CN102539327A - Integrating sphere device for dry-type chemical detection - Google Patents
Integrating sphere device for dry-type chemical detection Download PDFInfo
- Publication number
- CN102539327A CN102539327A CN2010105814436A CN201010581443A CN102539327A CN 102539327 A CN102539327 A CN 102539327A CN 2010105814436 A CN2010105814436 A CN 2010105814436A CN 201010581443 A CN201010581443 A CN 201010581443A CN 102539327 A CN102539327 A CN 102539327A
- Authority
- CN
- China
- Prior art keywords
- sample
- integrating sphere
- baffle
- detection
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses an integrating sphere device for dry-type chemical detection, and the device is characterized in that an integrating sphere is provided with a light-source entrance port and a sample detection inlet; the inside of the integrating sphere is provided with a first baffle and a second baffle, and the first baffle and the second baffle are arranged on the two sides of the light-source entrance port and located at a same longitude line with the light-source entrance port; a sample photoelectric detector is arranged on the first baffle and under the sample detection inlet, and the sample detecting surface of the sample photoelectric detector is dead against the sample detection inlet; and a reference photoelectric detector is arranged on the second baffle and under the sample photoelectric detector, and the reference detecting surface of the reference photoelectric detector is dead against the downward side. Compared with the prior art, the integrating sphere device disclosed by the invention can be used for reducing irrelevant optical background noises, and improving the accuracy and repeatability of detection results.
Description
Technical field
The present invention relates to a kind of optical devices, be specifically related to a kind of integrating sphere, in particular for the integrating sphere device of dry chemical detection.
Background technology
In clinical examination is analyzed, utilize blood, urine or other body fluid to carry out the clinical analysis check, can obtain the result of a plurality of clinical examination projects fast, be the doctor diagnose the most accurately, the most direct one of method.In carrying out the clinical examination analysis, the wet chemistry analytic approach that the most often adopts at present.It has used the method that is called as solution chemistry.Usually, adopting the instrument of wet chemistry analytic approach is that colourimetry with Lambert-Beer's law is the basis.This detection method need be passed through following steps: blood blood drawing, centrifugal, configuration reagent solution, Photoelectric Detection.The shortcoming of this type of detection method is: reagent needs to preserve with the liquid mode, need carry out preliminary work during detection, need carry out the centrifugal serum that obtains to whole blood, and whole detection needs the long time.
In order to overcome the shortcoming of wet chemistry analytic approach, people have worked out more simple and easy to do method, i.e. the dry chemical analytic approach.Solution is used in the not operational analysis of dry chemical analytic approach, and as reaction media, detected sample directly reacts with the dry chemical reagents of solidifying on solid phase carrier, and then detects with the liquid in the detected sample.It is different with the media that the difference of the maximum of conventional wet chemical analysis technology is to participate in chemical reaction, and wet-chemical analysis adopts solution as reaction media, and liquid in the testing sample is directly adopted in the dry chemical analysis, has therefore saved the link of configuration reagent solution.With respect to wet chemical analytical method, the dry chemical analytic approach has following advantage: detection is quick, operation is simple and easy, and sample can be whole blood, blood plasma or serum, and Litter is a solid dry chemical reagents sheet, and no waste liquid produces, environmental protection more.
Measuring system based on the dry chemical analytic approach comprises dry chemical analyser and dry chemical reagents sheet; The dry chemical analyser is used for for the dry chemical reagents sheet indispensable chemical reaction environment being provided; Like constant temperature system etc., and detect the reaction result of dry chemical reagents sheet automatically.General dry chemical analyser is based on two kinds of detection modes: differential potentiometry and reflection photometry.Differential potentiometry obtains testing result through the electric signal on the direct measurement dry plate, is commonly used in the situation of measuring inorganic ions etc.And the measurement of other type is adopted the reflection photometry when detecting colourimetry or rate method or immune rate method dry plate.
Reflection luminosity ratio juris is different according to reflecting medium, following several kinds of effects after light gets into medium, occur: 1) produce reflection and refraction at irradiating surface; 2) absorbed inside; 3) in inside or irradiating surface produce scattering; The summation of these effects has determined light in ratio that leaves medium and direction, and material is relevant on its final intensity of reflected light and the medium, can obtain the absorbance of dry chemical reagents sheet according to this principle, and then derive testing sample concentration.Therefore in dry chemical analyser, generally by forming: light supply apparatus, the monochromatic device of collimation, Electro-Optical Sensor Set, signal processing system and device displaying result with lower component based on the reflection photometry.
When utilizing the dry chemical analyser to detect; Through sending a branch of light,, certain chromogenic substrate is arranged in the dry chemical reagents sheet through shining on the dry chemical reagents sheet after monochromatic and the collimation at the instrument internal light source; Because this chromogenic substrate exists stronger specificity to absorb in the certain wave strong point; After carrying out chemical reaction, can absorb the light of this wavelength, through detecting the reflective light intensity behind the dry chemical reagents sheet chemical reaction, and then can obtain the concentration of testing sample with sample.When carrying out dry chemical method mensuration; Because determinand dry chemical reagents sheet is the analoids of multi-layer film structure normally; So detected light signal has comprised reflected light, refract light, scattered light and MIR light etc.; The existence of parasitic light causes the signal to noise ratio (S/N ratio) of Photoelectric Detection lower in the light signal, therefore causes the influence fluctuation that the testing result precision is lower, repeatability receives different determinands bigger.This makes present dry chemical analyser on accuracy and repeatability, compare gap in addition with the wet chemistry analyser.
For solving this type of problem; Patent (200410041858.9) has proposed the device that a kind of optical system of utilizing integrating sphere to cooperate the rear end is measured; Though this method has improved signal to noise ratio (S/N ratio) to a certain extent; But need carry out twice measurement respectively, and optical system more complicated to standard items and product to be tested.Other method comprises through increasing light intensity and improves signal to noise ratio (S/N ratio), increases enlargement factor and improves modes such as signal to noise ratio (S/N ratio) through utilizing, but owing to do not improve from photoelectric detection system, therefore can not fundamentally solve the problem of Photoelectric Detection.
Summary of the invention
In order to overcome the low deficiency bad of signal to noise ratio (S/N ratio) in the existing dry chemical analysis with repeatability; The objective of the invention is to; A kind of integrating sphere device of special construction is provided; This device is compared with existing photoelectric detection system, the optics ground unrest that can reduce to have nothing to do, the accuracy and the repeatability of raising testing result.
In order to solve the problems of the technologies described above, realize above-mentioned purpose, the present invention realizes through following technical scheme:
A kind of integrating sphere device that is used for the dry chemical detection is opened a light source entrance port and sample detection inlet on integrating sphere; Set inside at said integrating sphere has first baffle plate and second baffle, and said first baffle plate and second baffle are arranged in the both sides of said light source entrance port and are on the same warp with said light source entrance port; On said first baffle plate, sample detection inlet under be provided with the sample photodetector, the sample test surface of said sample photodetector faces said sample detection inlet; On the said second baffle, the sample photodetector under be provided with reference photodetector, the benchmark test surface of said reference photodetector is over against down.
Further, said light source entrance port and sample detection inlet angle between the two is 90 °.
Compared with prior art; The integrating sphere device that is used for the dry chemical detection of the present invention; Utilize the photodetector of two symmetric positions, improved the optical measurement especially signal to noise ratio (S/N ratio) and the accuracy of dry chemical measurement, but the while one-shot measurement goes out the light intensity and the benchmark light intensity of testing sample; Compare the process of having saved need replacing measurement standard plate benchmark light intensity with classic method, improved repeatability and detection speed.
Above-mentioned explanation only is the general introduction of technical scheme of the present invention, understands technological means of the present invention in order can more to know, and can implement according to the content of instructions, below with preferred embodiment of the present invention and conjunction with figs. specify as after.Embodiment of the present invention is provided by following examples and accompanying drawing thereof in detail.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.
Fig. 1 is the structural representation that is used for the integrating sphere device of dry chemical detection of the present invention.
Fig. 2 is the structural representation that is used for an embodiment of the integrating sphere device that dry chemical detects of the present invention.
Label declaration among the figure: 1, integrating sphere, 2, light source entrance port, 3, sample detection inlet, 41, first baffle plate, 42, second baffle; 51, sample photodetector, 511, the sample test surface, 52, reference photodetector; 521, benchmark test surface, 6, light source, 7, testing sample.
Embodiment
Referring to shown in Figure 1, a kind of integrating sphere device that is used for the dry chemical detection is opened a light source entrance port 2 and sample detection inlet 3 on integrating sphere 1; Set inside at said integrating sphere 1 has first baffle plate 41 and second baffle 42, and said first baffle plate 41 is arranged in the both sides of said light source entrance port 2 with second baffle 42 and is on the same warp with said light source entrance port 2; On said first baffle plate 41, sample detection inlet 3 under be provided with sample photodetector 51, the sample test surface 511 of said sample photodetector 51 faces said sample detection inlet 3; On the said second baffle 42, sample photodetector 51 under be provided with reference photodetector 52, the benchmark test surface 521 of said reference photodetector 52 is over against down.
Further, said light source entrance port 2 and sample detection inlet 3 angle between the two are 90 °.
Referring to shown in Figure 2; Enter the mouth after 3 places when testing sample 7 is put into sample detection, one be arranged at 2 places, light source entrance port light source 6 illumination of sending certain wavelength get into the inside of integrating sphere 1, light beam is integrated ball 1 white inner wall and repeatedly reflects; The rayed of homogenising is on testing sample 7; The light transmission testing sample 7 of homogenising is like the transparent support layer of dry chemical reagents sheet, after reagent layer is partially absorbed by colored compound, reflexes to once more in the integrating sphere 1 at the reflecting surface of diffusion layer.The light intensity that is positioned at integrating sphere 1 the first half has comparatively significantly different with the light intensity of the latter half; Its 51 light intensity that can receive here of the sample photodetection at testing sample 7 right opposite places; Can obtain the light intensity Rt of testing sample 7, be positioned at the light intensity R of reference photodetector 52 acceptance criterias of the relative Lower Half office of integrating sphere 1 simultaneously
0Electric signal after two photodetectors are changed is transferred into the back end signal processing module, is handled by microprocessor and calculates, and can get testing concentration value to the end.
Through its 51 the light intensity R of sample photodetection that obtains
tLight intensity R with reference photodetector 52
0, according to the Williams-Clapper formula
D
r＝lgR
0/(R
t-R
r)
In the formula, D
rBe reflection density (reflective density).R
0Be the intensity of reflected light of on-gauge plate (like the integrating sphere inwall), R
tBe the intensity of reflected light of testing sample, R
rBe the gamma correction factor.Can calculate reflection density D
r, further can calculate the concentration of testing sample.
Preferably; The integrating sphere device that is used for the dry chemical detection of the application of the invention is tested, and light source is selected the LED of 580nm, detects the dry chemical reagents sheet of corresponding GLU type; The accuracy of testing result the target value ± 5% in; The coefficient of variation CV of precision＜3% is higher than common testing result, has improved signal to noise ratio (S/N ratio) and accuracy.
Preferably; The integrating sphere device that is used for the dry chemical detection of the application of the invention is tested, and light source is selected the LED of 380nm, detects the dry chemical reagents sheet of corresponding ALT type; The accuracy of testing result the target value ± 8% in; The coefficient of variation CV of precision＜3% is higher than common testing result, has improved signal to noise ratio (S/N ratio) and accuracy.
The foregoing description just is to let the one of ordinary skilled in the art can understand content of the present invention and enforcement according to this in order technical conceive of the present invention and characteristics to be described, to be its objective is, can not limit protection scope of the present invention with this.The variation or the modification of every equivalence that the essence of content has been done according to the present invention all should be encompassed in protection scope of the present invention.
Claims (2)
1. one kind is used for the integrating sphere device that dry chemical detects, and it is characterized in that: on integrating sphere (1), open a light source entrance port (2) and sample detection inlet (3); Set inside at said integrating sphere (1) has first baffle plate (41) and second baffle (42), and said first baffle plate (41) and second baffle (42) are arranged in the both sides of said light source entrance port (2) and are on the same warp with said light source entrance port (2); Be provided with sample photodetector (51) under said first baffle plate (41) is gone up, sample detection enters the mouth (3), the sample test surface (511) of said sample photodetector (51) faces said sample detection inlet (3); Said second baffle (42) is gone up, sample photodetector (51) under be provided with reference photodetector (52), the benchmark test surface (521) of said reference photodetector (52) is over against down.
2. the integrating sphere device that is used for the dry chemical detection according to claim 1 is characterized in that: said light source entrance port (2) and sample detection inlet (3) angle between the two are 90 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105814436A CN102539327A (en) | 2010-12-09 | 2010-12-09 | Integrating sphere device for dry-type chemical detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105814436A CN102539327A (en) | 2010-12-09 | 2010-12-09 | Integrating sphere device for dry-type chemical detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102539327A true CN102539327A (en) | 2012-07-04 |
Family
ID=46346760
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105814436A Pending CN102539327A (en) | 2010-12-09 | 2010-12-09 | Integrating sphere device for dry-type chemical detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102539327A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109725164A (en) * | 2019-01-22 | 2019-05-07 | 杭州联晟生物科技有限公司 | Dry-type biochemical analysis system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106696A (en) * | 1985-09-30 | 1987-04-01 | 株式会社岛津制作所 | Integral ball measuring device in the spectrophotometer |
| JPH07116530A (en) * | 1993-10-25 | 1995-05-09 | Satake Eng Co Ltd | Automatic adjustment method and device for grain polishing degree |
| JPH11108824A (en) * | 1997-10-08 | 1999-04-23 | Kdk Corp | Photometer and analyser equipped therewith |
| JP2003004628A (en) * | 2001-06-18 | 2003-01-08 | Shiseido Co Ltd | Cell and method for spectroscopic analysis |
| CN201903498U (en) * | 2010-12-09 | 2011-07-20 | 苏州生物医学工程技术研究所 | Integrating sphere for dry type chemical detection |
-
2010
- 2010-12-09 CN CN2010105814436A patent/CN102539327A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106696A (en) * | 1985-09-30 | 1987-04-01 | 株式会社岛津制作所 | Integral ball measuring device in the spectrophotometer |
| JPH07116530A (en) * | 1993-10-25 | 1995-05-09 | Satake Eng Co Ltd | Automatic adjustment method and device for grain polishing degree |
| JPH11108824A (en) * | 1997-10-08 | 1999-04-23 | Kdk Corp | Photometer and analyser equipped therewith |
| JP2003004628A (en) * | 2001-06-18 | 2003-01-08 | Shiseido Co Ltd | Cell and method for spectroscopic analysis |
| CN201903498U (en) * | 2010-12-09 | 2011-07-20 | 苏州生物医学工程技术研究所 | Integrating sphere for dry type chemical detection |
Non-Patent Citations (3)
| Title |
|---|
| 《实用医技杂志》 19950430 葛亚南等 GT-4320型血液葡萄糖测定仪应用评价 第2卷, 第4期 * |
| 俞善丁: "《临床基础检验学》", 31 October 1997, article "干化学尿液分析仪的原理和特点" * |
| 葛亚南等: "GT—4320型血液葡萄糖测定仪应用评价", 《实用医技杂志》, vol. 2, no. 4, 30 April 1995 (1995-04-30) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109725164A (en) * | 2019-01-22 | 2019-05-07 | 杭州联晟生物科技有限公司 | Dry-type biochemical analysis system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8462340B2 (en) | Gel particle measuring apparatus | |
| US7271883B2 (en) | Refractive index sensor using internally reflective light beams | |
| JP2916637B2 (en) | Measuring device for diffuse spectral reflectance | |
| US20110188030A1 (en) | Microelectronic sensor device for optical examinations in a sample medium | |
| CN103336130A (en) | Whole-blood immunoassay device and blood analyzer with same | |
| TW200533908A (en) | A handheld device with a disposable element for chemical analysis of multiple analytes | |
| JPH10153597A (en) | Method for determining existence of serum variable | |
| KR20150031007A (en) | Diagnostic strip insertion type reading device | |
| US10126229B2 (en) | Optical measurement device | |
| JPS5925460B2 (en) | Nephelometric immunoassay method and device | |
| CN201051102Y (en) | Quick tester for gold colloid | |
| CN109863386A (en) | It is measured using the gross protein of whole blood refraction measurement | |
| JPH11511557A (en) | Method and apparatus for characterizing a specimen under ambient light | |
| JP6073317B2 (en) | Optical device for assay execution | |
| CN102564954A (en) | Multi-channel photoelectric detection device for dry type chemical analysis | |
| CN106680186A (en) | Multi-type scattered light detection system of flow cytometer | |
| CN201897567U (en) | Multichannel photoelectric detection device for dry type chemical analysis | |
| EP2614359A1 (en) | Device for reading assay results on test carrier | |
| CN201903498U (en) | Integrating sphere for dry type chemical detection | |
| CN108120678A (en) | A kind of detection device and method quantitative based on scattering light photothermal interference biochemistry | |
| US20070122914A1 (en) | Obtaining measurements of light transmitted through an assay test strip | |
| CN102539327A (en) | Integrating sphere device for dry-type chemical detection | |
| CN214011052U (en) | Integrated detection device for turbidimetry and scattering turbidimetry and protein analyzer | |
| JP2000356582A (en) | Optical measuring apparatus for measuring transmitted light and scattered light | |
| EP0110262A2 (en) | Optical readhead |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120704 |