CN102539765A - Triazolam detection kit and preparation method thereof - Google Patents
Triazolam detection kit and preparation method thereof Download PDFInfo
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- CN102539765A CN102539765A CN2010106109048A CN201010610904A CN102539765A CN 102539765 A CN102539765 A CN 102539765A CN 2010106109048 A CN2010106109048 A CN 2010106109048A CN 201010610904 A CN201010610904 A CN 201010610904A CN 102539765 A CN102539765 A CN 102539765A
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Abstract
The invention discloses a detection kit for detecting triazolam. The kit comprises a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample suction pad (4) and a PVC (Polyvinyl Chloride) supporting plate (5), wherein the sample pad, the colloidal gold pad, the nitrocellulose membrane and the sample suction pad are sequentially and continuously adsorbed to the PVC supporting plate; the colloidal gold pad is a colloidal gold-labeled triazolam monoclonal antibody polyester membrane; and the nitrocellulose membrane is sequentially coated by triazolam-bovine serum albumin coupling antigen as a detection line (T line) and goat anti-mouse IgG polyclonal antibody as a quality control line (C line). The triazolam detection kit is prepared by using a colloidal gold immunochromatographic assay technology and is used for detecting triazolam which probably exists in the sample; and the kit has simple preparation method and has the characteristics of convenience in use, quick response, economy, practicability and the like.
Description
Technical field
The present invention relates to the determination techniques field of biology immunization method, particularly relate to a kind of a kind of triazolam detection kit of utilizing colloid gold immune layer fabrication techniques and preparation method thereof.
Background technology
Triazolam has another name called Halcion, Halcion, and the pale blue color chips is a kind of strong narcotics, can the people gone into a coma after oral and fall in a swoon, so be commonly called as magic potion, knockout drops, magic potion.Can follow alcohol type to take jointly, also in water soluble and the various beverage.Take effect rapidly, drug effect than common stable strong 45-100 doubly.Triazolam is one of current domestic the most frequently used benzodiazepine, often is used for anesthesia by the offender and plunders and anaesthetize homicide.It is reported that it is fast that triazolam absorbs metabolism in vivo, its main metabolites is the Alpha-hydroxy triazolam.Because triazolam dosage is few, triazolam in biological material and metabolite content thereof are all very low, therefore it are extracted the check difficulty.
The detection method of relevant triazolam has a lot; For example: methods such as high performance liquid chromatography, vapor-phase chromatography, GC-MS; Though the sensitivity that these methods detect is higher, testing result is accurate, and existence needs expensive instrument and equipment; Requirement to test material is high, needs restrictions such as purification processes.Therefore the detection method of research with advantage such as quick, portable has realistic meaning.The present invention has introduced a kind of detection kit with colloidal gold immunity chromatography fast detecting triazolam and preparation method thereof; This method has simply, fast; Need not any instrument and equipment, characteristics such as economical and practical are applicable to fast qualitative detects in the sample whether contain triazolam.
The colloidal gold immunochromatographimethod technology is a novel vitro diagnostic techniques that on monoclonal antibody technique, immunochromatography technique and collaurum developing technology basis, has grown up since 20th century the nineties.Can prepare colloid gold test paper based on the colloidal gold immunochromatographimethod technology; The principle of colloid gold test paper is to use colloidal gold-labeled method; With the collaurum is tracer, utilizes the antigen and antibody specific association reaction, utilize specific chromogenic reaction on test paper colour developing and judge the result of detection; Have simple to operate, quick, highly sensitive, the high specificity of detection, need not complicated characteristics such as instrument.
Summary of the invention
The object of the present invention is to provide triazolam kit of a kind of simple, quick, highly sensitive, high specificity and preparation method thereof, be used for detecting sample to be checked and whether contain triazolam.
A kind of detection kit that is used to detect triazolam comprises sample pad, collaurum pad, nitrocellulose filter, suction appearance pad and PVC back up pad, and tight adhesion has sample pad, collaurum pad, nitrocellulose filter and suction appearance pad successively on the PVC back up pad.Said sample pad is a spun glass; The triazolam monoclonal antibody polyester film that said collaurum pad is a colloid gold label; Be coated with detection line (T line) and nature controlling line (C line) on the said nitrocellulose filter successively, wherein encapsulated triazolam-bovine serum albumin coupled antigen on the detection line (T line), encapsulated the sheep anti-mouse igg polyclonal antibody on the nature controlling line (C line); Said suction appearance pad is thieving paper.
The present invention adopts nano-colloid technology for gold and antigen and antibody specific reaction; The principle of using immunity competition inhibitory reaction is prepared from; The triazolam monoclonal antibody of the triazolam-bovine serum albumin coupled antigen competition association colloid gold mark that encapsulates through detection line (T line) on the triazolam that contains in the sample to be checked and the nitrocellulose filter judges whether contain triazolam in the sample to be checked through the colour developing of T line.
The invention provides a kind of preparation method of triazolam detection kit, may further comprise the steps:
(1) preparation triazolam coupled antigen
With triazolam and bovine serum albumin coupling, synthetic triazolam-bovine serum albumin coupled antigen is as the triazolam coupled antigen.
(2) preparation triazolam monoclonal antibody
Adopting triazolam-bovine serum albumin coupled antigen is the immunogen immune BALB/C mice, through hybridoma technology, obtains secreting the hybridoma cell strain of anti-triazolam monoclonal antibody; Prepare antibody in a large number to induce the ascites method in the body, use Protein G post to carry out purifying, obtain the triazolam monoclonal antibody.
(3) preparation collaurum
Get 0.01% aqueous solution of chloraurate, heated and boiled.Add 1% citric acid, three sodium water solution 1ml as required rapidly, continue to boil about 5min, occur orange red.The size of the colloid gold particle of processing like this is 20-40nm.
(4) preparation collaurum pad
Get the colloidal gold solution that grain size is 20-40nm, use 0.1mol/L K
2CO
3The pH value of colloidal gold solution is transferred to 7.0-9.0, and room temperature was placed 30 minutes; In above-mentioned solution, add the triazolam monoclonal antibody, the concentration that makes the triazolam monoclonal antibody is 10-60 μ g/ml collaurum, and after mixing, room temperature was placed 30 minutes; It is 10-60 μ l/ml that bovine serum albumin (BSA) solution of adding 10% makes its concentration, mixes, and room temperature was placed 30 minutes; 12000 left the heart 30 minutes, carefully drew supernatant, discarded, and remaining deposition is with the collaurum redissolution liquid dissolving of initial collaurum volume; 12000 left the heart 30 minutes, carefully drew supernatant, discarded, and remaining deposition obtains triazolam monoclonal antibody-colloid gold label thing with the collaurum redissolution liquid dissolving of the initial collaurum volume of 30%-100%; The ratio of triazolam monoclonal antibody-colloid gold label thing in 1mL shop 40-70cm2 polyester film evenly is layered on the polyester film, puts drying room again, 38 ℃ of temperature, humidity was processed the collaurum pad less than under 30% the condition dry 24 ± 2 hours.
Above-mentioned collaurum redissolution liquid is the Tris that contains 0.01-0.1%, the PBS of the 0.02M pH7.0-9.0 of the sucrose of 1.0-3.0%, the BSA of 0.1-1.0%.
(4) encapsulate triazolam-bovine serum albumin coupled antigen, sheep anti-mouse igg polyclonal antibody
Set and draw film appearance coating parameters 1 μ L/cm; Use respectively microsyringe get concentration as triazolam-bovine serum albumin coupled antigen of 0.5-5.0mg/ml, get concentration and be 0.5-5.0mg/ml sheep anti-mouse igg polyclonal antibody, receive A, the B pipe joint of drawing the film appearance in order.The PVC plate that posts nitrocellulose filter is placed on the to-and-fro movement platform of drawing the film appearance, open and draw the film appearance, on nitrocellulose filter, apply triazolam-bovine serum albumin coupled antigen (T line), sheep anti-mouse igg polyclonal antibody (C line).Preserve line back in the baking oven of 38 ℃ of temperature dry 24 ± 2 hours, subsequent use.
(5) processing of sample pad
Spun glass is soaked 20-40min in the PBS of 0.01M pH 7.0-8.0, wherein contain 0.5-1.5%BSA in the PBS, 0.5-1.0%Tweeen-20,38 ℃ of oven dry in drying baker are preserved, and are subsequent use
(6) assembling kit
On the PVC back up pad, adhere to sample pad, collaurum pad, nitrocellulose filter and suction appearance pad in order successively, obtain the said test strips that is used to detect triazolam, test strips can be packed in the plastic clip, is assembled into test card.Wherein said sample pad is a spun glass, inhales the appearance pad and is thieving paper.
The detection method of kit according to the invention is: with the test sample balance to room temperature; Take out the triazolam pick-up unit, horizontal positioned; In sample pad, add 2-3 and drip sample, observe and write down the colour developing situation of C, T line in the time of 10-15 minute, judge testing result.
Kit of the present invention adopts colloidal gold immunochromatographimethod technical measurement triazolam, during detection, sample is added on the sample pad on the kit, can observe directly immunoreactive result, accomplishes sample detection.The present invention can be used for detecting the triazolam that possibly exist in the sample, have easy to use, simple to operate, be swift in response, characteristics such as economical and practical.
Description of drawings
Fig. 1 triazolam detection kit structural representation;
The reference numeral explanation:
1: sample pad;
2: collaurum pad (polyester film of colloid gold label triazolam monoclonal antibody)
3: nitrocellulose filter (T: the detection line that has encapsulated triazolam-bovine serum albumin coupled antigen; C: the nature controlling line that has encapsulated the sheep anti-mouse igg polyclonal antibody);
4: inhale the appearance pad;
The 5:PVC back up pad;
The testing result synoptic diagram of Fig. 2 kit of the present invention.
Be followed successively by line positive test symbol of C line from left to right; T, two line negative result of C; Invalid.
Embodiment:
Embodiment 1: the preparation of triazolam detection kit
1. prepare the triazolam coupled antigen
With triazolam and bovine serum albumin coupling, synthetic triazolam-bovine serum albumin coupled antigen is as the triazolam coupled antigen.
2. prepare the triazolam monoclonal antibody
Adopting triazolam-bovine serum albumin coupled antigen is the immunogen immune BALB/C mice, through hybridoma technology, obtains secreting the hybridoma cell strain of anti-triazolam monoclonal antibody; Prepare antibody in a large number to induce the ascites method in the body, use Protein G post to carry out purifying, obtain the triazolam monoclonal antibody.
3. preparation collaurum
Get 0.01% aqueous solution of chloraurate 100ml, heated and boiled.Add 1% citric acid, three sodium water solution 1ml as required rapidly, continue to boil about 5min, occur orange red.The size of the colloid gold particle of processing like this is 20-40nm.
4. prepare the collaurum pad
Getting grain size is the colloidal gold solution 5ml of 20-40nm, adds 0.15ml 0.1mol/L K
2CO
3The pH value of colloidal gold solution is transferred to 8.0, and room temperature was placed 30 minutes; In above-mentioned solution, adding 0.0375ml concentration is the triazolam monoclonal antibody of 4.0mg/ml, and after mixing, room temperature was placed 30 minutes; Add bovine serum albumin (BSA) solution of 0.25ml 10%, mix, room temperature was placed 30 minutes; 12000 left the heart 30 minutes, carefully drew supernatant, discarded, and remaining deposition is with the collaurum redissolution liquid dissolving of initial collaurum volume; 12000 left the heart 30 minutes, carefully drew supernatant, discarded, and remaining deposition obtains triazolam monoclonal antibody-colloid gold label thing with the collaurum redissolution liquid dissolving of 50% initial collaurum volume; Triazolam monoclonal antibody-colloid gold label thing is pressed 1mL shop 56cm
2The ratio of polyester film evenly is layered on the polyester film, puts drying room again, and 38 ℃ of temperature, humidity was processed the collaurum pad less than under 30% the condition dry 24 hours.
Above-mentioned collaurum redissolution liquid is to contain 0.01% Tris, the PBS of the 0.02M pH 8.0 of 2.0% sucrose, 0.5% BSA.
4. encapsulate triazolam-bovine serum albumin coupled antigen, sheep anti-mouse igg polyclonal antibody
Set to draw film appearance coating parameters 1 a μ L/cm, use respectively microsyringe get concentration as triazolam-bovine serum albumin coupled antigen of 2.0mg/ml, get concentration and be 1.2mg/ml sheep anti-mouse igg polyclonal antibody, receive A, the B pipe joint of drawing the film appearance in order.The PVC plate that posts nitrocellulose filter is placed on the to-and-fro movement platform of drawing the film appearance, open and draw the film appearance, on nitrocellulose filter, apply triazolam-bovine serum albumin coupled antigen (T line), sheep anti-mouse igg polyclonal antibody (C line).Preserve line back in the baking oven of 38 ℃ of temperature dry 24 ± 2 hours, subsequent use.
5. the processing of sample pad
Spun glass is soaked 30min in the PBS treating fluid of 50ml 0.01M pH 8.0, wherein contain 1.0%BSA in the PBS, 0.5%Tweeen-2,38 ℃ of oven dry in drying baker are preserved, and are subsequent use
6. assembling kit
On the PVC back up pad, adhere to sample pad, collaurum pad, nitrocellulose filter and suction appearance pad in order successively, obtain the said test strips that is used to detect triazolam, test strips can be packed in the plastic clip, is assembled into test card.Wherein said sample pad is a spun glass, inhales the appearance pad and is thieving paper.
Embodiment 2: the detection of triazolam detection kit
1. detection method:
Take out the triazolam detection kit, horizontal positioned; On sample pad, splash into 3 samples, observe and write down the colour developing situation of C, T line after 10 minutes, judge testing result.
2. the result judges
Positive: the T line does not develop the color, and only C line colour developing is judged to be positive findings;
Negative: T line, C line all develop the color, and are judged to be negative findings;
Invalid: the C line does not develop the color, and the rotten damage of maloperation or kit is described.
During test sample, sample fills up an end chromatography because of capillarity to inhaling appearance.If contain triazolam in the sample; They will and detection line (T line) on limited antibody combining site on the triazolam monoclonal antibody of the triazolam-bovine serum albumin coupled antigen competition association colloid gold mark that encapsulates; When the triazolam in the sample reaches finite concentration; Also saturated fully with the triazolam monoclonal antibody generation immune response of colloid gold label; This moment, colloidal gold composite did not have the triazolam-bovine serum albumin coupled antigen combination that encapsulates on vacant site and the detection line, and this moment, the T line did not develop the color this positive result.If do not contain triazolam in the sample; Mark the colloid gold particle of triazolam monoclonal antibody will be to the T line position in company with the sample chromatography; Immune association reaction takes place with the triazolam that encapsulates on the T line-bovine serum albumin coupled antigen; Colloid gold particle is piled up at the T line position and is made the T line demonstrate a macroscopic red stripes, this negative result.No matter whether contain triazolam in the sample; The colloid gold label thing all can combine with the sheep anti-mouse igg polyclonal antibody on being coated on nature controlling line (C line) and develop the color; C line colour developing is to judge whether enough samples are arranged, and whether the chromatography process normal standard, simultaneously also as the inner quality standard of reagent.
Embodiment 3: the applicating evaluating of triazolam detection kit
1. limit of identification
With phosphate buffer solution (PBS:0.01mol/L, pH 7.5) compound concentration be 0,400,500, the triazolam standard items of 600ng/mL; Each concentration is carried out 10 parallel testings respectively; Observe testing result in the time of 10 minutes; The testing result of the triazolam standard items of 0ng/mL, 400ng/mL is that T line, C line all develop the color negative result; The testing result of the triazolam standard items of 500ng/mL, 600ng/mL is that the T line does not develop the color, the colour developing of C line, is judged to be positive test symbol; The limit of identification of final definite triazolam detection kit is 500ng/ml.
2. negative with reference to the article coincidence rate
With phosphate buffer solution (PBS:0.01mol/L, pH 7.5) compound concentration is that the methamphetamine standard solution of 100ug/mL carries out 10 parallel testings, observes testing result in the time of 10 minutes, and T line, C line all present red stripes, and the result is negative.
With phosphate buffer solution (PBS:0.01mol/L, pH 7.5) compound concentration is that the methadone standard solution of 100ug/mL carries out 10 parallel testings, observes testing result in the time of 10 minutes, and T line, C line all present red stripes, and the result is negative.
3. positive with reference to the article coincidence rate
With phosphate buffer solution (PBS:0.01mol/L, pH 7.5) compound concentration is the triazolam standard items of 500ng/mL, 600ng/mL, 700ng/mL; Each concentration is carried out 10 parallel testings respectively; Observe testing result in the time of 10 minutes, the T line does not develop the color, the colour developing of C line, and the result is positive.
4. repeated
With phosphate buffer solution (PBS:0.01mol/L, pH 7.5) compound concentration is the triazolam standard items of 500ng/mL, carries out 10 parallel testings, observes testing result in the time of 10 minutes; The result is the T line and does not develop the color; The colour developing of C line, and colour developing degree homogeneous, positive result.
5. stable
Place after 20 days for 37 ℃, limit of identification, negative reference article coincidence rate, positive reference article coincidence rate, repeated each item index all meet above requirement, and this kit has good stable property.
Claims (6)
1. triazolam detection kit and preparation method thereof; It is characterized in that being made up of sample pad, collaurum pad, nitrocellulose filter, suction appearance pad and PVC back up pad, sample pad, collaurum pad, nitrocellulose filter and suction appearance pad tight adhesion are on the PVC back up pad; Said sample pad is a spun glass; The triazolam monoclonal antibody polyester film that said collaurum pad is a colloid gold label; Encapsulated triazolam-bovine serum albumin(BSA) coupled antigen on the said nitrocellulose filter successively as detection line (T line), the sheep anti-mouse igg polyclonal antibody is as nature controlling line (C line); Said suction appearance pad is thieving paper.
2. described triazolam detection kit of claim 1 and preparation method thereof is characterized in that described triazolam monoclonal antibody is obtained as the immunogen immune BALB/C mice by triazolam-bovine serum albumin coupled antigen.
3. described triazolam detection kit of claim 1 and preparation method thereof is characterized in that described collaurum is by gold chloride (HAuCl
4) under the effect of reductive agent citric acid trisodium, process size and be the colloid gold particle of 20-40nm.
4. described triazolam detection kit of claim 1 and preparation method thereof is characterized in that the preparation method of described collaurum pad is: get the colloidal gold solution that grain size is 20-40nm, use 0.1mol/L K
2CO
3The pH value of colloidal gold solution is transferred to 7.0-9.0, and room temperature was placed 30 minutes; In above-mentioned solution, add the triazolam monoclonal antibody, the concentration that makes the triazolam monoclonal antibody is 10-60 μ g/ml collaurum, and after mixing, room temperature was placed 30 minutes; It is 10-60 μ l/ml that bovine serum albumin (BSA) solution of adding 10% makes its concentration, mixes, and room temperature was placed 30 minutes; 12000 left the heart 30 minutes, carefully drew supernatant, discarded, and remaining deposition is with the collaurum redissolution liquid dissolving of initial collaurum volume; 12000 left the heart 30 minutes, carefully drew supernatant, discarded, and remaining deposition obtains triazolam monoclonal antibody-colloid gold label thing with the collaurum redissolution liquid dissolving of the initial collaurum volume of 30%-100%; Triazolam monoclonal antibody-colloid gold label thing is pressed 1mL shop 40-70cm
2The ratio of polyester film evenly is layered on the polyester film, puts drying room again, and 38 ℃ of temperature, humidity was processed the collaurum pad less than under 30% the condition dry 24 ± 2 hours.
5. a claim 1 and the described triazolam detection kit of claim 5 and preparation method thereof; It is characterized in that described collaurum redissolution liquid is the Tris that contains 0.01-0.1%, the PBS of the 0.02M pH7.0-9.0 of the sucrose of 1.0-3.0%, the BSA of 0.1-1.0%.
6. described triazolam detection kit of claim 1 and preparation method thereof; The method for coating that it is characterized in that two lines on the described nitrocellulose filter is: set and draw film appearance coating parameters 1 μ L/cm; Use respectively microsyringe get concentration as triazolam-bovine serum albumin coupled antigen of 0.5-5.0mg/ml, get concentration and be 0.5-5.0mg/ml sheep anti-mouse igg polyclonal antibody, receive A, the B pipe joint of drawing the film appearance in order.The PVC plate that posts nitrocellulose filter is placed on the to-and-fro movement platform of drawing the film appearance, open and draw the film appearance, on nitrocellulose filter, apply triazolam-bovine serum albumin coupled antigen (T line), sheep anti-mouse igg polyclonal antibody (C line).Preserve line back in the baking oven of 38 ℃ of temperature dry 24 ± 2 hours, subsequent use.
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| CN2010106109048A CN102539765A (en) | 2010-12-29 | 2010-12-29 | Triazolam detection kit and preparation method thereof |
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| CN2010106109048A CN102539765A (en) | 2010-12-29 | 2010-12-29 | Triazolam detection kit and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103880847A (en) * | 2014-03-14 | 2014-06-25 | 公安部物证鉴定中心 | Deuterated triazolam and preparation method thereof |
| CN107167593A (en) * | 2017-06-02 | 2017-09-15 | 亳州市新健康科技有限公司 | Illicit drugs inspection kit |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2357331Y (en) * | 1998-11-04 | 2000-01-05 | 北京市齐明经济技术发展公司 | Hard drug testing box |
| US6248598B1 (en) * | 1998-09-17 | 2001-06-19 | Stuart C. Bogema | Immunoassay that provides for both collection of saliva and assay of saliva for one or more analytes with visual readout |
| US20020137761A1 (en) * | 2001-03-23 | 2002-09-26 | Crain Stanley M. | Methods for increasing analgesic potency and attenuating adverse excitatory effects of bimodally -acting opioid agonists by inhibiting GM1-ganglioside |
| CN1488941A (en) * | 2002-10-09 | 2004-04-14 | 上海市刑事科学技术研究所 | Test paper for detecting morphine |
| CN2914091Y (en) * | 2006-02-22 | 2007-06-20 | 万华普曼生物工程有限公司 | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine |
| CN101576563A (en) * | 2009-06-19 | 2009-11-11 | 暨南大学 | Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof |
-
2010
- 2010-12-29 CN CN2010106109048A patent/CN102539765A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6248598B1 (en) * | 1998-09-17 | 2001-06-19 | Stuart C. Bogema | Immunoassay that provides for both collection of saliva and assay of saliva for one or more analytes with visual readout |
| CN2357331Y (en) * | 1998-11-04 | 2000-01-05 | 北京市齐明经济技术发展公司 | Hard drug testing box |
| US20020137761A1 (en) * | 2001-03-23 | 2002-09-26 | Crain Stanley M. | Methods for increasing analgesic potency and attenuating adverse excitatory effects of bimodally -acting opioid agonists by inhibiting GM1-ganglioside |
| CN1488941A (en) * | 2002-10-09 | 2004-04-14 | 上海市刑事科学技术研究所 | Test paper for detecting morphine |
| CN2914091Y (en) * | 2006-02-22 | 2007-06-20 | 万华普曼生物工程有限公司 | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine |
| CN101576563A (en) * | 2009-06-19 | 2009-11-11 | 暨南大学 | Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 宋娟 等: "半抗原的设计、修饰及人工抗原的制备", 《分析化学评述与进展》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103880847A (en) * | 2014-03-14 | 2014-06-25 | 公安部物证鉴定中心 | Deuterated triazolam and preparation method thereof |
| CN103880847B (en) * | 2014-03-14 | 2016-03-23 | 公安部物证鉴定中心 | Deuterated triazolam and preparation method thereof |
| CN107167593A (en) * | 2017-06-02 | 2017-09-15 | 亳州市新健康科技有限公司 | Illicit drugs inspection kit |
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Application publication date: 20120704 |