CN102579494A - Pig bone extract, preparation method and application thereof - Google Patents
Pig bone extract, preparation method and application thereof Download PDFInfo
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- CN102579494A CN102579494A CN2011100202847A CN201110020284A CN102579494A CN 102579494 A CN102579494 A CN 102579494A CN 2011100202847 A CN2011100202847 A CN 2011100202847A CN 201110020284 A CN201110020284 A CN 201110020284A CN 102579494 A CN102579494 A CN 102579494A
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Abstract
The invention relates to a pig bone extract, a preparation method and an application thereof. The pig bone extract is a mixture chelated by protein enzymolyzed amino acid, micromolecule polypeptide, and inorganic elements, wherein the protein enzymolyzed amino acid and micromolecule polypeptide are extracted from pig bone raw materials, and the inorganic elements includes at least calcium. The pig bone extract comprises, by content of N, protein, polypeptide and amino acid with a total content not lower than 13w%, and calcium with a content not lower than 2w%, wherein the proteolysis rate>=18%, chelation rate of calcium >=75%. The preparation method includes subjecting the smashed extract of the pig bone raw materials to a solid-liquid separation, subjecting aqueous protein in the liquid to an enzymolysis, subjecting bone residues to an acidolysis, and subjecting the enzymolysis liquid and the acidolysis liquid to a chelation reaction so as to obtain the extract. The pig bone extract can be processed into drugs or health-care food which are capable of improving the immunologic function combined with acceptable auxiliary elements in pharmacy or food industry.
Description
Technical field
The present invention relates to a kind of Os Sus domestica abstract.
Background technology
The weight that comprises the poultry by-product of skeleton often can account for the 30-40% of livestock body weight.At present still insufficient to the utilization of a large amount of poultry skeletons, both wasted the rational resource of this abundant nutrients and ratio, on the problem that bone is handled, polluted environment again, bring certain ambient pressure.
Research shows that the composition that contains in the fowl poultry bone not only contains necessary trace element such as the necessary calcium of human body, phosphorus, also contains nutritional labelings such as rich in protein, fat simultaneously.Main path to bright bone processing has at present: the freezing and pulverizing method is processed fresh bone paste; Or through high temperature, high-pressure water boiling Hydrolyze method, room temperature, water at atmospheric pressure solution to boil water, acid hydrolysis or alkali hydrolysis method; Approach such as enzyme hydrolysis method extract the bone hydrolysis nutritional labelings such as collagen protein, aminoacid, fat.The albumen, polypeptide, the amino acids product that make through said method; Mainly comprise osseocolla, ossein etc.; Contain comparatively rich in protein, polypeptide and aminoacid in the extract; Wherein mainly higher with glycine, hydroxyproline and alanine equal size, can be used as the body important protein and replenish the source.But producing at present often needs to use some chemical reagent or through long-time HTHP heating, causes some unstable aminoacid to be destroyed easily in the process of osseocolla, ossein etc., makes that nutritional labeling is difficult to equilibrium in the product.In addition,, cause the product mouthfeel relatively poor, have tangible astringent taste, bitterness though materials such as the acid that adds in process of production, alkali can not be removed its harmful effect fully through post processing.Recently, enzymatic hydrolysis bone protein technology has obtained bigger development, but in application process, adopts single enzymolysis usually, or the multienzyme fractional hydrolysis, make the Proteolytic enzyme rate not high, or the response time is long, and production cost is too high.
On the other hand, inorganic calcium salt, traditional organic calcium salt and novel soluble organic calcium three phases have been gone through in the development of calcium preparation.The initial simple mixtures of using such as inorganic oxide calcium are prone to moisture absorption caking, and unsuitable mixing have certain destruction to supporting one's family, and palatability, trophism and absorbability is poor, causes its biological value lower.Organic salt such as calcium citrate, calcium lactate calcium additive, though can improve the trace elements absorbed rate, its dissolubility is bigger, is prone to dissolve lose, price is higher.Recently, used amino-acid trace element sequestration thing, melted aminoacid and trace element in one, aminoacid is the base stock of protein synthesis, can be reached for body and replenish the additional again amino acid whose dual purpose of calcium.
Summary of the invention
Given this, it is the Os Sus domestica abstract that feedstock production obtains with depleted Os Sus domestica that the present invention at first provides a kind of, and a kind of method for preparing of said this abstract further also is provided, and the application of this abstract.
Os Sus domestica abstract of the present invention; It is the mixture that extracts protein digestion aminoacid and the micromolecule polypeptide that obtains and form the inorganic elements chelating that comprises calcium constituent at least by the Os Sus domestica raw material; Contain in its composition: albumen, polypeptide, total amino acid content with the N cubage are not less than 13w%; Calcium content is not less than 2w%, Proteolytic enzyme rate >=18% wherein, the chelation percent of calcium >=75%.Wherein, mainly calcium in the said inorganic elements, and other elements such as zinc, ferrum of very small amount or trace.
In the above-mentioned Os Sus domestica abstract of the present invention, the inorganic elements of said chelating attitude is a calcium constituent.
The above-mentioned Os Sus domestica abstract of the present invention can prepare by following procedure:
After a, the raw material Os Sus domestica after will pulverizing fully extract with decocting in water, solid-liquid separation.
Liquid after b, the solid-liquid separation partly carries out oil-water separation; In the water layer thing, add the proteolytic enzyme that allows in the food industry; (like conditions such as suitable pH, temperature) is hydrolyzed under the suitable reactive conditions of enzyme, and extremely wherein the percent hydrolysis of protein component >=18% filters and also collects filtrating.
Bone slag after c, the solid-liquid separation after the abundant acidolysis, filters and also collects filtrating in the hydrochloric acid solution of 0.5M～8M.The acidolysis process can be accomplished in common 0.5～10 hour.
After d, the filtrating that two steps of above-mentioned b, c are collected merge, under pH 7.5～8.5 and 45 ℃～55 ℃ conditions, carry out chelatropic reaction, chelation percent >=75% of calcium filters to the reaction system, and the concentrate drying of filtrating obtains said Os Sus domestica chelate.
In the step a of above-mentioned preparation, more little as the pulverized particles of raw material Os Sus domestica, wherein proteic dissolution is high more, and dissolution rate is also fast more.Result of the test shows, generally speaking, select particle diameter be 1～5 centimetre comparatively moderate.Though prolong extraction time, and/or improve and extract temperature, and the solid-liquid ratio that reduces bone and water, can improve extraction ratio, overlong time, and/or temperature is too high, is prone to make partial amino-acid to be damaged, and increases energy consumption simultaneously, strengthens cost.Therefore generally speaking, decocting in water can be accomplished leaching process in 0.5～24 hour.
As a kind of preferred extracting mode, the weight ratio that can take raw material Os Sus domestica and water is 1: (2～4), under pressurized conditions, extracted 3～5 hours in 108 ℃～121 ℃ decocting in water.
Method for preparing b said proteolytic enzyme in the step; Can be chosen in the food industry the various enzymes that can be hydrolyzed to protein that allow to use, at least a in the middle of papain for example commonly used, pepsin, trypsin, Flavourzyme, Alcalase, the Protamex etc.Test shows, generally speaking, during to proteinic hydrolysis, uses the effect of the compound enzyme of being made up of at least two kinds of enzymes, can be superior to only using the effect of single enzyme.
For example, a kind of preferred mode can adopt by containing commercial enzyme Flavourzyme and Protamex that inside/outside cuts enzyme simultaneously with weight 1: (0.5～2) blended compound enzyme carries out proteolysis.In the case, this enzymolysis process can be at the said compound enzyme that adds substrate weight 0.05%～0.15%, and the enzymolysis hydrolysis can be accomplished in 1～10 hour under the condition of and pH5.5～6.5 and 48 ℃～52 ℃.
The chelatropic reaction in said d step in the preparation process can be undertaken by the mode of the products such as calcium amino acid chelate that have report at present and/or use.Wherein, a kind of preferred mode, can adopt filtrating that two steps of above-mentioned b, c are obtained with volume ratio (1～3): 1 mix after, under pH 7.5～8.5 and 45 ℃～55 ℃ conditions, carry out.Chelatropic reaction can be accomplished in 0.5～24 hour usually.
Because Os Sus domestica chelate of the present invention is through suitable restriction endonuclease and/or excision enzyme; Particularly adopt corresponding composite multifunction protease; Can be by obtaining a large amount of free amino acid and micromolecule polypeptide in the Sus domestica extract; For good basis is established in the production of bone chelate complex, and help controlling production cost.Simultaneously; From the bone slag, proposed wherein effective, economic and abundant calcium constituent again,, thereby need not to add chemical calcium source with this calcium source as chelating; Not only help having met natural, green consumption notion, tradition can be fully utilized by exhausted fowl poultry bone to greatest extent.Existing research shows, comprises the trace element of calcium and the complex that aminoacid forms micromolecule dipeptides form, absorption, transhipment and stable aspect, have more advantage than calcium amino acid chelate, more be prone to directly absorbed through the small intestinal approach, improve absorbance greatly.Therefore, be raw material with the above-mentioned Os Sus domestica chelate of the present invention, with acceptable auxiliary element in pharmacy or the food industry, can co-production become to have medicine or the health food that improves immunologic function.
Below in conjunction with the specific embodiment, foregoing of the present invention is remake further detailed description by the accompanying drawing illustrated embodiment.But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following instance.Do not breaking away under the above-mentioned technological thought situation of the present invention, various replacements or change according to ordinary skill knowledge and customary means are made all should comprise within the scope of the invention.
Description of drawings
Fig. 1 is that different proteolytic enzymes compare the proteic hydrolysis effect of Os Sus domestica in the Os Sus domestica chelate method for preparing of the present invention.
Fig. 2 is that the hydrolysis result of the compound enzyme of Flavourzyme and Protamex different mixing proportion compares.
The specific embodiment
The proteic decocting in water of embodiment 1 Os Sus domestica extracts the step test
The HTHP decocting in water is to extract one of proteic most popular method of animal bone.But long decocting in water time and too high decocting in water temperature can be destroyed the thermally labile acidic amino acid in the protein, cause the uneven and disappearance of nutrition.Therefore, through orthogonal optimization is carried out to Os Sus domestica protein extraction technology in solid-liquid ratio, decocting in water temperature and decocting in water time three aspects.
1. test material
Os Sus domestica: commercial; Other reagent are analytical pure.
2. test method
The 2.5kg Os Sus domestica of cleaning, be broken into the 3-5cm size to be put into. the decocting in water jar adds water to scale by the design solid-liquid ratio and under design decocting in water temperature and time condition, carries out decocting in water.Quadrature factor level and EXPERIMENTAL DESIGN are seen table 1, table 2.After decocting in water was accomplished, elimination bone slag separated bone fat, promptly gets the Os Sus domestica protein extract.Adopt Kjeldahl to measure the total protein content in the extracting solution.
Table 1 Os Sus domestica protein extraction technological factor level
The design of table 2 Os Sus domestica protein extraction engineer testing
3, result of the test
Total protein content in each test group extracting solution is as shown in table 3.Adopt Minitab 15.0 software analysis to show (seeing table 4), to the influence of total protein content in the extracting solution, decocting in water temperature＞solid-liquid ratio＞decocting in water time.Can be found out by variance analysis (seeing table 5), be to investigate index with the total protein content, and solid-liquid ratio and decocting in water temperature two factors are remarkable, and the decocting in water time factor is not remarkable.Therefore, take all factors into consideration, confirm that the Os Sus domestica protein extraction optimizes technology and be from total protein content and production cost two aspects: with solid-liquid ratio 1: 4, decocting in water 3h under 121 ℃ of conditions.After decocting in water was accomplished, elimination bone slag separated bone fat, promptly gets the Os Sus domestica protein extract, and protein concentration is about 22.5g/L in the extracting solution, and Os Sus domestica protein extract total protein (in N) is not less than 13.5% after the spray drying.
Table 3 Os Sus domestica protein extraction engineer testing result
Table 4 average Response Table
Table 5 analysis of variance table
The enzymolysis step test of embodiment 2 Os Sus domestica protein extracts
Protein content reaches 11%-15% in the skeleton, and wherein protein is main with collagen protein, accounts for the 70-80% of total protein content.Collagen protein constitutes the triple helix structure by 3 α polypeptide chains, and its molecular structure is very stable.Simultaneously, the albumen kind is numerous in the bone protein, and the hydrolysis of protease has narrow spectrum characteristics.Therefore, using single protease is difficult to bone protein is hydrolyzed to small-molecular weight polypeptide, aminoacid fully.Therefore, this research is intended and is adopted the complex enzyme hydrolysis technology that Os Sus domestica albumen is hydrolyzed.
(1) complex enzyme formula research
1. test material
Os Sus domestica: commercial; Other reagent are analytical pure.
Flavourzyme, Alcalase, Protamex are available from Novozymes Company, and trypsin, papain are available from the biological company limited of Nanning Pang Bo.Its optimum pH and temperature are following: Flavourzyme, optimum pH 5.0-7.0, optimum temperature 40-55 ℃; Alcalase, optimum pH 7.0-9.0, optimum temperature 40-65 ℃; Protamex, optimum pH 5.5-7.5, optimum temperature 35-60 ℃; Trypsin, optimum pH 7.0-8.0, optimum temperature 45-55 ℃; Papain, optimum pH 6.0-7.5, optimum temperature 50-60 ℃.
Other reagent are homemade analytical pure.
2. test method
2.1 being chosen in the extracting solution that obtains according to aforementioned Os Sus domestica protein extraction technology (protein concentration is about 22.5g/L) of enzyme; Amount with 0.1% (mass volume ratio) is added various enzymes respectively; And under various enzyme optimum pHs and temperature conditions reaction 2,4,6,8h, measure the bone protein percent hydrolysis.
2.2 the selection of compound enzyme proportioning is pressed Flavourzyme and Protamex 2: 1, and 1: 1,1: 2 mixed; Amount with 0.1% (mass volume ratio) is added in the Os Sus domestica protein extract respectively; Under 6.0,50 ℃ of conditions of pH, react 2,4,6,8h, measure the bone protein percent hydrolysis.
2.3 the mensuration of the mensuration free amino acid of Proteolytic enzyme rate adopts formol titration, the mensuration of total nitrogen content adopts Kjeldahl.
Proteolytic enzyme rate (%)=free amino acid nitrogen content/total nitrogen * 100%.
3. result of the test
3.1 the selection of enzyme
5 kinds of enzymes are under optimal condition separately, and are as shown in Figure 1 to the proteic hydrolysis effect of Os Sus domestica.Result of the test shows, along with the prolongation of time, 5 kinds of enzymes all present rising trend to the percent hydrolysis of bone protein, but ascensional range is less behind 6h; Flavourzyme is best to the hydrolysis efficiency of bone protein, all is higher than other enzymes at each time point, and Alcalase and Protamex hydrolysis efficiency take second place, but difference is not obvious, and trypsin and papain effect are worst.Because optimum pH and the Flavourzyme of Alcalase differ greatly, adopt compound enzyme enzymolysis simultaneously for reaching, the purpose with production cost of saving time, so select for use Protamex and Flavourzyme composite, carry out follow-up study.
3.2 the selection of compound enzyme proportioning
Flavourzyme and Protamex were pressed 2: 1, and 1: 1, mixed was carried out enzymolysis in 1: 2.Result of the test is as shown in Figure 2.The result shows that two kinds of composite posthydrolysis efficient of enzyme significantly improve, and is wherein the highest with 1: 1 ratio proportioning hydrolysis efficiency.
(2) optimization of enzymatic hydrolysis condition
1. test material
Os Sus domestica: commercial; Other reagent are analytical pure.
Flavourzyme, Protamex are available from Novozymes Company, and its optimum pH and temperature are following: Flavourzyme, optimum pH 5.0-7.0, optimum temperature 40-55 ℃; Protamex, optimum pH 5.5-7.5, optimum temperature 35-60 ℃.
Other reagent are homemade analytical pure.
2. test method
1), hydrolysis temperature, enzymolysis pH, enzymolysis time be optimized adopts orthogonal design, to enzyme concentration (Flavourzyme: Protamex=1:.Quadrature factor level and EXPERIMENTAL DESIGN are seen table 6, table 7.After enzymolysis is accomplished, adopt formol titration to measure free aminoacid content, adopt Kjeldahl to measure total nitrogen content, calculate the Proteolytic enzyme rate by following formula:
Proteolytic enzyme rate (%)=free amino acid nitrogen content/total nitrogen * 100%.
Table 6 Os Sus domestica proteolysis technological factor level
The design of table 7 Os Sus domestica proteolysis engineer testing
3. result of the test
Under each reaction condition, the bone protein percent hydrolysis is as shown in table 8.Adopt Minitab 15.0 software analysis to show (seeing table 9), to the influence of percent hydrolysis, enzyme concentration＞enzymolysis time＞enzymolysis pH＞hydrolysis temperature.According to orthogonal experiments, confirm that the Os Sus domestica proteolysis optimizes technology and be: with 0.15% ratio add compound enzyme (Flavourzyme: Protamex=1: 1), enzymolysis 6h under 6,52 ℃ of conditions of pH.
Repeated trials is 3 times under this condition, Os Sus domestica Proteolytic enzyme rate average out to 20.1%.
Table 8 Os Sus domestica proteolysis engineer testing result
Table 9 average Response Table
The chelating step test of embodiment 3 Os Sus domestica protein extracts
The bone mineral major part is distributed in the organic matter with the form of unbodied calcium phosphate and crystalline hydroxyapatite.Through the extraction of front to bone protein, destroyed original biological structure, the part mineral element is exposed and be dissolved in the extracting solution.In order to utilize resource more fully, this research is further carried out acidolysis to the bone slag after the protein extraction, makes mineral element strippings as far as possible such as calcium, and and then also carries out chelatropic reaction with the bone protein hydrolysis that the front obtains, the preparation bone chelate complex.
1. test material
Os Sus domestica: commercial; Other reagent are analytical pure.
2. test method
2.1 the every kg bone of the acidolysis of bone slag slag adds in the 1L 1M hydrochloric acid several times, under 100 ℃ of conditions, acts on 60min.Filter and collect filtrating, subsequent use.
2.2 orthogonal design is adopted in the optimization of chelating condition, and bone slag hydrolyzed solution and protein hydrolyzate adding proportion, chelating temperature, chelating pH, chelating time are optimized.Quadrature factor level and EXPERIMENTAL DESIGN are seen table 10, table 11.
Table 10 Os Sus domestica proteolysis technological factor level
The design of table 11 Os Sus domestica proteolysis engineer testing
2.3 the total calcium in the assaying reaction liquid of chelation percent adopts the EDTA method directly to measure; Chelating calcium in the reactant liquor precipitates through dehydrated alcohol, and behind the cyclic washing, is dissolved in distilled water again, adopts the EDTA method to measure.
Chelation percent (%)=chelating attitude calcium content/total calcium content * 100%.
3. result of the test
Under each reaction condition, the calcium chelation percent is as shown in Table 12.Adopt Minitab 15.0 software analysis to show (seeing table 13), to the influence of calcium chelation percent, adding proportion＞chelating pH＞chelating temperature＞chelating time.According to orthogonal experiments, the chelating technology of selected Os Sus domestica chelate is that bone slag hydrolyzed solution and protein hydrolyzate adding proportion are 1: 3, under 8.0,50 ℃ of conditions of pH, react 45min.
Repeated trials is 3 times under this condition, the chelation percent average out to 79.1% of Os Sus domestica chelate.
Table 12 chelating engineer testing result
Table 13 average Response Table
The preparation of embodiment 4 Os Sus domestica abstracts
The 2.5kg Os Sus domestica of cleaning, be broken into the 3-5cm size is put into the decocting in water jar, add water to scale at 1: 4 by solid-liquid ratio, decocting in water 3h under 121 ℃ of conditions.Carry out oil-water separation, water layer is put into retort, heat and the temperature of keeping solution to 52 in the retort ℃ half an hour at least, by 0.15% enzyme liquor ratio add compound enzyme (Flavourzyme: Protamex=1: 1), enzymolysis 6h under 52 ℃ of conditions.Under the agitator continuous stirring, begin enzyme digestion reaction, during keep pH 6.0 through adding HCl or NaOH.Get the bone slag that extracts behind the bone protein, add several times in the 1L 1M hydrochloric acid, under 100 ℃ of conditions, act on 60min by every kg bone slag.Filter and collect filtrating, subsequent use.Heating and keep in the retort solution temperature 50 ℃ of half an hour, is under 1: 3 condition with bone slag hydrolyzed solution and protein hydrolyzate adding proportion, keeps pH 8.0, reacts 45min.The spray-dried powder that is prepared into of reactant liquor.
The preparation of embodiment 5 Os Sus domestica abstracts of the present invention
Prepare the Os Sus domestica chelate according to embodiment 1 method for preparing screening decocting in water condition, enzymatic hydrolysis condition, chelating condition, result of the test is seen Test Example 1-3.
The preparation of embodiment 6 oral liquid formulation food of the present invention, health food or medicine
Raw material: Os Sus domestica abstract 1kg of the present invention or chelatropic reaction afterreaction liquid (getting) by embodiment 1 preparation chelate;
Method for preparing: behind abstract dissolving of the present invention or chelatropic reaction afterreaction liquid and conventional adjuvant (Mel, simple syrup, sorbic acid, benzoic acid etc.) mix homogeneously, packing is sterilized, and processes oral liquid formulation food, health food or the medicine of abstract of the present invention.
The preparation of embodiment 7 abstract granule food of the present invention, health food or medicine
Raw material: abstract powder 1kg of the present invention (getting) by embodiment 1 preparation gained abstract;
Method for preparing: abstract powder of the present invention and conventional adjuvant (water soluble excipient, ethanol etc.) are passed through processes such as granulation, drying, granulate, packing, process abstract granule food of the present invention, health food or medicine.
The preparation of embodiment 8 abstract tablet foods of the present invention, health food or medicine
Raw material: abstract powder 100g of the present invention (getting), starch 60g, lactose 30g, magnesium stearate 10g by embodiment 1 preparation gained abstract.
Method for preparing: with abstract powder of the present invention, starch, lactose mixing, after the conventional method granulation, add the magnesium stearate granulate, tabletting is processed 1000 altogether, obtains every tablet of tablet food, health food or medicine that contains abstract powder 100mg of the present invention.
The preparation of embodiment 9 abstract capsule food of the present invention, health food or medicine
Raw material: abstract powder 1kg of the present invention (getting) by embodiment 1 preparation gained chelate;
Method for preparing: abstract powder of the present invention and conventional adjuvant (water soluble excipient, ethanol etc.) are passed through processes such as granulation, drying, granulate, filling, process abstract capsule food of the present invention, health food or medicine.
Claims (10)
1. Os Sus domestica abstract; It is characterized in that protein digestion aminoacid and micromolecule polypeptide that obtains by the extraction of Os Sus domestica raw material and the mixture that forms the inorganic elements chelating that comprises calcium constituent at least; Contain in its composition: albumen, polypeptide, total amino acid content with the N cubage are not less than 13w%; Calcium content is not less than 2w%, Proteolytic enzyme rate >=18% wherein, the chelation percent of calcium >=75%.
2. Os Sus domestica abstract as claimed in claim 1, the inorganic elements that it is characterized in that said chelating attitude is a calcium constituent.
3. the method for preparing of claim 1 or 2 Os Sus domestica abstract is characterized in that by following procedure:
After a, the raw material Os Sus domestica after will pulverizing fully extract with decocting in water, solid-liquid separation;
Liquid after b, the solid-liquid separation partly carries out oil-water separation, in the water layer thing, adds the proteolytic enzyme that allows in the food industry, under the suitable reactive conditions of enzyme, is hydrolyzed, and extremely wherein the percent hydrolysis of protein component >=18% filters and also collects filtrating;
Bone slag after c, the solid-liquid separation after the abundant acidolysis, filters and also collects filtrating in the hydrochloric acid solution of 0.5M～8M;
After d, the filtrating that two steps of above-mentioned b, c are collected merge, under pH 7.5～8.5 and 45 ℃～55 ℃ conditions, carry out chelatropic reaction, chelation percent >=75% of calcium filters to the reaction system, and the concentrate drying of filtrating obtains said Os Sus domestica chelate.
4. method for preparing as claimed in claim 3 is characterized in that it is to carry out decocting in water behind 1～5 centimetre the granule to extract that the said a raw material Os Sus domestica powder in the step is broken into particle diameter.
5. method for preparing as claimed in claim 4 is characterized in that the weight ratio with raw material Os Sus domestica and water is 1: (2～4), under pressurized conditions, extracted 3～5 hours in 108 ℃～121 ℃ decocting in water.
6. method for preparing as claimed in claim 3 is characterized in that the proteolytic enzyme that allows in the said b food industry in the step is at least a among papain, pepsin, trypsin, Flavourzyme, the Protamex.
7. method for preparing as claimed in claim 6, it is characterized in that the said b proteolytic enzyme in the step for by Flavourzyme and Protamex with weight 1: (0.5～2) blended compound enzyme.
8. method for preparing as claimed in claim 7 is characterized in that said enzymolysis at the said compound enzyme that adds substrate weight 0.05%～0.15%, and enzymolysis is 1～10 hour under the condition of and pH5.5～6.5 and 48 ℃～52 ℃.
9. like the described method for preparing of one of claim 3 to 8, the chelatropic reaction thing that it is characterized in that the said d step is b, the filtrating in two steps of c with volume ratio (1～3): 1 mixture.
10. the application of claim 1 or 2 said Os Sus domestica abstracts, it is characterized in that with pharmacy or food industry in acceptable auxiliary element co-production become to have to improve the medicine or the health food of immunologic function.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103689212A (en) * | 2013-12-27 | 2014-04-02 | 冯启训 | Method for extracting small molecule peptide from fresh animal bone |
| CN104017046A (en) * | 2014-06-23 | 2014-09-03 | 四川省中医药科学院 | Bone protein and extraction method thereof |
| CN107897632A (en) * | 2017-11-10 | 2018-04-13 | 雷笑天 | A kind of preparation method of high-moisture herbage bundle natural fungicidal agent |
-
2011
- 2011-01-18 CN CN2011100202847A patent/CN102579494A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103689212A (en) * | 2013-12-27 | 2014-04-02 | 冯启训 | Method for extracting small molecule peptide from fresh animal bone |
| CN104017046A (en) * | 2014-06-23 | 2014-09-03 | 四川省中医药科学院 | Bone protein and extraction method thereof |
| CN107897632A (en) * | 2017-11-10 | 2018-04-13 | 雷笑天 | A kind of preparation method of high-moisture herbage bundle natural fungicidal agent |
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Application publication date: 20120718 |