CN102495220B - Stable reagent kit of ethanol determination liquid - Google Patents
Stable reagent kit of ethanol determination liquid Download PDFInfo
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- CN102495220B CN102495220B CN 201110347261 CN201110347261A CN102495220B CN 102495220 B CN102495220 B CN 102495220B CN 201110347261 CN201110347261 CN 201110347261 CN 201110347261 A CN201110347261 A CN 201110347261A CN 102495220 B CN102495220 B CN 102495220B
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Abstract
The invention discloses a stable reagent kit of ethanol determination liquid, which is a liquid double reagent formed by reagent I and reagent II, wherein the reagent I includes buffer solution (pH8.5-9.5)0.05-0.5M, reaction promoter 1-200g/L, stabilizer 0.1-10g/L, and preservative 0.1-10g/L; and reagent II includes buffer solution (pH6.0-7.0)0.05-0.5M, beta-coenzymeI 0.1-10g/L, alcohol dehydrogenase 0.2-200KU/L, compound stabilizer 0.1-10g/L, activator 0.1g-100g/L, and preservative 0.1-10g/L. The invention has the advantages that the reagent kit is highly accurate, precise and stable, can be stably stored at least for 12 months away from the light, and can fully satisfy the requirements of clinic detection.
Description
Technical field
The invention belongs to medical test determination techniques field, be specifically related to a kind of stable reagent kit of ethanol determination liquid.
Background technology
Ethanol (alcohol) is present in most of alcoholic drink, and the speed of its metabolism depends primarily on the content of body endoenzyme, has larger individual difference.As everyone knows, low alcohol consumption can stimulate circulation, but a large amount of Ethanol intake can cause alcohol dependence, can cause spirit and Analysis of multi-organic functional damages, and is wherein common with the harm to liver function and moral function, also serious.The habituation of ethanol, abuse and dependence are one of social economy the most serious in world today's scope and public health problem, and in western countries, the alcohol related liver diseases has become dead major reason.Therefore the blood alcohol determining plays a part very important in acute alcoholism diagnosis and Forensic Identification.
In biological sample, the method for quantitatively determining of ethanol mainly contains chemical colorimetry, enzyme terminal colorimetric analysis, homogeneous EIA method, vapor-phase chromatography and expired air ethanol analysis method etc.Vapor-phase chromatography is considered to the reference method of alcohol determining.Vapor-phase chromatography at first with the NaCl saturated solution in the container of a sealing with serum or diluting blood sample, be the gas of volatilization on the top of closed container.Sampling makes sample cleaning like this, and is very little or there is no a matrix impact.Can complete the quantitative of peak value by typical curve.Shortcoming is that instrument is expensive, and the complicated inconvenience of formality is promoted.The enzyme process autoanalyzer method divides Alcoholic oxidase and alcohol dehydrogenase enzyme process, and it is simple that oxidation enzyme process reagent set becomes, and sample does not need pre-treatment, but poor specificity, methyl alcohol disturbs greatly measuring, and false positive can occur.Ethanol dehydrogenase is relatively special for ethanol, can not play catalyzed reaction with methyl alcohol, acetone or Virahol etc., therefore accuracy and precision are higher, and it can be measured on ultraviolet/visible light analyser or half, automatic clinical chemistry analyzer, speed is fast, good with the vapor-phase chromatography dependency, its principle is as follows:
Ethanol+nadide
+ 
Acetaldehyde+DPNH+H
+
Generate DPNH with nadide when being generated acetaldehyde according to ethanol by the alcohol dehydrogenase oxydasis, the DPNH that produces can be directly in 340 nm place colorimetric detection, utilize the enzyme process of DPNH similar with many other enzyme process just in use, can complete on the existing instrument in laboratory, this is medically having suitable sensitivity.
It is high that enzyme assay has specificity, but advantage simple and quick, accurate safe automated analysis, but because ethanol dehydrogenase is extremely unstable, but high density stable existence several days only 5 ℃ time the in nearly neutral pure water, unstable in acid (pH is lower than 6.0) and alkalescence (pH is greater than 8.5) solution, and heavy metal and at present generally the reaction promotor example hydrochloric acid Urea,amino-of use, glycine etc. has obvious restraining effect to it; In addition, the nadide that uses in reagent is unstable under this reaction conditions; Therefore must to make powdered reagent could prolonged preservation stable for this reagent, also must use as early as possible after redissolution, otherwise comparatively fast can make a big impact to the reagent performance due to ethanol dehydrogenase and nadide degradation speed in the preservation process, can't satisfy the clinical detection needs.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of accuracy, precision all better, good stability, 2～8 ℃ keep in Dark Place and can stablize 12 months at least, can satisfy the stable reagent kit of ethanol determination liquid of Clinical Laboratory requirement fully.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: stable reagent kit of ethanol determination liquid, this test kit are the liquid-type double reagent that is comprised of reagent 1 and reagent 2, wherein
Reagent 1 each component and concentration are:
Damping fluid (pH 8.5-9.5) 0.05 ~ 0.5 M (mol/L)
Reaction promotor 1 ~ 200 g/L
Stablizer 0.1 ~ 10 g/L
Sanitas 0.1 ~ 10 g/L;
Reagent 2 each components and concentration are:
Damping fluid (pH 6.0-7.0) 0. 05 ~ 0.5 M
β-nadide (claiming again nadide or Reduced nicotinamide-adenine dinucleotide) 0.1 ~ 10 g/L
Ethanol dehydrogenase 0.2 ~ 200 KU/L
One package stabilizer 0.1 ~ 10 g/L
Activator 0.1 ~ 100 g/L
Sanitas 0.1 ~ 10 g/L.
Damping fluid in mentioned reagent 1 of the present invention is Tri(Hydroxymethyl) Amino Methane Hydrochloride damping fluid, 3-Pehanorm-2-hydroxy-propanesulfonic acid damping fluid, 4-hydroxyethyl piperazine propanesulfonic acid damping fluid, a kind of in sodium phosphate buffer etc., preferred 4-hydroxyethyl piperazine propanesulfonic acid damping fluid.
Reaction promotor in mentioned reagent 1 of the present invention is one or more (i.e. two kinds or the two or more mixing, the proportion relation between several components of mixing does not require) in succinic acid, semicarbazide hydrochloride, glycine, two glycosides peptide, acetaldehyde dehydrogenase etc.
Stablizer in mentioned reagent 1 of the present invention be polysorbas20, tween 80, Brij-35 (
Brij-35), one or more in the nonionogenic tenside such as Triton X-100 (triton x-100).
Damping fluid in mentioned reagent 2 of the present invention is a kind of in hydroxyethyl piperazine second thiosulfonic acid damping fluid, 4-hydroxyethyl piperazine propanesulfonic acid damping fluid, Tri(Hydroxymethyl) Amino Methane Hydrochloride damping fluid, 3-Pehanorm-2-hydroxy-propanesulfonic acid damping fluid etc., preferred hydroxyethyl piperazine second thiosulfonic acid damping fluid, pH 6.0-7.0.
Above-mentioned one package stabilizer is one or more and the enzyme combining stabilizer M03(ES-W03 in gsh, halfcystine, sucrose, trehalose or serum albumin), enzyme combining stabilizer M07 (ES-W07), enzyme combining stabilizer M08(ES-W08) a kind of composition in (be one package stabilizer be from gsh, halfcystine, sucrose, trehalose or serum albumin one or more again with enzyme combining stabilizer M03, enzyme combining stabilizer M07, a kind of the mixing together in enzyme combining stabilizer M08).
Above-mentioned activator is one or more (i.e. two kinds or the two or more mixing, the proportion relation between several components of mixing does not require) in mercaptoethanol, DTT, halfcystine and oxalic acid tetraacethyl disodium.
Sanitas of the present invention is sodium azide or proclin series sanitas (as Proclin300) etc.
The compound method of reagent 1 of the present invention and reagent 2 is ordinary method, namely according to above-mentioned formula rate, reagent 1 and described each component of reagent 2 is added respectively to mix to stir evenly separately after distilled water respectively to get final product.
The test condition of the ethanol in kit measurement sample of the present invention is as follows: temperature: 30-37 ℃; The cuvette optical path is 1.0 cm.Detect predominant wavelength 340 nm.
The method of using ethanol in alcohol determining kit measurement sample of the present invention is as follows: sample (calibration tube is made sample with calibration object) reagent adding 1 mixing, 30-37 ℃ is read absorbance A 0 after hatching 3-5 min, add immediately reagent 2 mixings, after 30-37 ℃ of reaction 5 min, read absorbance A 1, Δ A=A1-A0.Sample consumption 2 μ l wherein, reagent 1 consumption 200 μ l, reagent 2 consumption 50 μ l.
In kit measurement sample of the present invention, ethanol content calculates as follows:
Outstanding substantive distinguishing features of the present invention and marked improvement are mainly manifested in:
(1) the present invention by add one package stabilizer in reagent 2, has protected ethanol dehydrogenase and the nadide stability in pH 6.0-7.0 damping fluid, and reagent 2-8 ℃ preservation of the present invention can be stablized 12 months at least, can satisfy the Clinical Laboratory requirement fully.
(2) the present invention by add activator in reagent 2, has evaded the restraining effect of reaction promotor to ethanol dehydrogenase to a certain extent.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is further described, but the present invention is not limited to these embodiment.
Detection principle of the present invention is as follows: generate DPNH with nadide when being generated acetaldehyde according to ethanol by the alcohol dehydrogenase oxydasis, the DPNH that produces can be directly in 340 nm place colorimetric detection, the absorbancy velocity of variation is directly proportional to alcohol concn in sample.
Embodiment 1
Reagent 1 each component and concentration are:
Sodium phosphate buffer (pH 8.5) 0.1 M
Semicarbazide hydrochloride 2 g/L
Polysorbas20 1 g/L
Proclin300 0.1 g/L；
Reagent 2 each components and concentration are:
Tri(Hydroxymethyl) Amino Methane Hydrochloride damping fluid (pH 6.0) 0. 05 M
Cozymase 0.5 g/L
Alcohol dehydrogenase 20 KU/L
Trehalose (one package stabilizer) 10 g/L
ES-W03 (one package stabilizer) 10 g/L
Mercaptoethanol (activator) 2 g/L
Proclin300 0.2 g/L。
The compound method of reagent 1 and reagent 2 is ordinary method, and namely reagent 1 and the described component of reagent 2 add respectively to mix to stir evenly separately after distilled water and get final product.
Embodiment 2
Reagent 1 each component and concentration are:
Tri(Hydroxymethyl) Amino Methane Hydrochloride damping fluid (pH 9.5) 0.05 M
Two glycosides peptide 5 g/L
Tween 80 10 g/L
Proclin300 0.2 g/L；
Reagent 2 each components and concentration are:
3-Pehanorm-2-hydroxy-propanesulfonic acid damping fluid (pH 6.5) 0.1 M
Cozymase 5 g/L
Ethanol dehydrogenase 50 KU/L
Halfcystine (one package stabilizer) 5 g/L
ES-W07 (one package stabilizer) 1 g/L
DTT (activator) 10 g/L
Sodium azide 0.1 g/L.
The preparation method of embodiment 2 test kits is with embodiment 1.
Embodiment 3
Reagent 1 each component and concentration are:
4-hydroxyethyl piperazine propanesulfonic acid damping fluid (pH 9.5) 0.1 M
Glycine 10 g/L
Brij-35 5 g/L
Sodium azide 0.1 g/L
Reagent 2 each components and concentration are:
Hydroxyethyl piperazine second thiosulfonic acid damping fluid (pH 7.0) 0.1 M
Cozymase 5 g/L
Ethanol dehydrogenase 50 KU/L
Gsh (one package stabilizer) 2 g/L
ES-W08 (one package stabilizer) 2 g/L
Halfcystine (activator) 10 g/L
Oxalic acid tetraacethyl disodium (activator) 1 g/L
Proclin300 0.1 g/L。
The preparation method of embodiment 3 test kits is with embodiment 1.
Reference examples 1
Prepare by embodiment 3, but do not add one package stabilizer gsh and ES-W08.
Reference examples 2
Prepare by embodiment 3, but do not add activator oxalic acid tetraacethyl disodium.
The raw material that embodiment is used unless otherwise indicated, is commercially available industrial goods.
The test condition of the ethanol in kit measurement sample of the present invention is as follows: temperature: 30-37 ℃; The cuvette optical path is 1.0 cm.Detect predominant wavelength 340 nm.
The method of using ethanol in alcohol determining kit measurement sample of the present invention is as follows: sample (calibration tube is made sample with calibration object) adds the R1 mixing, 30-37 ℃ is read absorbance A 0 after hatching 3-5 min, adds immediately the R2 mixing, after 30-37 ℃ of reaction 5 min, read absorbance A 1, Δ A=A1-A0.Sample consumption 2 μ l wherein, reagent 1 consumption 200 μ l, reagent 2 consumption 50 μ l.
In kit measurement sample of the present invention, ethanol content calculates as follows:
Describe below in conjunction with the performance of form to embodiment of the present invention gained test kit.
1, speed of reaction
With embodiment 3, the reagent of reference examples 1 and reference examples 2 preparations detects sample, and it is as shown in table 1 that the sample absorbancy changes (Δ A mensuration-Δ A is blank) detected result:
Table 1
| Group | Reference examples 1 | Reference examples 2 | Embodiment 3 |
| Δ A mensuration-Δ A is blank | 1.6123 | 1.0368 | 1.6261 |
Above data obviously show, the reagent react speed of reference examples 1 of the present invention and embodiment 3 preparations is very fast, adds activator and has evaded to a certain extent the restraining effect of reaction promotor to ethanol dehydrogenase.
2, test kit stability experiment
(1) 37 ℃ of accelerated stability experiment
The reagent of reference examples 1-2 and embodiment 3 preparations is carried out 37 ℃ of accelerated stabilities experiments (but 37 ℃ of accelerated stability tests 7 days simulating reality 4-8 ℃ preserved 1 year), and it is as shown in table 2 that the sample absorbancy changes (Δ A mensurations-Δ A blank) detected result:
Table 2
| Time | Reference examples 1 | Reference examples 2 | Embodiment 3 |
| The 1st day | 1.6431 | 1.1322 | 1.6481 |
| 37 ℃ 3 days | 1.0214 | 1.1164 | 1.5946 |
| 37 ℃ 5 days | 0.6532 | 0.9845 | 1.4987 |
| 37 ℃ 7 days | 0.2847 | 0.9063 | 1.4652 |
| The 7th day decline % | 82.7％ | 20.0% | 11.1% |
(2) 4-8 ℃ of stability experiment
The reagent of reference examples 1-2 and embodiment 3 preparations was preserved 1 year at 4-8 ℃, and it is as shown in table 3 that the sample absorbancy changes (Δ A mensuration-Δ A is blank) result:
Table 3
| Month | Reference examples 1 | Reference examples 2 | Embodiment 3 |
| 1 | 1.6324 | 1.2104 | 1.6358 |
| 3 | 1.2546 | 1.1865 | 1.6124 |
| 5 | 0.9894 | 1.1654 | 1.5864 |
| 7 | 0.7605 | 1.1320 | 1.5623 |
| 9 | 0.5231 | 1.1006 | 1.5417 |
| 12 | 0.3746 | 1.0836 | 1.5103 |
Above data declaration, the reagent stability of reference examples 2 of the present invention and embodiment 3 preparations is better, one package stabilizer add the stability of having protected ethanol dehydrogenase and nadide.
Claims (3)
1. stable reagent kit of ethanol determination liquid is characterized in that: the liquid-type double reagent of this test kit for being formed by reagent 1 and reagent 2, wherein
Reagent 1 each component and concentration are:
Reagent 2 each components and concentration are:
Damping fluid in reagent 1 is a kind of in Tri(Hydroxymethyl) Amino Methane Hydrochloride damping fluid, 3-Pehanorm-2-hydroxy-propanesulfonic acid damping fluid, 4-hydroxyethyl piperazine propanesulfonic acid damping fluid, sodium phosphate buffer;
Reaction promotor in reagent 1 is one or more in succinic acid, semicarbazide hydrochloride, glycine, two glycosides peptide, acetaldehyde dehydrogenase;
Stablizer in reagent 1 is one or more in polysorbas20, tween 80, Brij-35, Triton X-100;
Damping fluid in reagent 2 is a kind of in hydroxyethyl piperazine second thiosulfonic acid damping fluid, 4-hydroxyethyl piperazine propanesulfonic acid damping fluid, Tri(Hydroxymethyl) Amino Methane Hydrochloride damping fluid, 3-Pehanorm-2-hydroxy-propanesulfonic acid damping fluid;
One package stabilizer described in reagent 2 is one or more and the enzyme combining stabilizer M03 in gsh, halfcystine, sucrose, trehalose or serum albumin, enzyme combining stabilizer M07, and a kind of mixing in enzyme combining stabilizer M08 is used;
Activator described in reagent 2 is one or more in mercaptoethanol, DTT, halfcystine, oxalic acid tetraacethyl disodium;
Described sanitas is sodium azide or proclin series sanitas.
2. stable reagent kit of ethanol determination liquid according to claim 1, it is characterized in that: the damping fluid in reagent 1 is 4-hydroxyethyl piperazine propanesulfonic acid damping fluid.
3. stable reagent kit of ethanol determination liquid according to claim 1, it is characterized in that: the damping fluid in reagent 2 is hydroxyethyl piperazine second thiosulfonic acid damping fluid.
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| CN 201110347261 CN102495220B (en) | 2011-11-07 | 2011-11-07 | Stable reagent kit of ethanol determination liquid |
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| CN 201110347261 CN102495220B (en) | 2011-11-07 | 2011-11-07 | Stable reagent kit of ethanol determination liquid |
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| CN102495220B true CN102495220B (en) | 2013-11-06 |
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| CN106885803B (en) * | 2015-12-16 | 2019-06-28 | 山东博科生物产业有限公司 | A kind of strong antijamming capability and the high enzyme process ethyl alcohol detection reagent of accuracy |
| CN110187096B (en) * | 2019-05-23 | 2020-12-15 | 苏州喜全软件科技有限公司 | Sucrose-containing biological product stabilizer for ELISA kit and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5759539A (en) * | 1995-06-06 | 1998-06-02 | Georgia Research Foundation, Inc. | Method for rapid enzymatic alcohol removal |
| CN1553185A (en) * | 2003-05-28 | 2004-12-08 | 上海保康生物高科技有限公司 | Double enzyme reagent strip for quantitatively determining saliva alcohol sensor by glassifying process |
| CN101324628A (en) * | 2007-06-13 | 2008-12-17 | 苏州艾杰生物科技有限公司 | Method for determining ethanol concentration and ethanol diagnosis/determination reagent kit |
| JP4252198B2 (en) * | 2000-07-31 | 2009-04-08 | 西川ゴム工業株式会社 | Hard weather car roof weather strip |
| CN101419172A (en) * | 2008-09-25 | 2009-04-29 | 上海锦隆生物科技有限公司 | A kind of ethanol content detecting reagent in saliva |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5461984A (en) * | 1977-10-27 | 1979-05-18 | Agency Of Ind Science & Technol | Electrochemical measuring method of alcohol concentrations and electrode used for the same |
| JPH04252198A (en) * | 1991-01-25 | 1992-09-08 | Japan Tobacco Inc | Determination of alcohol |
| DE10313971A1 (en) * | 2003-03-27 | 2004-10-21 | Degussa Ag | Coupled cofactor-dependent enzymatic reaction system |
-
2011
- 2011-11-07 CN CN 201110347261 patent/CN102495220B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5759539A (en) * | 1995-06-06 | 1998-06-02 | Georgia Research Foundation, Inc. | Method for rapid enzymatic alcohol removal |
| JP4252198B2 (en) * | 2000-07-31 | 2009-04-08 | 西川ゴム工業株式会社 | Hard weather car roof weather strip |
| CN1553185A (en) * | 2003-05-28 | 2004-12-08 | 上海保康生物高科技有限公司 | Double enzyme reagent strip for quantitatively determining saliva alcohol sensor by glassifying process |
| CN101324628A (en) * | 2007-06-13 | 2008-12-17 | 苏州艾杰生物科技有限公司 | Method for determining ethanol concentration and ethanol diagnosis/determination reagent kit |
| CN101419172A (en) * | 2008-09-25 | 2009-04-29 | 上海锦隆生物科技有限公司 | A kind of ethanol content detecting reagent in saliva |
Non-Patent Citations (4)
| Title |
|---|
| 乙醇含量的酶法测定;柳畅先等;《分析测试学报》;20030731;第22卷(第4期);第96-97页 * |
| 改良乙醇脱氢酶法测定血清中微量乙醇;胡云良等;《中国卫生检验杂志》;20080131;第18卷(第1期);第47-48页 * |
| 柳畅先等.乙醇含量的酶法测定.《分析测试学报》.2003,第22卷(第4期), |
| 胡云良等.改良乙醇脱氢酶法测定血清中微量乙醇.《中国卫生检验杂志》.2008,第18卷(第1期), |
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Address after: 315104 Zhejiang city of Ningbo province Yinzhou District Qiming Road No. 299 Patentee after: MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Address before: 315104 Ningbo, Yinzhou, Central District, No. Qiming South Road, No. 299 Patentee before: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. |
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Application publication date: 20120613 Assignee: Ningbo Shengyuan Biotechnology Co., Ltd. Assignor: MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Contract record no.: X2022330000361 Denomination of invention: Stable ethanol assay liquid kit Granted publication date: 20131106 License type: Common License Record date: 20220809 |
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