CN102488100A - Compound-probiotics-containing feed additive and preparation method thereof - Google Patents
Compound-probiotics-containing feed additive and preparation method thereof Download PDFInfo
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- CN102488100A CN102488100A CN2011104354552A CN201110435455A CN102488100A CN 102488100 A CN102488100 A CN 102488100A CN 2011104354552 A CN2011104354552 A CN 2011104354552A CN 201110435455 A CN201110435455 A CN 201110435455A CN 102488100 A CN102488100 A CN 102488100A
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Abstract
The invention relates to a compound-probiotics-containing feed additive and a preparation method thereof. The compound-probiotics-containing feed additive comprises the following raw material components: cured corn husk, corn steep liquor, oligo-isomaltose, mannan-oligosaccharide and galactooligosacchride as well as Saccharomyces boulardii bacterial liquid, Bacillus Licheniformis bacterial liquid and lactobacillus bacterial liquid. The feed additive provided by the invention overcomes the defect that raw material of the traditional feed additive is single, quantity of survival probiotics in the product is high, and the feed additive provided by the invention has the characteristics of safety, environmental degradation and low cost.
Description
Technical field
The present invention relates to a kind of feed addictive that contains composite prebiotics and preparation method thereof; Particularly a kind of utilize that corn processing byproduct and multiple prebiotics make contain feed addictive of composite prebiotics and preparation method thereof, belong to field of feed additive technology.
Background information
China is animal husbandry big country, the quality of life that the sound development of animal husbandry affects the livelihood of every family.At present, along with improving constantly of food security consciousness, people have proposed increasingly high requirement to the security of livestock and poultry product; And the antibiotic that large-scale aquaculture exists excessively uses; Caused everybody concern, national correlation department is also recognized the seriousness of these potential problems, just actively puts into effect corresponding measure; Reduce the addition of antibiotic in feed, encourage the research and the application of substitute technology.
Probiotic is a kind of functional viable bacteria feed additive; It is through the beneficial bacteria of intestinal tract external a large amount of cultivation animals; After being eaten by animal; Play and replenish animal, improve the state of animal metabolism dysfunction, resistance against diseases decline owing to taking the effect that antibiotic or disease reason cause beneficial bacteria of intestinal tract quantity to reduce.The characteristics that it has nontoxic, the no resistance to the action of a drug, has no side effect, be a kind of safe, efficient, can partly substitute antibiotic product.Probiotic has become a developing direction of following feed addictive.
Prebiotics is one type and is proved to be the growth that can stimulate intestinal beneficial bacterium, suppresses the functional oligosaccharide of harmful bacteria growing, and it can play indirect facilitation to intestinal beneficial bacterium crowd's formation and structure, is used with probiotic, and effect is very obvious.
Corn itself is important feed-use grain; And corn producing industry also has sizable scale in China, and corn is in being processed into the process of starch, except that producing the major product cornstarch; Also produce a large amount of accessory substances, comprise maize peel, corn protein powder, corn steep liquor and maize germ wet goods.At present these accessory substances are directly sold as feed or as the raw material of industrial microorganism fermentation, and therefore, can there be the problem of security in their application in feed addictive.
Summary of the invention
The present invention is directed to the deficiency of prior art, a kind of feed addictive that contains composite prebiotics and preparation method thereof is provided.
Technical scheme of the present invention is following:
A kind of feed addictive that contains composite prebiotics, raw material components is following, is weight portion:
80～100 parts of slaking maize peels, 10～20 parts of corn steep liquors, 0.5～5 part of oligoisomaltose, 0.5～4 part of Oligomeric manna sugar, 2～4 parts of galactooligosaccharides; And,
Cell concentration is 1.5 * 10
9Cfu/ml～2.5 * 10
95～15 parts of the Bradley yeast of cfu/ml (Saccharomyces boulardii) bacterium liquid, cell concentration is 5.0 * 10
8Cfu/ml～8.5 * 10
83～10 parts of the bacillus licheniformis of cfu/ml (Bacillus Licheniformis), cell concentration is 1.5 * 10
9Cfu/ml～2.5 * 10
95～15 parts of the Bacillus acidi lacticis of cfu/ml (lactobacillus);
Said oligoisomaltose, Oligomeric manna sugar, galactooligosaccharide are all in pure article.
Preferably, raw material components is following, is weight portion:
85～100 parts of slaking maize peels, 10～12 parts of corn steep liquors, 3～5 parts of oligoisomaltoses, 0.8～1 part of Oligomeric manna sugar, 2.5～3 parts of galactooligosaccharides; And,
Cell concentration is 1.5 * 10
9Cfu/ml～2.5 * 10
98～10 parts of the saccharomyces boulardii liquid of cfu/ml, cell concentration are 5.0 * 10
8Cfu/ml～8.5 * 10
84～6 parts of the bacillus licheniformis of cfu/ml, cell concentration are 1.5 * 10
9Cfu/ml～2.5 * 10
910～12 parts of the Bacillus acidi lacticis of cfu/ml.
Above-mentioned slaking maize peel be with maize peel under 0.08MPa～0.12MPa pressure, 115 ℃～121 ℃, carry out steam heated 30～50min under the condition of pH4.0～5.8.The cured matter surface of slaking maize peel is destroyed, and the slaking maize peel increases than the maize peel adsorptivity, and the macromolecular polysaccharide constituents in the maize peel more is prone to degraded, can improve the probio quantity of probiotic.
Said corn steep liquor is the corn steep liquor of the dense 15～30wt% of slurry.
Above-mentioned Bradley yeast (Saccharomyces boulardii), bacillus licheniformis (Bacillus Licheniformis), Bacillus acidi lactici (lactobacillus) are common commercially available bacterial strain.
The above-mentioned preparation method who contains the feed addictive of composite prebiotics, step is following:
(1) gets the Bradley yeast,,, obtain cell concentration 1.5 * 10 then through 28 ℃～32 ℃, 160r/min～180r/min shaking table enlarged culture 30h～36h through 28 ℃～32 ℃ activation culture 30h～38h
9Cfu/ml～2.5 * 10
9The saccharomyces boulardii liquid of cfu/ml;
(2) get bacillus licheniformis,,, obtain cell concentration 5.0 * 10 then through 34 ℃～36 ℃, 160r/min～200r/min shaking table enlarged culture 10h～15h through 35 ℃～38 ℃ activation culture 10h～16h
8Cfu/ml～8.5 * 10
8The bacillus licheniformis bacterium liquid of cfu/ml;
(3) extracting lactic acid bacillus through 32 ℃～38 ℃ activation culture 20h～30h, then through 30 ℃～36 ℃ enlarged culture 18h～24h, obtains cell concentration 1.5 * 10
9Cfu/ml～2.5 * 10
9The Bacillus acidi lactici bacterium liquid of cfu/ml;
(4) in proportion; The saccharomyces boulardii liquid that step (1) is made, the bacillus licheniformis bacterium liquid that step (2) makes, Bacillus acidi lactici bacterium liquid and slaking maize peel, corn steep liquor, oligoisomaltose, Oligomeric manna sugar, the galactooligosaccharide that step (3) makes mix, and promptly get.
Activation culture in the said step (1), nutrient media components is following, all is weight percentage:
Glucose 4%～6%, peptone 0.8%～1.2%, agar 1.5%～2%, excess water.
Activation culture in the said step (2), nutrient media components is following, all is weight percentage:
Peptone 0.8%～1.2%, NaCl 1%～1.2%, yeast extract 0.5%～0.8%, agar 1.5%～2%, excess water, pH7.0～7.5.
Activation culture in the said step (3), nutrient media components is following, all is weight percentage:
Enlarged culture in the said step (1), nutrient media components is following, all is weight percentage:
Enlarged culture in the said step (2), nutrient media components is following, all is weight percentage:
Peptone 0.8%～1.2%, NaCl0.8%～1%, corn steep liquor 8%～10%, excess water, pH 7.0～7.5.
Enlarged culture in the said step (3), nutrient media components is following, all is weight percentage:
Above-mentioned culture medium all adopts the condition of 121 ℃ of sterilization 20min to carry out sterilization treatment.
Said oligoisomaltose, Oligomeric manna sugar, galactooligosaccharide are common commercially available food additives.
Beneficial effect
Feed addictive of the present invention not only contains three kinds of important probios; Also add corn processing byproduct slaking maize peel, corn steep liquor packing material as probiotic; Added multiple functional prebiotics simultaneously; Strengthen the action effect of probiotic, not only overcome the single defective of existing feed addictive raw material, and the survival bacterium amount of probio is high in the product; This feed addictive also has the low characteristics of safety, environmentally degradable and cost in addition, can significantly improve antibiotic and excessively use the influence to aquaculture, improves the security and the practicality of animal products.
Description of drawings
Fig. 1 is bacillus licheniformis (Bacillus Licheniformis) fermented and cultured growth curve;
Fig. 2 is Bacillus acidi lactici (lactobacillus) fermented and cultured growth curve;
Fig. 3 is Bradley yeast (Saccharomyces boulardii) fermented and cultured growth curve;
The specific embodiment
Below in conjunction with embodiment the present invention is done further elaboration, but institute of the present invention protection domain is not limited thereto.
Bacillus licheniformis among the embodiment (Bacillus Licheniformis) is available from Jinan Rui Feng bioengineering Co., Ltd; Bacillus acidi lactici (lactobacillus) is available from Jinan Rui Feng bioengineering Co., Ltd; Bradley yeast (Saccharomyces boulardii) is available from Jinan Rui Feng bioengineering Co., Ltd; Maize peel is available from Dezhou Fu Yuan biological starch Co., Ltd; Corn steep liquor is available from Dezhou Fu Yuan biological starch Co., Ltd; Oligoisomaltose is available from Jinan, Shandong Zhong Run bioengineering Co., Ltd; Oligomeric manna sugar is available from Chengdu standing grain day bio tech ltd, and galactooligosaccharide is available from Jinan, Shandong Zhong Run bioengineering Co., Ltd.
Embodiment 1
A kind of feed addictive that contains composite prebiotics, raw material components is following:
Slaking maize peel 200g starches the corn steep liquor 30g of dense 20wt%, oligoisomaltose 7g, Oligomeric manna sugar 1.5g, galactooligosaccharide 2g; And, 1.75 * 10
9The saccharomyces boulardii liquid 20g of cfu/ml cell concentration, 8.5 * 10
8The bacillus licheniformis 10g of cfu/ml cell concentration, 2.5 * 10
9The Bacillus acidi lactici 20g of cfu/ml cell concentration;
Above-mentioned slaking maize peel be with maize peel under 0.1MPa pressure, 120 ℃, carry out steam heated 50min under the condition of pH4.0～5.8, make.
The above-mentioned preparation method who contains the feed addictive of composite prebiotics; Step is following: Bradley yeast (Saccharomyces boulardii) is got in (1), through 30 ℃ of activation culture 36h, till mycoderm covers whole media surface; Get 2 ring bacterial classifications then; Be seeded to activation medium,, obtain cell concentration 1.5 * 10 through 30 ℃, 180r/min shaking table enlarged culture 36h
9Cfu/ml～2.5 * 10
9The bacterium liquid of cfu/ml;
Said activation culture, nutrient media components is following, all is weight percentage:
4% glucose, 1% peptone, 2% agar, excess water.
Said enlarged culture, nutrient media components is following, all is weight percentage:
(2) get bacillus licheniformis (Bacillus Licheniformis), through 37 ℃ of activation culture 12h, till mycoderm covers whole media surface; Get 3 ring bacterial classifications then; Be seeded to activation medium,, obtain cell concentration 5.0 * 10 through 37 ℃, 180r/min shaking table enlarged culture 12h
8Cfu/ml～8.5 * 10
8The bacterium liquid of cfu/ml;
Said activation culture, nutrient media components is following, all is weight percentage:
1% peptone, 1%NaCl, 0.5% yeast extract, 2% agar, excess water, pH7.5.
Said enlarged culture, nutrient media components is following, all is weight percentage:
1% peptone, 1%NaCl, 10% corn steep liquor, excess water, pH 7.5.
(3) extracting lactic acid bacillus (lactobacillus) through 37 ℃ of activation culture 24h, is got 3 ring bacterial classifications then, is seeded to activation medium, through 37 ℃ of constant temperature enlarged culture 24h, obtains cell concentration 1.5 * 10
9Cfu/ml～2.5 * 10
9The bacterium liquid of cfu/ml;
Said activation culture, nutrient media components is following, all is weight percentage:
Said enlarged culture, nutrient media components is following, all is weight percentage:
(4) in proportion; The saccharomyces boulardii liquid that step (1) is made, the bacillus licheniformis bacterium liquid that step (2) makes, Bacillus acidi lactici bacterium liquid and slaking maize peel, corn steep liquor, oligoisomaltose, Oligomeric manna sugar, the galactooligosaccharide that step (3) makes mix; Pack in the polybag; Vacuumize, make the feed addictive that contains composite prebiotics.
Above-mentioned culture medium all adopts the condition of 121 ℃ of sterilization 20min to carry out sterilization treatment.
The detection method of viable bacteria adopts the probiotic sample of regularly going bail for and depositing, and dilution back spread plate was cultivated about 48 hours, and the counting colony counts is calculated amount of viable bacteria according to extension rate, and the testing result of the survival rate of probiotic viable bacteria is seen table 1:
Table 1
Preservation condition: vacuum packaging, preserve in 4 ℃ of refrigerators.
Embodiment 2:
A kind of feed addictive that contains composite prebiotics, raw material components is following:
Slaking maize peel 200g starches the corn steep liquor 20g of dense 20wt%, oligoisomaltose 8g, Oligomeric manna sugar 6g, galactooligosaccharide 4g; And, 1.75 * 10
9The saccharomyces boulardii liquid 20g of cfu/ml cell concentration, 8.5 * 10
8The bacillus licheniformis 10g of cfu/ml cell concentration, 2.5 * 10
9The Bacillus acidi lactici 20g of cfu/ml cell concentration;
The preparation of slaking maize peel is with embodiment 1.
The preparation method of feed addictive who contains composite prebiotics is with embodiment 1.
The detection method of viable bacteria is with instance 1, and the testing result of the survival rate of probiotic viable bacteria is seen table 2:
Table 2
Preservation condition: vacuum packaging, preserve in 4 ℃ of refrigerators.
Embodiment 3:
A kind of feed addictive that contains composite prebiotics, raw material components is following:
Slaking maize peel 200g starches the corn steep liquor 10g of dense 20wt%, oligoisomaltose 8g, Oligomeric manna sugar 6g, galactooligosaccharide 4g; And, 1.75 * 10
9The saccharomyces boulardii liquid 20g of cfu/ml cell concentration, 8.5 * 10
8The bacillus licheniformis 10g of cfu/ml cell concentration, 2.5 * 10
9The Bacillus acidi lactici 20g of cfu/ml cell concentration;
The preparation of slaking maize peel is with embodiment 1.
The preparation method of feed addictive who contains composite prebiotics is with embodiment 1.
The detection method of viable bacteria is with instance 1, and the testing result of the survival rate of probiotic viable bacteria is seen table 3:
Table 3
Preservation condition: vacuum packaging, preserve in 4 ℃ of refrigerators.
Claims (8)
1. a feed addictive that contains composite prebiotics is characterized in that raw material components is following, is weight portion:
80～100 parts of slaking maize peels, 10～20 parts of corn steep liquors, 0.5～5 part of oligoisomaltose, 0.5～4 part of Oligomeric manna sugar, 2～4 parts of galactooligosaccharides; And,
1.5 * 10
9Cfu/ml～2.5 * 10
95～15 parts of the Bradley yeast of cfu/ml cell concentration (Saccharomyces boulardii) bacterium liquid, 5.0 * 10
8Cfu/ml～8.5 * 10
83～10 parts of the bacillus licheniformis of cfu/ml cell concentration (Bacillus Licheniformis), 1.5 * 10
9Cfu/ml～2.5 * 10
95～15 parts of the Bacillus acidi lacticis of cfu/ml cell concentration (lactobacillus);
Said oligoisomaltose, Oligomeric manna sugar, galactooligosaccharide are all in pure article.
2. feed addictive as claimed in claim 1 is characterized in that raw material components is following, is weight portion:
85～100 parts of slaking maize peels, 10～12 parts of corn steep liquors, 3～5 parts of oligoisomaltoses, 0.8～1 part of Oligomeric manna sugar, 2.5～3 parts of galactooligosaccharides; And,
1.5 * 10
9Cfu/ml～2.5 * 10
98～10 parts of the saccharomyces boulardii liquid of cfu/ml cell concentration, 5.0 * 10
8Cfu/ml～8.5 * 10
84～6 parts of the bacillus licheniformis of cfu/ml cell concentration, 1.5 * 10
9Cfu/ml～2.5 * 10
910～12 parts of the Bacillus acidi lacticis of cfu/ml cell concentration.
3. according to claim 1 or claim 2 feed addictive is characterized in that, the slaking maize peel be with maize peel under 0.08MPa～0.12MPa pressure, 115 ℃～121 ℃, carry out steam heated 30～50min under the condition of pH4.0～5.8.
4. according to claim 1 or claim 2 feed addictive is characterized in that, said corn steep liquor is the corn steep liquor of the dense 15～30wt% of slurry.
5. claim 1 or the 2 described preparation methods that contain the feed addictive of composite prebiotics is characterized in that step is following:
(1) gets the Bradley yeast,,, obtain cell concentration 1.5 * 10 then through 28 ℃～32 ℃, 160r/min～180r/min shaking table enlarged culture 30h～36h through 28 ℃～32 ℃ activation culture 30h～38h
9Cfu/ml～2.5 * 10
9The saccharomyces boulardii liquid of cfu/ml;
(2) get bacillus licheniformis,,, obtain cell concentration 5.0 * 10 then through 34 ℃～36 ℃, 160r/min～200r/min shaking table enlarged culture 10h～15h through 35 ℃～38 ℃ activation culture 10h～16h
8Cfu/ml～8.5 * 10
8The bacillus licheniformis bacterium liquid of cfu/ml;
(3) extracting lactic acid bacillus through 32 ℃～38 ℃ activation culture 20h～30h, then through 30 ℃～36 ℃ enlarged culture 18h～24h, obtains cell concentration 1.5 * 10
9Cfu/ml～2.5 * 10
9The Bacillus acidi lactici bacterium liquid of cfu/ml;
(4) in proportion; The saccharomyces boulardii liquid that step (1) is made, the bacillus licheniformis bacterium liquid that step (2) makes, Bacillus acidi lactici bacterium liquid and slaking maize peel, corn steep liquor, oligoisomaltose, Oligomeric manna sugar, the galactooligosaccharide that step (3) makes mix, and promptly get.
6. preparation method as claimed in claim 5 is characterized in that, the activation culture in the said step (1), and nutrient media components is following, all is weight percentage:
Glucose 4%～6%, peptone 0.8%～1.2%, agar 1.5%～2%, excess water;
Enlarged culture in the said step (1), nutrient media components is following, all is weight percentage:
Glucose 2%～4%, corn steep liquor 8%～10%, CaCO
30.2%～0.3%, excess water, pH 4.0～5.0.
7. preparation method as claimed in claim 5 is characterized in that, the activation culture in the said step (2), and nutrient media components is following, all is weight percentage:
Peptone 0.8%～1.2%, NaCl 1%～1.2%, yeast extract 0.5%～0.8%, agar 1.5%～2%, excess water, pH7.0～7.5;
Enlarged culture in the said step (2), nutrient media components is following, all is weight percentage:
Peptone 0.8%～1.2%, NaCl0.8%～1%, corn steep liquor 8%～10%, excess water, pH 7.0～7.5.
8. preparation method as claimed in claim 5 is characterized in that, the activation culture in the said step (3), and nutrient media components is following, all is weight percentage:
Glucose 2%～4%, peptone 0.8%～1.2%, beef extract 1%～1.2%, yeast extract 0.5%～0.6%, citric acid diamines 0.1%～0.2%, dipotassium hydrogen phosphate 0.15%～0.2%, MgSO
47H
2O 0.05%～0.06%, MnSO
4H
2O0.02%～0.03%, Tween 80 0.1%～0.12%, sodium acetate 0.2%～0.5%, agar 1.5%～2%, excess water, pH6.5～7.0;
Enlarged culture in the said step (3), nutrient media components is following, all is weight percentage:
Glucose 2%～4%4%, peptone 0.8%～1.2%, beef extract 1%～1.2%, yeast extract 0.3%～0.5%, citric acid diamines 0.1%～0.2%, dipotassium hydrogen phosphate 0.1%～0.2%, MgSO
47H
2O 0.05%～0.06%, MnSO
4H
2O 0.02%～0.03%, Tween 80 0.1%～0.12%, and sodium acetate 0.2%～0.5%, agar 1.5%～2%, excess water, pH 6.8.
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| CN103141666A (en) * | 2013-03-28 | 2013-06-12 | 山东轻工业学院 | Method for producing microbe feed probiotics by using white spirit vinasse |
| MD4216C1 (en) * | 2012-08-15 | 2013-11-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Strain of Saccharomyces cerevisiae yeast - producer of mannans |
| CN104146149A (en) * | 2014-05-20 | 2014-11-19 | 东北农业大学 | Preparation method of wet corn fiber feed |
| CN104642734A (en) * | 2013-11-15 | 2015-05-27 | 中国科学院过程工程研究所 | Broiler feed additive and use thereof |
| CN105249506A (en) * | 2015-09-11 | 2016-01-20 | 安徽丰原马鞍山生物化学有限公司 | Guniting corn bran preparation method and production system |
| CN106387336A (en) * | 2016-08-31 | 2017-02-15 | 西安若翰生物饲料有限公司 | Corn slurry fermented feed and production method thereof |
| CN107072247A (en) * | 2014-07-29 | 2017-08-18 | 百通生物技术公司 | Animal feed composition and feed addictive |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD4216C1 (en) * | 2012-08-15 | 2013-11-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Strain of Saccharomyces cerevisiae yeast - producer of mannans |
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| CN104642734A (en) * | 2013-11-15 | 2015-05-27 | 中国科学院过程工程研究所 | Broiler feed additive and use thereof |
| CN104642734B (en) * | 2013-11-15 | 2018-05-22 | 中国科学院过程工程研究所 | A kind of broiler chicken feed additive and its application |
| CN104146149A (en) * | 2014-05-20 | 2014-11-19 | 东北农业大学 | Preparation method of wet corn fiber feed |
| CN107072247A (en) * | 2014-07-29 | 2017-08-18 | 百通生物技术公司 | Animal feed composition and feed addictive |
| CN105249506A (en) * | 2015-09-11 | 2016-01-20 | 安徽丰原马鞍山生物化学有限公司 | Guniting corn bran preparation method and production system |
| CN106387336A (en) * | 2016-08-31 | 2017-02-15 | 西安若翰生物饲料有限公司 | Corn slurry fermented feed and production method thereof |
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