CN102550794A - Method for extracting cottonseed protein from cottonseed meal - Google Patents
Method for extracting cottonseed protein from cottonseed meal Download PDFInfo
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- CN102550794A CN102550794A CN2012100026820A CN201210002682A CN102550794A CN 102550794 A CN102550794 A CN 102550794A CN 2012100026820 A CN2012100026820 A CN 2012100026820A CN 201210002682 A CN201210002682 A CN 201210002682A CN 102550794 A CN102550794 A CN 102550794A
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Abstract
The invention relates to a method for extracting cottonseed protein. The method comprises the following steps: (1), smashing cottonseeds; (2), treating the cottonseeds with ultrasonic waves; (3), extracting active enzyme in cotton bollworms; (4), performing enzymolysis to cottonseed meal; (5), increasing the temperature, inactivating the enzyme, and centrifugating to obtain supernatant liquid and filter residue; (6), adding water to flush the filter residue, and mixing the liquid after the filter residue is flushed with the supernatant liquid obtained in the step (3); and (7), removing impurities from the mixed supernatant liquid through a micro filter membrane, condensing through a nanofiltration membrane, and drying to obtain the powdery cottonseed protein. The method provided by the invention can ensure a higher extraction rate in the production of cottonseed protein, can enable the product to have a stronger functional activity, and meanwhile solves the problem that the conventional biological enzymolysis process scheme requires to purchase costly protease, resulting in a high production cost.
Description
Technical field
The present invention relates to a kind of method of extracting cottonseed protein, relating generally to a kind of is raw material with the cottonseed dregs of rice, is enzyme preparation and combines ultrasonic technology and the method for membrane separation technique extraction cottonseed protein with bollworm enteron aisle enzyme.
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Background technology
Cottonseed has extracted in the cottonseed dregs of rice after the grease and has contained amounts of protein, and its content is about about 50%, is a kind of very abundant vegetable protein resource.The character and the soybean protein of cottonseed protein are similar, also be a kind ofly be of high nutritive value, colory protein food or feed protein source.
Protein in the cottonseed dregs of rice mainly is divided into three kinds; 2S protein is free in outside the cell membrane; Account for about 15%, remaining about 85% 7S, 12S protein are present in the cell membrane, because the cell membrane of cottonseed protein is very tough and tensile; Employing is similar to the heavy technology of the molten acid of alkali that big dregs of beans extracts soybean protein, and the recovery rate of cottonseed protein is not high.If adopt the way of highly basic, weak acid, high temperature to break its cell membrane, because reaction condition is strong, the amino acid sequence of constitutive protein matter can be destroyed, and protein will lose its function and activity.Adopt enzymolysis process, break cell membrane and aminosal, be a kind of method of comparatively desirable extraction cottonseed protein, but, cause production cost to be difficult to descend because commercially available protease preparation price is higher through biological agent.As: CN102206290A discloses a kind of method of from the cottonseed dregs of rice, extracting the cottonseed protein oligopeptide, and the cottonseed dregs of rice are pulverized, and warm water is carried in carrying out, centrifugation; Slag after water is lifted from the heart and separated dissolves carrying out alkali, and alkali protease carries out enzymolysis, decolouring; The enzyme that goes out, centrifugation, supernatant carries out the micro-filtration membrane removal of impurities; NF membrane concentrates, and last concentrate obtains the cottonseed protein oligopeptide again through spray-drying.CN101709317A discloses a kind of method of from the low temperature desolventizing cottonseed dregs of rice, extracting the cottonseed protein isolate, comprising: 1. the raw material low temperature desolventizing cottonseed dregs of rice are pulverized, added the entry mixing, add cellulose complex enzyme again and carry out enzyme digestion reaction; 2. regulate pH to 11.5～12.5, solution is heated to 90～105 ℃ carries out the alkaline thermal hydrolysis reaction; 3. the alkaline thermal hydrolysis reacted solution is centrifugalized; 4. regulate pH and make protein precipitation to the isoelectric point of protein, centrifugation; 5. the centrifugal protein that obtains is dissolved in water, transfers pH to neutral, drying obtains the cottonseed protein isolate.Obviously, the method for the disclosed extraction cottonseed protein of above-mentioned document has all adopted commercially available protease preparation, causes production cost high.Therefore seeking a kind of enzyme cheaply is used for the hydrolysis cottonseed protein, and combines other advanced technologies extraction cottonseed proteins to have crucial meaning.
In addition, genus heliothis Lepidoptera Noctuidae extensively is distributed in China and reaches all over the world, and all there is existence in Chinese cotton region and growing vegetables district.Bollworm is an important borer pest of cotton cotton buds and bolls phase, and main moth food flower bud, flower, bell are also got the food tender leaf.The enzyme that contains the cottonseed protein of degrading in the bollworm enteron aisle can be converted into the high molecular weight protein in the cottonseed micromolecular albumen and amino acid and be beneficial to the absorption of self.Because of bollworm virus is a kind of insect pesticide; Its prevention effect is good, lasting medicine; Without any side effects to other biological, be the insect viruses biological pesticide of first registration in the world, so the breed problem of bollworm receives domestic and international researcher's extensive concern; Solve bollworm group support technical barrier at present, realized large-scale breeding.
Ultrasonic wave extraction is a kind of widely used assisted extraction method, utilizes that ultrasonic wave produces at a high speed, strong cavitation effect and stirring action, destroys the eucaryotic cell structure of plant, can shorten extraction time, improves the recovery rate of target product.Membrane separation technique is to utilize film that the selection permeability difference of each composition of Combination fluid foods is realized separation, purifying, concentrated new separation technology; Have advantages such as efficient, energy-saving and environmental protection; The film separation process is a kind of physical process; Material can not produce chemical phase transformation in separation process, membrane separation technique is used widely in fields such as biology, medicine, chemical industry, environmental protection at present.
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Summary of the invention
In order to make the production of cottonseed protein have higher recovery rate; And product has stronger functional activity; Solve existing biological enzymolysis process program simultaneously and need give a long price for protease; Cause the higher problem of production cost, the present invention provides a kind of organized enzyme that utilizes the bollworm intestinal secretion as enzyme preparation, in conjunction with the method for ultrasonic technology and membrane separation technique extraction cottonseed protein.
For realizing the object of the invention, its technical scheme such as following step are said:
A kind of method of from the cottonseed dregs of rice, extracting cottonseed protein comprises the steps:
(1) the cottonseed dregs of rice is pulverized;
(2) adding accounts for 8-12 times of water of cottonseed dregs of rice weight, is warming up to 40～50 ℃, and 25～35kHZ frequency ultrasonic wave is handled 40～60min;
(3) with bollworm clean, smash to pieces, homogenate, add 4-6 times of water dilution of worm slurry weight, adjust pH 5.0 ~ 6.0,30～40kHZ frequency ultrasonic wave extracts 80-120s, centrifugation back supernatant is a bollworm organized enzyme liquid;
(4) after the cottonseed dregs of rice ultrasonic Treatment, carry out enzymolysis again, temperature is adjusted into 55～60 ℃, the pH value is 7.5～8.0, adds the bollworm organized enzyme liquid that the step (3) account for cottonseed dregs of rice weight 5～8% obtains, enzymolysis 1～2h;
(5) after enzymolysis is accomplished, be warming up to the enzyme that goes out more than 85 ℃, centrifugation obtains supernatant and filter residue respectively then;
(6) filter residue that obtains in the step (5) is added the 4-6 times of water that accounts for cottonseed dregs of rice weight again, wash once, the supernatant that obtains in water lotion and the step (5) merges;
(7) supernatant after the above-mentioned merging is carried out removal of impurities through the micro-filtration membrane device, obtain micro-filtration membrane filtrating, the condition of work of micro-filtration membrane device is: pressure 0.2～0.25MPa, and 40～50 ℃ of temperature, flow is 90L/min, the micro-filtrate membrane filtration aperture is 0.1～1.0um;
(8) the micro-filtration membrane filtrating that obtains in the step (7) is concentrated through the NF membrane device again; Obtain the NF membrane concentrate, the condition of work of NF membrane device is: pressure 1.5～2MPa, 40～50 ℃ of temperature; Flow 20L/min, NF membrane pore size filter are 0.1～1.0nm;
(9) again that the NF membrane concentrate that obtains in the step (8) is dry, promptly obtain the powdery cottonseed protein.
Described a kind of method of from the cottonseed dregs of rice, extracting cottonseed protein, when wherein extracting the bollworm organized enzyme in the step (3), the worm slurry is 1:5 with the weight ratio of amount of water, and the pH value is 5.0 ~ 6.0, and ultrasonic frequency is 30～40kHZ, and extraction time is 100s.
Compared with prior art, the method for from the cottonseed dregs of rice, extracting cottonseed protein that the present invention relates to have the following advantages and obvious improvement property:
(1) utilized the bollworm extract cottonseed protein to be degraded, increased the solubility of albumen, reduced the production cost of enzymolysis and extraction technology as enzyme preparation.
(2) adopt the ultrasonic-assisted extraction technology to extract cottonseed protein, the recovery rate of cottonseed protein improves 3～5% than prior art scheme, and extraction time can shorten about 20%.
(3) adopt membrane separation technique to carry out purifying, concentrate, yield is high, and the cottonseed protein purity that obtains is more than 90%, and molecular weight mainly is distributed in about 1000Dal.
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The specific embodiment
For the present invention is carried out detailed explanation, the embodiment that at present is listed below further introduces the present invention program, the cottonseed dregs of rice of selecting for use among the embodiment, and recording its protein average content is 49.5%.
Embodiment 1:
Step (1), get the 10kg cottonseed dregs of rice, pulverize, granularity is 60 orders;
Step (2), adding 100kg water are warming up to 40 ℃, and the 25kHZ frequency ultrasonic wave is handled 40min;
Step (3), get bollworm 150g, clean, smash to pieces, homogenate, add 5 times of water dilutions of worm slurry weight, adjust pH is 5.0, and the 30kHZ frequency ultrasonic wave extracts 100s, and centrifugation back supernatant is an organized enzyme liquid;
Step (4), the system that will pass through after step (2) ultrasonic Treatment are warming up to 55 ℃, regulate pH value to 7.5, the bollworm organized enzyme extract 500g that adding step (3) obtains, enzymolysis 1h;
Step (5), will pass through the system that obtains behind step (4) enzymolysis and be warming up to 85 ℃ of enzymes that go out, centrifugation obtains supernatant and filter residue respectively then;
Step (6), the filter residue that obtains in the step (5) is added 5 times of water that account for cottonseed dregs of rice weight again, wash once, the supernatant that obtains in water lotion and the step (5) merges;
Step (7), the clear liquid after the above-mentioned merging is carried out removal of impurities through the micro-filtration membrane device, obtain micro-filtration membrane filtrating, the condition of work of micro-filtration membrane device is: pressure 0.2～0.25MPa, and 40～50 ℃ of temperature, flow is 90L/min, the micro-filtrate membrane filtration aperture is 0.6um;
Step (8), the micro-filtration membrane that obtains in the step (7) filtrating is concentrated through the NF membrane device again; Obtain the NF membrane concentrate, the condition of work of NF membrane device is: pressure 1.5～2MPa, 40～50 ℃ of temperature; Flow 20L/min, NF membrane pore size filter are 0.8nm;
Step (9), the NF membrane concentrate that step (8) is obtained are dry, can obtain powdery cottonseed protein 5.032kg, are 90.5% through measuring protein content, and protein extracting ratio is 92%.
Embodiment 2:
Step (1): get the 10kg cottonseed dregs of rice, pulverize, granularity is 100 orders;
Step (2): add 100kg water, be warming up to 50 ℃, the 35kHZ frequency ultrasonic wave is handled 60min;
Step (3): get bollworm 200g, clean, smash to pieces, homogenate, add 5 times of water dilutions of worm slurry weight, adjust pH is 6.0, and the 40kHZ frequency ultrasonic wave extracts 100s, and centrifugation back supernatant is an organized enzyme liquid;
Step (4): the system that will pass through after step (2) ultrasonic Treatment is warming up to 60 ℃, regulates pH value to 8.0, the bollworm organized enzyme extract 800g that adding step (3) obtains, enzymolysis 2h;
Step (5): will pass through the system that obtains behind step (4) enzymolysis and be warming up to 85 ℃ of enzymes that go out, centrifugation obtains supernatant and filter residue respectively then;
Step (6): the filter residue that obtains in the step (5) is added 5 times of water that account for cottonseed dregs of rice weight again, wash once, the supernatant that obtains in water lotion and the step (5) merges;
Step (7): the clear liquid after the above-mentioned merging is carried out removal of impurities through the micro-filtration membrane device, obtain micro-filtration membrane filtrating, the condition of work of micro-filtration membrane device is: pressure 0.2～0.25MPa, and 40～50 ℃ of temperature, flow is 90L/min, the micro-filtrate membrane filtration aperture is 0.4um;
Step (8): the micro-filtration membrane filtrating that obtains in the step (7) is concentrated through the NF membrane device again; Obtain the NF membrane concentrate, the condition of work of NF membrane device is: pressure 1.5～2MPa, 40～50 ℃ of temperature; Flow 20L/min, NF membrane pore size filter are 0.2nm;
Step (9): the NF membrane concentrate that step (8) obtains is dry, can obtain powdery cottonseed protein 5.075kg, be 91.2% through measuring protein content, protein extracting ratio is 93.5%.
Claims (2)
1. a method of from the cottonseed dregs of rice, extracting cottonseed protein comprises the steps:
(1) the cottonseed dregs of rice is pulverized;
(2) adding accounts for 8-12 times of water of cottonseed dregs of rice weight, is warming up to 40～50 ℃, and 25～35kHZ frequency ultrasonic wave is handled 40～60min;
(3) with bollworm clean, smash to pieces, homogenate, add 4-6 times of water dilution of worm slurry weight, adjust pH 5.0 ~ 6.0,30～40kHZ frequency ultrasonic wave extracts 80-120s, centrifugation back supernatant is a bollworm organized enzyme liquid;
(4) after the cottonseed dregs of rice ultrasonic Treatment, carry out enzymolysis again, temperature is adjusted into 55～60 ℃, the pH value is 7.5～8.0, adds the bollworm organized enzyme liquid that the step (3) account for cottonseed dregs of rice weight 5～8% obtains, enzymolysis 1～2h;
(5) after enzymolysis is accomplished, be warming up to the enzyme that goes out more than 85 ℃, centrifugation obtains supernatant and filter residue respectively then;
(6) filter residue that obtains in the step (5) is added the 4-6 times of water that accounts for cottonseed dregs of rice weight again, wash once, the supernatant that obtains in water lotion and the step (5) merges;
(7) supernatant after the above-mentioned merging is carried out removal of impurities through the micro-filtration membrane device, obtain micro-filtration membrane filtrating, the condition of work of micro-filtration membrane device is: pressure 0.2～0.25MPa, and 40～50 ℃ of temperature, flow is 90L/min, the micro-filtrate membrane filtration aperture is 0.1～1.0um;
(8) the micro-filtration membrane filtrating that obtains in the step (7) is concentrated through the NF membrane device again; Obtain the NF membrane concentrate, the condition of work of NF membrane device is: pressure 1.5～2MPa, 40～50 ℃ of temperature; Flow 20L/min, NF membrane pore size filter are 0.1～1.0nm;
(9) again that the NF membrane concentrate that obtains in the step (8) is dry, promptly obtain the powdery cottonseed protein.
2. a kind of method of from the cottonseed dregs of rice, extracting cottonseed protein according to claim 1; It is characterized in that: when extracting the bollworm organized enzyme in the step (3), the worm slurry is 1:5 with the weight ratio of amount of water, and the pH value is 5.0 ~ 6.0; Ultrasonic frequency is 30～40kHZ, and extraction time is 100s.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931871A (en) * | 2014-05-06 | 2014-07-23 | 襄阳汇源农林股份有限公司 | Method for preparing cottonseed meal albumen powder by adopting high-voltage corona electric field phenol reduction |
| CN103931872B (en) * | 2014-05-06 | 2016-01-20 | 襄阳汇源农林股份有限公司 | A kind of vinasse Cottonseed Meal soluble protein powder and preparation method |
| CN105733880A (en) * | 2016-04-06 | 2016-07-06 | 吉林省德盛农牧科技发展有限公司 | Composite hazelnut wine brewed from hazelnut meal |
| CN106106481A (en) * | 2016-06-16 | 2016-11-16 | 湖北工业大学 | A kind of bollworm NSLT inhibitor and virtual screening method thereof |
| CN106306329A (en) * | 2015-06-25 | 2017-01-11 | 南通海辰蛋白科技有限公司 | Extraction technology of cottonseed proteins |
| CN106801080A (en) * | 2017-01-22 | 2017-06-06 | 嵊州市派特普科技开发有限公司 | The method that cottonseed protein is extracted in Cottonseed Meal |
| WO2018226689A1 (en) * | 2017-06-07 | 2018-12-13 | Whitewave Services, Inc. | Systems and methods using physical energy technology to produce non-dairy protein base and value-added utilization of the co-product |
| CN109170266A (en) * | 2018-09-26 | 2019-01-11 | 梁耀培 | A method of vinasse powder and wheat ferment cream are produced using wheat vinasse |
| US11582987B2 (en) | 2017-06-07 | 2023-02-21 | Whitewave Services, Inc. | Systems and methods using physical energy technology to produce non-dairy protein base and value-added utilization of the co-product |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931871A (en) * | 2014-05-06 | 2014-07-23 | 襄阳汇源农林股份有限公司 | Method for preparing cottonseed meal albumen powder by adopting high-voltage corona electric field phenol reduction |
| CN103931871B (en) * | 2014-05-06 | 2015-11-25 | 襄阳汇源农林股份有限公司 | The preparation method of the Cottonseed Meal albumen powder of phenol falls in a kind of high-voltage corona electric field |
| CN103931872B (en) * | 2014-05-06 | 2016-01-20 | 襄阳汇源农林股份有限公司 | A kind of vinasse Cottonseed Meal soluble protein powder and preparation method |
| CN106306329A (en) * | 2015-06-25 | 2017-01-11 | 南通海辰蛋白科技有限公司 | Extraction technology of cottonseed proteins |
| CN105733880A (en) * | 2016-04-06 | 2016-07-06 | 吉林省德盛农牧科技发展有限公司 | Composite hazelnut wine brewed from hazelnut meal |
| CN106106481A (en) * | 2016-06-16 | 2016-11-16 | 湖北工业大学 | A kind of bollworm NSLT inhibitor and virtual screening method thereof |
| CN106106481B (en) * | 2016-06-16 | 2019-06-28 | 湖北工业大学 | A kind of bollworm sterol carrier protein 2 inhibitor and its virtual screening method |
| CN106801080A (en) * | 2017-01-22 | 2017-06-06 | 嵊州市派特普科技开发有限公司 | The method that cottonseed protein is extracted in Cottonseed Meal |
| WO2018226689A1 (en) * | 2017-06-07 | 2018-12-13 | Whitewave Services, Inc. | Systems and methods using physical energy technology to produce non-dairy protein base and value-added utilization of the co-product |
| RU2768395C2 (en) * | 2017-06-07 | 2022-03-24 | Уайтвейв Сервисиз, Инк. | Systems and methods using physical energy technology for producing a non-dairy protein base and recycling a by-product with added value |
| US11582987B2 (en) | 2017-06-07 | 2023-02-21 | Whitewave Services, Inc. | Systems and methods using physical energy technology to produce non-dairy protein base and value-added utilization of the co-product |
| CN109170266A (en) * | 2018-09-26 | 2019-01-11 | 梁耀培 | A method of vinasse powder and wheat ferment cream are produced using wheat vinasse |
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