CN108828120A - A method of honeysuckle active component is measured using HPLC method - Google Patents
A method of honeysuckle active component is measured using HPLC method Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention discloses a kind of methods using HPLC method measurement honeysuckle active component, include the following steps：Under chromatographic condition, specificity test, linear relationship investigation, precision test, repetitive test, stability test and sample recovery rate test are carried out respectively；The chromatographic condition is：Detector：Diode array detector；Chromatographic column：YMC-Pack ODS-A C18 chromatographic column (150mm × 4.6mm, 5 μm)；Mobile phase：Acetonitrile (A)：0.6% acetic acid (B)；A：8%-28%, 0-30min；B：92%-72%, 0-30min；Detection wavelength：330nm；Flow velocity：1.0mL/min；Column temperature：25℃；Proportionate relationship having the same between each active component in the reference substance solution and sample solution.Method of the invention is combined using high performance liquid chromatography and uv detection method, has detected eight kinds of effective components and its content in 50% methanol extract liquid of honeysuckle；By methodological study, show using method it is good, it is accurate to stablize, it is sensitive efficiently, the rate of recovery is high.
Description
Technical field
The invention belongs to traditional Chinese medicine ingredients detection technique fields.Honeysuckle is measured using HPLC method more particularly, to a kind of
The method of active component.
Background technique
Honeysuckle is by (version in 1963) beam《Mingyi Bielu》It includes, is the dry flower of caprifoliaceae plant, it is drought-enduring resistance to
It is cold.(version in 1977)《Chinese Pharmacopoeia》, selecting Lonicera japonica Thunb., Lonicera confusa DC. etc. is
The former plant of honeysuckle.(version in 2005)《Chinese Pharmacopoeia》Honeysuckle is divided into honeysuckle and Honeysuckle flower etc., and includes a variety of phases
Honeysuckle class plant is closed, determines that the certified products of medicinal honeysuckle is honeysuckle Lonicera japonica Thunb..Honeysuckle
Lonicera japonica Thunb. is high in Chinese yield, and distribution is wide, cheap and easy to get, provides source for subsequent research and protects
Barrier,
So far, the main component for having detected that honeysuckle includes has:Organic acid compound, triterpene soaps chemical combination
Object, flavone compound, inorganic elements class etc., drug effect is mainly manifested in:Antipyretic and anti-inflammatory, resisting pathogenic microbes, anti-oxidant, guarantor
Liver cholagogue, immune, antitumor, the hypoglycemic, reducing blood lipid of raising etc..It wherein include galuteolin, caryophyllus oil in flavone compound
Phenol, geraniol, rutin etc.；Organic acid compound includes the compounds such as chlorogenic acid, protocatechuic acid, caffeic acid and isochlorogenic acid
Equal ingredients.Also contain volatile oil, such as myristic acid, benzyl carbinol, palmitinic acid, linoleic acid, linalool, geraniol etc. in honeysuckle
With triterpene soaps such as hederagenin, oleanolic acid, sapindoside, honeysuckle flower saponin(e first [9]；It is total containing inorganic microelement
15 kinds of such as calcium, phosphorus, zinc, copper, barium, iron, manganese.
HPLC (high performance liquid chromatography) method is used widely in terms of quality testing in analysis drug, food.HPLC method
Honeysuckle and its related preparations quality can be efficiently controlled, for the quality-monitoring for improving flos lonicerae composite drug and Chinese patent drug
The guarantee of provider's science of law.Meanwhile a large number of researchers optimize and improve detection method, the foundation reversed phase high performance liquid such as side's passage
Phase chromatography establishes method of quality control that is a kind of while measuring rutin, Quercetin.This method improves detection efficiency, as a result
Favorable reproducibility can be used for the quality-monitoring of honeysuckle.High performance liquid chromatography is detection honeysuckle and its related Chinese medicine composite medicine
The primary analysis method of active constituent content in agent.It is more that researcher generally detects single chemical component type, and for same
When detect that the Comparison between detecting methods of plurality of active ingredients in honeysuckle are rare.
Summary of the invention
In order to solve the above technical problems, being lived it is an object of the present invention to provide a kind of using HPLC method measurement honeysuckle
The method of property component.This method can carry out overall merit to 8 kinds of effective components in honeysuckle and measure each component content, be gold and silver
The Quality Identification of anther material provides foundation.
In order to achieve the above objectives, the present invention uses following technical scheme：
A method of honeysuckle active component is measured using HPLC method, is included the following steps：
1) under chromatographic condition, sample solution and reference substance solution is injected separately into high performance liquid chromatograph and detected,
Compare the separating degree of the adjacent chromatographic peak of two kinds of solution；If the separating degree is greater than 1.5, illustrate each active group in sample solution
/ it is separable；
2) under chromatographic condition, by the reference substance solution of 2.5-25 μ L different volumes be injected separately into high performance liquid chromatograph into
Row detection is drawn with sample volume (μ g) for abscissa (x) by ordinate (y) of each active component chromatographic peak integrating peak areas value
Standard curve, if the R of each active component2It is all larger than 0.9, then illustrates each active component in sample solution in respective range interior lines
Sexual intercourse is good；
3) under chromatographic condition, the reference substance solution injection high performance liquid chromatograph of 10 μ L is detected, continuous sample introduction 6
It is secondary, the chromatographic peak peak area of each active component is recorded every time；If the RSD of each active component is respectively less than 5%, illustrate efficient liquid phase
Chromatograph precision is good；
4) under chromatographic condition, the sample solution injection high performance liquid chromatograph of 10 μ L is detected, continuous sample introduction 6
It is secondary, the chromatographic peak peak area of each active component is recorded every time；If the RSD of each active component is respectively less than 5%, illustrate sample solution
In each active component repeatability it is good；
5) under chromatographic condition, the sample solution of 10 μ L is injected separately into high performance liquid chromatograph in 0-24h different time
It is detected, records the chromatographic peak peak area of each active component in different time points；If the RSD of each active component less than 5%,
Illustrate that each active component has good stability in sample solution；
6) sample solution for taking 6 parts of 2.5mL is separately added into the reference substance solution of 2.5mL into every part of sample solution, obtains mixed
Close liquid；It under chromatographic condition, takes 10 μ L solution to be injected separately into high performance liquid chromatograph from every portion of mixed liquor and is detected, calculate
The rate of recovery of each active component in every portion of mixed liquor；If the RSD of each active component less than 5%, illustrates each work in sample solution
Property component recyclability is good；
The chromatographic condition is as follows：Detector：Diode array detector；Chromatographic column：YMC-Pack ODS-A C18 color
It composes column (150mm × 4.6mm, 5 μm)；Mobile phase：Acetonitrile (A)：0.6% (volume fraction of acetic acid aqueous solution) acetic acid (B)；A：
8%-28%, 0-30min (mobile phase washing time)；B：92%-72%, 0-30min；Detection wavelength：330nm；Flow velocity：
1.0mL/min；Column temperature：25℃；
Wherein, A：8%-28%, 0-30min (mobile phase washing time)；B：92%-72%, 0-30min；Expression is being flowed
In dynamic phase washing time 0-30min, A phase and the variation range of B phase volume fraction are respectively A in chromatographic column：8%-28%, B：
92%-72%.
The preparation of mobile phase：Precision measures 6mL acetic acid, sets in 1000mL volumetric flask, using ultrapure water constant volume, mixes, mistake
0.22 μm of organic miillpore filter, ultrasound exhaust 15min.
Mobile phase of the invention needed 0.22 μm of organic miillpore filter before entering high performance liquid chromatography.
Proportionate relationship having the same between each active component in the reference substance solution and sample solution.
Further, the preparation of the sample solution includes the following steps:
1) honeysuckle of crushing is placed in stuffed conical flask, 50% methanol and weighed weight is then added, obtains concentration
For the mixed liquor of 0.02g/mL；The mixed liquor is ultrasonically treated 30-50min, releases the active component of honeysuckle effectively；
The weight for supplying loss after ultrasonic treatment with 50% methanol, makes before ultrasound and mixed liquor is equal in weight after ultrasound；
2) mixed liquor after ultrasound is shaken up, and filtering and removing slag；It takes filtrate to cross 0.22 μm of organic filter membrane, obtains honeysuckle
50% methanol solution.
Further, the preparation of the reference substance solution includes the following steps：Precision weighs 2.18mg neochlorogenic acid, 1.05mg
Cryptochlorogenic acid, 4.04mg rutin, 2.42mg galuteolin, 1.53mg 3,4-Dicaffeoylquinic acid, are put into 250mL brown volumetric flask, use
50% methanol solution constant volume dissolves, and mixes, obtains the first reference substance solution；Precision weighs the different green original of 6.16mg chlorogenic acid, 0.97mg
Sour C and 2.86mg 3,5-Dicaffeoylquinic acid, is put into 25 brown volumetric flasks, and first reference substance solution is added, and constant volume mixes, and mixes
It is even；The organic filter membrane for crossing 0.22 μm, obtains reference substance solution；
Wherein, the concentration to mixing according to each component in product solution is respectively：0.00872 μ g/ μ L of neochlorogenic acid, chlorogenic acid
0.246 μ g/ μ L, 0.0042 μ g/ μ L of Cryptochlorogenic acid, 0.01616 μ g/ μ L of rutin, 0.00968 μ g/ μ L of galuteolin, isochlorogenic acid
0.00612 μ g/ μ L of B, 0.1144 μ g/ μ L of 3,5-Dicaffeoylquinic acid, 0.0388 μ g/ μ L of 4,5-Dicaffeoylquinic acid.
Further, the condition ultrasonic treatment that the active component in honeysuckle is released completely to be met is as follows：Ultrasound
Power 600w, supersonic frequency 40KHZ, 40 DEG C of ultrasonic temperature.
Further, eight kinds of active components that this method can simultaneously in test sample solution；The active component is that fresh green is former
Acid, chlorogenic acid, Cryptochlorogenic acid, rutin, galuteolin, 3,4-Dicaffeoylquinic acid, 3,5-Dicaffeoylquinic acid and 4,5-Dicaffeoylquinic acid.
Further, the separating degree in the sample solution between each active component is greater than 1.5, and each active component is in respective model
Enclose interior R2It is all larger than 0.9990；In repeatability, stability, sample recovery rate and Precision Experiment, the RSD of each active component is equal
Less than 5%.
Further, in order to make the pillar of reference substance solution, sample solution and mobile phase not blocking highly effective liquid chromatograph；Cause
This reference substance solution, sample solution and mobile phase were required to 0.22 μm of organic filter membrane.
Beneficial effects of the present invention are as follows：
Method of the invention is combined using high performance liquid chromatography and uv detection method, has detected the extraction of 50% methanol of honeysuckle
Eight kinds of effective components and its content in liquid；By methodological study, show using method it is good, can be used as while measuring gold
Neochlorogenic acid in 50% methanol extract of honeysuckle flower, chlorogenic acid, Cryptochlorogenic acid, rutin, galuteolin, 3,4-Dicaffeoylquinic acid, 3,5-Dicaffeoylquinic acid,
The method of 4,5-Dicaffeoylquinic acid, it is accurate that the method is stablized, and sensitive efficient, the rate of recovery is high.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.
Fig. 1:(a) chromatogram of reference substance solution is shown；(b) chromatogram of 50% methanol solution of honeysuckle is shown.
Specific embodiment
In order to illustrate more clearly of the present invention, the present invention is done further below with reference to preferred embodiments and drawings
It is bright.It will be appreciated by those skilled in the art that specifically described content is illustrative and be not restrictive below, it should not be with this
It limits the scope of the invention.
Instrument of the present invention：High performance liquid chromatograph (e2695), water generation company of the U.S.；Ultrasonic cleaner
(KQ600E), Kunshan Ultrasonic Instruments Co., Ltd.；Vacuum pump, Tianjin Ao Tesaiensi Instrument Ltd.；Assay balance, plum
Teller-support benefit group；Pure water meter, Shanghai power new instrument Co., Ltd.
Test material of the invention：Honeysuckle is purchased from Beijing Tongrentang；Neochlorogenic acid (lot number：16031121), chlorogenic acid
(lot number：14031321), Cryptochlorogenic acid (lot number：16030331), rutin (lot number：14061322), galuteolin (lot number：
15073023), 3,4-Dicaffeoylquinic acid (lot number：15111921), 3,5-Dicaffeoylquinic acid (lot number：And 4,5-Dicaffeoylquinic acid (lot number 15111925)：
15081422) it is purchased from Shanghai Tongtian Biotechnology Co., Ltd.；Acetonitrile (chromatographically pure) and methanol (chromatographically pure) are purchased from Tianjin
Fine chemistry industry research institute is recovered in city.
Embodiment 1
The preparation of 50% methanol solution of honeysuckle
After honeysuckle smashes, Honeysuckle Flower 1.000g is weighed, is placed in 50mL stuffed conical flask, 50% methanol is added
50mL, weighed weight are ultrasonically treated (power 600W, frequency 40KHZ, 40 DEG C of temperature) 30min, let cool, weighed weight, with 50%
Methanol supplies bodies lost weight, shakes up, filtering, for use, the organic filter membrane for taking subsequent filtrate to cross 0.22 μm to get.Prepare 6 parts of Duplicate Samples
Product.
The preparation of reference substance solution
Precision weigh 2.18mg neochlorogenic acid, 1.05mg Cryptochlorogenic acid, 4.04mg rutin, 2.42mg galuteolin,
1.53mg 3,4-Dicaffeoylquinic acid is put into 250mL brown volumetric flask, with 50% methanol solution constant volume, is dissolved, is mixed, obtain the first control
Product solution；6.16mg chlorogenic acid, 0.97mg 4,5-Dicaffeoylquinic acid and 2.86mg 3,5-Dicaffeoylquinic acid are weighed respectively, are put into 25mL brown capacity
In bottle, first reference substance solution is added, constant volume mixes, and mixes；0.22 μm of organic filter membrane is crossed, for use.
Chromatographic condition
Detector：Diode array detector；
Chromatographic column：YMC-Pack ODS-A C18 chromatographic column (150mm × 4.6mm, 5 μm)；
Mobile phase：Acetonitrile (A)：0.6% acetic acid (B)；A：8%-28%, 0-30min；B：92%-72%, 0-30min；
Detection wavelength：330nm；
Flow velocity：1.0mL/min；
Column temperature：25℃.
Embodiment 2
A method of honeysuckle active component is measured using HPLC method, is included the following steps：
1) specificity is tested
Under chromatographic condition, 50% methanol solution of honeysuckle and reference substance solution of 10 μ L are injected into high-efficient liquid phase color respectively
Spectrometer is detected, the result is shown in Figure 1 (a) and (b)；As shown in Figure 1, the separating degree of the adjacent chromatographic peak of two kinds of solution is all larger than 1.5,
And at the corresponding retention time of each active component of reference substance, each active component of sample also has corresponding chromatographic peak to occur；It says
It is separable, reference between each active component in bright 50% methanol solution of honeysuckle《Pharmacopoeia of People's Republic of China》,
2010, annex 37.
The calculation formula of separating degree R：R=2 (tR2-tR1)/(W1+W2)；Wherein, tR2:The reservation at latter peak in adjacent two peak
Time；tR1:The retention time at previous peak in adjacent two peak；W1,W2:Distinguish the peak width at adjacent two peak thus.
2) linear relationship is investigated
Under chromatographic condition, 2.5,5,10,15,20,25 μ L of reference substance solution is drawn respectively and injects high performance liquid chromatograph
It is detected, with sample volume (μ g) for abscissa (x), is drawn using each active component chromatographic peak integrating peak areas value as ordinate (y)
Standard curve processed.Regression equation is shown in Table 1, the R of each active component of reference substance solution2It is all larger than 0.99, illustrates that 8 kinds of active components exist
Linear relationship is good in respective range.
The linear relationship of each active component of table 1
3) precision test
Under chromatographic condition, the reference substance solution injection high performance liquid chromatograph of 10 μ L is detected, continuous sample introduction 6
It is secondary, the chromatographic peak peak area of each active component is recorded every time, the results are shown in Table 2；Table 2 shows each active component RSD of reference substance solution
It is 0.39%~1.78%, illustrates that instrument precision is good.Relative standard deviation (RSD)=standard deviation (SD)/calculated result
Arithmetic mean of instantaneous value (X) × 100%.
2 precision of table investigates result
4) repetitive test
Under chromatographic condition, 50% methanol solution of the honeysuckle injection high performance liquid chromatograph of 10 μ L is detected, even
Continuous sample introduction 6 times, the chromatographic peak peak area of each active component is recorded every time；It the results are shown in Table 3；Table 3 shows that each active component RSD is
0.35%-1.59% then illustrates that each active component repeatability is good in 50% methanol solution of honeysuckle.
3 repeatability of table investigates result
5) stability test
Under chromatographic condition, respectively at 0,2,4,8,12 and for 24 hours draw 10mL 50% methanol solution of honeysuckle and inject
High performance liquid chromatograph is detected, and is recorded chromatogram, is calculated each active component chromatographic peak peak area, the results are shown in Table 4；8 in table 4
Kind effective component peak area RSD is 0.50%~2.43%, shows that 50% methanol solution of honeysuckle is stablized interior for 24 hours.
4 study on the stability result of table
6) sample recovery rate is tested
6 parts of 50% methanol solution of honeysuckle for having measured 8 kinds of active constituent contents is measured, every part of 2.5mL sets 5mL brown
In volumetric flask, it is separately added into reference substance solution 2.5mL, under chromatographic condition, separately sampled 10 μ l simultaneously injects high performance liquid chromatography
Instrument；The sample recovery rate for calculating each active component, the results are shown in Table 5.Notably reference substance each ingredient in sample-adding recovery test
Add the sum of people's amount and ingredient amount tested in test sample must be within the standard curve range of linearity；The amount of the reference substance of addition
It is appropriate, it is too small, cause biggish relative error, excessive then interference component is opposite to be reduced, and authenticity is poor.
Sample recovery rate %=(C-A)/B × 100%；Wherein, A is the original amount of each ingredient of 50% methanol solution of honeysuckle；B
For ingredient additional amount each in reference substance；C is each ingredient measured amount.With reference to《Pharmacopoeia of People's Republic of China》, 2010, annex 130.
5 sample recovery rate test result (n=6) of table
As shown in Table 5, the average recovery rate of each active component is 98.98~102.84% in 50% methanol solution of honeysuckle
(n=6), RSD 0.9%-4.11% illustrates that the rate of recovery of each active component in 50% methanol solution of honeysuckle is good.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention may be used also on the basis of the above description for those of ordinary skill in the art
To make other variations or changes in different ways, all embodiments can not be exhaustive here, it is all to belong to this hair
The obvious changes or variations that bright technical solution is extended out are still in the scope of protection of the present invention.
Claims (6)
1. a kind of method using HPLC method measurement honeysuckle active component, which is characterized in that include the following steps：
1) under chromatographic condition, sample solution and reference substance solution is injected separately into high performance liquid chromatograph and detected, is compared
The separating degree of the adjacent chromatographic peak of two kinds of solution；If the separating degree is greater than 1.5, illustrate that each active group in sample solution divides it
Between be separable；
2) under chromatographic condition, the reference substance solution of 2.5-25 μ L different volumes is injected separately into high performance liquid chromatograph and is examined
It surveys, with sample volume (μ g) for abscissa (x), draws standard by ordinate (y) of each active component chromatographic peak integrating peak areas value
Curve, if the R of each active component2It is all larger than 0.9, then illustrates that each active component in sample solution is linearly closed in respective range
System is good；
3) under chromatographic condition, the reference substance solution injection high performance liquid chromatograph of 10 μ L is detected, continuous sample introduction 6 times,
The chromatographic peak peak area of each active component is recorded every time；If the RSD of each active component is respectively less than 5%, illustrate high-efficient liquid phase color
Spectrometer precision is good；
4) under chromatographic condition, the sample solution injection high performance liquid chromatograph of 10 μ L is detected, continuous sample introduction 6 times, often
The secondary chromatographic peak peak area for recording each active component；If the RSD of each active component is respectively less than 5%, illustrate each in sample solution
Active component repeatability is good；
5) under chromatographic condition, the sample solution of 10 μ L is injected separately into high performance liquid chromatograph in 0-24h different time and is carried out
Detection records the chromatographic peak peak area of each active component in different time points；If the RSD of each active component illustrates less than 5%
Each active component has good stability in sample solution；
6) sample solution for taking 6 parts of 2.5mL is separately added into the reference substance solution of 2.5mL into every part of sample solution, must mix
Liquid；It under chromatographic condition, takes 10 μ L solution to be injected separately into high performance liquid chromatograph from every portion of mixed liquor and is detected, calculate every
The rate of recovery of each active component in part mixed liquor；If the RSD of each active component less than 5%, illustrates each activity in sample solution
Component recyclability is good；
The chromatographic condition is as follows：Detector：Diode array detector；Chromatographic column：YMC-Pack ODS-A C18 chromatographic column
(150mm×4.6mm,5μm)；Mobile phase：Acetonitrile (A)：0.6% acetic acid (B)；A：8%-28%, 0-30min；B：92%-
72%, 0-30min；Detection wavelength：330nm；Flow velocity：1.0mL/min；Column temperature：25℃；
Proportionate relationship having the same between each active component in the reference substance solution and sample solution.
2. the method according to claim 1, wherein the preparation of the sample solution includes the following steps:
1) honeysuckle of crushing is placed in stuffed conical flask, 50% methanol and weighed weight is then added, obtaining concentration is
The mixed liquor of 0.02g/mL；The mixed liquor is ultrasonically treated 30-50min, releases the active component of honeysuckle effectively；It is super
The weight for supplying loss after sonication with 50% methanol, makes before ultrasound and mixed liquor is equal in weight after ultrasound；
2) mixed liquor after ultrasound is shaken up, and filtering and removing slag；It takes filtrate to cross 0.22 μm of organic filter membrane, obtains 50% first of honeysuckle
Alcoholic solution.
3. the method according to claim 1, wherein the preparation of the reference substance solution includes the following steps：Essence
It is close to weigh 2.18mg neochlorogenic acid, 1.05mg Cryptochlorogenic acid, 4.04mg rutin, 2.42mg galuteolin, 1.53mg isochlorogenic acid
B is put into 250mL brown volumetric flask, with 50% methanol solution constant volume, is dissolved, is mixed, obtain the first reference substance solution；Precision claims
6.16mg chlorogenic acid, 0.97mg 4,5-Dicaffeoylquinic acid and 2.86mg 3,5-Dicaffeoylquinic acid are taken, is put into 25mL brown volumetric flask, described in addition
First reference substance solution, constant volume mix, and mix；The organic filter membrane for crossing 0.22 μm, obtains reference substance solution；
Wherein, the concentration of each component is respectively in reference substance solution：0.00872 μ g/ μ L of neochlorogenic acid, 0.246 μ g/ μ of chlorogenic acid
L, 0.0042 μ g/ μ L of Cryptochlorogenic acid, 0.01616 μ g/ μ L of rutin, 0.00968 μ g/ μ L of galuteolin, 3,4-Dicaffeoylquinic acid 0.00612
μ g/ μ L, 0.1144 μ g/ μ L of 3,5-Dicaffeoylquinic acid, 0.0388 μ g/ μ L of 4,5-Dicaffeoylquinic acid.
4. according to the method described in claim 2, it is characterized in that, at the ultrasound that the active component in honeysuckle is released completely
It is as follows to manage the condition to be met：Ultrasonic power 600w, supersonic frequency 40KHZ, 40 DEG C of ultrasonic temperature.
5. the method according to claim 1, wherein this method can simultaneously in test sample solution eight kinds of activity
Component；The active component is neochlorogenic acid, chlorogenic acid, Cryptochlorogenic acid, rutin, galuteolin, 3,4-Dicaffeoylquinic acid, 3,5-Dicaffeoylquinic acid
And 4,5-Dicaffeoylquinic acid.
6. -6 any method according to claim 1, which is characterized in that in the sample solution between each active component
Separating degree is greater than 1.5, each active component R in respective range2It is all larger than 0.9990；In repeatability, stability, sample recovery rate
In Precision Experiment, the RSD of each active component is respectively less than 5%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109633013A (en) * | 2018-12-29 | 2019-04-16 | 重庆医药高等专科学校 | A kind of honeysuckle multi objective quantitative analysis method |
| CN111474272A (en) * | 2020-03-31 | 2020-07-31 | 中国检验检疫科学研究院 | Method and kit for detecting chlorogenic acid compound |
| CN112903841A (en) * | 2021-01-18 | 2021-06-04 | 中国检验检疫科学研究院 | Method for detecting isomers of chlorogenic acids |
| CN113607847A (en) * | 2021-08-03 | 2021-11-05 | 山东中医药大学 | Method for determining contents of various phenolic acid components in honeysuckle |
| CN113607848A (en) * | 2021-08-03 | 2021-11-05 | 山东中医药大学 | Method for simultaneously measuring multiple phenolic acids in honeysuckle |
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| CN113607848A (en) * | 2021-08-03 | 2021-11-05 | 山东中医药大学 | Method for simultaneously measuring multiple phenolic acids in honeysuckle |
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Application publication date: 20181116 |