CN102575242B - 具有经修饰的前原区域的蛋白酶 - Google Patents

具有经修饰的前原区域的蛋白酶 Download PDF

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Publication number
CN102575242B
CN102575242B CN201080043790.1A CN201080043790A CN102575242B CN 102575242 B CN102575242 B CN 102575242B CN 201080043790 A CN201080043790 A CN 201080043790A CN 102575242 B CN102575242 B CN 102575242B
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Prior art keywords
protease
bacillus
host cell
seq
modified
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Expired - Fee Related
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CN201080043790.1A
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English (en)
Chinese (zh)
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CN102575242A (zh
Inventor
A·比萨奇科
B·F·施密特
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Danisco US Inc
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Danisco US Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201080043790.1A 2009-07-31 2010-04-15 具有经修饰的前原区域的蛋白酶 Expired - Fee Related CN102575242B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23024709P 2009-07-31 2009-07-31
US61/230,247 2009-07-31
PCT/US2010/031283 WO2011014278A1 (en) 2009-07-31 2010-04-15 Proteases with modified pre-pro regions

Publications (2)

Publication Number Publication Date
CN102575242A CN102575242A (zh) 2012-07-11
CN102575242B true CN102575242B (zh) 2015-03-25

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Family Applications (1)

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CN201080043790.1A Expired - Fee Related CN102575242B (zh) 2009-07-31 2010-04-15 具有经修饰的前原区域的蛋白酶

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Country Link
US (1) US20110171718A1 (enExample)
EP (1) EP2459714A1 (enExample)
JP (1) JP5852568B2 (enExample)
CN (1) CN102575242B (enExample)
AR (1) AR076311A1 (enExample)
BR (1) BR112012002163A2 (enExample)
CA (1) CA2769420A1 (enExample)
IN (1) IN2012DN00312A (enExample)
WO (1) WO2011014278A1 (enExample)

Families Citing this family (15)

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JP6378089B2 (ja) 2011-12-09 2018-08-22 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター
GB201212932D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
GB201212934D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
WO2016046234A2 (en) * 2014-09-22 2016-03-31 Nexttobe Ab Recombinant phe-free proteins for use in the treatment of phenylketonuria
US20180030456A1 (en) * 2015-02-19 2018-02-01 Danisco Us Inc. Enhanced protein expression
CA2989667A1 (en) * 2015-06-17 2016-12-22 Danisco Us Inc. Proteases with modified propeptide regions
FI3419991T3 (fi) 2016-03-04 2023-01-31 Modifioidut ribosomaaliset promoottorit proteiinien tuottamiseksi mikro-organismeissa
DE102016204815A1 (de) * 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Proteasen mit verbesserte Enzymstabilität in Waschmittel
DE102016204814A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen
DE102016208463A1 (de) * 2016-05-18 2017-11-23 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen
JP7609549B2 (ja) * 2016-05-31 2025-01-07 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
WO2021230358A1 (ja) * 2020-05-15 2021-11-18 Jnc株式会社 抗ウイルス剤
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1487080A (zh) * 2002-06-26 2004-04-07 花王株式会社 碱性蛋白酶

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US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US5431382A (en) * 1994-01-19 1995-07-11 Design Technology Corporation Fabric panel feed system
JP4210548B2 (ja) * 2002-06-26 2009-01-21 花王株式会社 アルカリプロテアーゼ
CN101597601B (zh) * 2002-06-26 2013-06-05 诺维信公司 具有改变的免疫原性的枯草杆菌酶和枯草杆菌酶变体
US20080020440A1 (en) * 2002-08-27 2008-01-24 Daniel Tillett Method of sequestering and/or purifying a polypeptide
US7807174B2 (en) * 2002-11-22 2010-10-05 Nexbio, Inc. Class of therapeutic protein based molecules
AU2003297317A1 (en) * 2002-12-13 2004-07-09 Case Western Reserve University Defensin-inducing peptides from fusobacterium
US7490416B2 (en) * 2004-01-26 2009-02-17 Townsend Herbert E Shoe with cushioning and speed enhancement midsole components and method for construction thereof
KR20090129425A (ko) * 2007-03-12 2009-12-16 다니스코 유에스 인크. 개질된 프로테아제
WO2008153925A2 (en) * 2007-06-06 2008-12-18 Danisco Us, Inc., Genencor Division Methods for improving multiple protein properties
CA2759695C (en) * 2009-04-24 2018-05-01 Danisco Us Inc. Proteases with modified pro regions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1487080A (zh) * 2002-06-26 2004-04-07 花王株式会社 碱性蛋白酶
US20040072321A1 (en) * 2002-06-26 2004-04-15 Kao Corporation Alkaline protease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
《Characterization and stability of proteases from Penicillium》;GERMANO ET AL;《ENZYME MICROB. TECHNOL》;20031231;第32卷;246-251 *

Also Published As

Publication number Publication date
US20110171718A1 (en) 2011-07-14
JP5852568B2 (ja) 2016-02-03
AR076311A1 (es) 2011-06-01
WO2011014278A1 (en) 2011-02-03
JP2013500714A (ja) 2013-01-10
EP2459714A1 (en) 2012-06-06
BR112012002163A2 (pt) 2015-11-03
CN102575242A (zh) 2012-07-11
IN2012DN00312A (enExample) 2015-05-08
CA2769420A1 (en) 2011-02-03

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Granted publication date: 20150325

Termination date: 20170415