CN102645361B - Automatic treatment device for animal feed AFB1 content detection sample - Google Patents
Automatic treatment device for animal feed AFB1 content detection sample Download PDFInfo
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- CN102645361B CN102645361B CN 201210121834 CN201210121834A CN102645361B CN 102645361 B CN102645361 B CN 102645361B CN 201210121834 CN201210121834 CN 201210121834 CN 201210121834 A CN201210121834 A CN 201210121834A CN 102645361 B CN102645361 B CN 102645361B
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- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 101100449517 Arabidopsis thaliana GRH1 gene Proteins 0.000 title claims abstract description 24
- 101100434479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) AFB1 gene Proteins 0.000 title claims abstract description 24
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 title claims abstract description 24
- 229930020125 aflatoxin-B1 Natural products 0.000 title claims abstract description 24
- 239000002904 solvent Substances 0.000 claims abstract description 66
- 238000005303 weighing Methods 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 13
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 239000000243 solution Substances 0.000 claims description 62
- 239000003153 chemical reaction reagent Substances 0.000 claims description 32
- 238000004090 dissolution Methods 0.000 claims description 9
- 230000007306 turnover Effects 0.000 claims description 7
- 230000007246 mechanism Effects 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 abstract description 10
- 239000000523 sample Substances 0.000 description 58
- 239000007788 liquid Substances 0.000 description 5
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 5
- 238000005259 measurement Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000007865 diluting Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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Abstract
The invention relates to an automatic treatment device for an animal feed AFB1 content detection sample, which comprises a powder sample weighing device, a solvent measuring device, a mixing dissolver and a detection sample extraction device. The powder sample weighing device comprises a charging barrel, a fixed semicircular sheet, a movable semicircular sheet, a rotary shaft, a motor and an electronic scale, wherein the fixed semicircular sheet and the movable semicircular sheet are arranged at the bottom end of the charging barrel, and the rotary shaft drives the movable semicircular sheet. The solvent measuring device comprises a solvent tank, a controllable flow electromagnetic valve, a solvent measuring vessel, a float sensor I, a solvent guide tube and a switching electromagnetic valve I. The mixing dissolver comprises a triangular flask, an electromagnetic valve and a stirring device which are positioned below the electronic scale and an outlet end of the solvent guide tube. The detection sample extraction device comprises a solution measuring vessel, a float sensor II and a switching electromagnetic valve II, wherein the solution measuring vessel with a filter screen is positioned below the triangular flask, and the switching electromagnetic valve II is connected into a solution delivery tube. Working efficiency and detection accuracy can be effectively improved by using the device for preparing sample extracting solution.
Description
Technical field
The present invention relates to a kind of test sample automatic processing device, especially a kind of animal feed AFB1 content detection sample automatic processing device.
Background technology
AFB1 has induced mutation, Immunosuppression and carcinogenesis, and the serious harm human and animal is healthy, if AFB1 content overproof cause that easily animal is poisoning in animal feed, and accumulate in animal product with residual, affect the animal product quality, then be linked to the mankind, be detrimental to health.Therefore, in animal feed, the measurement of AFB1 content is extremely important.
It is as follows that at present animal feed inspection center carries out animal feed AFB1 content detection sample treatment: get the 5.0g sample in 100ml tool plug triangular flask, accurately add 25.0ml methanol-water (1+1) solution, vibration 15min, filter, discard 1/4 and just collect filtrate after filtrate, this liquid is sample extracting solution.National allowance standard value is per sample suitably diluted sample extracting solution with A reagent (sample diluting liquid), is sample measuring liquid, sample measuring liquid is detected namely obtain feed AFB1 content.
All adopt manual mode of operation and configure in the whole operating process of sample measuring liquid in the above.Adopt manual operations that two large deficiencies are arranged: the one, inefficiency, detect pre-treatment and adopt manual operations, one day about energy 8 parts of test sample (perfect condition), calculated in five days with a week job, approximately can only detect approximately 40 parts of Feed Samples one week, this detection speed is difficult to adapt to the requirement of large-scale industrial production; The 2nd, accuracy of detection is not high, error is large, owing to being manual operations, when very most of weighing and measurement article, what adopt is the range estimation mode, the result that different people's range estimations obtains can be different, can cause personal error, adopts manual accuracy rate of carrying out the pre-treatment test sample to be about 96% according to statistics.
Summary of the invention
The purpose of this invention is to provide a kind of can effectively increasing work efficiency and the animal feed AFB1 content detection sample automatic processing device of accuracy of detection.
the technical scheme that the present invention adopts for achieving the above object is: a kind of animal feed AFB1 content detection sample automatic processing device, and this device comprises the powdered sample weighing device, the solvent dispensing device, the mixed dissolution device, test sample extraction element, powdered sample weighing device comprise the barrel for the dress powdered sample, be arranged on fixedly halfround bar slice and the movable halfround bar slice of barrel bottom, the rotating shaft that is connected with movable halfround bar slice, the motor of drive shaft motion, be arranged on the electronic scales of barrel below, the solvent dispensing device comprises the solvent cell for the dress solvent, control the flow-controllable solenoid valve of solvent streams rate of discharge, the solvent streams that is arranged on solvent cell exports the solvent measuring device of below, be arranged in the solvent measuring device in order to respond to the float sensor I of quantity of solvent, the solvent mozzle that is connected with solvent measuring device bottom, be connected to the switch electromagnetic valve I in the solvent mozzle, the mixed dissolution device comprises the triangular flask that is arranged on electronic scales and solvent draft tube outlets end below, be arranged in the solenoid valve that solution that triangular flask bottom makes triangular flask flows out triangular flask, in order to stir the stirring apparatus of potpourri in triangular flask, the test sample extraction element comprises the solution measuring device that is positioned at the triangular flask below, be positioned at the filter screen of solution measuring device upper end, be arranged in the solution measuring device in order to respond to the float sensor II of mixed solution amount, be positioned at the solution delivery line in the outside, solution measuring device bottom, be connected to the switch electromagnetic valve II in the solution delivery line.
Further technical scheme of the present invention is: the bottom of described barrel is cylindric, and fixedly halfround bar slice is fixedly connected on the outer wall of barrel bottom, movable halfround bar slice under the power of motor with together relative fixedly halfround bar slice rotation of rotating shaft.
Further technical scheme again of the present invention is: the bottom of described electronic scales is provided with panel turnover switch.
Of the present invention more further technical scheme be: described stirring apparatus comprise extend to the paddle in triangular flask and be arranged on triangular flask outer to drive the vibrating mechanism of paddle work.
Further technical scheme of the present invention is: described solution measuring device one side is provided with the reagent container for installed reagents, and reagent container is connected in the solution measuring device by reagent conduit, is provided with the switch electromagnetic valve III of controlling reagent dosage in reagent conduit.
Owing to adopting said structure, the present invention's animal feed AFB1 content detection sample automatic processing device has following beneficial effect:
1, improved work efficiency
the present invention's animal feed AFB1 content detection sample automatic processing device is due to the weight of powdered sample weighing device energy automatic weighing powdered sample, the amount of solvent dispensing device energy automatic measurement solvent, powdered sample and solvent are put in the triangular flask of mixed dissolution device, the stirring apparatus automatic stirring fully dissolves powdered sample, fully the action of dissolving rear electromagnetic valve is put into solution in the solution amount device automatically, and automatic fitration in the process of putting, when needing to add reagent, reagent container adds reagent by the switch electromagnetic valve III in trend solution amount device, addition is controlled automatically by the switch electromagnetic valve III, after preparing sample extracting solution, by gauge tap solenoid valve II, sample extracting solution is put in predetermined container, can detect sample extracting solution, whole process (weigh by powdered sample, solvent measures, mixed dissolution, sample extraction) all take robotization to control, through facts have proved, adopt the work of this device, the energy test sample reached more than 100 parts in one day, calculated in five days with a week job, can detect in one week and surpass 500 parts of Feed Samples, compared with prior art can effectively increase work efficiency,
2, improved accuracy of detection
The present invention's animal feed AFB1 content detection sample automatic processing device is due to the weight of powdered sample weighing device energy automatic weighing powdered sample, the amount of solvent dispensing device energy automatic measurement solvent, effectively avoid because the error that range estimation brings, through facts have proved, adopt the sample extracting solution of this automatic processing device preparation, its AFB1 content detection accuracy rate is 99.9%, therefore adopts automatic processing device of the present invention can effectively improve accuracy of detection.
Below in conjunction with drawings and Examples, animal feed AFB1 content detection sample automatic processing device of the present invention is described further.
Description of drawings
Fig. 1 is animal feed AFB1 content detection sample automatic processing device structural representation of the present invention.
main element label declaration: 1-motor, 2-rotating shaft, 3-barrel, 4-fixedly halfround bar slices, 5-movable halfround bar slice, 6-electronic scales, 7-panel turnover switch, 8-triangular flask, 9-solenoid valve, 10-solution measuring device, 11-float sensor II, 12-solvent cell, 13-flow-controllable solenoid valve, 14-float sensor I, 15-solvent measuring device, 16-switch electromagnetic valve I, 17-solvent mozzle, 18-vibrating mechanism, 19-filter screen, 20-switch electromagnetic valve III, 21-reagent conduit, 22-reagent container, 23-switch electromagnetic valve II, 24-solution delivery line.
Embodiment
As shown in Figure 1, a kind of animal feed AFB1 of the present invention content detection sample automatic processing device, this device comprises powdered sample weighing device, solvent dispensing device, mixed dissolution device, test sample extraction element.
The powdered sample weighing device comprises for the barrel 3 of dress powdered sample, the fixedly halfround bar slice 4 that is arranged on barrel 3 bottoms and the halfround bar slice of be connected 5, the rotating shaft 2 that is connected with movable halfround bar slice 5 upper center, the motor 1 that drive shaft 2 is moved, the electronic scales 6 that is arranged on barrel 3 belows, the bottom of electronic scales 6 is provided with panel turnover switch 7, and panel turnover switch 7 is by the automatically controlled switch of realizing.Barrel 3 is up big and down small cylindric, fixedly halfround bar slice 4 is fixedly connected on the outer wall of barrel 3 bottoms, movable halfround bar slice 5 also is arranged at barrel 3 bottoms, movable halfround bar slice 5 is low or higher than fixing 4 one disk thickness of halfround bar slice at vertical direction, movable halfround bar slice 5 can be under the power of motor 1 and together relative fixedly halfround bar slice 4 rotations of rotating shaft 2, when movable halfround bar slice 5 rotates to when overlapping with fixed wafer, the aperture of barrel 3 is maximum, when movable halfround bar slice 5 rotates to the position relative with fixed wafer, the sealing of the bottom of barrel 3.Certainly, movable halfround bar slice 5 also is not limited to rotate, movable halfround bar slice 5 also relatively fixedly halfround bar slice 4 vertical directions move up and down, at this moment, the difference in height that movable halfround bar slice 5 moves up or down is larger, the aperture of barrel 3 is larger, and movable halfround bar slice 5 and fixed wafer flush when relative barrel 3 bottoms and seal.
The solvent dispensing device comprise for the solvent cell 12 of dress solvent, control the solvent streams rate of discharge flow-controllable solenoid valve 13, be arranged on the solvent streams outlet below of solvent cell 12 solvent measuring device 15, be arranged on solvent measuring device 15 in order to the float sensor I 14 of responding to quantity of solvent, the solvent mozzle 17 that is connected with solvent measuring device 15 bottoms, be connected to the switch electromagnetic valve I 16 in solvent mozzle 17.Flow-controllable solenoid valve 13 is arranged on a side in the solvent streams outlet of solvent cell 12.
The mixed dissolution device comprises the triangular flask 8 that is arranged on electronic scales 6 and solvent mozzle 17 endpiece belows, be arranged in the triangular flask bottom make the solution of triangular flask 8 flow out the solenoid valve 9 of triangular flask 8, in order to stirring the stirring apparatus of triangular flask 8 potpourris, stirring apparatus comprise extend to the paddle in triangular flask 8 and be arranged on triangular flask 8 outer to drive the vibrating mechanism 18 of paddle work.
The test sample extraction element comprises the solution measuring device 10, the filter screen 19 that is arranged in solution measuring device 10 upper ends that are arranged in triangular flask 8 belows, is arranged on solution measuring device 10 in order to float sensor II 11, the solution delivery line 24 that is arranged in solution measuring device 10 outsides, bottoms of responding to solution measuring device 10 mixed solution amounts, the switch electromagnetic valve II 23 that is connected to solution delivery line 24.Described solution measuring device 10 1 sides are provided with the reagent container 22 for installed reagents, and reagent container 22 is connected in solution measuring device 10 by reagent conduit 21, are provided with the switch electromagnetic valve III 20 of controlling reagent dosage in reagent conduit 21.
The work of automatic processing device of the present invention is controlled by the PLC control system, the drive motor of motor 1, electronic scales 6, panel turnover switch 7, flow-controllable solenoid valve 13, float sensor I 14, switch electromagnetic valve I 16, solenoid valve 9, vibrating mechanism 18 or motor, float sensor II 11, switch electromagnetic valve II 23, switch electromagnetic valve III 20 etc. all are connected (the PLC control system is not that inventive point of the present invention is not described in detail its structure at this) by circuit with the PLC control system, by the unified control of PLC control system automatic processing device work of the present invention.
during work, at first, by powdered sample weighing device weighing 5g powdered sample, relatively fixedly halfround bar slice 4 rotations of movable halfround bar slice 5 under the drive of motor 1, the openings of sizes of two halfround bar slices can determine the speed that powder feed falls, powder feed begins greatly, and speed falls, electronic scale claims to get the general assembly (TW) of whereabouts powder feed, when the electronic scale perception will reach 5g, the PLC control system is controlled two halfround bar slice openings of sizes and is diminished, make the last feed whereabouts rate reduction (assurance precision) of powder falling, until make the general assembly (TW) of feed whereabouts, powder falling end reach 5g, at this moment two halfround bar slice openings are closed fully, secondly, measure 25.0ml methanol-water (1+1) solution (solvent that namely is used for the dissolved powders feed) by the solvent dispensing device, methanol-water (1+1) solution is housed in solvent cell 12, begin to make flow-controllable solenoid valve 13 to inject methanol-water (1+1) solution with maximum flow to solvent measuring device 15, signal with float sensor I 14 in solvent measuring device 15, more near 25ml, control flow-controllable solenoid valve 13 flows less, until reach 25ml, flow-controllable solenoid valve 13 cuts out fully, the 3rd, panel turnover switch 7 upsets of control electronic scales 6 bottoms make the 5g powder feed of weighing pour in triangular flask 8, gauge tap solenoid valve I 16 simultaneously, 25ml methanol-water (1+1) solution is all flow in triangular flask 8, then, drive paddle work by vibrating mechanism 18, (the paddle vibration is 15min approximately) controls solenoid valve 9 unlatchings of triangular flask 8 bottoms after powder feed fully dissolves, solution flows to solution amount device 10, solution before flowing to solution measuring device 10 by being positioned at filter screen 19 filtering solutions of solution measuring device 10 upper ends, float sensor II 11 is arranged in solution measuring device 10, the total amount of solution can be detected, put 1/4 solution by switch electromagnetic valve II 23, then open in reagent container 22(reagent container 22 A reagent be housed) and the switch electromagnetic valve III 20 of 10, solution measuring device, inject A reagent in solution measuring device 10, delay time and treat after 5 minutes that sample extracting solution obtains suitably diluting and namely obtain sample extracting solution, sample extracting solution is emitted and can be checked by switch electromagnetic valve II 23.The process for preparation of whole sample extracting solution (powdered sample is weighed, solvent measures, mixed dissolution, sample extraction) all takes robotization to control, and can effectively increase work efficiency and accuracy of detection.
As variation of the present invention, the present invention is not limited to the sample extracting solution of preparing animal fodder AFB1 content detection sample, also can be used for preparing the sample extracting solution of other test sample; If powdered sample is very easy to be dissolved in solvent, also stirring apparatus can be set; When sample extracting solution need not to add reagent, also can not be provided for adding reagent container, reagent conduit, the switch electromagnetic valve III of reagent; As long as the conversion of doing within the scope of the invention all belongs to category of the present invention.
Claims (5)
1. an animal feed AFB1 content detection sample automatic processing device, is characterized in that, this device comprises the powdered sample weighing device, the solvent dispensing device, the mixed dissolution device, test sample extraction element, powdered sample weighing device comprise the barrel (3) for the dress powdered sample, be arranged on fixedly halfround bar slice (4) and the movable halfround bar slice (5) of barrel (3) bottom, the rotating shaft (2) that is connected with movable halfround bar slice (5), the motor (1) of drive shaft (2) motion, be arranged on the electronic scales (6) of barrel (3) below, the solvent dispensing device comprises the solvent cell (12) for the dress solvent, control the flow-controllable solenoid valve (13) of solvent streams rate of discharge, the solvent streams that is arranged on solvent cell (12) exports the solvent measuring device (15) of below, be arranged in solvent measuring device (15) in order to respond to the float sensor I (14) of quantity of solvent, the solvent mozzle (17) that is connected with solvent measuring device (15) bottom, be connected to the switch electromagnetic valve I (16) in solvent mozzle (17), the mixed dissolution device comprises the triangular flask (8) that is arranged on electronic scales (6) and solvent mozzle (17) endpiece below, be arranged in the solenoid valve (9) that solution that triangular flask (8) bottom makes triangular flask (8) flows out triangular flask (8), in order to stir the stirring apparatus of potpourri in triangular flask (8), the test sample extraction element comprises the solution measuring device (10) that is positioned at triangular flask (8) below, be positioned at the filter screen (19) of solution measuring device (10) upper end, be arranged in solution measuring device (10) in order to respond to the float sensor II (11) of mixed solution amount, be positioned at the solution delivery line (24) in the outside, solution measuring device (10) bottom, be connected to the switch electromagnetic valve II (23) in solution delivery line (24).
2. a kind of animal feed AFB1 content detection sample automatic processing device as claimed in claim 1, it is characterized in that, the bottom of described barrel (3) is cylindric, fixedly halfround bar slice (4) is fixedly connected on the outer wall of barrel (3) bottom, movable halfround bar slice (5) under the power of motor (1) with together relative fixedly halfround bar slice (4) rotation of rotating shaft (2).
3. a kind of animal feed AFB1 content detection sample automatic processing device as claimed in claim 1, is characterized in that, the bottom of described electronic scales (6) is provided with panel turnover switch (7).
4. a kind of animal feed AFB1 content detection sample automatic processing device as claimed in claim 1, it is characterized in that, described stirring apparatus comprise extend to the paddle in triangular flask (8) and be arranged on triangular flask (8) outer to drive the vibrating mechanism (18) of paddle work.
5. a kind of animal feed AFB1 content detection sample automatic processing device as claimed in claim 1, it is characterized in that, described solution measuring device (10) one sides are provided with the reagent container (22) for installed reagents, reagent container (22) is connected in solution measuring device (10) by reagent conduit (21), is provided with the switch electromagnetic valve III (20) of controlling reagent dosage in reagent conduit (21).
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| CN 201210121834 CN102645361B (en) | 2012-04-24 | 2012-04-24 | Automatic treatment device for animal feed AFB1 content detection sample |
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| CN103604676A (en) * | 2013-12-02 | 2014-02-26 | 中山鼎晟生物科技有限公司 | Agricultural product detected sample collecting device |
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| CN112683639B (en) * | 2020-12-22 | 2021-11-26 | 安徽合大环境检测有限公司 | A quartering ware for solid sample handles |
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| JP3027799B2 (en) * | 1996-02-19 | 2000-04-04 | 栗田工業株式会社 | Sampling device |
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Application publication date: 20120822 Assignee: ADANA FOOD TECH (LIUZHOU) Co.,Ltd. Assignor: LIUZHOU VOCATIONAL & TECHNICAL College Contract record no.: X2023980053965 Denomination of invention: An automatic sample processing device for detecting AFB1 content in animal feed Granted publication date: 20131106 License type: Common License Record date: 20231225 |