CN102621246A - Method for simultaneous determination of contents of six active ingredients in Danhong injection using HPLC-DAD - Google Patents
Method for simultaneous determination of contents of six active ingredients in Danhong injection using HPLC-DAD Download PDFInfo
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Abstract
The invention relates to a method for simultaneous determination of contents of six active ingredients (sodium danshensu, protocatechualdehyde, coffeic acid, rosmarinic acid, salvianolic acid B and salvianolic acid A) in Danhong injection through using an HPLC-DAD (high performance liquid chromatography-diode array detector). The concrete technical scheme of the invention is as follows: preparing a sample solution and a control solution, carrying out gradient elution with an HPLC-DAD method, and measuring the contents of the six active ingredients (sodium danshensu, protocatechualdehyde, coffeic acid, rosmarinic acid, salvianolic acid B and salvianolic acid A) in Danhong. The method provided by the invention is simple, high in sensitivity and good in repeatability, solves the problem that in the existing Danhong injection, only the contents of two active ingredients are controlled, so that the quality issue of the Danhong injection cannot be comprehensively reflected, and provides more scientific basis for scientific evaluation and effective control of the quality of the Danhong injection.
Description
Technical field
The invention belongs to the traditional Chinese medicine quality detection range, specifically is the method that a kind of HPLC-DAD of use measures 6 kinds of active constituent contents in the DANHONG ZHUSHEYE simultaneously.
Background technology
DANHONG ZHUSHEYE is the compound preparation that is extracted by traditional blood-activating and stasis-removing red sage root, safflower, has promoting blood circulation and removing blood stasisly, improves microcirculation; Reduce blood viscosity, accelerate velocity of blood flow, remove oxygen radical; The protection cell is avoided oxidative damage, suppresses effects such as atherosclerotic.Be widely used in coronary heart disease, angina pectoris, myocardial infarction, acute cerebral infarction, chronic cardiopulmonary disease, Buerger's disease, atrophic gastritis and hypertensive nephropathy etc. clinically.The monarch drug in a prescription red sage root of DANHONG ZHUSHEYE mainly contains water soluble ingredients such as danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B, salviandic acid A.Only Sodium Danshensu and 2 kinds of effective constituents of protocatechualdehyde are carried out quality control in the national drug standards (trying) the promulgation part about DANHONG ZHUSHEYE in " national standard for traditional Chinese medicines compilation, internal medicine, feel concerned about fascicle ".Because ingredient more complicated in the DANHONG ZHUSHEYE is difficult to 6 kinds of effective constituents are well separated with other compositions, reaches the requirement of HPLC quantitative measurement.In view of this reason, have important practical significance so set up a kind of method of measuring 6 kinds of active constituent contents in the DANHONG ZHUSHEYE simultaneously.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides the method that a kind of HPLC-DAD of use measures 6 kinds of active constituent contents in the DANHONG ZHUSHEYE simultaneously.Can effectively solve with danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B, salviandic acid A as index property composition; Carry out assay simultaneously; Be the problem that the DANHONG ZHUSHEYE quality standard lays the foundation, realize that concrete technical scheme of the present invention is:
A kind of HPLC-DAD of use measures the method for 6 kinds of active constituent contents in the DANHONG ZHUSHEYE simultaneously, it is characterized in that comprising the steps:
1) the accurate DANHONG ZHUSHEYE of drawing adds the ultrapure water dilution, shakes up, and filters through filter membrane, promptly gets need testing solution;
2) precision takes by weighing reference substance Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B, salviandic acid A, puts respectively in the volumetric flask, adds the ultrapure water dissolving, dilution, and mixing, as the reference substance stock solution, refrigeration is subsequent use in 4 ℃ of refrigerators;
3) distinguish the accurate 6 kinds of reference substance stock solutions of drawing again, add the ultrapure water dilution, accurately prepare the mixing reference substance solution of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B, salviandic acid A;
4) carrying out the HPLC-DAD method with following chromatographic condition detects: chromatographic column: Eclipse XDB-C
184.6 * 150 mm, 5 μ m; Moving phase comprises mobile phase A: acetonitrile and Mobile phase B: the phosphate aqueous solution of volumn concentration 0.05%-0.1%; Adopt the gradient elution mode, the volume ratio that acetonitrile accounts for moving phase increases along with the increase of time in following ranges: 0-6 min, 5%-5%; 6-16 min, 5%-15%; 16-30 min, 15%-26%; 35-40 min, 26%-27%; The DAD detecting device detects wavelength: detect Sodium Danshensu, protocatechualdehyde, tanshin polyphenolic acid B, salviandic acid A use 270-290 nm, detect caffeic acid, Rosmarinic acid use 320-340nm; Column temperature: 25-35 ℃; Flow velocity: 0.7-1.2 mLmin
-1
5) get the mixing reference substance solution, with the different multiple of ultrapure water dilution, get above-mentioned diluted mixture reference substance solution and former each the sample introduction 10 μ l of reference substance solution that mix respectively; Record chromatogram and peak area; Peak area Y with 6 kinds of effective constituent chromatographic peaks is an ordinate respectively, with concentration X, and μ gmL
-1For horizontal ordinate carries out linear regression, get the regression equation and the related coefficient of typical curve;
6) get the DANHONG ZHUSHEYE of different lot numbers respectively; Preparation method by need testing solution prepares sample solution; Difference sample introduction 10 μ L; Measure peak area, every lot sample article are measured 3 times, calculate the content of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and salviandic acid A in the different lot number DANHONG ZHUSHEYEs.
Described method is characterized in that the accurate DANHONG ZHUSHEYE 2mL that draws in the step 1), puts in the 10mL volumetric flask, adds ultrapure water and is diluted to scale, shakes up, and filters through 0.45 μ m filter membrane, promptly gets need testing solution.
Described method; It is characterized in that step 2) in precision take by weighing reference substance Sodium Danshensu 6.55mg, protocatechualdehyde 5.05mg, caffeic acid 1.55mg, Rosmarinic acid 5.45mg, tanshin polyphenolic acid B 10.95mg, salviandic acid A 5.60mg, put respectively in the 5mL volumetric flask, add ultrapure water dissolving; Be diluted to scale; Mixing, as the reference substance stock solution, refrigeration is subsequent use in 4 ℃ of refrigerators.
Described method is characterized in that distinguishing accurate a certain amount of 6 kinds of reference substance stock solutions of drawing in the step 3) again, adds the ultrapure water dilution, accurately prepares Sodium Danshensu 1.310 mgmL
-1, protocatechualdehyde 0.101 mgmL
-1, caffeic acid 0.0041 mgmL
-1, Rosmarinic acid 0.109 mgmL
-1, tanshin polyphenolic acid B 0.219 mgmL
-1, salviandic acid A 3.360 mgmL
-1The mixing reference substance solution.
Described method, the volumn concentration that it is characterized in that Mobile phase B phosphate aqueous solution in the step 4) is 0.1%.
Described method is characterized in that the DAD detecting device detects wavelength in the step 4): detection Sodium Danshensu, protocatechualdehyde, tanshin polyphenolic acid B, salviandic acid A use 280 nm, detect caffeic acid, Rosmarinic acid use 330nm.
Described method is characterized in that column temperature is 30 ℃ in the step 4).
Described method is characterized in that flow velocity is 1.0 mLmin in the step 4)
-1
Described method is characterized in that getting in the step 5) mixing reference substance solution, respectively with ultrapure water dilution 1.25; 5,10,20; 100 times, get above-mentioned 5 kinds of diluted mixture reference substance solution and the former reference substance solution sample introduction 10 μ l separately that mix, record chromatogram and peak area; Peak area Y with 6 kinds of effective constituent chromatographic peaks is an ordinate respectively, with concentration X, and μ gmL
-1For horizontal ordinate carries out linear regression, get the regression equation and the related coefficient of typical curve.
The present invention adopts the HPLC-DAD method, and the utilization gradient elution has been measured Sodium Danshensu in the DANHONG ZHUSHEYE, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and 6 kinds of content of effective of salviandic acid A.Calculate the linear relationship of 6 kinds of effective constituents in the DANHONG ZHUSHEYE, the result sees table 1.
The linear relationship of 6 kinds of effective constituents of table 1 (
n=6)
Method of the present invention is easy, highly sensitive; Favorable reproducibility; The quality assessment that has solved present DANHONG ZHUSHEYE can not reflect the problem of DANHONG ZHUSHEYE quality all sidedly; So that the quality of DANHONG ZHUSHEYE is carried out the control of many indexs, for scientific evaluation and the quality of effectively controlling DANHONG ZHUSHEYE provide more scientific basis.
Description of drawings
Fig. 1 is the ultraviolet-visible absorption spectroscopy figure of Sodium Danshensu reference substance;
Fig. 2 is the ultraviolet-visible absorption spectroscopy figure of protocatechualdehyde reference substance;
Fig. 3 is the ultraviolet-visible absorption spectroscopy figure of caffeic acid reference substance;
Fig. 4 is the ultraviolet-visible absorption spectroscopy figure of Rosmarinic acid reference substance;
Fig. 5 is the ultraviolet-visible absorption spectroscopy figure of tanshin polyphenolic acid B reference substance;
Fig. 6 is the ultraviolet-visible absorption spectroscopy figure of salviandic acid A reference substance;
Fig. 7 mixes the chromatogram of reference substance solution at 280nm;
Fig. 8 mixes the chromatogram of reference substance solution at 330nm;
Fig. 9 is the chromatogram of need testing solution (No.110934) at 280nm;
Figure 10 is the chromatogram of need testing solution (No.110934) at 330nm;
Among Fig. 7 to Figure 10,1. Sodium Danshensu; 2. protocatechualdehyde; 3. caffeic acid; 4. Rosmarinic acid; 5.
Tanshin polyphenolic acid B; 6. salviandic acid A.
Embodiment
Material
Agilent 1200 type highly effective liquid phase chromatographic systems (comprising G1311A quaternary gradient pump, G1316A column oven, G1315D PDAD, G1315D DAD detecting device, the online degasser of G1322A and chem workstation).Ultrapure water appearance (U.S. Milipore company); XS105DualRange analytical balance (plum Teller-Tuo benefit Instr Ltd.); Ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.).
DANHONG ZHUSHEYE (Heze Buchang Pharma Co., Ltd., specification 10 mL/ prop up); Sodium Danshensu reference substance (lot number 110855-200809), protocatechualdehyde reference substance (lot number 110810-201007), caffeic acid reference substance (lot number 20120211), Rosmarinic acid reference substance (lot number 111871-201102) are purchased in Nat'l Pharmaceutical & Biological Products Control Institute; Tanshin polyphenolic acid B reference substance (lot number 20110822), salviandic acid A reference substance (lot number 20120207) are purchased the leaf bio tech ltd in the source, Shanghai.Acetonitrile (lot number 102489), methyl alcohol (lot number 101437) are purchased the Fisher company in the U.S., are chromatographically pure; Phosphoric acid, (lot number 76643827, chemistry company limited of traditional Chinese medicines group) is pure for analyzing; Water is ultrapure water.
Method and result
2.1Accurate DANHONG ZHUSHEYE 2 mL that draw of the preparation of need testing solution put in the 10 mL volumetric flasks, add ultrapure water and are diluted to scale, shake up, and filter through 0.45 μ m filter membrane, promptly get need testing solution.
Mix the preparation of reference substance solution: precision takes by weighing reference substance Sodium Danshensu 6.55 mg, protocatechualdehyde 5.05 mg, caffeic acid 1.55 mg, Rosmarinic acid 5.45 mg, tanshin polyphenolic acid B 10.95 mg, salviandic acid A 5.60 mg; Put respectively in the 5 mL volumetric flasks; Add the ultrapure water dissolving, be diluted to scale, mixing; As the reference substance stock solution, refrigeration is subsequent use in 4 ℃ of refrigerators.
A certain amount of 6 kinds of reference substance stock solutions of accurate respectively absorption add the ultrapure water dilution again, accurately prepare Sodium Danshensu 1.310 mgmL
-1, protocatechualdehyde 0.101 mgmL
-1, caffeic acid 0.0041 mgmL
-1, Rosmarinic acid 0.109 mgmL
-1, tanshin polyphenolic acid B 0.219 mgmL
-1, salviandic acid A 3.360 mgmL
-1The mixing reference substance solution.
Chromatographic conditionEclipse XDB-C
18Chromatographic column (4.6 * 150 mm, 5 μ m); Moving phase adopts binary system, and A is an acetonitrile, and B is 0.1% phosphate aqueous solution, and the gradient elution mode is 0-6 min, 5%A; 6-16 min, 5%A-15%A; 16-30 min, 15%A-26%A; 30-40 min, 26%A-27%A; Column temperature is 30 ℃; Flow velocity is 1.0 mLmin
-1The result obtains the ultraviolet-visible spectrum like 6 kinds of reference substances of Fig. 1-shown in Figure 6; Confirming to detect wavelength by Fig. 1 and need testing solution chromatogram is 280 nm (measuring Sodium Danshensu, protocatechualdehyde, tanshin polyphenolic acid B, salviandic acid A), 330 nm (measuring caffeic acid, Rosmarinic acid).Under selected chromatographic condition, 6 kinds of compositions are well separated with other coexistence peaks in the sample liquid, mix the chromatogram of reference substance solution and need testing solution and see Fig. 7-Figure 10.
2.4 investigating, linear relationship gets the mixing reference substance solution, respectively with ultrapure water dilution 1.25,5,10,20,100 times.Get above-mentioned 5 kinds of diluted mixture reference substance solution and former each the sample introduction 10 μ l of reference substance solution that mix, record chromatogram and peak area, the peak area (Y) with 6 kinds of effective constituent chromatographic peaks is an ordinate respectively, with concentration (X, μ gmL
-1) for horizontal ordinate carries out linear regression, getting the regression equation and the related coefficient of typical curve, the result sees table 1.
The linear relationship of 6 kinds of effective constituents of table 1 (
n=6)
2.5 the mixing reference substance solution is got in the precision test, by above-mentioned chromatographic condition, and difference sample introduction 10 μ l, continuous sample introduction is measured 6 times, and the RSD that calculates 6 kinds of reference substance peak areas is respectively 0.14%, 0.24%, 0.06%, 0.08%, 0.06%, 0.23%.
2.6 replica test is got 6 parts of same lot number DANHONG ZHUSHEYEs (No.110928); Press 6 parts of test liquids of the parallel preparation of preparation method of need testing solution; With above-mentioned chromatographic condition analysis, every duplicate samples sample introduction 3 times, sample size 10 μ l; Record chromatogram and peak area, the RSD of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and salviandic acid A content is respectively 0.06%, 0.24%, 0.35%, 0.14%, 0.43%, 1.53% in 6 parts of test liquids as a result.
2.7 same need testing solution solution (No.110928) is got in stability test; 0,2,4,8,16,24 h sample introductions, 10 μ L after preparing respectively; Measure peak area; The RSD of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and salviandic acid A peak area is respectively 0.18%, 0.28%, 0.16%, 0.78%, 0.77%, 0.24% as a result, shows that need testing solution is good at 24 h internal stabilities.
2.8 accurate 6 parts of each 1 mL of DANHONG ZHUSHEYE (lot number 110928) that draw known content of average recovery test; Place 10 mL volumetric flasks respectively; Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and the salviandic acid A reference substance of accurate adding 100%; 6 parts of need testing solutions of parallel preparation; By above-mentioned chromatographic condition; Each need testing solution sample introduction 10 μ L, the peak area of 6 kinds of effective constituents of mensuration, the average recovery rate that calculates Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and salviandic acid A is respectively 98.98 ± 1.17% (RSD=1.19%), 100.86 ± 1.03% (RSD=1.02%), 100.44 ± 1.31% (RSD=1.30%), 98.73 ± 1.80% (RSD=1.82%), 101.35 ± 1.65% (RSD=1.63%), 100.17 ± 1.26% (RSD=1.26%).
2.9 sample size is measured the DANHONG ZHUSHEYE get different lot numbers respectively, prepares sample solution by the preparation method of need testing solution, sample introduction 10 μ L measure peak area respectively, every lot sample article mensuration 3 times.Calculate the content of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and salviandic acid A in the different lot number DANHONG ZHUSHEYEs, the result sees table 2.
6 kinds of content of effective in the table 2 different batches DANHONG ZHUSHEYE (
n
= 3)
Claims (8)
1. a method of using HPLC-DAD to measure 6 kinds of active constituent contents in the DANHONG ZHUSHEYE simultaneously is characterized in that comprising the steps:
1) the accurate DANHONG ZHUSHEYE of drawing adds the ultrapure water dilution, shakes up, and filters through filter membrane, promptly gets need testing solution;
2) precision takes by weighing reference substance Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B, salviandic acid A, puts respectively in the volumetric flask, adds the ultrapure water dissolving, dilution, and mixing, as the reference substance stock solution, refrigeration is subsequent use in 4 ℃ of refrigerators;
3) distinguish the accurate 6 kinds of reference substance stock solutions of drawing again, add the ultrapure water dilution, accurately prepare the mixing reference substance solution of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B, salviandic acid A;
4) carrying out the HPLC-DAD method with following chromatographic condition detects: chromatographic column: Eclipse XDB-C
184.6 * 150 mm, 5 μ m; Moving phase comprises mobile phase A: acetonitrile and Mobile phase B: the phosphate aqueous solution of 0.05%-0.1% (volumn concentration); Adopt the gradient elution mode, the percent by volume that acetonitrile accounts for moving phase increases along with the increase of time in following ranges: 0-6 min, 5%-5%; 6-16 min, 5%-15%; 16-30 min, 15%-26%; 35-40 min, 26%-27%; The DAD detecting device detects wavelength: detect Sodium Danshensu, protocatechualdehyde, tanshin polyphenolic acid B, salviandic acid A use 270-290 nm, detect caffeic acid, Rosmarinic acid use 320-340nm; Column temperature: 25-35 ℃, preferred 30 ℃; Flow velocity: 0.7-1.2 mLmin
-1
5) get the mixing reference substance solution, with the different multiple of ultrapure water dilution, get above-mentioned diluted mixture reference substance solution and former each the sample introduction 10 μ l of reference substance solution that mix respectively; Record chromatogram and peak area; Peak area Y with 6 kinds of effective constituent chromatographic peaks is an ordinate respectively, with concentration X, and μ gmL
-1For horizontal ordinate carries out linear regression, get the regression equation and the related coefficient of typical curve;
6) get the DANHONG ZHUSHEYE of different lot numbers respectively; Preparation method by need testing solution prepares sample solution; Difference sample introduction 10 μ L; Measure peak area, every lot sample article are measured 3 times, calculate the content of Sodium Danshensu, protocatechualdehyde, caffeic acid, Rosmarinic acid, tanshin polyphenolic acid B and salviandic acid A in the different lot number DANHONG ZHUSHEYEs.
2. the method for claim 1 is characterized in that the accurate DANHONG ZHUSHEYE 2mL that draws in the step 1), puts in the 10mL volumetric flask, adds ultrapure water and is diluted to scale, shakes up, and filters through 0.45 μ m filter membrane, promptly gets need testing solution.
3. the method for claim 1; It is characterized in that step 2) in precision take by weighing reference substance Sodium Danshensu 6.55mg, protocatechualdehyde 5.05mg, caffeic acid 1.55mg, Rosmarinic acid 5.45mg, tanshin polyphenolic acid B 10.95mg, salviandic acid A 5.60mg, put respectively in the 5mL volumetric flask, add ultrapure water dissolving; Be diluted to scale; Mixing, as the reference substance stock solution, refrigeration is subsequent use in 4 ℃ of refrigerators.
4. the method for claim 1 is characterized in that distinguishing accurate a certain amount of 6 kinds of reference substance stock solutions of drawing in the step 3) again, adds the ultrapure water dilution, accurately prepares Sodium Danshensu 1.310 mgmL
-1, protocatechualdehyde 0.101 mgmL
-1, caffeic acid 0.0041 mgmL
-1, Rosmarinic acid 0.109 mgmL
-1, tanshin polyphenolic acid B 0.219 mgmL
-1, salviandic acid A 3.360 mgmL
-1The mixing reference substance solution.
5. the method for claim 1, the volumn concentration the best that it is characterized in that Mobile phase B phosphate aqueous solution in the step 4) is 0.1%.
6. the method for claim 1 is characterized in that the DAD detecting device detects wavelength in the step 4): detect Sodium Danshensu, protocatechualdehyde, tanshin polyphenolic acid B, salviandic acid A and use about 280 nm, detect caffeic acid, Rosmarinic acid uses about 330nm.
7. the method for claim 1 is characterized in that flow velocity the best is 1.0 mLmin in the step 4)
-1
8. the method for claim 1 is characterized in that getting in the step 5) mixing reference substance solution, respectively with ultrapure water dilution 1.25; 5,10,20; 100 times, get above-mentioned 5 kinds of diluted mixture reference substance solution and former each the sample introduction 10 μ l of reference substance solution that mix, record chromatogram and peak area; Peak area Y with 6 kinds of effective constituent chromatographic peaks is an ordinate respectively, with concentration X, and μ gmL
-1For horizontal ordinate carries out linear regression, get the regression equation and the related coefficient of typical curve.
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| CN103913434A (en) * | 2014-04-04 | 2014-07-09 | 浙江大学 | Online method for detecting water sinking process of danhong injection |
| CN103940929A (en) * | 2014-05-05 | 2014-07-23 | 山东丹红制药有限公司 | Method for detecting traditional Chinese medicine injection for treating cardiovascular and cerebrovascular diseases |
| CN104374844A (en) * | 2014-11-13 | 2015-02-25 | 贵州信邦制药股份有限公司 | Method for detecting content of water-soluble ingredients in salvia miltiorrhiza medicinal material and application of method |
| CN108037193A (en) * | 2017-11-01 | 2018-05-15 | 广西壮族自治区食品药品检验所 | Using the method for HPLC-UV-ELSD methods detection Fufang Danshen Pian |
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| CN114295738A (en) * | 2021-12-08 | 2022-04-08 | 江苏弘典中药产业研究院有限公司 | Quality detection method of salvia miltiorrhiza leaf tea |
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| CN103913434A (en) * | 2014-04-04 | 2014-07-09 | 浙江大学 | Online method for detecting water sinking process of danhong injection |
| CN103940929A (en) * | 2014-05-05 | 2014-07-23 | 山东丹红制药有限公司 | Method for detecting traditional Chinese medicine injection for treating cardiovascular and cerebrovascular diseases |
| CN103940929B (en) * | 2014-05-05 | 2015-11-04 | 山东丹红制药有限公司 | A kind of detection method for the treatment of the traditional Chinese medicine injection of cardiovascular and cerebrovascular disease |
| CN104374844A (en) * | 2014-11-13 | 2015-02-25 | 贵州信邦制药股份有限公司 | Method for detecting content of water-soluble ingredients in salvia miltiorrhiza medicinal material and application of method |
| CN108037193A (en) * | 2017-11-01 | 2018-05-15 | 广西壮族自治区食品药品检验所 | Using the method for HPLC-UV-ELSD methods detection Fufang Danshen Pian |
| CN108037193B (en) * | 2017-11-01 | 2020-08-04 | 广西壮族自治区食品药品检验所 | Method for detecting compound salvia miltiorrhiza tablets by adopting HP L C-UV-E L SD method |
| CN110632190A (en) * | 2019-08-28 | 2019-12-31 | 湖南先伟实业有限公司 | Method for simultaneously measuring rosmarinic acid, carnosic acid and carnosol in rosemary |
| CN114295738A (en) * | 2021-12-08 | 2022-04-08 | 江苏弘典中药产业研究院有限公司 | Quality detection method of salvia miltiorrhiza leaf tea |
| CN114295738B (en) * | 2021-12-08 | 2024-04-02 | 江苏弘典中药产业研究院有限公司 | Quality detection method of red sage leaf tea |
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