CN102524037A - Method for acclimatizing desert algae from laboratory to desert environment - Google Patents

Method for acclimatizing desert algae from laboratory to desert environment Download PDF

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CN102524037A
CN102524037A CN2012100849654A CN201210084965A CN102524037A CN 102524037 A CN102524037 A CN 102524037A CN 2012100849654 A CN2012100849654 A CN 2012100849654A CN 201210084965 A CN201210084965 A CN 201210084965A CN 102524037 A CN102524037 A CN 102524037A
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algae
desert
soil
skinning
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张元明
张丙昌
王敬竹
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Xinjiang Institute of Ecology and Geography of CAS
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Xinjiang Institute of Ecology and Geography of CAS
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Abstract

The invention discloses a method for acclimatizing desert algae from a laboratory to the desert environment. The method comprises selecting six algae which are separated from desert algae crust and purified and include microcoleus vaginatus, phormidium, scytonema ocellatum, nostoc commune, synechococcus and chlorococcum humicola; building a shed at a place have the similarly weather conditions like the desert; selecting sand and paving the sand in the shed, and enabling the thickness of the sand to be 10cm; selecting the soil with the moisture to be 50%-80% and the temperature to be 10-15 DEG C, and performing inoculation at 10-20 DEG C; arranging 3 quadrats in the shed, and enabling a first quadrat not to be inoculated so as to serve as blank control; enabling two other quadrats to be inoculated, mixing algae powder of the 6 algae at a proportion of 16:1:1:1:1:1, enabling the inoculation amount to be 3g.m-2, using water to dilute the algae, and evenly spraying; and enabling the water using quantity each day to be 3L.m-2, and carrying out continuous exertion for 15 days. The method is simple, convenient and practical, successfully solves the problem of acclimatizing the desert algae from the laboratory to the desert environment, and has important practical value.

Description

The method that the desert algae is tamed to the desert environment from the laboratory
Invention field
The present invention relates to the biologic applications technical field of desert algae, specifically, the present invention relates to the desert algae when making up artificial algae skinning from indoor technical field of taming to the field.
Background technology
Desertization is one of main type of desertification, it be meant arid, semiarid and part half humid region since the man-land relationship inharmonious what cause is the land deterioration of outstanding feature with the wind-sand activity.China receives desertification to influence one of the most serious country (Wang Tao etc., 2005, Chinese desert, 25:816-817 in the world; It is originally strong to have mercy on etc., and 2009, aquatile journal, 33 (4): 756-761).The desertification land area reaches 264.42 ten thousand square kilometres, accounts for 27.5% of area, and annual direct economic loss reaches 54,000,000,000 yuan.Therefore, the reparation of damaged ecosystem and desertification treatment are the active demands that country improves the ecological environment, and the effect of biological breadcrust in desert ecosystem is more outstanding.Biological breadcrust is the organic complex that is formed by bryophyte, bacterium, fungi, blue-green alge, lichens and soil as the product of area, arid desert particular surroundings.Biological breadcrust has many functions for desert arid and semi-arid area: nitrogen fixation, for eremophytes and soil micro-ecosystem system provide nitrogen; Change the metal-chelating attitude into plant and can absorb form; Improve soil and gather ability, reduce wind erosion and sandslide; Make up a rough surface, thereby can catch moisture or nutrient content enriches particle.These functions have determined its important function in desert reparation and vegetation management.But the desert biological breadcrust very easily wrecks, in case destroyed, can quicken the desertization process.According to skinning size, complete skinning nature recovery time is not waited from several years to the more than ten years, and the cycle is very long.The ecologist is studying on the basis biological breadcrust both at home and abroad, constantly explores artificial culture and a large amount of breeding of biological breadcrust, carries out its application study, for defending and controlling sand of desertificated area provides new way low-cost, high benefit.
Realize cultivation and a large amount of breeding fast of biological breadcrust in the drought desert district, quicken the Application and Development of practical technique, the improvement of control of desert and ecotope is had important scientific meaning and realistic meaning.
Summary of the invention
Rare to precipitation; Evaporation discharge is big, utilizes the desert algae of artificial culture in the laboratory directly to inoculate into characteristics such as difficulty alive is big in the desert Environment in the open air, the method that the object of the present invention is to provide a kind of desert algae to tame to the desert environment from the laboratory; It is provided by the invention that method is simple; Workable, successfully solved the domestication problem of desert algae from indoor to the desert environment, for utilizing the desert algae successfully to make up artificial algae skinning a kind of feasible approach is provided.
The method that the present invention specifically provides the desert algae to tame to the desert environment from the laboratory, concrete grammar is following:
(1) selects the little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae for use, 6 kinds of algae kinds of separating of autochthonal Chlorococcum from the desert algae skinning; Condition of culture is that the little sheath algae of tool sheath is selected BG for use 11Medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2Lmin -1The eyespot Scytonema arcangelii is selected BG for use 110Medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2.5Lmin -1Nostoc commune is selected BG for use 110Medium, room temperature, intensity of illumination 40 μ Em -2S -1, aerobic culture, throughput are 1.5Lmin -1Collection ball algae and autochthonal Chlorococcum adopt BBM medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2Lmin -1
(2) select with the desert climate condition approachingly, promptly annual precipitation is at 70-150mm, the annual evaporation discharge more than 2000mm, 6-10 ℃ of year samming, high temperature is built booth in the place more than 40 ℃.
(3) the thinner sand of quality in the desert is selected after smooth in the soil in the booth, transports back, is tiled in the booth, and thickness 10cm gets final product.
(4) be chosen in soil moisture at 50%-80%, soil temperature is at 10-15 ℃, and temperature is inoculated under 10-20 ℃ of condition.
(5) in booth, establish 3 sample prescriptions, first sample prescription is not inoculated, as blank; The inoculation of two other sample prescription, with the little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae, the algae powder of autochthonal Chlorococcum is by weight being 16: 1: 1: after 1: 1: 1 the mixed, inoculum concentration is 3gm -2, algae kind dilute with water, sprayer evenly is sprayed in the sample prescription.
(6) 10 of every mornings are only executed water treatment to the sample prescription of one of them inoculation, and executing the water yield is 3Lm -2, apply 15 days continuously, in one month, can begin to take shape the algae skinning; There is not the formation of algae skinning and do not inoculate and inoculate the sample prescription that does not apply the moisture processing.
Among the present invention, every liter of BG 11Nutrient media components is following: NaNO 31.5g,, K 2HPO 40.04g, MgSO 47H 2O 0.07g, CaCl 22H 2O 0.036g, citric acid 0.006g, ferric citrate amine 0.006g, disodium edta 0.001g, CaCO 30.02g, micro-A 5Solution 1mL.Wherein, A 5Solution component: add H in every liter of redistilled water 3BO 32.86g, MnCl 24H 2O 1.86g, ZnSO 47H2O 0.22g, Na 2MoO 42H 2O 0.39g, CuSO 45H 2O 0.08g, Co (NO 3) 26H 2O 0.05g.
Every liter of BG 110Nutrient media components is following: MgSO 47H 2O 0.2g, CaCl 22H 2O 0.025g, K 2HPO 415g, H 3BO 30.6mg, ZnSO 47H 2O 0.04mg, MnCl 24H 2O 0.4mg, FeCl 36H 2O 2mg, Na 2MoO 40.4mg, Cu SO 45H 2O 0.04mg.
Every liter of BBM nutrient media components is following: NaNO 30.25g,, K 2HPO 40.075g, MgSO 47H 2O0.075g, CaCl 22H 2O 0.025g, KH 2PO 40.175g, NaCl 0.025g, trace element solution 1ml/L.Trace element solution component: add VB in every liter of redistilled water 11g, VB 60.5g, Na 2EDTA 0.05g, MnCl 24H 2O 0.0014g, FeSO 47H 2O 8.8g, MoO 30.7lg, CaSO 45H 2O 1.57g, Co (NO 3) 26H 2O 0.49g.
Among the present invention; The little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae; Autochthonal Chlorococcum is advantage species and common species in the desert biological breadcrust; Those of ordinary skills can separate acquisition from the desert natural environment, according to the technical scheme that the invention described above provides, can reproduce the method that desert algae provided by the invention is tamed to the desert environment from the laboratory.
Through the concrete technology contents of embodiment of the present invention, can reach following beneficial effect.
1. the nonvaccinated blank of the present invention is handled and is inoculated the processing sample prescription that does not apply moisture and do not have the formation of algae skinning; During inoculation is handled; In one month, can begin to take shape the algae skinning; Form in 3 months and stablize the algae skinning, its skinning algae bio amount (chlorophyll a), microbial biomass C, skinning thickness, compression strength, soil enzyme activities all are significantly higher than the nonvaccinated blank processing that does not apply moisture of handling and inoculate; The algae bio amount, microbial biomass carbon, skinning thickness and the compression strength that form the algae skinning after 3 months are respectively 5.19 ± 1.48mg chlorophyll a g -1Dry ground, 2.36 ± 0.39mg Cg -1Dry ground, 2.70 ± 0.46mm, 2.53 ± 0.31Ncm -2.
2. keep the stability of algal grown matrix, inoculum concentration is 3gm -2, executing the water yield is 3gL -2, execute the water time about 15 days, can begin to take shape the algae skinning.Success excessively provides a kind of otherwise effective technique scheme to the field from indoor to the invention provides the desert algae that utilizes laboratory cultures, to the desert check winds and fix drifting sand, the manual reversion of impaired desert ecosystem has important significance for theories and practice significance.
Description of drawings
The algae skinning figure that Fig. 1 formed for inoculation in one month, among the figure, a is not for having inoculation, no moisture; B is inoculation, anhydrous divisional processing; C is inoculation, has moisture to handle.
The algae skinning figure of Fig. 2 for forming in three months for inoculation, among the figure, a is not for having inoculation, no moisture; B is inoculation, anhydrous divisional processing; C is inoculation, has moisture to handle.
Fig. 3 relatively schemes A among the figure for chlorophyll, microbial biomass, skinning thickness and the compression strength of 3 months different disposal of inoculation: do not have inoculation+anhydrous divisional processing; B: inoculation+anhydrous divisional processing; C: inoculation+moisture is handled; Different lowercases are represented different disposal difference extremely significantly (P＜0.01).
Fig. 4 is the comparison diagram of soil enzyme activities in 3 months different disposal of inoculation, A among the figure: do not have inoculation+anhydrous divisional processing; B: inoculation+anhydrous divisional processing; C: inoculation+moisture is handled; Different lowercases are represented different disposal difference extremely significantly (P＜0.01).
Embodiment
Below, lift embodiment the present invention is described, still, the present invention is not limited to following embodiment.
All raw and auxiliary materials, reagent and the instrument of selecting for use among the present invention all is well known in the art, and other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention.
Embodiment one: the method that the desert algae is tamed to the desert environment from the laboratory
The method that the present invention specifically provides the desert algae to tame to the desert environment from the laboratory, its concrete grammar step is following:
(1) the algae kind is prepared: the present invention selects 6 kinds of algae kinds from the separation and purification of desert algae skinning for use: the little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae, autochthonal Chlorococcum; Condition of culture is that the little sheath algae of tool sheath is selected BG for use 11Medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2Lmin -1The eyespot Scytonema arcangelii is selected BG for use 110Medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2.5Lmin -1Nostoc commune is selected BG11 for use 0Medium, room temperature, intensity of illumination 40 μ Em -2S -1, aerobic culture, throughput are 1.5Lmin -1Collection ball algae and autochthonal Chlorococcum adopt BBM medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2Lmin -1
(2) point selection: select with the desert climate condition approaching, promptly in annual precipitation at 70-150mm, the annual evaporation discharge is more than 2000mm; 6-10 ℃ of year samming; High temperature is built booth in the place more than 40 ℃, and booth mainly is to be used for windproofly, keeps the stability of algal grown matrix.
(3) booth is handled in earlier stage: the thinner sand of quality in the desert is selected after smooth in the soil in the booth, transports back, is tiled in the booth, and the about 10cm of thickness gets final product.
(4) inoculation time is selected: be chosen in soil moisture at 50%-80%, soil temperature is at 10-15 ℃, and temperature is inoculated under 10-20 ℃ of condition, on moisture and temperature, all helps recovery and the growth of algae.
(5) with the little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae, the algae powder of autochthonal Chlorococcum was by 16: 1: 1: after 1: 1: 1 mixed, inoculum concentration is 3gm -2, algae kind dilute with water, sprayer evenly is sprayed in the sample prescription.
(6) every morning 10 water sprays, executing the water yield is 3Lm -2, apply 15 days continuously.In one month, can begin to take shape the algae skinning.
Among the present invention, every liter of BG 11Nutrient media components is following: NaNO 31.5g, K 2HPO 40.04g, MgSO 47H 2O 0.07g, CaCl 22H 2O 0.036g, citric acid 0.006g, ferric citrate amine 0.006g, disodium edta 0.001g, CaCO 30.02g, micro-A 5Solution 1mL.Wherein, A 5Solution component: add H in every liter of redistilled water 3BO 32.86g, MnCl 24H 2O 1.86g, ZnSO 47H2O 0.22g, Na 2MoO 42H 2O 0.39g, CuSO 45H 2O 0.08g, Co (NO 3) 26H 2O 0.05g.
Every liter of BG 110Nutrient media components is following: MgSO 47H 2O 0.2g, CaCl 22H 2O 0.025g, K 2HPO 415g, H 3BO 30.6mg, ZnSO 47H 2O 0.04mg, MnCl 24H 2O 0.4mg, FeCl 36H 2O 2mg, Na 2MoO 40.4mg, CuSO 45H 2O 0.04mg.
Every liter of BBM nutrient media components is following: NaNO 30.25g,, K 2HPO 40.075g, MgSO 47H 2O0.075g, CaCl 22H 2O 0.025g, KH 2PO 40.175g, NaCl 0.025g, trace element solution 1ml/L.Trace element solution component: add VB in every liter of redistilled water 11g, VB 60.5g, Na 2EDTA 0.05g, MnCl 24H 2O 0.0014g, FeSO 47H 2O 8.8g, MoO 30.71g, CaSO 45H 2O 1.57g, Co (NO 3) 26H 2O 0.49g.
Among the present invention; The little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae; Autochthonal Chlorococcum all is a common algae in the prior art; Those of ordinary skills can according to the technical scheme that the invention described above provides, can realize the method that desert algae provided by the invention is tamed to the desert environment from the laboratory through screening in outsourcing or the desert desert natural environment.
Embodiment two: the method effect assessment that the desert algae is tamed to the desert environment from the laboratory
This experiment is provided with the sample prescription of 3 2.5m * 2.5m in booth; Spread the drift sand that thickness is 10cm; Adopt different algae kinds to handle then respectively: A: do not inoculate (blank)+no water treatment; B: inoculation+no water treatment, several kinds of single algae kinds are mixed by a certain percentage, and the present invention preferentially adopts the little sheath algae of tool sheath: seat algae: eyespot Scytonema arcangelii: Nostoc commune: chlorella: autochthonal Chlorococcum=16: 1: 1: 1: 1: 1; C: inoculate+have water treatment, the algae kind is handled identical with B.
After 3 months, random sampling in sample prescription, algae bio amount, microbial biomass, skinning thickness, compression strength, soil enzyme activities (invertase, urase, alkaline phosphatase) are measured in 5 repetitions of each sample prescription sampling.Adopt in the soil chlorophyll a content to represent the algae bio amount, assay method referring to document (Xie et al., 2007, Soil Biology&Biochemistry, 567-572).Microbial biomass C adopts stifling-280nm ultraviolet colorimetric method, and concrete grammar is referring to document (Nunan et al., 1998, Soil Biology&Biochemistry, 30,1599-1603; Turner et al., 2001, Soil Biology&Biochemistry, 33,913-919).Adopt slide measure to measure the thickness of the algae skinning that forms, compression strength adopts compressive resistance meter (TE-3, Nanjing, China) to measure equal 5 repetitions of each sample prescription.The mensuration of soil enzyme activities is referring to document (Guan Songyin etc., 1986, soil enzyme and organon thereof; Zhou et al., 2012, Soil Biology&Biochemistry, 47,67-77).
Microbial biomass C is with fumigating-280nm ultraviolet colorimetric method: 30%～50%, 25 ℃ of lower seals that fresh soil specimen water content are adjusted to field moisture are cultivated 7～10d in advance, to keep all even gained result's of soil comparativity.The employing chloroform is stifling; The stifling soil sample of desire is placed on the internal diameter 29cm drier internal partition able to drawing air; Drier bottom is placed the 100ml beaker that 30ml does not have the ethanol chloroform is housed, and places 1 small beaker that distilled water is housed in addition, makes when stifling soil moisture content constant.Bleed thereupon, bubble boiling back occurs up to chloroform and continue 5min, stop to bleed; And then close drying device; And take out soil sample after being placed on room temperature (14～16 ℃) and the dark 24h of place, and place 2～3h in the place that ventilation is good, the chloroform in the residual soil is volatilized as far as possible.Not stifling soil sample places another drier, replaces chloroform with distilled water, and with stifling the same processings of chloroform, as stifling the contrast.Get stifling with not stifling soil sample, change in the 100ml triangular flask, added 0.5mol L-1K than 1: 4 by Tu Shui 2SO 4Solution filters behind the vibration 30min, under the 280nm ultraviolet light, measures absorbance immediately, the stifling and not stifling same treatment of doing.With the absorbance incremental representation in the unit soil, that is: δ/g oven-dried soil=(abs smoked/G smoked)-(abs not /G not), wherein: abs represent the absorbance the 280nm ultraviolet light under, and the oven-dried soil that the native heavy phase of G representative title is worked as weighs.Calculate microbial biomass C with Nunan et al. (1998) method.
Invertase: with 3,5-dinitrosalicylic acid colorimetric method is claimed the 5g air dried soil; Inject 15ml 8% sucrose solution, add 5ml pH5.5 buffer solution, cultivate 24h down for 37 ℃; Add 3; Behind the 5-dinitrosalicylic acid, the colorimetric estimation of 508nm place, the glucose numerical table that per hour generates with the 1g soil sample shows sucrase active.
Urase: use colorimetric method for determining, take by weighing the 5g air dried soil, add the buffer solution of 10ml 10% urea liquid and 20mlpH6.7, cultivate 24h down for 37 ℃, getting filtrating adding sodium phenate and liquor natrii hypochloritis, 578nm colorimetric estimation, the NH that per hour generates with the 1g soil sample 3The quantitaes urease activity of-N.
Alkaline phosphatase: use the disodium phenyl phosphate colorimetric method for determining; Get the 5g air dried soil and add the disodium phenyl phosphate solution of 5ml 27g L-1 and the buffer solution of 10ml pH10; Cultivate 3h down for 37 ℃, filter the back and add 2,6-dibromoquine-4-chlorimide solution; The colorimetric estimation of 600nm place, the phenol quantitaes phosphatase activity that per hour generates with the 1g soil sample.
In conjunction with referring to accompanying drawing 1,2,3 and 4, draw to draw a conclusion through above-mentioned experiment and detection validation:
Nonvaccinated space management does not have the formation of algae skinning with the processing sample prescription that inoculation does not apply moisture; In one month, can begin to take shape the algae skinning through what inoculate, form in 3 months and stablize the algae skinning.The algae bio amount, microbial biomass carbon, skinning thickness and the compression strength that form the algae skinning after 3 months are respectively 5.19 ± 1.48mg chlorophyll a g -1Dry ground, 2.36 ± 0.39mg Cg -1Dry ground, 2.70 ± 0.46mm, 2.53 ± 0.31Ncm -2The algae skinning that formed in 3 months, its skinning biomass, compression strength, soil enzyme activities all are significantly higher than the nonvaccinated blank processing that does not apply moisture of handling and inoculate; The stability that has kept algal grown matrix, inoculum concentration are 3gm -2, executing the water yield is 3Lm -2, execute the water time about 15 days, can form stable algae skinning.
Indicate that desert algae provided by the invention has the effect and the effect of realization from the laboratory to the method for desert environment domestication.

Claims (1)

1. the desert algae to the method for desert environment domestication, is characterized in that described method is following from the laboratory:
(1) selects the little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae for use, 6 kinds of algae kinds of separating of autochthonal Chlorococcum from the desert algae skinning; Condition of culture is that the little sheath algae of tool sheath is selected BG for use 11Medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2Lmin -1The eyespot Scytonema arcangelii is selected BG for use 110Medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2.5Lmin -1Nostoc commune is selected BG for use 110Medium, room temperature, intensity of illumination 40 μ Em -2S -1, aerobic culture, throughput are 1.5Lmin -1Collection ball algae and autochthonal Chlorococcum adopt BBM medium, room temperature, intensity of illumination 80 μ Em -2S -1, aerobic culture, throughput are 2Lmin -1
(2) select with the desert climate condition approachingly, promptly annual precipitation is at 70-150mm, the annual evaporation discharge more than 2000mm, 6-10 ℃ of year samming, high temperature is built booth in the place more than 40 ℃;
(3) the thinner sand of quality in the desert is selected after smooth in the soil in the booth, transports back, is tiled in the booth, and thickness 10cm gets final product;
(4) be chosen in soil moisture at 50%-80%, soil temperature is at 10-15 ℃, and temperature is inoculated under 10-20 ℃ of condition;
(5) in booth, establish 3 sample prescriptions, first sample prescription is not inoculated, as blank; The inoculation of two other sample prescription, with the little sheath algae of tool sheath, seat algae, eyespot Scytonema arcangelii, Nostoc commune, collection ball algae, the algae powder of autochthonal Chlorococcum was with weight ratio 16: 1: 1: after mixing at 1: 1: 1, inoculum concentration is 3gm -2, algae kind dilute with water, sprayer evenly is sprayed in the sample prescription;
(6) 10 of every mornings are only executed water treatment to the sample prescription of one of them inoculation, and executing the water yield is 3Lm -2, apply 15 days continuously, in one month, can begin to take shape the algae skinning; There is not the formation of algae skinning and do not inoculate and inoculate the sample prescription that does not apply the moisture processing;
(7) in the described medium of above-mentioned steps (1), every liter of BG 11Nutrient media components is following: NaNO 31.5g,, K 2HPO 40.04g, MgSO 47H 2O 0.07g, CaCl 22H 2O 0.036g, citric acid 0.006g, ferric citrate amine 0.006g, disodium edta 0.001g, CaCO 30.02g, micro-A 5Solution 1mL; Wherein, A 5Solution component: add H in every liter of redistilled water 3BO 32.86g, MnCl 24H 2O 1.86g, ZnSO 47H2O 0.22g, Na 2MoO 42H 2O 0.39g, CuSO 45H 2O 0.08g, Co (NO 3) 26H 2O0.05g;
Every liter of BG 110Nutrient media components is following: MgSO 47H 2O 0.2g, CaCl 22H 2O 0.025g, K 2HPO 415g, H 3BO 30.6mg, ZnSO 47H 2O 0.04mg, MnCl 24H 2O 0.4mg, FeCl 36H 2O 2mg, Na 2MoO 40.4mg, CuSO 45H 2O 0.04mg;
Every liter of BBM nutrient media components is following: NaNO 30.25g,, K 2HPO 40.075g, MgSO 47H 2O0.075g, CaCl 22H 2O 0.025g, KH 2PO 40.175g, NaCl 0.025g, trace element solution 1ml/L; Trace element solution component: add VB in every liter of redistilled water 11g, VB 60.5g, Na 2EDTA 0.05g, MnCl 24H 2O 0.0014g, FeSO 47H 2O 8.8g, MoO 30.71g, CaSO 45H 2O 1.57g, Co (NO 3) 26H 2O 0.49g.
CN2012100849654A 2012-03-28 2012-03-28 Method for acclimatizing desert algae from laboratory to desert environment Pending CN102524037A (en)

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CN107996054A (en) * 2017-11-13 2018-05-08 中国矿业大学 A kind of artificial algae skinning method suitable for arid and semi-arid area plant on sand land
CN111363683A (en) * 2019-04-17 2020-07-03 张兰英 Method and device for culturing edible tree fungi in indoor and outdoor artificial large-scale cultivation land
CN113846018A (en) * 2021-10-11 2021-12-28 新疆金正生物科技有限公司 Method for rapidly screening high-temperature-resistant desert algae
CN115069764A (en) * 2022-08-02 2022-09-20 内蒙古自治区林业科学研究院 Inoculation method of desert algae

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Cited By (6)

* Cited by examiner, † Cited by third party
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CN105052509A (en) * 2015-08-24 2015-11-18 中国科学院水生生物研究所 Inoculation method of desert alga artificial biological crusts for sand stabilization
CN107996054A (en) * 2017-11-13 2018-05-08 中国矿业大学 A kind of artificial algae skinning method suitable for arid and semi-arid area plant on sand land
CN111363683A (en) * 2019-04-17 2020-07-03 张兰英 Method and device for culturing edible tree fungi in indoor and outdoor artificial large-scale cultivation land
CN113846018A (en) * 2021-10-11 2021-12-28 新疆金正生物科技有限公司 Method for rapidly screening high-temperature-resistant desert algae
CN113846018B (en) * 2021-10-11 2023-08-15 新疆金正生物科技有限公司 Method for rapidly screening high-temperature-resistant desert algae seeds
CN115069764A (en) * 2022-08-02 2022-09-20 内蒙古自治区林业科学研究院 Inoculation method of desert algae

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