CN102477102A - Method for applying bletilla striata polysaccharide to hemostatic material - Google Patents
Method for applying bletilla striata polysaccharide to hemostatic material Download PDFInfo
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- CN102477102A CN102477102A CN2010105534558A CN201010553455A CN102477102A CN 102477102 A CN102477102 A CN 102477102A CN 2010105534558 A CN2010105534558 A CN 2010105534558A CN 201010553455 A CN201010553455 A CN 201010553455A CN 102477102 A CN102477102 A CN 102477102A
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- bsp
- hemostatic material
- hemostatic
- bletilla striata
- applying
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Abstract
The invention relates to a method for applying bletilla striata polysaccharide to a hemostatic material. In the prior art, the hemostatic material is used by directly grinding bletilla tuber into powder, so that the method has the disadvantages of much impurity, incapability of controlling the effective content and inconvenience for use. According to the method disclosed by the invention, orchid, namely a dry bletilla tuber product, is dissolved and extracted by adding water and precipitated and dried by using ethanol to form coarse powder; the method has the advantages of non-complex extraction method, low cost, high effective content, less impurity and good hemostatic effect; after being processed, the coarse powder can be made into products, such as a sponge type material, a hemostatic tooth plug and the like; and the method is widely applied to medicine and biomedical engineering.
Description
Technical field
The invention belongs to field of medical materials, concrete relate to the method that a kind of BSP is used for hemostatic material.
Background technology
Used hemostatic material is directly the use of claying into power of orchid bletilla striata stem tuber dry product in the prior art, and its shortcoming is not add extraction, and foreign matter content is many; Working substance content can not be controlled; Use inconvenience,, still from bletilla striata stem tuber, extract the method for working substance polysaccharide up to today.
Summary of the invention
The present invention is directed to the deficiency that prior art exists, provide a kind of BSP to be used for the method for hemostatic material.
The object of the invention can reach through following measure: the process for extracting of hemostatic material BSP is with dry product bletilla striata stem tuber grinding and sieving; The solid that sieves is dissolved in deionized water, and by weight the solid that sieves: the deionized water ratio is 1: 5-6,70 ± 5 ℃ of temperature, dissolution time 2-3 hour; Centrifugal 10-15 minute, must clarify viscous solution; Flocculation, 95 % ethanol with 2 times of amounts of centrifugal gained solution adding liquor capacity leave standstill, and filter, and 50-60 ℃ is drying to obtain the BSP meal.
Extract the BSP meal of gained with the present invention, process the BSP aqueous solution of 2 %, through subzero treatment, vacuum-drying can be processed spongy material, processes hemostasis tooth plug with spongy material again, is widely used in medical science and biomedical engineering.
The present invention compares prior art and has following advantage: the BSP through extracting does not contain useless impurity, and hemostasis efficient is more excellent, and the clotting time shortens; Enhancing reduces the immunogenicity to tissue at the adhesion polarization and the pull-out capacity at bleeding tissue position, and this meal should be processed into various forms in addition; Like film, fiber, bar, rod and porous spongy thing; The hemostatic material that is used for clinical operation, the solid support material of slow releasing pharmaceutical promotes wound healing effect and anti-tumor function; Easy to use, have broad application prospects.
Embodiment
Lift an embodiment below, the present invention further specified:
Orchid bletilla striata stem tuber dry product is pulverized the back cross 20 mesh sieves, get 100 and restrain the solid that sieves, add 550 gram deionized waters, stir 72 ℃ of temperature; Dissolution time 2.5 hours, centrifugation, 3000 rev/mins of rotating speeds centrifugal 10-15 minute, are removed insolubles; Must clarify viscous solution, flocculation with centrifugal gained solution 100ml, adds the 95 % ethanol of 200ml, generates white flocks; Hold over night is filtered, and in 50-60 ℃ of drying, gets the BSP meal.
The BSP authentication method: sample thief meal 2g, add water 2Oml, hot dipping is 30 minutes on boiling water bath, filters, filtrates and carry out following experiment.
1, get filtrating 1ml, the alkaline cupric tartrate reagent 5-6 that adds new preparation drips, and heating is 5 minutes on boiling water dissolves, and produces the red-brown copper oxidule precipitation.
2, get filtrating 1ml, add 5 % α-3 of Cai phenol ethanol liquid and shake up, slowly add the 0.5ml vitriol oil, form purplish red colour circle at the interface place along test tube wall.
Claims (2)
1. a BSP is used for the method for hemostatic material, it is characterized in that dry product bletilla striata stem tuber grinding and sieving; The solid that sieves is dissolved in deionized water, and by weight the solid that sieves: the deionized water ratio is 1: 5-6,70 ± 5 ℃ of temperature, dissolution time 2-3 hour; Centrifugal 10-15 minute, must clarify viscous solution; Flocculation, 95 % ethanol with 2 times of amounts of centrifugal gained solution adding liquor capacity leave standstill, and filter, and 60 ℃ of dryings of 50-obtain the BSP meal.
2. a kind of BSP according to claim 1 is used for the method for hemostatic material; It is characterized in that BSP meal with the said extracted gained; Process the BSP aqueous solution of 2 %, through subzero treatment, vacuum-drying; Can process spongy material, process hemostatic material in medical use with spongy material again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105534558A CN102477102A (en) | 2010-11-22 | 2010-11-22 | Method for applying bletilla striata polysaccharide to hemostatic material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105534558A CN102477102A (en) | 2010-11-22 | 2010-11-22 | Method for applying bletilla striata polysaccharide to hemostatic material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102477102A true CN102477102A (en) | 2012-05-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105534558A Pending CN102477102A (en) | 2010-11-22 | 2010-11-22 | Method for applying bletilla striata polysaccharide to hemostatic material |
Country Status (1)
| Country | Link |
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| CN (1) | CN102477102A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104693247A (en) * | 2015-01-22 | 2015-06-10 | 武汉市第一医院 | Method for preparing Bletilla striata gelatin |
| CN114149610A (en) * | 2021-12-17 | 2022-03-08 | 四川大学 | Method for preparing hemostatic sponge by using oxidized bletilla striata modified collagen fibers |
-
2010
- 2010-11-22 CN CN2010105534558A patent/CN102477102A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104693247A (en) * | 2015-01-22 | 2015-06-10 | 武汉市第一医院 | Method for preparing Bletilla striata gelatin |
| CN114149610A (en) * | 2021-12-17 | 2022-03-08 | 四川大学 | Method for preparing hemostatic sponge by using oxidized bletilla striata modified collagen fibers |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120530 |