CN102605052B - Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit - Google Patents
Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit Download PDFInfo
- Publication number
- CN102605052B CN102605052B CN201210033109.6A CN201210033109A CN102605052B CN 102605052 B CN102605052 B CN 102605052B CN 201210033109 A CN201210033109 A CN 201210033109A CN 102605052 B CN102605052 B CN 102605052B
- Authority
- CN
- China
- Prior art keywords
- primer
- amplification
- fluorescence detection
- 235delc
- detection kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 206010011878 Deafness Diseases 0.000 title claims abstract description 19
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 9
- 101000954092 Homo sapiens Gap junction beta-2 protein Proteins 0.000 title abstract description 21
- 208000016354 hearing loss disease Diseases 0.000 title abstract description 7
- 231100000895 deafness Toxicity 0.000 title abstract description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 19
- 230000003321 amplification Effects 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 108010007570 Amelogenin Proteins 0.000 claims abstract description 11
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 238000005251 capillar electrophoresis Methods 0.000 claims abstract description 5
- 239000002777 nucleoside Substances 0.000 claims abstract description 4
- 238000003752 polymerase chain reaction Methods 0.000 claims abstract description 4
- 238000003205 genotyping method Methods 0.000 claims abstract description 3
- 102000007325 Amelogenin Human genes 0.000 claims description 10
- 206010028980 Neoplasm Diseases 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 5
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 claims description 5
- 206010064571 Gene mutation Diseases 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 102100037156 Gap junction beta-2 protein Human genes 0.000 abstract description 13
- 239000000203 mixture Substances 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- -1 deoxyribonucleotide Triphosphates Chemical class 0.000 abstract description 2
- 239000007850 fluorescent dye Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 238000012986 modification Methods 0.000 abstract description 2
- 230000004048 modification Effects 0.000 abstract description 2
- 239000000178 monomer Substances 0.000 abstract description 2
- 108020004707 nucleic acids Proteins 0.000 abstract description 2
- 150000007523 nucleic acids Chemical class 0.000 abstract description 2
- 102000039446 nucleic acids Human genes 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 229910021642 ultra pure water Inorganic materials 0.000 abstract description 2
- 239000012498 ultrapure water Substances 0.000 abstract description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000005547 deoxyribonucleotide Substances 0.000 abstract 1
- 238000001215 fluorescent labelling Methods 0.000 abstract 1
- 229910001629 magnesium chloride Inorganic materials 0.000 abstract 1
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- 235000011178 triphosphate Nutrition 0.000 abstract 1
- 238000000034 method Methods 0.000 description 7
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 238000012408 PCR amplification Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 238000013016 damping Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 230000003416 augmentation Effects 0.000 description 2
- 230000002902 bimodal effect Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108700028369 Alleles Proteins 0.000 description 1
- 101100008044 Caenorhabditis elegans cut-1 gene Proteins 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 101150034593 Gjb2 gene Proteins 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000035606 childbirth Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 238000007886 magnetic bead extraction Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 201000006790 nonsyndromic deafness Diseases 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical group C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210033109.6A CN102605052B (en) | 2012-02-14 | 2012-02-14 | Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210033109.6A CN102605052B (en) | 2012-02-14 | 2012-02-14 | Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102605052A CN102605052A (en) | 2012-07-25 |
CN102605052B true CN102605052B (en) | 2014-01-29 |
Family
ID=46522819
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210033109.6A Expired - Fee Related CN102605052B (en) | 2012-02-14 | 2012-02-14 | Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102605052B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103451300B (en) * | 2013-09-11 | 2016-03-09 | 步迅 | Deaf sick tumor susceptibility gene GJB2 235delC, 299delAT mutation detection kit |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6479235B1 (en) * | 1994-09-30 | 2002-11-12 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
CN1873027A (en) * | 2006-03-30 | 2006-12-06 | 韩东一 | Primer in use for in vitro diagnosing GJB2 mutation of deaf gene of autosomal recessive inheritance in non-syndrome |
-
2012
- 2012-02-14 CN CN201210033109.6A patent/CN102605052B/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN102605052A (en) | 2012-07-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102534031B (en) | High-specificity kit for detecting deafness predisposing genes and uses | |
CN107385064B (en) | Fluorescence labeling composite amplification kit for simultaneously amplifying human autosomal SNP and STR loci and application thereof | |
CN103911452B (en) | Chinese population deaf gene screening kit and application thereof | |
CN102146475B (en) | Kit for detecting southeast Asia deletion alpha-thalassemia | |
CN103757091B (en) | Sudden cardiac death rapid gene detection kit and detection method | |
CN104830852B (en) | One kind detection HLA B*15:The multiple real time fluorescence PCR method of 02 allele | |
CN109097463B (en) | Specific primer probe combination, kit and detection method for detecting HLA-A24: 02 allele | |
CN106939334B (en) | Method for detecting fetal DNA content in plasma of pregnant woman | |
CN106399479A (en) | SNP typing kit used for detecting susceptibility genes of type-II diabetes | |
CN102586433B (en) | Deafness predisposing gene 12S rRNA (ribosomal ribonucleic acid) 1494C>T fluorescence detection kit and application thereof | |
CN103451302A (en) | Deafness susceptible gene mitochondrion 12SrDNA 1555A>G and 1494C>T mutant ratio detection kit | |
CN105441540A (en) | Non-syndromic deafness gene polymorphism detecting kit and application thereof | |
CN107586857A (en) | Nucleic acid, kit and method for the red-black coat color gene of Rapid identification pig | |
CN104293932A (en) | Method for detecting HLA-B * 5801 allele based on real-time fluorescence PCR | |
CN102925560A (en) | Kit and method for detecting mutant alpha-Mediterranean anemia genes through HRM (high resolution melting) method | |
CN102534030B (en) | Kit for jointly detecting four deafness predisposing genes and application thereof | |
CN107653305A (en) | A kind of method that quick detection man cattle Y-chromosome haplotype group is formed | |
CN102605052B (en) | Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit | |
CN102660636A (en) | A fluorescence detection kit for detecting deafness susceptibility gene 12S rRNA 1555A>G and application thereof | |
CN106755422B (en) | Detection method of MEG3 gene SNP related to cattle growth traits and application thereof | |
CN106755530B (en) | MGB probe real-time fluorescence PCR method for detecting HLA-A31: 01 allele and primer probe combination thereof | |
CN103451300B (en) | Deaf sick tumor susceptibility gene GJB2 235delC, 299delAT mutation detection kit | |
CN104232774A (en) | Primers for detecting breast cancer susceptibility gene SNP (single nucleotide polymorphism), fluorescent probes and applications | |
CN103451301B (en) | Deaf sick tumor susceptibility gene SLC26A4 2168A > G, IVS7-2A > G mutation detection kit | |
CN205347395U (en) | Leber heredity optic neuropathy gene detect reagent box |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20120725 Assignee: Baishijia (Shanghai) Medical Technology Co., Ltd. Assignor: Beijing Kelingjinyi Biotechnology Co., Ltd. Contract record no.: 2016230000007 Denomination of invention: Fluorescence detection kit for detecting deafness susceptibility gene GJB2 235delC and application of fluorescence detection kit Granted publication date: 20140129 License type: Exclusive License Record date: 20160408 |
|
LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
EC01 | Cancellation of recordation of patent licensing contract | ||
EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Baishijia (Shanghai) Medical Technology Co., Ltd. Assignor: Beijing Kelingjinyi Biotechnology Co., Ltd. Contract record no.: 2016230000007 Date of cancellation: 20170316 |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170327 Address after: 201200 Shanghai City King Road Chuansha town Pudong District No. 999 Jobon business park 1014-D Patentee after: Wan Gejiang Address before: 100101 Beijing city Chaoyang District Datun Road No. 15 Patentee before: Beijing Kelingjinyi Biotechnology Co., Ltd. |
|
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140129 Termination date: 20200214 |