CN102575242B - 具有经修饰的前原区域的蛋白酶 - Google Patents

具有经修饰的前原区域的蛋白酶 Download PDF

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Publication number
CN102575242B
CN102575242B CN201080043790.1A CN201080043790A CN102575242B CN 102575242 B CN102575242 B CN 102575242B CN 201080043790 A CN201080043790 A CN 201080043790A CN 102575242 B CN102575242 B CN 102575242B
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protease
bacillus
host cell
seq
modified
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Expired - Fee Related
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CN201080043790.1A
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English (en)
Chinese (zh)
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CN102575242A (zh
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A·比萨奇科
B·F·施密特
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Danisco USA Inc
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Danisco USA Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201080043790.1A 2009-07-31 2010-04-15 具有经修饰的前原区域的蛋白酶 Expired - Fee Related CN102575242B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23024709P 2009-07-31 2009-07-31
US61/230,247 2009-07-31
PCT/US2010/031283 WO2011014278A1 (en) 2009-07-31 2010-04-15 Proteases with modified pre-pro regions

Publications (2)

Publication Number Publication Date
CN102575242A CN102575242A (zh) 2012-07-11
CN102575242B true CN102575242B (zh) 2015-03-25

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CN201080043790.1A Expired - Fee Related CN102575242B (zh) 2009-07-31 2010-04-15 具有经修饰的前原区域的蛋白酶

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Country Link
US (1) US20110171718A1 (enExample)
EP (1) EP2459714A1 (enExample)
JP (1) JP5852568B2 (enExample)
CN (1) CN102575242B (enExample)
AR (1) AR076311A1 (enExample)
BR (1) BR112012002163A2 (enExample)
CA (1) CA2769420A1 (enExample)
IN (1) IN2012DN00312A (enExample)
WO (1) WO2011014278A1 (enExample)

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CN104053780A (zh) 2011-12-09 2014-09-17 丹尼斯科美国公司 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子
GB201212934D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
GB201212932D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
LT3197472T (lt) * 2014-09-22 2021-11-10 Tanea Medical Ab Rekombinantinių baltymų be fenilalanino naudojimas gydant fenilketonuriją
KR20170116159A (ko) 2015-02-19 2017-10-18 다니스코 유에스 인크. 향상된 단백질 발현
MX2017016499A (es) 2015-06-17 2018-03-12 Danisco Us Inc Proteasas con regiones de propeptido modificadas.
EP4095152A3 (en) 2016-03-04 2022-12-28 Danisco US Inc. Engineered ribosomal promoters for protein production in microorganisms
DE102016204815A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Proteasen mit verbesserte Enzymstabilität in Waschmittel
DE102016204814A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen
DE102016208463A1 (de) * 2016-05-18 2017-11-23 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen
US11661567B2 (en) 2016-05-31 2023-05-30 Danisco Us Inc. Protease variants and uses thereof
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
EP4151230A4 (en) * 2020-05-15 2023-11-08 JNC Corporation ANTIVIRAL AGENT
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1487080A (zh) * 2002-06-26 2004-04-07 花王株式会社 碱性蛋白酶

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US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US5431382A (en) * 1994-01-19 1995-07-11 Design Technology Corporation Fabric panel feed system
JP4210548B2 (ja) * 2002-06-26 2009-01-21 花王株式会社 アルカリプロテアーゼ
JP2005531307A (ja) * 2002-06-26 2005-10-20 ノボザイムス アクティーゼルスカブ 変化した免疫原性を有するスブチラーゼ及びスブチラーゼ変異体
US20080020440A1 (en) * 2002-08-27 2008-01-24 Daniel Tillett Method of sequestering and/or purifying a polypeptide
US7807174B2 (en) * 2002-11-22 2010-10-05 Nexbio, Inc. Class of therapeutic protein based molecules
WO2004055041A2 (en) * 2002-12-13 2004-07-01 Case Western Reserve University Defensin-inducing peptides from fusobacterium
US7490416B2 (en) * 2004-01-26 2009-02-17 Townsend Herbert E Shoe with cushioning and speed enhancement midsole components and method for construction thereof
CA2680563A1 (en) * 2007-03-12 2008-09-18 Danisco Us Inc. Modified proteases
CA2690023C (en) * 2007-06-06 2018-07-17 Danisco Us Inc. Methods for improving multiple protein properties
AR076312A1 (es) * 2009-04-24 2011-06-01 Danisco Us Inc Proteasas con regiones pro modificadas

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1487080A (zh) * 2002-06-26 2004-04-07 花王株式会社 碱性蛋白酶
US20040072321A1 (en) * 2002-06-26 2004-04-15 Kao Corporation Alkaline protease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
《Characterization and stability of proteases from Penicillium》;GERMANO ET AL;《ENZYME MICROB. TECHNOL》;20031231;第32卷;246-251 *

Also Published As

Publication number Publication date
JP2013500714A (ja) 2013-01-10
IN2012DN00312A (enExample) 2015-05-08
AR076311A1 (es) 2011-06-01
EP2459714A1 (en) 2012-06-06
US20110171718A1 (en) 2011-07-14
CN102575242A (zh) 2012-07-11
CA2769420A1 (en) 2011-02-03
WO2011014278A1 (en) 2011-02-03
BR112012002163A2 (pt) 2015-11-03
JP5852568B2 (ja) 2016-02-03

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Granted publication date: 20150325

Termination date: 20170415