CN102477420A - Cell control method for hydrophilic modification on low free energy surface - Google Patents
Cell control method for hydrophilic modification on low free energy surface Download PDFInfo
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- CN102477420A CN102477420A CN2010105536002A CN201010553600A CN102477420A CN 102477420 A CN102477420 A CN 102477420A CN 2010105536002 A CN2010105536002 A CN 2010105536002A CN 201010553600 A CN201010553600 A CN 201010553600A CN 102477420 A CN102477420 A CN 102477420A
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Abstract
The invention relates to a technology integrating surface chemistry, biomaterials science, a micro-production technology, plasma chemistry and the like in the field of interdiscipline subjects, in particular to a surface chemistry microcosmic mode which is used for carrying out surface chemical modification on a hydrophobic low free energy surface to form a hydrophilic surface with high free energy, so as to control space distribution, shape and size of cells on the matrix surface. The cell control method disclosed by the invention has wide application prospects in the biomedical engineering fields, such as tissue engineering, biosensors of cells, biochips and biological micro electromechanical systems (Bio MEMs).
Description
Technical field
The invention belongs to interdisciplinary fields such as surface chemistry, biomaterial, micro production technology, plasma chemistry, the concrete cell control method that relates to a kind of low free energy surface hydrophilic modification.
Background technology
Adhesion and the growth of cell on stromal surface depends on the chemical property of stromal surface strongly, comprises surface free energy and parent/hydrophobicity etc.So, modify through the medelling of stromal surface chemical property, can control the spatial distribution of cell on stromal surface.In addition, through the control of stromal surface model shape with size, so as to the shape and the size of control cell, this is a major technique characteristic of the present invention.Yet, also do not see at present the report of constructed characteristic in the document, the technology with same effect that can see only has the gold surface alkanethiol salt self-assembled monolayer of the Whitesides of department of chemistry of Harvard University professor laboratory development technological.Its principle is; Through modification, utilize the characteristic of oligomeric ethylene glycol group arrestin matter absorption, in conjunction with the micro production technology to alkanethiol salt self-assembled monolayer terminal functional groups; Make the background surface of arrestin matter absorption; And the alkanethiol salt self-assembled monolayer island that has other terminal functional groups such as methyl of protein adsorption is supported in introducing above that, thereby reaches the effect that control protein adsorption sheaf space distributes, and and then control cell spaces distribution and cell shape and big or small purpose.
Above-mentioned two kinds of technology, its ultimate principle are obviously different.Alkanethiol salt self-assembled monolayer technology is mainly utilized the characteristic of oligomeric ethylene glycol group arrestin matter absorption, and the mechanism of this characteristic is still undistinct at present, it is believed that with the High Density Packaging of surface group oligomeric ethylene glycol cause sterically hindered relevant.
Summary of the invention
The present invention is directed to the current situation of above-mentioned prior art; The present invention proposes a kind of new technical scheme; Be to utilize very low this surface chemistry of some material surface free energy; In conjunction with utilizing micro production technology and plasma surface modification technique, on the low surface free energy material surface, introduce high surface free energy zone, thereby reach control cell spatial distribution from the teeth outwards with and shape and big or small purpose.
Summary of the invention the object of the present invention is to provide a kind of through carrying out surface chemical modification in hydrophobic low free energy stromal surface; Form hydrophilic and have the surface chemistry microscopic mode of high surface free energy, with the method for spatial distribution, shape and the size of control cell on stromal surface.Another object of the present invention is above-mentioned method is used in biomedical engineering field, cell biological sensor, biochip and biological microelectromechanical-systems.
The present invention is through experiment confirm, and on the low free energy surface of strong-hydrophobicity, cell is difficult to adhere to usually.So, can select low free energy material of many strong-hydrophobicities such as tetrafluoroethylene, YSR 3286, what they were applicable to all that the present invention states carries out the material of surface chemical modification as matrix; Wherein, (polydimethylsiloxane is PDMS) as one of representative of low surface free energy material for preferred YSR 3286; Utilize the technology and the method for subjects such as surface chemistry, biomaterial, micro production technology, plasma chemistry,, be unfavorable on the surface of cell adhesion at its low free energy; Centrifugal coating photoresist material; Mask is arranged on photoresist material again, after the exposure photoresist material is removed in development, expose exposed PDMS surface.Through the graphic introducing of high surface free energy, plasma treatment is carried out finishing to exposed PDMS surface, forms hydrophilic high free energy surface.Remove not exposure photoresist material again, make the PDMS surface-rendering of masked coverage, the result has formed the purpose zone of the spatial positioning of control cell adhesion, cell shape and size on the PDMS surface.For reaching technological step that the object of the invention takes shown in Fig. 1.
Description of drawings
Introduce hydrophilic high free energy regional development and technology flow process on Fig. 1, hydrophobic low free energy YSR 3286 (PDMS) surface.
The variation of Fig. 2, YSR 3286 (PDMS) surface free energy after plasma treatment, surface free energy system calculates through the contact angle of measuring water and methylene iodide.Surface free energy is in treatment time positive correlation.
The hydrophilic variation of Fig. 3, YSR 3286 surface microscopic after oxygen plasma treatment.Volatilization formed after the demonstration system of pattern condensed with water vapour from the teeth outwards.Plasma treatment makes exposed YSR 3286 (PDMS) surface become hydrophilic.The water droplet volatilization is slower, and both unprocessed zone, background area on every side, because the hydrophobicity on surface, water droplet evaporates very soon.Circular diameter is 50 microns among the left figure, and the square length of side is 45 microns among the right figure.
Fig. 4, the sterically defined realization of cell adhesion.The spatial positioning of cell adhesion.The left side is the PDMS surface through plasma treatment; But the right side is same PDMS surface is that photoresist material is covered when making public; Behind the inoculating cell, cell is only promptly adhering to growth in the left side through the plasma treatment zone, and cell can not adhere on the PDMS surface, right side.
The control of Fig. 5, cell shape and size.Cell presents the arrangement of rule among the figure, is shown as circular array or rectangular array.Circular diameter is 50 microns, and the rectangle length of side is 3O * 70 micron.Though on a lot of circles 2 cells are arranged, can make its area only be enough to sprawl a cell through the reducing of circular diameter.
Embodiment
In order to understand the present invention better, but and unrestricted the present invention, further explain through following examples.
Embodiment:
1, the introducing of microscopic mode on the PDMS surface
The PDMS that adopts is U.S. Dow corning Company products Sylgard 184.Can be by l: 5-1: 60 mixed glue and solidifying agent be heating and curing then.The introducing of microscopic mode system reaches through traditional photoetching process on the PDMS surface.Concrete scheme does; Centrifugal coating one deck positive corrosion-resisting agent (positive photoresist) (S-9912 on the PDMS surface; Shipley company produces), 80 ℃ were toasted 25 minutes, made public with respective sources (200W high voltage mercury lamp radiation 90 seconds) through mask then; And develop with the hungry aqueous solution of 2 % tetramethyl-hydroxides and to remove exposed photoresist, the medelling that can on the PDMS surface, form unexposed photoresist encapsulates layer.After the exposed photoresist development is removed, expose exposed PDMS surface.
2, plasma surface is modified
Plasma body has very high energy and reactive behavior, can with exposed PDMS surface reaction, the result shows as hydrophilic high free energy zone.Plasma intensity and action time can be selected voluntarily, and modification result and this two parameters are related.
3, the spatial positioning of cell adhesion
The PDMS of subregion plasma treatment adopts the sterilization of 75 % or soaked in absolute ethyl alcohol, according to conventional cultivation technique inoculation ox articular chondrocytes and cultivate.
4, the control of cell shape and size
Have the mask graph similar through making with the cell yardstick, according to above-mentioned flow process, i.e. the shape of may command cell and size.
Claims (3)
1. the one kind low cell control method that the free energy surface hydrophilic is modified is characterized in that: adopt a kind of surface to have the material that hydrophobicity is hanged down free energy, at its surperficial centrifugal coating photoresist material; Coverage mask on photoresist material, exposure is developed and is removed the exposure photoresist material; Expose exposed stromal surface,, exposed surface is carried out finishing, form hydrophilic high free energy surface through the graphic introducing of high surface free energy; Remove not exposure photoresist material of masked coverage again, the surface has formed a kind of method of control cell adhesion area of space location, cell shape and size as a result.
2. the cell control method of modifying according to the described a kind of low free energy surface hydrophilic of claim 1, the material that wherein said a kind of surface has the low free energy of hydrophobicity can be tetrafluoroethylene or YSR 3286.
3. the cell control method of modifying according to the described a kind of low free energy surface hydrophilic of claim 1 is characterized in that the energy of the graphic introducing of wherein said high surface free energy comprises plasma body, ionic fluid, PRK, electron beam or X light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105536002A CN102477420A (en) | 2010-11-22 | 2010-11-22 | Cell control method for hydrophilic modification on low free energy surface |
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| CN2010105536002A CN102477420A (en) | 2010-11-22 | 2010-11-22 | Cell control method for hydrophilic modification on low free energy surface |
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| CN102477420A true CN102477420A (en) | 2012-05-30 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104338333A (en) * | 2014-05-28 | 2015-02-11 | 中国科学院力学研究所 | Space water droplet positioning substrate and preparation method thereof |
| CN110203878A (en) * | 2019-05-27 | 2019-09-06 | 东南大学 | Single layer nano particle dimer and poly preparation based on hydrophobe array |
| CN113073029A (en) * | 2021-03-17 | 2021-07-06 | 长春长光辰英生物科学仪器有限公司 | Infiltration modified cell sorting chip for laser induced transfer and sorting method |
-
2010
- 2010-11-22 CN CN2010105536002A patent/CN102477420A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104338333A (en) * | 2014-05-28 | 2015-02-11 | 中国科学院力学研究所 | Space water droplet positioning substrate and preparation method thereof |
| CN110203878A (en) * | 2019-05-27 | 2019-09-06 | 东南大学 | Single layer nano particle dimer and poly preparation based on hydrophobe array |
| CN113073029A (en) * | 2021-03-17 | 2021-07-06 | 长春长光辰英生物科学仪器有限公司 | Infiltration modified cell sorting chip for laser induced transfer and sorting method |
| CN113073029B (en) * | 2021-03-17 | 2023-03-21 | 长春长光辰英生物科学仪器有限公司 | Infiltration modified cell sorting chip for laser induced transfer and sorting method |
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Application publication date: 20120530 |