CN102633876B - Synthetic method and application of paraquat antigen - Google Patents
Synthetic method and application of paraquat antigen Download PDFInfo
- Publication number
- CN102633876B CN102633876B CN201210081393.4A CN201210081393A CN102633876B CN 102633876 B CN102633876 B CN 102633876B CN 201210081393 A CN201210081393 A CN 201210081393A CN 102633876 B CN102633876 B CN 102633876B
- Authority
- CN
- China
- Prior art keywords
- paraquat
- add
- methyl
- bis
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention provides a synthetic method of paraquat antigen, and a direct competition ELISA (enzyme-linked immunosorbent assay) kit building method for detecting paraquat by polyclonal antibody. The kit comprises a paraquat enzyme label, paraquat standard liquid, an enzyme labeled plate coating paraquat polyclonal antibody, substrate coloration solution, cleaning solution, stop solution, enzyme label diluent, sample diluent and the like. The method for artificially synthetizing the paraquat antigen is established, and the polyclonal antibody is prepared by using BSA (bovine serum albumin) as carrier. The ELISA kit prepared using the polyclonal antibody has the advantages of simplicity, quickness, high sample processing capacity, high flexibility, high specificity, low cost and the like and suitable for factory production, and can be used for quick detection of paraquat in agricultural products such as soybean and the like.
Description
Technical field
The present invention relates to a kind of method of synthetic Paraquat antigen, the direct competitive ELISA test kit of the detection Paraquat of application polyclonal antibody art development is especially provided, also disclose its preparation method simultaneously, belong to Enzyme-multiplied immune technique field.
Background technology
Paraquat (paraquat, PQ), chemistry by name 1,1 '-dimethyl-4,4 '-bipyridine cation salt, is a kind of bipyridyliums steriland herbicide, is widely used in gardens weeding, bare place chemical weed control, can kill most of Gramineae and broadleaf weeds, greenery contact liquid just starts withered after a few hours.After liquid contact soil can by soil colloid rapidly, strong adsorption, and passivation and do not affect crop root completely.
After people's Paraquat acute poisoning, pulmonary capillary damage, alveolar edema, be finally that multiple organ failure occurs the acute explosive paraquat poisoning of pulmonary fibrosis (> 40ml) patient very soon, and mortality ratio is up to 100%; Middle severe intoxication (20～40ml) can cause acute renal failure, and severe intoxication also can Complicated with Pulmonary fibrosis and toxic hepatitis, and more than death in 2～3 weeks after poisoning; And calomel poisoning person (< 20ml) shows as oral cavity and gastrointestinal tract mucous burning more, symptom has nausea,vomiting,diarrhea, stomach, and majority can be fully recovered.
Abroad as far back as the seventies in last century, with regard to starting, the detection method of Paraquat is studied, research work in this respect has in recent years obtained progress faster.Highly sensitive instrumental method and fast and effectively immunoassay are applied to the detection of Paraquat just gradually.Domestic starting late, and be not given sufficient attention for the rapid detection of Paraquat in food.Domestic starting late, and be not given sufficient attention for the rapid detection of Paraquat.Current existing measuring method can be summarized as three classes: live organism analytical method, instrumental method, immunochemical analyses method.Organism analytical method is the species to toxic compounds sensitivity growing environment from nature screening, as the instrument of environmental monitoring.Rioboo etc. are grand by Paraquat and second third in freshwater microalgae C.Moewusii and C.Vulgaris detection water.Minimal detectable concentration is decided to be makes growth rate reduce by 50% concentration (EC50), and Paraquat is 0.28 μ mol/L to the EC50 of C.Moewusii, and second third is grand is 0.15 μ mol/L to the EC50 of C.Vulgaris.For biology general life cycle of testing short, easily operation, testing cost is low.Can be considered as a kind of big area ECOLOGICAL ENVIRONMENTAL MONITORING means in basic unit's application.Khan SU utilizes gas-chromatography (GC) to detect the Paraquat in lettuce, radish and onion, with the PQ in 5M H2SO4 extraction sample and to extract shortening, then carries out carrying out GC detection after alumina column purifying.When mark-on 0.5,0.1 and 0.05ppm, the rate of recovery is 75～86%, and absolute error is up to 4%, and minimum detectability is in 0.05ppm left and right.Liquid chromatography is applicable to detect poor heat stability, polarity is strong, molecular weight greatly, not volatile compound and ionic compound.Paraquat is the ionic compound that a kind of polarity is very strong, is relatively applicable to analyzing with HPLC.Since the eighties in 20th century, liquid phase chromatography has occupied dominant position, and and mass spectrometry, be widely used in the detection of Paraquat in the biological materials such as water body, human plasma and serum.Have scholar directly to adopt ultraviolet spectrophotometry to measure, detect and be limited to 0.01 μ g/mL, the rate of recovery is 90.0%～102.4%, easy and simple to handle fast, be suitable for the mensuration of Paraquat concentration in liver clinically.Masafumi Tomita utilizes capillary electrophoresis to detect paraquat (PQ) and the diquat (DQ) in serum simultaneously, and detection sensitivity is 0.05 μ g/mL.After this more than ten years, many foreign scholars are the detection for sample Paraquats such as serum, water by capillary electrophoresis.Because the boiling point of Paraquat is higher, polarity is strong, need to carry out after special derivative or cracking is processed just detecting to sample, operation more complicated is more difficult with gas chromatographic analysis.Therefore the report that, utilizes GC to detect is less.Thin layer chromatography can utilize special developer to observe spot colors and utilize Rf value next qualitative, and it is measured, and accuracy is poor, and detection sensitivity is not high, therefore can only be for semi-quantitative analysis.
Immunoassay is to take the specific binding of antigen and antibody to be basis, and its principle is to utilize biomolecules identification, and highly sensitive, high specificity, therefore there is extremely important status on clinical medicine.Euzymelinked immunosorbent assay (ELISA) (Enzyme Immunoassay, EIA) is a kind of immune analysis method that the efficient catalytic effect of the specific immune response of antigen, antibody and enzyme is combined.Detection principle and the radioimmunology of enzyme immunoassay are similar, and the vigor that is incorporated into the enzyme of solid phase by mensuration is measured the amount of determinand.Enzyme as marker has horseradish peroxidase (Horseradish Peroxidase, HRP) and alkaline phosphatase (Alkaline Phosphatase).Enzyme marking reagent preparation is easy, stable, inexpensive.The sensitivity of enzyme immunoassay approaches radioimmunoassay technique, and can do quantitative assay by means of simple instrument, is most widely used immuno analytical method in current agricultural chemicals monitoring.
The problem that the ELISA detection kit of Paraquat of the present invention has avoided existing detection means to exist, compare other detection means, the Paraquat ELISA kit test method of setting up has high specificity, susceptibility is high, sense cycle is short, simple to operation, the feature that can produce in a large number etc.
Summary of the invention
The present invention discloses a kind of method of synthetic Paraquat antigen, can be used for preparing polyclonal antibody.
The present invention also provides application polyclonal antibody to detect the preparation method of the direct competitive ELISA detection kit of Paraquat, is applicable to batch production and produces.
Synthetic Paraquat antigen disclosed by the invention obtains by the following method:
The preparation of immunizing antigen PQ-h-BSA: 43mgBSA is dissolved in 3ml water, with the NaOH of 0.1M, adjusts pH to 9.0.9mgN-methyl-N '-valeric acid base-bis-pyridylium compounds (PQ-h) are dissolved in 500 μ l dioxs, carry out supersound process.Add anhydrous tri-n-butylamine 15 μ l, this mixture is cooling in 12 ℃ of ice baths, and stir 15min.Add the isobutyl chlorocarbonate 8 μ l that heavily steam, 15 ℃ of following 30min that stir.PQ-h solution after stirring is dropwise joined in the albumen of dissolving, and in 4 ℃ of gentle agitation 4h, whole process maintains pH 9.0.Then, 72h that mixture is dialysed in 4 ℃ of PBS at 0.1M,
every 6hchange dialyzate once.After lyophilize in-20 ℃ of preservations.
The preparation of envelope antigen PQ-h-OVA: 28mg OVA is dissolved in 3ml water, adjusts pH to 9.0 with the NaOH of 0.1M.9mgN-methyl-N '-valeric acid base-bis-pyridylium compounds (PQ-h) are dissolved in 500 μ l dioxs, carry out supersound process.Add anhydrous tri-n-butylamine 15 μ l, this mixture is cooling in 12 ℃ of ice baths, and stir 15min.Add the isobutyl chlorocarbonate 8 μ l that heavily steam, 15 ℃ of following 30min that stir.PQ-h solution after stirring is dropwise joined in the OVA of dissolving, and in 4 ℃ of gentle agitation 4h, whole process maintains pH 9.0.Then, 72h that mixture is dialysed in 4 ℃ of PBS at 0.1M,
every 6hchange dialyzate once.After lyophilize in-20 ℃ of preservations.
The synthetic method of N-methyl-N '-valeric acid base-bis-pyridylium compounds (PQ-h):
4,4-dipyridyl and methyl iodide (CH
3i) with mol ratio, in anhydrous propanone react at 1.05: 1,4 ℃, magnetic agitation, in the dark reaction under the logical nitrogen protection of airtight three neck round-bottomed flasks.After reaction mixture adds completely, low temperature (4 ℃ of left and right) stirs and spends the night.Filter, with anhydrous propanone washing, drain, obtain product N-methyl-bis-pyridylium compounds (MQ), vacuum-drying, obtains yellow needle crystal, then deposits in vacuum drier.The MQ obtaining and 5-bromine Valeric acid ethylester are mixed with mol ratio at 1: 2,120 ℃ of oil baths, magnetic agitation, backflow 5h in the anhydrous dimethyl formamide (DMF) of fresh distillation, then room temperature is placed and is spent the night.Suction filtration, with dry DMF washing, vacuum-drying, obtains yellow plate crystal N-methyl-N '-Valeric acid ethylester base-bis-pyridylium compound, deposits in vacuum drier.N-methyl-N '-Valeric acid ethylester base-bis-pyridylium the compound obtaining is mixed to reflux 3h left and right with appropriate dense HCl.On rotatory evaporator, boil off excessive acid and water, keep bath temperature to be no more than 60 ℃, evaporate to dryness, obtains solid residue.After cooling, add a small amount of acetone crystallization (separating out white crystal), filter, drain, vacuum-drying, obtains crude product, then through 95% ethyl alcohol recrystallization, vacuum-drying, obtains high purity N-methyl-N '-valeric acid base-bis-pyridylium compound.
The present invention also provides a kind of ELISA detection kit, and particularly it is composed as follows:
Enzyme plate, substrate nitrite ion, washings, stop buffer, enzyme mark thing diluent and the sample diluting liquid etc. of Paraquat enzyme mark thing, Paraquat reference liquid, coated Paraquat polyclonal antibody.
Described polyclonal antibody is for take Paraquat as haptens, and by the synthetic holoantigen of appeal method, and by new zealand white rabbit immunity preparation, concrete grammar is as follows:
1, antiserum(antisera) preparation
The last week is made negative control from ear edge vein exploitating blood in immunity.Take 1mg PQ-h-BSA, with the PBS (through autoclaving) of 5mL 0.1mol/L, dissolve, then get the antigen that 0.5mL dissolves and fully mix with the complete freund adjuvant of 0.5mL, after emulsification for first immunisation.Initial immunity carries out multiple spot subcutaneous injection at new zealand white rabbit back, and 1mL/ only.After one month, carry out booster immunization for the first time, adopt the inner intramuscular injection of four limbs, dosage is identical with first immunisation, mixes fully emulsified with the incomplete freund adjuvant of equivalent.Booster immunization is 4 times altogether, every minor tick 2 weeks.Before last 2 booster immunizations, from rabbit ear vein blood sampling, adopt indirect elisa method to measure sero-fast tiring.Tire qualified after, the bloodletting of rabbit carotid artery, the blood gathering is standing under room temperature, after blood aggegation, put into 37 ℃ of standing 1h of insulation can, after 4 ℃ of standing over night, after 4 ℃, the centrifugal 20min of 3000rpm, obtain supernatant liquor and be antiserum(antisera).The serum amount of every rabbit is greatly about 35mL.
2, antiserum(antisera) purifying
Adopt saturated ammonium sulphate two step precipitator method antibody purifications.
1) get 10mL serum in 50mL centrifuge tube, add 10mL pH 7.0PBS, under vibrating gently, dropwise add the saturated ammonium sulphate 20mL of 4 ℃ of precoolings on ice, standing over night in 4 ℃ of refrigerators, now the final concentration of ammonium sulfate is 50%.
2) in 4 ℃ of centrifugal 25min of 4000r/min, abandon supernatant, with 6mL pH 7.0PBS dissolution precipitation, more dropwise add saturated ammonium sulphate 4mL, 4 ℃ of standing 1h, now the whole saturation ratio of ammonium sulfate is 40%.
3) repeating step 2) centrifugal, with 6.7mLpH 7.0PBS dissolution precipitation, more dropwise add saturated ammonium sulphate 3.3mL, 4 ℃ of standing 1h, now the whole saturation ratio of ammonium sulfate is 33%.
4) repeating step 2) centrifugal, precipitation is dissolved with 6mL PBS, packs in dialysis tubing.
5) in 4 ℃ of refrigerators, to 20 times of above PBS dialysis, during change liquid for several times, until with Nai Shi reagent detection dialyzate without yellow.
6) by packing after the solution lyophilize of having dialysed, in-20 ℃, save backup.
3, the making of indirect competition typical curve
1) get enzyme plate, the PQ-h-OVA diluent that is coated with concentration with the best is coated with, 100 μ L/ holes, and 4 ℃ of Refrigerator stores spend the night.
2) washing lotion washing is 1 time, and each 2min, pats dry.
3) to seal containing 3%BSA-PBS solution, 200 μ L/ holes, 37 ℃ of incubation 1.5h.
4) washing lotion washing is 3 times, and each 1min, pats dry.
5) add 0ng/mL, 0.001ng/mL, 0.01ng/mL, 0.1ng/mL, 0.5ng/mL, 1ng/mL, 2.5ng/mL, 5ng/mL, 10ng/mL, 20ng/mL, 50ng/mL, the PQ standard substance of 100ng/mL, 50 μ L/ holes; The antibody that adds again optimum dilution degree, 50 μ L/ holes, do not arrange parallel, 37 ℃ of incubation 1h.
6) washing lotion washing is 3 times, and each 3min, pats dry.
7) add the ELIAS secondary antibody (goat anti-rabbit igg-HRP) of dilution in 1: 5000,100 μ L/ holes, 37 ℃ of incubation 0.5h.
8) after washing, pat dry, add nitrite ion, 100 μ L/ holes, dark place reaction 15min.
9) add stop buffer (2molL-1 sulfuric acid), 50 μ L/ holes.
10) in microplate reader, measure 450nm light absorption value.
Take B/B0 as ordinate zou, and the logarithmic value of concentration of standard solution of 100 times of take is mapped on EXCEL as X-coordinate, obtains the competition test curve of PQ.Scope to typical curve intercepts, and matching typical curve equation.
Described Paraquat enzyme mark thing: horseradish peroxidase; Substrate nitrite ion: A liquid (0.1mol/L aqueous citric acid solution): citric acid 21.01g, distilled water is settled to 1000mL; B liquid (0.2mol/LNa2HPO4): 71.6g Na2HPO412H2O, distilled water is settled to 1000mL after dissolving, and saves backup.During use, 24.3mLA liquid mixes with 25.7mLB liquid, adds 50mL distilled water and 30%H2O218.3 μ L; Washings: containing the PBS solution of 0.05%Tween-20; The H2SO4 solution of stop buffer: 2molL-1; The Na2CO3-NaHCO3 damping fluid (CBS) of antigenic dilution: 0.05M, pH9.6: 0.315g Na2CO3,0.588g NaHCO3, adds 100ml ultrapure water and dissolves; Antibody diluent: 0.01mol/L, pH7.4 phosphate buffered saline buffer (PBS, containing 1%BSA): 3.0g Na2HPO4.12H2O, 0.25g NaH2PO42H2O, 8.7g NaCl, 10g BSA, 1L ultrapure water.
On the other hand, the present invention also provides the method that detects Paraquat content in sample, and step is as follows:
(1) sample pre-treatments
(2) by ELISA detection kit claimed in claim 2, detect, to the polyclonal enzyme plate of coated Paraquat hole, add standard substance or sample solution, add Paraquat enzyme mark thing, hatch 30 minutes, washings washing, add nitrite ion to hatch 20 minutes, stop buffer termination reaction, measures absorbance by microplate reader.
(3) analyzing and testing result
The present invention manually principle of synthetic Paraquat complete antigen is:
Take 4,4'-Bipyridine and methyl iodide as starting raw material, under logical nitrogen protection, in dark place, through three-step reaction, synthesized Paraquat haptens; Mixed anhydride method coupling high molecular weight protein carrier bovine serum albumin (BSA) and ovalbumin (OVA) are prepared immunizing antigen and envelope antigen.
The detection principle of test kit of the present invention is:
By the polyclonal antibody of anti-Paraquat coated with solid phase carrier on, add sample or Paraquat standard solution and add Paraquat enzyme mark thing, Paraquat in sample to be checked and the competition of Paraquat enzyme mark thing are coated in the polyclonal antibody on solid phase carrier, by washing, remove unconjugated Paraquat enzyme mark thing, add the rear termination reaction of nitrite ion colour developing, detect sample absorbance, in this value and sample, the amount of Paraquat is negative correlation, relatively gets final product the concentration of Paraquat in working sample with typical curve.
Positively effect of the present invention is:
The present invention discloses a kind of method of new synthetic Paraquat antigen, for preparing monoclonal antibody or polyclonal antibody, lays a good foundation; And first Application polyclonal antibody set up a kind of direct competitive ELISA test kit that detects Paraquat, can be qualitative or quantitative analysis sample in Paraquat, be applicable to batch production and produce.Test kit of the present invention has shortened detection time, and low to the pre-treatment requirement of sample, treating processes is simple, and has been equipped with relevant sample preparation reagent, can detect rapidly gross sample simultaneously.
The all reagent of test kit of the present invention is the working fluid configuring, can direct control without processing, reduced operation steps, can be quick, the Paraquat in Sensitive Detection soybean, has avoided complicated operation, low for equipment requirements, can adapt to multiple testing environment, and multiple detection needs; And test kit sample consumption of the present invention is little, and the reagent shelf time is long, and level of automation is high, to operator's nontoxicity, environmentally safe etc.; Therefore the ELISA detection kit that the present invention's application polyclonal antibody is set up has great social effect and wide market outlook.
Accompanying drawing explanation
Fig. 1 Paraquat suppresses curve
The molecular structural formula of Fig. 2 Paraquat
Embodiment
Embodiment 1 prepares Paraquat antigen
1, Paraquat is haptenic synthetic:
The molecular structural formula of Paraquat is as Fig. 2, and this experiment is with 4,4
'-dipyridyl and methyl iodide are starting raw material, have synthesized N-methyl-N '-valeric acid base-bis-pyridine dibromide (being called for short PQ-h), for the preparation of the artificial antigen of Paraquat.Its building-up reactions divides three steps to complete.
1) N-methyl-bis-pyridylium compound---Monoquat's (MQ) is synthetic
4,4-dipyridyl and methyl iodide (CH
3i) with mol ratio, in anhydrous propanone react at 1.05: 1,4 ℃, magnetic agitation, in the dark reaction under the logical nitrogen protection of airtight three neck round-bottomed flasks.After reaction mixture adds completely, low temperature (4 ℃ of left and right) stirs and spends the night.Filter, with anhydrous propanone washing, drain, obtain product MQ, vacuum-drying, obtains yellow needle crystal, then deposits in vacuum drier.Efficiency of pcr product is about 86% as calculated.
2) N-methyl-N '-Valeric acid ethylester base-bis-pyridylium compound is synthetic
The MQ that the first step reaction obtains and 5-bromine Valeric acid ethylester mix with mol ratio at 1: 2,120 ℃ of oil baths, and magnetic agitation, backflow 5h in the anhydrous dimethyl formamide (DMF) of fresh distillation, then room temperature is placed and is spent the night.Suction filtration, with dry DMF washing, vacuum-drying, obtains yellow plate crystal, deposits in vacuum drier.Yield is 57% as calculated.
3) N-methyl-N '-valeric acid base-bis-pyridylium compound (PQ-h) is synthetic
N-methyl-N '-Valeric acid ethylester base-bis-pyridylium compound that second step is obtained mixes with appropriate dense HCl, reflux 3h left and right.On rotatory evaporator, boil off excessive acid and water, keep bath temperature to be no more than 60 ℃, evaporate to dryness, obtains solid residue.After cooling, add a small amount of acetone crystallization (separating out white crystal), filter, drain, vacuum-drying, obtains crude product, then through 95% ethyl alcohol recrystallization, vacuum-drying.Yield is 69% as calculated.
2, preparation---the mixed anhydride method of haptens-protein connector
1) 9mgPQ-h is dissolved in 500 μ l dioxs, carries out supersound process.Add anhydrous tri-n-butylamine 15 μ l, this mixture is cooling in 12 ℃ of ice baths, and stir 15min.Add the isobutyl chlorocarbonate 8 μ l that heavily steam, 15 ℃ of following 30min that stir.
2) 43mg BSA is dissolved in 3ml water, with the NaOH of 0.1M, adjusts pH to 9.0.Above-mentioned PQ-h solution is dropwise joined in the albumen of dissolving, and in 4 ℃ of gentle agitation 4h, whole process maintains pH 9.0.Then, 72h that mixture is dialysed in 4 ℃ of PBS at 0.1M,
every 6hchange dialyzate once.After lyophilize in-20 ℃ of preservations.Synthetic envelope antigen PQ-h-OVA uses the same method.
The preparation of embodiment 2 Paraquat specific polyclonal antibodies
The antigen of preparing by embodiment 1 method, as immunogen, is got 2 of male new zealand white rabbits, health, body weight 1.5～2kg.The last week is made negative control from ear edge vein exploitating blood in immunity.Take 1mg PQ-h-BSA, with the PBS (through autoclaving) of 5mL 0.1mol/L, dissolve, then get the antigen that 0.5mL dissolves and fully mix with the complete freund adjuvant of 0.5mL, after emulsification for first immunisation.Initial immunity carries out multiple spot subcutaneous injection at new zealand white rabbit back, and 1mL/ only.After one month, carry out booster immunization for the first time, adopt the inner intramuscular injection of four limbs, dosage is identical with first immunisation, mixes fully emulsified with the incomplete freund adjuvant of equivalent.Booster immunization is 4 times altogether, every minor tick 2 weeks.Concrete immune programme for children is in Table 1.Before last 2 booster immunizations, from rabbit ear vein blood sampling, adopt indirect elisa method to measure sero-fast tiring.Tire qualified after, the bloodletting of rabbit carotid artery, the blood gathering is standing under room temperature, after blood aggegation, put into 37 ℃ of standing 1h of insulation can, after 4 ℃ of standing over night, after 4 ℃, the centrifugal 20min of 3000rpm, obtain supernatant liquor and be antiserum(antisera).The serum amount of every rabbit is greatly about 35mL.
Table 1 laboratory animal immune programme for children
Adopt saturated ammonium sulphate salting-out process antibody purification, concrete steps are as follows
1) get 10mL serum in 50mL centrifuge tube, add 10mL pH 7.0PBS, under vibrating gently, dropwise add the saturated ammonium sulphate 20mL of 4 ℃ of precoolings on ice, standing over night in 4 ℃ of refrigerators, now the final concentration of ammonium sulfate is 50%.
2) in 4 ℃ of centrifugal 25min of 4000r/min, abandon supernatant, with 6mL pH 7.0PBS dissolution precipitation, more dropwise add saturated ammonium sulphate 4mL, 4 ℃ of standing 1h, now the whole saturation ratio of ammonium sulfate is 40%.
3) repeating step 2) centrifugal, with 6.7mLpH 7.0PBS dissolution precipitation, more dropwise add saturated ammonium sulphate 3.3mL, 4 ℃ of standing 1h, now the whole saturation ratio of ammonium sulfate is 33%.
4) repeating step 2) centrifugal, precipitation is dissolved with 6mL PBS, packs in dialysis tubing.
5) in 4 ℃ of refrigerators, to 20 times of above PBS dialysis, during change liquid for several times, until with Nai Shi reagent detection dialyzate without yellow.
6) by packing after the solution lyophilize of having dialysed, in-20 ℃, save backup.
Embodiment 3 Paraquat enzyme mark thing preparations
Enzyme in enzyme-labelled antigen can be horseradish peroxidase (HRP) or alkaline phosphatase (AP), is preferably HRP, and HRP-Paraquat marker adopts sodium periodate legal system standby, specific as follows:
1. taking 10mgHRP is dissolved in 1mL distilled water.
2. the 0.1M NaIO that adds 0.2mL newly to join in upper liquid
4solution, under room temperature, lucifuge stirs 20 minutes.
3. above-mentioned solution is packed in dialysis tubing into 4 ℃ of dialysed overnight of sodium-acetate buffer of 1mM pH4.4.
4. add 20 μ L0.2M pH9.5 carbonate buffer solutions, make the pH of above hydroformylation HRP be elevated to 9.0-9.5.
5.0.5mg haptens is dissolved in 1mL0.01M carbonate buffer solution, is divided into two parts, joins in the HRP of hydroformylation, and under room temperature, lucifuge stirs 2 hours gently.
6. add the 4mg/mLNaBH4 liquid that 0.1mL newly joins, mix, then put 4 ℃ 2 hours.
7. above-mentioned liquid is proceeded in dialysis tubing, to 0.15MpH7.4PBS dialysis, 4 ℃ are spent the night.
8. under agitation dropwise add equal-volume saturated ammonium sulphate, put 4 ℃ 1 hour.
9.3000rpm centrifugal half an hour, abandon supernatant.Throw out washes twice with semi-saturation ammonium sulfate, and last throw out is dissolved in the PBS of a small amount of 0.15M pH7.4.
10. appeal solution is packed in dialysis tubing, the PBS damping fluid dialysis to 0.15M pH7.4, removes after ammonium ion, and 10000rpm removes precipitation in centrifugal 30 minutes, and supernatant liquor is enzyme conjugates, adds equivalent 50% glycerine, packing in a small amount, 4 ℃ or-20 ℃ of preservations.
The foundation of embodiment 4 direct competitive ELISA methods
1. the coated concentration of how anti-the best and the best weaker concn of antibody determines
1. chessboard test scalping:
PQ standard substance concentration is respectively: 0ng/mL, 50ng/mL, 500ng/mL;
Envelope antigen concentration is respectively 0.001 μ g/mL, 0.01 μ g/mL, 0.1 μ g/mL, 1 μ g/mL;
Antibody dilution is respectively 1: 2 * 103,1: 2 * 104,1: 2 * 105.
Chessboard testing data, as table 2, can calculate the corresponding inhibiting rate of different antigen/antibody concentration combination by table 2, in Table 3.
Table 2 chessboard test-results
The inhibiting rate that the various combinations of table 3 draw
As can be seen from Table 4, the antigen/antibody concentration combination that inhibiting rate is relatively high is 0.1 μ g/mL+1: 1: 2 * 103,0.1 μ g/mL+1: 2 * 105 and 1 μ g/mL+1: 2 * 104, but associative list 4-1,0.1 μ g/mL+1: although 2 * 105 these combination inhibiting rates are high, but OD numerical value is too low, confidence level is not high; So determine to choose antigen concentration between 0.1～1 μ g/mL, antibody dilution is 1: 2 * 103～1: between 2 * 104, continue to do chessboard experiment, by each concentration refinement.
2, chessboard experiment second step screening:
PQ standard substance concentration is respectively 0ng/mL, 50ng/mL, 500ng/mL;
Antigen concentration is respectively 0.1 μ g/mL, 0.2 μ g/mL, 0.5 μ g/mL, 1 μ g/mL;
Antibody dilution is respectively 1: 5000,1: 10000,1: 20000,1: 40000.
Chessboard testing data, as table 4, can calculate the corresponding inhibiting rate of different antigen/antibody concentration combination by table 4, in Table 5.
Table 4 chessboard test-results
The inhibiting rate that the various combinations of table 5 draw
By table 4 and table 5, can tentatively be judged, antigen concentration is between 0.2 μ g/mL～0.5 μ g/mL, and antibody dilution is relatively good between 1: 5000～1: 10000, because numerical value is suitable between this, inhibiting rate is in normal range, and antigen concentration is as far as possible more low better.
3, chessboard experiment the 3rd step screening:
Standard substance concentration is respectively 0ng/mL, 0.001ng/mL, 0.01ng/mL, 0.1ng/mL, 0.5ng/mL, 1ng/mL;
Antigen concentration is respectively 0.2 μ g/mL, 0.3 μ g/mL, 0.4 μ g/mL, 0.5 μ g/mL;
Antibody dilution is respectively 1: 5000,1: 10000.
Chessboard testing data, as table 6, can calculate the corresponding inhibiting rate of different antigen/antibody concentration combination by table 6, in Table 7.
Table 6 chessboard test-results
The inhibiting rate that the various combinations of table 7 draw
Through chessboard test repeatedly, the suitableeest envelope antigen concentration of final establishment is 0.3 μ g/mL, and antibody optimum dilution degree is 1: 5000.
2. half-inhibition concentration IC50 and sensitivity determines
PQ normal concentration in mixing solutions when the R value of usining approaches 50% is as IC50, and in this experiment, when PQ concentration is 5ng/mL, R value is 49.2%, other R value relatively, and 49.2% approaches 50% most.The inhibition concentration IC50 of preliminary definite this experimental technique is 5ng/mL; Through regression equation Y=-0.1853X+0.9998 (R2=0.9992), calculate, obtaining IC50 is 4.74ng/mL.
Absorbance A the moon of having measured the parallel negative hole of 10 parts of No. 2 rabbit anti-serums by icELISA method, data are as table 8.Calculate respectively A high and level tone average and relative standard deviation SD, by calculating A the moon-2SD=1.549-2 * 0.167=1.215, according to equation of linear regression: Y=-0.1853X+0.9998 (R2=0.9992), calculate, the sensitivity that can obtain No. 2 rabbit anti-serum ELISA detection methods is 0.63ng/mL.
The data of table 8 sensitivity determination
Embodiment 5 detects the establishment of Paraquat direct competitive ELISA test kit
ELISA detection kit comprises following establishment:
Paraquat enzyme mark thing: horseradish peroxidase;
Substrate nitrite ion: A liquid (0.1mol/L aqueous citric acid solution): citric acid 21.01g, distilled water is settled to 1000mL; B liquid (0.2mol/L Na
2hPO
4): 71.6g Na
2hPO
412H
2o, distilled water is settled to 1000mL after dissolving, and saves backup.During use, 24.3mLA liquid mixes with 25.7mLB liquid, adds 50mL distilled water and 30%H
2o
218.3 μ L;
Washings: containing the PBST solution of 0.05%Tween-20;
The H of stop buffer: 2molL-1
2sO
4solution;
The Na of antigenic dilution: 0.05M, pH9.6
2cO
3-NaHCO
3;
Damping fluid (CBS): 0.315gNa
2cO
3, 0.588gNaHCO
3, add 100ml ultrapure water and dissolve;
Coated damping fluid (0.05molL-1, the carbonate buffer solution of pH 9.6): 0.315gNa
2cO
3, 0.588gNaHCO
3, add ultrapure water to 100mL;
PBS (0.01molL-1, the phosphate buffered saline buffer of pH 7.4): 8.7g NaCl, 0.25g Na H
2pO
4, 3.0gNa
2hPO
412H
2o, is dissolved in 800mL ultrapure water, and heated and stirred, after all dissolving, adds ultrapure water to 1000mL.
The residue detection of Paraquat in embodiment 6 samples
1. sample pre-treatments
Soybean sample is pulverized with pulverizer, taken respectively 4.0g and pulverize sample, be placed in 15ml centrifuge tube, add respectively 50ng/mL, 500ng/mL, 2000ng/mL Paraquat standard substance and each 4.0ml of standard substance diluent, each concentration is parallel to be done 3 times.Make Paraquat concentration in sample be respectively 0.05mg/kg, 0.5mg/kg, 2mg/kg and 0mg/kg.Mix, ultrasonic extraction 20min, then, in centrifugal 15min, gets supernatant liquor, for ELISA test kit, detects.
2. test kit detects
(1) will in the enzyme plate to coated Paraquat polyclonal antibody, add 50 μ L samples or Paraquat standardized solution, and add 50 μ L Paraquat enzyme mark things;
(2) light shaking is 30 seconds, room temperature reaction 30 minutes;
(3) washing: the liquid in the hole of inclining, with washings, fill with each hole (approximately 300 μ L), then outwell washings, 3 times repeatedly, on thieving paper, pat dry for the last time.
(4) add 50 μ L Paraquat enzyme mark things, 37 ℃ are reacted 20 minutes;
(5) add 50 μ L stop buffer termination reactions;
(6) by microplate reader, read OD450nm value.
3. interpretation of result:
Drawing standard curve, in corresponding each sample, the content of Paraquat can be read from typical curve, also can go out by regression equation calculation the content of Paraquat in sample.The absorbancy recording and the Paraquat content in sample are inversely proportional to, and can calculate by typical curve the content of Paraquat.
Claims (4)
1. a method for synthetic Paraquat antigen, is characterized in that, take Paraquat as haptens, take 4,4'-Bipyridine and methyl iodide as starting raw material, and first synthetic N-methyl-N '-valeric acid base-bis-pyridine dibromide, for the preparation of the artificial antigen of Paraquat; Concrete grammar is as follows:
1) 4,4'-Bipyridine and methyl iodide react in anhydrous propanone with mol ratio 1.05:1, and 4 ℃, magnetic agitation, in the dark reaction under the logical nitrogen protection of airtight three neck round-bottomed flasks; After reaction mixture adds completely, low temperature stirs and spends the night; Filter, with anhydrous propanone washing, drain, obtain product N-methyl-bis-pyridylium compound, vacuum-drying, obtains yellow needle crystal, then deposits in vacuum drier; N-methyl-bis-pyridylium the compound obtaining and 5-bromine Valeric acid ethylester are mixed with mol ratio 1:2,120 ℃ of oil baths, magnetic agitation, the 5h that refluxes in the anhydrous dimethyl formamide of fresh distillation, then room temperature is placed and is spent the night; Suction filtration, with dry DMF washing, vacuum-drying, obtains yellow plate crystal N-methyl-N '-Valeric acid ethylester base-bis-pyridylium compound, deposits in vacuum drier; N-methyl-N '-Valeric acid ethylester base-bis-pyridylium the compound obtaining is mixed to reflux 3h with appropriate dense HCl; On rotatory evaporator, boil off excessive acid and water, keep bath temperature to be no more than 60 ℃, evaporate to dryness, obtains solid residue; After cooling, add a small amount of acetone crystallization, filter, drain, vacuum-drying, obtains crude product, then through 95% ethyl alcohol recrystallization, vacuum-drying, obtains high purity N-methyl-N '-valeric acid base-bis-pyridylium compound;
2) 43mgBSA is dissolved in 3ml water, with the NaOH of 0.1M, adjusts pH to 9.0; 9mgN-methyl-N '-valeric acid base-bis-pyridylium compound is dissolved in 500 μ l dioxs, carries out supersound process; Add anhydrous tri-n-butylamine 15 μ l, this mixture is cooling in 12 ℃ of ice baths, and stir 15min; Add the isobutyl chlorocarbonate 8 μ l that heavily steam, 15 ℃ of following 30min that stir; N-methyl-N '-valeric acid base-bis-pyridylium compound solution after stirring is dropwise joined in the BSA albumen of dissolving, and in 4 ℃ of gentle agitation 4h, whole process maintains pH 9.0; Mixture is dialysed in 4 ℃ of PBS at 0.1M 72h, changes dialyzate once every 6h, after lyophilize, in-20 ℃ of preservations, obtains PQ-h-BSA immunizing antigen;
3) 28mgOVA is dissolved in 3ml water, with the NaOH of 0.1M, adjusts pH to 9.0; 9mgN-methyl-N '-valeric acid base-bis-pyridylium compound is dissolved in 500 μ l dioxs, carries out supersound process; Add anhydrous tri-n-butylamine 15 μ l, this mixture is cooling in 12 ℃ of ice baths, and stir 15min; Add the isobutyl chlorocarbonate 8 μ l that heavily steam, 15 ℃ of following 30min that stir; N-methyl-N '-valeric acid base-bis-pyridylium compound solution after stirring is dropwise joined in the OVA of dissolving, and in 4 ℃ of gentle agitation 4h, whole process maintains pH 9.0; Mixture is dialysed in 4 ℃ of PBS at 0.1M 72h, changes dialyzate once every 6h, after lyophilize, in-20 ℃ of preservations, obtains PQ-h-OVA immunizing antigen.
2. an application rights requires the ELISA test kit that described in 1 prepared by method, it is characterized in that, composed as follows: enzyme plate, substrate nitrite ion, washings, stop buffer, enzyme mark thing diluent and the sample diluting liquid of Paraquat enzyme mark thing, Paraquat reference liquid, coated Paraquat polyclonal antibody.
3. test kit described in claim 2, is characterized in that, construction process is as follows:
One) by new zealand white rabbit passing immunization serum, take 1mgPQ-h-BSA, with the PBS of 5mL0.1mol/L, dissolve, then the antigen of getting 0.5mL dissolving fully mixes with the complete freund adjuvant of 0.5mL, new zealand white rabbit is injected, adopt indirect elisa method to measure sero-fast tiring, tire qualified after, the bloodletting of rabbit carotid artery, the blood gathering is standing under room temperature, after blood aggegation, put into 37 ℃ of standing 1h of insulation can, after 4 ℃ of standing over night, after 4 ℃, the centrifugal 20min of 3000rpm, obtain supernatant liquor and be antibody serum;
Two) adopt saturated ammonium sulphate two step precipitator method antibody purifications
1) get 10mL serum in 50mL centrifuge tube, add 10mLpH7.0PBS, under vibrating gently, dropwise add the saturated ammonium sulphate 20mL of 4 ℃ of precoolings on ice, standing over night in 4 ℃ of refrigerators, now the final concentration of ammonium sulfate is 50%;
2) in 4 ℃ of centrifugal 25min of 4000r/min, abandon supernatant, with 6mLpH7.0PBS dissolution precipitation, more dropwise add saturated ammonium sulphate 4mL, 4 ℃ of standing 1h, now the whole saturation ratio of ammonium sulfate is 40%;
3) repeating step 2) centrifugal, use 6.7mLpH7.0PBS dissolution precipitation, more dropwise add saturated ammonium sulphate 3.3mL, 4 ℃ of standing 1h, now the whole saturation ratio of ammonium sulfate is 33%;
4) repeating step 2) centrifugal, precipitation is dissolved with 6mLPBS, packs in dialysis tubing;
5) in 4 ℃ of refrigerators, to 20 times of above PBS dialysis, during change liquid for several times, until with Nai Shi reagent detection dialyzate without yellow;
6) by packing after the solution lyophilize of having dialysed, in-20 ℃, save backup;
Three) making of indirect competition typical curve
1) get enzyme plate, the PQ-h-OVA diluent that is coated with concentration with the best is coated with, 100 μ L/ holes, and 4 ℃ of Refrigerator stores spend the night;
2) washing lotion washing is 1 time, and each 2min, pats dry;
3) to seal containing 3%BSA-PBS solution, 200 μ L/ holes, 37 ℃ of incubation 1.5h;
4) washing lotion washing is 3 times, and each 1min, pats dry;
5) add 0ng/mL, 0.001ng/mL, 0.01ng/mL, 0.1ng/mL, 0.5ng/mL, 1ng/mL, 2.5ng/mL, 5ng/mL, 10ng/mL, 20ng/mL, 50ng/mL, the PQ standard substance of 100ng/mL, 50 μ L/ holes; The antibody that adds again optimum dilution degree, 50 μ L/ holes, do not arrange parallel, 37 ℃ of incubation 1h;
6) washing lotion washing is 3 times, and each 3min, pats dry;
7) add the ELIAS secondary antibody of 1:5000 dilution, 100 μ L/ holes, 37 ℃ of incubation 0.5h; Described ELIAS secondary antibody is goat anti-rabbit igg-HRP;
8) after washing, pat dry, add nitrite ion, 100 μ L/ holes, dark place reaction 15min;
9) add stop buffer, 50 μ L/ holes; Described stop buffer is 2molL
-1sulfuric acid;
10) in microplate reader, measure 450nm light absorption value, take B/B0 as ordinate zou, and the logarithmic value of concentration of standard solution of 100 times of take is mapped on EXCEL as X-coordinate, obtains the competition test curve of PQ, scope to typical curve intercepts, and matching typical curve equation.
4. the detection method of ELISA test kit described in claim 2, comprises the following steps:
1) soybean sample pre-treatment: soybean sample is pulverized with pulverizer, take respectively 4.0g and pulverize sample, be placed in 15ml centrifuge tube, add respectively 50ng/mL, 500ng/mL, 2000ng/mL Paraquat standard substance and each 4.0ml of standard substance diluent, each concentration is parallel to be done 3 times, make Paraquat concentration in sample be respectively 0.05mg/kg, 0.5mg/kg, 2mg/kg and 0mg/kg, mix, ultrasonic extraction 20min, then in centrifugal 15min, get supernatant liquor, for ELISA test kit, detect;
2) with ELISA test kit claimed in claim 2, detect, to being coated with in the how anti-enzyme plate hole of Paraquat, add standard substance or sample solution, add Paraquat enzyme mark thing, hatch 30 minutes, washings washing, add nitrite ion to hatch 20 minutes, stop buffer termination reaction, measures absorbance by microplate reader;
3) analyzing and testing result: the absorbancy recording and the Paraquat content in sample are inversely proportional to, can calculate by typical curve the content of Paraquat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210081393.4A CN102633876B (en) | 2012-03-26 | 2012-03-26 | Synthetic method and application of paraquat antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210081393.4A CN102633876B (en) | 2012-03-26 | 2012-03-26 | Synthetic method and application of paraquat antigen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102633876A CN102633876A (en) | 2012-08-15 |
| CN102633876B true CN102633876B (en) | 2014-10-22 |
Family
ID=46618466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210081393.4A Expired - Fee Related CN102633876B (en) | 2012-03-26 | 2012-03-26 | Synthetic method and application of paraquat antigen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102633876B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103454411B (en) * | 2013-05-27 | 2015-08-12 | 广东省农业科学院动物卫生研究所 | The preparation method of a kind of biotin labeling rabbit anti-tilapia IgM polyclonal antibody and application |
| CN106568961A (en) * | 2015-10-13 | 2017-04-19 | 镇江先创生物科技有限公司 | Enzyme linked immunosorbent assay kit for detection of paraquat and detection method thereof |
| CN110981791A (en) * | 2019-11-06 | 2020-04-10 | 苏州博源医疗科技有限公司 | Paraquat derivative, preparation method thereof and paraquat detection reagent |
-
2012
- 2012-03-26 CN CN201210081393.4A patent/CN102633876B/en not_active Expired - Fee Related
Non-Patent Citations (7)
| Title |
|---|
| 半抗原:N-甲基-N′-戊酸基-二吡啶-二溴化物的合成;马兆扬等;《卫生研究》;20041231;第33卷(第2期);第221页2.2实验过程 * |
| 孙秀兰等.食品中百草枯3种检测方法的比较.《食品与生物技术学报》.2010,第29卷(第6期),第884页1.2.1免疫学检测方法的应用. |
| 杨婷婷等.百草枯人工抗原的合成及抗体的制备.《细胞与分子免疫学杂志》.2008,第24卷(第9期),摘要，第924页1.2.1，第924页1.2.4至第925页1.2.5，第925页2.1百草枯半抗原的合成结果. |
| 王廷华等.抗体的分离纯化 盐析法.《抗体理论与技术》.2005,第203-204页. * |
| 百草枯人工抗原的合成及抗体的制备;杨婷婷等;《细胞与分子免疫学杂志》;20081231;第24卷(第9期);摘要，第924页1.2.1，第924页1.2.4至第925页1.2.5，第925页2.1百草枯半抗原的合成结果 * |
| 食品中百草枯3种检测方法的比较;孙秀兰等;《食品与生物技术学报》;20101130;第29卷(第6期);第884页1.2.1免疫学检测方法的应用 * |
| 马兆扬等.半抗原:N-甲基-N′-戊酸基-二吡啶-二溴化物的合成.《卫生研究》.2004,第33卷(第2期),第221页2.2实验过程. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102633876A (en) | 2012-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101571539B (en) | Elisa kit for detecting cephalo-type medicine and application thereof | |
| CN101776685B (en) | Enzyme linked immunosorbent assay kit for detecting trimethoprim medicament and application thereof | |
| CN101463086B (en) | Diethoxy thiophosphate organophosphorus pesticide antibody, and preparation and use thereof | |
| CN102719405B (en) | Hybridoma cell strain 1C8 and anti-aflatoxin G1 monoclonal antibody produced by same | |
| CN103951577A (en) | Artificial hapten and artificial antigen of capsaicine, as well as preparation methods thereof | |
| CN104655847A (en) | Enzyme linked immunosorbent assay kit (ELISA kit) for detecting adprin and detection method thereof | |
| CN101799469A (en) | Enzyme linked immunosorbent assay kit for detecting residual clenbuterol hydrochloride and a detection method thereof | |
| CN104327183A (en) | Preparation method and application of paclobutrazol artificial antigen and polyclonal antibody | |
| CN101477123B (en) | ELISA reagent kit for detecting two oxyethyl group thiophosphate organophosphorus pesticide | |
| CN105294862A (en) | Preparation method for high-specificity difenoconazole polyclonal antibody | |
| CN102633876B (en) | Synthetic method and application of paraquat antigen | |
| CN103630689A (en) | ELISA (Enzyme-linked Immunosorbent Assay) kit used for detecting Cimaterol medicine residue, as well as preparation method and application of ELISA kit | |
| CN103613563A (en) | Preparation method of benzothiostrobin hapten, artificial antigen and specific antibody and application thereof | |
| CN101538307A (en) | Dehydroepiandrosterone hapten, artificial antigen and antibody as well as preparation method and application thereof | |
| CN105399639A (en) | Tyramine artificial antigen and antibody, and preparation methods and application thereof | |
| CN101526525A (en) | Enzyme-linked immunosorbent assay kit suitable for pentachlorophenol residual analysis | |
| CN101135683B (en) | Bifenthrin antigen, antibody and uses thereof | |
| CN103267842B (en) | Detect the immune colloidal gold method of the illegal interpolation Diclofenac of Chinese patent drug | |
| CN106771150A (en) | A kind of ELISA detection kit and detection method for detecting dimethomorph residual | |
| CN102372776B (en) | Synthesis method of II pyrethroid artificial antigen | |
| CN105273060A (en) | Antibody with lysine single methylated derivatization modification and preparation method thereof | |
| Tang et al. | Synthesis of an artificial antigen and preparation of a polyclonal antibody for the sensitive determination of phthalate esters by enzyme-linked immunoassay | |
| CN109824599A (en) | A kind of albendazole haptens and its preparation method and application | |
| CN101412673A (en) | Deltamethrin antigen and antibody, as well as use thereof | |
| CN101526527A (en) | ELISA kit applicable to residue analysis of terrachlor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Jinneng Inventor after: Zhang Jing Inventor after: Zhu Wenqi Inventor after: Sun Xiulan Inventor after: Zhang Yinzhi Inventor before: Sun Xiulan Inventor before: You Jiming Inventor before: Li Hongmei Inventor before: Zheng Jiayu Inventor before: Zhang Gong Inventor before: Ji Jian Inventor before: Tian Xiumei Inventor before: Zhang Yinzhi |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170717 Address after: 201620 Shanghai city Songjiang District Guangfulin road 697 Lane 21, room 1324-1 Patentee after: Shanghai xiongtu Biological Technology Co. Ltd. Address before: 1800 No. 214122 Jiangsu city of Wuxi Province Li Lake Avenue Patentee before: Jiangnan University |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141022 Termination date: 20180326 |