CN101849678A - Chitosan oligosaccharide chelating Zn and preparation method thereof - Google Patents
Chitosan oligosaccharide chelating Zn and preparation method thereof Download PDFInfo
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- CN101849678A CN101849678A CN200910011019A CN200910011019A CN101849678A CN 101849678 A CN101849678 A CN 101849678A CN 200910011019 A CN200910011019 A CN 200910011019A CN 200910011019 A CN200910011019 A CN 200910011019A CN 101849678 A CN101849678 A CN 101849678A
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- chitosan oligosaccharide
- chelating
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Abstract
The invention discloses a chitosan oligosaccharide chelating Zn and a preparation method thereof. At present, the report of preparing the chitosan oligosaccharide chelating Zn by the interaction of chitosan oligosaccharide and zinc sulfate is not available yet. The invention can be used for preparing the chitosan oligosaccharide chelating Zn, and confirms the best reaction condition of the preparation of the chitosan oligosaccharide chelating Zn. The chitosan oligosaccharide chelating Zn can improve the utilization rate of Zn, is expected to have important application valve in the fields of food and feed additive. The invention has simple equipment, easily controlled operation condition and low production cost, and the obtained chitosan oligosaccharide chelating Zn has high conversion rate.
Description
Technical field
The present invention relates to chitosan oligosaccharide chelating Zn and preparation method thereof.
Background technology
Zinc is the constituent of more than 40 kind of metalloenzyme in the body and the activity factor of 200 plurality of enzymes, and wide participation nucleic acid and protein are synthetic, energetic supersession, redox, cell and humoral immunity process.Add zinc in food and the animal feed at present to based on inorganic zinc sources such as zinc sulfate, zinc oxide, but so the inorganic zinc utilization rate is crossed low 90% zinc all is excreted, not only waste is serious but also contaminated environment in ight soil.A lot of in recent years reports show that amino-acid metal chelate can significantly improve the metallic element utilization rate.
Chitosan oligosaccharide (Chitosan oligosaccharide) claims that also chitin oligo saccharide, formal name used at school are β-1, and 4-oligomerization-gucosamine is called as " the 6th key element of life ", is present unique in the world cation animal origin material that has the free amine group basic group.Studies have shown that: chitosan oligosaccharide not only has the raising immunity, promotes liver spleen antibody to form, and the effect of profitable strains such as propagation Bifidobacterium, lactic acid bacteria promotes the effect of mineral matter absorptions such as calcium in addition, is a kind of multi-functional bioactivator.Utilize chitosan oligosaccharide and inorganic zinc relevant report but have not yet to see for the feedstock production chitosan oligosaccharide chelating Zn.Material of the present invention is expected to as food or feed addictive to improve the utilization rate of zinc.
Summary of the invention
The purpose of this invention is to provide a kind of chitosan oligosaccharide chelating Zn and preparation thereof.Chitosan oligosaccharide chelating Zn and inorganic zinc specific energy mutually improve the utilization rate of zinc, and it not only can be used as the zinc source in food and the feed, and the chitosan oligosaccharide of biologically active can also be provided simultaneously, can bring into play physiologically active separately, and the two has cooperative effect again.
For achieving the above object, technical scheme of the present invention is: a kind of chitosan oligosaccharide chelating Zn: it is characterized by mixed composition, mean molecule quantity is about 3000-4000, and deacetylation＞90% is owing to contain a large amount of dissociating-NH in the chitosan oligosaccharide molecule
2, and-NH
2The ortho position is-OH, can borrow hydrogen bond, also can borrow sat linkage to form and have similar cancellated cage type molecule, thereby metal ion is had stable coordination.Chitosan oligosaccharide and Zn
2+The chitosan oligosaccharide chelating Zn that coordination forms, its general structure fragment is as follows:
Chitosan oligosaccharide chelating Zn can prolong the dextran amine chain direction and dextran amine chain vertical direction extends to form two-dimensional structure.
The preparation of described chitosan oligosaccharide chelating Zn, reaction condition are normal temperature and pressure, and its step is as follows:
1, chitosan oligosaccharide and zinc sulfate being made mass concentration ratio respectively is 2: 1 the equal-volume aqueous solution.As: chitosan oligosaccharide concentration be 4% and zinc sulfate concentration be each 25ml of the aqueous solution of 2%.
2, chitosan oligosaccharide and zinc sulfate solution equal-volume being mixed, is that 1% ammoniacal liquor transfers to pH6.0-7.0 with mass concentration then.
3, the mixed solution suction filtration behind the chelatropic reaction, sediment is washed 1-5 time with distilled water.
4, moisture is removed in the sediment oven dry.
5, obtain the khaki pulverulent solids and be chitosan oligosaccharide chelating Zn.
6, the chitosan oligosaccharide chelating Zn that takes a morsel is put into organic solvent methyl alcohol commonly used, ethanol, ether, acetone, found that chitosan oligosaccharide chelating Zn is all insoluble.
Description of drawings
Fig. 1 is the infrared spectrum comparison diagram of chitosan oligosaccharide chelating Zn and chitosan oligosaccharide, and figure center line a represents chitosan oligosaccharide, and line b represents chitosan oligosaccharide chelating Zn.
The specific embodiment
Below by example the chitosan oligosaccharide chelating Zn preparation method that this invention provides is described further.
Take by weighing the 1g chitosan oligosaccharide, 0.5g zinc chloride is put into beaker respectively, each adds stirring and dissolving behind the 25ml distilled water, then two kinds of solution equal-volumes are mixed, fully stir and regulate ph value and slowly rise to 6.0-7.0 by the ammonia spirit of 1% concentration, about behind several minutes chelating, suction filtration, and, remove residual filtrate with distilled water flushing 1-5 time, sediment is placed to remove in baking oven 5-6 hour of 70 ℃ obtain green flour powder solid behind the moisture and be chitosan oligosaccharide chelating Zn.
Shown in Figure 1, after infrared spectrum measurement zinc chloride and chitosan oligosaccharide reaction, be positioned at about 600cm
-1The place ,-NH
2Absworption peak dies down, and illustrates in the chitosan oligosaccharide molecule-NH
2Participated in chelatropic reaction; Be positioned at secondary hydroxyl 1079cm
-1To the high frequency displacement and obviously weaken, illustrate-OH also participated in chelatropic reaction; Be positioned at 1600cm
-1About-the bimodal disappearance of C=O key, also illustrated-two keys of C=O and metal ion-chelant, formed-C-O-Zn.The infrared spectrum result of the test shows that chitosan oligosaccharide and zinc ion have formed stable chelate.
Claims (3)
1. a chitosan oligosaccharide chelating Zn is characterized in that, it is a mixed composition, and mean molecule quantity is about 3000-4000, deacetylation＞90%, chitosan oligosaccharide and Zn
2+The chitosan oligosaccharide chelating Zn that coordination forms, its general structure fragment is as follows:
Chitosan oligosaccharide chelating Zn can prolong the dextran amine chain direction and dextran amine chain vertical direction extends to form two-dimensional structure.
2. sting the preparation of closing zinc according to the described chitosan oligosaccharide of claim 1, it is characterized in that, reaction condition is a normal temperature and pressure, and its step is as follows:
1. chitosan oligosaccharide and zinc sulfate are made mass concentration ratio respectively and are 2: 1 the equal-volume aqueous solution,
2. chitosan oligosaccharide and zinc sulfate solution equal-volume being mixed, is that 1% ammoniacal liquor transfers to pH6.0-7.0 with mass concentration then,
3. the mixed solution suction filtration behind the chelatropic reaction, sediment is washed 1-5 time with distilled water,
4. moisture is removed in the sediment oven dry,
5. obtain the khaki pulverulent solids and be chitosan oligosaccharide chelating Zn.
3. according to the described chitosan oligosaccharide chelating Zn of claim 1, it is characterized in that its physical features is as follows:
1. yellow powder shape solid,
2. organic solvent (as methyl alcohol, ethanol, ether, acetone) water insoluble and commonly used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910011019A CN101849678A (en) | 2009-04-02 | 2009-04-02 | Chitosan oligosaccharide chelating Zn and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910011019A CN101849678A (en) | 2009-04-02 | 2009-04-02 | Chitosan oligosaccharide chelating Zn and preparation method thereof |
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| CN101849678A true CN101849678A (en) | 2010-10-06 |
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| CN200910011019A Pending CN101849678A (en) | 2009-04-02 | 2009-04-02 | Chitosan oligosaccharide chelating Zn and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351959A (en) * | 2011-08-29 | 2012-02-15 | 河南中医学院 | Preparation method and application of multiflower knotweed vine polysaccharide chelate zinc |
| CN104543389A (en) * | 2014-12-25 | 2015-04-29 | 青岛颂田生物技术有限公司 | Efficient green chitosan oligosaccharide compound agricultural additive |
| CN104672344A (en) * | 2015-03-25 | 2015-06-03 | 福建农林大学 | Enteromorpha functional oligosaccharide zinc and preparation method thereof |
| CN109354633A (en) * | 2018-11-14 | 2019-02-19 | 黄河三角洲京博化工研究院有限公司 | A kind of chitosan oligosaccharide chelates of zinc and its preparation method and application |
| CN114376077A (en) * | 2022-01-18 | 2022-04-22 | 湖南德邦生物科技有限公司 | Preparation method of chitosan oligosaccharide chelated zinc |
-
2009
- 2009-04-02 CN CN200910011019A patent/CN101849678A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351959A (en) * | 2011-08-29 | 2012-02-15 | 河南中医学院 | Preparation method and application of multiflower knotweed vine polysaccharide chelate zinc |
| CN104543389A (en) * | 2014-12-25 | 2015-04-29 | 青岛颂田生物技术有限公司 | Efficient green chitosan oligosaccharide compound agricultural additive |
| CN104672344A (en) * | 2015-03-25 | 2015-06-03 | 福建农林大学 | Enteromorpha functional oligosaccharide zinc and preparation method thereof |
| CN109354633A (en) * | 2018-11-14 | 2019-02-19 | 黄河三角洲京博化工研究院有限公司 | A kind of chitosan oligosaccharide chelates of zinc and its preparation method and application |
| CN114376077A (en) * | 2022-01-18 | 2022-04-22 | 湖南德邦生物科技有限公司 | Preparation method of chitosan oligosaccharide chelated zinc |
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Open date: 20101006 |