CN102527658A - Pressure balance system of automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and automatic ELISA plate washer - Google Patents
Pressure balance system of automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and automatic ELISA plate washer Download PDFInfo
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- CN102527658A CN102527658A CN2012100138239A CN201210013823A CN102527658A CN 102527658 A CN102527658 A CN 102527658A CN 2012100138239 A CN2012100138239 A CN 2012100138239A CN 201210013823 A CN201210013823 A CN 201210013823A CN 102527658 A CN102527658 A CN 102527658A
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Abstract
The invention discloses a pressure balance system of an automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and the ELISA plate washer. The ELISA plate washer comprises a cleaning head, a vacuum pump and a plurality of lotion bottles for providing lotion for a lotion needle of the cleaning head; and the pressure balance system comprises a first multi-path joint, a main exhaust pipe and at least one auxiliary exhaust pipe, wherein the first multi-path joint is connected with the vacuum pump and the lotion bottles and used for balancing the pressure suffered by the lotion bottles, the main exhaust pipe and the at least one auxiliary exhaust pipe are respectively connected with the first multi-path joint and used for discharging air outside, and a switch is arranged on the auxiliary exhaust pipe and connected with a control circuit for controlling the on and off of the switch. According to the invention, the pressure balance system is arranged in the ELISA plate washer, so that the pressure of a pipeline in the ELISA plate washer can be adjusted automatically, the pressure generated by the vacuum pump can be matched with the flow of the lotion needle on the cleaning head automatically, the flowing rate of the lotion distributed for the lotion needle by the cleaning head can be kept consistent under any state, so that when a large plate washer is used for few pore plates, the damage of an instrument as part of washing channel is closed and excessive overflowing liquid overflows a tray and enters the machine can be prevented.
Description
Technical field
The present invention relates to the ELIAS microplate washer technical field, relate in particular to a kind of automatic ELIAS microplate washer pressure balancing system and automatic ELIAS microplate washer.
Background technology
(enzyme linked immunosorbent assay when ELISA) testing, uses automatic ELIAS microplate washer (hereinafter to be referred as washing the plate machine) to come to have neither part nor lot in the cleaning of enzyme mark orifice plate sample and the reagent of reaction usually at enzyme-linked immunosorbent assay.Washing the plate machine is made up of parts such as power supply, control circuit, compression pump (vavuum pump), pipeline, magnetic valve, mechanical system, cleaning head, carrier plate, pallet, bottle for handling liquid toilet or cosmetic substance, waste liquid bottles usually; Wherein, Compression pump is responsible in bottle for handling liquid toilet or cosmetic substance, producing malleation; In waste liquid bottle, producing negative pressure, is the power of liquid flow in the ELIAS microplate washer.
The cleaning head of washing the plate machine can be divided into 8 holes, 12 holes, 16 holes, 24 holes and 96 holes; The existing plate machine of washing is divided into usually and washes the plate machine for a short time and wash the plate machine greatly; For a short time wash the plate machine and can mate 8 holes, 12 holes, 16 holes and 24 hole cleaning heads, wash the plate machine greatly and can mate 96 hole cleaning heads.
For a short time wash the plate machine because piping flow changes not too greatly, when marking orifice plate, can not have the loine pressure problem with the enzyme of its flushing varying number lath.For washing the plate machine greatly, generally have 8 irrigation channels at least, when using simultaneously in 96 holes of cleaning head, because piping flow is bigger, therefore must the bigger compression pump of configuration.But, when the orifice plate of flushing negligible amounts, need close some irrigation channels usually, prevent that too much discharge from overflowing pallet, get into machine intimate, cause instrument damage.Owing to need close some irrigation channels on the 96 hole cleaning heads; Make the actual flow on the cleaning head reduce; The bigger compression pump that is disposed then possibly cause in the pipeline malleation to increase, if the flow of pressure and cleaning head cooperate processing bad, then can cause the washing lotion flow velocity too fast; The washing lotion flow regime is become by original laminar flow breathes heavily stream; Final generation washing lotion is overflowed or the orifice plate flushing is clean, the excessive phenomenon of debris, makes washing lotion overflow enzyme mark orifice plate, generation cross pollution or instrument damage when serious.
Summary of the invention
Main purpose of the present invention provides a kind of automatic ELIAS microplate washer pressure balancing system and automatic ELIAS microplate washer; Be intended to realize the automatic adjusting of the loine pressure in the ELIAS microplate washer, the flow of washing lotion pin on pressure that vavuum pump produces and the cleaning head is mated automatically.
In order to achieve the above object; The present invention proposes a kind of automatic ELIAS microplate washer pressure balancing system; Said ELIAS microplate washer comprises: cleaning head, vavuum pump and some bottle for handling liquid toilet or cosmetic substance that washing lotion is provided for the washing lotion pin of said cleaning head; Said pressure balancing system comprises: connect said vavuum pump and each bottle for handling liquid toilet or cosmetic substance, be used for the first multi-path joint of each bottle for handling liquid toilet or cosmetic substance pressure of balance; And the main exhaust and at least one auxilliary blast pipe that all are connected, are used for outside exhaust with the said first multi-path joint, said auxilliary blast pipe is provided with switch, and said switch is connected with the control circuit that is used to control said switch on and off.
Preferably, said system also comprises: muffler, and said muffler is connected through the exhaust outlet of sound-absorptive connector with said main exhaust and auxilliary blast pipe, and said muffler is provided with the outlet that is used for exhaust.
Preferably, the exhaust ports of said main exhaust is provided with the first adjusting vent valve that is used to regulate pressure at expulsion.
Preferably, the said first multi-path joint is provided with the second adjusting vent valve that is used to regulate pressure at expulsion.
Preferably, said switch is first magnetic valve, and said first magnetic valve is connected with said control circuit.
Preferably; Said ELIAS microplate washer also comprises the second multi-path joint; The said second multi-path joint connects each said bottle for handling liquid toilet or cosmetic substance and said cleaning head, is provided with some irrigation channels between said second multi-path joint and the said cleaning head, and each said irrigation channel correspondence is connected with some washing lotion pins.
Preferably, said irrigation channel is provided with and is used to control second magnetic valve that said irrigation channel turn-offs, and said second magnetic valve is connected with said control circuit.
Preferably, be provided with the 3rd magnetic valve that is used to control said bottle for handling liquid toilet or cosmetic substance work between said second multi-path joint and the said bottle for handling liquid toilet or cosmetic substance, said the 3rd magnetic valve is connected with said control circuit.
Preferably, said vavuum pump also is connected with waste liquid bottle, for said waste liquid bottle provides negative pressure; Said waste liquid bottle is connected with the aspirating needle of said cleaning head.
The present invention also proposes a kind of automatic ELIAS microplate washer, comprising: cleaning head, vavuum pump and for the washing lotion pin of said cleaning head provides some bottle for handling liquid toilet or cosmetic substance of washing lotion, it is characterized in that, and also comprise aforesaid automatic ELIAS microplate washer pressure balancing system.
A kind of automatic ELIAS microplate washer pressure balancing system and automatic ELIAS microplate washer that the present invention proposes; Through pressure balancing system is set in ELIAS microplate washer, can regulate the loine pressure in the Enzyme-linked washing-board machine automatically, the flow of washing lotion pin on pressure that vavuum pump produces and the cleaning head is mated automatically; The washing lotion flow velocity that cleaning head distributes for the washing lotion pin under any state keeps basically identical; Be laminar condition, thereby when preventing that the more plate machine of washing greatly of hole count from cleaning than oligoporous plate, overflow pallet because of the closed portion irrigation channel makes too much discharge; Get into machine intimate, cause instrument damage.
Description of drawings
Fig. 1 is the structural representation of the automatic ELIAS microplate washer of the present invention;
Fig. 2 is a cleaning head lotion dispenser sketch map in the automatic ELIAS microplate washer of the present invention;
Fig. 3 is the structural representation of the automatic ELIAS microplate washer pressure balancing system of the present invention;
Fig. 4 is the pressure distribution principle schematic of the automatic ELIAS microplate washer pressure balancing system of the present invention intermediate pump.
The realization of the object of the invention, functional characteristics and advantage will combine embodiment, further specify with reference to accompanying drawing.
The specific embodiment
The main solution of the embodiment of the invention is: through pressure balancing system is set in ELIAS microplate washer; Can regulate the loine pressure in the Enzyme-linked washing-board machine automatically; The flow of washing lotion pin on pressure that vavuum pump produces and the cleaning head is mated automatically, when preventing the more plate machine of washing the greatly cleaning of hole count, overflow pallet because of the closed portion irrigation channel makes too much discharge than oligoporous plate; Get into machine intimate, cause instrument damage.
The embodiment of the invention is that example describes with the plate machine of washing greatly with 96 hole cleaning heads, but is not limited thereto kind of a situation.
For make wash the plate machine can operate as normal, the coupling of washing the flow of washing lotion pin on pressure that plate machine intermediate pump produces and the cleaning head is extremely important, the plate machine is washed when designing in 96 holes; In order to make its lath that can clean varying number, the irrigation channel with cleaning head is divided into 8 the road or 12 the tunnel usually, when washing the plate machine with 96 holes and go to clean less orifice plate; Can close the irrigation channel of blank lath; Prevent that too much discharge from overflowing pallet, get into machine intimate, cause instrument damage.
In order to make washing the plate machine and can normally using of 96 hole cleaning heads, it is bigger to wash the compression pump that the plate machine uses, and after closing several roads irrigation channel, the pressure in the pipeline can increase a lot, and flow velocity is excessive in the time of might causing cleaning head distribution washing lotion.Excessive for fear of pipeline pressure, the present invention sets up a pressure balancing system in pipe-line system, when pressure increases; This system opens automatically; With the equalizing line internal pressure, similar when loine pressure state and 96 holes are opened simultaneously, thus make the washing lotion flow velocity of distribution consistent.
Particularly, please with reference to Fig. 1 and shown in Figure 4, Fig. 1 is the structural representation of the automatic ELIAS microplate washer of the present invention; Fig. 4 is the pressure distribution principle schematic of ELIAS microplate washer intermediate pump.This ELIAS microplate washer comprises: pallet 1, carrier plate 3, enzyme mark orifice plate 4, cleaning head 5, vavuum pump 2, waste liquid bottle 20, some bottle for handling liquid toilet or cosmetic substance (not shown among Fig. 1, please with reference to Fig. 2) and the control circuit etc. of washing lotion are provided for the washing lotion pin of cleaning head 5.
When cleaning; Aspirating needle and washing lotion move up and down to the holes in the accurate enzyme mark orifice plate 4, and the washing lotion pin is used for cleaning of enzyme mark orifice plate 4, and aspirating needle is used for siphoning away the washing lotion in enzyme mark orifice plate 4 holes; And the washing lotion that siphons away drained into waste liquid bottle 20, reach the purpose that enzyme mark orifice plate 4 is cleaned.
The effect of pallet 1 is except can supporting carrier plate 3; The washing lotion of overflowing and passing carrier plate 3 from enzyme mark orifice plate 4 when also being used for collecting cleaning head 5 cleaning of enzyme mark orifice plate 4; To wash the plate machine inner to prevent to flow to from the liquid that enzyme mark orifice plate 4 overflows, and causes and wash the short circuit of plate machine circuit or corrosion instrument base plate and parts etc.
Please with reference to Fig. 2, Fig. 2 is cleaning head 5 lotion dispenser sketch mapes in the automatic ELIAS microplate washer of the present invention.Be connected with the second multi-path joint 18 between each bottle for handling liquid toilet or cosmetic substance (among Fig. 2 with A bottle, B bottle, C bottle, D bottle example) and the cleaning head 5; Be provided with some irrigation channels 19 between the second multi-path joint 18 and the cleaning head 5; Each irrigation channel 19 correspondence is connected with some washing lotion pins 51, and aspirating needle 52 is spaced up and down with washing lotion pin 51.
For each irrigation channel 19 is controlled, on irrigation channel 19, be provided with magnetic valve (being alleged second magnetic valve 8 of present embodiment), second magnetic valve 8 is connected with control circuit.Conducting through 8 pairs of irrigation channels 19 of second magnetic valve is controlled with closing, and when the negligible amounts of the enzyme mark orifice plate 4 that cleans, in the time of need closing some irrigation channel 19, closes second magnetic valve 8 by control circuit control and makes irrigation channel 19 not conductings.
In addition, cleaning head 5 is furnished with several bottle for handling liquid toilet or cosmetic substance, and bottle for handling liquid toilet or cosmetic substance is normally worked successively; After the washing lotion in the bottle for handling liquid toilet or cosmetic substance used up, (or manual) switched to next bottle for handling liquid toilet or cosmetic substance (initial bottle for handling liquid toilet or cosmetic substance can be set) automatically, certainly; Can control wherein certain bottle for handling liquid toilet or cosmetic substance work, and other bottle for handling liquid toilet or cosmetic substance are not worked, in order to control bottle for handling liquid toilet or cosmetic substance work; Between the second multi-path joint 18 and bottle for handling liquid toilet or cosmetic substance, being provided with the 3rd magnetic valve 21, the three magnetic valves 21 is connected with control circuit.Wherein, in the ELIAS microplate washer pipeline flow direction of washing lotion shown in arrow among Fig. 2.
Washing the plate machine with 96 holes is example, when cleaning less enzyme mark orifice plate 4, can close several roads irrigation channel 19, prevents that too much discharge from overflowing pallet 1, gets into machine intimate, causes instrument damage.
After closing several roads irrigation channel 19, the pressure in the pipeline can increase a lot, and flow velocity is excessive in the time of might causing cleaning head 5 to distribute washing lotion.Excessive for fear of pipeline pressure, the present invention sets up a pressure balancing system in pipe-line system.
Please with reference to Fig. 3, Fig. 3 is the structural representation of the automatic ELIAS microplate washer pressure balancing system of the present invention; This pressure balancing system comprises: the first multi-path joint 10, main exhaust 11, at least one auxilliary blast pipe 12 and above-mentioned control circuit, wherein:
The first multi-path joint 10 is used for connecting vavuum pump 2 and each bottle for handling liquid toilet or cosmetic substance 9 (Fig. 3 is with A bottle, B bottle, C bottle, D bottle example), the malleation that balance vavuum pump 2 is provided for each bottle for handling liquid toilet or cosmetic substance 9, and the first multi-path joint 10 is connected with vavuum pump 2 through air inlet pipe 22.
Wherein, the quantity of above-mentioned auxilliary blast pipe 12 can be set according to the quantity of bottle for handling liquid toilet or cosmetic substance 9, the quantity and the real work situation of irrigation channel 19.
In order to control the open and close of auxilliary blast pipe 12, present embodiment is provided with switch on auxilliary blast pipe 12, and this switch can adopt magnetic valve (being alleged first magnetic valve 13 of present embodiment) or other valves, and present embodiment is that example describes with the magnetic valve.This first magnetic valve 13 is connected with control circuit, the conducting of controlling first magnetic valve 13 through control circuit with close and then the conducting and the shutoff of the auxilliary blast pipe 12 that is connected with first magnetic valve 13 of control.Like this; After ELIAS microplate washer was closed several roads irrigation channel 19, pipeline inner pressure can increase, and pressure balancing system starts automatically; First magnetic valve 13 through on each auxilliary blast pipe 12 of control circuit control is opened; Make the pipeline conducting of corresponding auxilliary blast pipe 12, pressure excessive in the pipeline is then discharged outside the ELIAS microplate washer through corresponding auxilliary blast pipe 12 and main exhaust 11, has guaranteed that thus cleaning head 5 lotion dispenser situations are basic identical under various states.
In addition; In order to prevent that main exhaust 11 and auxilliary blast pipe 12 from producing exhaust noise when the exhaust; The present embodiment pressure balancing system also comprises: muffler 14; This muffler 14 is connected through the exhaust outlet of sound-absorptive connector 15 with main exhaust 11 and auxilliary blast pipe 12, simultaneously, on muffler 14, is provided with the outlet that is used for exhaust.
Further; For regulating the pressure at expulsion of pressure balancing system; Can also be provided for regulating first of pressure at expulsion in the exhaust ports of main exhaust 11 and regulate vent valve 16, perhaps, on the first multi-path joint 10, be provided for regulating second of pressure at expulsion and regulate vent valve 17.On the first multi-path joint 10 second adjusting vent valve 17 can be regulated in advance when washing plate machine product export and set, and the first adjusting vent valve 16 of the exhaust ports of main exhaust 11 can be regulated by user oneself according to actual needs.
Present embodiment is through setting up a pressure balancing system in the pipe-line system of ELIAS microplate washer, when the less lath of cleaning made that loine pressure increases, this system opened automatically; With the equalizing line internal pressure; Similar when loine pressure state and 96 holes are opened simultaneously, thus the washing lotion flow velocity that cleaning head 5 is distributed is consistent, keeps laminar condition; Can not produce the phenomenon that pressure is excessive, flow velocity is too fast, cleaning fluid is sprayed and spoiled over out outward; Prevent that simultaneously too much discharge from overflowing pallet 1, get into machine intimate, cause instrument damage.
The above is merely the preferred embodiments of the present invention; Be not so limit claim of the present invention; Every equivalent structure or equivalent flow process conversion that utilizes specification of the present invention and accompanying drawing content to be done; Or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (10)
1. automatic ELIAS microplate washer pressure balancing system; Said ELIAS microplate washer comprises: cleaning head, vavuum pump and some bottle for handling liquid toilet or cosmetic substance that washing lotion is provided for the washing lotion pin of said cleaning head; It is characterized in that; Said pressure balancing system comprises: connect said vavuum pump and each bottle for handling liquid toilet or cosmetic substance, be used for the first multi-path joint of each bottle for handling liquid toilet or cosmetic substance pressure of balance; And the main exhaust and at least one auxilliary blast pipe that all are connected, are used for outside exhaust with the said first multi-path joint, said auxilliary blast pipe is provided with switch, and said switch is connected with the control circuit that is used to control said switch on and off.
2. system according to claim 1 is characterized in that, said system also comprises: muffler, and said muffler is connected through the exhaust outlet of sound-absorptive connector with said main exhaust and auxilliary blast pipe, and said muffler is provided with the outlet that is used for exhaust.
3. system according to claim 2 is characterized in that, the exhaust ports of said main exhaust is provided with the first adjusting vent valve that is used to regulate pressure at expulsion.
4. system according to claim 1 is characterized in that, the said first multi-path joint is provided with the second adjusting vent valve that is used to regulate pressure at expulsion.
5. system according to claim 1 is characterized in that, said switch is first magnetic valve, and said first magnetic valve is connected with said control circuit.
6. according to each described system among the claim 1-5; It is characterized in that; Said ELIAS microplate washer also comprises the second multi-path joint; The said second multi-path joint connects each said bottle for handling liquid toilet or cosmetic substance and said cleaning head, is provided with some irrigation channels between said second multi-path joint and the said cleaning head, and each said irrigation channel correspondence is connected with some washing lotion pins.
7. system according to claim 6 is characterized in that, said irrigation channel is provided with and is used to control second magnetic valve that said irrigation channel turn-offs, and said second magnetic valve is connected with said control circuit.
8. system according to claim 6 is characterized in that, is provided with the 3rd magnetic valve that is used to control said bottle for handling liquid toilet or cosmetic substance work between said second multi-path joint and the said bottle for handling liquid toilet or cosmetic substance, and said the 3rd magnetic valve is connected with said control circuit.
9. system according to claim 1 is characterized in that said vavuum pump also is connected with waste liquid bottle, for said waste liquid bottle provides negative pressure; Said waste liquid bottle is connected with the aspirating needle of said cleaning head.
10. automatic ELIAS microplate washer comprises: cleaning head, vavuum pump and for the washing lotion pin of said cleaning head provides some bottle for handling liquid toilet or cosmetic substance of washing lotion, it is characterized in that, and also comprise each described automatic ELIAS microplate washer pressure balancing system among the claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210013823.9A CN102527658B (en) | 2012-01-17 | 2012-01-17 | Pressure balance system of automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and automatic ELISA plate washer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210013823.9A CN102527658B (en) | 2012-01-17 | 2012-01-17 | Pressure balance system of automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and automatic ELISA plate washer |
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| Publication Number | Publication Date |
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| CN102527658A true CN102527658A (en) | 2012-07-04 |
| CN102527658B CN102527658B (en) | 2014-02-12 |
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| CN201210013823.9A Expired - Fee Related CN102527658B (en) | 2012-01-17 | 2012-01-17 | Pressure balance system of automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and automatic ELISA plate washer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102873062A (en) * | 2012-09-03 | 2013-01-16 | 深圳湃尔生物科技有限公司 | Cleaning method of elisa plate cleaning machine |
| CN103230897A (en) * | 2013-04-23 | 2013-08-07 | 上海裕隆生物科技有限公司 | Cleaning device and cleaning method |
| CN103817098A (en) * | 2014-01-27 | 2014-05-28 | 洛阳理工学院 | Cleaning and quick-dry device on basis of enzyme-linked reaction |
| CN105107784A (en) * | 2015-09-11 | 2015-12-02 | 港龙生物技术（深圳）有限公司 | High-throughput automatic cleaning system for microarray chip and using method |
| CN108543743A (en) * | 2018-05-03 | 2018-09-18 | 泰州泽成生物技术有限公司 | A kind of board-washing machine for realizing multichannel based on toggle switch |
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| CN2116217U (en) * | 1991-12-25 | 1992-09-16 | 陈吉坤 | Automatic enzyme sampler washer |
| US6761184B1 (en) * | 2003-11-25 | 2004-07-13 | Scott C. Jordan | Water flushing unit for a pressure balanced shower valve |
| CN101482560A (en) * | 2009-01-20 | 2009-07-15 | 邱重任 | ELIAS plate washer employing cleaning head and residual liquid suction nozzle with flow passage pressure balancing structure |
| CN201392341Y (en) * | 2009-01-14 | 2010-01-27 | 邱重任 | Cleaning head and residual liquid suction nozzle of enzyme label plate cleaning machine with flow passage pressure balance structure |
| CN202427672U (en) * | 2012-01-17 | 2012-09-12 | 深圳湃尔生物科技有限公司 | Automatic enzyme-labeling plate washer and pressure balance system for same |
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2012
- 2012-01-17 CN CN201210013823.9A patent/CN102527658B/en not_active Expired - Fee Related
Patent Citations (5)
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| CN2116217U (en) * | 1991-12-25 | 1992-09-16 | 陈吉坤 | Automatic enzyme sampler washer |
| US6761184B1 (en) * | 2003-11-25 | 2004-07-13 | Scott C. Jordan | Water flushing unit for a pressure balanced shower valve |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102873062A (en) * | 2012-09-03 | 2013-01-16 | 深圳湃尔生物科技有限公司 | Cleaning method of elisa plate cleaning machine |
| CN102873062B (en) * | 2012-09-03 | 2014-12-10 | 深圳湃尔生物科技有限公司 | Cleaning method of elisa plate cleaning machine |
| CN103230897A (en) * | 2013-04-23 | 2013-08-07 | 上海裕隆生物科技有限公司 | Cleaning device and cleaning method |
| CN103230897B (en) * | 2013-04-23 | 2016-01-20 | 上海裕隆生物科技有限公司 | A kind of cleaning device and cleaning method |
| CN103817098A (en) * | 2014-01-27 | 2014-05-28 | 洛阳理工学院 | Cleaning and quick-dry device on basis of enzyme-linked reaction |
| CN103817098B (en) * | 2014-01-27 | 2017-01-04 | 洛阳理工学院 | A kind of cleaning quick drying device based on integrated enzyme reaction |
| CN105107784A (en) * | 2015-09-11 | 2015-12-02 | 港龙生物技术（深圳）有限公司 | High-throughput automatic cleaning system for microarray chip and using method |
| CN108543743A (en) * | 2018-05-03 | 2018-09-18 | 泰州泽成生物技术有限公司 | A kind of board-washing machine for realizing multichannel based on toggle switch |
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| CN102527658B (en) | 2014-02-12 |
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Granted publication date: 20140212 Termination date: 20220117 |