CN102605437B - Boehmeria nivea degumming method - Google Patents
Boehmeria nivea degumming method Download PDFInfo
- Publication number
- CN102605437B CN102605437B CN201210102187.7A CN201210102187A CN102605437B CN 102605437 B CN102605437 B CN 102605437B CN 201210102187 A CN201210102187 A CN 201210102187A CN 102605437 B CN102605437 B CN 102605437B
- Authority
- CN
- China
- Prior art keywords
- enzymolysis
- alkaline
- liquid
- ramie
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a boehmeria nivea degumming method, comprising the following steps of: weighing boehmeria nivea, adding the boehmeria nivea into an acid enzyme liquid with a pH value of 4-6, heating to a temperature of 30-60 DEG C, and carrying out enzymolysis, wherein the acid enzyme liquid is a mixture of acid pectinase, acid cellulose and acid protease; and then, adding the boehmeria nivea into an alkaline enzyme liquid with the pH value of 7-10, carrying out the enzymolysis, and heating to the temperature of 30-60 DEG C, wherein the enzyme liquid is an alkaline mixture of alkaline pectinase, alkaline cellulose and alkaline protease. The method disclosed by the invention is used for processing the boehmeria nivea and has the advantages of high efficiency, low cost, less energy consumption, less environmental pollution and the like.
Description
Technical field
The invention belongs to ramie manufacture field, be specifically related to the method for China grass degumming.
Background technology
Ramie (
boehmeria nivea) be Urticaceae perennial root, herbaceos perennial, originates in China, is the national treasure of China.China's ramie output accounts for 90% of whole world ramie output, contains the fiber that accounts for ramie weight 70% in ramie, and its fiber is long, intensity is large, is the important source material in textile industry.Ramie processing comprises binding, and dress cage, comes unstuck, and washes fiber crops, rinsing, and dehydration, stain oil, de-oiled water, trembles fiber crops, oven dry waits processing step.Owing to containing the colloid being comprised of pectin, hemicellulose and lignin in ramee, so the step of coming unstuck in ramie processing is the most key, and degumming tech directly affects the quality of fiber.The ramee chemical degumming technique that adopts for a long time strong acid, highly basic, high temperature, high pressure boiling-off, production cost is high, and environmental pollution is serious.The research of ramie biological degumming technology is important measures and the technological development direction that China grass degumming is energy-conservation, lower consumption, reduce discharging with application, application number is that 200710114687.1 Chinese patent application (applying date is on June 10th, 2007) discloses complex enzyme formulation and its preparation method and application for China grass degumming, wherein point out with alkaline pectase, dextranase, neutral proteinase, zytase and mannase at degumming process, by all enzymes enzyme digestion reaction under identical conditions, can not meet all enzymes in high activity, cause enzymolysis efficiency low; And most of enzyme deactivation in enzymolysis, so enzymolysis liquid can not be reused.
Therefore, be badly in need of a kind of method of China grass degumming, during enzymolysis, activity is high, and enzymolysis is abundant, and enzymolysis also can reuse.
Summary of the invention
In view of this, one of object of the present invention is to provide the method for China grass degumming, adopts two step enzymatic isolation methods, makes enzyme enzymolysis under optimum reaction condition, and enzymolysis efficiency is high.
For achieving the above object, technical scheme is:
The method of China grass degumming, concrete steps are as follows:
A. take ramie, putting into pH is the acidicenzym liquid of 4-6, and temperature rises to 30-60 ℃, enzymolysis; Described acidicenzym liquid is acid pectase, the mixture of acidic cellulase and acid protease;
B. steps A gained ramie is put into the alkaline enzyme liquid that pH is 7-10, temperature rises to 30-60 ℃, enzymolysis; Described alkaline enzyme liquid is alkaline pectase, the mixture of alkali cellulose enzyme and alkali protease.
Preferably, in described steps A, putting into pH and be 5 acidicenzym liquid, is enzymolysis under 50 ℃ of conditions in temperature.
Preferably, in described steps A, the weight of acid enzymolysis liquid is equivalent to 2-10 times of ramie weight, and enzymolysis time is 2-8 hour.
Preferred, described acidicenzym liquid is for containing acid pectase, acidic cellulase and the acidic protein enzymatic mixture that mass fraction is 1-2%.
Most preferred, acid pectase described in described acidicenzym liquid, the weight ratio of acidic cellulase and acid protease is 3:1:1.
Preferably, in described step B, putting into pH and be 5 alkaline enzyme liquid, is enzymolysis under 50 ℃ of conditions in temperature.
Preferably, in described step B, the weight of alkaline enzymolysis liquid is equivalent to 2-10 times of ramie weight, and enzymolysis time is 2-8 hour.
Preferred, described alkaline enzyme liquid is for containing alkaline pectase, alkali cellulose enzyme and the basic protein enzymatic mixture that mass fraction is 1-2%.
Most preferred, alkaline pectase described in described alkaline enzyme liquid, alkali cellulose enzyme and alkali protease weight ratio are 3:1:1.
Beneficial effect of the present invention is: the present invention adopts two step enzymatic isolation methods to carry out enzymolysis, acid pectase, and acidic cellulase and acid protease be enzymolysis under acid condition, and alkaline pectase, alkali cellulose enzyme and alkali protease enzymolysis under alkali condition; In enzymolysis, all enzymes are all in optimum reaction condition, and enzymolysis efficiency is high, has shortened enzymolysis time, has improved production efficiency, the abundant enzymolysis of colloid in ramie; Utilize method disclosed by the invention to come unstuck, enzyme is difficult for inactivation, and the enzyme after using in acid enzymolysis liquid and alkaline enzymolysis liquid is lived high, enzymolysis liquid can be reused, reduced the discharge of sewage, reduced the pollution to environment, by method of the present invention, come unstuck and can reduce the infringement to ramee.The degummed ramie specific targets that make by method disclosed by the invention are: residual gum content 2.51-2.65%, fibre strength 4.82-5.02%, runner rate 7.38-8.01%, oil content 1.17-1.23%, good product quality.
The specific embodiment
Below with reference to embodiment, the present invention is described in detail.The experimental technique of unreceipted actual conditions in preferred embodiment, conventionally according to normal condition.
embodiment 1
The method of China grass degumming, concrete steps are:
A. take ramie, put into and be equivalent to the acidicenzym liquid that the pH of 10 times of ramie weight is 4, temperature rises to 30 ℃, enzymolysis 2 hours, acidicenzym liquid is that to contain mass fraction be 1% acid pectase, acidic cellulase and acidic protein enzymatic mixture, acid pectase wherein, acidic cellulase and acid protease weight ratio are 3:1:1;
B. steps A gained ramie is put into and be equivalent to the alkaline enzyme liquid that the pH of 10 times of ramie weight is 7, temperature rises to 30 ℃, enzymolysis 2 hours, alkalescence enzyme liquid is 1% alkaline pectase for containing mass fraction, alkali cellulose enzyme and basic protein enzymatic mixture, alkaline pectase wherein, alkali cellulose enzyme and alkali protease weight ratio 3:1:1.
The degummed ramie specific targets that make in the present embodiment are: residual gum content 2.65%, fibre strength 4.82%, runner rate 8.01%, oil content 1.23%.
embodiment 2
The method of China grass degumming, concrete steps are:
A. take ramie, put into and be equivalent to the acidicenzym liquid that 4 times of pH of ramie weight are 5, temperature rises to 50 ℃, enzymolysis 4 hours, acidicenzym liquid is that to contain mass fraction be 1.5% acid pectase, acidic cellulase and acidic protein enzymatic mixture, acid pectase wherein, acidic cellulase and acid protease weight ratio are 3:1:1;
B. steps A gained ramie is put into and be equivalent to the alkaline enzyme liquid that the pH of 2 times of ramie weight is 8, temperature rises to 50 ℃, enzymolysis 4 hours, alkalescence enzyme liquid is that to contain mass fraction be 1.5% alkaline pectase, alkali cellulose enzyme and basic protein enzymatic mixture, alkaline pectase wherein, alkali cellulose enzyme and alkali protease weight ratio 3:1:1.
In the present embodiment, after ramie processing, the degummed ramie specific targets that make are: residual gum content 2.58%, fibre strength 4.91%, runner rate 7.82%, oil content 1.21%.
embodiment 3
The method of China grass degumming, concrete steps are:
A. take ramie, put into and be equivalent to the acidicenzym liquid that 2 times of pH of ramie weight are 6, temperature rises to 60 ℃, enzymolysis 8 hours, acidicenzym liquid is that to contain mass fraction be 2% acid pectase, acidic cellulase and acidic protein enzymatic mixture, acid pectase wherein, acidic cellulase and acid protease weight ratio are 3:1:1;
B. steps A gained ramie is put into and be equivalent to the alkaline enzyme liquid that the pH of 2 times of ramie weight is 10, it is 60 ℃ that temperature rises to, enzymolysis 8 hours, alkalescence enzyme liquid is that to contain mass fraction be 2% alkaline pectase, alkali cellulose enzyme and basic protein enzymatic mixture, alkaline pectase wherein, alkali cellulose enzyme and alkali protease weight ratio 3:1:1.
In the present embodiment, after ramie processing, the degummed ramie specific targets that make are: residual gum content 2.57%, fibre strength 5.02%, runner rate 7.88%, oil content 1.17%.
embodiment 4
The method of China grass degumming, concrete steps are:
A. take ramie, put into and be equivalent to the acidicenzym liquid that the pH of 6 times of ramie weight is 6, temperature rises to 40 ℃, enzymolysis 6 hours, acidicenzym liquid is that to contain mass fraction be 2% acid pectase, acidic cellulase and acidic protein enzymatic mixture, acid pectase wherein, acidic cellulase and acid protease weight ratio are 3:1:1;
B. steps A gained ramie is put into and be equivalent to the alkaline enzyme liquid that the pH of 6 times of ramie weight is 10, temperature rises to 40 ℃, enzymolysis 6 hours, alkalescence enzyme liquid is that to contain mass fraction be 2% alkaline pectase, alkali cellulose enzyme and basic protein enzymatic mixture, alkaline pectase wherein, alkali cellulose enzyme and alkali protease weight ratio 3:1:1.
In the present embodiment, after ramie processing, the degummed ramie specific targets that make are: residual gum content 2.51%, fibre strength 5.04%, runner rate 7.38%, oil content 1.19%.
The method of China grass degumming disclosed by the invention, all enzymes can be at enzymolysis under optimum reaction condition, and enzyme is difficult for inactivation, therefore can reuse, and can reduce production costs, and reduce the discharge of industrial wastewater, reduces environmental pollution.
Finally explanation is, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by with reference to the preferred embodiments of the present invention, invention has been described, but those of ordinary skill in the art is to be understood that, can to it, make various changes in the form and details, and not depart from the spirit and scope of the present invention that appended claims limits.
Claims (5)
1. the method for China grass degumming, is characterized in that: concrete steps are as follows:
A. take ramie, putting into pH is the acidicenzym liquid of 4-6, and temperature rises to 30-60 ℃, enzymolysis; Described acidicenzym liquid is for containing the acid pectase that mass fraction is 1-2%, acidic cellulase and acidic protein enzymatic mixture; Described acid pectase, the weight ratio of acidic cellulase and acid protease is 3:1:1;
B. steps A gained ramie is put into the alkaline enzyme liquid that pH is 7-10, temperature rises to 30-60 ℃, enzymolysis; Described alkaline enzyme liquid is for containing the alkaline pectase that mass fraction is 1-2%, alkali cellulose enzyme and basic protein enzymatic mixture; Alkaline pectase described in described alkaline enzyme liquid, alkali cellulose enzyme and alkali protease weight ratio are 3:1:1.
2. the method for China grass degumming according to claim 1, is characterized in that: in described steps A, putting into pH and be 5 acidicenzym liquid, is enzymolysis under 50 ℃ of conditions in temperature.
3. according to the method for China grass degumming described in claim 1 or 2, it is characterized in that: in described steps A, the weight of acid enzymolysis liquid is equivalent to 2-10 times of ramie weight, and enzymolysis time is 2-8 hour.
4. the method for China grass degumming according to claim 1, is characterized in that: in described step B, putting into pH and be 8 alkaline enzyme liquid, is enzymolysis under 50 ℃ of conditions in temperature.
5. the method for China grass degumming according to claim 1, is characterized in that: in described step B, doubly, enzymolysis time is 2-8 hour to the 2-10 that the weight of alkaline enzymolysis liquid is equivalent to ramie weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210102187.7A CN102605437B (en) | 2012-04-10 | 2012-04-10 | Boehmeria nivea degumming method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210102187.7A CN102605437B (en) | 2012-04-10 | 2012-04-10 | Boehmeria nivea degumming method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102605437A CN102605437A (en) | 2012-07-25 |
| CN102605437B true CN102605437B (en) | 2014-04-30 |
Family
ID=46523185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210102187.7A Expired - Fee Related CN102605437B (en) | 2012-04-10 | 2012-04-10 | Boehmeria nivea degumming method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102605437B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110512429A (en) * | 2018-05-21 | 2019-11-29 | 句容市华冠服帽厂 | A kind of absorbing sweat taste removal alternate form fiber inner core recycles preprocess method |
| CN115337328A (en) * | 2022-08-31 | 2022-11-15 | 山东省现代中药研究院有限公司 | Preparation method of medicinal and edible traditional Chinese medicine decoction pieces based on biological enzymolysis technology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1614105A (en) * | 2004-09-25 | 2005-05-11 | 江苏紫荆花纺织科技股份有限公司 | Jute degumming process |
-
2012
- 2012-04-10 CN CN201210102187.7A patent/CN102605437B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1614105A (en) * | 2004-09-25 | 2005-05-11 | 江苏紫荆花纺织科技股份有限公司 | Jute degumming process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102605437A (en) | 2012-07-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhu et al. | Microwave-assisted alkali pre-treatment of wheat straw and its enzymatic hydrolysis | |
| Hideno et al. | Combination of hot compressed water treatment and wet disk milling for high sugar recovery yield in enzymatic hydrolysis of rice straw | |
| CN101451132B (en) | Complex enzyme preparation for China grass degumming as well as preparation method and use thereof | |
| Dutta et al. | Improved production of reducing sugars from rice husk and rice straw using bacterial cellulase and xylanase activated with hydroxyapatite nanoparticles | |
| Almeida et al. | Passion fruit peel as novel substrate for enhanced β-glucosidases production by Penicillium verruculosum: Potential of the crude extract for biomass hydrolysis | |
| CN102134069B (en) | Preparation method of activated carbon | |
| Goyal et al. | Parametric optimization of cultural conditions for carboxymethyl cellulase production using pretreated rice straw by Bacillus sp. 313SI under stationary and shaking conditions | |
| CN105088359B (en) | A kind of brief immersion flax degumming technique | |
| CN102888433B (en) | Method for improving enzymatic hydrolysis efficiency of bamboo reed stalks | |
| Menon et al. | Enzymatic hydrolysis and ethanol production using xyloglucanase and Debaromyces hansenii from tamarind kernel powder: galactoxyloglucan predominant hemicellulose | |
| Guo et al. | Effect of the combined physical and chemical treatments with microbial fermentation on corn straw degradation | |
| CN104498562A (en) | Method for pretreating agricultural waste biomass by adopting sodium hydroxide/urea/water system | |
| CN1831013A (en) | Corn starch soaking prodn. process of adding vegetable protease | |
| Świątek et al. | The improvement of enzymatic hydrolysis efficiency of rape straw and Miscanthus giganteus polysaccharides | |
| CN103665195B (en) | A kind of method utilizing agar in Enzymatic Extraction fragrant plant mentioned in ancient texts | |
| CN103835002A (en) | Method for degumming ambary by low-temperature plasma and biological enzyme | |
| CN102605437B (en) | Boehmeria nivea degumming method | |
| Yan et al. | Oligosaccharide preparation from microwave-ethanol pretreated Camellia oleifera seed shell by enzymolysis of Agrocybe aegerita | |
| CN103665183A (en) | Producing method of sodium alginate with ultralow viscosity | |
| CN105603021A (en) | Method for enhancing tobacco stem enzymolysis effect by using surfactant | |
| Han et al. | Continuous feeding of spent ammonium sulphite liquor improves the production and saccharification performance of cellulase by Penicillium oxalicum | |
| Mund et al. | Chemical composition, pretreatments and saccharification of Senna siamea (Lam.) HS Irwin & Barneby: An efficient biomass producing tree legume | |
| CN106894271A (en) | The quick-fried method with enzymatic hydrogen peroxide oxidation combined pretreatment lignocellulose-like biomass of sulfur trioxide low-grade fever | |
| CN103734748A (en) | Method for extracting dietary fiber from laminaria japonica | |
| CN102787372B (en) | Degumming method for corn cob wrapper fiber and obtained corn cob wrapper fiber |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140430 Termination date: 20150410 |
|
| EXPY | Termination of patent right or utility model |