CN102643167A - Alga endophytic fungus sesquiterpene compound and preparation method and application thereof - Google Patents
Alga endophytic fungus sesquiterpene compound and preparation method and application thereof Download PDFInfo
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- 241000233866 Fungi Species 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 229930004725 sesquiterpene Natural products 0.000 title abstract description 6
- -1 sesquiterpene compound Chemical class 0.000 title abstract description 6
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- 238000000855 fermentation Methods 0.000 claims abstract description 9
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- 238000000926 separation method Methods 0.000 claims description 3
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- RIYUSAKJWZXQID-BYNSBNAKSA-N C[C@](CO)(CCC1)[C@H](CC2)[C@@]1(C)[C@@H](CO)C2=C Chemical compound C[C@](CO)(CCC1)[C@H](CC2)[C@@]1(C)[C@@H](CO)C2=C RIYUSAKJWZXQID-BYNSBNAKSA-N 0.000 description 1
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Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The invention relates to the field of insecticides and bacterial inhibitors, in particular to an alga endophytic fungus sesquiterpene compound and a preparation method and application thereof. The specific structural formula is shown as a formula (I). The preparation method of the alga endophytic fungus sesquiterpene compound comprises the steps of inoculating aspergillus versicolor DL-29 in a fungus liquid culture medium for fermentation and culture and purifying fermentation products to obtain the alga endophytic fungus sesquiterpene compound shown as the formula (I). According to insecticidal activity experiments, the obtained alga endophytic fungus sesquiterpene compound has the advantages that the fatality rate of artemia is 73.8 percent when the concentration of the compound is 100 micrograms per milliliter, the lethal concentration is 35.0 micrograms per milliliter when the fatality rate of artemia is 50 percent, and the bacteriostatic activity is remarkable.
Description
Technical field
The present invention relates to sterilant and bacterial inhibitor field, specifically a kind of marine alga endogenetic fungus sesquiterpenoids and preparation and application.
Background technology
Along with the chemical synthetic pesticide passing of duration of service, the detrimentally affect of its generation is more and more outstanding." green barrier " that causes because of chemical pesticide is residual seriously restricting the outlet of agricultural products in China, and the traditional agriculture industry is faced with bigger risk and challenge.Compare with traditional chemical synthetic pesticide, biogenic pesticide has people and animals and non-target organism safety, and environment compatibility is good, is difficult for producing advantages such as resistance.
Pathogenic colon bacillus causes that through polluting drinking-water, food etc. outbreak of disease is popular, and the person of being in a bad way can critical life, and Chang Zuowei drinks water and the hygiology standard of food (or medicine); Streptococcus aureus is human a kind of important pathogen, can cause multiple severe infections.Because the variance ratio of mikrobe is very fast, constantly develop immunity to drugs etc., the development of new bacterial inhibitor is still very urgent.
Summary of the invention
The purpose of this invention is to provide a kind of marine alga endogenetic fungus sesquiterpenoids and preparation and application.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is:
A kind of marine alga endogenetic fungus sesquiterpenoids, marine alga endogenetic fungus sesquiterpenoids is suc as formula shown in (I)
The preparation method of marine alga endogenetic fungus sesquiterpenoids; DL-29 is inoculated in fermentation culture in the fungi liquid substratum with aspergillus versicolor (Aspergillus versicolor), behind the tunning purifying, is the marine alga endogenetic fungus sesquiterpenoids shown in the formula (I); Said aspergillus versicolor (Aspergillus versicolor) DL-29; It is stored in Chinese typical culture collection center C CTCC on November 25th, 2011, and deposit number is CCTCC M 2011421
Concrete preparation process:
1) aspergillus versicolor (Aspergillus versicolor) DL-29 was inoculated in the fungi liquid substratum static fermentation 30 days; Filter; Fermented liquid concentrates through ethyl acetate extraction, and the mycelium of collection is used organic solvent extraction, then concentrates through ethyl acetate extraction again; Fermented liquid gained enriched material and mycelium gained enriched material merge, and are crude extract; Said aspergillus versicolor (Aspergillus versicolor) DL-29, it is stored in Chinese typical culture collection center C CTCC on November 25th, 2011, and deposit number is CCTCC M 2011421;
2) crude extract of getting in the step 1) carries out silica gel column chromatography, carries out gradient elution with organic solvent, collects elutriant, and elutriant detects through thin-layer chromatography;
3) collect step 2) in effluent volume than 2-3: the elution fraction of 1 gradient; The elution fraction of collecting is carried out gel filtration chromatography, performance liquid and thin-layer chromatography separation and purification; It is the 0.3-0.4 component that purifying is collected the Rf value, promptly gets suc as formula the marine alga endogenetic fungus sesquiterpenoids shown in (I).
Organic solvent extraction liquid in the step 1) be in chloroform, acetone, ETHYLE ACETATE, ethanol or the methyl alcohol one or more.Step 2) organic solvent in is petroleum ether-ethyl acetate or sherwood oil-acetone.The said gel filtration chromatography elutriant of step 3) is volume ratio 1-2: 1 chloroform-methanol or chloroform-ethanol; The eluent of performance liquid is volume ratio 4-6: 1 methanol-water; The thin-layer chromatography developping agent is that volume ratio is 1-2: 1 petroleum ether-ethyl acetate or sherwood oil-acetone.Collect the component of orange spot during the said gel filtration chromatography of step 3).
The application of marine alga endogenetic fungus sesquiterpenoids, said marine alga endogenetic fungus sesquiterpenoids is used to prepare desinsection or inhibiting-bacteria preparation.Said marine alga endogenetic fungus sesquiterpenoids is used to prepare the pesticide preparation of halogen worm.Said marine alga endogenetic fungus sesquiterpenoids is used to prepare intestinal bacteria or staphylococcic biocide preparation.
The present invention has the following advantages: the marine alga endogenetic fungus sesquiterpenoids that the present invention obtains through extraction, separation through fungi aspergillus versicolor (Aspergillus versicolor) the DL-29 fermentation that is located away from the marine green algae Codiumfragile(sur.) Hariot.; Lethality rate to the halogen worm when the insecticidal activity experiment draws compound in 100 mcg/ml is 73.8%, and median lethal concentration is 35.0 mcg/ml; Draw compound through the bacteriostatic activity experiment and have tangible bacteriostatic activity.
Embodiment:
Below in conjunction with embodiment the present invention is done further elaboration.
Embodiment 1
Marine alga endogenetic fungus sesquiterpenoids is suc as formula shown in (I).
Embodiment 2
Preparing method suc as formula the marine alga endogenetic fungus sesquiterpenoids shown in (I):
Well-grown fungi aspergillus versicolor (Aspergillus versicolor) the DL-29 bacterial classification on the plate of making even; Being cut into small pieces is inoculated in the PDB liquid nutrient medium, puts the 300mL substratum in every 1L triangular flask, totally 50 bottles; The static fermentation of room temperature 30 days; The ETHYLE ACETATE that adds fermented liquid 1/2nd volumes is killed fungi, filters, and collects mycelium and fermented liquid respectively.Said PDB liquid nutrient medium consists of every liter and contains 200 milliliters of murphy juices, glucose 20 grams, peptone 5 grams, yeast extract paste 3 grams, 500 milliliters of Chen Haishui, 300 milliliters of zero(ppm) water.Its; Fungi aspergillus versicolor (Aspergillus versicolor) DL-29 bacterial classification on November 25th, 2011 was stored in Chinese typical culture collection center C CTCC; Deposit number is CCTCC M 2011421, classification name: Aspergillus versicolor, and strain number is DL-29;
Collect the about 15L of fermented liquid, with ethyl acetate extraction three times, concentrating under reduced pressure; Mycelium is pulverized the back with 1: 1 chloroform-methanol extraction of volume ratio three times, uses ethyl acetate extraction again, concentrating under reduced pressure; It is similar that enriched material detects its result through thin-layer chromatography, and merging fermented liquid and the two-part enriched material of mycelium is crude extract 29.4g.Crude extract is carried out 100-200 purpose silica gel column chromatography, with petroleum ether-ethyl acetate with 100: 0,50: 1; 20: 1,10: 1,5: 1; 2: 1 to 0: 100 gradient is carried out wash-out, collects elutriant respectively, uses thin-layer chromatography (TLC) to detect (when thin-layer chromatography detects with aubepine-sulfuric acid as developer) again; Judge, merge identical or similar portions according to the Rf value, obtain 14 components (1-14).Component 9 is promptly carried out gel column, performance liquid and thin-layer chromatography with the component under 2: 1 gradient elutions of petroleum ether-ethyl acetate again separate, elutriant is with 1: 1 chloroform-methanol of volume ratio during gel filtration chromatography, and TLC detects; Collect the component of sorrel spot; The performance liquid elutriant is 85: 15 a methanol-water of volume ratio, and thin-layer chromatography is a developping agent with 1: 1 petroleum ether-ethyl acetate of volume ratio, and collecting the Rf value is the component of 0.3-0.4; Get compound (3.8 milligrams) shown in the formula (I); Detect through TLC, be single, even orange spot, confirm as pure compound.Through Spectrum Analysis, its structure is accredited as a kind of new sesquiterpene, structural formula shown in (I), called after Albican-11,14-diol.
This compound has following physics and chemistry and spectral characteristic:
Colorless oil, specific rotatory power [α]
17 D:-15.0 (c 0.15, CHCl
3); Proton nmr spectra (1H-NMR, CDCl
3, 500MHz) δ
H1.28 (m), 1.72 (m), 1.52 (m), 1.52 (m), 1.01 (m), 1.85 (m), 1.31 (m), 1.36 (m); 1.85 (m), 2.01 (m), 2.44 (m), 1.99 (m), 3.76 (dd, 10.9,9.7); (3.84 dd, 10.9,3.5), 4.65 (d, 1.2), 4.94 (d, 1.2); 0.70 (s), 3.41 (dd, 10.8,0.8), 3.74 (d, 10.8), 0.99 (s); Carbon-13 nmr spectra (
13C-NMR, CDCl
3, 125MHz) δ
C38.9CH
2, 18.8CH
2, 35.3CH
2, 38.9C, 56.0CH, 24.2CH
2, 38.2CH
2, 147.4C, 59.2CH, 38.8C, 58.8CH
2, 106.6CH
2, 16.1CH
3, 65.1CH
2, 27.1CH
3High resolution mass spectrum (HREIMS) [M]
+M/z 238.1925, calculated value 238.1933.
Embodiment 3
Be with embodiment 2 differences
Well-grown fungi aspergillus versicolor (Aspergillus versicolor) the DL-29 bacterial classification on the plate of making even; Being cut into small pieces is inoculated in the PDB liquid nutrient medium, puts the 300mL substratum in every 1L triangular flask, totally 50 bottles; The static fermentation of room temperature 30 days; Add 1/2nd volume ETHYLE ACETATE and kill fungi, filter, collect mycelium and fermented liquid respectively.Said PDB liquid nutrient medium consists of every liter and contains 200 milliliters of murphy juices, glucose 20 grams, peptone 5 grams, yeast extract paste 3 grams, 500 milliliters of Chen Haishui, 300 milliliters of zero(ppm) water.
Collect the about 15L of fermented liquid, with ethyl acetate extraction three times, concentrating under reduced pressure; Mycelium is pulverized the back with acetone extraction three times, uses ethyl acetate extraction again, concentrating under reduced pressure; It is similar that enriched material detects its result through thin-layer chromatography, and merging fermented liquid and the two-part enriched material of mycelium is crude extract 28.0g.Crude extract is carried out 100-200 purpose silica gel column chromatography, with sherwood oil-acetone with 100: 0,50: 1; 30: 1,20: 1,15: 1; 7: 1,3: 1 to 0: 100 gradient was carried out wash-out, collected elutriant respectively; Use thin-layer chromatography (TLC) to detect (when thin-layer chromatography detects with aubepine-sulfuric acid as developer) again, judge, merge identical or similar portions, obtain 14 components (1-14) according to the Rf value.With component 9 is that 3: 1 components under the gradient elution are carried out gel column, performance liquid and thin-layer chromatography again and separated with sherwood oil-acetone volume ratio promptly; Elutriant is chloroform-ethanol of 2: 1 of volume ratio during gel filtration chromatography, and TLC detects, and collects the component of orange spot; The performance liquid elutriant is 80: 20 a methanol-water of volume ratio; Thin-layer chromatography is a developping agent with 1: 1 petroleum ether-ethyl acetate, and collecting the Rf value is the component of 0.3-0.4, gets the sesquiterpenoids shown in the formula (I).
Embodiment 4
The insecticidal activity experiment:
The halogen worm (Brine Shrimp) also claim the salt solution fairy shrimp, belongs to Arthropoda in the classification, Crustachia, and Anostraca, salt solution fairy shrimp section, genus artemia, the halogen worm receives people's extensive attention as a kind of important and good laboratory animal material always.Adopt this insect that cultivate in the laboratory under the control condition, borrow it to come the power of assessing compound insecticidal activity.
The hatching of artemia cysts: get artemia cysts and place 500 ml beakers for 100 milligrams, add 400 milliliters of artificial seawaters, slowly inflate with a little aerator pump, chorion and unhatched ovum are removed in room temperature hatching 24 hours, and halogen worm larva continues to cultivate 24 hours, and is subsequent use.
The biological method that causes death of halogen worm: according to the improved method of Solis, get 96 porocyte culture plates, every hole adds the artificial seawater liquid that 190 microlitres contain 10 left and right sides halogen worm larvas, processes the test cultures plate.The sample sets of blank group and each concentration is respectively established three parallel holes; The blank group adds 10 microlitre solvent DMSO 99.8MIN.s (DMSO); Sample sets adds the solution (DMSO is a solvent, and concentration is 2.0 mg/ml) of the sesquiterpenoids shown in the 10 microlitre formulas (I).After the incubated at room temperature 24 hours, under the binocular anatomical lens, detect the dead individual number of counting halogen worm.
The biological activity that causes death of halogen worm representes that with corrected mortality calculation formula is following:
Corrected mortality=(control group survival rate-treatment group survival rate)/control group survival rate * 100%
Experimental result: the diterpene alkaloid compounds of the acquisition in the foregoing description lethality rate to the halogen worm when 100 mcg/ml is 73.8%, and median lethal concentration is 35.0 mcg/ml.
Embodiment 5
The bacteriostatic activity experiment:
ETEC (Escherichia coli) is commonly referred to intestinal bacteria; Be the Gram-negative tyrothricin, pathogenic colon bacillus causes that outbreak of disease is popular, the person of being in a bad way through polluting drinking-water, food etc.; Can critical life, the hygiology standard of Chang Zuowei drinking-water and food (or medicine); Streptococcus aureus (Staphyloccocus aureus) is human a kind of important pathogen; Be gram-positive microorganism, be under the jurisdiction of Staphylococcus, can cause multiple severe infections; They also are experimental strains commonly used in the laboratory, so borrow it to come assessing compound to suppress the power of bacterial activity.
Be specially: testing used bacteria culture medium is the LB substratum, leaches bacteria suspension with aseptic cotton carrier, evenly is applied on the substratum; Specimen is dissolved among the DMSO; Concentration is 6.0 mg/ml, and getting 5 microlitre samples, to be added to diameter be (every 30 microgram) on 5 millimeters the aseptic filter paper sheet, dries; And with being added with equal volume DMSO and the air dried filter paper is done negative control, as antibacterial positive control, each three parallel with paraxin.The plate culture medium that is added with sample places 37 ℃ to leave standstill and cultivated 24 hours, and its antibacterial circle diameter of measurement of inhibition zone appears in the observation experiment result.
Experimental result: the sesquiterpenoids of above-mentioned acquisition is respectively 7.0 millimeters and 10.3 millimeters to the antibacterial circle diameter of intestinal bacteria and streptococcus aureus, has the activity that suppresses intestinal bacteria and streptococcus aureus.
Claims (10)
1. marine alga endogenetic fungus sesquiterpenoids, it is characterized in that: marine alga endogenetic fungus sesquiterpenoids is suc as formula shown in (I)
2. the preparation method of the described marine alga endogenetic fungus of claim 1 sesquiterpenoids; It is characterized in that: DL-29 is inoculated in fermentation culture in the fungi liquid substratum with aspergillus versicolor (Aspergillus versicolor); Behind the tunning purifying; Be the marine alga endogenetic fungus sesquiterpenoids shown in the formula (I), said aspergillus versicolor (Aspergillus versicolor) DL-29, it is stored in Chinese typical culture collection center C CTCC on November 25th, 2011; Deposit number is CCTCC M 2011421
3. by the preparation method of the described marine alga endogenetic fungus of claim 2 sesquiterpenoids, it is characterized in that concrete preparation process:
1) aspergillus versicolor (Aspergillus versicolor) DL-29 was inoculated in the fungi liquid substratum static fermentation 30 days; Filter; Fermented liquid concentrates through ethyl acetate extraction, and the mycelium of collection is used organic solvent extraction, then concentrates through ethyl acetate extraction again; Fermented liquid gained enriched material and mycelium gained enriched material merge, and are crude extract;
Said aspergillus versicolor (Aspergillus versicolor) DL-29, it is stored in Chinese typical culture collection center C CTCC on November 25th, 2011, and deposit number is CCTCC M 2011421;
2) crude extract of getting in the step 1) carries out silica gel column chromatography, carries out gradient elution with organic solvent, collects elutriant, and elutriant detects through thin-layer chromatography;
3) collect step 2) in effluent volume than 2-3: the elution fraction of 1 gradient; The elution fraction of collecting is carried out gel filtration chromatography, performance liquid and thin-layer chromatography separation and purification; It is the 0.3-0.4 component that purifying is collected the Rf value, promptly gets suc as formula the marine alga endogenetic fungus sesquiterpenoids shown in (I).
4. by the preparation method of the described marine alga endogenetic fungus of claim 3 sesquiterpenoids, it is characterized in that: the organic solvent extraction liquid in the step 1) be in chloroform, acetone, ETHYLE ACETATE, ethanol or the methyl alcohol one or more.
5. by the preparation method of the described marine alga endogenetic fungus of claim 3 sesquiterpenoids, it is characterized in that: step 2) in organic solvent be petroleum ether-ethyl acetate or sherwood oil-acetone.
6. by the preparation method of the described marine alga endogenetic fungus of claim 3 sesquiterpenoids, it is characterized in that: the said gel filtration chromatography elutriant of step 3) is volume ratio 1-2: 1 chloroform-methanol or chloroform-ethanol; The eluent of performance liquid is volume ratio 4-6: 1 methanol-water; The thin-layer chromatography developping agent is that volume ratio is 1-2: 1 petroleum ether-ethyl acetate or sherwood oil-acetone.
7. by the preparation method of the described marine alga endogenetic fungus of claim 3 sesquiterpenoids, it is characterized in that: the component of collecting the orange spot during the said gel filtration chromatography of step 3).
8. the application of the described marine alga endogenetic fungus of claim 1 sesquiterpenoids is characterized in that: said marine alga endogenetic fungus sesquiterpenoids is used to prepare desinsection or inhibiting-bacteria preparation.
9. by the application of the described marine alga endogenetic fungus of claim 8 sesquiterpenoids, it is characterized in that: said marine alga endogenetic fungus sesquiterpenoids is used to prepare the pesticide preparation of halogen worm.
10. by the application of the described marine alga endogenetic fungus of claim 8 sesquiterpenoids, it is characterized in that: said marine alga endogenetic fungus sesquiterpenoids is used to prepare intestinal bacteria or staphylococcic biocide preparation.
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