CN102517147A - Separating method of trans-fatty acid in tea seed oil - Google Patents
Separating method of trans-fatty acid in tea seed oil Download PDFInfo
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- CN102517147A CN102517147A CN2011103944393A CN201110394439A CN102517147A CN 102517147 A CN102517147 A CN 102517147A CN 2011103944393 A CN2011103944393 A CN 2011103944393A CN 201110394439 A CN201110394439 A CN 201110394439A CN 102517147 A CN102517147 A CN 102517147A
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- fatty acid
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- tea seed
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Abstract
The invention discloses a separating method of trans-fatty acid in tea seed oil. The separating method of the trans-fatty acid in the tea seed oil is characterized by comprising refrigerating tea oil refined at a high temperature at 4 DEG C for 48H, performing low-temperature low-speed centrifuging freezing to fat, collecting the liquid fat while most of solid fat is trans-fatty acid and stearic acid; then mixing silver nitrate and activated silica gel (1:5, m/m) to fill two-thirds of the volume of a chromatographic column, adding after-separation liquid fat of a column volume, performing chromatography, collecting the fat first flowing out which is mainly trans-fatty acid and stearic acid, and enabling the receiving amount to be 10% of fed fat; and then collecting other fat so that full separation of the trans-fatty acid in the fat is achieved and the tea seed oil free of trans-fatty acid is obtained. After 5 column volumes of chromatography is performed to a fat column, the column is washed with edible alcohol of 50 DEG C, eluate is recycled, the alcohol is reused, and the tea seed oil free of the trans-fatty acid can be obtained.
Description
Technical field
The present invention relates to trans fatty acid separation method in the tea seed oil.
Background technology
Tea seed oil contains 78-80% oleic acid, and the 8-12% linolic acid is prone to absorbed by human consumption, and long-term eating can
Softening cardiovascular and cerebrovascular, reducing cholesterol suppresses and cardiovascular and cerebrovascular diseases such as prevention coronary heart disease, hypertension,
It is a kind of fully natural green healthy oils.
The crude oil acid value of tea-seed is high, color is dark and contain peculiar smell, the standard that need come unstuck to crude oil, refinery practices such as depickling, decolouring, deodorization, dehydration just can reach food oils.But in oil refining process, need add acid, alkali, active white scholar; And will be in long time treatment more than 250 ℃; Could remove smell substance and moisture content such as the interior aldehyde ketone of degrease, because high temperature is to the tea seed oil effect in refining process, tea seed oil is prone to generate trans fatty acid.And trans fatty acid has serious harm to HUMAN HEALTH, takes in trans fatty acid for a long time, makes people's reduction memory, gets fat easily, is prone to cause coronary heart disease, forms thrombus easily, and influence is grown, and influences male fecundity, makes health care oil become harmful oil.The generation of control trans fatty acid simultaneously, adopts new technology to remove trans fatty acid wherein in the tea seed oil refining process, guarantees the quality and safety of tea seed oil.
Summary of the invention
The objective of the invention is to reduce the trans fatty acid in the tea oil, improve the trans fatty acid separating effect, a kind of tea seed oil that does not have trans fatty acid is provided to people.
The technical scheme that realizes above-mentioned purpose is: high temperature purified tea oil leaf oil refrigerated at 4 ℃, and 48H, the freezing grease of low temperature low-speed centrifugal, 4 ℃ of centrifuging temperatures, 1000 rev/mins of speed are collected liquid fat, and mostly solid grease is trans fatty acid and Triple Pressed Stearic Acid; Then mixed nitrate silver and activated silica gel (1: 5, m/m) fill chromatography column volume 2/3rds, add liquid fat after the separation of a column volume again; Carry out chromatography; Collect and flow out grease earlier, quantities received is 10% of an applied sample amount, is mainly trans fatty acid; And then collect all the other greases, and realize whole separation to trans fatty acid in the grease.The grease column chromatography more than 5 times the time, is washed post with 50 ℃ of edible ethanols,, and can not contained the tea seed oil of trans fatty acid eluate recovered alcohol usefulness again.
Embodiment
Below in conjunction with embodiment the present invention is done further detailed explanation.
Embodiment:
1. refrigerate tea seed oil: high temperature refining tea seed oil is refrigerated at 4 ℃ 48H.
2. low temperature low-speed centrifugal tea seed oil: 4 ℃ of centrifuging temperatures, 1000 rev/mins of speed are collected liquid fat and solid grease respectively, and solid grease is mainly trans fatty acid and Triple Pressed Stearic Acid.
3. column chromatography: Silver Nitrate and 200 order silica gel mix by quality at 1: 5, and the post of the lucifuge of packing into adds 1 column volume of liquid fat of collecting; Through column chromatography; Collect effusive grease earlier, collected volume is 10% of a last oil mass, is mainly trans fatty acid and Triple Pressed Stearic Acid; Regather the effusive grease in back, for not containing the tea seed oil of trans fatty acid.
4. wash chromatography column: behind 5 column volumes of grease column chromatography, wash post,, and can not contained the tea seed oil of trans fatty acid to eluate recovered alcohol usefulness again with 50 ℃ of edible ethanols.
Claims (4)
1. the separation method of trans fatty acid in the tea seed oil comprises high temperature refining tea seed oil carried out frozen centrifugation and to separate trans fatty acid through Silver Nitrate silica gel column chromatography grease.It is characterized in that: high temperature purified tea oil leaf oil is refrigerated at 4 ℃, 48H, the freezing grease of low temperature low-speed centrifugal, 4 ℃ of centrifuging temperatures, 1000 rev/mins of speed are collected liquid fat, and mostly solid grease is trans fatty acid and Triple Pressed Stearic Acid; Then mixed nitrate silver and activation 200 order silica gel (1: 5, m/m) fill chromatography column volume 2/3rds, add liquid fat after the separation of a column volume again; Carry out chromatography; Collect and flow out grease earlier, quantities received is 10% of an applied sample amount, is mainly trans fatty acid; And then collect all the other greases, and realize whole separation to trans fatty acid in the grease.Wash post with 50 ℃ of edible ethanols after using 5 times,, and can not contained the tea seed oil of trans fatty acid eluate recovered alcohol usefulness again.
2. according to the separation method of trans fatty acid in the described tea oil of claim 1; It is characterized in that: the refining tea seed oil is refrigerated at 4 ℃ 48H, the freezing grease of low temperature low-speed centrifugal; 4 ℃ of centrifuging temperatures; 1000 rev/mins of speed are collected liquid fat, realize trans fatty acid initial gross separation in the tea seed oil.
3. according to the separation method of trans fatty acid in the described tea oil of claim 1; It is characterized in that: through the tea seed oil of cold storage spinning; Pass through the Silver Nitrate silica gel column chromatography again, wash out earlier and be trans fatty acid, just flow out the tea seed oil that does not contain trans fatty acid then; Realization provides the tea seed oil of no trans fatty acid to whole separation of fat formula lipid acid in the grease.
4. according to the separation method of trans fatty acid in the described tea oil of claim 1; It is characterized in that: behind 5 column volumes of grease column chromatography; Wash post with 50 ℃ of edible ethanols,, and can not contained the tea seed oil of trans fatty acid eluate recovered alcohol usefulness again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103944393A CN102517147A (en) | 2011-11-25 | 2011-11-25 | Separating method of trans-fatty acid in tea seed oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103944393A CN102517147A (en) | 2011-11-25 | 2011-11-25 | Separating method of trans-fatty acid in tea seed oil |
Publications (1)
| Publication Number | Publication Date |
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| CN102517147A true CN102517147A (en) | 2012-06-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2011103944393A Pending CN102517147A (en) | 2011-11-25 | 2011-11-25 | Separating method of trans-fatty acid in tea seed oil |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262076A (en) * | 2014-09-04 | 2015-01-07 | 宁波大红鹰生物工程股份有限公司 | Method of purifying plant-derived squalene by utilizing silver nitrate silica gel chromatography |
| CN105419949A (en) * | 2015-11-04 | 2016-03-23 | 江南大学 | Method for separating and enriching trace branched-chain fatty acid |
| CN106290665A (en) * | 2016-11-02 | 2017-01-04 | 百奥森（江苏）食品安全科技有限公司 | A kind of detection method of Trans-fatty Acids in Foods |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101252844A (en) * | 2005-07-01 | 2008-08-27 | 马泰克生物科学公司 | Polyunsaturated fatty acid-containing oil product and uses and production thereof |
| CN101351534A (en) * | 2005-12-28 | 2009-01-21 | 株式会社For-C | Process for purification of plant oil without producing any trans-type fatty acid as by-product and apparatus for the process |
| CN101878820A (en) * | 2009-05-08 | 2010-11-10 | 常山富而康山茶油有限公司 | Method for refining camellia oil with high nutrient content |
-
2011
- 2011-11-25 CN CN2011103944393A patent/CN102517147A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101252844A (en) * | 2005-07-01 | 2008-08-27 | 马泰克生物科学公司 | Polyunsaturated fatty acid-containing oil product and uses and production thereof |
| CN101351534A (en) * | 2005-12-28 | 2009-01-21 | 株式会社For-C | Process for purification of plant oil without producing any trans-type fatty acid as by-product and apparatus for the process |
| CN101878820A (en) * | 2009-05-08 | 2010-11-10 | 常山富而康山茶油有限公司 | Method for refining camellia oil with high nutrient content |
Non-Patent Citations (1)
| Title |
|---|
| 倪培德: "《油脂加工技术》", 30 June 2007, 北京：化学工业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262076A (en) * | 2014-09-04 | 2015-01-07 | 宁波大红鹰生物工程股份有限公司 | Method of purifying plant-derived squalene by utilizing silver nitrate silica gel chromatography |
| CN105419949A (en) * | 2015-11-04 | 2016-03-23 | 江南大学 | Method for separating and enriching trace branched-chain fatty acid |
| CN105419949B (en) * | 2015-11-04 | 2020-01-07 | 江南大学 | Method for micro-separation and enrichment of branched chain fatty acid |
| CN106290665A (en) * | 2016-11-02 | 2017-01-04 | 百奥森（江苏）食品安全科技有限公司 | A kind of detection method of Trans-fatty Acids in Foods |
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Application publication date: 20120627 |