CN102613178B - Cydia strobilella sex pheromone and preparation method thereof - Google Patents
Cydia strobilella sex pheromone and preparation method thereof Download PDFInfo
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- CN102613178B CN102613178B CN 201210059173 CN201210059173A CN102613178B CN 102613178 B CN102613178 B CN 102613178B CN 201210059173 CN201210059173 CN 201210059173 CN 201210059173 A CN201210059173 A CN 201210059173A CN 102613178 B CN102613178 B CN 102613178B
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- steinernema
- sex pheromone
- dragon spruce
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Abstract
The invention discloses a cydia strobilella sex pheromone, which comprises the following active ingredients: (E, E)-8, 10-dodecenylacetate and (E, Z)-8, 10-dodecenylacetate. The active ingredients of the pheromone are dissolved in organic solvent. The solution is produced into lure. The pheromone is simple in preparation technique and convenient to use. The sex pheromone can be used for monitoring, predicting and forecasting an emergence period of cydia strobilella so as to accurately know the emergence and regression situation of the cydia strobilella population in time and to provide scientific foundation to timely and effective control. The cydia strobilella sex pheromone also can be used for the generative prevention such as mass trapping and killing and interference in mating of the cydia strobilella.
Description
Technical field the present invention relates to a kind of insect sex pheromone, especially relates to dragon spruce cone steinernema sex pheromone.
(Lepidoptera Lepidoptera: Tortricidae Tortricidae: steinernema subfamily Olethreutinae) be distributed widely in the holarctic region spruce forest, be important cone insect to background technology dragon spruce cone steinernema Cydia strobilella (L.).Mainly be distributed in northeast, northwest and the Inner Mongol in China; Be distributed in Muscovite Siberia, the north, Europe, middle part and North America abroad.Main harm Europe Norway spruce Picea abies (L.) H.Karst, North America white spruce P.glauca (Moench) Voss and Picea mariana P.mariana (Miller), and sand ground dragon spruce P.mongolica (H.Q.Wu) W.D.Xu, the Picea koraiensis Nakai P.koraiensis Nakai of China, fish scale dragon spruce P.jezoensis Carr.var.microsperma and Dahurian larch Larix gmelini (Rupr.) Rupr. etc.The pollination phase of blooming of adult eclosion phase and host plant is synchronous, and female moth post-coitum is laid eggs between the female flower squama, directly parasitizes after the larva hatching and gets the food seed in the cone, thereby have a strong impact on seed production.It is reported, in recent years northeastward with forest zone, the Inner Mongol dragon spruce cone of causing harm in a large number, become one of primary pest of dragon spruce cone, can reach 95% at some regional dragon spruce cone rate of damage, average out to 30%, cone and seed production go from bad to worse, and not only having had a strong impact on collects seed grows seedlings and the self of sand ground spruce forest, and greatly hindered introducing a fine variety and afforesting of the serious area of desertifying, restricted the development that China's ecological forestry is built significantly.
Because the larval growth of dragon spruce cone steinernema is in cone, and the cone of being injured is difficult to by the external form identification, and simple use of insecticide is difficult to reach effective control.Therefore, for dragon spruce cone steinernema, sex pheromone is the means of potential useful Pest Management, because pheromones adult as target, usability pheromones trapper not only can be monitored its population quantity, and disturbs the harm that can control dragon spruce cone steinernema by trapping or mating.But, can effectively trap dragon spruce cone steinernema sex pheromone up to now and yet there are no report.
Summary of the invention the object of the present invention is to provide a kind of dragon spruce cone steinernema sex pheromone, and it can efficient, nuisanceless trapping dragon spruce cone steinernema, is used for population monitoring and the control of dragon spruce cone steinernema.
Another object of the present invention provides a kind of preparation method of dragon spruce cone steinernema sex pheromone.
Technical scheme of the present invention is as follows:
The active ingredient of dragon spruce cone steinernema sex pheromone is by instead, and is anti--8,10-dodecylene alcohol acetic ester and anti-, and suitable-8,10 dodecylene alcohol acetic esters are formed.
The weight portion of the active ingredient of above-mentioned dragon spruce cone steinernema sex pheromone is: anti-, anti--8, and 0～3.00 part of 10-dodecylene alcohol acetic ester, anti-, 0～3.00 part of suitable-8,10 dodecylene alcohol acetic ester.
The preferred version of the active ingredient of above-mentioned dragon spruce cone steinernema sex pheromone:
Instead, anti--8,0～0.3 part of 10-dodecylene alcohol acetic ester, anti-, 0.08～0.3 part of suitable-8,10 dodecylene alcohol acetic ester.
Described effective ingredient is dissolved in the organic solvent.
Described organic solvent is selected from a kind of in the following material: n-hexane, carrene, ether, acetone.
Described dragon spruce cone steinernema sex pheromone solution splashed into to make in the release vehicle lure core.
The application of described dragon spruce cone steinernema sex pheromone in monitoring, trapping and interference mating control dragon spruce cone steinernema.
Advantage and effect that the present invention has are:
1, the present invention is clear and definite can effectively lure the prescription of the sex pheromone of dragon spruce cone steinernema, and male moth is had very strong attracting ability, and preparation technology is simple, easy to use.
2, the present invention can be used for the population monitoring of dragon spruce cone steinernema, understands generation and the growth and decline situation of woodland dragon spruce cone steinernema population in time, exactly, provides scientific basis for prevent and treat effectively in good time.
3, the present invention can be used for the production control of dragon spruce cone steinernema adult.In the adult emergence period, in the heavier spruce forest of harm, generally discharge the sex pheromone of high dose of the present invention, can disturb the dragon spruce cone steinernema hero of seeking a spouse after the emergence, the normal mating of female moth effectively, make male moth searching less than female worm and lose the mating machine meeting, female moth can not raise up seed effectively, reduces harm.
4, selectivity of the present invention is strong, just at dragon spruce cone steinernema, natural enemy is not had injury effect, and is nontoxic to people and animals, environmentally safe.
Description of drawings Fig. 1 is that dragon spruce cone steinernema male moth in Inner Mongol is to the feeler electrophysiologic response gas-chromatography-EAG of female moth body of gland extract and reference compound.
Fig. 2 is the anti-of different proportion, and is anti-/ anti-, suitable-8, and 10-dodecylene alcohol acetic ester woodland lures the moth design sketch.
The invention will be further described below in conjunction with accompanying drawing.
The screening compound test
The dragon spruce cone steinernema of the main object of protection with serious harm China Inner Mongol Nature Reserve---sand ground dragon spruce cone is research object, with female moth body of gland extract and 9 kinds of compounds, surveys its feeler electrophysiologic response.
The female moth body of gland of Inner Mongol dragon spruce cone steinernema extract among Fig. 1-A (17 female moth body of gland equivalent) exists two trace components can cause the remarkable electrophysiologic response of male moth feeler, as shown by arrows in the female moth body of gland extract.
Fig. 1-B: reference compound test
Table 1 test compound
Fig. 1-B shows that the gas chromatography-mass spectrography analysis result shows, test 7,8 is namely anti-, and is anti--8,10-dodecylene alcohol acetic ester and anti-, and suitable-8,10 dodecylene alcohol acetic esters cause male moth feeler electrophysiologic response.
By last figure as seen, except above-mentioned two kinds of materials have the significant feeler electrophysiologic activity, corresponding with it test 3 is namely anti-, and anti--8,10 lanolin alcohols also can cause strong tentaculum electric potential.
Woodland is given birth to test and is tested
Table 2 dragon spruce cone steinernema is to two kinds of different isomerization body proportioning woodland reactions of lanolin alcohol
In test A, B, the C combination, anti-, anti-/ anti-, the ratio of suitable-8,10 dodecylene alcohol acetic esters, dosage are all identical, and is anti-, and anti-/ anti-, the dosage of suitable-8,10 lanolin alcohols changes.
Table 3 lanolin alcohol is to the attracting action woodland test of dragon spruce cone steinernema
Continuous table 3
At duration of test, all trapping amounts for examination lure 2334 male moths of dragon spruce cone steinernema altogether, wherein, lure the trapping amount of core A, B and C basic identical, the trapping amount is respectively 743,747 and 806 male moths, respectively accounting for the trapping total amount is about 33%, only is 38 male moths and lure the trapping amount of core D, only accounts for 1.63% of trapping total amount.Further statistical results show, A, B compare with D with C, the significant difference of trapping amount (P＜0.05).The result shows: anti-, instead-8, the 10-dodecylene is pure and mild anti-, suitable-8,10 lanolin alcohols do not have attractive activity to Inner Mongol dragon spruce steinernema, many and few trap effect to the dodecylene alcohol acetic ester of above-mentioned two kinds of dosages of substance does not have influence substantially, and above-mentioned acetate esters sex pheromone combination is not had synergistic effect, and it is not the pheromones component of dragon spruce cone steinernema.
Above-mentioned evidence, the sex pheromone of dragon spruce cone steinernema are anti-, anti--8,10-dodecylene alcohol acetic ester and anti-, suitable-8,10 dodecylene alcohol acetic esters, and the open-air woodland trapping test of using the different proportionings of these two kinds of compounds has also demonstrated the identical reaction pattern to male moth.
Different proportionings and the dosetest of two kinds of isomer of dodecylene alcohol acetic ester
The applicant utilizes the different proportionings of two kinds of isomer of dodecylene alcohol acetic ester and dosage (seeing Table 4) that dragon spruce cone steinernema has been carried out woodland trapping test 31 days～June 15 May in 2011 in Inner Mongol white tone pile of stones Nature Reserve, the results are shown in Table 5, Fig. 2.
Different proportionings and the dosage of two kinds of isomer of table 4 dodecylene alcohol acetic ester
Table 5 dragon spruce cone steinernema is to the woodland reaction test of different sex pheromone acetic acid esters proportionings
Fig. 2 is the anti-of different proportion, and is anti-/ anti-, suitable-8, and 10-dodecylene alcohol acetic ester woodland lures the moth effect, and transverse axis A-E represents instead, anti--/instead, and suitable-8, the proportioning of 10-dodecylene alcohol acetic ester, the longitudinal axis is induced moth number+standard deviation (head/group); Letter a-c represents significance between the difference group.
The result shows that two kinds of dodecylene alcohol acetic ester isomer of different proportionings all have attractive activity to dragon spruce cone steinernema, and the woodland experiment lures 3163 of the male moths of dragon spruce cone steinernema altogether.A lures the trapping amount of core maximum, is 672 male worms, accounts for 21.25% of trapping total amount, and D lures the trapping amount of core to take second place.Statistical results show, the trapping amount difference of A, B, C, D and E is not remarkable, lures core A and E, F trap effect significant difference, and is extremely remarkable with G difference, lures core B, C, D, E, F to compare significant difference with G.
As can be seen, anti-from above indoor external survey and trapping test, anti--8,10-dodecylene alcohol acetic ester and anti-, two kinds of isomer of suitable-8,10 dodecylene alcohol acetic esters have constituted dragon spruce cone steinernema sex pheromone, can be used in population monitoring and the control of dragon spruce cone steinernema.In actual applications, in order to reduce the cost of pheromones, can use separately anti-, suitable-8,10-dodecylene alcohol acetic ester.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 0.3 μ g of 10-dodecylene alcohol acetic ester and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is as follows:
Lure the trapper of core to be arranged on 2 meters high pine branches of tree crown outer rim with dragon spruce cone steinernema sex pheromone of the present invention is housed, can carry out population monitoring and a large amount of trapping control.
Embodiment 2:
Will be anti-, suitable-8, the n-hexane mixing and stirring of 10-dodecylene alcohol acetic ester 0.3 μ g and 100 μ l can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.Its using method is with embodiment 1.
Embodiment 3:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 0.03 μ g of 10-dodecylene alcohol acetic ester and 0.27 μ g and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 1.
Embodiment 4:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 0.075 μ g of 10-dodecylene alcohol acetic ester and 0.225 μ g and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 1.
Embodiment 5:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 0.15 μ g of 10-dodecylene alcohol acetic ester and 0.15 μ g and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 1.
Embodiment 6:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 0.225 μ g of 10-dodecylene alcohol acetic ester and 0.075 μ g and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 1.
Embodiment 7:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 1.5mg of 10-dodecylene alcohol acetic ester and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is as follows:
Lure core to be arranged on the pine branch of tree crown middle and upper part with dragon spruce cone steinernema sex pheromone of the present invention is housed, can disturb the mating control.
Embodiment 8:
Will be anti-, instead-/anti-, suitable-8, the n-hexane mixing and stirring of each 2mg of 10-dodecylene alcohol acetic ester and 100 μ l, can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 7.
Embodiment 9:
Will be anti-, suitable-8, the n-hexane mixing and stirring of 10-dodecylene alcohol acetic ester 1.5mg and 100 μ l can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 7.
Embodiment 10:
Will be anti-, suitable-8, the n-hexane mixing and stirring of 10-dodecylene alcohol acetic ester 2mg and 100 μ l can make dragon spruce cone steinernema sex pheromone solution of the present invention, the sex pheromone solution that makes is splashed into to make in the rubber stopper formula release vehicle lure core.
Its using method is with embodiment 7.
Embodiment 11:
Organic solvent-normal hexane among above-mentioned all embodiment can use carrene, ether, acetone to replace, and makes the corresponding core that lures of the present invention.
Its using method is with embodiment 1 or 7.
Claims (5)
1. dragon spruce cone steinernema sex pheromone is characterized in that: its active ingredient is by instead, and is anti--8,10-dodecylene alcohol acetic ester and anti-, and suitable-8,10 dodecylene alcohol acetic esters are formed.
2. dragon spruce cone steinernema sex pheromone according to claim 1, it is characterized in that: its active ingredient is:
Instead, anti--8,0～0.3 part of 10-dodecylene alcohol acetic ester, anti-, 0.08～0.3 part of suitable-8,10 dodecylene alcohol acetic ester.
3. the preparation method of the described dragon spruce cone of claim 1 steinernema sex pheromone, it is characterized in that: its effective ingredient is dissolved in the organic solvent.
4. the preparation method of dragon spruce cone steinernema sex pheromone according to claim 4 is characterized in that: its organic solvent is selected from a kind of in the following material: n-hexane, carrene, ether, acetone.
5. the described dragon spruce cone of claim 1 steinernema sex pheromone is characterized in that: the application in monitoring, trapping and interference mating control dragon spruce cone steinernema.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210059173 CN102613178B (en) | 2012-03-07 | 2012-03-07 | Cydia strobilella sex pheromone and preparation method thereof |
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| CN 201210059173 CN102613178B (en) | 2012-03-07 | 2012-03-07 | Cydia strobilella sex pheromone and preparation method thereof |
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| CN102613178A CN102613178A (en) | 2012-08-01 |
| CN102613178B true CN102613178B (en) | 2013-09-25 |
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| CN104488863B (en) * | 2014-12-17 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Application method of sex attractant for melia azedarach cryptophlebia ombrodelta lower |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3845108A (en) * | 1973-08-31 | 1974-10-29 | W Roelofs | Trans-7-cis-9-dodecadien-1-yl acetate |
| US4014946A (en) * | 1974-11-25 | 1977-03-29 | Zoecon Corporation | Synthesis of 1-bromonon-4-en-6-yne |
| CN1171196A (en) * | 1996-07-19 | 1998-01-28 | 中国科学院动物研究所 | Efficient sex attractant for scholartree olethreutree |
| CN101147482A (en) * | 2007-11-20 | 2008-03-26 | 北京林业大学 | Sex attractant for holcocerus artemisiae |
-
2012
- 2012-03-07 CN CN 201210059173 patent/CN102613178B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3845108A (en) * | 1973-08-31 | 1974-10-29 | W Roelofs | Trans-7-cis-9-dodecadien-1-yl acetate |
| US4014946A (en) * | 1974-11-25 | 1977-03-29 | Zoecon Corporation | Synthesis of 1-bromonon-4-en-6-yne |
| CN1171196A (en) * | 1996-07-19 | 1998-01-28 | 中国科学院动物研究所 | Efficient sex attractant for scholartree olethreutree |
| CN101147482A (en) * | 2007-11-20 | 2008-03-26 | 北京林业大学 | Sex attractant for holcocerus artemisiae |
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