CN102492661A - Preparation method and application of immunization preparation for controlling novel duck reovirus - Google Patents
Preparation method and application of immunization preparation for controlling novel duck reovirus Download PDFInfo
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- CN102492661A CN102492661A CN2011104610648A CN201110461064A CN102492661A CN 102492661 A CN102492661 A CN 102492661A CN 2011104610648 A CN2011104610648 A CN 2011104610648A CN 201110461064 A CN201110461064 A CN 201110461064A CN 102492661 A CN102492661 A CN 102492661A
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Abstract
The invention discloses a novel duck reovirus strain NDRV-JM85 with a preservation number of CCTCC NO: V201127. The novel duck reovirus strain NDRV-JM85 is used as a virus seed to inoculate an allantoic cavity of a muscovy duck embryo and obtain a virus-containing allantoic fluid, or inoculate an MDEF cell to obtain cytotoxin when a CPE reaches 75%. The virus liquid is inactivated by formaldehyde and added with a white oil adjuvant, and the mixture is mixed thoroughly to from a safe and effective oil emulsion inactivated vaccine for novel duck reovirus. The vaccine is used to immunize a laying hen three times, with an immunization interval of 21 days and 1.0 ml for each hen at each time. Eggs laid 21-63 days after the three immunizations are gained, and extracted yolk and a proper amount of disinfected normal saline is prepared into a hyperimmune antibody preparation for the novel duck reovirus. A duckling without an NDRV maternal antibody is injected with the antibody preparation at 1-2 days old for 0.5ml / duckling, or a duckling with a maternal antibody is injected with the antibody preparation at 10 days old for 1.0 ml / duckling, so as to prevent and control the novel duck reovirus. The invention applies to large-scale production under a GMP condition.
Description
Technical field
The present invention relates to a kind of preparation method and application that prevents and treats the sick immunological reagent of novel duck reovirus.
Background technology
Novel duck reovirus sick (being commonly called as duck hemorrhagic necrosis hepatitis) has been since 2005; Provinces such as Fujian China, Guangdong and Zhejiang kind duck, half kind of duck and sheldrake mass-sending give birth to a kind of with the irregular necrosis of liver and hemorrhage mix, cardiac muscle is hemorrhage, spleen enlargement patch shape downright bad, kidney and the fabricius bursa are hemorrhage etc. is the eqpidemic disease of principal character; This disease does not have obvious seasonality; Various kind ducks (like a kind duck, half kind of duck, sheldrake, Beijing duck etc.) all can be taken place, and the trend that increases is year by year arranged; The morbidity age in days is about 3～25 ages in days, and is wherein in the majority with 5～10 ages in days, the course of disease 5～7d, sickness rate 5%～20%, mortality ratio 2%～15%, age in days heal little or during accompanying infection its sickness rate mortality ratio higher, the provisions duck is already caused the certain economic loss.The Fujian Province Academy Of Agricultural Sciences Animal Husbandry And Veterinary Medicine Institute from viral physics and chemistry biology, animal return the cause of disease that confirms duck hemorrhagic necrosis hepatitis on test, genomic characterization and the molecular level first be a kind of novel duck reovirus that is different from a kind duck reovirus (MDRV), Avianreovirus (ARV) (Novel Duck Reovirus, NDRV).Up to now, this disease does not still have the effectively preventing method.
Summary of the invention
One of the object of the invention provides duck reovirus NDRV-JM85 strain:
Duck reovirus NDRV-JM85 strain; To be novel duck reovirus through biotechnology go down to posterity causes weak seed selection and forms; Be deposited in Chinese typical culture collection center (China Center for Type Culture Collection; Be called for short CCTCC), July 29 2011 preservation day, deposit number: CCTCC NO:V201127.Depositary institution CCTCC address is in Chinese Wuhan City Wuhan University.
Two of the object of the invention provides the method for preparing the sick immunological reagent of the novel duck reovirus of control with duck reovirus NDRV-JM85 strain.Described preparation method is following:
Duck reovirus NDRV-JM85 strain is cultivated in a kind DEF (MDEF) or 11 ages in days kind duck embryo, prepares novel duck reovirus inactivated vaccine with oily adjuvant after gathering in the crops viral liquid deactivation.Use 3 immune laying hens of this vaccine, results 3 are exempted from back 21 days to 63 days egg, extract yolk and are prepared into the sick immunological reagent of novel duck reovirus with an amount of sterile saline.
Three of the object of the invention provides the application with the sick immunological reagent of the novel duck reovirus of control of duck reovirus NDRV-JM85 strain preparation:
The duckling of no NDRV maternal antibody is at the 1-2 age in days, and every bipennate muscle is annotated the immunological reagent of 05ml; The duckling that contains the NDRV maternal antibody is at 10 age in days left and right sides injecting immune preparations, 1.0ml/ plumage; Duckling can be resisted the infection of NDRV behind the injecting immune preparation.
Duckling inoculates an amount of immunological reagent of the present invention, can prevent duck hemorrhagic necrosis hepatitis (novel duck reovirus is sick)
Advantage of the present invention: the present invention processes novel duck reovirus first and prepares immunological reagent, and said preparation prepares easy, and cost is low, and security is good, for the novel duck reovirus disease of anti-system provides a kind of safe and effective procedure.
Embodiment
Below in conjunction with specific embodiment the present invention is further specified.(but not being limitation of the present invention).
Duck reovirus NDRV-JM85 strain is novel duck reovirus through biotechnology goes down to posterity and causes weak seed selection and form.Be deposited in CCTCC, July 29 2011 preservation day, deposit number: CCTCC NO:V201127.
Above-mentioned duck reovirus NDRV-JM85 strain is kind of a poison, inoculation kind duck embryo allantoic cavity, allantoic cavity is inoculated 11 ages in days kind duck embryo virus of proliferation, gathers in the crops toxic allantoic fluid; Or inoculation kind DEF individual layer, to reach 75% harvested cell when above malicious when cytopathy; With above-mentioned virus with formalin-inactivated after, in suitable proportion (like 1: 1 ratio) and oily adjuvant mixing, be developed into inactivated vaccine.Laying hen is opened in the inactivated vaccine immunity, the 1.0ml/ plumage, immunity is 3 times altogether, each 21 days at interval.Results 3 are exempted from back 21 days to 63 days egg, and aseptic extraction yolk is with an amount of sterile saline mixing and filter packing, is the sick immunological reagent of novel duck reovirus.
Concrete grammar is following:
(1) antigen prepd: with kind duck embryo of JM85 virus strain inoculation kind DEF (MDEF) or 11 ages in days, the harvested cell pathology reaches the allantoic fluid poison of dead kind duck embryo behind 75% cell toxicant or the 24h, and-20 ℃ frozen.As the TCID of virus to a kind DEF (MDEF)
50>=10
-60During/0.1ml, can be used for the deactivation of virus.With the allantoic fluid or the cell toxicant of results, press 0.06% of TV and add pure formaldehyde, 37 ℃ of deactivation 24h.The viral liquid of getting after the deactivation is inoculated 9 age in days SPF chicken embryos or 11 ages in days kind duck embryo, and a chicken embryo and a kind duck embryo should all be survived.
(2) vaccine production: inactivation of viruses liquid is mixed by 1: 1 with oily adjuvant, stir and process the water-in-oil emulsion vaccine.This vaccine steriling test is answered asepsis growth.The healthy susceptible kind duck of 7 ages in days, behind the vaccine of every plumage neck subcutaneous injection 1.0ml, the inoculation duck should all survive, and it is all normal to search for food, drink water, grow etc.; Cut open duck extremely at random, internal organ such as the heart, liver, spleen are answered no abnormality seen.
(3) preparation of immunological reagent: use the above vaccine immunity laying hen for preparing 3 times, each 21 days at interval, every plumage per injection 1.0ml.Collection 3 is exempted from the back height that contained novel duck reovirus antibody in 21 days to 63 days and is exempted from egg, after the eggshell washing and sterilizing, and aseptic technique; Separate yolk, add a certain proportion of sterile saline (antibody titer according in the yolk is decided) and carry out homogenate, and every milliliter adds penicillium mould, each 1000 unit of Streptomycin sulphate; Thiomersalate 1.0/ ten thousand filters, in the packing sterilizing bottle;-20 ℃ of refrigerations are subsequent use, are prepared into the yolk antibody immunological reagent.
(4) mensuration of immunological reagent index of correlation
4.1 steriling test: undertaken by the existing the People's Republic of China " veterinary drug allusion quotation ", all do not have growths such as bacterium, mould, mycoplasma.
4.2 safety examination: with young kind duck 10 plumages of the healthy susceptible of 1 age in days, 1.0 milliliters of every plumage leg muscle injections were observed 20 days, all strong living.
4.3 attack poison protection check: a 1-2 age in days kind duck is divided into 2 groups, every group 10 plumage, and wherein preparation 0.5ml/ plumage is penetrated in the intramuscular injection of immunological reagent group leg, and sterilization hanks liquid 0.5ml/ plumage is penetrated in the intramuscular injection of control group leg; In injection the last the 3rd day, inoculate NDRV poison by force respectively for two groups, the 0.5ml/ plumage is observed the searching for food of each group test kind duck, is drunk water, situation such as morbidity, death, and cuts open 3 plumages extremely at random for every group in attacking poison back the 7th day, observes pathologies such as the heart, liver, spleen.The result: the strong poison of immunological reagent group artificial challenge back is in the whole observation period, searches for food, drinking-water etc. is all normal, and cuts open 3 plumages extremely at random, the equal no abnormality seen of each organ.Behind the strong poison of control group artificial challenge, a kind duck shows as material feeding, drinking-water reduces or death, and whole viewing duration body weight is significantly less than the preparation group; Pathologies such as cuing open and examine visible liver, spleen is significantly hemorrhage and downright bad, and heart is hemorrhage.
(5) application of immunological reagent:
The duckling of no NDRV maternal antibody is at the 1-2 age in days, and every bipennate muscle is annotated the preparation of 0.5ml; There is the duckling of NDRV maternal antibody can be at 10 age in days left and right sides injecting immune preparations, 1.0ml/ plumage.
(6) clinical application effect test-results:
Select the control test that 3 meat duck plants carry out immunological reagent in the popular epidemic-stricken area of NDRV.Duckling is at the 1-2 age in days, and every plumage is inoculated NDRV preparation 0.5ml, tracing observation 45 days, and untoward reaction does not appear in the test duckling, and duckling does not all have NDRV and infects in whole feeding period.And periphery does not take the plant of above measure to have NDRV morbidity and dead always.Further proof said preparation safety, effective can be used for preventing clinically duck hemorrhagic necrosis hepatitis (novel duck reovirus is sick).
Claims (3)
1. duck reovirus NDRV-JM85 strain is deposited in CCTCC, preservation date on July 29th, 2011, deposit number: CCTCC NO:V201127.
2. prepare the method for the sick immunological reagent of the novel duck reovirus of control with the described duck reovirus of claim 1 NDRV-JM85 strain, it is characterized in that described preparation method is following:
Inoculate 11 ages in days kind duck embryo virus of proliferation with duck reovirus NDRV-JM85 strain allantoic cavity, gather in the crops toxic allantoic fluid; Or inoculation kind DEF individual layer, to reach 75% harvested cell when above malicious when cytopathy; With above-mentioned virus with formalin-inactivated after, by suitable proportion and oily adjuvant mixing, be developed into inactivated vaccine; Laying hen is opened in the inactivated vaccine immunity, the 1.0ml/ plumage, immunity is 3 times altogether, each 21 days at interval.Results 3 are exempted from back 21 days to 63 days egg, and aseptic extraction yolk is with an amount of sterile saline mixing and filter packing, is the sick immunological reagent of novel duck reovirus.
3. with the application of the sick immunological reagent of the novel duck reovirus of the control of the described duck reovirus of claim 1 NDRV-JM85 strain preparation.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102631674A (en) * | 2012-03-02 | 2012-08-15 | 福建省农业科学院畜牧兽医研究所 | Novel muscovy duck reovirus disease vaccine and application thereof |
| CN108498794A (en) * | 2018-05-22 | 2018-09-07 | 山东农业大学 | A kind of inactivated vaccine and preparation method thereof of prevention novel duck reovirus |
| CN108611441A (en) * | 2018-05-22 | 2018-10-02 | 山东农业大学 | It is a kind of detection novel duck reovirus PCR kit for fluorescence quantitative and application |
| CN108743932A (en) * | 2018-07-03 | 2018-11-06 | 重庆永健生物技术有限责任公司 | A kind of inactivated vaccine preparation method for preventing duck reovirus |
| CN108794627A (en) * | 2018-07-03 | 2018-11-13 | 重庆永健生物技术有限责任公司 | A kind of preparation method of duck reovirus refined vitelline antibody |
| CN110663647A (en) * | 2019-10-25 | 2020-01-10 | 福建农林大学 | Muscovy duck reovirus synbiotic infection morbidity model and construction method thereof |
| CN111000992A (en) * | 2019-12-19 | 2020-04-14 | 广州渔跃生物技术有限公司 | Triple inactivated vaccine for duck reovirus, duck tembusu virus and duck adenovirus and preparation method thereof |
| CN112080477A (en) * | 2020-05-09 | 2020-12-15 | 重庆三杰众鑫生物工程有限公司 | Preparation method and application of duck reovirus and antigen-antibody complex thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110590946B (en) * | 2019-08-29 | 2021-05-18 | 广东渔跃生物技术有限公司 | Preparation method of duck reovirus egg yolk antibody |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1426814A (en) * | 2001-12-19 | 2003-07-02 | 福建省农业科学院畜牧兽医研究所 | Foreign duck liver ichthyophthirius active vaccine and its preparation method |
| CN1528454A (en) * | 2001-12-19 | 2004-09-15 | 福建省农业科学院畜牧兽医研究所 | Foreign duck liver ichthyophthirius active vaccine and preparation method thereof |
-
2011
- 2011-12-29 CN CN201110461064.8A patent/CN102492661B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1426814A (en) * | 2001-12-19 | 2003-07-02 | 福建省农业科学院畜牧兽医研究所 | Foreign duck liver ichthyophthirius active vaccine and its preparation method |
| CN1528454A (en) * | 2001-12-19 | 2004-09-15 | 福建省农业科学院畜牧兽医研究所 | Foreign duck liver ichthyophthirius active vaccine and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 陈仕龙 等: "新型鸭呼肠孤病毒分离株的致病性研究", 《西北农林科技大学学报（自然科学版）》, vol. 38, no. 4, 30 April 2010 (2010-04-30) * |
| 陈少莺 等: "新型鸭呼肠孤病毒研究进展", 《中国畜牧兽医学会禽病学分会第十五次学术研讨会论文集》, 1 October 2010 (2010-10-01) * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102631674A (en) * | 2012-03-02 | 2012-08-15 | 福建省农业科学院畜牧兽医研究所 | Novel muscovy duck reovirus disease vaccine and application thereof |
| CN108498794A (en) * | 2018-05-22 | 2018-09-07 | 山东农业大学 | A kind of inactivated vaccine and preparation method thereof of prevention novel duck reovirus |
| CN108611441A (en) * | 2018-05-22 | 2018-10-02 | 山东农业大学 | It is a kind of detection novel duck reovirus PCR kit for fluorescence quantitative and application |
| CN108498794B (en) * | 2018-05-22 | 2020-07-07 | 山东农业大学 | Inactivated vaccine for preventing and treating novel duck reovirus and preparation method thereof |
| CN108743932A (en) * | 2018-07-03 | 2018-11-06 | 重庆永健生物技术有限责任公司 | A kind of inactivated vaccine preparation method for preventing duck reovirus |
| CN108794627A (en) * | 2018-07-03 | 2018-11-13 | 重庆永健生物技术有限责任公司 | A kind of preparation method of duck reovirus refined vitelline antibody |
| CN110663647A (en) * | 2019-10-25 | 2020-01-10 | 福建农林大学 | Muscovy duck reovirus synbiotic infection morbidity model and construction method thereof |
| CN111000992A (en) * | 2019-12-19 | 2020-04-14 | 广州渔跃生物技术有限公司 | Triple inactivated vaccine for duck reovirus, duck tembusu virus and duck adenovirus and preparation method thereof |
| CN112080477A (en) * | 2020-05-09 | 2020-12-15 | 重庆三杰众鑫生物工程有限公司 | Preparation method and application of duck reovirus and antigen-antibody complex thereof |
| CN112080477B (en) * | 2020-05-09 | 2023-05-16 | 重庆三杰众鑫生物工程有限公司 | Duck reovirus and preparation method and application of antigen-antibody complex thereof |
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| CN102492661B (en) | 2014-01-08 |
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