CN102634514A - Bay scallop heat-resistance-related metallothionein gene marker and assisted breeding method thereof - Google Patents
Bay scallop heat-resistance-related metallothionein gene marker and assisted breeding method thereof Download PDFInfo
- Publication number
- CN102634514A CN102634514A CN2012100397394A CN201210039739A CN102634514A CN 102634514 A CN102634514 A CN 102634514A CN 2012100397394 A CN2012100397394 A CN 2012100397394A CN 201210039739 A CN201210039739 A CN 201210039739A CN 102634514 A CN102634514 A CN 102634514A
- Authority
- CN
- China
- Prior art keywords
- heat resistanceheat
- metallothionein gene
- resistanceheat resistant
- heat
- bay scallop
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of aquatics, and particularly relates to a bay scallop heat-resistance-related metallothionein gene marker and a assisted breeding method thereof. The bay scallop heat-resistance-related metallothionein gene marker is shown in a base sequence of a sequence list SEQ ID No.1, and the upstream 337th basic group A related to an initiator is a heat-resisting gene locus. Polymorphic sites of the bay scallop metallothionein gene are screened initially, the heat resistance related metallothionein gene marker is developed, and the heat resistance gene marker assisted breeding method is established. The bay scallop heat-resistance-related metallothionein gene marker and the heat resistance gene marker assisted breeding method have the advantages of high pertinence, high breeding efficiency, simplicity, convenience and quickness in operation and the like, and are applicable to screening shellfish heat-resistance-related markers and breeding improved heat-resisting breeds.
Description
Technical field
The invention belongs to the aquatic living things technical field, a kind of specifically bay scallop heat resistanceheat resistant associated metal metallothionein gene mark and auxiliary breeding means thereof.
Background technology
The scallop culture of China industry has exposed many problem demanding prompt solutions gradually through after initial period flourish.Secular close relative breeds, breed has for many generations caused inbreeding depression, makes bay scallop poor growth, resistance reduce.Environment in recent years worsens especially that summer high temperature makes the scallop organism metabolic disorder, and extensive death incident often takes place, and causes the tremendous economic loss.Therefore, accelerate the research of breeding core cutting edge technology and the seed selection of degeneration-resistant improved seeds and become the key that ensures the sustainable development of scallop culture industry.But it is because less to the research of shellfish heat resistanceheat resistant mechanism and heat resistanceheat resistant functional gene both at home and abroad at present; Lack the relevant molecule marker of heat resistanceheat resistant; Cause the progress of shellfish heat resistanceheat resistant breed of variety slow, restricted to a great extent that scallop culture is already stable, health and Sustainable development.
RhMT is one type of lower molecular weight, be rich in sulfydryl and protein that can chelated metal ions; In body, exercise multiple function; For example participate in the adjusting of essential metal element in the organism and the detoxification of nonessential metallic element, biochemical reactions such as the removing of active oxygen and body growth, growth, reproduction, aging, tumour generation, immunity, stress response.There are some researches show that some genetic expressions were raised when organism received heat stress, wherein rhMT is particularly violent as the component reaction of redox system.Research shows that rhMT can extensively be removed and comprises superoxide anion, hydrogen peroxide, hydroxyl (ROS class material HO); It is a kind of endogenous inhibitor; It can further regulate and control the expression of other antioxidases simultaneously, thereby assists body antagonism oxidative stress.Research about the shellfish rhMT at present focuses mostly at it to aspects such as heavy metal regulation and control and oxygen scavenging activities; But the relation research between its polymorphum and the shellfish heat hardiness power does not appear in the newspapers yet; Therefore seek the polymorphum of scallop metallothionein gene; And the distribution characteristics of research polymorphic site in heat-resisting relevant different population, will and utilize metallothionein gene to lay the foundation for research as the candidate gene of potential heat impedance marker assisted selection.
Summary of the invention
The objective of the invention is to a kind of bay scallop heat resistanceheat resistant associated metal metallothionein gene mark and auxiliary breeding means thereof.
For realizing above-mentioned purpose, the present invention adopts technical scheme to be:
A kind of bay scallop heat resistanceheat resistant associated metal metallothionein gene mark, gulf scallop heat resistanceheat resistant associated metal metallothionein gene is labeled as shown in the sequence table SEQ ID No.1 base sequence, and the 337th base A of upstream from start codon is the heat resistanceheat resistant gene locus.
The acquisition of bay scallop heat resistanceheat resistant associated metal metallothionein gene mark obtains as follows:
1) clone of bay scallop metallothionein gene promoter region partial sequence; Total DNA with bay scallop closed shell flesh is a template, obtains dna sequence dna according to known metallothionein gene sequence at promoter region design primer AiMT1pF and AiMT1pR amplification, and is for use; AiMT1pF 5 '-atgtaagggcacttatgcttgatctttagt-3 ' and AiMT1pR 5 '-agcagtcaggaccagcacagcca-3 ';
The screening of 2) heat resistanceheat resistant associated metal metallothionein gene mark; Respectively 12 clones of 6 individuals to from heat resistanceheat resistant colony and thermo-responsive colony check order, and its pleomorphism site is-488C/T-450A/C ,-447G/C ,-440A ins-del;-431T/C ,-430G/A ,-428A/T ,-375T/C;-363T/G ,-337A/C ,-311T/A ,-310A/T;-298G/T ,-265A/G ,-253A/G ,-251T/C and-118C/T; After extracting the individual genomic dna of 50 thermo-responsive individualities and 50 heat resistanceheat resistants again, to-the 375T/C site is polymorphic, pcr amplification metallothionein promoter region sequence at first; Select for use Mse I that the PCR product is carried out enzyme then and cut, behind the polyacrylamide gel electrophoresis ,-375T/T;-375T/C and-3 kinds of genotype of 375C/C, with-the 337A/C site is polymorphic, utilizes Bi-PASA PCR to analyze; Behind the agarose gel electrophoresis;-337A/A ,-337A/C and-3 kinds of genotype of 337C/C analyze through Chi-square Test; The frequency that-337A/A individuality occurs in heat resistanceheat resistant colony is significantly higher than responsive colony, promptly-and 337A/A is the relevant metallothionein gene mark of heat resistanceheat resistant.
Bay scallop heat resistanceheat resistant associated metal metallothionein gene marker-assisted breeding method; With the metallothionein gene type that occurs in heat resistanceheat resistant colony medium-high frequency as heat resistanceheat resistant associated metal metallothionein gene mark; The bay scallop that carries this heat resistanceheat resistant genes involved mark is bred; Cultivate the offspring, the clone obtains the metallothionein gene promoter region sequence among the offspring, studies its polymorphum; Carry out heat stress simultaneously and handle experiment; The genetic development of research heat resistanceheat resistant associated metal metallothionein gene mark and with the relation of bay scallop temperature capacity; Therefrom filter out and both had heat resistanceheat resistant associated metal metallothionein gene mark, the spat that temperature capacity significantly improves again carry out many generation breedings and cultivate after can set up the heat resistanceheat resistant new variety.
The present invention compared with present technology; The present invention is by Protocols in Molecular Biology; With the bay scallop is that material has been excavated China's cultivated shellfish heat resistanceheat resistant associated metal metallothionein gene mark first; Tentatively set up bay scallop heat resistanceheat resistant associated metal metallothionein gene marker-assisted breeding technology; Characteristics such as that this technology has is simple and efficient to handle, seed selection efficient high, the cycle is short for new molecular breeding technological approaches has been opened up in the cultivation of shellfish heat resistanceheat resistant kind, have most important theories meaning and using value to the seed selection of cultivated shellfish heat resistanceheat resistant kind.
Description of drawings
The bay scallop metallothionein gene promoter region partial sequence figure that Fig. 1 provides for the embodiment of the invention.
The bay scallop metallothionein gene promoter region pleomorphism site sequence chart that Fig. 2 provides for the embodiment of the invention.
((A) Mse I restriction enzyme mapping wherein: M1 is DL2000 Marker to the somatotype collection of illustrative plates of the bay scallop metallothionein gene promoter region different genotype that Fig. 3 provides for the embodiment of the invention; 1 is-375T/T genotype restriction enzyme mapping; 2 are-375T/C genotype restriction enzyme mapping; 3 are-375C/C genotype restriction enzyme mapping, and M2 is 20bp Marker.(B) Bi-PASA PCR collection of illustrative plates: M1 is DL2000 Marker, and 1 is-337A/C genotype PCR collection of illustrative plates, and 2 are-337C/C genotype PCR collection of illustrative plates, and 3 are-337A/A genotype PCR collection of illustrative plates.)。
The distribution frequency of rhMT different genotype and corresponding chi square test figure in bay scallop heat resistanceheat resistant colony that Fig. 4 provides for the embodiment of the invention and the responsive colony.
Embodiment
With the example that is established as of relevant bay scallop metallothionein gene mark of bay scallop heat resistanceheat resistant and assistant breeding technology thereof, technology contents of the present invention is elaborated below.
Bay scallop heat resistanceheat resistant associated metal metallothionein gene mark comprises: 1. the clone of bay scallop metallothionein gene promoter region partial sequence; 2. the screening of heat resistanceheat resistant associated metal metallothionein gene mark; 3. carry the rapid screening of heat resistanceheat resistant genes involved tagging;
1. the clone of bay scallop metallothionein gene promoter region
From bay scallop closed shell flesh, extract total DNA with reference to the said method of molecular cloning; At its promoter region design primer, the clone obtains the dna sequence dna of one section 617 base according to known metallothionein gene sequence, checks order after being connected into the T carrier, obtains its nucleotide sequence.
2. the screening of heat resistanceheat resistant associated metal metallothionein gene mark
Respectively 12 clones of 6 individuals to from heat resistanceheat resistant colony and thermo-responsive colony check order, and have found 17 place's pleomorphism sites; 50 thermo-responsive individualities of picked at random and 50 heat resistanceheat resistant scallops are individual, extract genomic dna, to-the 375T/C site is polymorphic; Pcr amplification metallothionein promoter region sequence at first; Select for use Mse I that the PCR product is carried out enzyme then and cut, behind the polyacrylamide gel electrophoresis, find 3 kinds of genotype;-375T/T ,-375T/C and-375C/C.To-the 337A/C site is polymorphic, utilizes Bi-PASA PCR to analyze, and behind the agarose gel electrophoresis, finds 3 kinds of genotype ,-337A/A ,-337A/C and-337C/C.The frequency that 3 kinds of genotype individualities in-375 sites occur in heat resistanceheat resistant colony and thermo-responsive colony does not have marked difference; And-frequency that the 337A/A individuality occurs in heat resistanceheat resistant colony is significantly higher than thermo-responsive colony, and-frequency that the 337C/C individuality occurs in thermo-responsive colony is significantly higher than heat resistanceheat resistant colony.Therefore, with-337C/C is as thermo-responsive relevant metallothionein gene mark, and general-337A/A is as the relevant metallothionein gene mark of heat resistanceheat resistant.
3. carry the rapid screening of heat resistanceheat resistant genes involved tagging
Get the individual whole blood 1 μ l of bay scallop as masterplate, utilize and analyze with Bi-PASA PCR, behind the agarose gel electrophoresis somatotype, selection-337A/A individuality is individual as heat resistanceheat resistant.
The acquisition of bay scallop heat resistanceheat resistant associated metal metallothionein gene mark obtains as follows:
1. the clone of bay scallop metallothionein gene promoter region
With reference to the said method of molecular cloning, from bay scallop closed shell flesh, extract total DNA; In promoter region design primer AiMT1pF (5 '-atgtaagggcacttatgcttgatctttagt-3 ') and AiMT1pR (5 '-agcagtcaggaccagcacagcca-3 '), carry out pcr amplification according to following program: 94 ℃ of sex change 5min according to known metallothionein gene sequence; Carry out 35 constant amplifications of round-robin (72 ℃ are extended 1min for 94 ℃ of sex change 30s, 55 ℃ of annealing 30s) afterwards; At last at 72 ℃ of insulation 10min.The clone obtains the dna sequence dna of one section 617 base, checks order after being connected into the T carrier, obtains its nucleotide sequence (referring to Fig. 1).Clone's MT promoter sequence total length has 617bp, and according to the prediction of NNPP DB, this fragment has four possible transcription initiation sites (TSS1-TSS4), several core promoter elements TATA box and CAAT box.Patch software and the prediction of TESS database analysis, this sequence contains many potential transcription factor binding site points, comprises metal response element (MREs), glucocorticoid response element (GREs), anti-oxidant response element (AREs), heat shock element (HSE) and SP1.
2. the screening of bay scallop heat resistanceheat resistant associated metal metallothionein gene mark
Respectively 12 clones of 6 individuals to from heat resistanceheat resistant colony and thermo-responsive colony check order, and have found 17 place's pleomorphism sites (488C/T ,-450A/C ,-447G/C ,-440A ins-del;-431T/C ,-430G/A ,-428A/T ,-375T/C;-363T/G ,-337A/C ,-311T/A ,-310A/T;-298G/T ,-265A/G ,-253A/G ,-251T/C with-118C/T) (referring to Fig. 2).After extracting the individual genomic dna of 50 thermo-responsive individualities and 50 heat resistanceheat resistants, to-the 375T/C site is polymorphic, pcr amplification metallothionein promoter region sequence at first, and program is following: 94 ℃ of sex change 5min; Then 35 constant amplifications of round-robin (72 ℃ are extended 1min for 94 ℃ of sex change 30s, 55 ℃ of annealing 30s); At last at 72 ℃ of insulation 10min.Selecting for use Mse I that the PCR product is carried out enzyme then cuts.For-375T allelotrope, can produce size after Mse I enzyme is cut and be respectively 30,54,55,61,65,118,122 with 8 fragments of 407bp, then can produce size and be respectively 30,54 for-375C allelotrope, 55,65,118,122 with 7 fragments of 468bp.Get enzyme and cut product 10 μ L, add tetrabromophenol sulfonphthalein sample-loading buffer 2 μ L, with 180V voltage electrophoresis 120min in 12% polyacrylamide gel.With behind the 0.5 μ g/mL ethidium bromide staining under uv lamp observation whether have 468 to carry out somatotype with two bands of 407bp, find 3 kinds of genotype altogether ,-375T/T ,-375T/C and-375C/C.To-the 337A/C site is polymorphic; With AiMT1pP (5 '-ttcaggttggaaagttcatgtaagggcact-3 '); AiMT1pQ (5 '-caacacaccttcggaagacatcacagc-3 '); AiMT1pA (5 '-ggggggggggcatattttgaacagttc-3 ') and AiMT1pB (5 '-ggggggggggctcagctagacatgat-3 ') be primer, utilize Bi-PASA PCR to analyze.The PCR reaction adopts warm start and landing procedure to come the specificity of enhanced amplification, and reaction conditions is following: 94 ℃ of sex change 5min; Carry out 10 round-robin landing procedures (94 ℃ of sex change 30s, the renaturation temperature is since 63 ℃, whenever carries out a thermal cycling temperature and reduces by 0.8 ℃, lands vertically to 55 ℃, the sex change time is 30s, 72 ℃ are extended 30s) afterwards; Meet 25 the constant amplification of round-robin (94 ℃ of sex change 30s then; 55 ℃ of annealing 30s, 72 ℃ are extended 30s); Last 72 ℃ of insulation 10min.For-337A allelotrope, Bi-PASA PCR can produce size be 226 with 2 fragments of 559bp, for-337C allelotrope then can produce size be respectively 384 with 2 fragments of 559bp.Get PCR product 5 μ L, add tetrabromophenol sulfonphthalein sample-loading buffer 1 μ L, with 120V voltage electrophoresis 30min in 2% sepharose.With under uv lamp, observing banding pattern behind the 0.5 μ g/mL ethidium bromide staining, finds 3 kinds of genotype ,-337A/A ,-337A/C and-337C/C (referring to Fig. 3).
Site-375 and the individual frequency (referring to table 1 and Fig. 4) that occurs of-337 different genotype in statistics heat resistanceheat resistant colony and the susceptible colony; Utilize SPSS11.5 software to carry out Chi-square Test and analyze the back discovery; The frequency that-375 different genotype individualities occur in heat resistanceheat resistant colony and thermo-responsive colony does not have significant difference; And-frequency that the 337C/C individuality occurs in responsive colony is significantly higher than heat resistanceheat resistant colony, and the frequency that-337A/A individuality occurs in heat resistanceheat resistant colony is significantly higher than responsive colony (referring to table 1 and Fig. 4).Therefore ,-337C/C is thermo-responsive relevant metallothionein gene mark, and-337A/A is to be the relevant metallothionein gene mark of heat resistanceheat resistant.
Table 1: the chi square test of bay scallop rhMT different genotype distribution frequency in heat resistanceheat resistant colony and thermo-responsive colony
3. carry the rapid screening of heat resistanceheat resistant genes involved tagging
Get bay scallop whole blood 1 μ l as masterplate; With AiMT1pP (5 '-ttcaggttggaaagttcatgtaagggcact-3 '); AiMT1pQ (5 '-caacacaccttcggaagacatcacagc-3 '), AiMT1pA (5 '-ggggggggggcatattttgaacagttc-3 ') and AiMT1pB (5 '-ggggggggggctcagctagacatgat-3 ') carry out Bi-PASA PCR for primer.The PCR reaction adopts warm start and landing procedure to come the specificity of enhanced amplification, and reaction conditions is following: 94 ℃ of sex change 5min; Carry out 10 round-robin landing procedures (94 ℃ of sex change 30s, the renaturation temperature is since 63 ℃, whenever carries out a thermal cycling temperature and reduces by 0.8 ℃, lands vertically to 55 ℃, the sex change time is 30s, 72 ℃ are extended 30s) afterwards; Meet 25 the constant amplification of round-robin (94 ℃ of sex change 30s then; 55 ℃ of annealing 30s, 72 ℃ are extended 30s); Last 72 ℃ of insulation 10min.Selection-337A/A individuality behind the PCR product agarose gel electrophoresis somatotype is individual as heat resistanceheat resistant.
Bay scallop heat resistanceheat resistant associated metal metallothionein gene marker-assisted breeding method:
With metallothionein gene type-337A/A of occurring in heat resistanceheat resistant colony medium-high frequency as heat resistanceheat resistant associated metal metallothionein gene mark.The bay scallop that carries this heat resistanceheat resistant genes involved mark is bred, cultivate the offspring, the clone obtains the metallothionein gene among the offspring, studies its polymorphum; Carry out heat stress simultaneously and handle experiment; The genetic development of research heat resistanceheat resistant associated metal metallothionein gene mark and with the relation of scallop heat hardiness; Therefrom filter out and both contained heat resistanceheat resistant associated metal metallothionein gene mark; The spat that heat resistance improves again carries out can setting up the heat resistanceheat resistant improved seeds after many generation breedings and the cultivation.
Claims (3)
1. bay scallop heat resistanceheat resistant associated metal metallothionein gene mark; It is characterized in that: bay scallop heat resistanceheat resistant associated metal metallothionein gene is labeled as shown in the sequence table SEQ ID No.1 base sequence, and the 337th base A of upstream from start codon is the heat resistanceheat resistant gene locus.
2. by the said bay scallop heat resistanceheat resistant of claim 1 associated metal metallothionein gene mark, it is characterized in that: the acquisition of bay scallop heat resistanceheat resistant associated metal metallothionein gene mark obtains as follows:
1) clone of bay scallop metallothionein gene promoter region partial sequence; Total DNA with bay scallop closed shell flesh is a template, obtains dna sequence dna according to known metallothionein gene sequence at promoter region design primer AiMT1pF and AiMT1pR amplification, and is for use; AiMT1pF 5 '-atgtaagggcacttatgcttgatctttagt-3 ' and AiMT1pR 5 '-agcagtcaggaccagcacagcca-3 ';
The screening of 2) heat resistanceheat resistant associated metal metallothionein gene mark; Respectively 12 clones of 6 individuals to from heat resistanceheat resistant colony and thermo-responsive colony check order, and its pleomorphism site is-488C/T-450A/C ,-447G/C ,-440A ins-del;-431T/C ,-430G/A ,-428A/T ,-375T/C;-363T/G ,-337A/C ,-311T/A ,-310A/T;-298G/T ,-265A/G ,-253A/G ,-251T/C and-118C/T; After extracting the individual genomic dna of 50 thermo-responsive individualities and 50 heat resistanceheat resistants again, to-the 375T/C site is polymorphic, pcr amplification metallothionein promoter region sequence at first; Select for use Mse I that the PCR product is carried out enzyme then and cut, behind the polyacrylamide gel electrophoresis ,-375T/T;-375T/C and-3 kinds of genotype of 375C/C, with-the 337A/C site is polymorphic, utilizes Bi-PASA PCR to analyze; Behind the agarose gel electrophoresis;-337A/A ,-337A/C and-3 kinds of genotype of 337C/C analyze through Chi-square Test; The frequency that-337A/A individuality occurs in heat resistanceheat resistant colony is significantly higher than responsive colony, promptly-and 337A/A is the relevant metallothionein gene mark of heat resistanceheat resistant.
3. bay scallop heat resistanceheat resistant associated metal metallothionein gene marker-assisted breeding method; It is characterized in that: with the metallothionein gene type that occurs in heat resistanceheat resistant colony medium-high frequency as heat resistanceheat resistant associated metal metallothionein gene mark; The bay scallop that carries this heat resistanceheat resistant genes involved mark is bred; Cultivate the offspring, the clone obtains the metallothionein gene promoter region sequence among the offspring, studies its polymorphum; Carry out heat stress simultaneously and handle experiment; The genetic development of research heat resistanceheat resistant associated metal metallothionein gene mark and with the relation of bay scallop temperature capacity; Therefrom filter out and both had heat resistanceheat resistant associated metal metallothionein gene mark, the spat that temperature capacity significantly improves again carry out many generation breedings and cultivate after can set up the heat resistanceheat resistant new variety.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100397394A CN102634514B (en) | 2012-02-21 | 2012-02-21 | Bay scallop heat-resistance-related metallothionein gene marker and assisted breeding method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100397394A CN102634514B (en) | 2012-02-21 | 2012-02-21 | Bay scallop heat-resistance-related metallothionein gene marker and assisted breeding method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102634514A true CN102634514A (en) | 2012-08-15 |
| CN102634514B CN102634514B (en) | 2013-12-04 |
Family
ID=46619091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100397394A Active CN102634514B (en) | 2012-02-21 | 2012-02-21 | Bay scallop heat-resistance-related metallothionein gene marker and assisted breeding method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102634514B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021061826A (en) * | 2019-10-16 | 2021-04-22 | 中国海洋大学 | Combination of female scallop-specific molecule markers and application thereof |
| CN117327809A (en) * | 2023-12-01 | 2024-01-02 | 中国海洋大学 | Bay scallop accumulated carotenoid related molecular marker and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1793358A (en) * | 2005-09-23 | 2006-06-28 | 中国海洋大学 | Process for structure of standard microstatellite mark of bay scallop and application thereof |
| CN101255477A (en) * | 2008-04-02 | 2008-09-03 | 中国科学院海洋研究所 | Polymorphism mark screening of chlamys ferrari G-type lysozyme gene and auxiliary breeding means |
-
2012
- 2012-02-21 CN CN2012100397394A patent/CN102634514B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1793358A (en) * | 2005-09-23 | 2006-06-28 | 中国海洋大学 | Process for structure of standard microstatellite mark of bay scallop and application thereof |
| CN101255477A (en) * | 2008-04-02 | 2008-09-03 | 中国科学院海洋研究所 | Polymorphism mark screening of chlamys ferrari G-type lysozyme gene and auxiliary breeding means |
Non-Patent Citations (2)
| Title |
|---|
| WANG L等: "Alteration of metallothionein mRNA in bay scallop Argopecten irradians under cadmium exposure and bacteria challenge", 《COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY PART C:TOXICOLOGY & PHARMACOLOGY》, vol. 149, no. 1, 31 January 2009 (2009-01-31), pages 50 - 57 * |
| 刘维青等: "海湾扇贝(Argopecten irradians)金属硫蛋白基因的克隆与分析", 《海洋与湖沼》, vol. 37, no. 05, 30 September 2006 (2006-09-30), pages 444 - 449 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021061826A (en) * | 2019-10-16 | 2021-04-22 | 中国海洋大学 | Combination of female scallop-specific molecule markers and application thereof |
| JP7072277B2 (en) | 2019-10-16 | 2022-05-20 | 中国海洋大学 | Scallop sex judgment method |
| CN117327809A (en) * | 2023-12-01 | 2024-01-02 | 中国海洋大学 | Bay scallop accumulated carotenoid related molecular marker and application thereof |
| CN117327809B (en) * | 2023-12-01 | 2024-03-22 | 中国海洋大学 | Bay scallop accumulated carotenoid related molecular marker and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102634514B (en) | 2013-12-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106701926B (en) | Eggplant fruit color epistatic gene D positioning and its InDel molecular markers developments and application | |
| CN110747279B (en) | Fugu obscurus SNP molecular marker and application thereof in genetic breeding | |
| Vogel et al. | Brachypodium distachyon, a new model for the Triticeae | |
| CN109097387A (en) | A kind of methods and applications with CRISPR/Cas9 gene editing system initiative purple fruit Tomato mutants | |
| CN103993011A (en) | Molecular marker of sesame dominant genic male sterility gene and preparation method and application thereof | |
| CN107267523A (en) | A kind of bacterial leaf spot resistance albumen and encoding gene | |
| Char et al. | Use of CRISPR/Cas9 for targeted mutagenesis in sorghum | |
| CN107099588B (en) | Development and application of SSR (simple sequence repeat) marker for identifying earliness of upland cotton | |
| CN102634514B (en) | Bay scallop heat-resistance-related metallothionein gene marker and assisted breeding method thereof | |
| CN103361340B (en) | Bay scallop thermostable related heat shock protein 70 gene marker and assistant breeding method thereof | |
| CN102690812B (en) | Molecular marker SIsv0067 closely linked with millet Heading date gene | |
| CN106119360A (en) | A kind of SCAR molecular marker identifying banana blight resistance and authentication method thereof | |
| CN102492721A (en) | Sesame genetic transformation method mediated by agrobacterium | |
| Galambos et al. | Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance | |
| Carelli et al. | Reverse genetics in Medicago truncatula using a TILLING mutant collection | |
| CN104404156B (en) | Rapid identification molecular marker of self-compatible variety of loquat, marker primer and identification method | |
| CN110106275B (en) | InDel molecular marker closely linked with tea purple buds and application thereof | |
| CN102911941B (en) | Root-specific promoter and application thereof | |
| CN102690818B (en) | Molecular marker SIsv0832 closely linked with millet Heading date gene | |
| CN107287278B (en) | Recombinant nucleic acid fragment RecCR010169 and detection method thereof | |
| Falistocco et al. | Cytogenetic characterization by in situ hybridization techniques and molecular analysis of 5S rRNA genes of the European hazelnut (Corylus avellana) | |
| CN108411026B (en) | Chrysanthemum cinnamon flower type molecular marker-assisted selection method | |
| CN101633936B (en) | Fish transgenosis breeding method | |
| CN101748211A (en) | Molecular marker BoRAAG/CTC112 interlocked with head cabbage downy mildew resistance gene and acquisition method thereof | |
| CN104293918B (en) | Utilize the method that RT-PCR method carries out the most strong Fruit variety of silkworm |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |