CN102590170A - Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer - Google Patents
Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer Download PDFInfo
- Publication number
- CN102590170A CN102590170A CN2012100471161A CN201210047116A CN102590170A CN 102590170 A CN102590170 A CN 102590170A CN 2012100471161 A CN2012100471161 A CN 2012100471161A CN 201210047116 A CN201210047116 A CN 201210047116A CN 102590170 A CN102590170 A CN 102590170A
- Authority
- CN
- China
- Prior art keywords
- cdte
- dna
- dna1
- dna2
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a method for simultaneously detecting a divalent mercury ion and a silver ion in water solution based on fluorescence resonance energy transfer, belonging to the field of heavy metal ion detecting technology. The method comprises the following steps: firstly, preparing a detecting probe, comprising following steps: (1), preparing a cadmium telluride (CdTe) quantum dot which is encapsulated by mercaptopropionic acid (MPA); (2), designing and composing a nucleic acid probe for detection; (3), preparing a fluorescent probe CdTe-DNA of quantum dots and nucleic acid; and secondly, detecting heavy metal ion Ag+ and/or Hg2+. The method provided by the invention can detect two ions simultaneously, the workload is low, the cost is low and the process is not complex.
Description
Technical field
The present invention relates to a kind of method, belong to the detection technique field of heavy metal ion based on dimercurion and silver ion in the FRET detection WS.
Background technology
Heavy metal ion such as mercury ion and/or silver ion are the serious environmental pollutants.Their contaminant water and soil cause serious threat and animals and plants are produced serious poisonous effect human health.Mercury ion exists inrichment and long-term contact with it can cause the lasting and serious damage of central nervous system in environment.Silver ion has been proved to be has strong toxicity to hydrobiont.Therefore, invention is a kind of can be effectively and the method for these two kinds of ions of detection of sensitivity is very important.Traditional detection method such as Cold Atomic Fluorescent Mercury spectroscopic methodology (CV-AFS), cold atomic absorption spectrometry (CV-AAS), inductivity coupled plasma mass spectrometry (ICPMS) etc. has a lot of defectives: workload is big, cost is high and process is complicated.In order to solve the problem that traditional detection method brings, develop new sensor and just be necessary very much.FRET (FRET) is a kind of physical phenomenon that was at first proposed before more than 50 years by Theodor F rster.FRET has multiple application, like developed FRET imaging technique and unimolecule FRET detection technique.In recent years, FRET is applied in the detection of many fluorescent materials, as detecting DNA, lysozyme, lemon yellow and histamine.As everyone knows, dimercurion can specificity bonding thymine alkali bases T and is formed T-Hg
2+-T compound, silver ion can specificity bonding cytimidine base C and are formed C-Ag
+-C compound.Up to now, scientists has developed many FRET sensors based on mercury ion and this special nature of silver ion.But these sensors mainly concentrate on a kind of detection of ion, fail to accomplish to detect simultaneously this two kinds of ions.
Summary of the invention
The purpose of this invention is to provide a kind of method of coming to detect simultaneously dimercurion and silver ion in the WS based on FRET.
Technical scheme of the present invention: a kind of method of coming to detect simultaneously dimercurion and silver ion in the WS based on FRET:
(1) preparation of detector probe
(1) preparation of the cadmium telluride CdTe quantum dot of mercaptopropionic acid MPA parcel
With sodium borohydride NaBH
4, ultrapure water and tellurium Te powder be mixed together in the reaction vessel, makes NaBH
4Concentration be 2-3M, the Te powder content is 0.5-0.6M, again reaction vessel is placed the NaHTe solution for standby of 0 ℃ of ice-water bath reaction 12h with the preparation clarification; Take by weighing a certain amount of inorganic cadmium salt Cd
2+Be dissolved in the water control Cd
2+Concentration is 0.01-0.02M, under violent stirring, drips an amount of MPA, and using NaOH solution to regulate pH is 11.20, logical N
2Add NaHTe solution rapidly behind deoxygenation, the protection 30min, keep Cd
2+: Te
2-: the mol ratio of MPA is 1:0.4-0.5:2.2-2.5; Promptly obtain the precursor solution of CdTe quantum dot after the reaction; Get precursor solution and be added to teflon and do in the stainless steel cauldron of liner, 160 ℃ of reaction 20min down promptly obtain needed CdTe quantum dot;
(2) design of the nucleic acid probe of detection usefulness is with synthetic
The single stranded nucleic acid probe DNA1:5'-NH of amido modified mistake
2-GTACAAGATG-3';
Do not make the single stranded nucleic acid probe DNA2:5'-GAGCTTTTCA GACGCATCTT GTACGACTCG CTCCCCATAC-3' of any modification;
The single stranded nucleic acid probe TAMRA-DNA:5'-TAMRA-TTTTGCTC-3' of fluorescent dye TAMRA modified;
The single stranded nucleic acid probe Cy5-DNA:5'-Cy5-GTATCCCC-3' of indoles cyanine dyes Cy5 modified;
(3) preparation of the fluorescence probe CdTe-DNA of quantum dot and nucleic acid
The coupling matter that at first prepares CdTe and DNA1: 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimides EDC; N-hydroxy-succinamide NHS and CdTe solution are admixed together by proper proportion; Activation 0.5-1 h adds the DNA1 WS then, reacts 4h down at 25 ℃; Carry out ultrafiltration with the DNA1 that thorough removal does not join with the CdTe coupling, promptly prepare CdTe-DNA1; Then, at room temperature carry out hybridization reaction 6h to CdTe-DNA1 and DNA2, ultrafiltration promptly prepares probe CdTe-DNA1-DNA2 of quantum dot and DNA to remove the not DNA2 of hybridization; At last, be added to the WS of Cy5-DNA and TAMRA-DNA respectively among the CdTe-DNA1-DNA2 of above-mentioned preparation, promptly prepare the CdTe-DNA fluorescence probe that detects usefulness;
(2) heavy metal ion Ag
+And Hg
2+Detection
(1) Ag
+Detection
Bonding force between silver ion and 3-(N-morpholine) the propane sulfonic acid MOPS very a little less than, so select the MOPS damping fluid as detection architecture: the CdTe-DNA fluorescence probe of preparation in the 100nM step (), 10mM sodium dihydrogen phosphate/sodium hydrogen phosphate NaH
2PO
4/ Na
2HPO
4, the MOPS of 20mM, the sodium nitrate NaNO of 50mM
3
The WS that will contain 0-50 nM variable concentrations silver ion joins in the above-mentioned detection architecture, and under 25 ℃, keeps 0.5h, makes Ag
+Cy5-DNA that induces and DNA2 hybridization reaction carry out fully, record the fluorescent emission collection of illustrative plates of whole system then;
(2) Hg
2+Detection
Detection method and step (1) are similar, just with Ag in the step (1)
+Solion changes Hg into
2+Solution; Contain 0-50 nM variable concentrations Hg
2+The WS of ion joins in the above-mentioned detection architecture, and under 25 ℃, keeps 0.5h, makes Hg
2+TAMRA-DNA that induces and DNA2 hybridization reaction carry out fully, measure the fluorescent emission collection of illustrative plates of whole system then;
(3) carry out Ag simultaneously
+And Hg
2+Detection
Detection method and step (1) are similar, just with Ag in the step (1)
+Solion changes Ag into
+And Hg
2+Mixed solution; Contain 0-50 nM variable concentrations Ag
+And Hg
2+The WS join in the above-mentioned detection architecture, and under 25 ℃, keep 0.5h, make Hg
2+The TAMRA-DNA that induces and DNA2 hybridization reaction and Ag
+Cy5-DNA that induces and DNA2 hybridization reaction carry out fully, measure the fluorescent emission collection of illustrative plates of whole system then.
Described a kind of method of coming to detect simultaneously dimercurion and silver ion in the WS based on FRET: the preparation of the fluorescence probe CdTe-DNA of quantum dot and nucleic acid: the coupling matter that at first is preparation CdTe and DNA1: 1-ethyl-3-of 320 μ L 0.1mM (3-dimethylamino-propyl)-carbodiimides EDC; The N-hydroxy-succinamide NHS of 200 μ L, 0.0125 mM and 1280 μ L, 1 μ M CdTe solution are admixed together, activation 0.5-1 h; The DNA1 WS that adds 100 μ L, 10 μ M then reacted 4 hours down at 25 ℃; Carry out ultrafiltration 15min with ultra filtration membrane with 5000rpm then, and resuspended with ultrapure water, ultrafiltration once more so circulates 3 times, thoroughly removes not the DNA1 that joins with the CdTe coupling, promptly prepares CdTe-DNA1;
Then, at room temperature carry out hybridization reaction 6h to the CdTe-DNA1 of 900 μ L, 1 μ M and 100 μ L, 20 μ M DNA2, carry out same ultrafiltration step and remove the not DNA2 of hybridization, promptly prepare CdTe-DNA1-DNA2;
At last, be added to the WS of 100 μ L, 20 μ M Cy5-DNA and 100 μ L, 20 μ M TAMRA-DNA respectively among the CdTe-DNA1-DNA2 of above-mentioned preparation, promptly prepare the CdTe-DNA fluorescence probe that detects usefulness.
Beneficial effect of the present invention: the present invention provides a kind of method of coming to detect simultaneously dimercurion and silver ion in the WS based on FRET; The present invention can accomplish to detect simultaneously this two kinds of ions, and workload is little comparatively speaking, cost is not high and process is uncomplicated.
Description of drawings
Fig. 1 the present invention detects the schematic flow sheet of dimercurion and silver ion in the WS simultaneously, wherein, and Ag
+And Hg
2+When existing simultaneously, the energy of quantum dot shifts the dyestuff to correspondence, and FRET takes place.
Fig. 2 the present invention the transmission electron microscope photo of CdTe quantum dot of synthetic mercaptopropionic acid parcel.
Fig. 3 the present invention adopted 1, the CdTe quantum dot, 2, TAMRA, 3, ultraviolet absorpting spectrum (solid line) and the fluorescent emission collection of illustrative plates (dotted line) of Cy5, wherein, excite light wavelength to select 380 nanometers.
Fig. 4 embodiment of the invention 1 detects Ag
+The fluorescent emission collection of illustrative plates, wherein, curve a to f representes the Ag in the solution
+Be respectively 0,5,10,15,20,50nM, excite light wavelength to select 380 nanometers.
Fig. 5 embodiment of the invention 2 detects Hg
2+The fluorescent emission collection of illustrative plates, wherein, curve a to f representes the Hg in the solution
2+Be respectively 0,5,10,15,20,50nM, excite light wavelength to select 380 nanometers.
Fig. 6 embodiment of the invention 3 detects Ag
+And Hg
2+The fluorescent emission collection of illustrative plates, wherein, excite light wavelength to select 380 nanometers.
Embodiment
(1) preparation of the CdTe quantum dot of mercaptopropionic acid MPA parcel
Sodium borohydride NaBH with 0.189g (5mmol)
4Be dissolved in the vial that contains the 2.0mL ultrapure water, add 0.1528g (1.175mmol) tellurium Te powder, on bottle cap, stay an aperture, place 0 ℃ of ice-water bath to react reaction vessel again.Behind the 12h, the Te powder of black disappears, the NaHTe solution for standby that obtains clarifying.Take by weighing 0.57g (2.5mmol) CdCl
22.5H
2O is dissolved in the 150mL water, the MPA of Dropwise 5 00 μ L (5.74mmol) under violent stirring, and using the NaOH solution adjusting pH of 0.1mol/L is 11.20, logical N
2Add the above-mentioned NaHTe solution for preparing rapidly behind deoxygenation, the protection 30min, promptly obtain the precursor solution of CdTe quantum dot after the reaction.Get the 40mL precursor solution and be added to teflon and do in the stainless steel cauldron of liner, 160 ℃ of reactions 20 minutes down promptly obtain needed CdTe quantum dot.
(2) design of the nucleic acid probe of detection usefulness is with synthetic
Select the acceptor of 2 kinds of dyestuffs as energy, these 2 kinds of dyestuffs are modified at the 5' end of corresponding nucleic probe respectively; The terminal modified amino of the 5' of another nucleic acid probe is to join in order to have the quantum dot of carboxyl to carry out the covalency coupling with finishing; In addition, also have one to be the nucleic acid probe of not doing modification, as the bridge that connects energy donor quantum dot and energy acceptor dyestuff.All nucleic acid probes are given birth to worker's bioengineering company limited by Shanghai and are synthesized, and its concrete sequence is following:
The single stranded nucleic acid probe DNA1:5'-NH of amido modified mistake
2-GTACAAGATG-3';
Do not make the single stranded nucleic acid probe DNA2:5'-GAGCTTTTCA GACGCATCTT GTACGACTCG CTCCCCATAC-3' of any modification;
The single stranded nucleic acid probe TAMRA-DNA:5'-TAMRA-TTTTGCTC-3' of fluorescent dye TAMRA modified;
The single stranded nucleic acid probe Cy5-DNA:5'-Cy5-GTATCCCC-3' of indoles cyanine dyes Cy5 modified;
(3) fluorescence probe (CdTe-DNA) of preparation quantum dot and nucleic acid
At first be the coupling matter of preparation CdTe and DNA1: 1-ethyl-3-of 320 μ L 0.1mM (3-dimethylamino-propyl)-carbodiimides (EDC); The N-hydroxy-succinamide (NHS) of 200 μ L, 0.0125 mM is admixed together with 1280 μ L, 1 μ M CdTe solution, activation 0.5-1 h.The DNA1 WS that adds 100 μ L, 10 μ M then reacted 4 hours down at 25 ℃.Carry out ultrafiltration 15min with ultra filtration membrane with 5000rpm then, and resuspended with ultrapure water, ultrafiltration once more so circulates 3 times, thoroughly removes not the DNA1 that joins with the CdTe coupling.So promptly prepare CdTe-DNA1.
Next, be 900 μ L concentration that CdTe-DNA1 and 100 μ L, the 20 μ M DNA2 of 1 μ M at room temperature carry out hybridization reaction 6h.Carry out same ultrafiltration step and remove the not DNA2 of hybridization.So promptly prepare CdTe-DNA1-DNA2.
At last, be added to the WS of 100 μ L, 20 μ M Cy5-DNA and 100 μ L, 20 μ M TAMRA-DNA respectively among the CdTe-DNA1-DNA2 of above-mentioned preparation.So promptly prepare the CdTe-DNA fluorescence probe that detects usefulness.
(4) Ag
+Detection
Because the bonding force between silver ion and 3-(N-morpholine) propane sulfonic acid (MOPS) very a little less than, so select the MOPS damping fluid as detection architecture: the CdTe-DNA fluorescence probe of 100nM step () preparation, 10mM sodium dihydrogen phosphate/sodium hydrogen phosphate NaH
2PO
4/ Na
2HPO
4, the MOPS of 20mM, the sodium nitrate NaNO of 50mM
3
The WS that contains variable concentrations (0 to 50 nM) silver ion joins in the above-mentioned detection architecture, and under 25 ℃, keeps 0.5h, and Cy5-DNA and DNA2 hybridization reaction that silver ion is induced carry out fully.Measure the fluorescent emission collection of illustrative plates of whole system then.
(1) preparation of the CdTe quantum dot of mercaptopropionic acid MPA parcel.
Method such as instance 1 are said.
(2) design of the nucleic acid probe of detection usefulness is with synthetic
Method such as instance 1 are said.
(3) fluorescence probe (CdTe-DNA) of preparation quantum dot and nucleic acid
Method such as instance 1 are said.
(4) Hg
2+Detection
Detection architecture such as instance 1 are said, contain variable concentrations (0 to 50 nM) Hg
2+The WS of ion joins in the above-mentioned detection architecture, and under 25 ℃, keeps 0.5h, makes Hg
2+TAMRA-DNA that induces and DNA2 hybridization reaction carry out fully.Measure the fluorescent emission collection of illustrative plates of whole system then.
(1) preparation of the CdTe quantum dot of mercaptopropionic acid MPA parcel.
Method such as instance 1 are said.
(2) design of the nucleic acid probe of detection usefulness is with synthetic
Method such as instance 1 are said.
(3) fluorescence probe (CdTe-DNA) of preparation quantum dot and nucleic acid
Method such as instance 1 are said.
(4) carry out Ag simultaneously
+And Hg
2+Detection
Detection architecture such as instance 1 are said, contain variable concentrations (0 to 50 nM) Ag
+And Hg
2+The WS join in the above-mentioned detection architecture, and under 25 ℃, keep 0.5h, make Hg
2+The TAMRA-DNA that induces and DNA2 hybridization reaction and Ag
+Cy5-DNA that induces and DNA2 hybridization reaction carry out fully.Measure the fluorescent emission collection of illustrative plates of whole system then.
Claims (2)
1. one kind is come to detect simultaneously the method for dimercurion and silver ion in the WS based on FRET, it is characterized in that:
(1) preparation of detector probe
(1) preparation of the cadmium telluride CdTe quantum dot of mercaptopropionic acid MPA parcel
With sodium borohydride NaBH
4, ultrapure water and tellurium Te powder be mixed together in the reaction vessel, makes NaBH
4Concentration be 2-3M, the Te powder content is 0.5-0.6M, again reaction vessel is placed the NaHTe solution for standby of 0 ℃ of ice-water bath reaction 12h with the preparation clarification; Take by weighing a certain amount of inorganic cadmium salt Cd
2+Be dissolved in the water control Cd
2+Concentration is 0.01-0.02M, under violent stirring, drips an amount of MPA, and using NaOH solution to regulate pH is 11.20, logical N
2Add NaHTe solution rapidly behind deoxygenation, the protection 30min, keep Cd
2+: Te
2-: the mol ratio of MPA is 1:0.4-0.5:2.2-2.5; Promptly obtain the precursor solution of CdTe quantum dot after the reaction; Get precursor solution and be added to teflon and do in the stainless steel cauldron of liner, 160 ℃ of reaction 20min down promptly obtain needed CdTe quantum dot;
(2) design of the nucleic acid probe of detection usefulness is with synthetic
The single stranded nucleic acid probe DNA1:5'-NH of amido modified mistake
2-GTACAAGATG-3';
Do not make the single stranded nucleic acid probe DNA2:5'-GAGCTTTTCA GACGCATCTT GTACGACTCG CTCCCCATAC-3' of any modification;
The single stranded nucleic acid probe TAMRA-DNA:5'-TAMRA-TTTTGCTC-3' of fluorescent dye TAMRA modified;
The single stranded nucleic acid probe Cy5-DNA:5'-Cy5-GTATCCCC-3' of indoles cyanine dyes Cy5 modified;
(3) preparation of the fluorescence probe CdTe-DNA of quantum dot and nucleic acid
The coupling matter that at first prepares CdTe and DNA1: 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimides EDC; N-hydroxy-succinamide NHS and CdTe solution are admixed together by proper proportion; Activation 0.5-1 h adds the DNA1 WS then, reacts 4h down at 25 ℃; Carry out ultrafiltration with the DNA1 that thorough removal does not join with the CdTe coupling, promptly prepare CdTe-DNA1; Then, at room temperature carry out hybridization reaction 6h to CdTe-DNA1 and DNA2, ultrafiltration promptly prepares probe CdTe-DNA1-DNA2 of quantum dot and DNA to remove the not DNA2 of hybridization; At last, be added to the WS of Cy5-DNA and TAMRA-DNA respectively among the CdTe-DNA1-DNA2 of above-mentioned preparation, promptly prepare the CdTe-DNA fluorescence probe that detects usefulness;
(2) heavy metal ion Ag
+And/or Hg
2+Detection
(1) Ag
+Detection
Bonding force between silver ion and 3-(N-morpholine) the propane sulfonic acid MOPS very a little less than, so select the MOPS damping fluid as detection architecture: the CdTe-DNA fluorescence probe of preparation in the 100nM step (), 10mM sodium dihydrogen phosphate/sodium hydrogen phosphate NaH
2PO
4/ Na
2HPO
4, the MOPS of 20mM, the sodium nitrate NaNO of 50mM
3
The WS that will contain 0-50 nM variable concentrations silver ion joins in the above-mentioned detection architecture, and under 25 ℃, keeps 0.5h, makes Ag
+Cy5-DNA that induces and DNA2 hybridization reaction carry out fully, record the fluorescent emission collection of illustrative plates of whole system then;
(2) Hg
2+Detection
Detection method and step (1) are similar, just with Ag in the step (1)
+Solion changes Hg into
2+Solution; Contain 0-50 nM variable concentrations Hg
2+The WS of ion joins in the above-mentioned detection architecture, and under 25 ℃, keeps 0.5h, makes Hg
2+TAMRA-DNA that induces and DNA2 hybridization reaction carry out fully, measure the fluorescent emission collection of illustrative plates of whole system then;
(3) carry out Ag simultaneously
+And Hg
2+Detection
Detection method and step (1) are similar, just with Ag in the step (1)
+Solion changes Ag into
+And Hg
2+Mixed solution; Contain 0-50 nM variable concentrations Ag
+And Hg
2+The WS join in the above-mentioned detection architecture, and under 25 ℃, keep 0.5h, make Hg
2+The TAMRA-DNA that induces and DNA2 hybridization reaction and Ag
+Cy5-DNA that induces and DNA2 hybridization reaction carry out fully, measure the fluorescent emission collection of illustrative plates of whole system then.
2. a kind of method of coming to detect simultaneously dimercurion and silver ion in the WS based on FRET according to claim 1; It is characterized in that: the preparation of the fluorescence probe CdTe-DNA of quantum dot and nucleic acid: the coupling matter that at first is preparation CdTe and DNA1: 1-ethyl-3-of 320 μ L 0.1mM (3-dimethylamino-propyl)-carbodiimides EDC; The N-hydroxy-succinamide NHS of 200 μ L, 0.0125 mM and 1280 μ L, 1 μ M CdTe solution are admixed together, activation 0.5-1 h; The DNA1 WS that adds 100 μ L, 10 μ M then reacted 4 hours down at 25 ℃; Carry out ultrafiltration 15min with ultra filtration membrane with 5000rpm then, and resuspended with ultrapure water, ultrafiltration once more so circulates 3 times, thoroughly removes not the DNA1 that joins with the CdTe coupling, promptly prepares CdTe-DNA1;
Then, at room temperature carry out hybridization reaction 6h to the CdTe-DNA1 of 900 μ L, 1 μ M and 100 μ L, 20 μ M DNA2, carry out same ultrafiltration step and remove the not DNA2 of hybridization, promptly prepare CdTe-DNA1-DNA2;
At last, be added to the WS of 100 μ L, 20 μ M Cy5-DNA and 100 μ L, 20 μ M TAMRA-DNA respectively among the CdTe-DNA1-DNA2 of above-mentioned preparation, promptly prepare the CdTe-DNA fluorescence probe that detects usefulness.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2012100471161A CN102590170B (en) | 2012-02-28 | 2012-02-28 | Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2012100471161A CN102590170B (en) | 2012-02-28 | 2012-02-28 | Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102590170A true CN102590170A (en) | 2012-07-18 |
CN102590170B CN102590170B (en) | 2013-11-20 |
Family
ID=46479110
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2012100471161A Active CN102590170B (en) | 2012-02-28 | 2012-02-28 | Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102590170B (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102879336A (en) * | 2012-09-25 | 2013-01-16 | 江南大学 | Method for manufacturing plasma chiral ligand sensor for mercury ions |
CN103743711A (en) * | 2014-01-01 | 2014-04-23 | 桂林理工大学 | Method for detecting gibberellins in food by using cyclodextrin and fluorescence resonance energy transfer technology |
CN104307376A (en) * | 2014-11-05 | 2015-01-28 | 中国科学院合肥物质科学研究院 | Preparation device for thin film standard applying X-ray fluorescence spectroscopy for heavy metal detection, and application thereof |
CN105115946A (en) * | 2015-07-02 | 2015-12-02 | 长春理工大学 | FRET-based fluorescence detection apparatus |
CN107300543A (en) * | 2017-05-31 | 2017-10-27 | 中南大学 | The method of AgNCs probe super sensitivity detection mercury ion of the one kind based on T Hg (II) T structures |
CN107353903A (en) * | 2017-06-28 | 2017-11-17 | 武汉大学 | A kind of method and its application of synthetic dyestuffs modifying DNA functionalization cadmium content point |
CN109307664A (en) * | 2018-09-30 | 2019-02-05 | 江南大学 | The fluorescent detection probe of metal ion in a kind of detectable living cells |
CN109813887A (en) * | 2019-02-19 | 2019-05-28 | 吉林省爱诺德生物工程有限公司 | A kind of immunological response real-time detection method |
CN110132937A (en) * | 2019-06-05 | 2019-08-16 | 福建师范大学 | A kind of DNA chain detects the preparation method of heavy metal ion as signaling switch |
CN111007053A (en) * | 2020-01-17 | 2020-04-14 | 郑州轻工业大学 | Fluorescent aptamer sensor for detecting silver ion concentration and preparation method and application thereof |
CN111220672A (en) * | 2020-02-15 | 2020-06-02 | 江苏大学 | Preparation method of self-enhanced electrochemiluminescence aptamer sensor for detecting Hg2+ based on energy resonance transfer |
CN111593095A (en) * | 2019-09-30 | 2020-08-28 | 天津大学 | Based on SiO2Nucleic acid probe and hybrid strand signal amplification Ag + detection method |
CN113670887A (en) * | 2021-09-28 | 2021-11-19 | 福州大学 | Method for detecting mercury ions by using fluorescent molecular probe based on nucleic acid aptamer |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060183247A1 (en) * | 2005-02-16 | 2006-08-17 | Korea Advanced Institute Of Science And Technology | Detection method for specific biomolecular interactions using fret between metal nanoparticle and quantum dot |
CN101566625A (en) * | 2009-05-14 | 2009-10-28 | 华东师范大学 | Application of neutral anion fluorescent chemical sensor |
CN101672780A (en) * | 2009-10-15 | 2010-03-17 | 青岛大学 | Analytical method for detecting trace mercury in aqueous medium based on the color change of fluorescence |
CN101706437A (en) * | 2009-10-15 | 2010-05-12 | 青岛大学 | Fluorescent analysis method for detecting trace mercury in water medium |
US20100151579A1 (en) * | 2008-10-10 | 2010-06-17 | Zidong Wang | Fluorescent sensor for mercury |
CN101832935A (en) * | 2009-03-10 | 2010-09-15 | 苏州市长三角系统生物交叉科学研究院有限公司 | Target molecule detection method based on nanometer-gold and nucleic acid structure and kit thereof |
JP2010210250A (en) * | 2009-03-06 | 2010-09-24 | Kanagawa Univ | Detection method of mercury ion and kit |
CN101851500A (en) * | 2010-05-05 | 2010-10-06 | 大连理工大学 | Fluorboric dye fluorescent probe for detecting mercury ions |
CN101936905A (en) * | 2009-07-03 | 2011-01-05 | 烟台海岸带可持续发展研究所 | Mercury ion detection reagent and detection method |
CN102200510A (en) * | 2011-04-13 | 2011-09-28 | 上海出入境检验检疫局机电产品检测技术中心 | Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe |
-
2012
- 2012-02-28 CN CN2012100471161A patent/CN102590170B/en active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060183247A1 (en) * | 2005-02-16 | 2006-08-17 | Korea Advanced Institute Of Science And Technology | Detection method for specific biomolecular interactions using fret between metal nanoparticle and quantum dot |
US20100151579A1 (en) * | 2008-10-10 | 2010-06-17 | Zidong Wang | Fluorescent sensor for mercury |
JP2010210250A (en) * | 2009-03-06 | 2010-09-24 | Kanagawa Univ | Detection method of mercury ion and kit |
CN101832935A (en) * | 2009-03-10 | 2010-09-15 | 苏州市长三角系统生物交叉科学研究院有限公司 | Target molecule detection method based on nanometer-gold and nucleic acid structure and kit thereof |
CN101566625A (en) * | 2009-05-14 | 2009-10-28 | 华东师范大学 | Application of neutral anion fluorescent chemical sensor |
CN101936905A (en) * | 2009-07-03 | 2011-01-05 | 烟台海岸带可持续发展研究所 | Mercury ion detection reagent and detection method |
CN101672780A (en) * | 2009-10-15 | 2010-03-17 | 青岛大学 | Analytical method for detecting trace mercury in aqueous medium based on the color change of fluorescence |
CN101706437A (en) * | 2009-10-15 | 2010-05-12 | 青岛大学 | Fluorescent analysis method for detecting trace mercury in water medium |
CN101851500A (en) * | 2010-05-05 | 2010-10-06 | 大连理工大学 | Fluorboric dye fluorescent probe for detecting mercury ions |
CN102200510A (en) * | 2011-04-13 | 2011-09-28 | 上海出入境检验检疫局机电产品检测技术中心 | Mercuric ion concentration fluorescence detection method based on T-T mismatched DNA probe |
Non-Patent Citations (4)
Title |
---|
AKIRA ONO ET AL.: "Synthesis of Novel Metal Ion Sensors Based on DNA-Metal Interactions", 《复旦学报(自然科学版)》 * |
SANTANU PYNE ET AL.: "FRET based ultra sensor for detection of Hg (II) in water:A comparative study using citrate and marcapto propanoic acid as stabilizer of AuNPs", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
WU HUIWANG ET AL.: "An Oligonucleotide-based Fluorescence Sensor for Mercury(II) in Aqueous Solutions", 《CHINESE JOURNAL OF CHEMISTRY》 * |
ZHENZHEN LIN ET AL.: "Impedimetric Immobilized DNA-Based Sensor for Simultaneous Detection of Pb2+, Ag+, and Hg2+", 《ANALYTICAL CHEMISTRY》 * |
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102879336A (en) * | 2012-09-25 | 2013-01-16 | 江南大学 | Method for manufacturing plasma chiral ligand sensor for mercury ions |
CN103743711A (en) * | 2014-01-01 | 2014-04-23 | 桂林理工大学 | Method for detecting gibberellins in food by using cyclodextrin and fluorescence resonance energy transfer technology |
CN103743711B (en) * | 2014-01-01 | 2015-12-30 | 桂林理工大学 | Utilize the method for gibberellin in cyclodextrin and FRET (fluorescence resonance energy transfer) technology for detection food |
CN104307376A (en) * | 2014-11-05 | 2015-01-28 | 中国科学院合肥物质科学研究院 | Preparation device for thin film standard applying X-ray fluorescence spectroscopy for heavy metal detection, and application thereof |
CN105115946A (en) * | 2015-07-02 | 2015-12-02 | 长春理工大学 | FRET-based fluorescence detection apparatus |
CN107300543A (en) * | 2017-05-31 | 2017-10-27 | 中南大学 | The method of AgNCs probe super sensitivity detection mercury ion of the one kind based on T Hg (II) T structures |
CN107300543B (en) * | 2017-05-31 | 2019-11-19 | 中南大学 | A method of being based on the AgNCs probe super sensitivity detection mercury ion of T-Hg (II)-T structure |
CN107353903B (en) * | 2017-06-28 | 2019-05-10 | 武汉大学 | A kind of method and its application of synthetic dyestuffs modifying DNA functionalization cadmium content point |
CN107353903A (en) * | 2017-06-28 | 2017-11-17 | 武汉大学 | A kind of method and its application of synthetic dyestuffs modifying DNA functionalization cadmium content point |
CN109307664A (en) * | 2018-09-30 | 2019-02-05 | 江南大学 | The fluorescent detection probe of metal ion in a kind of detectable living cells |
CN109307664B (en) * | 2018-09-30 | 2020-06-09 | 江南大学 | Fluorescent detection probe capable of detecting metal ions in living cells |
CN109813887A (en) * | 2019-02-19 | 2019-05-28 | 吉林省爱诺德生物工程有限公司 | A kind of immunological response real-time detection method |
CN110132937A (en) * | 2019-06-05 | 2019-08-16 | 福建师范大学 | A kind of DNA chain detects the preparation method of heavy metal ion as signaling switch |
CN111593095A (en) * | 2019-09-30 | 2020-08-28 | 天津大学 | Based on SiO2Nucleic acid probe and hybrid strand signal amplification Ag + detection method |
CN111593095B (en) * | 2019-09-30 | 2023-04-18 | 天津大学 | Based on SiO 2 Nucleic acid probe and hybrid strand signal amplification Ag + detection method |
CN111007053A (en) * | 2020-01-17 | 2020-04-14 | 郑州轻工业大学 | Fluorescent aptamer sensor for detecting silver ion concentration and preparation method and application thereof |
CN111007053B (en) * | 2020-01-17 | 2020-12-08 | 郑州轻工业大学 | Fluorescent aptamer sensor for detecting silver ion concentration and preparation method and application thereof |
CN111220672A (en) * | 2020-02-15 | 2020-06-02 | 江苏大学 | Preparation method of self-enhanced electrochemiluminescence aptamer sensor for detecting Hg2+ based on energy resonance transfer |
CN113670887A (en) * | 2021-09-28 | 2021-11-19 | 福州大学 | Method for detecting mercury ions by using fluorescent molecular probe based on nucleic acid aptamer |
Also Published As
Publication number | Publication date |
---|---|
CN102590170B (en) | 2013-11-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102590170B (en) | Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer | |
Zhang et al. | DNAzyme-triggered visual and ratiometric electrochemiluminescence dual-readout assay for Pb (II) based on an assembled paper device | |
Singh et al. | Nanomaterial-based fluorescent sensors for the detection of lead ions | |
Li et al. | In situ incorporation of fluorophores in zeolitic imidazolate framework-8 (ZIF-8) for ratio-dependent detecting a biomarker of anthrax spores | |
Chang et al. | Synthesis of yellow fluorescent carbon dots and their application to the determination of chromium (III) with selectivity improved by pH tuning | |
You et al. | Superhydrophobic silica aerogels encapsulated fluorescent perovskite quantum dots for reversible sensing of SO2 in a 3D-printed gas cell | |
Yang et al. | One stone, two birds: pH-and temperature-sensitive nitrogen-doped carbon dots for multiple anticounterfeiting and multiple cell imaging | |
Shu et al. | Incorporation of perovskite nanocrystals into lanthanide metal-organic frameworks with enhanced stability for ratiometric and visual sensing of mercury in aqueous solution | |
Huang et al. | Colorimetric determination of uranyl (UO22+) in seawater via DNAzyme-modulated photosensitization | |
Ju et al. | based biodetection using luminescent nanoparticles | |
Liu et al. | Fast and efficient “on-off-on” fluorescent sensor from N-doped carbon dots for detection of mercury and iodine ions in environmental water | |
Šafranko et al. | An overview of the recent developments in carbon quantum dots—promising nanomaterials for metal ion detection and (bio) molecule sensing | |
Tang et al. | A smartphone-integrated optical sensing platform based on Lycium ruthenicum derived carbon dots for real-time detection of Ag+ | |
CN104359880A (en) | Chemical preparation method for CdTe quantum dot fluorescent probe for detecting trace amount of paraquat | |
Li et al. | A novel fluorescent probe involving a graphene quantum dot–enzyme hybrid system for the analysis of hydroquinone in the presence of toxic resorcinol and catechol | |
Zhou et al. | Chemical-tongue sensor array for determination of multiple metal ions based on trichromatic lanthanide-based nanomaterials | |
Qiao et al. | Integration of black phosphorus and hollow-core anti-resonant fiber enables two-order magnitude enhancement of sensitivity for bisphenol A detection | |
Zhang et al. | A label-free fluorescent molecular switch for Cu 2+ based on metal ion-triggered DNA-cleaving DNAzyme and DNA intercalator | |
Li et al. | H2O2‐and pH‐sensitive CdTe quantum dots as fluorescence probes for the detection of glucose | |
Luo et al. | A highly selective fluorescence sensing platform for nanomolar Hg (II) detection based on cytosine derived quantum dot | |
Chen et al. | Label-free iodide detection using functionalized carbon nanodots as fluorescent probes | |
Wang et al. | Recent developments of the speciation analysis methods for silver nanoparticles and silver ions based on atomic spectrometry | |
Nan et al. | An inner-filter-effect based ratiometric fluorescent sensor for the detection of uranyl ions in real samples | |
Guo et al. | Ratiometric fluorescent test paper based on silicon nanocrystals and carbon dots for sensitive determination of mercuric ions | |
Wen et al. | Y-type DNA structure stabilized p-type CuS quantum dots to quench photocurrent of ternary heterostructure for sensitive photoelectrochemical detection of miRNA |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: 214016 Jiangsu city of Wuxi Province District Liangxi No. 898 South Road 7 layer beam Creek area of food science and Technology Park Patentee after: Jiangnan University Address before: Food College of Jiangnan University No. 1800 214122 Jiangsu city of Wuxi province Wuxi City Binhu Lihu Avenue Patentee before: Jiangnan University |