CN106568961A - Enzyme linked immunosorbent assay kit for detection of paraquat and detection method thereof - Google Patents
Enzyme linked immunosorbent assay kit for detection of paraquat and detection method thereof Download PDFInfo
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- CN106568961A CN106568961A CN201510656615.4A CN201510656615A CN106568961A CN 106568961 A CN106568961 A CN 106568961A CN 201510656615 A CN201510656615 A CN 201510656615A CN 106568961 A CN106568961 A CN 106568961A
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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Abstract
The invention relates to an enzyme linked immunosorbent assay kit for detection of paraquat and a detection method thereof. The kit and the detection method are sensitive, accurate and fast in detection, are simple to operate, have strong specificity, and are suitable for detection of large quantities of samples. The kit comprises a paraquat-antigen-coated enzyme label plate, a paraquat standard substance, a paraquat antibody working solution, a paraquat enzyme-labelled secondary antibody working solution, a substrate solution A, a substrate solution B, a stopping solution, a concentrated diluent and a concentrated washing liquid. The principle of the enzyme linked immunosorbent assay kit for detection of paraquat is a solid-phase indirect competitive-ELISA reaction. By adding an extracted sample, the enzyme-labelled secondary antibody working solution and the antibody working solution into corresponding micropores of the enzyme label plate, incubating for a period of time, adding the substrate solution A and the substrate solution B into a washing board, a developer turns blue under the action of enzyme; and then by adding the stopping solution, the color turns yellow from blue. Chromogenic depth is inversely proportional to the content of paraquat in the standard substance or the sample. The method can be directly used for detecting residual quantity of paraquat in crops.
Description
Technical field
The present invention relates to detection of veterinary drugs in food technical field, particularly detects the enzyme linked immunological kit of paraquat in rice.
Background technology
Paraquat, chemical name is 1-1- dimethyl -4-4- bipyridine cation salt, is a kind of quick steriland herbicide, with action of contace poison and certain systemic action.Can be absorbed by plant green tissues rapidly so as to withered.To non-green tissue without effect.Combined with soil rapidly in soil and be passivated, it is invalid to plant root and perennial subterranean stem and perennial root.But paraquat to people's toxicity greatly, and without special efficacy antidote, by more than 20 countries forbidden or strictly limited to use up to more than 90%, at present by the oral ingestion death rate.From 1 day July in 2014, the registration of revocation paraquat aqua and production permit, stopping were produced for China；But retain the outlet of Mu Yao manufacturing enterprises aquas and overseas specialize in outlet production using registering, allow, on July 1st, 2016 stops aqua and sells at home and use.Therefore periodically paraquat is remained carries out detecting the necessary means for becoming many national monitoring.
Enzyme linked immunosorbent assay is a kind of accurate, reliable, quick, special detection method, is suitable for the quick screening of gross sample, and food safety detection industry is widely used in recent years.It is contemplated that setting up a kind of enzyme linked immunological kit and its detection method of detection paraquat.
The content of the invention
In order to overcome chromatographic deficiency, the present invention to provide a kind of enzyme linked immunological kit and its detection method of detection paraquat.The method is sensitive, accurate, quick, easy to operate, high specificity, it is adaptable to the quick detection of gross sample.
The enzyme linked immunological kit of present invention detection paraquat, including ELISA Plate, paraquat standard items, paraquat antibody working solution, paraquat ELIAS secondary antibody working solution, substrate solution A, substrate solution B, terminate liquid, concentration and dilution liquid and concentrated cleaning solution.
The preparation of the enzyme linked immunological kit of present invention detection paraquat, comprises the following steps：Preparation, the preparation of paraquat standard items, the preparation of paraquat antibody working solution, the preparation of paraquat ELIAS secondary antibody working solution, the preparation of substrate solution A, the preparation of substrate solution B, the preparation of terminate liquid, the preparation of concentration and dilution liquid and the preparation of concentrated cleaning solution of ELISA Plate.
It is further characterized by：Described ELISA Plate is prepared via paraquat antigen coat, comprise the concrete steps that to be coupled paraquat haptens and the pure albumen of carrier proteins Bovine (BSA) and obtain envelope antigen, with carbonate (CBS) buffer solution of 0.05 mol/L pH 9.6 as coating buffer, paraquat envelope antigen is diluted to into 1：40000 ratios, 100 μ L/ holes, 37 DEG C of lucifuges are incubated 2 h, take out ELISA Plate and get rid of liquid in plate, add the μ L/ holes of diluted concentrated cleaning solution 300, board-washing 2 times, 30 s/ time, it is subsequently adding 0.5% bovine serum albumin(BSA) (BSA) confining liquid, 150 μ L/ holes, 37 DEG C of 1.5 h of placement, get rid of confining liquid and directly pat dry, ELISA Plate after patting dry is placed (25 DEG C) between constant temperature and is dried, and preserves under the conditions of ELISA Plate is vacuum-sealed in into 4 DEG C after sampling observation is qualified.
Paraquat standard concentration is respectively 0 mg/kg, 0.1mg/kg, 0.3 mg/kg, 0.9 mg/kg, 2.7 mg/kg, 8.1 mg/kg.
The paraquat antibody working solution is to obtain monoclonal antibody using paraquat artificial antigen immune mouse, and with antibody diluent 1 is diluted to：It is prepared by 60000 ratios.
The paraquat ELIAS secondary antibody working solution adds diluted into 1 by ELIAS secondary antibody：2000 ratios, the substrate solution A is the citrate-phosphate disodium hydrogen cushioning liquid of the carbamide peroxide containing 0.5 mmol/L, and the substrate solution B is the ethanol solution of tetramethyl biphenyl diamines, and the terminate liquid is 2
The sulfuric acid of mol/L, the concentration and dilution liquid is 10 times of concentration and dilution liquid, is the PBS of 0.1 mol/L, between pH value range 7.0-7.5, the concentrated cleaning solution is 10 times of concentrated cleaning solutions, is containing 0.5% Tween-20, the PBST of 0.1 mol/L, between pH value range 7.0-7.5.
The enzyme linked immunological kit and its detection method of detection paraquat, based on the indirect competitive enzyme-linked immunosorbent reaction principle of antigen-antibody, the method is comprised the following steps：
(1) testing sample is pre-processed, sample treatment that will be to be tested is fluid sample, or uses Solvent Extract methods testing sample, and is redissolved in sample diluting liquid；
(2) required reagent is taken out from cold storage environment, is placed in room temperature（20～25 DEG C）30 more than min are balanced, notices that every kind of liquid reagent must shake up using before；
(3) ELISA Plate for being coated with paraquat antigen is taken, plus the μ L/ holes of standard items/sample 50 are in corresponding micropore, add paraquat ELIAS secondary antibody working solution, 50 μ L/ holes, it is subsequently adding paraquat antibody working solution, 50 μ L/ holes, gently vibration is mixed, and with cover plate membrane cover plate 25 DEG C of light protected environments of rearmounted room temperature 30 min are reacted；
(4) cover plate film is carefully opened, liquid in hole is dried, with the μ L/ holes of wash operating solution 260, fully washed 4 ~ 5 times, per the s of minor tick 10, patted dry (bubble not being eliminated after patting dry can be poked with original pipette tips) with blotting paper；
(5) the μ L/ holes of substrate solution A 50, the μ L/ holes of substrate solution B 50, gently vibration is added to mix, with 15～20 min of reaction in the rearmounted 25 DEG C of light protected environments of cover plate membrane cover plate；
(6) the μ L/ holes of terminate liquid 50 are added, gently vibration is mixed, setting ELIASA is at 450 nm or the nm of dual wavelength 450/630 is detected, determines per hole absorbance（Please run through data in 5 min）；
(7) with standard concentration (ppb) logarithm as abscissa, standard items percentage absorbance be ordinate, draw calibration curve, reference standard curve calculate sample in paraquat content.
The enzyme linked immunological kit of present invention detection paraquat and its measuring principle of detection method：Paraquat in sample and antigen-specific sexual competition antibody fixed on ELISA Plate, add ELIAS secondary antibody, and antibody response, by enzymatic chromogenic reagent, according to the depth of colour developing come the content of paraquat in judgement sample.If the paraquat content in sample is few, colour developing is deep；Conversely, it is shallow then to develop the color.The kit test method of the present invention is easy to operate, detects sensitive, accurate, quick, it is adaptable to the quick detection of batch samples.
Specific embodiment
The preparation of paraquat protein conjugate：
Paraquat hapten derivant with carboxyl is obtained using succinyl oxide method, 0.05 mmol and carrier protein BSA is taken afterwards by 10：1 combination ratio is blended in 0.05
In the carbonate buffer solution (CBS) of mol/L pH 9.6, it is subsequently adding 0.15 mmol carbodiimides, the h of room temperature reaction 24 is put in stirring, dialyse two days most in the PBS of 0.2 mol/L pH 7.6, unreacted haptens is removed, the protein conjugate solution for obtaining is saved backup in -20 DEG C.
The preparation of paraquat antibody：
From the purebred BALA/C mouse of healthy adult, take to mix with equivalent complete Freund's adjuvant with the μ g of immunizing antigen 50 of protein molecule preparation and initial immunity is carried out using lumbar injection, equivalent incomplete Freund's adjuvant was added to carry out using lumbar injection with same dose immunizing antigen every 3 weeks afterwards secondary, three immunity, every time tail vein blood determines antiserum titre to certain titre after immunity 6 days, adjuvant is not added with same dose carries out final immunization, spleen is taken after 3 days preparing Spleen cell suspensions and myeloma cell carries out cell fusion, hybridoma cell line required for filtering out carries out cloning, the hybridoma in exponential phase is selected to carry out frozen, for ascites preparation, first lumbar injection 0.5
Ml atoleines are in BALB/C mouse sensitization, pneumoretroperitoneum injection 1 × 10 in 2 weeks6Individual hybridoma, inoculating cell can produce ascites after 7-10 days, ascites is extracted with syringe when ascites is as more as possible, collect repeatedly for several times, 4000
Rpm is centrifuged 15 min, collects supernatant, and monoclonal antibody is purified using caprylic acid-ammonium purifying ascites, and freeze-drying obtains freeze-dried powder and saves backup after -20 DEG C.
Preparation is coated with the ELISA Plate of paraquat envelope antigen：
Paraquat haptens and carrier proteins Bovine pure albumen (BSA) are coupled and are obtained by envelope antigen, with carbonate (CBS) buffer solution of 0.05 mol/L pH 9.6 as coating buffer, by paraquat antigen diluent into 1:40000 ratios, 100 L/ holes, 37 DEG C of 2 h of placement take out ELISA Plate and get rid of liquid in plate, and with the L/ holes of concentrated cleaning solution 300 after dilution, board-washing 2 times 30 s/ time, is subsequently adding 0.5% bovine serum albumin(BSA) (BSA) and closes, and 150
L/ holes, 37 DEG C of 1.5 h of placement, discard confining liquid, and ELISA Plate after patting dry is placed (25 DEG C) between constant temperature and dried, inspect by random samples it is qualified after by ELISA Plate vacuum sealing it is rearmounted 4 DEG C at preserve.
Paraquat standard items compound concentration is respectively 0 mg/kg, 0.1mg/kg, 0.3 mg/kg, 0.9 mg/kg, 2.7 mg/kg, 8.1 mg/kg.
The preparation of paraquat antibody working solution：Monoclonal antibody is obtained using paraquat artificial antigen immune mouse, with antibody diluent 1 is diluted to：It is prepared by 60000 ratios.
Paraquat ELIAS secondary antibody working solution adds diluted into 1 by ELIAS secondary antibody：2000 ratios, substrate solution A is the citrate-phosphate disodium hydrogen cushioning liquid of the carbamide peroxide containing 0.5 mmol/L, and substrate solution B is the ethanol solution of tetramethyl biphenyl diamines, and terminate liquid is 2
The sulfuric acid of mol/L, concentration and dilution liquid is 10 times of concentration and dilution liquid, is the PBS of 0.1 mol/L, and between pH value range 7.0-7.5, concentrated cleaning solution is 10 times of concentrated cleaning solutions, is containing 0.5% Tween-20, the PBST of 0.01mol/L, between pH value range 7.0-7.5.
Based on the reagent of above-mentioned preparation, the present invention includes following material for the enzyme linked immunological kit for detecting paraquat：
(1) 96 hole elisa Plates × 1 piece；
(2) titer × 6 bottle：(1mL/ bottles) 0 mg/kg, 0.1mg/kg, 0.3 mg/kg, 0.9 mg/kg, 2.7 mg/kg, 8.1 mg/kg；
(3) mL of antibody working solution 7；
(4) mL of ELIAS secondary antibody working solution 7；
(5) mL of substrate solution A 7；
(6) mL of substrate solution B 7；
(7) mL of terminate liquid 7；
The mL of (8) 10 × concentration and dilution liquid 40；
The mL of (9) 10 × concentrated cleaning solution 40；
When the kit of the present invention is used to detect paraquat residual quantity in crop sample, implemented by following steps：Sample pretreatment, detected with kit of the present invention, analysis result.
(1) with paraquat residual quantity in kit of the present invention detection testing sample
The ELISA Plate for being coated with paraquat antigen is taken, plus the L/ holes of standard items/sample 50 are in corresponding micropore；ELIAS secondary antibody working solution, 50 L/ holes is added to be subsequently adding paraquat antibody working solution, 50 L/ holes, gently vibration is mixed, and with cover plate membrane cover plate 25 DEG C of light protected environments of rearmounted room temperature 30 min are reacted；Cover plate film is carefully opened, liquid in hole is dried, with wash operating solution 300
L/ holes, fully washing 4 times, soak 15-30 s, are patted dry with blotting paper；Add the L/ holes of substrate solution A 50, the L/ holes of substrate solution B 50, gently vibration to mix, with the rearmounted 25 DEG C of light protected environments of cover plate membrane cover plate 15 min are reacted；The L/ holes of terminate liquid 50 are added, gently vibration is mixed, setting ELIASA is in 450
At nm or the nm of dual wavelength 450/630 detections, determine per hole absorbance（Please run through data in 5 min）；The absorbance size of contrast testing sample and standard items, paraquat residual quantity in quantitative analysis testing sample.
(2) analysis result
The percentage absorbance of the calculating of percentage absorbance, standard items or sample is equal to the absorbance of the mean value (diplopore) divided by first standard (0 standard) of the absorbance of standard items or sample, then is multiplied by 100%, i.e.,
Percentage absorbance (%)=B/B0×100%
Wherein mean absorbance values of B-standard liquid or sample solution, B0—0
The mean absorbance values of ppb standard liquids.
As abscissa, standard items percentage absorbance is ordinate to logarithm with the standard concentration (ppb) of paraquat, draws calibration curve, obtains linear equation.The percentage absorbance of sample is substituted in calibration curve, the concentration corresponding to sample is read from calibration curve, be multiplied by the actual concentrations that its corresponding extension rate is paraquat in sample.
Claims (7)
1. the enzyme linked immunological kit and its detection method of paraquat, including ELISA Plate, paraquat standard items, paraquat antibody working solution, paraquat ELIAS secondary antibody working solution, substrate solution A, substrate solution B, terminate liquid, concentration and dilution liquid and concentrated cleaning solution are detected.
2. the enzyme linked immunological kit and its detection method of paraquat are detected, is comprised the following steps：Preparation, the preparation of paraquat standard items, the preparation of paraquat antibody working solution, the preparation of paraquat ELIAS secondary antibody working solution, the preparation of substrate solution A, the preparation of substrate solution B, the preparation of terminate liquid, the preparation of concentration and dilution liquid and the preparation of concentrated cleaning solution of ELISA Plate.
3. it is according to claim 2 detection paraquat enzyme linked immunological kit and its detection method, it is characterised in that：Described ELISA Plate preparation method is to be coupled paraquat haptens and the pure albumen of carrier proteins Bovine (BSA) to obtain paraquat envelope antigen, with carbonate (CBS) buffer solution of 0.05 mol/L pH 9.6 as coating buffer, envelope antigen is diluted to into 1：40000 ratios, 100 μ L/ holes, 37 DEG C of 2 h of incubation, take out ELISA Plate and get rid of liquid in plate, add the μ L/ holes of concentrated cleaning solution 300 after dilution, board-washing 2 times, 30 s/ time, it is subsequently adding 0.5% bovine serum albumin(BSA) (BSA) closing, 150 μ L/ holes, 37 DEG C of 1.5 h of placement, discard confining liquid and directly pat dry, ELISA Plate after patting dry is placed (25 DEG C) between constant temperature and is dried, and preserves under the conditions of ELISA Plate is vacuum-sealed in into 4 DEG C after sampling observation is qualified.
4. it is according to claim 2 detection paraquat enzyme linked immunological kit and its detection method, it is characterised in that：The concentration of paraquat standard items be respectively 0 mg/kg, 0.1mg/kg, 0.3
mg/kg、0.9 mg/kg、2.7 mg/kg、8.1 mg/kg。
5. it is according to claim 2 detection paraquat enzyme linked immunological kit and its detection method, it is characterised in that：Described paraquat antibody working solution is to obtain monoclonal antibody using paraquat artificial antigen immune mouse, and with antibody diluent 1 is diluted to：It is prepared by 60000 ratios.
6. it is according to claim 2 it is a kind of detection paraquat enzyme linked immunological kit and its detection method, it is characterised in that：Described paraquat ELIAS secondary antibody working solution adds diluted into 1 by ELIAS secondary antibody：2000 ratios, the substrate solution A is the citrate-phosphate disodium hydrogen cushioning liquid of the carbamide peroxide containing 0.5 mmol/L, the substrate solution B is the ethanol solution of tetramethyl biphenyl diamines, the terminate liquid is the sulfuric acid of 2 mol/L, and the concentration and dilution liquid is 10 times of concentration and dilution liquid, and it is the PBS of 0.1 mol/L, between pH value range 7.0-7.5, the concentrated cleaning solution is 10 times of concentrated cleaning solutions, and it is containing 0.5% Tween-20 0.01
The PBST of mol/L, between pH value range 7.0-7.5.
7. the enzyme linked immunological kit and its detection method of the detection paraquat described in claim 2, based on the indirect competitive enzyme-linked immunosorbent reaction principle of antigen-antibody, the method is characterized in that：Pretreatment testing sample，Take the ELISA Plate for being coated with paraquat antigen，Sequentially it is separately added into standard items/sample、Paraquat ELIAS secondary antibody working solution、The each 50 μ L/ holes of paraquat antibody working solution are in corresponding micropore，Gently vibration is mixed，30 min are reacted with cover plate membrane cover plate 25 DEG C of light protected environments of rearmounted room temperature，Liquid in hole is dried，4 ~ 5 times are fully washed with wash operating solution，Per the s of minor tick 10，The μ L/ holes of substrate solution A 50 are added after patting dry，The μ L/ holes of substrate solution B 50，Gently vibration is mixed，With 15～20 min of reaction in the rearmounted 25 DEG C of light protected environments of cover plate membrane cover plate，Add the μ L/ holes of terminate liquid 50，Gently vibration is mixed，Setting ELIASA is at 450 nm or the nm of dual wavelength 450/630 is detected，Determine per hole absorbance（Please run through data in 5 min）, with standard concentration (ppb) logarithm as abscissa, standard items percentage absorbance be ordinate, draw calibration curve, reference standard curve calculate sample in paraquat content.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111303292A (en) * | 2020-03-06 | 2020-06-19 | 华南农业大学 | Nano antibody Nb2-37 for specifically recognizing paraquat and application thereof |
| CN113203732A (en) * | 2021-05-12 | 2021-08-03 | 淮北师范大学 | Colorimetric detection method for aryloxy phenoxy propionate herbicide based on magnetic nanocrystal enzyme preparation |
| CN117209610A (en) * | 2023-10-23 | 2023-12-12 | 华南农业大学 | Anti-paraquat nano antibody and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633876A (en) * | 2012-03-26 | 2012-08-15 | 江南大学 | Synthetic method and application of paraquat antigen |
| CN104655846A (en) * | 2013-11-20 | 2015-05-27 | 镇江先创生物科技有限公司 | Enzyme linked immunosorbent assay kit for detecting progesterone and detection method thereof |
-
2015
- 2015-10-13 CN CN201510656615.4A patent/CN106568961A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633876A (en) * | 2012-03-26 | 2012-08-15 | 江南大学 | Synthetic method and application of paraquat antigen |
| CN104655846A (en) * | 2013-11-20 | 2015-05-27 | 镇江先创生物科技有限公司 | Enzyme linked immunosorbent assay kit for detecting progesterone and detection method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111303292A (en) * | 2020-03-06 | 2020-06-19 | 华南农业大学 | Nano antibody Nb2-37 for specifically recognizing paraquat and application thereof |
| CN111303292B (en) * | 2020-03-06 | 2021-07-20 | 华南农业大学 | Nano antibody Nb2-37 for specifically recognizing paraquat and application thereof |
| CN113203732A (en) * | 2021-05-12 | 2021-08-03 | 淮北师范大学 | Colorimetric detection method for aryloxy phenoxy propionate herbicide based on magnetic nanocrystal enzyme preparation |
| CN113203732B (en) * | 2021-05-12 | 2023-02-03 | 淮北师范大学 | Colorimetric detection method for aryloxy phenoxy propionate herbicide based on magnetic nanocrystal enzyme preparation |
| CN117209610A (en) * | 2023-10-23 | 2023-12-12 | 华南农业大学 | Anti-paraquat nano antibody and application thereof |
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