CN102558390A - Chitohexaose chromium complex and preparation method of chitohexaose chromium complex - Google Patents
Chitohexaose chromium complex and preparation method of chitohexaose chromium complex Download PDFInfo
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- CN102558390A CN102558390A CN2011104491032A CN201110449103A CN102558390A CN 102558390 A CN102558390 A CN 102558390A CN 2011104491032 A CN2011104491032 A CN 2011104491032A CN 201110449103 A CN201110449103 A CN 201110449103A CN 102558390 A CN102558390 A CN 102558390A
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- chitohexaose
- chromium
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Abstract
The invention provides a chitohexaose chromium complex and a preparation method of the chitohexaose chromium complex. Chitohexaose chromium is a complex of six-membered-ring chitosan oligosaccharide and chromium trichloride, has average molecular weight of 1000-1500, has deacetylation degree more than 90%, and is the chitohexaose chromium complex formed by coordination of chitosan oligosaccharide and Cr<3+>. According to the invention, the chitohexaose chromium complex is prepared, and the optimum reaction conditions for preparing the chitohexaose chromium complex are determined. Chromium utilization rate can be improved by using the chitohexaose chromium complex, and the chitohexaose chromium complex is expected to have important application value in food and feed additive fields; the equipment is simple, the operational conditions are easy to control, and the production cost is low; and the obtained chitohexaose chromium complex is high in conversion rate.
Description
Technical field
The invention belongs to the field of chemical synthesis, be specifically related to shell six sugared chromic compounds and preparation method thereof.
Background technology
Chromium is the essential trace element of humans and animals.Nineteen fifty-nine, United States Medicine man Mertz and Schwarz find and confirm Gr
3+Be the active factor in the glucose tolerance factor (GTF), chromium (chromium) is body lipid, protein and the necessary trace element of glucide homergy.
Oligochitosan (Chitosan oligosaccharide) claims that also chitin oligo saccharide, formal name used at school are β-1, and 4-oligomerization-glycamine is called as " the 6th key element of life ", is present unique in the world positively charged ion animal fibre material that has the free amine group basic group.The research proof: oligochitosan not only has the raising immunity, promotes liver spleen antibody to form, and the effect of profitable strains such as propagation bifidus bacillus, milk-acid bacteria promotes the effect of mineral substance absorptions such as calcium in addition, is a kind of multi-functional biologically active substance.Do not utilize oligochitosan and inorganic chromium relevant report but see as yet at present for feedstock production shell six sugared chromic compounds.Material of the present invention is expected to as food or fodder additives to improve the utilization ratio of chromium.
Summary of the invention
The purpose of this invention is to provide a kind of shell six sugared chromic compounds and preparation method thereof.Shell six sugared chromic compounds and inorganic chromium specific energy mutually improve the utilization ratio of chromium, and it not only can be used as the chromium source in food and the feed, and the oligochitosan of biologically active can also be provided simultaneously, can bring into play physiologically active separately, and the two has synergistic effect again.
For realizing above-mentioned purpose; Technical scheme of the present invention is: a kind of shell six sugared chromic compounds, be the oligochitosan of hydrogenation of six carbocyclic and the title complex of chromium trichloride, and the molecular-weight average of title complex is about 1000～1500; Deacetylation＞90% is owing to contain a large amount of dissociating-NH in the oligochitosan molecule
2, and-NH
2The ortho position is-OH, can borrow hydrogen bond, also can borrow sat linkage to form and have similar cancellated cage type molecule, thereby metals ion is had stable coordination.Oligochitosan and Gr
3+The shell six sugared chromic compounds that coordination forms, its general structure fragment is following:
Shell six sugared chromic compounds can prolong the dextran amine chain direction and dextran amine chain vertical direction extends to form two-dirnentional structure.
When above-mentioned shell six sugared chromic compounds prepared, reaction conditions was a normal temperature and pressure, and its concrete preparation process is following:
1) oligochitosan and chromium chloride being processed mass concentration ratio respectively is 3: 1 the aqueous solution;
2) mix the oligochitosan aqueous solution and chromium chloride solution equal-volume, using mass concentration then is that 1% ammoniacal liquor transfers to pH9.0 with mixing solutions;
3), throw out is washed 1～5 time with zero(ppm) water the mixing solutions suction filtration behind the chelatropic reaction;
4) moisture is removed in the throw out oven dry;
5) obtain blackish green pulverulent solids and be shell six sugared chromic compounds.
The shell six sugared chromic compounds that take a morsel are put into organic solvent methyl alcohol commonly used, ethanol, ether, acetone, and the result finds that shell six sugared chromic compounds are all insoluble.
Description of drawings
The infrared spectrogram of Fig. 1, shell six sugared chromic compounds.
The infrared spectrogram of Fig. 2, oligochitosan.
Embodiment
Through specific embodiment the present invention is described further below.
Take by weighing the 1.5g oligochitosan, the 0.5g chromium chloride is put into beaker respectively, and each adds stirring and dissolving behind the 25ml zero(ppm) water; Then two kinds of solution equal-volumes are mixed, fully stir and regulate ph value and slowly rise to 9.0 through the ammonia soln of 1% concentration, about behind several minutes chelating; Suction filtration; And, remove residual filtrating with distilled water flushing 3 times, throw out is placed 70 ℃ baking oven to remove in 6 hours to obtain blackish green pulverulent solids behind the moisture be shell six sugared chromic compounds.
Through comparison diagram 1 and Fig. 2, can find out, after infrared measurement chromium chloride and oligochitosan reaction, be positioned at about 600cm
-1The place ,-NH
2Absorption peak dies down, and explains in the oligochitosan molecule-NH
2Participated in chelatropic reaction; Be positioned at secondary hydroxyl 1072cm
-1To the high frequency displacement and obviously weaken, explain-OH also participated in chelatropic reaction; Be positioned at 1635cm
-1About-the bimodal disappearance of C=O key, also explained-two keys of C=O and metal ion-chelant, formed-C-O-Cr.The ir spectra test-results shows that oligochitosan and chromium ion have formed stable chelate.
Claims (3)
1. shell six sugared chromic compounds is characterized in that, it is the oligochitosan of hydrogenation of six carbocyclic and the title complex of chromium trichloride; Molecular-weight average is 1000～1500; Deacetylation＞90%, the shell six sugared chromic compounds that oligochitosan and Cr3+ coordination form, its general structure fragment is following:
Shell six sugared chromic compounds can prolong the dextran amine chain direction and dextran amine chain vertical direction extends to form two-dirnentional structure.
2. the preparation method of the described shell six sugared chromic compounds of claim 1 is characterized in that the reaction conditions during preparation is a normal temperature and pressure, and concrete steps are following:
1) oligochitosan of hydrogenation of six carbocyclic and chromium chloride being processed mass concentration ratio respectively is 3: 1 the aqueous solution;
2) mix the oligochitosan of hydrogenation of six carbocyclic and chromium chloride solution equal-volume, using mass concentration then is that 1% ammoniacal liquor transfers to pH9.0;
3), throw out is washed 1～5 time with zero(ppm) water the mixing solutions suction filtration behind the chelatropic reaction;
4) moisture is removed in the throw out oven dry, got blackish green pulverulent solids.
3. according to the said shell six sugared chromic compounds of claim 1, it is characterized in that, be blackish green pulverulent solids, and organic solvent water insoluble and commonly used, described organic solvent commonly used is methyl alcohol, ethanol, ether or acetone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104491032A CN102558390A (en) | 2011-12-28 | 2011-12-28 | Chitohexaose chromium complex and preparation method of chitohexaose chromium complex |
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|---|---|---|---|
| CN2011104491032A CN102558390A (en) | 2011-12-28 | 2011-12-28 | Chitohexaose chromium complex and preparation method of chitohexaose chromium complex |
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| CN102558390A true CN102558390A (en) | 2012-07-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103059154A (en) * | 2012-11-29 | 2013-04-24 | 滨州学院 | Fungal glucan oligomer chrome complex and preparation method thereof |
-
2011
- 2011-12-28 CN CN2011104491032A patent/CN102558390A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103059154A (en) * | 2012-11-29 | 2013-04-24 | 滨州学院 | Fungal glucan oligomer chrome complex and preparation method thereof |
| CN103059154B (en) * | 2012-11-29 | 2015-03-11 | 滨州学院 | Fungal glucan oligomer chrome complex and preparation method thereof |
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Application publication date: 20120711 |