CN102577925A - Breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province - Google Patents
Breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province Download PDFInfo
- Publication number
- CN102577925A CN102577925A CN2012100357787A CN201210035778A CN102577925A CN 102577925 A CN102577925 A CN 102577925A CN 2012100357787 A CN2012100357787 A CN 2012100357787A CN 201210035778 A CN201210035778 A CN 201210035778A CN 102577925 A CN102577925 A CN 102577925A
- Authority
- CN
- China
- Prior art keywords
- rice
- individual plant
- gene
- generation
- blast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province, belonging to the field of rice genetic improvement and biological technology application. The breeding method comprises the following steps of: selecting Huai rice variety (containing Pi-ta genes) which is moderately susceptible to rice panicle blast and Nanjing 38 (containing Pi-b genes) which is susceptible to rice panicle blast to prepare a hybrid group; harvesting single strains of F1 generation, F2 generation and F3 generation in a mixing manner, carrying out molecular marker-assisted selection on rice panicle blast-resistant genes Pi-ta and Pi-b from the F4 generation, and polymerizing two resistance genes to obtain a double-gene homozygote with the gene type of Pi-taPi-taPi-bPi-b; and after yield and agronomic trait selection of subsequent generations, breeding a new high-yield rice strain which is stable and consistent in agronomic trait and resistant to rice panicle blast in Jiangsu province till the F6 generation. The breeding method disclosed by the invention not only can be used for rapidly breeding the new rice variety which is resistant to the rice panicle blast, but also is free of a large amount of inoculation and identification, thereby saving the breeding cost.
Description
One, technical field
The present invention relates to a kind of breeding method of cultivating anti-Jiangsu Province panicle blast rice varieties, belong to rice genetic improvement and biological technology application.
Two, background technology
Rice blast is by Pyricularia oryzae (Magnaporthe oryzae; No condition: one of destructive disease of tool on the paddy rice that Pyriculariaoryzae) causes is commonly called as paddy rice " cancer ".All can receive infecting of rice blast pathogen in the paddy rice whole growing process, can be divided into: seedling pest, leaf pest, pulvinus pest, joint pest, panicle blast, grain pest etc. according to the position difference that infects.Wherein the harm of panicle blast is greater than leaf pest and other harm, can cause when serious that No kernels or seeds are gathered, as in a year of scarcity.Rice blast has the countries and regions of paddy rice plantation that generation is all arranged in 85 in the whole world.According to statistics, 1975～1990 years 16 in the period of, the global paddy rice production loss that is caused by rice blast is up to 1.57 hundred million tons.China is every at a distance from 7-10 once fairly large rice blast harm just to take place, and injured area reaches 300-600 ten thousand hm2, ten thousand tons of paddy loss 70-125.Paddy rice is the first cereal crops in Jiangsu Province, and unit yield ranks first in the whole country, and its intermediate keng rice area accounts for more than 80% of rice area.Rice blast also is the main rice disease in Jiangsu Province, and in early 1990s, onset area reaches 166. 67 ten thousand hm2,350,000 tons of the underproduction, and seedling, leaf, panicle blast all have generation, but the potential threat of panicle blast is maximum.Therefore Jiangsu Province reaches 4 grades the direct elimination system of taking to panicle blast in japonica rice new varieties authorization process.
The control of rice blast is mainly controlled disease through chemical control and plantation resistant variety, facts have proved, seed selection with promote the blast resisting kind be address this problem the most effectively, the method for safety and environmental protection.Excavate anti-source and identify that resistant gene is the basis and the prerequisite of cultivating disease-resistant variety.From different disease-resistant varieties, identify about 80 rice blast resistance genes at present,
Pi-b,
Pi-ta,
Pi-d2,
Pi9,
Pi-21Cloned Deng at least 20 blast resistant genes.But except
Pb1The research of outer these rice blast resistance genes all concentrates on the resistance of seedling pest, leaf pest, can not clear and definite its resistance to panicle blast.Selecting the disease-resistant gene of panicle blast is the prerequisite of panicle blast resistive breeding.
We utilize rice blast resistance gene
Pi-ta,
Pi-bFunctional label to Jiangsu Province over the years the japonica rice variety promoted of large tracts of land carry out genotype detection, ins conjunction with panicle blast resistance result, tentatively clear and definite through association analysis
Pi-ta,
Pi-bJoint effect has resistance preferably to Jiangsu Province's japonica rice rice blast especially panicle blast.The evaluation of resistance is the basis in the rice blast resistance breeding, and authentication method mainly contains the artificial infection evaluation and the lesion spontaneous induction is identified.The evaluation of tradition rice anti-rice blast germ plasm resource mainly is the land for growing field crops mist measuring, and this method required time is longer, needs to consume wide variety of materials, and receives such environmental effects bigger.Compare with field identification method, inoculation identifies and can carry out in early days at plant strain growth that not influenced by extraneous climatic factor, it is little to take up room.But in the resistance of kind is identified, also have many problems, like authentication method, evaluation criterion disunity, authentication method is nonstandard, lack of standardization, identifies that efficient is low, and condition is difficult to control etc. in the evaluation, thereby makes the qualification result that draws inaccurate, is difficult to unified
Standard is judged measurement, and then has influenced breeding for disease resistance work.Utilizing gene function marker assisted selection technology is to select according to the genotype that the difference of the sequence of disease-resistant gene design primer own carries out, and utilizes this method to carry out panicle blast resistive breeding work and not only selects accurately, and do not receive time and environmental limitations.Therefore, a kind of efficient transformation of invention and the breeding method of identifying the panicle blast resistant gene in Jiangsu Province's japonica rice panicle blast resistive breeding process, polymerization high yield, many disease-resistant genes are aims of the present invention in one.
Three, summary of the invention
Technical problem:The present invention relates to a kind of breeding method of cultivating anti-Jiangsu Province panicle blast rice varieties; The breeding method that adopts efficient transformation and resistant gene polymerization to select; In one, reducing the breeding cost, shortening breeding cycle is aim of the present invention with high yield, disease-resistant gene polymerization.
Technical scheme:
A kind of breeding method of cultivating anti-Jiangsu Province japonica rice panicle blast new rice variety is characterized in that:
1) select for use in the sense panicle blast high-yield rice kind Huaihe River, Jiangsu rice 10 (
Pi-taPi-tapi-bpi-b) be maternal, sense panicle blast southern round-grained rice 38 (
Pi-tapi-taPi-bPi-b) be male parent preparing hybrid combo;
2) plantation F
1, F
2, F
3, receipts are mixed in ripe back all individual plant;
3) F
4In generation, begin to carry out the rice blast disease-resistant gene
Pi-ta, Pi-bMolecular marker assisted selection, seedling stage, the SDS method was adopted in the extraction of each individual plant DNA:
(1) utilize 2 pairs of allele specific pcr primer molecule to detect rice blast resistance gene
Pi-taExistence:
Right with the Pita primer
Pita-F：AGCAGGTTATAAGCTAGGCC；Pita-R：CTACCAACAAGTTCATCAAA
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, contains if having this individual plant of 1042 bp characteristic bands explanation
Pi-taGene;
Right with the Npita primer
Npita-F：AGCAGGTTATAAGCTAGCTAT；Npita-R：CTACCAACAAGTTCATCAAA
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, does not contain if having this individual plant of 1042 bp characteristic bands explanation
Pi-taGene;
At F
4To amplifying the characteristic band of 1042bp, the Npita primer is the individual plant that can not amplify 1042bp purpose band simultaneously with the Pita primer in selection in generation
Pi-taThe individual plant of gene pure;
(2) utilize the 2 pairs of allele specific pcr primer rice Molecular Detection seasonal febrile diseases resistant genes
Pi-bExistence:
Right with the Pib primer
Pib-F：GAACAATGCCCAAACTTGAGA；Pib-R：GGGTCCACATGTCAGTGAGC
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, contains if having this individual plant of 365 bp characteristic bands explanation
Pi-bGene;
Right with the Npib primer
Npib-F：TCGGTGCCTCGGTAGTCAGT；?Npib-R：GGGAAGCGGATCCTAGGTCT
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, does not contain if having this individual plant of 803 bp characteristic bands explanation
Pi-bGene;
At F
4Select in generation with the Pib primer to the characteristic band that amplifies 365bp, with the Npib primer individual plant that can not amplify 803bp purpose band is simultaneously
Pi-taThe individual plant of gene pure;
4) at F
4Select to contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant of gene pure is the rice varieties of the anti-Jiangsu Province japonica rice panicle blast of acquisition.
Perhaps with F
4Contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant that isozygotys of gene is planted into F respectively
5The sub-district, F
5The sub-district was carried out the panicle blast inoculation and is identified at the paddy rice booting initial stage, and by investigation standard survey result, the panicle blast standard of perfection is following, 0 grade after the paddy rice maturation: anosis, and immunity; 1 grade: 1/4 with morbidity of undershoot stalk or fringe neck spottiness, disease-resistant; 2 grades: branch stalk morbidity or main shaft middle part morbidity more than 1/4, or neck is ill, but little to yield effect, in anti-; 3 grades: main shaft middle part or neck morbidity, output there is appreciable impact, susceptible; 4 grades: the morbidity of fringe neck causes dead ears, high sense; It is the 0-1 level that selection shows as the panicle blast resistance to panicle blast) F
5The sub-district is the rice varieties of the anti-Jiangsu Province japonica rice panicle blast of acquisition.
5) with F
4Contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant that isozygotys of gene is planted into F respectively
5The sub-district, F
5It is stable to select the individual plant of 140 ~ 150 of plant height 90 ~ 100cm, every strain 8 ~ 10 fringes, every fringe to add generation in the sub-district, in F
6In generation, obtain the rice strain of economical character stable and consistent, anti-panicle blast.
Wherein rice strain Nanjing 14128, this strain plant height 98 ~ 102cm, every strain 8 ~ 10 fringes, average spike length 17 ~ 18cm, Erect Panicle, several 140 ~ 150 of every total grain panicle, ripening rate 94% ~ 98%, thousand kernel weight 26 ~ 27g.
Beneficial effect
A kind of breeding method of cultivating anti-Jiangsu Province japonica rice panicle blast new rice variety provided by the invention; Have the following advantages: the genetic marker that relates to assisted Selection in the inventive method is the PCR mark; Simple to operate, cost is low, not only can in breeding generation, identify rapidly and accurately and contain
Pi-ta, Pi-bHomozygotic rice plant owing to be the selection to gene itself, therefore can deduct the process that identify in the field simultaneously, simplifies the procedure of breeding, has reduced the breeding cost, has improved efficiency of selection, shortening the breeding cycle greatly.
Four, description of drawings
Fig. 1 Huaihe River rice 10/ southern round-grained rice 38F
4Colony's different individual plant kind disease-resistant gene
Pi-taMolecular Detection
(M:DL2000; 1: Huaihe River rice 10; 2: southern round-grained rice 38; 3-24:F
4The part individual plant of colony)
Fig. 2 Huaihe River rice 10/ southern round-grained rice 38F
4Colony's different individual plant kind disease-resistant gene
Pi-bMolecular Detection
(M:DL2000; 1: Huaihe River rice 10; 2: southern round-grained rice 38; 3-24:F
4The part individual plant of colony)
The anti-panicle blast high-yield variety of Fig. 3 " Nanjing 14128 " breeding line spectrogram
Five, embodiment
Select the parentHuaihe River rice No. 10 (original name Huaihe River 9836), Jiangsu Province's high yield japonica rice kind, Jiangsu Xuhuai Zone Huaiyin Agricultural Sciences Institute breeds, and Jiangsu Province's authorization in 2007 is also named.
Pi-taGenotype does on the site
Pi-taPi-ta,
Pi-bGenotype does on the site
Pi-bpi-b, the 155 days time of infertility of this kind, 96 ~ 100 centimetres of plant heights, 11 ~ 12 of the effective fringes of every strain, several 100 ~ 110 of the real grain of every fringe, ripening rate 90 ~ 92%, thousand kernel weight 25 ~ 26 grams.
South round-grained rice 38 (original name Nanjing 72031), Jiangsu Province's high yield japonica rice kind, Cereal Crops Research Inst., Jiangsu Agricultural Science Academy breeds, and calendar year 2001 Jiangsu Province's authorization is also named.
Pi-bGenotype does on the site
Pi-bPi-b,
Pi-taGenotype does on the site
Pi-tapi-ta, this kind leaf look delicate and pretty, and the later stage annesl is good, ripe lover.Plant height 90 ~ 95,11 ~ 12 of the effective fringes of every strain, several 120 ~ 125 of the real grain of every fringe, ripening rate 90 ~ 95%, thousand kernel weight 26 ~ 27 grams.
More than two kinds be public material, Jiangsu Province agricultural germ plasm resource storehouse in mid-term can provide free.Concrete list of references is: (Yuan Shengtang etc., seed selection and the application of Huaihe River rice No. 10 (Huaihe River 9836), Chinese rice, 2007,5,34-35; Zhong Weigong etc., the seed selection and the application of intermediate keng rice new varieties south round-grained rice 38, the Jiangsu agricultural science, 2001,5,35-37).
Breed breedingSelected for use in 2005
Pi-taGenotype does on the site
Pi-taPi-ta, high yield japonica rice kind Huaihe River, the Jiangsu rice of middle sense panicle blast be for No. 10 maternal ♀ with
Pi-bGenotype does on the site
Pi-bPi-bHigh yield and high quality sense panicle blast japonica rice variety south round-grained rice 38, be male parent ♂ hybridization combo, 2006 in Nanjing plantation F
1, plantation in 2007 mixes receives F
2For seed, plantation F in 2008
3, ripe back is mixed and is received seed.2009 at Nanjing plantation F
4, select the purpose individual plant according to the result of molecular mark, plantation F
5Individual plant is continued to select according to the result and the showing of economical character of molecular mark in the sub-district in the individual cells.Middle menu strain kind in 2010 becomes sub-district (F
6), filter out performance stable and consistent, high anti-panicle blast, the comprehensive good japonica rice new lines Nanjing 14128 of yielding ability.
Molecular marker assisted selectionF
4In generation, begin to carry out rice blast resistance gene
Pi-ta,
Pi-bMolecular marker assisted selection, seedling stage each individual plant DNA extraction adopt the SDS method (with reference to Dellaporta S L,
Et al.,
Plant Mol Biol Rep, 1983,1 (1): 19-21), slightly change.Concrete steps are: the water intaking rice seedling phase blade, in the mortar of-20 ℃ of precoolings with the liquid nitrogen grinding and the 1.5 ml centrifuge tubes of packing into; Add 600 ul extracts (20% SDS, 1M Tris-HCl, 0.5M EDTA, 5M NaCl, 65 ℃ of preheatings), shake up, 65 ℃ of temperature are bathed 30 min, middle vibration 3 ~ 4 times; Add 1/4 volume 5M KAC, shake up postposition 30 min on ice; Add chloroform-isoamyl alcohol (24:1) 300 ~ 400 ul, fully vibration on shaking table, 120 rpm, 30 min; Centrifugal 15 minutes of 8000 ~ 10000 rpm, the liquid level layering, lower floor's color is darker, and the little band yellow green in upper strata is got supernatant (about 400 ul) to another centrifuge tube; Add equal-volume chloroform-isoamyl alcohol (24:1), fully vibration on the shaking table, 80 ~ 90 rpm, 30 min; Centrifugal 15 minutes of 8000 rpm shift supernatant (about 400 ul) to new centrifuge tube; The absolute ethyl alcohol that adds 2 times of volume-20 ℃ precoolings shakes up up to there being floccule to produce centrifugal 6 min of 12000 rpm gently; Abandon absolute ethyl alcohol, add 4 ℃ of 70% ethanol, place 10 min, abandon supernatant, air-dry 1 h on the superclean bench; Add 100 ~ 200 ulTE ,-20 ℃ of preservations.
Rice blast resistance gene
Pi-taMolecular Detection, utilize 2 pairs of allele specific pcr primers:
Pita-F：AGCAGGTTATAAGCTAGGCC
Pita-R：CTACCAACAAGTTCATCAAA
Npita-F：AGCAGGTTATAAGCTAGCTAT
Npita-R；CTACCAACAAGTTCATCAAA
The genomic DNA of amplifying rice kind; The reaction system of 20 μ L comprises: template DNA (about 15ng/ μ L) 2 μ L, primer (4pmol/ μ L) 2 μ L, 10 * buffer solution (25 mmol/ L), 2 μ L; MgCl2 (25 mmol/ L) 1.2 μ L; DNTP (2.5 mmol/ L) 0. 4 μ L, Taq enzyme (5 U/ μ L) 0. 2 μ L, sterilization distilled water 12.2 μ L.On Eppendorf PCR appearance, increase, reaction condition is: (1) 94 ℃ of preparatory sex change 5 minutes; (2) 94 ℃, 1 minute; 58 ℃, 1 minute; 72 ℃, 1.5 minutes; Totally 35 circulations. (3) 72 ℃ were extended 10 minutes again.Product is carried out separation electrophoresis on the agarose 1%, ethidium bromide staining is observed on ultraviolet gel imaging appearance then and taken a picture, select Pita to amplify the characteristic band of 1042bp, and Npita can not amplify 1042bp purpose band simultaneously
Pi-taThe individual plant of gene pure.
Rice blast resistance gene
Pi-bMolecular Detection, utilize 2 pairs of allele specific pcr primers:
Pib-F：GAACAATGCCCAAACTTGAGA
Pib-R：GGGTCCACATGTCAGTGAGC
Npib-F：TCGGTGCCTCGGTAGTCAGT
Npib-R；GGGAAGCGGATCCTAGGTCT
The genomic DNA of amplifying rice kind; The reaction system of 20 μ L comprises: template DNA (about 15ng/ μ L) 2 μ L, primer (4pmol/ μ L) 2 μ L, 10 * buffer solution (25 mmol/ L), 2 μ L; MgCl2 (25 mmol/ L) 1.2 μ L; DNTP (2.5 mmol/ L) 0. 4 μ L, Taq enzyme (5 U/ μ L) 0. 2 μ L, sterilization distilled water 12.2 μ L.On Eppendorf PCR appearance, increase, reaction condition is: (1) 94 ℃ of preparatory sex change 5 minutes; (2) 94 ℃, 1 minute; 58 ℃, 1 minute; 72 ℃, 1.5 minutes; Totally 35 circulations. (3) 72 ℃ were extended 10 minutes again.Product is carried out separation electrophoresis on the agarose 1%, ethidium bromide staining is observed on ultraviolet gel imaging appearance then and taken a picture, select Pib to amplify the characteristic band of 365bp, and Npib can not amplify 803bp purpose band simultaneously
Pi-taThe individual plant of gene pure.
With F
4Contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant of gene pure is planted into F respectively
5The sub-district, at the paddy rice booting initial stage, with reference to sieve Chu equality (sieve Chu equality, rice blast inoculation technique and provinces and regions, Jiangsu in 2009 test product kind resistance are identified, Jiangsu Province's agricultural science, 2009,6:178-179) carry out the panicle blast inoculation and identify.After the paddy rice maturation, press investigation standard survey result.The panicle blast standard of perfection is following, 0 grade: anosis, and immunity; 1 grade: 1/4 with morbidity of undershoot stalk or fringe neck spottiness, disease-resistant; 2 grades: branch stalk morbidity or main shaft middle part morbidity more than 1/4, or neck is ill, but little to yield effect, in anti-; 3 grades: main shaft middle part or neck morbidity, output there is appreciable impact, susceptible; 4 grades: the morbidity of fringe neck causes dead ears, high sense.
Selection shows as disease-resistant (the panicle blast resistance is the 0-1 level) to panicle blast, contains simultaneously
Pi-ta,
Pi-bThe F of gene pure body
5The sub-district.
At above-mentioned F
5It is stable to select the individual plant of 140 ~ 150 of plant height 90 ~ 100cm, every strain 8 ~ 10 fringes, every fringe to add generation in the sub-district, in F
6In generation, obtain the rice strain of a collection of economical character stable and consistent, anti-panicle blast.Wherein rice strain Nanjing 14128, this strain plant height 98 ~ 102cm, every strain 8 ~ 10 fringes, average spike length 17 ~ 18cm, Erect Panicle, several 140 ~ 150 of every total grain panicle, ripening rate 94% ~ 98%, thousand kernel weight 26 ~ 27g.
SEQUENCE?LISTING
< 110>Jiangsu Province Agriculture Science Institute
< 120>a kind of breeding method of cultivating anti-Jiangsu Province panicle blast rice varieties
<130> 0
<160> 8
<170> PatentIn?version?3.1
<210> 1
<211> 20
<212> DNA
< 213>manual work
<220>
<221> Pita-F
<222> (1)..(20)
<223>
<400> 1
agcaggttat?aagctaggcc 20
<210> 2
<211> 20
<212> DNA
< 213>manual work
<220>
<221> Pita-R
<222> (1)..(20)
<223>
<400> 2
ctaccaacaa?gttcatcaaa 20
<210> 3
<211> 21
<212> DNA
< 213>manual work
<220>
<221> Npita-F
<222> (1)..(21)
<223>
<400> 3
agcaggttat?aagctagcta?t 21
<210> 4
<211> 20
<212> DNA
< 213>manual work
<220>
<221> Npita-R
<222> (1)..(20)
<223>
<400> 4
ctaccaacaa?gttcatcaaa 20
<210> 5
<211> 21
<212> DNA
< 213>manual work
<220>
<221> Pib-F
<222> (1)..(21)
<223>
<400> 5
gaacaatgcc?caaacttgag?a 21
<210> 6
<211> 20
<212> DNA
< 213>manual work
<220>
<221> Pib-R
<222> (1)..(20)
<223>
<400> 6
gggtccacat?gtcagtgagc 20
<210> 7
<211> 20
<212> DNA
< 213>manual work
<220>
<221> Npib-F
<222> (1)..(20)
<223>
<400> 7
tcggtgcctc?ggtagtcagt 20
<210> 8
<211> 20
<212> DNA
< 213>manual work
<220>
<221> Npib-R
<222> (1)..(20)
<223>
<400> 8
gggaagcgga?tcctaggtct 20
Claims (4)
1. breeding method of cultivating anti-Jiangsu Province japonica rice panicle blast new rice variety is characterized in that:
1) select for use middle sense panicle blast, genotype to do
Pi-taPi-tapi-bpi-bHigh-yield rice kind Huaihe River, Jiangsu rice 10 do maternally, with sense panicle blast, genotype do
Pi-tapi-taPi-bPi-bSouthern round-grained rice 38 make male parent preparing hybrid combo;
2) plantation F
1, F
2, F
3, receipts are mixed in ripe back all individual plant;
3) F
4In generation, begin to carry out the rice blast disease-resistant gene
Pi-ta, Pi-bMolecular marker assisted selection, seedling stage, the SDS method was adopted in the extraction of each individual plant DNA:
(1) utilize 2 pairs of allele specific pcr primer molecule to detect rice blast resistance gene
Pi-taExistence:
Right with the Pita primer
Pita-F：AGCAGGTTATAAGCTAGGCC；Pita-R：CTACCAACAAGTTCATCAAA
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, contains if having this individual plant of 1042 bp characteristic bands explanation
Pi-taGene;
Right with the Npita primer
Npita-F：AGCAGGTTATAAGCTAGCTAT；Npita-R：CTACCAACAAGTTCATCAAA
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, does not contain if having this individual plant of 1042 bp characteristic bands explanation
Pi-taGene;
At F
4To amplifying the characteristic band of 1042bp, the Npita primer is the individual plant that can not amplify 1042bp purpose band simultaneously with the Pita primer in selection in generation
Pi-taThe individual plant of gene pure;
(2) utilize the 2 pairs of allele specific pcr primer rice Molecular Detection seasonal febrile diseases resistant genes
Pi-bExistence:
Right with the Pib primer
Pib-F：GAACAATGCCCAAACTTGAGA；Pib-R：GGGTCCACATGTCAGTGAGC
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, contains if having this individual plant of 365 bp characteristic bands explanation
Pi-bGene;
Right with the Npib primer
Npib-F：TCGGTGCCTCGGTAGTCAGT
Npib-R：GGGAAGCGGATCCTAGGTCT
The genomic DNA of amplifying rice individual plant is also observed behind ethidium bromide staining, does not contain if having this individual plant of 803 bp characteristic bands explanation
Pi-bGene;
At F
4Select in generation with the Pib primer to the characteristic band that amplifies 365bp, with the Npib primer individual plant that can not amplify 803bp purpose band is simultaneously
Pi-taThe individual plant of gene pure;
4) at F
4Select to contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant of gene pure is the rice varieties of the anti-Jiangsu Province japonica rice panicle blast of acquisition.
2. breeding method according to claim 1 is characterized in that:
At F
4Select to contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant of gene pure is planted into F respectively
5The sub-district, F
5At the paddy rice booting initial stage, carry out the panicle blast inoculation and identify in the sub-district, by investigation standard survey result, the panicle blast standard of perfection is following, 0 grade after the paddy rice maturation: anosis, and immunity; 1 grade: 1/4 with morbidity of undershoot stalk or fringe neck spottiness, disease-resistant; 2 grades: branch stalk morbidity or main shaft middle part morbidity more than 1/4, or neck is ill, but little to yield effect, in anti-; 3 grades: main shaft middle part or neck morbidity, output there is appreciable impact, susceptible; 4 grades: the morbidity of fringe neck causes dead ears, high sense; It is the 0-1 level that selection shows as the panicle blast resistance to panicle blast) F
5The sub-district is the rice varieties of the anti-Jiangsu Province japonica rice panicle blast of acquisition.
3. breeding method according to claim 1 and 2 is characterized in that:
At F
4Select to contain simultaneously in generation
Pi-taGene pure with
Pi-bThe individual plant of gene pure is planted into F respectively
5The sub-district, F
5It is stable to select the individual plant of 140 ~ 150 of plant height 90 ~ 100cm, every strain 8 ~ 10 fringes, every fringe to add generation in the sub-district, in F
6In generation, obtain the rice strain of economical character stable and consistent, anti-panicle blast.
4. breeding method according to claim 3 is characterized in that:
Said rice strain Nanjing 14128, this strain plant height 98 ~ 102cm, every strain 8 ~ 10 fringes, average spike length 17 ~ 18cm, Erect Panicle, several 140 ~ 150 of every total grain panicle, ripening rate 94% ~ 98%, thousand kernel weight 26 ~ 27g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100357787A CN102577925A (en) | 2012-02-17 | 2012-02-17 | Breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100357787A CN102577925A (en) | 2012-02-17 | 2012-02-17 | Breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102577925A true CN102577925A (en) | 2012-07-18 |
Family
ID=46467615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100357787A Pending CN102577925A (en) | 2012-02-17 | 2012-02-17 | Breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102577925A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937890A (en) * | 2014-04-15 | 2014-07-23 | 江苏省农业科学院 | Multi-PCR method for identifying rice blast resistance genes Pi-ta and Pi-b |
| CN104542250A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Multi-gene high rice blast resistance material breeding method adopting marker assisted selection |
| CN104789654A (en) * | 2015-03-23 | 2015-07-22 | 华南农业大学 | Molecular marker for rice blast resistance gene Pita and application thereof |
| CN104969855A (en) * | 2015-07-30 | 2015-10-14 | 江苏里下河地区农业科学研究所 | Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance |
| CN105613255A (en) * | 2015-12-25 | 2016-06-01 | 江苏省农业科学院 | Pyramiding breeding method for rice blast resisting rice variety with excellent taste |
| CN105907884A (en) * | 2016-07-04 | 2016-08-31 | 湖北省农业科学院粮食作物研究所 | Rice blast resistant gene Pita specific molecular marker of rice and application |
| CN106167827A (en) * | 2016-08-11 | 2016-11-30 | 江苏焦点农业科技有限公司 | Resistance gene of rice blast Pita, Pib molecule labelling method |
| CN107027621A (en) * | 2017-05-04 | 2017-08-11 | 江苏省农业科学院 | A kind of anti-panicle blast, high-quality, the breeding method of high yield japonica rice |
| CN108018371A (en) * | 2017-12-21 | 2018-05-11 | 辽宁省盐碱地利用研究所 | Identify molecular labeling, identification method and the application of Rice Resistance To Rice Blast character |
| CN113016603A (en) * | 2021-03-09 | 2021-06-25 | 广西壮族自治区农业科学院 | Molecular breeding method of primary high-quality long-grain broad-spectrum rice blast-resistant normal recovery line |
| CN114391440A (en) * | 2022-01-21 | 2022-04-26 | 黑龙江省农业科学院耕作栽培研究所 | High-quality rice planting method |
| CN116949205A (en) * | 2022-11-02 | 2023-10-27 | 江苏省农业科学院 | Rice blast multiple disease-resistant gene combination pita+pitm+Pi5+Piz-t and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485283A (en) * | 2008-12-29 | 2009-07-22 | 重庆市农业科学院生物技术研究中心 | Method for improving selection efficiency in hybrid rice breeding |
-
2012
- 2012-02-17 CN CN2012100357787A patent/CN102577925A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485283A (en) * | 2008-12-29 | 2009-07-22 | 重庆市农业科学院生物技术研究中心 | Method for improving selection efficiency in hybrid rice breeding |
Non-Patent Citations (3)
| Title |
|---|
| 王军等: "Pi-ta、Pi-b基因在江苏粳稻穗颈瘟抗性育种中的价值分析", 《2011年中国作物学会学术年会论文摘要集》 * |
| 王军等: "利用分子标记辅助选择聚合水稻抗病基因Pi-ta、Pi-b和Stv-bi", 《作物学报》 * |
| 董巍等: "利用分子标记辅助选择改良培矮64S的稻瘟病抗性", 《分子植物育种》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937890B (en) * | 2014-04-15 | 2015-12-02 | 江苏省农业科学院 | A kind of multiple PCR method identifying rice blast resistance gene Pi-ta and Pi-b |
| CN103937890A (en) * | 2014-04-15 | 2014-07-23 | 江苏省农业科学院 | Multi-PCR method for identifying rice blast resistance genes Pi-ta and Pi-b |
| CN104542250A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Multi-gene high rice blast resistance material breeding method adopting marker assisted selection |
| CN104789654A (en) * | 2015-03-23 | 2015-07-22 | 华南农业大学 | Molecular marker for rice blast resistance gene Pita and application thereof |
| CN104969855A (en) * | 2015-07-30 | 2015-10-14 | 江苏里下河地区农业科学研究所 | Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance |
| CN105613255A (en) * | 2015-12-25 | 2016-06-01 | 江苏省农业科学院 | Pyramiding breeding method for rice blast resisting rice variety with excellent taste |
| CN105907884B (en) * | 2016-07-04 | 2019-06-21 | 湖北省农业科学院粮食作物研究所 | Rice blast resistant gene Pita specific molecular marker primer and application |
| CN105907884A (en) * | 2016-07-04 | 2016-08-31 | 湖北省农业科学院粮食作物研究所 | Rice blast resistant gene Pita specific molecular marker of rice and application |
| CN106167827A (en) * | 2016-08-11 | 2016-11-30 | 江苏焦点农业科技有限公司 | Resistance gene of rice blast Pita, Pib molecule labelling method |
| CN107027621A (en) * | 2017-05-04 | 2017-08-11 | 江苏省农业科学院 | A kind of anti-panicle blast, high-quality, the breeding method of high yield japonica rice |
| CN108018371A (en) * | 2017-12-21 | 2018-05-11 | 辽宁省盐碱地利用研究所 | Identify molecular labeling, identification method and the application of Rice Resistance To Rice Blast character |
| CN113016603A (en) * | 2021-03-09 | 2021-06-25 | 广西壮族自治区农业科学院 | Molecular breeding method of primary high-quality long-grain broad-spectrum rice blast-resistant normal recovery line |
| CN113016603B (en) * | 2021-03-09 | 2022-03-11 | 广西壮族自治区农业科学院 | Molecular breeding method of primary high-quality long-grain broad-spectrum rice blast-resistant normal recovery line |
| CN114391440A (en) * | 2022-01-21 | 2022-04-26 | 黑龙江省农业科学院耕作栽培研究所 | High-quality rice planting method |
| CN116949205A (en) * | 2022-11-02 | 2023-10-27 | 江苏省农业科学院 | Rice blast multiple disease-resistant gene combination pita+pitm+Pi5+Piz-t and application thereof |
| CN116949205B (en) * | 2022-11-02 | 2024-05-10 | 江苏省农业科学院 | Rice blast multiple disease-resistant gene combination Pita+ Pikm +Pi5+Piz-t and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102577925A (en) | Breeding method for breeding rice variety resistant to rice panicle blast in Jiangsu province | |
| Liu et al. | Confirming QTLs and finding additional loci responsible for resistance to rice sheath blight disease | |
| Kjøller et al. | Molecular diversity of glomalean (arbuscular mycorrhizal) fungi determined as distinct Glomus specific DNA sequences from roots of field grown peas fungi | |
| Rai et al. | Dry root rot of chickpea: a disease favored by drought | |
| CN101899506A (en) | Detection primer for No.1 and No.4 physiological strains of fusarium oxysporum f. sp cubense and rapid detection method | |
| CN104894228A (en) | Molecular marker and primer linked with Chinese cabbage clubroot disease resistant CRb gene and clubroot disease resistant plant selecting method | |
| Faghihi et al. | Witches'-broom disease of lime affects seed germination and seedling growth but is not seed transmissible | |
| Reis et al. | Outbreak of Fusarium oxysporum f. sp. lycopersici race 3 in commercial fresh-market tomato fields in Rio de Janeiro State, Brazil | |
| CN101338338B (en) | Molecule marker of red peel gene of weedy rice | |
| CN106755359A (en) | A kind of screening or the method and its dedicated kit of assisting sifting difference plant height wheat | |
| CN103937890B (en) | A kind of multiple PCR method identifying rice blast resistance gene Pi-ta and Pi-b | |
| CN104293906A (en) | SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof | |
| CN104278028B (en) | It is positioned at haynaldia villosa 6VS DNA and penetrates into wheat anti-powdery mildew NIL sequence and application | |
| CN110512021A (en) | One molecular labeling and its application with wheat base rot disease Resistance QTL close linkage | |
| CN104087575B (en) | The molecular labeling of the anti-fall gene prl4 of the strong stalk of paddy rice and application | |
| CN109234447A (en) | A kind of method for identifying No. 4 microspecies soybean resources of anti-soybean cyst nematode Heterodera glycines and dedicated SSR primer | |
| CN100392103C (en) | Molecular detection method for Fusarium circinatum | |
| CN104531886A (en) | D1 type rice cytoplasmic male sterile line and identification method of hybrid combination thereof | |
| Hönig et al. | Survey of Paxillus involutus (Batsch) Fr. inoculum and fruitbodies in a nursery by IGS-RFLPs and IGS sequences | |
| Youssef et al. | First report of Rhizoctonia solani AG 2-3 on chickpea in Tunisia | |
| CN110257545B (en) | Molecular marker for identifying hybrid paper mulberry and application thereof | |
| Ram et al. | Identification of donors for sheath blight resistance in wild species of rice | |
| CN102605074B (en) | Molecular marker method for identifying BT type cytoplasmmale sterility restoring geneRfla of japonica rice | |
| Lore et al. | Sources of resistance to sheath blight disease of rice caused by Rhizoctonia solani Kühn | |
| CN101328495B (en) | Method for detecting brome Tilletia foetida on broome by using PCR primer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120718 |