting that the Rat Liver Transplantation portal vein is rebuild that is used for, be suitable in organ transplant animal model structure field, using.
Background technology
Since Kamada equals " office cuff " reconstructing blood vessel technology to be introduced Rat Liver Transplantation in 1979; " two office cuff " becomes the standard art formula of Rat Liver Transplantation model gradually; Be widely used in research fields such as trnasplantion immunity, little graft, ischemical reperfusion injury, become the important tool of organ transplant basic research.
" two office cuff " Rat Liver Transplantation is rapid, the complicated meticulous and challenging surgical procedure of a multistep; Set up stable " two oversleeve " method Rat Liver Transplantation model not a duck soup; Wherein no liver in the phase liver superior and inferior vena cava rebuild on the basis; Portal vein cuff pipe is inserted just becomes Rapid Realization liver resurgent and successful key link and the difficult point of operation, particularly most important to the beginner.
At present; It mainly is Kamada used " portal vein batter seam is pricked suspension method " that portal vein cuff pipe is inserted; And " the micro-closer method " of development of domestic part Study center and application; That is: with micro-closer make supply the acceptor portal vein treat anastomotic part near, be inserted in the cuff pipe with microforceps upset acceptor side door wall of vein, realize that acceptor portal vein cuff pipe inserts.All there are problems in these two kinds of methods; The micro-device method of closing up is high to specification requirement; Put incorrect causing easily such as fixing, the position of micro-closer and treat that the anastomosis of blood vessel both sides are difficult to rebuild near accomplishing; And be prone to come off after being prone to cause trunk of portal vein to tear or insert in the portal vein chamber during wall of vein of upset trunk of portal vein etc., short-term training is difficult for successfully; Portal vein rear wall batter suture suspension method seam is earlier pricked portal vein rear wall two side legs; Suspension traction again; Also cause portal vein cuff pipe to insert the portal vein rear wall batter avulsion that bundle was torn or stitched to trunk wall of vein very thin in the process easily; Need again the suture suspention, cause repeatedly the cuff pipe and insert, prolong no time liver phase, or even cause portal vein to rebuild or operative failure.
Summary of the invention
The objective of the invention is in order to overcome the deficiency of above-mentioned prior art; Provide a kind of cuff that is used for the reconstruction of Rat Liver Transplantation portal vein easy and simple to handle, that success rate is high to manage method for posting, particularly provide a kind of beginner to manage method for posting through the cuff that is used for the reconstruction of Rat Liver Transplantation portal vein that short-term training can reach standardization and sequencing grasp.
The objective of the invention is to realize through following proposal: free portal vein left and right sides branch initial segment during Rat Liver Transplantation acceptor hepatectomy, insert the 5-0 silk thread,, the acceptor portal vein ties a knot after driving blood, and subsequent use as draught line.After acceptor liver superior and inferior vena cava is rebuild; Portal vein left and right sides branch draught line is suspended in midair to rat head, the outside, and keep certain tension force, make to prop up about portal vein to be fixed on correct position and angle; Branch's fluidic junction is cut off acceptor portal vein antetheca in the portal vein left and right sides; And be trimmed to the U type opening of an expansion, and portal vein blocking-up folder is moved to trunk of portal vein splenic vein level, retract the portal vein antetheca; Clamping portal vein cuff pipe shank will supply vena portae hepatica cuff pipe to insert in the portal vein chamber through the pylic U type of acceptor opening, prick fixing with 5-0 silk thread ring.
The present invention is owing to adopt such scheme, thereby has the following advantages: 1, free, the suspention silk thread of branch of portal vein initial segment operation such as purchase all no liver before the phase, so this does not increase no time liver phase; 2, can be earlier during free branch of portal vein initial segment prop up and walk around right of portal vein and stab an aperture, get final product with a little gap of two expansions of microforceps portal vein bifurcated again at crotch with curved microforceps list, safe and effective, time saving and energy saving; 3, knotting in advance after branch of portal vein suspention line presets but do not tighten up neither influences the portal vein puncture and drives blood, again operation is placed on no liver before the phase, reduces the interior operation of no liver phase to greatest extent; 4, the portal vein wall is very thin; Improved " portal vein branch suspension method " realized suspension traction with the silk thread ligation in complete portal vein branch; Really fixing, solid reliable, the caused portal vein wall of method of having avoided seam to prick portal vein rear wall batter tears or draught line comes off; Cause repeatedly the cuff pipe and insert, prolong no time liver phase, or even cause portal vein to rebuild and operative failure; 5, earlier with microforceps in the acceptor portal vein left and right sides fluidic junction antetheca cut off an osculum, cut off the U type that is expanded into again to the left and right respectively, the external caliber that expansion can supply the cuff pipe to insert is beneficial to portal vein and rebuilds.
Use cuff pipe method for posting---" portal vein branch suspension method " that portal vein is rebuild; The beginner can reach standardization and the sequencing that portal vein cuff pipe is inserted technology through short-term training; Accomplish acceptor portal vein cuff pipe fast and insert, realize that portal vein is rebuild in the operation of Rat Liver Transplantation acceptor.
Description of drawings
Fig. 1 is the branch of portal vein suspention line sketch map of purchasing.
Fig. 2 suspends in midair for branch of portal vein and the cuff pipe is inserted sketch map.
Specific embodiments
In order to understand essence of the present invention better; Be provided for a kind of embodiment of the cuff pipe method for posting of " two office cuff " Rat Liver Transplantation portal vein reconstruction below, but these specific embodiments not to limit invention scope required for protection by any way.
1 material: the SD rat, male, body weight 180g-220g, water is can't help in fasting before the art.Each cover of micro-surgical instruments and routine operation apparatus, 5-0 silk thread and 8-0 crosspointer blood vessel NS are some, available from Shanghai Medical Apparatus and Instruments Factory; 2 of self-control abdominal retractors, 1 in self-control open anesthesia tube, self-control infrahepatic vena cava and portal vein cuff pipe are some; Chloraldurate, absolute ether, heparin, atropine are some etc.
2 operation methods:
(1) during Rat Liver Transplantation row acceptor hepatectomy, free receptor portal vein left and right sides branch initial segment is inserted the 5-0 silk thread respectively, knotting but do not tighten up, so far, the completion of purchasing of branch of portal vein suspention line.Get into no liver after date, puncture portal vein crotch injects normal temperature physiological saline 3ml and drives blood.Behind the blocking-up liver superior and inferior vena cava liver superior and inferior vena cava; Liver is turned over to a rat side, ligation, tighten up about portal vein a 5-0 silk thread that presets, suspension traction is used when treating that portal vein cuff pipe is inserted; At ligature distal severed branch of portal vein; So far, branch of portal vein suspention line is purchased and is finished, referring to Fig. 1.
(4) Rat Liver Transplantation supplies in the liver implant surgery, after rebuilding acceptor liver superior and inferior vena cava, will supply liver to turn over cephalad, paves.With two vessel forceps respectively clamp portal vein left and right sides branch draught line suspend in midair to rat head, the outside, keep certain tension force, and fixing suitable angle and position; Earlier with microforceps in the acceptor portal vein left and right sides branch's fluidic junction antetheca cut off an osculum, cut off the U type that is expanded into again to the left and right respectively, enlarge the external caliber that can supply the cuff pipe to insert with this; Portal vein blocking-up folder is moved to trunk of portal vein splenic vein level; Decontrol the blood vessel clip bloodletting slightly to get rid of high blood coagulation, wash down clot and bubble in the portal vein chamber, retract the portal vein antetheca with a microforceps with physiological saline; Another microforceps clamping portal vein cuff manages shank; Portal vein cuff pipe is inserted in the acceptor portal vein chamber through the U type opening of portal vein fluidic junction, prick with 5-0 silk thread ring and fix, so far; Portal vein cuff pipe is inserted technology " portal vein branch suspension method " row Rat Liver Transplantation acceptor portal vein cuff pipe and is inserted and portal vein reconstruction completion, referring to Fig. 2.
The partial reference document that the present invention relates to
[1]Kamada?N，Calne?RY.Orthotopic?liver?transplantation?in?the?rat：Technique?using?cuff?for?portal?vein?anastomosis?and?biliary?drainage.Transplantation，1979，28：47-50.
[2]Kamada?N，Calne?RY.A?surgical?experience?with?five?hundred?thirty?l?iver?transplants?in?the?rat.Surgery，1983，93：64-69.
[3]Delriviere?L，Gibbs?P，Kobayashi?E，et?al.Technical?details?for?safer?venous?and?biliary?anastomoses?for?liver?transplantation?in?the?rat.Microsurgery，1998，18：12-18.
[4] Ma Yi, He Xiaoshun, outmoded conventions are drawn. the operation skill of rat orthotopic liver transplantation model and the prevention of complication. Chinese microsurgery magazine, 2003,26:45-47.
All documents that the present invention mentions are all quoted in application as a reference, are just all quoted such as a reference separately as each piece document.Should understand in addition; The present invention combines most preferred embodiment to describe; Yet after having read foregoing of the present invention, those skilled in the art can do various changes or modification to the present invention, and these equivalent form of values fall within the application's accompanying claims institute restricted portion equally.
Claims (4)
1. one kind is used for cuff pipe method for posting---" portal vein branch suspension method " that the Rat Liver Transplantation portal vein is rebuild, and this method is carried out as follows:
(1) when the acceptor hepatectomy, free portal vein left and right sides branch inserts draught line respectively, after the acceptor portal vein drives blood, ties a knot;
(2) after the liver superior and inferior vena cava is rebuild, with the draught line suspention of acceptor portal vein left and right sides branch;
(3) the U type opening that enlarges is cut off and be trimmed to the portal vein antetheca of acceptor portal vein left and right sides branch fluidic junction;
(4) will supply vena portae hepatica cuff pipe to insert in the acceptor portal vein chamber, prick fixing with the silk thread ring through the U type opening of acceptor portal vein left and right sides branch fluidic junction.
2. " portal vein branch suspension method " as claimed in claim 1 is characterized in that: what when the acceptor hepatectomy, dissociated is acceptor portal vein left and right sides branch initial segment.
3. " portal vein branch suspension method " as claimed in claim 1, it is characterized in that: the draught line of being inserted is the 5-0 silk thread.
4. " portal vein branch suspension method " as claimed in claim 1; It is characterized in that: the direction that portal vein left and right sides branch draught line is suspended in midair after the liver superior and inferior vena cava is rebuild is rat head, the outside; And keep certain tension force; Make to prop up about portal vein to be fixed on correct position and angle, be beneficial to the pruning of the fluidic junction U of portal vein left and right sides branch type opening and inserting of portal vein cuff pipe.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100577212A CN102669056A (en) | 2011-03-10 | 2011-03-10 | Cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation |
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| CN2011100577212A CN102669056A (en) | 2011-03-10 | 2011-03-10 | Cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2807911C1 (en) * | 2023-09-07 | 2023-11-21 | Сергей Эдуардович Восканян | Method for transplantation of right lobe of liver in presence of three branches of portal vein in transplant |
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2011
- 2011-03-10 CN CN2011100577212A patent/CN102669056A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2807911C1 (en) * | 2023-09-07 | 2023-11-21 | Сергей Эдуардович Восканян | Method for transplantation of right lobe of liver in presence of three branches of portal vein in transplant |
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Application publication date: 20120919 |