CN102521647B - Metering tank and manufacture method thereof - Google Patents
Metering tank and manufacture method thereof Download PDFInfo
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- CN102521647B CN102521647B CN201110386440.1A CN201110386440A CN102521647B CN 102521647 B CN102521647 B CN 102521647B CN 201110386440 A CN201110386440 A CN 201110386440A CN 102521647 B CN102521647 B CN 102521647B
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- flat board
- measurement plate
- injecting glue
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Abstract
The invention discloses a metering tank, which comprises a measuring plate and a surface plate, wherein the measuring plate and the surface plate are both made of transparent organic material; the measuring plate is provided with a plurality of measuring tanks; the periphery of each measuring tank is provided with a step; the plane height of the step is higher than the plane height of the measuring tank and the plane height of the measuring plate; the plane of the measuring tank is provided with a sample filling hole; and one side of the measuring plate, which is provided with the measuring tanks, is fixed with the surface plate together. In the scheme provided by the invention, the measuring plate and the surface plate are fixed together, and the measuring plate and the surface plate do not need to be installed before being used each time; and in addition, because the measuring plate and the surface plate are both made of organic materials, the construction cost is low. The metering tank provided by the invention can be replaced by a new metering tank after being used once, and therefore the trouble that the metering tank needs to be cleaned after the metering tank is used each time is avoided. Therefore, the metering tank provided by the invention is convenient to use and does not need to be cleaned in the experiment, the manpower cost is lowered, the measurement precision is guaranteed and the like.
Description
Technical field
The present invention relates to particulate identification and counting field, in particular, relate to measuring tank and preparation method thereof.
Background technology
Refer to shown in Fig. 1 and Fig. 2, blood counting chamber is a special thick microslide, has 4 grooves and form 3 platforms on microslide.Middle platform is wider, in the middle of it, is separated into two halves again by a hyphen groove, respectively has a square grid above each one side of something, wherein, as shown in Figure 3, each square grid is divided into 9 large lattice to square grid, large lattice in the middle of it are called as counting chamber, are often used as the counting of microorganism.Counting chamber base plane is than the low 0.1mm of both sides cover glass holding plane, and after covered, the height between cover glass and counting chamber bottom is 0.1mm.In counting chamber, there is graduated structure.Be that each large grid is made up of 400 lattices.Each large grid length of side is 1mm, and the area of each large grid is 1mm2, and the area of each lattice is 1/400mm2.
Use blood counting chamber counting to need 3 steps: step 1, assembling: get clean tally, then a covered; Step 2, observation counting: need working sample in the filling of the edge of cover glass, sample sucks counting chamber, then be placed into micro-Microscopic observation, the micro organism quantity in grid is selected in statistical counting chamber, then is converted into the quantity of microbial cell in every milliliter of bacterium liquid (or every gram of sample); Step 3, cleaning-drying: blood counting chamber is rinsed well with water column after using on water swivel, be sure not to scrub or smear wiping with hard thing, in order to avoid damage grid scale.After cleaning, dry voluntarily or hair-dryer dries up, or make it dry with 95% ethanol or absolute ethyl alcohol or acetone dehydration, then microscopy observes in counting chamber whether have residual thalline or other sediments, if not totally, must repeated washing until totally.
In the research and practice process to prior art, the present inventor finds that prior art exists following problem:
First, before using blood counting chamber, need to install, after use finishes, also need to disassemble, so its operating process relative complex of existing blood counting chamber; And, if install incorrect, also can affect measuring accuracy, thus to operating personnel require high.
Secondly, after using blood counting chamber, in order to guarantee that it is inner clean, so that reuse next time, need repeatedly careful cleaning, thereby it is high to cause testing cost of labor, waste of manpower; And, in cleaning process, if dealt with improperly, may cause blood counting chamber contaminated, thereby cannot guarantee the precision that measure next time.
Summary of the invention
In view of this, purpose of design of the present invention is, a kind of measuring tank is provided, with easy to use, and the human cost that avoids waste, and guaranteed measuring accuracy.
The embodiment of the present invention is achieved in that
A kind of measuring tank, comprises measurement plate and the flat board made by transparent organic material;
On described measurement plate, be provided with multiple measuring flumes, the surrounding of each measuring flume is provided with step, and the level of described step, higher than the level of described measuring flume and the level of described measurement plate, is provided with sample filler port in the plane of described measuring flume;
A side and described flat board that described measurement plate has measuring flume are fixed together.
Preferably, in above-mentioned measuring tank, on described flat board, be provided with multiple hole for injecting glue;
The hole for injecting glue of described flat board is between the step of two adjacent measuring flumes of described measurement plate.
Preferably, in above-mentioned measuring tank, described measurement plate has in the plane of measuring flume one side and is provided with reference column, is provided with the pilot hole suitable with described reference column on described transparent plate.
Preferably, in above-mentioned measuring tank, on described measurement plate, be provided with pilot hole, on described transparent plate, be provided with the reference column suitable with described pilot hole.
Preferably, in above-mentioned measuring tank, the quantity of described pilot hole and reference column is 2.
Preferably, in above-mentioned measuring tank, the side that described measurement plate has measuring flume is fixed together by glue and described flat board.
A method for making for measuring tank, comprising:
1), make measuring flume: on the measurement plate of making at transparent organic material, produce multiple measuring flumes;
2), make step: produce the step of height higher than the level of described measuring flume and the level of described measurement plate in the surrounding of each measuring flume;
3), punching: get filler port in the plane of described measuring flume;
4), laminating a: side and the flat board described measurement plate to measuring flume are fixed together, and apply uniform pressure on flat board, so that the plane of the step of described measurement plate and flat board fit tightly;
5), injecting glue: the gap location between described flat board and measurement plate, filling glue makes described flat board and measure plate to be fixed together.
Preferably, in the method for making of above-mentioned measuring tank, in step 4) before, also comprise step 6):
6), make hole for injecting glue: on the flat board of making at transparent organic material, produce multiple hole for injecting glue, the hole for injecting glue of described flat board is between the step of two adjacent measuring flumes of described measurement plate;
Described step 5) be specially:
5), injecting glue: gap location and hole for injecting glue place between described flat board and measurement plate, filling glue makes described flat board and measure plate to be fixed together.
Compared with prior art, the technical scheme that the present embodiment provides has the following advantages and feature:
In scheme provided by the invention, measure plate and flat board and be fixed together, before each use, do not need it to install; And, to measure plate and flat board and all adopt organic material to make, cost is lower, is once replaceable new so measuring tank provided by the invention uses, and can remove from like this and need the trouble of cleaning after each use.Therefore, measuring tank provided by the invention, in experiment, has easy to usely, without cleaning, reduces human cost, guarantees the advantage such as measuring accuracy.
Accompanying drawing explanation
In order to be illustrated more clearly in the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Shown in Fig. 1 is the front elevation of existing blood counting chamber;
Shown in Fig. 2 is the sectional view of Fig. 1;
Shown in Fig. 3 is the square grid on existing blood counting chamber;
Shown in Fig. 4 is the front elevation of measurement plate provided by the invention;
Shown in Fig. 5 is the schematic diagram of flat board provided by the invention;
Shown in Fig. 6 is the schematic diagram of measuring tank provided by the invention;
Shown in Fig. 7 is the sectional view of measurement plate provided by the invention;
Shown in Fig. 8 is a kind of process flow diagram of method for making of measuring tank;
Shown in Fig. 9 is the process flow diagram of the method for making of another kind of measuring tank.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on the embodiment in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Refer to shown in Fig. 4 to Fig. 7, shown in Fig. 4 to Fig. 7 is a kind of measuring tank, comprises the measurement plate 1 and dull and stereotyped 2 of making by transparent organic material; Wherein, on described measurement plate 1, be provided with multiple measuring flumes 11, the surrounding of each measuring flume 11 is provided with step 12, the level of described step 12, higher than the level of described measuring flume 11 and the level of described measurement plate 1, is provided with sample filler port 13 in the plane of described measuring flume 11; The side and described dull and stereotyped 2 that described measurement plate 1 has measuring flume 11 is fixed together.
In the embodiment shown in Fig. 4 to Fig. 7, a side and described dull and stereotyped 2 measurement plate 1 to measuring flume 11 adopts injecting glues to fix conventionally, between the step 12 of two measuring flumes 11 that measurement plate 1 is adjacent, is injecting glue plane.When injecting glue, need to push down uniformly and measure plate 1 and flat board 2, the step 12 of measuring plate 1 is contacted with dull and stereotyped 2, then, glue is injected into the gap location of measuring plate 1 and dull and stereotyped 2, so that measurement plate 1 and dull and stereotyped 2 is bonded together by glue.But, owing to cannot completely glue being injected in injecting glue plane by the gap of measuring between plate 1 and dull and stereotyped 2, thereby cause injecting glue inhomogeneous.In order to address this problem, can multiple hole for injecting glue 21 be set on described dull and stereotyped 2, wherein, described dull and stereotyped 2 hole for injecting glue 21 is between the step 12 of two adjacent measuring flumes 11 of described measurement plate 1.Staff is in the process of injecting glue, can carry out injecting glue with this two place, gap of measuring plate 1 to hole for injecting glue 21 and dull and stereotyped 2 simultaneously, glue just can be filled in whole injecting glue plane like this, thereby makes the effect of injecting glue more desirable, has guaranteed to measure the fastness between plate 1 and dull and stereotyped 2.
In the embodiment shown in Fig. 4 to Fig. 7; in injecting glue process; measure between plate 1 and dull and stereotyped 2 and there is no locating device, its two often can be because mobile and change their relative position, between plate 1 and dull and stereotyped 2, need the position of locating device guarantee its two can not be offset so measure.For this reason, can have in the plane of measuring flume one side reference column 14 is set measuring plate 1, and on dull and stereotyped 2, the pilot hole 22 suitable with reference column 14 is set.Like this, before injecting glue, can measurement plate 1 and dull and stereotyped 2 be fixed by reference column 14 and pilot hole 22, after fixing, carry out again injecting glue, so not only can guarantee that the position of measuring plate 1 and dull and stereotyped 2 can not be offset, and, can also avoid glue to enter in the measuring flume 11 of measuring plate 1.In addition, in order to separate the problem of decision bit, can also on measurement plate 1, pilot hole be set, and on dull and stereotyped 2, the reference column suitable with described pilot hole be set, equally can head it off.
In Fig. 4 to Fig. 7, the quantity of pilot hole and reference column is 2, and certainly, the quantity of pilot hole and reference column is not limited to 2, can adjust voluntarily according to actual needs.
Shown in Figure 8, shown in Fig. 8 is a kind of process flow diagram of method for making of measuring tank, comprising:
Step S11, making measuring flume: on the measurement plate of making at transparent organic material, produce multiple measuring flumes;
Wherein, the quantity of measuring flume can change according to the actual needs of work, does not limit at this.
Step S12, making step: produce the step of height higher than the level of described measuring flume and the level of described measurement plate in the surrounding of each measuring flume;
Wherein, measure in the step that the step of plate can guarantee injecting glue in the back, glue can not enter into measuring flume.
Step S13, punching: in the plane of described measuring flume, get filler port;
Wherein, filler port is the entrance that adds tracer liquid, for tracer liquid can be entered in measuring flume faster, can in the plane of measuring flume, get multiple filler ports.
To step S13, both can produce respectively measuring flume, step and filler port by three steps at step S11, also can directly measurement plate be produced to measuring flume, step and filler port by mould.
Step S14, laminating: a side and the flat board described measurement plate to measuring flume are fixed together, and apply uniform pressure on flat board, so that the plane of the step of described measurement plate and flat board fit tightly;
Wherein, to there is not gap in order guaranteeing to measure between the step of plate and flat board, to avoid glue to enter in measuring flume from measure the step of plate and dull and stereotyped gap, need to measure plate and dull and stereotyped bonded to each other after, apply uniform pressure.
Step S15, injecting glue: the gap location between described flat board and measurement plate, filling glue makes described flat board and measures plate to be fixed together;
Wherein, the gap location of measuring between plate and flat board is exactly injecting glue plane, in this injecting glue plane, injects glue, after glue solidifies, just can be fixed together measuring plate and flat board.
Shown in Figure 9, shown in Fig. 9 is the process flow diagram of the method for making of another kind of measuring tank, comprising:
Step S21, making measuring flume: on the measurement plate of making at transparent organic material, produce multiple measuring flumes;
Wherein, the quantity of measuring flume can change according to the actual needs of work, does not limit at this.
Step S22, making step: produce the step of height higher than the level of described measuring flume and the level of described measurement plate in the surrounding of each measuring flume;
Wherein, measure in the step that the step of plate can guarantee injecting glue in the back, glue can not enter into measuring flume.
Step S23, punching: in the plane of described measuring flume, get filler port;
Wherein, filler port is the entrance that adds tracer liquid, for tracer liquid can be entered in measuring flume faster, can in the plane of measuring flume, get multiple filler ports.
To step S23, both can produce respectively measuring flume, step and filler port by three steps at step S21, also can directly measurement plate be produced to measuring flume, step and filler port by mould.
Step S24, making hole for injecting glue: on the flat board of making at transparent organic material, produce multiple hole for injecting glue, the hole for injecting glue of described flat board is between the step of two adjacent measuring flumes of described measurement plate;
Wherein, after making hole for injecting glue, staff is in the time of the process of injecting glue, can carry out injecting glue to hole for injecting glue and this two place, gap dull and stereotyped and measurement plate simultaneously, glue just can be filled in whole injecting glue plane like this, thereby make the effect of injecting glue more desirable, guaranteed to measure the fastness between plate and flat board.
Step S25, laminating: a side and the flat board described measurement plate to measuring flume are fixed together, and apply uniform pressure on flat board, so that the plane of the step of described measurement plate and flat board fit tightly;
Wherein, to there is not gap in order guaranteeing to measure between the step of plate and flat board, to avoid glue to enter in measuring flume from measure the step of plate and dull and stereotyped gap, need to measure plate and dull and stereotyped bonded to each other after, apply uniform pressure.
Step S26, at described flat board with measure gap location and the hole for injecting glue place between plate, filling glue makes described flat board and measures plate to be fixed together.
Wherein, the gap location of measuring between plate and flat board is exactly injecting glue plane, in this injecting glue plane, injects glue, and, inject glue to hole for injecting glue place simultaneously, after solidifying, glue just can be fixed together measuring plate and flat board.
In sum, be fixed together owing to measuring plate and flat board, so in use, just do not need to have mounted and dismounted; And, all adopt organic material to make owing to measuring plate and flat board, cost is lower, is once replaceable new so measuring tank provided by the invention uses, and can remove from like this and need the trouble of cleaning after each use.Therefore, measuring tank provided by the invention, in experiment, has easy to usely, without cleaning, reduces human cost, guarantees the advantage such as measuring accuracy.
To be apparent for those skilled in the art to the multiple modification of these embodiment, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (6)
1. a measuring tank, is characterized in that, comprises measurement plate and the flat board made by transparent organic material;
On described measurement plate, be provided with multiple measuring flumes, the surrounding of each measuring flume is provided with step, and the level of described step, higher than the level of described measuring flume and the level of described measurement plate, is provided with sample filler port in the plane of described measuring flume;
A side and described flat board that described measurement plate has measuring flume are fixed together;
On described flat board, be provided with multiple hole for injecting glue;
The hole for injecting glue of described flat board is between the step of two adjacent measuring flumes of described measurement plate.
2. measuring tank according to claim 1, is characterized in that, described measurement plate has in the plane of measuring flume one side and is provided with reference column, is provided with the pilot hole suitable with described reference column on described transparent plate.
3. measuring tank according to claim 1, is characterized in that, on described measurement plate, is provided with pilot hole, is provided with the reference column suitable with described pilot hole on described transparent plate.
4. according to the measuring tank described in claim 2 or 3, it is characterized in that, the quantity of described pilot hole and reference column is 2.
5. measuring tank according to claim 1, is characterized in that, the side that described measurement plate has measuring flume is fixed together by glue and described flat board.
6. a method for making for measuring tank, is characterized in that, comprising:
1), make measuring flume: on the measurement plate of making at transparent organic material, produce multiple measuring flumes;
2), make step: produce the step of height higher than the level of described measuring flume and the level of described measurement plate in the surrounding of each measuring flume;
3), punching: get filler port in the plane of described measuring flume;
6), make hole for injecting glue: on the flat board of making at transparent organic material, produce multiple hole for injecting glue, the hole for injecting glue of described flat board is between the step of two adjacent measuring flumes of described measurement plate;
4), laminating a: side and the flat board described measurement plate to measuring flume are fixed together, and apply uniform pressure on flat board, so that the plane of the step of described measurement plate and flat board fit tightly;
5), injecting glue: gap location and hole for injecting glue place between described flat board and measurement plate, filling glue makes described flat board and measure plate to be fixed together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110386440.1A CN102521647B (en) | 2011-11-28 | 2011-11-28 | Metering tank and manufacture method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110386440.1A CN102521647B (en) | 2011-11-28 | 2011-11-28 | Metering tank and manufacture method thereof |
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| Publication Number | Publication Date |
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| CN102521647A CN102521647A (en) | 2012-06-27 |
| CN102521647B true CN102521647B (en) | 2014-06-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110386440.1A Active CN102521647B (en) | 2011-11-28 | 2011-11-28 | Metering tank and manufacture method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111982757A (en) * | 2020-05-13 | 2020-11-24 | 北京华龛生物科技有限公司 | Method for measuring number of particles in unit mass powder |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2069263U (en) * | 1990-05-23 | 1991-01-16 | 吴小庆 | Spermatozoa measuring plate |
| CN2073604U (en) * | 1990-07-30 | 1991-03-20 | 杜群军 | Blood counting board for the use of multicell |
| CN2107679U (en) * | 1991-11-05 | 1992-06-17 | 王植柔 | Spermatozoon counter |
| CN2449225Y (en) * | 2000-11-07 | 2001-09-19 | 彭钧 | External marked computer cell counter board |
| CN2493934Y (en) * | 2001-06-29 | 2002-05-29 | 郑清平 | Computer cell quantitative tester |
-
2011
- 2011-11-28 CN CN201110386440.1A patent/CN102521647B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2069263U (en) * | 1990-05-23 | 1991-01-16 | 吴小庆 | Spermatozoa measuring plate |
| CN2073604U (en) * | 1990-07-30 | 1991-03-20 | 杜群军 | Blood counting board for the use of multicell |
| CN2107679U (en) * | 1991-11-05 | 1992-06-17 | 王植柔 | Spermatozoon counter |
| CN2449225Y (en) * | 2000-11-07 | 2001-09-19 | 彭钧 | External marked computer cell counter board |
| CN2493934Y (en) * | 2001-06-29 | 2002-05-29 | 郑清平 | Computer cell quantitative tester |
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| CN102521647A (en) | 2012-06-27 |
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