CN102565382A - Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples - Google Patents
Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples Download PDFInfo
- Publication number
- CN102565382A CN102565382A CN2012100026233A CN201210002623A CN102565382A CN 102565382 A CN102565382 A CN 102565382A CN 2012100026233 A CN2012100026233 A CN 2012100026233A CN 201210002623 A CN201210002623 A CN 201210002623A CN 102565382 A CN102565382 A CN 102565382A
- Authority
- CN
- China
- Prior art keywords
- biotin
- anaphylactogen
- latex beads
- specific ige
- avidin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to an immunochromatography method for detecting allergen-specific IgE antibodies in blood samples, which comprises the steps of sequentially sticking a sample pad, a conjugating pad, chromatography film covered by a detection line and a quality control line and a water sucking pad in a mutually stagger mode on a bottom board to form an immunochromatography test strip, adding dropwise the blood samples to the sample pad to detect, and conjugating allergen-biotin-avidin-latex microspheres on the conjugating pad. The method achieve qualitative determination on the allergen-specific IgE antibodies by utilizing the fact that the allergen-specific IgE antibodies in the blood samples can conjugate with the allergen- biotin-avidin-latex microspheres to form a compound and the formed compound can be captured at the position of the detection line to show color bars. A biotin-avidin system is applied to connection of the allergen and the latex microspheres, an allergen is enabled to be connected with a plurality of latex microspheres, thereby the detecting sensitivity of the specific IgE antibodies is improved, and detecting results are clear and are easily observed.
Description
Technical field
the present invention relates to a kind of immune chromatography method that detects allergenic specific IgE antibody in the blood sample.
Background technology
immunochromatography is the immunoassay mode that comes across a kind of uniqueness at the initial stage eighties; It is a solid phase with the fibre strip chromatographic material often; Make sample solution swimming on chromatography strip through capillary action; And make on determinand and the chromatographic material in the sample acceptor (like antibody or antigen) to determinand that the immune response of high special, high-affinity takes place simultaneously; Immune complex is by enrichment or be trapped in certain zone (detect band) of chromatographic material in the chromatography process, through enzyme reaction or directly the label (like collaurum) that can estimate of utilization obtain experimental phenomena (like colour developing) intuitively.Free label is then crossed and is detected band, reaches the purpose of separating automatically with binding label.
immunochromatography technique has been widely used in the clinical treatment detection at present.The test item of domestic and international existing supply of commodities and bibliographical information mainly comprises following aspect: forbidden drug detects (amphetamine, cocaine, hemp, morphine, heroin etc.), the infectious disease cause of disease detects (microspironema pallidum, gonococcus, chlamydia trachomatis, helicobacter pylori etc.), hormone detection, parasite, tumor marker, cardiovascular disease detection mark etc.The common trace particle of immunochromatography technique has collaurum, latex, and electroselenium, gelatin etc., wherein the most successful label of utilization is a collaurum.
since under the normal condition SERUM IgE antibody detects to go out IgE only in the ng/ml level with the gel diffusion method of conventional determining IgG or IgM, thereby at present often the employing immunochromatographic method detect.Yet; The existing immunochromatographic method that carries out the SERUM IgE antibody test is directly connected to specific allergen and forms anaphylactogen-latex beads compound on the label latex beads; Be equivalent to one to one annexation, sensitivity is low, when being used to detect the specific IgE antibody of ng/ml level; Testing result is faint in color, and is not easy to observe.
(Biotin-Avidin-System BAS) is a kind of new bio reaction amplification system that grows up the end of the seventies to
biotin-avidin system.Along with the appearance of various biotin derivatives, BAS is widely used in each field of medical science very soon.In recent years big quantity research confirms that biotin-avidin system almost can combine with the various labels of present research success.The strong bonded of biotin-avidin and labelled reagent high affinity and multistage enlarge-effect make the immune labeled and relevant tracer analysis of BAS sensitive more.It has become the new technology that is widely used in micro-antigen, antibody qualitative and quantitative analysis and position observation research at present.
Summary of the invention
technical matters to be solved by this invention is the deficiency that overcomes prior art, and the immune chromatography method of allergenic specific IgE antibody in a kind of improved detection blood sample is provided.
For solving above technical matters, the present invention takes following technical scheme:
a kind of immune chromatography method that detects allergenic specific IgE antibody in the blood sample; Its at first with sample pad, pad, the chromatographic film that is coated with detection line and nature controlling line, adsorptive pads is interlaced successively sticks on the base plate; Constitute immuno-chromatographic test paper strip; In sample pad, dripping blood sample then detects; It is characterized in that: be combined with anaphylactogen-biotin-avidin-latex beads on the said pad; Said method utilizes the allergenic specific IgE antibody in the blood sample can combine to form bond with anaphylactogen-biotin-avidin-latex beads, and formed bond can be hunted down at the detection line place and presents colour band and realize the qualitative detection to allergenic specific IgE antibody.
are according to further embodiment of the present invention: described anaphylactogen-biotin-avidin-latex beads prepares through following method: at first anaphylactogen and biotin are connected to form anaphylactogen-biotin; The affinity element is connected to form affinity element-latex beads with latex beads; And then formed anaphylactogen-biotin is connected with formed affinity element-latex beads, promptly get.
According to a concrete aspect of the present invention, the concrete preparation process of anaphylactogen-biotin-avidin-latex beads is following:
1. anaphylactogen is dissolved in the phosphate buffer (PBS), biotin is dissolved in the deionized water, the WS with biotin drops in the phosphate buffer of anaphylactogen then, at room temperature stirs, and obtains containing the solution of anaphylactogen-biotin;
2. the affinity cellulose solution is dissolved in the MES damping fluid (MES),, takes out microballoon in centrifuge tube latex beads storage liquid concuss, centrifugal; Remove supernatant, add the MES damping fluid, concuss adds the MES damping fluid and the freshly prepared carbodiimide solution (EDC) that are dissolved with the affinity element; Concuss makes to mix, and hatches 20～40 minutes in the room temperature darkroom, adds Tween-20; Centrifugal, remove supernatant, add lauryl sodium sulfate aqueous solution (SDS) dissolving microballoon; Concuss, centrifugal, remove supernatant; Add phosphate buffer dissolving microballoon, concuss must contain the solution of affinity element-latex beads;
In the solution that contains affinity element-latex beads that 2. the solution adding step that contains anaphylactogen-biotin that 1.
3. make step makes; Stir gently until microballoon and all suspend; At room temperature continue then to stir 10～15 hours, promptly get the solution that contains anaphylactogen-biotin-avidin-latex beads.
are attached on the pad anaphylactogen-biotin-avidin-latex beads according to the present invention through spraying the said solution that contains anaphylactogen-biotin-avidin-latex beads.
preferably, are coated with anti erythrocyte antibody on sample pad.Detection line on the chromatographic film forms through encapsulating the mouse-anti human IgE monoclonal antibody.Nature controlling line forms through the polyclonal antibody that encapsulates the sheep anti human IgE.Chromatographic film is preferably NC Nitroncellulose film (NC film).
preferably, described latex beads is a polystyrene microsphere.
the invention still further relates to the application of biotin-avidin amplification system in immunochromatographic method.
Because the enforcement of above technical scheme, the present invention compared with prior art has following advantage:
the present invention is applied to connect anaphylactogen and latex beads with biotin-avidin system; Can be implemented in and connect a plurality of latex beadses on the anaphylactogen; Thereby the sensitivity of detection specificity IgE antibody is improved, and testing result is obvious, observes easily.
Embodiment
are done further detailed explanation below in conjunction with concrete embodiment to the present invention.
Embodiment 1
Present embodiment provides a kind of immunochromatographic method of SERUM IgE antibody, comprises the steps:
(1), the preparation anaphylactogen-biotin-avidin-latex beads, detailed process is following:
1. anaphylactogen connect biotin: anaphylactogen is dissolved among the PBS (pH 8.0) of 100mM, obtains the anaphylactogen solution of 1mg/ml.Biotin is dissolved in the deionized water, makes the biotin solution of 2mg/ml, this biotin solution slowly is added drop-wise in the anaphylactogen solution, the while agitating solution, and make container place 25 ℃ of water-baths.Dropwise, airtight container continues to stir 60 ± 5 minutes in 25 ℃ of water-baths, must contain the solution of anaphylactogen-biotin.
are the plain latex beads that connects of affinity 2.: the affinity element is dissolved in 100mM MES (pH 4.5) solution of 1ml, obtains the affinity cellulose solution of 1mg/ml.With microballoon storage liquid concuss 20s, take out 5.0 * 10
6
Microballoon in the Eppendorf centrifuge tube, with 8000g-10, the centrifugal 1-2min of the rotating speed of 000g removes the 100M MES (pH 4.5) that adds 0.5ml behind the supernatant; Concuss 20s adds 1ml affinity cellulose solution, adds the EDC of the freshly prepared 10mg/ml of 25 μ l, concuss; It is mixed, and 30min is hatched in the room temperature darkroom, adds 1.0ml 0.02% Tween-20, with 8000g-10; The centrifugal 1-2min of the rotating speed of 000g removes supernatant, obtains the microballoon of coupling, and the microballoon of coupling is dissolved in 1.0ml 0.1%SDS; Concuss, with 8000g-10, the centrifugal 1-2min of the rotating speed of 000g removes supernatant; Make microballoon be dissolved in the PBS (pH 8.0) of 100mM, concuss 20s, counting are placed on 4 ℃ of refrigerators preservations.
are being connected of anaphylactogen-biotin and affinity element-latex beads 3.: with 1ml anaphylactogen-biotin solution, add and be placed with in the container of isopyknic affinity element-latex beads; Stir gently up to all suspensions of microballoon with glass bar; Seal of vessel is placed on the mixer, and room temperature continues agitating solution 10-15 hour.
(2), the anaphylactogen-biotin-avidin for preparing-latex beads is sprayed on the pad.
(3), carry out encapsulating of chromatographic film: chromatographic film is the NC film, on chromatographic film, encapsulates the mouse-anti human IgE monoclonal antibody and forms detection line (T line); And the polyclonal antibody formation nature controlling line (C line) that encapsulates the sheep anti human IgE, the distance between T line and the C line is 5-10mm.
(4), on sample pad, encapsulate anti erythrocyte antibody.
(5), the assembling of immuno-chromatographic test paper strip: with sample pad, the pad that has combined anaphylactogen-biotin-avidin-latex beads, chromatographic film, adsorptive pads (filter paper) is interlaced successively sticks on the base plate; Cut into length 100-120mm; The test strips of width 8-12mm; The thickness of test strips is 0.1-1mm
The detection of
(6), sample: on the sample pad of test strips, drip 30 μ l positive serums (standard items that contain IgE), wait for 15min, judged result.Positive findings a: red stripes respectively occurs at the detection zone at detection line (T line) place and the Quality Control district at nature controlling line (C line) place; Negative findings: a purplish red colour band only occurs in the Quality Control district.If detection zone does not have colour band and purplish red colour band does not appear in the Quality Control district, show that incorrect operation or reagent are invalid, need detect test again.
Comparative Examples 1
this Comparative Examples provides a kind of immunochromatographic method of SERUM IgE antibody; Basically with embodiment 1; Different is that what the pad of the immuno-chromatographic test paper strip that it adopts combined is not anaphylactogen-biotin-avidin-latex beads, but has combined anaphylactogen-latex beads.The preparation process of this anaphylactogen-latex beads is following:
are dissolved in anaphylactogen among the 100mM MES (pH 4.5), get the anaphylactogen solution that concentration reaches 1mg/ml.With microballoon storage liquid concuss 20s, take out 5.0 * 10
6
Microballoon in the Eppendorf centrifuge tube, with 8000g-10, the centrifugal 1-2min of the rotating speed of 000g; Remove supernatant, add 50ul 100mM MES (pH 4.5), concuss 20s; Add 2ul anaphylactogen solution; Add the EDC of the freshly prepared 10mg/ml of 2.5ul, concuss mixes it.30min is hatched in the room temperature darkroom, adds 1.0ml 0.02% Tween-20, and with 8000g-10, the centrifugal 1-2min of the rotating speed of 000g removes supernatant, gets the microballoon of coupling.The microballoon of coupling is dissolved among the 1.0ml 0.1%SDS, concuss, with 8000g-10, the centrifugal 1-2min of the rotating speed of 000g removes supernatant, makes its PBS that is dissolved in 100mM (pH 8.0), concuss 20s.
The testing result of
contrast the above embodiments 1 and Comparative Examples 1 can know that the testing result color of Comparative Examples 1 is very dim, is not easy to observe, and shows that this method sensitivity is low; And the testing result of embodiment 1 is obvious, observes easily, shows highly sensitive.
The foregoing description only is explanation technical conceive of the present invention and characteristics; Its purpose is to let the personage who is familiar with this technology can understand content of the present invention and enforcement according to this; Can not limit protection scope of the present invention with this; All equivalences that spirit is done according to the present invention change or modify, and all should be encompassed within protection scope of the present invention
Claims (10)
1. immune chromatography method that detects allergenic specific IgE antibody in the blood sample; Its at first with sample pad, pad, the chromatographic film that is coated with detection line and nature controlling line, adsorptive pads is interlaced successively sticks on the base plate; Constitute immuno-chromatographic test paper strip; In sample pad, dripping blood sample then detects; It is characterized in that: be combined with anaphylactogen-biotin-avidin-latex beads on the said pad; Said method utilizes the allergenic specific IgE antibody in the blood sample can combine to form bond with anaphylactogen-biotin-avidin-latex beads, and formed bond can be hunted down at the detection line place and presents colour band and realize the qualitative detection to allergenic specific IgE antibody.
2. the immune chromatography method of allergenic specific IgE antibody in the detection blood sample according to claim 1; It is characterized in that: described anaphylactogen-biotin-avidin-latex beads prepares through following method: at first anaphylactogen and biotin are connected to form anaphylactogen-biotin; The affinity element is connected to form affinity element-latex beads with latex beads; And then formed anaphylactogen-biotin is connected with formed affinity element-latex beads, promptly get described anaphylactogen-biotin-avidin-latex beads.
3. the immune chromatography method of allergenic specific IgE antibody in the detection blood sample according to claim 2 is characterized in that: the concrete preparation process of described anaphylactogen-biotin-avidin-latex beads is following:
1. anaphylactogen is dissolved in the phosphate buffer, biotin is dissolved in the deionized water, the WS with biotin drops in the phosphate buffer of anaphylactogen then, at room temperature stirs, and obtains containing the solution of anaphylactogen-biotin;
2. the affinity cellulose solution is dissolved in the MES damping fluid,, takes out microballoon in centrifuge tube latex beads storage liquid concuss, centrifugal, remove supernatant; Add the MES damping fluid, concuss adds the MES damping fluid and the freshly prepared carbodiimide solution that are dissolved with the affinity element, concuss; Make to mix, hatched 20～40 minutes, add Tween-20 in the room temperature darkroom, centrifugal; Remove supernatant, add lauryl sodium sulfate aqueous solution dissolving microballoon, concuss, centrifugal; Remove supernatant, add phosphate buffer dissolving microballoon, concuss must contain the solution of affinity element-latex beads;
3. in the solution that contains affinity element-latex beads that 2. the solution adding step that contains anaphylactogen-biotin that 1. step is made makes; Stir gently until microballoon and all suspend; At room temperature continue then to stir 10～15 hours, promptly get the solution that contains anaphylactogen-biotin-avidin-latex beads.
4. the immune chromatography method of allergenic specific IgE antibody in the detection blood sample according to claim 4 is characterized in that: through spraying the said solution that contains anaphylactogen-biotin-avidin-latex beads anaphylactogen-biotin-avidin-latex beads is attached on the pad.
5. according to the immune chromatography method of allergenic specific IgE antibody in the described detection blood sample of each claim in the claim 1 to 4, it is characterized in that: be coated with anti erythrocyte antibody on the described sample pad.
6. according to the immune chromatography method of allergenic specific IgE antibody in the described detection blood sample of each claim in the claim 1 to 4, it is characterized in that: described detection line forms through encapsulating the mouse-anti human IgE monoclonal antibody.
7. according to the immune chromatography method of allergenic specific IgE antibody in the described detection blood sample of each claim in the claim 1 to 4, it is characterized in that: described nature controlling line forms through the polyclonal antibody that encapsulates the sheep anti human IgE.
8. according to the immune chromatography method of allergenic specific IgE antibody in the described detection blood sample of each claim in the claim 1 to 4, it is characterized in that: described chromatographic film is the NC Nitroncellulose film.
9. according to the immune chromatography method of allergenic specific IgE antibody in the described detection blood sample of each claim in the claim 1 to 4, it is characterized in that: described latex beads is a polystyrene microsphere.
10. the application of biotin-avidin amplification system in immunochromatographic method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100026233A CN102565382B (en) | 2012-01-06 | 2012-01-06 | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100026233A CN102565382B (en) | 2012-01-06 | 2012-01-06 | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102565382A true CN102565382A (en) | 2012-07-11 |
| CN102565382B CN102565382B (en) | 2013-12-04 |
Family
ID=46411340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100026233A Active CN102565382B (en) | 2012-01-06 | 2012-01-06 | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102565382B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044326A (en) * | 2015-09-11 | 2015-11-11 | 苏州浩欧博生物医药有限公司 | Kit and method for achieving highly-sensitive multiterm joint inspection of allergen-specific IgE antibodies |
| CN111308064A (en) * | 2020-02-27 | 2020-06-19 | 四川新健康成生物股份有限公司 | Method for improving sensitivity of immunochromatography marker and application of immunochromatography marker in interleukin 6 detection |
| CN113495139A (en) * | 2021-07-07 | 2021-10-12 | 上海清芮科技有限公司 | Method for detecting atopic constitution of pet |
| CN113552338A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Allergen specificity IgE antibody detection kit and preparation method thereof |
| AU2020376757B2 (en) * | 2019-10-27 | 2022-08-25 | Ke Zhang | A reverse LFIA for IgE |
| CN116400070A (en) * | 2023-06-07 | 2023-07-07 | 济南德亨医学科技有限公司 | Preparation method of reagent for quantitatively detecting allergen-specific IgE antibody with high flux |
| EP4045909A4 (en) * | 2019-10-17 | 2024-02-21 | Monash University | Methods for detecting immune response |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415759A (en) * | 2002-10-14 | 2003-05-07 | 上海华冠生物芯片有限公司 | Marking probe of nano microparticle and affinity element and its preparation method as well as application |
| CN1508545A (en) * | 2002-12-17 | 2004-06-30 | 湖州瑞泽生物技术有限公司 | Immune colloidal gold reagent for detecting clenbuterol hydrochloride and preparation method thereof |
| CN1743845A (en) * | 2005-09-01 | 2006-03-08 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | A kind of detection method of protein chip |
| WO2006122450A1 (en) * | 2005-05-20 | 2006-11-23 | Calypte Biomedical Corporation | Oral fluid rapid immunochromatography test |
| CN101017169A (en) * | 2006-07-26 | 2007-08-15 | 艾博生物医药(杭州)有限公司 | Analysis equipment of biological sample |
| CN101078724A (en) * | 2007-06-08 | 2007-11-28 | 艾博生物医药(杭州)有限公司 | Detection device and method for detecting analyte |
| CN101614737A (en) * | 2009-06-02 | 2009-12-30 | 青岛康地恩药业有限公司 | A kind of colloidal gold strip and its production and application |
-
2012
- 2012-01-06 CN CN2012100026233A patent/CN102565382B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415759A (en) * | 2002-10-14 | 2003-05-07 | 上海华冠生物芯片有限公司 | Marking probe of nano microparticle and affinity element and its preparation method as well as application |
| CN1508545A (en) * | 2002-12-17 | 2004-06-30 | 湖州瑞泽生物技术有限公司 | Immune colloidal gold reagent for detecting clenbuterol hydrochloride and preparation method thereof |
| WO2006122450A1 (en) * | 2005-05-20 | 2006-11-23 | Calypte Biomedical Corporation | Oral fluid rapid immunochromatography test |
| CN101495865A (en) * | 2005-05-20 | 2009-07-29 | 克利普特生物医药公司 | Rapid immunity chromatography detection of mouth cavity fluid |
| CN1743845A (en) * | 2005-09-01 | 2006-03-08 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | A kind of detection method of protein chip |
| CN101017169A (en) * | 2006-07-26 | 2007-08-15 | 艾博生物医药(杭州)有限公司 | Analysis equipment of biological sample |
| CN101078724A (en) * | 2007-06-08 | 2007-11-28 | 艾博生物医药(杭州)有限公司 | Detection device and method for detecting analyte |
| CN101614737A (en) * | 2009-06-02 | 2009-12-30 | 青岛康地恩药业有限公司 | A kind of colloidal gold strip and its production and application |
Non-Patent Citations (3)
| Title |
|---|
| BERIT GRANUM ET AL: "Fine particles of widely different composition have an adjuvant effect on the production of allergen-specific antibodies", 《TOXICOLOGY LETTERS》 * |
| BERIT GRANUM ET AL: "Fine particles of widely different composition have an adjuvant effect on the production of allergen-specific antibodies", 《TOXICOLOGY LETTERS》, vol. 118, no. 3, 3 January 2001 (2001-01-03), pages 171 - 181 * |
| 吉坤美等: "胶体金免疫层析法检测食品中花生过敏原蛋白成分", 《食品研究与开发》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044326A (en) * | 2015-09-11 | 2015-11-11 | 苏州浩欧博生物医药有限公司 | Kit and method for achieving highly-sensitive multiterm joint inspection of allergen-specific IgE antibodies |
| EP4045909A4 (en) * | 2019-10-17 | 2024-02-21 | Monash University | Methods for detecting immune response |
| AU2020376757B2 (en) * | 2019-10-27 | 2022-08-25 | Ke Zhang | A reverse LFIA for IgE |
| CN111308064A (en) * | 2020-02-27 | 2020-06-19 | 四川新健康成生物股份有限公司 | Method for improving sensitivity of immunochromatography marker and application of immunochromatography marker in interleukin 6 detection |
| CN111308064B (en) * | 2020-02-27 | 2024-04-09 | 四川新健康成生物股份有限公司 | Method for improving sensitivity of immunochromatography marker and application of method in detection of interleukin 6 |
| CN113552338A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Allergen specificity IgE antibody detection kit and preparation method thereof |
| CN113495139A (en) * | 2021-07-07 | 2021-10-12 | 上海清芮科技有限公司 | Method for detecting atopic constitution of pet |
| CN116400070A (en) * | 2023-06-07 | 2023-07-07 | 济南德亨医学科技有限公司 | Preparation method of reagent for quantitatively detecting allergen-specific IgE antibody with high flux |
| CN116400070B (en) * | 2023-06-07 | 2023-08-18 | 济南德亨医学科技有限公司 | Preparation method of reagent for quantitatively detecting allergen-specific IgE antibody with high flux |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102565382B (en) | 2013-12-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102565382B (en) | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples | |
| CN101165491B (en) | Method for detecting double-chain DNA resistant antibody using gold magnetic particle as carrier | |
| CN105765388B (en) | Improved pregnancy tests apparatus and method | |
| US5518887A (en) | Immunoassays empolying generic anti-hapten antibodies and materials for use therein | |
| AU2008258509B2 (en) | Detection of antigens carried by erythrocytes and of anti-erythrocyte antibodies | |
| CN101441210B (en) | Nano magnetic particle chromatography test paper detection method | |
| EP1240517B1 (en) | Analyte assay device with preabsorbing zone | |
| EP2574926A1 (en) | Chromatographic kit and chromatography method | |
| US5270166A (en) | Immunoassays employing generic anti-hapten antibodies and materials for use therein | |
| CN105195243B (en) | The magnetic microparticle chemiluminescence micro-fluidic chip that a kind of myoglobins is quantitatively detected | |
| WO1984002193A1 (en) | Chromogenic support immunoassay | |
| CN101553729A (en) | Saturation assay | |
| CN101435819A (en) | Immunity-chromatography test utensil | |
| WO2015119386A1 (en) | High-sensitivity and lateral-flow immunochromatographic chip using enzyme-mimic inorganic nanoparticles and detection method using same | |
| CN105334325A (en) | Microfluidic immune chip analysis method based on biotin and streptavidine system | |
| CN109342718A (en) | A kind of magnetic microparticle chemiluminescence detection method | |
| CN106526166A (en) | Rapid detection of lean meat powder in pork | |
| CN203224494U (en) | Detection test strip for allergen-specific IgE (immunoglobulin E) antibodies | |
| EP0609265A4 (en) | Unit-of-use reagent composition for specific binding assays. | |
| CA2110920A1 (en) | Assay for the detection of specific ligands | |
| KR100411406B1 (en) | Inspection kit | |
| CN106370843A (en) | Quick measurement method of lean meat powder on the basis of gold magnetic immuno-chromatography | |
| CN104297489A (en) | Anti-PR3-ANC antibody detection test paper preparation method and purpose thereof | |
| CN217639110U (en) | High-sensitivity immunochromatography detection kit | |
| CN102539765A (en) | Triazolam detection kit and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Xinghu Street Industrial Park of Suzhou city in Jiangsu province 215123 building C6 No. 218 Patentee after: Jiangsu Hao Bo biomedical Limited by Share Ltd Address before: Xinghu Street Industrial Park of Suzhou city in Jiangsu province 215123 building C6 No. 218 Patentee before: Suzhou Haooubo Biopharmaceutical Co., Ltd. |