CN102511449A - Breeding method of amblyseius barkeri with pesticide resistance - Google Patents
Breeding method of amblyseius barkeri with pesticide resistance Download PDFInfo
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- CN102511449A CN102511449A CN2011103630610A CN201110363061A CN102511449A CN 102511449 A CN102511449 A CN 102511449A CN 2011103630610 A CN2011103630610 A CN 2011103630610A CN 201110363061 A CN201110363061 A CN 201110363061A CN 102511449 A CN102511449 A CN 102511449A
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- pasteur
- cypermethrin
- genus amblyseius
- amblyseius berlese
- chlopyrifos
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Abstract
The invention discloses a breeding method of amblyseius barkeri with pesticide resistance. The breeding method comprises the following steps: (1) simultaneously treating the amblyseius barkeri which is sensitive to pesticides with the common production pesticides, namely cypermethrin and chlorpyrifos, wherein the using amount of the cypermethrin and the chlorpyrifos during the treating is respectively sufficient to treat imagoes according to the dosage of being capable of killing 60%-90% of individuals of an amblyseius barkeri population; and (2) continuing rearing the survival individuals, and treating later generations of the survival individuals with the cypermethrin and the chlorpyrifos generation by generation till the resistance fold of the amblyseius barkeri to the two pesticides achieves 500 times of that of the first treating in the step (1) so as to get the amblyseius barkeri with the pesticide resistance. According to the breeding method disclosed by the invention, the pesticide resistance of the amblyseius barkeri to the common pesticides can be greatly improved, large-quantity death of the amblyseius barkeri caused by applying the pesticides in a field can be avoided, and the application effect of the amblyseius barkeri in the field can be further significantly improved.
Description
Technical field
The present invention relates to the control of agricultural pest field, relate in particular to the method that a kind of seed selection obtains pesticide resistance Pasteur genus amblyseius berlese.
Background technology
Pasteur's genus amblyseius berlese is the important natural enemy of pests such as multiple harmful mite, thrips.Pasteur's genus amblyseius berlese of indoor feeding is not owing to there being contacted insecticide, and is therefore extremely responsive to agricultural chemicals.The pest species that the field crops are taken place is more; Other insect of control unavoidably will be used a large amount of insecticides in the production; The result can cause discharging the mortality of Pasteur's genus amblyseius berlese; Thereby have a strong impact on the control efficiency of Pasteur's genus amblyseius berlese, and then influence Pasteur's genus amblyseius berlese and further apply insect (mite).
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of selecting not by what the agricultural chemicals typical concentrations was killed to have a pesticide resistance Pasteur genus amblyseius berlese selection.
Technical scheme of the present invention is following: a kind of pesticide resistance Pasteur's genus amblyseius berlese selection, and its key is to carry out as follows:
(1) utilize to produce go up the common insecticide cypermethrin and handle the Pasteur genus amblyseius berlese responsive to insecticide simultaneously with chlopyrifos, cypermethrin and chlopyrifos consumption are processed into mite with the dosage that kills Pasteur's genus amblyseius berlese population 60 % that die～90 % individuality respectively during processing;
(2) the individual continuation of the survival after step (1) processing raised; Utilize the offspring of cypermethrin and chlopyrifos processing survival individuality by generation; In each generation, is when handling; Cypermethrin and chlopyrifos concentration increase gradually, are standard to kill Pasteur's genus amblyseius berlese population 60 %～90 % individualities, and (cypermethrin and chlopyrifos are to Pasteur's genus amblyseius berlese lethal dose of 50 LC to 500 times of reaching all that step (1) handles for the first time of the resistant multiple of two kinds of medicaments up to Pasteur's genus amblyseius berlese
50Be respectively 1547.63 μ g/mL and 16065.57 μ g/mL), obtain having drug-fast Pasteur's genus amblyseius berlese.
Above-mentioned steps (2) gained has pesticide resistance Pasteur genus amblyseius berlese and whenever handled 1 time to keep their resistance at a distance from 30 days with mass concentration 300 times of liquid of cypermethrin dilution and 200 times of liquid of mass concentration 48% chlopyrifos dilution more than 20%.
Above-mentioned steps (1) is handled the cypermethrin and the chlopyrifos lethal dose of 50 LC of Pasteur's genus amblyseius berlese
50Be respectively 2.34 μ g/mL and 31.25 μ g/mL.
Above-mentioned when handling seed selection Pasteur genus amblyseius berlese with cypermethrin and chlopyrifos, also flour mite is carried out same treatment with cypermethrin and chlopyrifos.
Beneficial effect: through the seed selection of this method, can significantly improve the pesticide resistance of Pasteur's genus amblyseius berlese, avoid the field applying pesticide to cause the mortality of Pasteur's genus amblyseius berlese, thereby significantly improve Pasteur's genus amblyseius berlese field effect to common insecticide.In addition, this method also can be used for the resistance seed selection of other predation mite such as very peaceful mite of Nissl and cucumber genus amblyseius berlese.Therefore, the present technique application prospect is extensive.
Embodiment
[0009] (1) utilize to produce is gone up the common insecticide cypermethrin and is handled the Pasteur genus amblyseius berlese responsive to insecticide simultaneously with chlopyrifos, and cypermethrin and chlopyrifos consumption are processed into mite (cypermethrin and chlopyrifos are respectively 2.34 μ g/mL and 31.25 μ g/mL to Pasteur's genus amblyseius berlese lethal dose of 50 LC50) with the dosage of Pasteur's genus amblyseius berlese population 60%～90% individuality respectively during processing;
(2) the individual continuation of the survival after step (1) processing raised; Utilize the offspring of cypermethrin and chlopyrifos processing survival individuality by generation; In each generation, is when handling; Cypermethrin and chlopyrifos concentration increase gradually, are standard to kill Pasteur's genus amblyseius berlese population 60%～90% individuality, and (cypermethrin and chlopyrifos are to Pasteur's genus amblyseius berlese lethal dose of 50 LC to 500 times of reaching all that step (1) handles for the first time of the resistant multiple of two kinds of medicaments up to Pasteur's genus amblyseius berlese
50Be respectively 1547.63 μ g/mL and 16065.57 μ g/mL), obtain having drug-fast Pasteur's genus amblyseius berlese.Simultaneously to middle host---flour mite is also handled with cypermethrin and chlopyrifos.Gained has pesticide resistance Pasteur genus amblyseius berlese and flour mite was whenever handled 1 time to keep their resistance at a distance from 30 days with mass concentration 300 times of liquid of cypermethrin dilution and 200 times of liquid of mass concentration 48% chlopyrifos dilution more than 20%.
Claims (4)
1. pesticide resistance Pasteur genus amblyseius berlese selection is characterized in that carrying out as follows:
(1) utilize to produce go up the common insecticide cypermethrin and handle the Pasteur genus amblyseius berlese responsive to insecticide simultaneously with chlopyrifos, cypermethrin and chlopyrifos consumption are processed into mite with the dosage that kills Pasteur's genus amblyseius berlese population 60 %～90 % individuality respectively during processing;
(2) the individual continuation of the survival after step (1) processing raised; Utilize the offspring of cypermethrin and chlopyrifos processing survival individuality by generation; In each generation,, cypermethrin and chlopyrifos concentration increased gradually, are standard to kill Pasteur's genus amblyseius berlese population 60 %～90% individuality when handling; Up to Pasteur's genus amblyseius berlese the resistant multiple of two kinds of medicaments is reached 500 times that step (1) is handled for the first time, obtain having drug-fast Pasteur's genus amblyseius berlese.
2. according to the said a kind of pesticide resistance Pasteur's genus amblyseius berlese selection of claim 1, it is characterized in that: step (2) gained has pesticide resistance Pasteur genus amblyseius berlese and whenever handled 1 time to keep their resistance at a distance from 30 days with mass concentration 300 times of liquid of cypermethrin dilution and 200 times of liquid of mass concentration 48% chlopyrifos dilution more than 20%.
3. according to claim 1 or 2 said a kind of pesticide resistance Pasteur's genus amblyseius berlese selections, it is characterized in that: said step (1) is handled the cypermethrin and the chlopyrifos lethal dose of 50 LC of Pasteur's genus amblyseius berlese
50Be respectively 2.34 μ g/mL and 31.25 μ g/mL.
4. according to the said a kind of pesticide resistance Pasteur's genus amblyseius berlese selection of claim 3, it is characterized in that: said when handling seed selection Pasteur genus amblyseius berlese with cypermethrin and chlopyrifos, also flour mite is carried out same treatment with cypermethrin and chlopyrifos.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011103630610A CN102511449A (en) | 2011-11-16 | 2011-11-16 | Breeding method of amblyseius barkeri with pesticide resistance |
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| CN2011103630610A CN102511449A (en) | 2011-11-16 | 2011-11-16 | Breeding method of amblyseius barkeri with pesticide resistance |
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| CN102511449A true CN102511449A (en) | 2012-06-27 |
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| CN2011103630610A Pending CN102511449A (en) | 2011-11-16 | 2011-11-16 | Breeding method of amblyseius barkeri with pesticide resistance |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823547A (en) * | 2012-08-22 | 2012-12-19 | 福建省农业科学院植物保护研究所 | Drug-resistant neoseiulus amblyseius cucumeris strain |
| CN107711722A (en) * | 2017-11-02 | 2018-02-23 | 中国热带农业科学院环境与植物保护研究所 | It is a kind of to cultivate the method with drug-fast Tetrastichus brontispae population |
| CN113575235A (en) * | 2021-08-10 | 2021-11-02 | 内蒙古农业大学 | Method for synergistically preventing and treating Tetranychus urticae by using neoseiulus barkeri and pyrethroid medicaments |
Citations (2)
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| CN101011068A (en) * | 2007-01-24 | 2007-08-08 | 李爱华 | Use of Amblyseius barkeri for preventing and controlling harmful mites |
| CN101011046A (en) * | 2007-01-24 | 2007-08-08 | 李爱华 | Artificial breeding method of Amblyseius barkeri |
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2011
- 2011-11-16 CN CN2011103630610A patent/CN102511449A/en active Pending
Patent Citations (2)
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|---|---|---|---|---|
| CN101011068A (en) * | 2007-01-24 | 2007-08-08 | 李爱华 | Use of Amblyseius barkeri for preventing and controlling harmful mites |
| CN101011046A (en) * | 2007-01-24 | 2007-08-08 | 李爱华 | Artificial breeding method of Amblyseius barkeri |
Non-Patent Citations (6)
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| NOMIKOU M ET AL: "Phytoseiid predators as potential biological control agents for Bemisia tabaci", 《EXPERIMENTAL ＆ APPLIED ACAROLOGY》, vol. 25, no. 4, 30 April 2001 (2001-04-30), pages 271 - 279 * |
| PETRUSHOV A.Z.: "Pyrethroid resistance in the predacious miteAmblyseius barkeri", 《EPPO BULLETIN 》, vol. 22, no. 3, 31 March 1992 (1992-03-31), pages 471 - 473 * |
| 何林等: "朱砂叶螨的抗药性选育及其解毒酶活性研究", 《农药学学报》, vol. 5, no. 4, 31 December 2003 (2003-12-31), pages 23 - 29 * |
| 张倩倩等: "猎物对巴氏钝绥螨生长发育和繁殖的影响", 《华东昆虫学报》, vol. 14, no. 2, 28 February 2005 (2005-02-28), pages 165 - 168 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823547A (en) * | 2012-08-22 | 2012-12-19 | 福建省农业科学院植物保护研究所 | Drug-resistant neoseiulus amblyseius cucumeris strain |
| CN107711722A (en) * | 2017-11-02 | 2018-02-23 | 中国热带农业科学院环境与植物保护研究所 | It is a kind of to cultivate the method with drug-fast Tetrastichus brontispae population |
| CN107711722B (en) * | 2017-11-02 | 2018-11-06 | 中国热带农业科学院环境与植物保护研究所 | A method of cultivating the Tetrastichus brontispae population with drug resistance |
| CN113575235A (en) * | 2021-08-10 | 2021-11-02 | 内蒙古农业大学 | Method for synergistically preventing and treating Tetranychus urticae by using neoseiulus barkeri and pyrethroid medicaments |
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Application publication date: 20120627 |