CN102519883A - 一种激光染料噁嗪1高氯酸盐量子效率的测量方法 - Google Patents

一种激光染料噁嗪1高氯酸盐量子效率的测量方法 Download PDF

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CN102519883A
CN102519883A CN2011104549813A CN201110454981A CN102519883A CN 102519883 A CN102519883 A CN 102519883A CN 2011104549813 A CN2011104549813 A CN 2011104549813A CN 201110454981 A CN201110454981 A CN 201110454981A CN 102519883 A CN102519883 A CN 102519883A
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颜彩繁
赵丽娟
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Nankai University
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Abstract

一种激光染料噁嗪1高氯酸盐量子效率的测量方法。本发明依据是:对于一般激光染料,当染料分子被光激发到单线态某一电子态高能级Sn时,其退激发过程主要包括返回基态的荧光辐射跃迁、无辐射跃迁以及产生三重态的系间交叉方式。由于噁嗪1高氯酸盐的三线态量子效率极低,因此只考虑其单线态跃迁情况,即退激发过程包括荧光辐射跃迁和无辐射跃迁(见图1)。首先用光声光谱法分别测量噁嗪1高氯酸盐溶液未加焠灭剂和加入焠灭剂的光声信号。再由荧光光谱得到其荧光的平均波长,根据公式就可以计算出染料的发光量子效率。本发明不需要对仪器、光探测器进行各种校准,也不需要任何标准样品。该方法是一种测量激光染料绝对量子效率的简单、快速的方法。

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一种激光染料噁嗪1高氯酸盐量子效率的测量方法
技术领域
本发明涉及光化学和光物理技术领域。
背景技术
激光染料中激活离子的发光量子效率的测量对标定激光材料的发光效率和了解激光染料中激活离子非辐射跃迁过程都是非常重要的。但绝对量子效率的精确测量是十分困难的。目前,已建立了许多方法来测量量子效率,如:发光积分球法、荧光寿命法、量热法等。由于这些方法都要对光探测器进行绝对校准,还需要知道发光离子的浓度,以及合适的标准样品。因此,激光染料中激活离子量子效率的测量是比较复杂的。
发明内容
本发明的目的是克服现有技术存在的上述不足,提供一种测量激光染料中激活离子量子效率的方法。
本发明提供的激光染料噁嗪1高氯酸盐量子效率的测量方法,包括以下步骤:
(1)用光声光谱法测量噁嗪1高氯酸盐溶液的光声谱的中心波长λ0,并得到在此波长时未加入猝灭剂和加入猝灭剂的光声信号,两信号之比为
Figure BDA0000127300360000011
(2)用荧光光谱仪测定噁嗪1高氯酸盐溶液的荧光的平均波长
Figure BDA0000127300360000012
(3)根据公式
Figure BDA0000127300360000013
能够计算出噁嗪1高氯酸盐的荧光量子效率值Q。
本发明的测量原理
对于一般激光染料,当染料分子被光激发到单线态的某一电子态高能级Sn时,其退激发过程包括三种:返回基态的荧光辐射跃迁、无辐射跃迁以及产生三重态的系间交叉。由于噁嗪1高氯酸盐的三线态量子效率极低,因此我们只考虑其单线态的跃迁情况,即退激发过程包括荧光辐射跃迁和无辐射跃迁。噁嗪1高氯酸盐的能级跃迁图如图1所示。
当分子被波长λ0的光从S0态激发到较高能态时,由于分子能级(包括电子能级,振动能级和转动能级)的复杂性,使得退激发过程中辐射高中波长的荧光,假设荧光平均波长除去这部分荧光辐射能量外,分子最初吸收的能量的其余部分变成了分子内部的热能,而光声信号即与这部分热量成正比,所以光声信号可表示为:
PAS = K [ N βhυ 0 - nh υ ‾ ] - - - ( 1 )
式中N是光源在某一段时间内发射的波长为λ0的光子数,n是样品在这段时间内辐射的平均波长的光子数,β是样品在波长λ0处的吸收系数,K是常数,h为普朗克常数,υ0是光源的发射频率,
Figure BDA0000127300360000022
是辐射的平均频率。根据定义,荧光量子效率为:
光声信号可写成:
Figure BDA0000127300360000024
归一化的光声信号为:
I = PAS Nhυ 0 = Kβ ( 1 - Q υ ‾ υ 0 ) = Kβ ( 1 - Q λ 0 λ ) - - - ( 4 )
在染料样品中加入焠灭剂后,染料不再发光,染料分子所吸收的能量全部变成热量,光声信号为
PAS=KNβhυ                   (5)
其中β是加入猝灭剂后样品的吸收系数。归一化的光声信号为:
Figure BDA0000127300360000026
未加入猝灭剂和加入猝灭剂的光声信号之比为:
Figure BDA0000127300360000027
一般情况下,β≈β,所以光声信号之比近似为:
Figure BDA0000127300360000028
本发明的优点和积极效果:
激光染料的荧光量子效率是标志染料分子发光能力的一个极其重要的参量,其测量对于激光染料的研制和应用具有一定的指导意义。激光染料噁嗪1高氯酸盐量子效率的测量方法主要是通过对染料光声信号的测量间接地达到测量染料荧光量子效率的目的。本发明不需要对仪器、光探测器进行各种校准,也不需要任何标准样品。与目前测量荧光量子效率的发光积分球法、荧光寿命法、量热法相比,该方法是一种简单、快速测量激光染料绝对量子效率的方法。
附图说明
图1是噁嗪1高氯酸盐分子的能级跃迁图。
图2是噁嗪1高氯酸盐溶液在10-3M浓度下的光声光谱图。
图3是噁嗪1高氯酸盐在溶液10-4M浓度下的光声光谱图。
图4是噁嗪1高氯酸盐在溶液10-5M浓度下的光声光谱图。
最佳实施方式
实施例1
第一、用光声光谱法可以测得噁嗪1高氯酸盐溶液(浓度为10-3M)光声谱的中心波长λ0约为650nm(见图2)。同时,从图2可测出未加入猝灭剂和加入猝灭剂的光声信号的相对幅值,两信号的比值为0.799。
第二、用荧光光谱仪测定噁嗪1高氯酸盐溶液的荧光的平均波长
Figure BDA0000127300360000031
约为685nm。
第三、根据公式(8)可计算出噁嗪1高氯酸盐染料的荧光量子效率Q约为21.2%。
实施例2
第一、用光声光谱法可以测得噁嗪1高氯酸盐溶液(浓度为10-4M)光声谱的中心波长λ0约为650nm(见图3)。同时,由图3可测出未加入猝灭剂和加入猝灭剂的光声信号的相对幅值,两信号的比值为0.756。
第二、用荧光光谱仪测定噁嗪1高氯酸盐溶液的荧光的平均波长
Figure BDA0000127300360000032
约为685nm。
第三、根据公式(8)可计算出噁嗪1高氯酸盐染料的荧光量子效率Q约为25.7%。
实施例3
第一、用光声光谱法可以测得噁嗪1高氯酸盐溶液(浓度为10-5M)光声谱的中心波长λ0约为650nm(见图4)。同时,根据图4可测出未加入猝灭剂和加入猝灭剂的光声信号的相对幅值,两信号的比值为0.763。
第二、用荧光光谱仪测定噁嗪1高氯酸盐溶液的荧光的平均波长
Figure BDA0000127300360000033
约为685nm。
第三、根据公式(8)可计算出噁嗪1高氯酸盐染料的荧光量子效率Q约为25.0%。

Claims (1)

1.一种激光染料噁嗪1高氯酸盐量子效率的测量方法,其特征在于该方法包括以下步骤:
(1)用光声光谱法测量噁嗪1高氯酸盐溶液的光声谱的中心波长λ0,并得到在此波长下未加入猝灭剂与加入猝灭剂的光声信号,两信号之比为
Figure FDA0000127300350000011
(2)用荧光光谱仪测定噁嗪1高氯酸盐溶液的荧光的平均波长
Figure FDA0000127300350000012
(3)根据公式
Figure FDA0000127300350000013
能够计算出噁嗪1高氯酸盐的荧光量子效率值Q。
CN2011104549813A 2011-12-30 2011-12-30 一种激光染料噁嗪1高氯酸盐量子效率的测量方法 Pending CN102519883A (zh)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4006360A (en) * 1974-08-21 1977-02-01 Block Engineering, Inc. Method of discriminating between dyed particles and background fluorescence of the dye
CN1793923A (zh) * 2005-12-22 2006-06-28 复旦大学 一种水溶性CdTe/CdS核/壳型量子点的制备方法
US20090127450A1 (en) * 2004-10-26 2009-05-21 Commissariat A L'energie Atomique Method for the relative measurement of the fluorescence quantum efficiency of dyes in solution

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4006360A (en) * 1974-08-21 1977-02-01 Block Engineering, Inc. Method of discriminating between dyed particles and background fluorescence of the dye
US20090127450A1 (en) * 2004-10-26 2009-05-21 Commissariat A L'energie Atomique Method for the relative measurement of the fluorescence quantum efficiency of dyes in solution
CN1793923A (zh) * 2005-12-22 2006-06-28 复旦大学 一种水溶性CdTe/CdS核/壳型量子点的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李增发: "用光声光谱法测量噁嗪1高氯酸盐的绝对荧光量子产额", 《中国激光》, vol. 13, no. 12, 30 December 1986 (1986-12-30), pages 743 - 746 *

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