CN102559765A - Yeast culture and application thereof in promoting proliferation of intestinal probiotics. - Google Patents
Yeast culture and application thereof in promoting proliferation of intestinal probiotics. Download PDFInfo
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- CN102559765A CN102559765A CN2012100519620A CN201210051962A CN102559765A CN 102559765 A CN102559765 A CN 102559765A CN 2012100519620 A CN2012100519620 A CN 2012100519620A CN 201210051962 A CN201210051962 A CN 201210051962A CN 102559765 A CN102559765 A CN 102559765A
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Abstract
The invention discloses a yeast culture and application thereof in promoting proliferation of intestinal probiotics. The invention provides a method for preparing the yeast culture. The method comprises the following step of fermenting Saccharomyces cerevisiae with the collection number of CGMCC No. 2.1882 to obtain the yeast culture. The invention provides a process for preparing the yeast culture, by which the yeast culture with excellent performance is obtained. The yeast culture provided by the invention contains a variety of nutrients and can provide a nutrient substrate and nutritional active substances for growth of probiotics, thereby promoting the proliferation of the probiotics. The yeast culture and the application thereof in promoting the proliferation of the intestinal probiotics are of great value in the field of food or feed.
Description
Technical field
The present invention relates to a kind of yeast culture and the application in promoting beneficial bacteria of intestinal tract propagation thereof.
Background technology
Along with popularizing even abuse of feeding antibiotic, negative effects such as the resistance that it brought, drug residue become increasingly conspicuous.The restriction of feeding antibiotic is used and forbidden is the inexorable trend of animal husbandry development; And development and use alternative promote intestinal beneficial bacterium propagation, improve intestinal environment, improve animal body immunizing power, simultaneously not by the enteron aisle endoenzyme decomposition, the novel green fodder additives that do not produce resistance, noresidue be the inevitable choice of sustainable development of animal husbandry.
Yeast culture (yeast culture; YC) be meant by yeast under the specific process conditions through abundant formed little ecological goods in fermentation back, mainly comprise yeast cell meta-bolites, the substratum that after fermentation, makes a variation and the yeast cell of non-activity on a small quantity.As a kind of biologically active additives, nutritious, the complicated component of yeast culture contains VITAMINs, amino acid, digestive ferment, organic acid, polysaccharide and unidentified growth factor etc.According to relevant report, yeast culture can effectively improve the animal gastrointestinal tract flora, promotes probiotics breedings such as milk-acid bacteria, CELLULOLYTIC BACTERIUM.
The quality of yeast culture receives the influence of factors such as bacterial classification, fermentation manufacturing technique and detection means.Yeast like differing temps, humidity or acid or alkali environment especially substrate composition, can produce different tunnings, and this wherein comprises a large amount of UGFs under the different culture environment.Employed substratum and zymotechnique controlled variable have fundamental influence to the stability of composition, concentration and the bioavailability thereof of meta-bolites in its end product when producing yeast culture.Even if using under the situation of identical barms, different substratum are formed or different fermentative prodn CONTROL PROCESS can cause in the yeast culture product meta-bolites to be formed and evident difference appears in concentration.
Summary of the invention
The purpose of this invention is to provide a kind of yeast culture and the application in promoting beneficial bacteria of intestinal tract propagation thereof.
The invention provides a kind of method for preparing yeast culture, is CGMCC to be numbered 2.1882 yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ferment, and obtains yeast culture.
Said fermentation can comprise the steps:
(1) said yeast saccharomyces cerevisiae is seeded to seed culture medium, 18-40h (like 18-24h or 24-40h) is cultivated in 20-50 ℃ (as 20-28 ℃ or 28-50 ℃), 100-200rpm (like 100-150rpm or 150-200rpm) jolting, obtains seed liquor;
(2) seed liquor with step (1) is seeded to fermention medium, and 20-50 ℃ (as 20-30 ℃ or 30-50 ℃) leaves standstill and cultivate 30-60h (30-45h or 45-60h).
The preparation method of said fermention medium is following: get 20-200g carbon source, 5-300g nitrogenous source, 0.1-10g (like 0.1-1g or 1-10g) KH
2PO
4, 0.1-10g (like 0.1-1g or 1-10g) MgSO
47H
2O, 0.1-4g (like 0.1-1g or 1-4g) MnSO
4H
2O, 0.1-4g (like 0.1-1g or 1-4g) CaCl
2, 0.1-4g (like 0.1-1g or 1-4g) CuSO
45H
2O, 0.1-4g (like 0.1-1g or 1-4g) ZnSO
47H
2O and 0.1-4g (like 0.1-1g or 1-4g) FeSO
44H
2O, water (like zero(ppm) water) dissolve and are settled to 1L.
Said 20-200g carbon source specifically can be made up of the carbohydrate that 10-100g glucose (like 10-50g or 50-100g) and 10-100g (like 10-50g or 50-100g) contain sucrose.Said 20-200g carbon source specifically can be the carbohydrate that 20-200g (like 20-100g or 100-200g) glucose or 20-200g (like 20-100g or 100-200g) contain sucrose.The said carbohydrate that contains sucrose is specially sucrose or molasses.
Said 5-300g nitrogenous source specifically can be made up of 2-100g (like 2-50g or 50-100g) yeast extract paste, 2-100g (like 2-50g or 50-100g) peptone and 1-100g (like 1-50g or 50-100g) ammonium sulfate; Also can form by 2.5-150g (like 2.5-70g or 70-150g) yeast extract paste and 2.5-150g (like 2.5-70g or 70-150g) peptone; Also can form, also can form by 2.5-150g (like 2.5-70g or 70-150g) peptone and 2.5-150g (like 2.5-70g or 70-150g) ammonium sulfate by 2.5-150g (like 2.5-70g or 70-150g) yeast extract paste and 2.5-150g (like 2.5-70g or 70-150g) ammonium sulfate.Said 5-300g nitrogenous source specifically can be 5-300g yeast extract paste or 5-300g peptone or 5-300g ammonium sulfate.
The preparation method of said seed culture medium is specific as follows: get 10-40g (like 10-20g or 20-40g) glucose, 5-40g (like 5-10g or 10-40g) yeast powder, 10-40g (like 10-20g or 20-40g) peptone and 0.1-4g (like 0.1-2g or 2-4g) MnSO
4H
2O, water (like zero(ppm) water) dissolve and are settled to 1L.
The pH value of said seed culture medium specifically can be nature pH.
The inoculum size of said seed liquor is 1%-30% (like 1%-15% or 15%-30%).The volume ratio of special seed liquor of the inoculum size in this patent and fermention medium.
Said seed liquor is seeded to the initial time of said fermention medium, and the concentration of said yeast saccharomyces cerevisiae is 1 * 10
5-3 * 10
6Cfu/ml is (as 1 * 10
5-1.5 * 10
6Cfu/ml or 1.5 * 10
6-3 * 10
6Cfu/ml).
Said method also comprises the steps: the centrifugal 5min of total system 4000rpm that accomplishes fermentation is collected supernatant.
Said method also comprises said fermentation supernatant with the filtering step of biofilter (0.22 μ m).
More than the yeast culture for preparing of arbitrary said method all belong to protection scope of the present invention.
Said yeast culture can be used for promoting beneficial bacteria of intestinal tract propagation.Said beneficial bacteria of intestinal tract specifically can be animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus or lactobacillus delbruockii subspecies bulgaricus.
Said yeast culture can be used for preparing the preparation that promotes beneficial bacteria of intestinal tract propagation.Said beneficial bacteria of intestinal tract specifically can be animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus or lactobacillus delbruockii subspecies bulgaricus.
The present invention also protects a kind of method of cultivating beneficial bacteria of intestinal tract, is to add beneficial bacteria of intestinal tract (probiotic bacterium seed liquor) and said yeast culture at the substratum that is used for cultivating said beneficial bacteria of intestinal tract, obtains the initial incubation system, cultivates then.Said beneficial bacteria of intestinal tract specifically can be animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus or lactobacillus delbruockii subspecies bulgaricus.In the said initial incubation system, the add-on of said yeast culture can be 0.1%-20% volume ratio (like 0.1%-10% volume ratio or 10%-20% volume ratio).In the said initial incubation system, the add-on of said probiotics bacterial liquid can be 0.1%-20% volume ratio (like 0.1%-10% volume ratio or 10%-20% volume ratio).In the said initial incubation system, said yeast culture and said probiotics bacterial liquid specifically can be 1: 1 volume ratio.It is 10 that said probiotics bacterial liquid specifically can be concentration
7The bacterium liquid of cfu/ml.
Said animal bifidobacteria specifically can be CGMCC and is numbered 1.2268 bacterial strain.Said Lactobacterium acidophilum specifically can be CGMCC and is numbered 1.1878 bacterial strain.Said plant lactobacillus specifically can be CGMCC and is numbered 1.557 bacterial strain.Said thermophilus streptococcus specifically can be CGMCC and is numbered 1.2471 bacterial strain.Said lactobacillus delbruockii subspecies bulgaricus specifically can be CGMCC and is numbered 1.1480 bacterial strain.
The invention provides a kind of preparation technology of yeast culture, and obtained the yeast culture of excellent property.Contain various nutrients in the yeast culture provided by the invention, can nutrition substrate and nutrient active matter be provided, thereby promote beneficial bacteria of intestinal tract propagation for the beneficial bacteria of intestinal tract growth.The present invention has great value for field of food or field of fodder.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment like no specified otherwise, is ordinary method.Used test materials among the following embodiment like no specified otherwise, is to buy from routine biochemistry reagent shop and obtains.Quantitative test in following examples all is provided with repeated experiments three times, results averaged.All adopt HCl or NaOH to regulate the pH value of substratum among the embodiment.
Substratum used among the embodiment is following:
MRS substratum: available from Qingdao Hai Bo Bioisystech Co., Ltd, catalog number: HB0384-1.
PYG substratum: available from Qingdao Hai Bo Bioisystech Co., Ltd, catalog number: HB0398.
Glucose-yeast extract medium (pH 7.0): take by weighing 1.0g glucose, 5.0g peptone, 2.5g yeast extract paste, with dissolved in distilled water and be settled to 1L.
Bacterial strain used among the embodiment is following:
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae): CGMCC is numbered 2.1882.
Animal bifidobacteria (Bifidobacterium animalis is called for short BA): CGMCC is numbered 1.2268.
Lactobacterium acidophilum (Lactobacillus acidophilus is called for short LA): CGMCC is numbered 1.1878.
Plant lactobacillus (Lactobacillus plantarum is called for short LP): CGMCC is numbered 1.557.
Thermophilus streptococcus (Streptococcus thermophilus): CGMCC is numbered 1.2471.
Lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.bulgaricus): CGMCC is numbered 1.1480.
The preparation of embodiment 1, yeast culture and application
One, the preparation of substratum
Seed culture medium (natural pH): take by weighing 10g glucose, 5g yeast powder, 40g peptone and 4g MnSO
4H
2O is with dissolved in distilled water and be settled to 1L; 121 ℃, 0.1Mpa high-temperature sterilization 15min, subsequent use.
Fermention medium (pH4.0): take by weighing carbon source (10g glucose and 10g sucrose), nitrogenous source (2g yeast extract paste, 2g peptone and 1g ammonium sulfate), 10g KH
2PO
4, 10g MgSO
47H
2O, 4g MnSO
4H
2O, 4g CaCl
2, 4g CuSO
45H
2O, 4g ZnSO
47H
2O and 4g FeSO
44H
2O is with dissolved in distilled water and be settled to 1L; 121 ℃, 0.1Mpa high-temperature sterilization 15min, subsequent use.
Two, the preparation of yeast culture
1, the yeast saccharomyces cerevisiae after the activation is accessed in the seed culture medium, 28 ℃, 150rpm (shimmy amplitude 25mm) shaking culture 24h obtain seed liquor.
2, in the 250ml triangular flask, the 2ml seed liquor is seeded to 200ml fermention medium (being that inoculum size is 1%), the initial system that obtains fermenting, the concentration of yeast saccharomyces cerevisiae is 1 * 10 in the initial system of fermenting
5Cfu/ml; 20 ℃ of standing for fermentation 60h of the initial system of will fermenting, the termination system obtains fermenting.
3, the centrifugal 5min of termination system 4000rpm (centrifugal radius is 13.5cm) that will ferment collects supernatant, and supernatant is filtered with biofilter (0.22 μ m), obtains cell-free filtrate (checking aseptic growing through dull and stereotyped), claims aseptic yeast culture again.
Three, the application of yeast culture
Five kinds of beneficial bacteria of intestinal tract (animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus and lactobacillus delbruockii subspecies bulgaricus) are tested respectively as follows:
1, the beneficial bacteria of intestinal tract after the activation is resuspended with sterilized water, obtaining bacteria concentration is 10
7The bacteria suspension of cfu/ml.
2, packet transaction
Experimental group: (Lactobacterium acidophilum, plant lactobacillus and lactobacillus delbruockii subspecies bulgaricus all adopt the MRS substratum in centrifuge tube, to add aseptic culture medium; Animal bifidobacteria adopts the PYG substratum; Thermophilus streptococcus adopts glucose-yeast extract medium), the bacteria suspension that obtains of the aseptic yeast culture that obtains of step 2 and step 1, be initial system (liquid amount of centrifuge tube is 50%); The volumn concentration of aseptic yeast culture in initial system is 0.1%, and the volumn concentration of bacteria suspension in initial system is 0.1%.
Control group: sterilized water is replaced the aseptic yeast culture in the experimental group, other same experimental group.
Build the stopper of centrifuge tube in each group, cultivate 24h for 37 ℃.
3, cultivate the end back and adopt ultraviolet-uisible spectrophotometer to measure the OD600 (characterizing the biomass of beneficial bacteria of intestinal tract) in total system, the results are shown in Table 1.
The experimental group among table 1 embodiment 1 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.648 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.666 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.513 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.535 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.487 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 1.5 times, the OD600 of Lactobacterium acidophilum and has improved 1.8 times, the OD600 of plant lactobacillus and improved 1.3 times, the OD600 of lactobacillus delbruockii subspecies bulgaricus and improved 0.9 times, the OD600 of thermophilus streptococcus and improved 1.2 times.
The preparation of embodiment 2, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): take by weighing 40g glucose, 40g yeast powder, 10g peptone and 0.1gMnSO
4H
2O is with being settled to 1L behind the dissolved in distilled water; 121 ℃, 0.1Mpa high-temperature sterilization 15min, subsequent use.
Fermention medium (pH5.5): take by weighing carbon source (100g glucose and 100g sucrose), nitrogenous source (100g yeast extract paste, 100g peptone and 100g ammonium sulfate), 0.1g KH
2PO
4, 0.1g MgSO
47H
2O, 0.1g MnSO
4H
2O, 0.1g CaCl
2, 0.1g CuSO
45H
2O, 0.1g ZnSO
47H
2O and 0.1g FeSO
44H
2O is with dissolved in distilled water and be settled to 1L; 121 ℃, 0.1Mpa high-temperature sterilization 15min, subsequent use.
Two, the preparation of yeast culture
1, the yeast saccharomyces cerevisiae after the activation is accessed in the seed culture medium, 20 ℃, 100rpm (shimmy amplitude 25mm) shaking culture 18h obtain seed liquor.
2, in the 250ml triangular flask, the 60ml seed liquor is seeded to 200ml fermention medium (being that inoculum size is 30%), the initial system that obtains fermenting, the concentration of yeast saccharomyces cerevisiae is 3 * 10 in the initial system of fermenting
6Cfu/ml; 50 ℃ of standing for fermentation 30h of the initial system of will fermenting, the termination system obtains fermenting.
3, the centrifugal 5min of termination system 4000rpm (centrifugal radius 13.5cm) that will ferment collects supernatant, and supernatant is filtered with biofilter (0.22 μ m), obtains cell-free filtrate (checking aseptic growing through dull and stereotyped), claims aseptic yeast culture again.
Three, the application of yeast culture
Five kinds of beneficial bacteria of intestinal tract (animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus and lactobacillus delbruockii subspecies bulgaricus) are tested respectively as follows:
1, with 1 of the step 3 of embodiment 1.
2, packet transaction
Experimental group: (Lactobacterium acidophilum, plant lactobacillus and lactobacillus delbruockii subspecies bulgaricus all adopt the MRS substratum in centrifuge tube, to add aseptic culture medium; Animal bifidobacteria adopts the PYG substratum; Thermophilus streptococcus adopts glucose-yeast extract medium) in, the bacteria suspension that obtains of the aseptic yeast culture that obtains of step 2 and step 1, be initial system (liquid amount of centrifuge tube is 50%); The volumn concentration of aseptic yeast culture in initial system is 20%, and the volumn concentration of bacteria suspension in initial system is 20%.
Control group: fermention medium is replaced the aseptic yeast culture in the experimental group, other same experimental group.
Build the stopper of centrifuge tube in each group, cultivate 24h for 37 ℃.
3, cultivate the end back and adopt ultraviolet-uisible spectrophotometer to measure the OD600 (characterizing the biomass of beneficial bacteria of intestinal tract) in total system, the results are shown in Table 2.
The experimental group among table 2 embodiment 2 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.803 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.690 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.580 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.753 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.640 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 2.1 times, the OD600 of Lactobacterium acidophilum and has improved 1.9 times, the OD600 of plant lactobacillus and improved 1.6 times, the OD600 of lactobacillus delbruockii subspecies bulgaricus and improved 1.5 times, the OD600 of thermophilus streptococcus and improved 2.1 times.
The preparation of embodiment 3, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): take by weighing 20g glucose, 10g yeast powder, 20g peptone and 2g MnSO
4H
2O is with dissolved in distilled water and be settled to 1L; 121 ℃, 0.1Mpa high-temperature sterilization 15min, subsequent use.
Fermention medium (pH6.5) takes by weighing carbon source (50g glucose and 50g sucrose), nitrogenous source (50g yeast extract paste, 50g peptone and 50g ammonium sulfate), 1g KH
2PO
4, 1g MgSO
47H
2O, 1g MnSO
4H
2O, 1g CaCl
2, 1g CuSO
45H
2O, 1g ZnSO
47H
2O and 1g FeSO
44H
2O is with dissolved in distilled water and be settled to 1L; 121 ℃, 0.1Mpa high-temperature sterilization 15min, subsequent use.
Two, the preparation of yeast culture
1, the yeast saccharomyces cerevisiae after the activation is accessed in the seed culture medium, 50 ℃, 200rpm (shimmy amplitude 25mm) shaking culture 40h obtain seed liquor.
2, in the 250ml triangular flask, the 30ml seed liquor is seeded to 200ml fermention medium (being that inoculum size is 15% volume ratio), the initial system that obtains fermenting, the concentration of yeast saccharomyces cerevisiae is 1.5 * 10 in the initial system of fermenting
6Cfu/ml; 30 ℃ of standing for fermentation 45h of the initial system of will fermenting, the termination system obtains fermenting.
3, the centrifugal 5min of termination system 4000rpm (centrifugal radius 13.5cm) that will ferment collects supernatant, and supernatant is filtered with biofilter (0.22 μ m), obtains cell-free filtrate (checking aseptic growing through dull and stereotyped), claims aseptic yeast culture again.
Three, the application of yeast culture
Five kinds of beneficial bacteria of intestinal tract (animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus and lactobacillus delbruockii subspecies bulgaricus) are tested respectively as follows:
1, with 1 of the step 3 of embodiment 1.
2, packet transaction
Experimental group: (Lactobacterium acidophilum, plant lactobacillus and lactobacillus delbruockii subspecies bulgaricus all adopt the MRS substratum in centrifuge tube, to add aseptic culture medium; Animal bifidobacteria adopts the PYG substratum; Thermophilus streptococcus adopts glucose-yeast extract medium) in, the bacteria suspension that obtains of the aseptic yeast culture that obtains of step 2 and step 1, be initial system (liquid amount of centrifuge tube is 50%); The volumn concentration of aseptic yeast culture in initial system is 10%, and the volumn concentration of bacteria suspension in initial system is 10%.
Control group: fermention medium is replaced the aseptic yeast culture in the experimental group, other same experimental group.
Build the stopper of centrifuge tube in each group, place anaerobic jar, cultivate 24h for 37 ℃.
3, cultivate the end back and adopt ultraviolet-uisible spectrophotometer to measure the OD600 (characterizing the biomass of beneficial bacteria of intestinal tract) in total system, the results are shown in Table 3.
The experimental group among table 3 embodiment 3 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.699 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.595 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.513 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.632 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.512 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 1.7 times, the OD600 of Lactobacterium acidophilum and has improved 1.5 times, the OD600 of plant lactobacillus and improved 1.3 times, the OD600 of lactobacillus delbruockii subspecies bulgaricus and improved 1.0 times, the OD600 of thermophilus streptococcus and improved 1.6 times.
The preparation of embodiment 4, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 20g glucose, and nitrogenous source is made up of 2.5g yeast extract paste and 2.5g peptone, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 4.
The experimental group among table 4 embodiment 4 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.489 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.415 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.332 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.461 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.325 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 88.8%, the OD600 of Lactobacterium acidophilum has improved 74.4%, the OD600 of plant lactobacillus has improved 48.9%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 89.7%, the OD600 of thermophilus streptococcus has improved 27.0%.
The preparation of embodiment 5, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 200g glucose, and nitrogenous source is made up of 150g yeast extract paste and 150g peptone, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 5.
The experimental group among table 5 embodiment 5 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.569 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.471 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.403 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.528 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.401 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved that 1.2 times, the OD600 of Lactobacterium acidophilum have improved 97.9%, the OD600 of plant lactobacillus has improved 80.7%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 56.6%, the OD600 of thermophilus streptococcus has improved 1.2 times.
The preparation of embodiment 6, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 100g glucose, and nitrogenous source is made up of 70g yeast extract paste and 70g peptone, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 6.
The experimental group among table 6 embodiment 6 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.529 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.425 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.453 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.522 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.381 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 1.0 times, the OD600 of Lactobacterium acidophilum and has improved 78.6% times, the OD600 of plant lactobacillus and improved 1.0 times, the OD600 of lactobacillus delbruockii subspecies bulgaricus and improved 48.8% times, the OD600 of thermophilus streptococcus and improved 1.15 times.
The preparation of embodiment 7, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 20g sucrose, and nitrogenous source is made up of 2.5g yeast extract paste and 2.5g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 7.
The experimental group among table 7 embodiment 7 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.509 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.415 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.320 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.441 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.316 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 96.5%, the OD600 of Lactobacterium acidophilum has improved 74.4%, the OD600 of plant lactobacillus has improved 43.5%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 23.4%, the OD600 of thermophilus streptococcus has improved 81.5%.
The preparation of embodiment 8, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 200g sucrose, and nitrogenous source is made up of 150g yeast extract paste and 150g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 8.
The experimental group among table 8 embodiment 8 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.602 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.489 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.399 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.540 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.421 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved that 1.32 times, the OD600 of Lactobacterium acidophilum have improved that 1.05 times, the OD600 of plant lactobacillus have improved 78.9%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 64.5%, the OD600 of thermophilus streptococcus has improved 1.22 times.
The preparation of embodiment 9, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 100g sucrose, and nitrogenous source is made up of 70g yeast extract paste and 70g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 9.
The experimental group among table 9 embodiment 9 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.559 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.465 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.373 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.482 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.359 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved that 1.16 times, the OD600 of Lactobacterium acidophilum have improved 95.4%, the OD600 of plant lactobacillus has improved 67.3%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 40.2%, the OD600 of thermophilus streptococcus has improved 98.4%.
The preparation of embodiment 10, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is the 100g molasses, and nitrogenous source is made up of 70g peptone and 70g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 10.
The experimental group among table 10 embodiment 10 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.502 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.410 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.322 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.441 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.320 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 93.8%, the OD600 of Lactobacterium acidophilum has improved 72.3%, the OD600 of plant lactobacillus has improved 44.4%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 25% times, the OD600 of thermophilus streptococcus and improved 81.5%.
The preparation of embodiment 11, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is the 200g molasses, and nitrogenous source is made up of 150g peptone and 150g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 11.
The experimental group among table 11 embodiment 11 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group |
| Animal bifidobacteria | (0.567 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.465 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.470 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.504 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.392 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved that 1.2 times, the OD600 of Lactobacterium acidophilum have improved 95.4%, the OD600 of plant lactobacillus has improved that 1.1 times, the OD600 of lactobacillus delbruockii subspecies bulgaricus have improved 53.1%, the OD600 of thermophilus streptococcus has improved 1.07 times.
The preparation of embodiment 12, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is the 20g molasses, and nitrogenous source is made up of 2.5g peptone and 2.5g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 12.
The experimental group among table 12 embodiment 12 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.452 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.366 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.302 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.401 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.312 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 74.5%, the OD600 of Lactobacterium acidophilum has improved 53.8%, the OD600 of plant lactobacillus has improved 35.4%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 21.9%, the OD600 of thermophilus streptococcus has improved 65.0%.
The preparation of embodiment 13, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 20g glucose, and nitrogenous source is the 5g yeast extract paste, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 13.
The experimental group among table 13 embodiment 13 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.432 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.342 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.285 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.375 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.290 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 66.8%, the OD600 of Lactobacterium acidophilum has improved 43.7%, the OD600 of plant lactobacillus has improved 27.8%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 13.3%, the OD600 of thermophilus streptococcus has improved 54.3%.
The preparation of embodiment 14, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 200g glucose, and nitrogenous source is the 300g yeast extract paste, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 14.
The experimental group among table 14 embodiment 14 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.555 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.490 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.421 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.410 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.344 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved that 1.14 times, the OD600 of Lactobacterium acidophilum have improved that 1.06 times, the OD600 of plant lactobacillus have improved 88.8%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 34.4%, the OD600 of thermophilus streptococcus has improved 68.7%.
The preparation of embodiment 15, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 20g sucrose, and nitrogenous source is the 5g peptone, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 15.
The experimental group among table 15 embodiment 15 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.445 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.358 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.289 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.390 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.295 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 71.8%, the OD600 of Lactobacterium acidophilum has improved 50.4%, the OD600 of plant lactobacillus has improved 29.6%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 15.2%, the OD600 of thermophilus streptococcus has improved 60.5%.
The preparation of embodiment 16, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is a 200g sucrose, and nitrogenous source is the 300g peptone, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 16.
The experimental group among table 16 embodiment 16 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.535 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.452 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.396 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.377 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.310 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved that 1.07 times, the OD600 of Lactobacterium acidophilum have improved 89.9%, the OD600 of plant lactobacillus has improved 77.6%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 21.1%, the OD600 of thermophilus streptococcus has improved 55.1%.
The preparation of embodiment 17, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is the 20g molasses, and nitrogenous source is a 5g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 17.
The experimental group among table 17 embodiment 17 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.405 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.329 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.260 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.355 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.269 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 56.4%, the OD600 of Lactobacterium acidophilum has improved 38.2%, the OD600 of plant lactobacillus has improved 16.6%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 5.1%, the OD600 of thermophilus streptococcus has improved 46.1%.
The preparation of embodiment 18, yeast culture and application
One, culture medium preparation
Seed culture medium (natural pH): with the seed culture medium of embodiment 3.
Fermention medium (pH6.5): carbon source is the 200g molasses, and nitrogenous source is a 300g ammonium sulfate, and other prescription is with the fermention medium of embodiment 3.
Two, the preparation of yeast culture
Step 2 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.
Three, the application of yeast culture
Step 3 with embodiment 3.The result sees table 18.
The experimental group among table 18 embodiment 18 and the OD600 result of control group
| The OD600 result of experimental group | The OD600 result of control group | |
| Animal bifidobacteria | (0.505 the result after diluting 4 times) | (0.259 the result after diluting 4 times) |
| Lactobacterium acidophilum | (0.421 the result after diluting 11 times) | (0.238 the result after diluting 11 times) |
| Plant lactobacillus | (0.360 the result after diluting 11 times) | (0.223 the result after diluting 11 times) |
| Thermophilus streptococcus | (0.352 the result after diluting 11 times) | (0.243 the result after diluting 11 times) |
| Lactobacillus delbruockii subspecies bulgaricus | (0.270 the result after diluting 11 times) | (0.256 the result after diluting 11 times) |
Compare with control group; After adding aseptic yeast culture, the OD600 of animal bifidobacteria has improved 95.0%, the OD600 of Lactobacterium acidophilum has improved 76.9%, the OD600 of plant lactobacillus has improved 61.4%, the OD600 of lactobacillus delbruockii subspecies bulgaricus has improved 5.5%, the OD600 of thermophilus streptococcus has improved 44.9%.
Claims (10)
1. method for preparing yeast culture is CGMCC to be numbered 2.1882 yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ferment, and obtains yeast culture.
2. the method for claim 1, it is characterized in that: said fermentation comprises the steps:
(1) said yeast saccharomyces cerevisiae is seeded to seed culture medium, 20-50 ℃, 100-200rpm jolting cultivation 18-40h obtain seed liquor;
(2) seed liquor with step (1) is seeded to fermention medium, and 20-50 ℃ leaves standstill cultivation 30-60h.
3. method as claimed in claim 2 is characterized in that: the preparation method of said fermention medium is following: get 20-200g carbon source, 5-300g nitrogenous source, 0.1-10g KH
2PO
4, 0.1-10g MgSO
47H
2O, 0.1-4g MnSO
4H
2O, 0.1-4g CaCl
2, 0.1-4g CuSO
45H
2O, 0.1-4g ZnSO
47H
2O and 0.1-4g FeSO
44H
2O is with water dissolution and be settled to 1L.
4. method as claimed in claim 3 is characterized in that: said carbon source is glucose and/or the carbohydrate that contains sucrose; Said nitrogenous source is yeast extract paste and/or peptone and/or ammonium sulfate; The said carbohydrate that contains sucrose is specially sucrose or molasses.
5. like arbitrary described method in the claim 2 to 4, it is characterized in that: the preparation method of said seed culture medium is specific as follows: get 10-40g glucose, 5-40g yeast powder, 10-40g peptone and 0.1-4g MnSO
4H
2O is with water dissolution and be settled to 1L.
6. like arbitrary described method in the claim 2 to 5, it is characterized in that: when said seed liquor was seeded to said fermention medium, the volume proportion of said seed liquor and said fermention medium was 1-30: 100.
7. the yeast culture that arbitrary said method prepares in the claim 1 to 6.
8. the said yeast culture of claim 7 is at (a) as follows or the application (b):
(a) promote beneficial bacteria of intestinal tract propagation;
(b) preparation promotes the preparation of beneficial bacteria of intestinal tract propagation.
9. a method of cultivating beneficial bacteria of intestinal tract is at the substratum said beneficial bacteria of intestinal tract of adding and the said yeast culture of claim 7 that are used for cultivating said beneficial bacteria of intestinal tract, obtains the initial incubation system, cultivates then.
10. application as claimed in claim 8 or method as claimed in claim 9 is characterized in that: said beneficial bacteria of intestinal tract is animal bifidobacteria, Lactobacterium acidophilum, plant lactobacillus, thermophilus streptococcus or lactobacillus delbruockii subspecies bulgaricus.
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| CN103834602A (en) * | 2014-03-21 | 2014-06-04 | 南京千人慧生物科技有限公司 | High density fermentation medium for lactic acid bacteria |
| CN104195180A (en) * | 2014-08-08 | 2014-12-10 | 广西湘桂酵母科技有限公司 | Method for fermentation production of high density yeast extract emulsion |
| CN106912602A (en) * | 2017-01-24 | 2017-07-04 | 深圳市大百汇技术有限公司 | A kind of fermented dairy by Lactobacillus acidophilus of laetarius volemus polysaccharide and preparation method thereof |
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| CN102113629A (en) * | 2009-12-31 | 2011-07-06 | 内蒙古伊利实业集团股份有限公司 | Method for preparing dairy cow feed by microbial fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102113629A (en) * | 2009-12-31 | 2011-07-06 | 内蒙古伊利实业集团股份有限公司 | Method for preparing dairy cow feed by microbial fermentation |
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| A. VAUGHAN MARTINI & A. MARTINI: "Three newly delimited species of Saccharomyces sensu stricto.", 《ANTONIE VAN LEEUWENHOEK》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103834602A (en) * | 2014-03-21 | 2014-06-04 | 南京千人慧生物科技有限公司 | High density fermentation medium for lactic acid bacteria |
| CN104195180A (en) * | 2014-08-08 | 2014-12-10 | 广西湘桂酵母科技有限公司 | Method for fermentation production of high density yeast extract emulsion |
| CN106912602A (en) * | 2017-01-24 | 2017-07-04 | 深圳市大百汇技术有限公司 | A kind of fermented dairy by Lactobacillus acidophilus of laetarius volemus polysaccharide and preparation method thereof |
| CN106912602B (en) * | 2017-01-24 | 2020-10-02 | 深圳市大百汇技术有限公司 | Lactobacillus acidophilus fermented milk containing succulent lactarius polysaccharide and preparation method of lactobacillus acidophilus fermented milk |
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