CN102626138A - Gamma-reanal containing brown fermented milk base material, milk beverage and preparation methods thereof - Google Patents

Gamma-reanal containing brown fermented milk base material, milk beverage and preparation methods thereof Download PDF

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CN102626138A
CN102626138A CN2012100997463A CN201210099746A CN102626138A CN 102626138 A CN102626138 A CN 102626138A CN 2012100997463 A CN2012100997463 A CN 2012100997463A CN 201210099746 A CN201210099746 A CN 201210099746A CN 102626138 A CN102626138 A CN 102626138A
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brown
preparation
temperature
gaba
raw material
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CN102626138B (en
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韩梅
徐致远
刘振民
于鹏
沈玲
周杰
王豪
廖文艳
应杰
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Shanghai Bright Dairy and Food Co Ltd
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Shanghai Bright Dairy and Food Co Ltd
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Abstract

The invention discloses a gamma-reanal containing brown fermented milk base material and a preparation method thereof. The preparation method comprises the steps of: (1) mixing a raw material A uniformly and homogenizing to obtain a material liquid B, wherein the raw material A comprises monosaccharide, sodium glutamate and raw milk, and the material liquid B contains the sodium glutamate accounting for 7-10 g/L and the monosaccharide accounting for 60-150 g/L; (2) sterilizing the material liquid B at 90-95 DEG C for 60-120 min or sterilizing the material liquid B at 115-120 DEG C for 10-20 min; meanwhile, performing Maillard reaction and cooling; (3) grafting a leavening agent for fermentation so as to obtain the gamma-reanal containing brown fermented milk base material, wherein the fermentation time lasts for 72 h to 96 h; the leavening agent is satisfied with the condition that the raw milk is added with 10 g/L sodium glutamate and is grafted with the leavening agent according to a grafting amount of 1*106 cfu/mL, and the yield of the gamma-reanal after 72 hours fermentation is not less than 4 g/L. The preparation method greatly shortens the sterilization time. The brown fermented milk beverage prepared by using the gamma-reanal containing brown fermented milk base material has the advantages of being good in mouth feeling and flavor, containing a plenty of gamma-reanal, adding functionality and health protection property of milk products and making up the situation that the natural gamma-reanal is relatively high in price and is limited to application.

Description

Contain brown acidified milk base-material, milk beverage of GABA and preparation method thereof
Technical field
The present invention relates to a kind of brown acidified milk base-material that contains GABA, and preparation method thereof, also relate to a kind of brown leben and preparation method thereof.
Background technology
GABA (γ-amino butyric acid is called for short GABA) is a kind of natural functional nonprotein amino acid, extensively is present in the various animal and plant bodies such as tea, radish leaves, shell-fish, mammal.GABA in the human body is not only passed on material as inhibiting nerve, and high concentration is present in the central nervous system, also is present in the tip internal organs such as enteron aisle.GABA is a kind of physiologically active ingredient; Have activate glucose metabolism in the brain, fall blood ammonia, anticonvulsion, hypotensive, promote the brain vigor, increase neurotrophy, strengthen long-term memory, promote growth hormone secretion, activation renal function, liver function, improve important physiological function such as climacteric metancholia.GABA has good medical applications prospect; Be again a kind of novel foodstuff active factors simultaneously, the application in functional food has become the research focus.
Lactic acid bacteria can the enhances human body immunologic function, improves nutritive value of food.Discover that the various lactobacillus strain all has glutamate decarboxylase, can become GABA with glutamic acid rotating.But these bacterial strains have plenty of the bacterial strain that national regulation can not use in dairy products; The poor performance of the fermented milk that has can only be grown in the culture medium of high nutrition such as MRS; The ability of the product GABA that has is low excessively.This has all limited the development and the application of being rich in the GABA dairy products.
Brown stain probio milk beverage is through high-temperature heat treatment; Make between the carbonyls such as amino-compound and sugar in the cow's milk Maillard reaction takes place; Adopt lactic acid bacteria through lengthy fermentation; Again with sterilization after white granulated sugar, aqueous thickener solution proportioning after, it is clearly good to eat that technologies such as acid adjustment, homogeneous, cooling, can are processed, the milk beverage of unique flavor.In Japan, the U.S. and Taiwan, industrialization product is more, is representative especially with the chlorella yakult at present, and its whole world sales volume every day has reached 2500t.Domestic part dairy products enterprise has successively released brown probio milk beverage, and output is soaring year by year, can predict its good market prospects.Need pass through long-time sterilization when generally preparing the brown milk beverage, generally need make newborn base-material fully carry out brown stain at 90~95 ℃ of following sterilization 180min, lengthy fermentation is general more than 3 days then.Long-time sterilization process energy consumption is higher, and the hold facility time is long.
Summary of the invention
Technical problem to be solved by this invention is that sterilization time is long when having overcome prior art production brown milk beverage; Hold facility is high; Use GABA to need that outer to add cost too high in the food, the bacterial classification that perhaps produces GABA in being applied to food the time output low excessively, even can not be applied to the defective in the food; A kind of brown acidified milk base-material that contains GABA and preparation method thereof is provided, and a kind of brown leben that contains GABA and preparation method thereof.This contains in the brown acidified milk base-material of GABA and is rich in GABA, has shortened sterilization time in its preparation method greatly.The brown leben product mouthfeel, the excellent flavor that use this brown acidified milk base-material that contains GABA to make, and contain a large amount of GABAs.
The present invention solves the problems of the technologies described above through following technical scheme.
The invention provides a kind of preparation method who contains the brown acidified milk base-material of GABA, it comprises the steps:
(1) raw material A is mixed, homogeneous gets feed liquid B; Wherein, described raw material A comprises: monose, sodium glutamate and raw milk; Contain the sodium glutamate of 7~10g/L and the monose of 60~150g/L among the described feed liquid B;
(2) described feed liquid B carries out Maillard reaction simultaneously in 90~95 ℃ of following sterilization 60~120min or in 115~120 ℃ of following sterilization 10~20min, cooling;
(3) inoculating starter fermentation promptly gets; Wherein, the time of described fermentation is 72h~96h; Described leavening satisfies following condition: in raw milk, add the 10g/L sodium glutamate, with 1 * 10 6The inoculum concentration inoculating starter of cfu/mL, the output>=4g/L of GABA behind the fermentation 72h.
In the step (1), described raw milk is the conventional raw milk of using in this area, can be rich milk, skimmed milk or low fat milk.The kind of described raw milk is the conventional raw milk of using in this area, generally is selected from cow's milk, goat breast, newborn, the hunchbacked breast of sheep and horse breast.Wherein, described rich milk does not pass through any ungrease treatment.Described skimmed milk is through ungrease treatment.Here, described ungrease treatment is the defatting technology that the technical staff in present technique field knows.Described raw milk can be sweet milk or reconstituted milk.Described reconstituted milk is generally formulated by milk powder and water.For example, described reconstituted milk can be by the preparation of 80~130 weight portion milk powder and 920~870 weight parts waters, preferably after preparation in 4 ℃ of stirring at low speed hydration 6h.
In the step (1), described monose is the conventional glucide that uses in this area.Described monose preferably is fructose and/or glucose.
In the step (1), said method of mixing and condition are this area conventional method and condition.The temperature of said mixing preferably is 40~50 ℃.The time of said mixing is as the criterion to mix, and preferably is 30~40min.
In the step (1), the method for said homogeneous and condition are this area conventional method and condition.The temperature of said homogeneous preferably is 55~75 ℃.The pressure of said homogeneous preferably is 18~25MPa.Described homogeneous preferably is a double-stage homogenization.In the described double-stage homogenization, the one-level homogenization pressure preferably is 15~17MPa, more preferably is 16.5MPa; In the described double-stage homogenization, double-stage homogenization pressure preferably is 3~8MPa, more preferably is 3.5MPa.
In the step (2), when said sterilization, among the described feed liquid B Maillard reaction takes place, brown stain takes place.
In the step (2), the method for said cooling and condition are this area conventional method and condition.The temperature of said cooling preferably is 30~42 ℃.Described cooling is preferably carried out through pipeline plate or interlayer cylinder.
In the step (3), the temperature of said fermentation is the conventional fermentation temperature in this area, preferably is 30~42 ℃.The inoculum concentration of said leavening is the conventional inoculum concentration of using in this area, preferably is 1 * 10 6~1 * 10 7Cfu/mL.
Among the present invention, the bacterial classification of described leavening is country allows use in food bacterial classification (announcing 2011 No. 1 bulletin of " the bacterial classification list that can be used for food " and the Ministry of Public Health by the Ministry of Public Health on April 22nd, 2010), and is safe.Wherein, said leavening satisfies following condition: in raw milk, add the 10g/L sodium glutamate with 1 * 10 6The inoculum concentration inoculating starter of cfu/mL, the output>=4g/L of mensuration GABA behind the fermentation 72h.Those skilled in the art such as the actual conditions of described fermentation such as temperature all know according to the fermentation condition of concrete bacterial classification and suitably adjust.Described leavening preferably is one or more among the leavening choozit Lh100 of leavening Yo-mix 883 and Danisco US Inc. Genencor Divisi of leavening ABT-5, Danisco (Danisco) Co., Ltd of Ke Hansen (Chr Hansen) Co., Ltd.Wherein, described leavening ABT-5 is made up of streptococcus thermophilus (Streptococcus thermophilus), lactobacillus acidophilus (Lactobacillus acidophilus) and bifidobacterium lactis BB-12 (Bifidobacterium lactis BB-12); Described leavening Yo-mix 883 is made up of lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus) and streptococcus thermophilus (Streptococcus thermophilus); Described leavening choozit Lh100 is made up of lactobacillus bulgaricus (Lactobacillus bulgaricus) and Lactobacillus helveticus (Lactobacillus helveticus).
The present invention also provides a kind of brown acidified milk base-material that contains GABA that is made by above-mentioned preparation method.
Among the present invention, the said brown acidified milk base-material that contains GABA can be put in storage by this area conventional method after preparation, and is for use in 2~6 ℃ of storages.
The present invention also provides a kind of preparation method of brown leben, and it comprises the steps:
1. raw material C is mixed, sterilization, cooling gets feed liquid D;
2. described feed liquid D and the said brown acidified milk base-material that contains GABA are mixed, acid adjustment, homogeneous promptly gets;
Perhaps, it comprises the steps:
1. ' raw material C and the said brown acidified milk base-material that contains GABA are mixed acid adjustment, homogeneous;
2. ' sterilization, cooling promptly gets;
Wherein, described raw material C comprises sweetener and water.
Among the present invention; Described sweetener can be the conventional various sweeteners that use in this area, preferably is in sucrose, beet sugar, maltose, glucose, fructose, lactose, HFCS, glycitols sweetener, Sucralose, acesulfame potassium, Aspartame and the honey element one or more.The consumption of said sweetener is the conventional amount used of this area, the consumption when preferably being the sweetness of cane sugar of amounting to into 70~90g sucrose/L raw material C.
Among the present invention, described raw material C preferably also comprises one or more in thickener, nutrition fortifier, flavor substance and the Juice.Described thickener can be the conventional thickener that uses in this area, preferably is in pectin, soybean polyoses and the sodium carboxymethylcellulose one or more.The consumption of said thickener is the conventional amount used of this area, preferably is 1.5~3.5g/L.Wherein, the consumption of said pectin preferably is 2.5~3.5g/L.The consumption of said soybean polyoses preferably is 1.5~2.5g/L.The consumption of said sodium carboxymethylcellulose preferably is 1.5~2.5g/L.Described nutrition fortifier can be the conventional nutrition fortifier that uses in this area, like vitamin and/or calcium-nutrition intensifying agent etc.Described flavor substance can be the conventional flavor substance that uses in this area, like various essence.Described Juice can be the conventional Juice that uses in this area.The consumption of said nutrition fortifier, flavor substance and Juice all can be the conventional amount used of those materials of this area.Described g/L is the ratio of the quality of said thickener with respect to said raw material C cumulative volume.
Step 1. in, said method of mixing and condition are conventional method and condition of this area.The temperature of said mixing preferably is 70~75 ℃.The time of said mixing is as the criterion to mix, and preferably is 30~40min.
Step 1. in, the method for said sterilization and condition are conventional method and condition of this area.The temperature of said sterilization preferably is 90~95 ℃.The time of said sterilization preferably is 5~10min.
Step 1. in, the method for said cooling and condition are conventional method and condition of this area.The temperature of said cooling preferably is 20~40 ℃.
Step 2. in, said feed liquid D and the said mass ratio that contains the brown acidified milk base-material of GABA can be the conventional amount ratio in this area, preferably are 55: 45~80: 20.
Step 2. in, said method of mixing and condition are conventional method and condition of this area.The temperature of said mixing preferably is 20~40 ℃.The time of said mixing is as the criterion to mix, and preferably is 30~40min.
Step 2. in, the method for said acid adjustment and condition are conventional method and condition of this area.The acidity of said acid adjustment preferably is 68~72 ° of T.
Step 2. in, the method for said homogeneous and condition are conventional method and condition of this area.The pressure of said homogeneous preferably is 190~210bar.
Among the present invention, adopting step 1. is viable bacteria type brown leben with the brown leben that 2. step makes.
Step is 1. ' in, said raw material C and the said mass ratio that contains the brown acidified milk base-material of GABA can be the conventional amount ratio in this area, preferably are 55: 45~80: 20.
Step is 1. ' in, said method of mixing and condition can be this area conventional method and condition.Said mixing is preferably undertaken by following operation: with described raw material C premixed, mix with the said brown acidified milk base-material that contains GABA after the cooling again again.Wherein, said premixed temperature preferably is 70~75 ℃; The said premixed time is as the criterion to mix, and preferably is 30~40min; The method of said cooling and condition are conventional method and condition of this area, and the temperature of said cooling preferably is 20~40 ℃; Said decomposite temperature preferably is 20~40 ℃; The said decomposite time is as the criterion to mix, and preferably is 30~40min.
Step is 1. ' in, the method for said acid adjustment and condition are conventional method and condition of this area.The acidity of said acid adjustment preferably is 68~72 ° of T.
Step is 1. ' in, the method for said homogeneous and condition are conventional method and condition of this area.The pressure of said homogeneous preferably is 190~210bar.
Step is 2. ' in, the method for said sterilization and condition are conventional method and condition of this area.The temperature of said sterilization preferably is 120~140 ℃.The time of said sterilization preferably is 3~10s.
Step is 2. ' in, the method for said cooling and condition are conventional method and condition of this area.The temperature of said cooling preferably is 20~40 ℃.
' and step 2. ' brown leben of making is non-viable bacteria type brown leben among the present invention, to adopt step 1..
The present invention also provides a kind of brown leben that is made by above-mentioned preparation method.
Wherein, adopting step 1. is viable bacteria type brown leben with the brown leben that 2. step makes, and its conventional method by viable bacteria type milk beverage is preserved, and needs 4~10 ℃ of refrigerations.
' and step 2. ' brown leben of making is non-viable bacteria type brown leben wherein, to adopt step 1., and its conventional method by non-viable bacteria type milk beverage is preserved, and storage at normal temperature gets final product.
Device therefor is the conventional equipment in this area among the preparation method of the present invention, and related process all can be operated according to routine techniques in this area and normal condition except that specifying.
On the basis that meets this area general knowledge, above-mentioned each optimum condition, but combination in any promptly get each preferred embodiments of the present invention.
Agents useful for same of the present invention and raw material are all commercially available to be got.
Positive progressive effect of the present invention is:
(1) the preparation method's material generation Maillard reaction when sterilization that contains the brown acidified milk base-material of GABA of the present invention; Generate the flavor substance of brown; Add sodium glutamate and can shorten sterilization time, increase utilization rate of equipment and installations, cut down the consumption of energy; Sodium glutamate consumption is complete when fermentation ends, do not influence the mouthfeel and the local flavor of product fully.The brown leben product mouthfeel, the excellent flavor that use this brown acidified milk base-material that contains GABA to make, and contain a large amount of GABAs.This local flavor that has not only increased acidified milk has improved consumer's appetite again.So yogurt drink of the present invention not only has the local flavor of brown milk beverage but also have the functional of GABA, can satisfy the demands of consumers better.
(2) filter out the higher bacterial classification of production GABA ability from the commercially available lactobacillus inoculation that is used for food industry; This bacterial strain is applied in the acidified milk; Develop natural fermented and be rich in the functional dairy product of GABA; Increased the functional and health of dairy products, it is higher to have remedied natural GABA price, the situation of limited use.
The specific embodiment
Mode through embodiment further specifies the present invention below, but does not therefore limit the present invention among the described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example according to conventional method and condition, or is selected according to catalogue.
Stirring, homogeneous, sterilization, filling apparatus: APV company produces, bright dairy industry dairy products eight factories all.
Among the following embodiment, used leavening satisfies following condition: in raw milk, add the 10g/L sodium glutamate with 1 * 10 6The inoculum concentration inoculating starter of cfu/mL, the output>=4g/L of GABA behind the fermentation 72h.
Among the following embodiment, fresh milk originates from Shanghai Bright Dairy & Food Co., Ltd., and wherein protein 3.0%, fat 3.1%.Skimmed milk power is available from the permanent natural group of New Zealand, and wherein protein 34%.Leavening ABT-5 is available from Hansen Corp. of section.Leavening Yo-mix 883 and leavening choozit Lh100 are available from Danisco A/S BJ Rep Office.
Embodiment 1:
1, the preparation method who contains the brown acidified milk base-material of GABA:
(1) fresh milk, glucose and sodium glutamate are mixed 30min to mixing in 45 ℃; Under 60 ℃, carry out double-stage homogenization, wherein the one-level homogenization pressure is 15MPa, and double-stage homogenization pressure is 5MPa; Get feed liquid B, contain the glucose of 150g/L and the sodium glutamate of 7g/L among this feed liquid B;
(2) this feed liquid B carries out Maillard reaction simultaneously in 90~95 ℃ of following sterilization 60min, is cooled to 32 ℃;
(3) inoculating starter ABT-5, inoculum concentration is 1 * 10 6Cfu/mL in 32 ℃ of fermentation 72h, promptly gets the brown acidified milk base-material that contains GABA 4~5g/L.
2, the preparation method of brown leben:
1. pectin, white granulated sugar and water are mixed 30min to mixing under 70 ℃,, be cooled to 30 ℃, get feed liquid D, contain the pectin of 3g/L and the white granulated sugar of 90g/L among this feed liquid D in 95 ℃ of following sterilization 5min;
2. this feed liquid of 550g D and the brown acidified milk base-material 450g that contains GABA among the embodiment 1 are mixed 30 minutes to mixing under 30 ℃, add citric acid acid adjustment to 68 ° T, the 190bar homogeneous promptly gets.
This brown leben is a viable bacteria type leben, and the content of GABA is 2.0~2.5g/L in the finished product.
Embodiment 2:
1, the preparation method who contains the brown acidified milk base-material of GABA:
(1) skimmed milk power and the water mass ratio by 1: 7 is restored; Reconstituted milk, fructose and sodium glutamate are mixed 40min to mixing in 40 ℃; Under 65 ℃, carry out double-stage homogenization, wherein the one-level homogenization pressure is 17MPa, and double-stage homogenization pressure is 3MPa; Get feed liquid B, contain the fructose of 60g/L and the sodium glutamate of 10g/L among this feed liquid B;
(2) this feed liquid B carries out Maillard reaction simultaneously in 115 ℃ of following sterilization 10min, is cooled to 42 ℃;
(3) inoculating starter choozit Lh100, inoculum concentration is 1 * 10 7Cfu/mL in 42 ℃ of fermentation 96h, promptly gets the brown acidified milk base-material that contains GABA 5~6g/L.
2, the preparation method of brown leben:
1. ' with raw material C: white granulated sugar, water, sodium carboxymethylcellulose are total to 700g in 70 ℃ of premixed 35min; Be cooled to 35 ℃; The brown acidified milk base-material 300g that contains GABA with embodiment 2 mixes 40min again to mixing 72 ° of T of acid adjustment, 200bar homogeneous again; Contain the white granulated sugar of 70g/L and the sodium carboxymethylcellulose of 2g/L among this raw material C;
2. ' in 135 ℃ of following sterilization 5s, be cooled to 25 ℃, promptly get.
This brown leben is non-viable bacteria type leben, and the content of the GABA in the finished product is 1.5~1.8g/L.
Embodiment 3:
1, the preparation method who contains the brown acidified milk base-material of GABA:
(1) fresh milk, glucose and sodium glutamate are mixed 35min to mixing in 50 ℃; Under 60 ℃, carry out double-stage homogenization, wherein the one-level homogenization pressure is 17MPa, and double-stage homogenization pressure is 8MPa; Get feed liquid B, contain the glucose of 100g/L and the sodium glutamate of 8g/L among this feed liquid B;
(2) this feed liquid B carries out Maillard reaction simultaneously in 95 ℃ of following sterilization 120min, is cooled to 30 ℃;
(3) inoculating starter Yo-mix 883, and inoculum concentration is 1 * 10 6Cfu/rmL in 30 ℃ of fermentation 84h, promptly gets the brown acidified milk base-material that contains GABA 5~6g/L.
2, the preparation method of brown leben:
1. ' with raw material C: white granulated sugar, water, soybean polyoses are total to 800g in 75 ℃ of premixed 35min; Be cooled to 40 ℃; The brown acidified milk base-material 200g that contains GABA with embodiment 3 mixes 30min again to mixing 70 ° of T of acid adjustment, homogeneous under 210bar again; Contain the white granulated sugar of 90g/L and the soybean polyoses of 2g/L among this raw material C;
2. ' in 121 ℃ of sterilization 4s, be cooled to 20 ℃, promptly get.
This brown leben is non-viable bacteria type leben, and the content of the GABA in the finished product is 1g/L~1.2g/L.
Effect embodiment
Contrast groups: preparation brown leben, do not contain sodium glutamate in the raw material of its toffee acidified milk base-material, sterilization time is 60~95min, other preparation conditions and embodiment 1 are identical.Wherein, the sterilization time of contrast groups a is 60min, and the sterilization time of contrast groups b is 95min.
Compare with embodiment 1, the color and luster of the brown leben of contrast groups a is obviously light, and the brown leben color and luster of the color and luster of contrast groups b and embodiment 1 is suitable.The brown leben color even of embodiment 1 is consistent, and tissue is fine and smooth.
The brown leben finished product of embodiment 1 is in good condition after 30 days in 4~10 ℃ of refrigerations, and tissue is fine and smooth, stable in properties, and nothing deposition, flocculation situation take place.

Claims (10)

1. preparation method who contains the brown acidified milk base-material of GABA, it comprises the steps:
(1) raw material A is mixed, homogeneous gets feed liquid B; Wherein, described raw material A comprises: monose, sodium glutamate and raw milk; Contain the sodium glutamate of 7~10g/L and the monose of 60~150g/L among the described feed liquid B;
(2) described feed liquid B carries out Maillard reaction simultaneously in 90~95 ℃ of following sterilization 60~120min or in 115~120 ℃ of following sterilization 10~20min, cooling;
(3) inoculating starter fermentation promptly gets; Wherein, the time of described fermentation is 72h~96h; Described leavening satisfies following condition: in raw milk, add the 10g/L sodium glutamate, with 1 * 10 6The inoculum concentration inoculating starter of cfu/mL, the output>=4g/L of GABA behind the fermentation 72h.
2. preparation method as claimed in claim 1 is characterized in that, in the step (1), described monose is fructose and/or glucose; And/or in the step (1), the temperature of said mixing is 40~50 ℃; The time of said mixing is 30~40min; And/or in the step (1), the temperature of said homogeneous is 55~75 ℃; The pressure of said homogeneous is 18~25MPa; Described homogeneous preferably is a double-stage homogenization; Wherein, in the described double-stage homogenization, the one-level homogenization pressure preferably is 15~17MPa, and double-stage homogenization pressure preferably is 3~8MPa.
3. preparation method as claimed in claim 1 is characterized in that, in the step (2), the temperature of said cooling is 30~42 ℃; And/or in the step (2), described cooling is carried out through pipeline plate or interlayer cylinder; And/or in the step (3), the temperature of said fermentation is 30~42 ℃; And/or in the step (3), the inoculum concentration of said leavening is 1 * 10 6~1 * 10 7Cfu/mL.
4. like each described preparation method of claim 1~3; It is characterized in that; In the step (3), said leavening is one or more among the leavening choozit Lh100 of leavening Yo-mix 883 and Danisco US Inc. Genencor Divisi of leavening ABT-5, Danisco US Inc. Genencor Divisi of Ke Hansen Co., Ltd.
5. brown acidified milk base-material that contains GABA that makes by each described preparation method of claim 1~4.
6. the preparation method of a brown leben, it comprises the steps:
1. raw material C is mixed, sterilization, cooling gets feed liquid D;
2. described feed liquid D and the described brown acidified milk base-material that contains GABA of claim 5 are mixed, acid adjustment, homogeneous promptly gets;
Perhaps, it comprises the steps:
1. ' raw material C and the described brown acidified milk base-material that contains GABA of claim 5 are mixed acid adjustment, homogeneous;
2. ' sterilization, cooling promptly gets;
Wherein, described raw material C comprises sweetener and water.
7. preparation method as claimed in claim 6; It is characterized in that described sweetener is one or more in sucrose, beet sugar, maltose, glucose, fructose, lactose, HFCS, glycitols sweetener, Sucralose, acesulfame potassium, Aspartame and the honey element; And/or, the consumption the when consumption of said sweetener is the sweetness of cane sugar of amounting to into 70~90g sucrose/L raw material C; And/or described raw material C also comprises one or more in thickener, nutrition fortifier, flavor substance and the Juice.
8. preparation method as claimed in claim 7 is characterized in that, described thickener is one or more in pectin, soybean polyoses and the sodium carboxymethylcellulose; And/or the consumption of said thickener is 1.5~3.5g/L; Wherein, the consumption of said pectin preferably is 2.5~3.5g/L; The consumption of said soybean polyoses preferably is 1.5~2.5g/L; The consumption of said sodium carboxymethylcellulose preferably is 1.5~2.5g/L; Described g/L is the ratio of the quality of said thickener with respect to said raw material C cumulative volume.
9. preparation method as claimed in claim 6 is characterized in that, step 1. in, the temperature of said mixing is 70~75 ℃; The time of said mixing is 30~40min; And/or, step 1. in, the temperature of said sterilization is 90~95 ℃; The time of said sterilization is 5~10min; And/or, step 1. in, the temperature of said cooling is 20~40 ℃; And/or, step 2. in, said feed liquid D and the said mass ratio that contains the brown acidified milk base-material of GABA are 55: 45~80: 20; And/or, step 2. in, the temperature of said mixing is 20~40 ℃; The time of said mixing is 30~40min; And/or, step 2. in, the acidity of said acid adjustment is 68~72 ° of T; And/or, step 2. in, the pressure of said homogeneous is 190~210bar;
And/or step is 1. ' in, said raw material C and the said mass ratio that contains the brown acidified milk base-material of GABA are 55: 45~80: 20; And/or step is 1. ' in, said mixing is undertaken by following operation: with described raw material C premixed, mix with the said brown acidified milk base-material that contains GABA after the cooling again again; Wherein, said premixed temperature preferably is 70~75 ℃; The said premixed time preferably is 30~40min; The temperature of said cooling preferably is 20~40 ℃; Said decomposite temperature preferably is 20~40 ℃; The said decomposite time preferably is 30~40min; And/or step is 1. ' in, the acidity of said acid adjustment is 68~72 ° of T; And/or step is 1. ' in, the pressure of said homogeneous is 190~210bar; And/or step is 2. ' in, the temperature of said sterilization is 120~140 ℃; The time of said sterilization is 3~10s; And/or step is 2. ' in, the temperature of said cooling is 20~40 ℃.
10. brown leben that makes like each described preparation method of claim 6~9.
CN 201210099746 2012-04-06 2012-04-06 Gamma-reanal containing brown fermented milk base material, milk beverage and preparation methods thereof Active CN102626138B (en)

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CN103621624A (en) * 2012-08-29 2014-03-12 内蒙古蒙牛乳业(集团)股份有限公司 Brown milk drink and production method thereof
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CN103636784A (en) * 2013-12-13 2014-03-19 光明乳业股份有限公司 Brown Chinese rice wine lactobacillus beverage and preparation method for same
CN103636784B (en) * 2013-12-13 2016-04-27 光明乳业股份有限公司 A kind of brown fermented glutinous rice sour milk beverage and preparation method thereof
CN104663892A (en) * 2015-02-13 2015-06-03 河北新希望天香乳业有限公司 Brown fermented milk base material, brown milk beverage and preparation method of brown fermented milk base material
CN104663892B (en) * 2015-02-13 2017-12-19 河北新希望天香乳业有限公司 Brown fermented milk base-material, brown milk drink and preparation method thereof
CN105594928A (en) * 2015-12-30 2016-05-25 福建舜远农业技术开发有限公司 Process for preparing life-preserving gamma-aminobutyric acid moringa oleifera tea by using chilling stress method
CN108124979A (en) * 2017-12-22 2018-06-08 光明乳业股份有限公司 A kind of method for preparing the fermented beverage containing acid whey and the fermented beverage containing acid whey prepared
CN110301480A (en) * 2018-03-20 2019-10-08 内蒙古蒙牛乳业(集团)股份有限公司 Milk-contained drink and preparation method thereof
CN109479959A (en) * 2019-01-11 2019-03-19 吉林大学 A kind of brown ginseng goat milk acidified milk and preparation method thereof
CN109924472A (en) * 2019-04-08 2019-06-25 济南微生慧生物技术有限公司 The Lactobacillus rhamnosus of one plant of highly producing gamma-aminobutyric acid and its application
CN110283855A (en) * 2019-06-20 2019-09-27 绿雪生物工程(深圳)有限公司 A method of it improving 210202 bacterial strain of streptococcus thermophilus and produces GABA ability

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