CN102621084B - Method for measuring soil ammonium nitrogen - Google Patents
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Abstract
The invention discloses a method for measuring soil ammonium nitrogen. The method is characterized in that soil ammonium nitrogen extracting solution is firstly extracted by the aid of NaCl water solution with the mass percent concentration of 20% in an oscillating manner, supernate is centrifugally separated, zinc sulfate water solution with the mass percent concentration of 10% and sodium hydroxide water solution with the mass percent concentration of 25% are sequentially added, shaken up, settled by means of standing and filtered, filtrate is extracted after constant volume is set, NaCl water solution with the mass percent concentration of 20% is added, Nessler reagents are added and shaken up to realize color development reaction with ammonium ions, a cuvette with the optical path of 10mm is used for measuring the absorbance of a position with the wavelength of 420nm by using a blank as a reference, and the concentration of the ammonium nitrogen in the extracting solution is calculated by substituting the absorbance in a calibration curve regression equation. Addition of masking agents is omitted, chemical reagents are saved, procedures are simplified, and the method is simple, wide in linear range, stable in color development, easy in operation, fine in repeatability, fewer in error, fast in measuring speed and suitable for popularization and application.
Description
Technical field
The invention belongs to ammonium nitrogen detection method technical field, being specifically related to the nessler reagent is the analytical test method that developer is measured soil ammonium.
Background technology
" soil physical analysis " (Shanghai science tech publishing house that Nanjing Soil Inst., Chinese Academy of Sciences writes, 1978,81-84 page or leaf) points out that nitrogen is one of three essential big nutrients of plant in, growth and development of plant is had significant impact, and soil is the main source of crop nitrogen nutrition.Ammonium nitrogen in the soil is directly absorbed by crop easily, belongs to quick-acting nitrogen." agriculture nitrogen management and environmental quality " (Ma Lishan, Jiangsu science tech publishing house, 1992) point out in, along with nitrogenous fertilizer a large amount of uses worldwide, because that does not consider soil uses nitrogenous fertilizer blindly in a large number for the nitrogen ability, make nitrogen utilization efficiency low on the one hand, cause the massive losses of nitrogen; Cause serious environmental to be polluted because of the volatilization loss of ammonium nitrogen and some oxides of nitrogen, nitrate leaching on the other hand, can and healthyly cause very big harm to people's life.So; the soil ammonium-state Determination on content is worth for the fertility of estimating soil, and guidance applies fertilizers scientifically, and the environmental benefit of assessment nitrogen supply; many aspects such as the protection ecologic equilibrium are all significant, and the needs that quick, easy, accurate, sensitive assay method has become a reality.
" soil agrochemistry the conventional method of analysis " (Beijing of writing according to Soil Science Society of China agriculture chemistry Professional Committee, Science Press, 1983, the 84-88 page or leaf) the middle introduction, the assay method of soil ammonium is measured two classes after mainly being divided into direct distillation and lixiviate.Direct distillation easily makes the result higher, so the KCl aqueous solution that general at present employing concentration is 2N leaches NH in the soil
4 +-N (NH
4 +Be ammonium, with NH
4 +-N represents ammonium nitrogen), NH in the leachate
4 +-N can select for use methods such as distillation, colorimetric or ammonia electrode to measure.Such as " agricultural science and technology and information " (2008, the 15:40-41 page or leaf) having introduced a kind of employing KCl solution in " determination and analysis of nitrogen content in the soil " literary composition extracts, filters, with MgO ammonia is distilled then, absorb with boric acid again, then with the method for sour standard solution titration, but the determination step of this method is more loaded down with trivial details." environmental science and technology " (2006,29 (1): the 43-45 page or leaf) method of being taked in " research of nessler reagent colorimetric method for determining soil ammonium " literary composition is: take by weighing through air-dry, grind, the pedotheque 10g of division gained is in triangular flask, the adding mass percent concentration is 20% NaCl solution 50mL, behind the vibration 30min, filter with qualitative filter paper, draw 5mL filtrate in the 25mL color comparison tube, thin up to the 20mL after, adding 1mL potassium sodium tartrate solution and 1.5mL mass percent concentration are 20% NaCl solution, fully shake, add the 0.5mL nessler reagent after leaving standstill 15min, the limit edged shakes, be settled to scale then, measure absorbance in the 390nm place with spectrophotometer after a period of time.But general because the soil constituent more complicated has a lot of impurity and is dissolved in the NaCl solution, after adding sodium potassium tartrate tetrahydrate, many times can influence the colorimetric result because of producing flocculent deposit easily; Itself just there is error in the qualitative filter paper filtration that the method adopted in addition, because paper technology adopts the papermaking of ammonium method more, having a spot of ammonium salt unavoidably remains in the filter paper, water flushing in any case, and filtrate at the beginning of abandoning or adopting, also can not remove the ammonia nitrogen that contains in the filter paper fully, thereby have influence on the accuracy of measurement result.
Summary of the invention
The assay method that the purpose of this invention is to provide a kind of soil ammonium makes testing result favorable reproducibility, good stability, error little to overcome the above-mentioned defective of prior art, to reach, and easy operating.
The present invention measures the method for soil ammonium, it is characterized in that operation steps is as follows:
Adding the 5-8ml mass percent concentration by every gram soil sample to be measured is 20 ± 0.5% NaCl aqueous solution, on the earthquake machine with the rate oscillation 40-60min of 150 ± 20r/min, resulting extract is placed centrifuge tube, with 3000-5000 commentaries on classics/min centrifuging 5-10min; The supernatant that centrifuging is obtained, adding 0.1 ± 0.005ml mass percent concentration according to every 10-30ml soil supernatant is that 10 ± 0.5% zinc sulfate solution and 0.1-0.3ml mass percent concentration are 25 ± 0.5% sodium hydrate aqueous solution, after shaking up, leave standstill to producing flocculation sediment, be that glass sand core funnel or the filter membrane of 0.1-0.45 μ m filters with the aperture then, gained filtrate adds water and is settled to 25ml; Get that 2-6ml is above-mentioned to add water constant volume filtrate in color comparison tube, adding mass percent concentration by 1: 1 volume ratio is 20% NaCl aqueous solution, add the 0.5ml nessler reagent then, shake up, add water and be settled to 25ml, leave standstill 10min after, with the cuvette of light path 10mm, with the blank is reference, measures the absorbance at wavelength 420nm place; With this absorbance substitution calibration curve regression equation, calculate ammonium nitrogen concentration in the extract;
Described nessler reagent is prepared as follows: get 16g NaOH, be dissolved in the 30-40ml water and be mixed with sodium hydrate aqueous solution, be cooled to room temperature; Other gets 7g potassium iodide and 10g mercuric iodixde (HgI
2) be dissolved in the 30-50ml water, under agitation inject the sodium hydrate aqueous solution of above-mentioned preparation slowly, be diluted with water to 100ml, airtight preservation is standby;
Described calibration curve regression equation obtains by following way:
Get 0.3819g through 100 ℃ of dry water-soluble 100ml of being diluted to of the pure ammonium chloride of top grade that cross, promptly obtain every milliliter of standard stock solution that contains 1.00mg ammonium nitrogen;
Pipette the above-mentioned standard stock solution of 0.1ml, be diluted with water to 100ml, promptly obtain every milliliter of ammonium standard that contains 0.001mg ammonium nitrogen and use liquid;
Get 0ml (dummy), 0.1ml, 0.25ml, 0.5ml, 1m, 2ml, 4ml and 5ml ammonium standard and use liquid, place color comparison tube respectively, in each color comparison tube, add the 0.5ml nessler reagent respectively, shake up, add water then and be settled to 25ml, leave standstill 10 minutes after, pour into respectively in the cuvette that light path is 10mm, with wherein the 1st dummy is reference, measures in each cuvette sample respectively in the absorbance at wavelength 420nm place; With the absorbance is that ordinate, ammonium nitrogen content are that horizontal ordinate is drawn the typical curve that is formed by connecting by each sample point, is guaranteeing that linearly dependent coefficient R is R
2Under 〉=0.9990 condition, this typical curve is carried out linear regression, obtain calibration curve regression equation: y=Ax+B; Wherein y is an absorbance; X is an ammonium nitrogen content, and mg gets in unit; A and B are respectively the slope and the intercept of equation.
Because the improvement innovation that the present invention is centrifugal after measuring in original nessler reagent method on the basis of soil ammonium soil extract being carried out flocculation sediment, filter, thereby simplified determination step, color stability and easy operating, favorable reproducibility, error are little.
Compare with existing assay method,, help the stripping of soil ammonium-state owing to taked the prolongation concussion time in the inventive method; Owing to adopt centrifuging, shortened filtration time, reduced the free volatilization loss of ammonium nitrogen in the solution; Taking filtrate is carried out flocculation sediment is in order to eliminate water sample color or muddy and some other interfering materials, in order to avoid influence the mensuration of ammonium nitrogen; Adopt sand core funnel or filter membrane to filter and eliminated existing method usually because of adopting filter paper itself to contain the error that ammonia brings.Because the inventive method has adopted method centrifugal behind the flocculation sediment, that filter to soil extract, therefore need not to add screening agent, has saved medicine, has simplified program.Assay method of the present invention is simple to operate, speed is fast, is suitable for basic unit and agricultural sector applies.
Description of drawings
Fig. 1 is the calibration curve linearity of regression equation of the soil ammonium that adopts assay method of the present invention and record in embodiment 1-3.
Embodiment
Embodiment 1:
The first step, the extraction of soil sample ammonium nitrogen liquid to be measured: take by weighing fresh soil sample 5g and place the 100ml triangular flask, adding 25ml mass percent concentration is 20 ± 0.5% NaCl aqueous solution, 40-60min is extracted in concussion speed concussion with 150 ± 20r/min on the earthquake machine, obtains soil sample ammonium nitrogen extract to be measured;
Second step, centrifuging: soil sample ammonium nitrogen extract to be measured is transferred in the 50ml tygon centrifuge tube, at 3000-5000 commentaries on classics/min centrifuging 5-10min;
The 3rd step, handle extract: the supernatant that above-mentioned centrifuging obtained is transferred in the test tube, adding successively and adding 0.1 ± 0.005ml mass percent concentration according to every 10-30ml soil supernatant is that 10 ± 0.5% zinc sulfate solution and 0.1-0.3ml mass percent concentration are 25 ± 0.5% sodium hydrate aqueous solution, carry out flocculation sediment, after shaking up, leave standstill and made precipitation in 30 minutes, be that glass sand core funnel or the filter membrane of 0.1-0.45 μ m filters with the aperture then, gained filtrate adds water and is settled to 25ml;
The 4th step, colorimetric estimation:
Earlier prepare nessler reagent as follows: take by weighing 16g NaOH, be dissolved in the 30-40ml water and be mixed with sodium hydrate aqueous solution, fully be cooled to room temperature; Other takes by weighing 7g potassium iodide and 10g mercuric iodixde (HgI
2) be dissolved in and make potassium iodide and mercuric iodixde mixed solution in the 30-50ml water, then this potassium iodide and mercuric iodixde mixed solution are under agitation injected the sodium hydrate aqueous solution of above-mentioned preparation slowly, be diluted with water to 100ml again, store in the polyethylene bottle or in other alkaline-resisting container, close plug is preserved.
Get that 2-6ml is above-mentioned to add water constant volume filtrate in color comparison tube, adding mass percent concentration by 1: 1 volume ratio is 20% NaCl aqueous solution, add the 0.5ml nessler reagent then, shake up, add water and be settled to 25ml and (take specifically to get 5ml filtrate in the present embodiment in the color comparison tube of 50ml, add the 5ml mass percent concentration and be 20% NaCl aqueous solution, the nessler reagent that adds the above-mentioned preparation of 0.5ml then, shake up, make it to carry out chromogenic reaction, add water then and be settled to 25ml) with ammonium ion; After placing 10min,,, be reference, measure absorbance y with the blank with the cuvette of light path 10mm at wavelength 420nm place.
Measure below and make acquisition calibration curve regression equation, operate according to following way:
Preparation ammonium nitrogen standard stock solution: it is soluble in water through 100 ℃ of dry pure ammonium chlorides of top grade of crossing accurately to take by weighing 0.3819g, moves in the 100ml volumetric flask, is diluted to scale, and this solution is every milliliter and contains 1.00mg ammonium nitrogen.
Preparation ammonium standard is used liquid: pipette the above-mentioned ammonium nitrogen of 0.1ml standard stock solution in the 100ml volumetric flask, be diluted with water to scale, this solution is every milliliter and contains 0.001mg ammonium nitrogen.
Draw 0ml (dummy), 0.1ml, 0.25ml, 0.5ml, 1m, 2ml, 4ml and 5ml ammonium standard and use liquid, place the 50ml color comparison tube respectively, add the 0.5ml nessler reagent more respectively, shake up, add water then and be settled to 25ml, after 10 minutes, respectively the solution in the above-mentioned color comparison tube is poured into the cuvette of 10mm, with wherein the 1st dummy is reference, measures the absorbance at wavelength 420nm place; Be that ordinate, ammonium nitrogen content are the horizontal ordinate described point then with the absorbance, the line linearity of going forward side by side returns, and obtains calibration curve regression equation: y=Ax+B, requires linearly dependent coefficient R
2〉=0.9990; Wherein y is an absorbance; X is an ammonium nitrogen content, and mg gets in unit; A and B are respectively the slope and the intercept of equation; R is a linearly dependent coefficient.
Described linearly dependent coefficient R requires the linear dependence of y and x exactly to the typical curve of being done, linearly dependent coefficient R value is big more, illustrate that line that the data point measured retouches out is more near straight line on coordinate axis, otherwise the data point of mensuration does not form straight line, just can not be as calibrating curve equation.The calibration curve linearity of regression equation of the soil ammonium that can record in an embodiment with reference to figure 1, Fig. 1 cathetus returns according to those measured values (pore) and obtains, if pore is far away more from straight line, explain deviations is big more, the value of R is more little, just can not be when the value of linearly dependent coefficient R as typical curve less than 0.999, must redeterminate; Can utilize software to calculate linear regression on computers, also can adopt hand computation.The typical curve that mensuration obtains can be used among the several embodiment that measure on same the machine.
By said determination, satisfy R in the present embodiment in linear coefficient R
2Under=0.9998 the condition, the calibrating curve equation that obtains is: y=5.2874x+0.0032;
Calculate among the absorbance y substitution calibration curve regression equation y=5.2874x+0.0032 of the pedotheque that institute's present embodiment is recorded then, calculate ammonium nitrogen content x mg in the 5ml extract, draw that ammonium nitrogen content is 0.0338mg in the 5g soil sample ammonium nitrogen extract, i.e. soil ammonium concentration 0.0068mg/g.
Soil ammonium-state content calculates according to w=ax/m, and in the formula: w is a soil ammonium-state concentration, mg/g; X is an ammonium nitrogen content in the 5ml extract, mg; A=V/5, a are the extension rate that mensuration is got the 5ml sample extracting solution, and V is that extract is overall; M is a soil quality, g.(annotate: the sample volume of being got during mensuration can be 5ml, also can get the arbitrary value less than 15ml.)
Wherein involved various solution all adopt the corresponding national standards analytical approach to prepare, and compound method is:
Mass percent concentration is the preparation of 20% NaCl aqueous solution: it is water-soluble to take by weighing 20gNaCl, is diluted to 100ml.
Mass percent concentration is the preparation of 10% zinc sulfate solution: it is water-soluble to take by weighing 10g zinc sulfate, is diluted to 100ml.
Mass percent concentration is the preparation of 25% sodium hydrate aqueous solution: it is water-soluble to take by weighing 25g NaOH, is diluted to 100ml, stores in by (or in other alkaline-resisting container) in the polyethylene bottle.
Embodiment 2:
The first step, extract soil sample ammonium nitrogen liquid to be measured: take by weighing fresh soil sample 1g and put into the 100ml triangular flask, adding 25ml mass percent concentration is 20 ± 0.5% NaCl aqueous solution, and 50min is extracted in the concussion speed concussion with 160r/min on the earthquake machine, obtains soil sample ammonium nitrogen liquid to be measured;
Second step, centrifuging: shift all extracts in 50ml tygon centrifuge tube, under the condition of 4000 commentaries on classics/min, carry out centrifuging 8min;
The 3rd step, handle extract: the supernatant that centrifuging is obtained is transferred in the test tube, add 0.3 ± 0.005ml mass percent concentration successively and be 10 ± 0.5% zinc sulfate solution and 0.2ml mass percent concentration and be 25 ± 0.5% sodium hydrate aqueous solution and carry out flocculation sediment, after shaking up, be placed to and produce precipitation, with the aperture is that the filter membrane of 0.45 μ m filters, and gained filtrate is added water be settled to 25ml;
The 4th step, colorimetric estimation: get 5ml filtrate in the color comparison tube of 50ml, adding 5ml mass percent concentration is 20% NaCl aqueous solution, adds the 0.5ml nessler reagent then, shake up, make it to carry out chromogenic reaction, add water then and be settled to 25ml, behind the placement 10min with ammonium ion, at wavelength 420nm place, with the cuvette of light path 10mm, be reference with the blank, measure absorbance.Then gained sample absorbance is brought among the calibration curve regression equation y=5.2874x+0.0032, calculate in the present embodiment that ammonium nitrogen content is 0.0065mg in the 1g soil sample ammonium nitrogen extract, be i.e. soil ammonium concentration 0.0065mg/g.
Embodiment 3:
The first step, extract soil sample ammonium nitrogen liquid to be measured: take by weighing fresh soil sample 3g and put into the 100ml triangular flask, adding 25ml mass percent concentration is 20 ± 0.5% NaCl aqueous solution, and 60min is extracted in the concussion speed concussion with 170r/min on the earthquake machine, obtains soil sample ammonium nitrogen liquid to be measured;
In second step, centrifuging is shifted all extracts in 50ml tygon centrifuge tube, carries out centrifuging 8min under the condition of 5000 commentaries on classics/min;
The 3rd step, handle extract: supernatant is transferred in the test tube, add 0.5 ± 0.005ml mass percent concentration successively and be 10 ± 0.5% zinc sulfate solution and 0.3ml mass percent concentration and be 25 ± 0.5% sodium hydrate aqueous solution and carry out flocculation sediment, after shaking up, be placed to and produce precipitation, with the aperture is the 5-3 type glass sand core funnel filtration of 0.45 μ m, gained filtrate is added water be settled to 25ml; The 4th step, colorimetric estimation is got 5ml filtrate in the color comparison tube of 50ml, and adding 5ml mass percent concentration is 20% NaCl aqueous solution, adds the 0.5ml nessler reagent then, shake up, make it to carry out chromogenic reaction, add water then and be settled to 25ml, behind the placement 10min with ammonium ion, at wavelength 420nm place, with the cuvette of light path 10mm, be reference with the blank, measure absorbance.Bring gained sample absorbance into the calibration curve regression equation then, calculate that ammonium nitrogen content is 0.0192mg in the 3g soil extract, be i.e. soil ammonium concentration 0.0064mg/g.
The resulting calibration curve regression equation of the above embodiment of the present invention experiment test is: y=5.2874x+0.0032, institute's line taking coefficient R
2=0.9998, in the formula: y is a light absorption value; X is an ammonium nitrogen content, and unit is mg; R is a linearly dependent coefficient.
To taking the different concussion time to carry out centrifuging in the foregoing description respectively, record the relation that the earthquake time carries out the stripping quantity result of centrifuging and soil ammonium-state when being respectively 8min, 6min and 10min, gained the results are shown in the following table 1, to compare analysis:
The table 1 concussion time is to the influence of the stripping of ammonium nitrogen in the same soil sample
| The concussion time, min | 10 | 20 | 30 | 40 | 50 | 60 | 70 | 80 |
| Ammonium nitrogen content, mg/g soil | 0.0332 | 0.0338 | 0.0349 | 0.0367 | 0.0373 | 0.0376 | 0.0376 | 0.0376 |
As seen from Table 1, the longer concussion time of taking helps the stripping of soil ammonium-state in the foregoing description.
Each mensuration is got single sample and is carried out parallel analysis twice, simultaneously do blank test with no aqueous ammonia to replace water sample, and represent that with result's mean value test findings shows that this method measurement result has good Repeatability and Reproducibility, its error satisfies the desired error level of conventional method of analysis.Table 2 has provided the error contrast that different soil samples are measured ammonium nitrogen:
The different soil samples of table 2 are measured the error contrast of ammonium nitrogen
Analysis by data in the his-and-hers watches 2 relatively can draw assay method measurement of the present invention accurately, and error is little, the conclusion of favorable reproducibility.
Because the improvement innovation that the present invention is centrifugal after measuring in original nessler reagent method on the basis of soil ammonium soil extract being carried out flocculation sediment, filter, thereby simplified determination step, color stability and easy operating, favorable reproducibility, error are little.
Compare with existing assay method, because the prolongation concussion time of being taked in the inventive method helps the stripping of soil ammonium-state; Owing to adopt centrifuging, shortened filtration time, reduced the free volatilization loss of ammonium nitrogen in the solution; Take in the inventive method filtrate is carried out flocculation sediment, to eliminate water sample color or muddy and some other interfering materials, in order to avoid influence the mensuration of ammonium nitrogen; Owing to adopt sand core funnel or filter membrane to filter, eliminated existing method usually because of adopting filter paper itself to contain the error that ammonia brings; Because the inventive method has adopted method centrifugal behind the flocculation sediment, that filter to soil extract, therefore need not to add screening agent, has saved medicine, has simplified program.Assay method of the present invention is simple to operate, speed is fast, is suitable for basic unit and agricultural sector applies.
Claims (1)
1. method of measuring soil ammonium is characterized in that operation steps is as follows:
Adding the 5-8ml mass percent concentration by every gram soil sample to be measured is 20 ± 0.5% NaCl aqueous solution, on the earthquake machine with the rate oscillation 40-60min of 150 ± 20r/min, resulting extract is placed centrifuge tube, with 3000-5000 commentaries on classics/min centrifuging 5-10min; The supernatant that centrifuging is obtained, adding 0.1 ± 0.005ml mass percent concentration according to every 10-30ml soil supernatant is that 10 ± 0.5% zinc sulfate solution and 0.1-0.3ml mass percent concentration are 25 ± 0.5% sodium hydrate aqueous solution, after shaking up, leave standstill to producing flocculation sediment, be that glass sand core funnel or the filter membrane of 0.1-0.45 μ m filters with the aperture then, gained filtrate adds water and is settled to 25ml; Get that 2-6ml is above-mentioned to add water constant volume filtrate in color comparison tube, adding mass percent concentration by 1: 1 volume ratio is 20% NaCl aqueous solution, add the 0.5ml nessler reagent then, shake up, add water and be settled to 25ml, leave standstill 10min after, with the cuvette of light path 10mm, with the blank is reference, measures the absorbance at wavelength 420nm place; With this absorbance substitution calibration curve regression equation, calculate ammonium nitrogen concentration in the extract;
Described nessler reagent is prepared as follows: get 16g NaOH, be dissolved in the 30-40ml water, be cooled to room temperature; Other gets the 7g potassium iodide and the 10g mercuric iodixde is dissolved in the 30-50ml water, under agitation injects the sodium hydrate aqueous solution of above-mentioned preparation slowly, is diluted with water to 100ml, and airtight preservation is standby;
Described calibration curve regression equation obtains by following way:
Get 0.3819g through 100 ℃ of dry water-soluble 100ml of being diluted to of the pure ammonium chloride of top grade that cross, promptly obtain every milliliter of standard stock solution that contains 1.00mg ammonium nitrogen;
Pipette the above-mentioned standard stock solution of 0.1ml, be diluted with water to 100ml, promptly obtain every milliliter of ammonium standard that contains 0.001mg ammonium nitrogen and use liquid;
Get 0ml, 0.1ml, 0.25ml, 0.5ml, 1m, 2ml, 4ml and 5ml ammonium standard and use liquid, place color comparison tube respectively, in each color comparison tube, add the 0.5ml nessler reagent respectively, shake up, add water then and be settled to 25ml, leave standstill 10 minutes after, pour into respectively in the cuvette that light path is 10mm, with wherein the 1st dummy is reference, measures in each cuvette sample respectively in the absorbance at wavelength 420nm place; With the absorbance is that ordinate, ammonium nitrogen content are that horizontal ordinate is drawn the typical curve that is formed by connecting by each sample point, is guaranteeing that linearly dependent coefficient R is R
2Under 〉=0.9990 condition, this typical curve is carried out linear regression, obtain calibration curve regression equation: y=Ax+B; Wherein y is an absorbance; X is an ammonium nitrogen content, and mg gets in unit; A and B are respectively the slope and the intercept of equation.
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| CN103760119A (en) * | 2014-01-14 | 2014-04-30 | 沈阳建筑大学 | Method for determining inhibition effect of plant sample on soil nitrification |
| CN105911055A (en) * | 2016-04-29 | 2016-08-31 | 南京信息工程大学 | Method for quickly detecting available nitrogen in soil |
| CN112014339A (en) * | 2020-08-28 | 2020-12-01 | 中检集团南方测试股份有限公司 | Determination method for quickly extracting ammonia nitrogen in soil |
| CN112082965A (en) * | 2020-09-18 | 2020-12-15 | 武汉工程大学 | Method for determining occurrence state of residual ammonium salt in weathered crust elution-deposited rare earth ore |
| CN112378869B (en) * | 2020-10-21 | 2022-12-27 | 南京曙光精细化工有限公司 | Method for detecting residual quantity of catalyst in polysulfide silane coupling agent |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008618A (en) * | 2007-01-13 | 2007-08-01 | 中国科学院等离子体物理研究所 | Method for real time in-situ tracking migration process of ammonium nitrogen fertilizer |
| CN101319999A (en) * | 2008-04-30 | 2008-12-10 | 河南农大迅捷测试技术有限公司 | Novel nessler's reagent and method for rapidly measuring soil ammonium nitrogen |
| CN101545866A (en) * | 2009-05-06 | 2009-09-30 | 北京市农林科学院 | Method for measuring content of inorganic nitrogen in soil |
-
2012
- 2012-03-14 CN CN 201210067740 patent/CN102621084B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008618A (en) * | 2007-01-13 | 2007-08-01 | 中国科学院等离子体物理研究所 | Method for real time in-situ tracking migration process of ammonium nitrogen fertilizer |
| CN101319999A (en) * | 2008-04-30 | 2008-12-10 | 河南农大迅捷测试技术有限公司 | Novel nessler's reagent and method for rapidly measuring soil ammonium nitrogen |
| CN101545866A (en) * | 2009-05-06 | 2009-09-30 | 北京市农林科学院 | Method for measuring content of inorganic nitrogen in soil |
Non-Patent Citations (6)
| Title |
|---|
| 叶丽娟 等.氨态氮测定方法的研究.《衡阳师范学院学报(自然科学)》.2002,第23卷(第3期),全文. |
| 弓晓峰 等.纳氏试剂比色法测定土壤铵态氮的研究.《环境科学与技术》.2006,第29卷(第1期),全文. |
| 氨态氮测定方法的研究;叶丽娟 等;《衡阳师范学院学报(自然科学)》;20020630;第23卷(第3期);全文 * |
| 纳氏试剂光度法测定水中氨态氮方法的探讨;董亚萍;《宁夏电力》;20041231;全文 * |
| 纳氏试剂比色法测定土壤铵态氮的研究;弓晓峰 等;《环境科学与技术》;20060131;第29卷(第1期);全文 * |
| 董亚萍.纳氏试剂光度法测定水中氨态氮方法的探讨.《宁夏电力》.2004,全文. |
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