CN102465171A - Application of polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+>, and application method thereof - Google Patents
Application of polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+>, and application method thereof Download PDFInfo
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- CN102465171A CN102465171A CN2010105337085A CN201010533708A CN102465171A CN 102465171 A CN102465171 A CN 102465171A CN 2010105337085 A CN2010105337085 A CN 2010105337085A CN 201010533708 A CN201010533708 A CN 201010533708A CN 102465171 A CN102465171 A CN 102465171A
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Abstract
The invention relates to an application of a polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+>, wherein the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> can be adopted as a molecular recognition reagent for recognizing a G-quadruplex structure and an i-motif structure of telomere DNA. According to the present invention, the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> provides different bonding capacities for the G-quadruplex structure and the i-motif structure of the telomere DNA, and the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> provides better bonding capacity for the G-quadruplex structure of the telomere DNA compared to the i-motif structure of the telomere DNA, such that the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> can be adopted as the good molecular recognition reagent, wherein the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> is respectively added to the DNA solution containing the G-quadruplex structure and the DNA solution containing the i-motif structure, a fluorescence titration method or an ultraviolet titration method is adopted, the change of the fluorescence spectrum or the ultraviolet absorption spectrum is observed, and the two important structures of the telomere DNA can be effectively recognized and distinguished.
Description
Technical field
The present invention relates to metal complexes, be specifically related to a kind of many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+The application and methods for using them.
Background technology
In recent years; The research of many pyridines ruthenium complexe is very active, characteristics [1] such as this is that unique DNA binding ability, good excited state activity, anti-tumor activity, rigid plane, thermodynamic stability are good because these title complexs have, photochemistry and optical physics abundant information.These characteristics make this type title complex in numerous areas such as molecular biology, medical science, bio-inorganic chemistry, have important application prospects.Especially with aspects such as DNA bonding, as dna structure probe, dna molecular light open the light, aspect such as DNA footprint reagent, dna break reagent and cancer therapy drug has crucial application [2,3].
Generation 30 or 40 years 20th century; Hermann Muller and Barbara McClintock have proposed the notion of telomere simultaneously, and it is a kind of special knot hook of end of chromosome, can prevent chromosomal DNA degraded, terminal fusion, disappearance and improper reorganization; Keep chromosomal complete sum stable [3,4].Human telomere contain the double chain DNA sequence that repeats to be connected (5 '-TTAGGG): (5 '-CCCTAA).The chain of the rich G of karyomit(e) can be at K
+, Na
+Induce down formation to be made up of through the stable G-quadruplex structure of hydrogen bond the tetrad of piling up Deng positively charged ion, simultaneously, the rich C chain of its complementary can be based on C-C under acidity or neutrallty condition
+The base pair self-assembly forms so-called i-motif structure [5-10].G-quadruplex is considered to that Telomerase extended end grain in the body is had the negative regulation effect, and at present, G-quadruplex is considered to potential cancer therapy target.Compare G-quadruplex; The history of i-motif is also shorter; It relates to structure such as human telomere, centromere DNA and RNA insertion and finds some albumen mass-energy and rich C telomeric dna fragments specific bound energy forms the i-motif structure, and the biological importance of intramolecularly i-motif structure is proved.Except human telomere G-quadruplex, i-motif also is considered to regulate extremely attractive drug targets [11] for cancer chemotherapy and genetic transcription.The human genome sketch has promoted to explore the paces of special construction DNA.Because a large amount of G-quadruplex and i-motif structure in the human genome are proved, ensuing target is how to discern G-quadruplex and i-motif structure [12] through small molecules.Although Ru-polypyridine complex is many in the research aspect double-stranded DNA identification, the research of the effect situation of itself and special construction DNA is also fewer, especially to the rarely seen report in identification aspect of G-quadruplex and these two kinds of important structure of i-motif.
Summary of the invention
The object of the invention is exactly in order to provide a kind of many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Application and methods for using them as the molecular recognition reagent of discerning the telomeric dna structure.
In order to achieve the above object, the present invention has adopted following technical scheme: a kind of many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Application, said many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Have the structural formula shown in (1) formula as follows:
[Ru (bpy)
2(dppzi)]
2+In bpy represent dipyridyl, dppzi represents two pyridines [3,2-a:2 ', 3 '-c] and azophenlyene-11,12-imidazoles;
Described many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+As the G-qaudruplex structure of identification telomeric dna and the molecular recognition reagent of i-motif structure.
The telomeric dna that described telomeric dna is behaved.
The Nucleotide number of the DNA that the G-qaudruplex structure of described telomeric dna is used is 22, nucleotides sequence classifies 5 as '-AGGGTTAGGGTTAGGGTTAGGG-3 '.
The Nucleotide number of the DNA that the i-motif structure of described telomeric dna is used is 22, nucleotides sequence classifies 5 as '-CCCTAACCCTAACCCTAACCCT-3 '.
Above-mentioned many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+One of application method, be G-quadruplex structure and the i-motif structure that adopts fluorescence titrimetric method identification telomeric dna, this method specifically may further comprise the steps:
1) with many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Be dissolved in the buffered soln of PH=5.5, be mixed with the complex solution that concentration is 5uM;
2) get above-mentioned complex solution and place sample pool, use the 450nm excited by visible light, observe luminous situation;
3) with the DNA storing solution of microsyringe Dropwise 5 uL in the sample pool; Behind the mixing 5 minutes; Use the 450nm excited by visible light; Observe the luminous intensity variations of solution at 610nm place, the DNA storing solution of adding equal volume in the sample pool stops titration behind continuous four equal no changes of titration fluorescence intensity repeatedly;
4) according to the G-quadruplex structure and the i-motif structure of the variation of fluorescence intensity identification telomeric dna; Fluorescence intensity significantly strengthens the G-quadruplex structure that has telomeric dna in the expression DNA storing solution, and fluorescence intensity does not have the i-motif structure that noticeable change is represented there is not the G-quadruplex structure of telomeric dna in the DNA storing solution or had telomeric dna.
Above-mentioned many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Application method two, be G-quadruplex structure and the i-motif structure that adopts ultraviolet titration method identification telomeric dna, this method specifically may further comprise the steps:
1) with many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Be dissolved in the buffered soln of PH=5.5, be mixed with the complex solution that concentration is 10uM;
2) in the double beam system of uv-visible absorption spectra appearance, reference cell and sample pool are done system's zeroing, after the baseline stability; The buffered soln that in reference cell, adds 500uL PH=5.5 is done reference; In sample pool, add complex solution, with microsyringe each in reference cell and the sample pool DNA storing solution and the mixing 5 minutes of dropping equal volume, do not change until continuous 4 absorption spectrums; At this moment, the concentration of DNA storing solution in complex solution reaches capacity;
3) variation of the electronic absorption spectroscopy of detection title complex in the 200-800nm scope;
4) according to absorption spectrum lose lustre the G-quadruplex structure of rate situation identification telomeric dna and the i-motif structure of telomeric dna; Produce 19.3% lose lustre rate and 6nm red shift at the 431nm place and represent to exist in the DNA storing solution G-quadrulex structure of telomeric dna, in the expression DNA storing solution of rate and 3nm red shift is lost lustre in 431nm place generation 10.9%, have the i-motif structure of telomeric dna.
The buffered soln of described PH=5.5 is formulated by KCl and Tris or NaCl and Tris.
Because many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+G-quadruplex structure to telomeric dna has different binding abilities with the i-motif structure, promptly with respect to the i-motif structure, and many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+G-quadruplex there is better binding ability.Therefore, many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Can be used as good molecular recognition reagent.Through with many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Join respectively in the dna solution that contains these two kinds of structures, employing fluorometric titration or the titrating method of ultraviolet, the variation size of observing fluorescence, uv absorption spectrum can these two kinds important telomere structures of effective recognition and differentiation.
Description of drawings
Fig. 1, Fig. 2 are many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Strong and weak with the relative fluorescence of two kinds of structural DNA effects, wherein, Fig. 1 is many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Change with the fluorescence intensity of G-quadruplex effect, Fig. 2 is [Ru (bpy)
2(dppzi)]
2+Change with the fluorescence intensity of i-motif effect.
Fig. 3, Fig. 4 are many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+With the uv-absorbing spectrogram of two kinds of structural DNA effects, wherein, Fig. 3 is many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Change collection of illustrative plates with the uv-absorbing of G-quadruplex effect, Fig. 4 is [Ru (bpy)
2(dppzi)]
2+Change collection of illustrative plates with the uv-absorbing of i-motif effect.
Embodiment
One, the preparation of buffered soln
Prepare following buffered soln with high purity water:
Buffered soln 1:100mM KCl, 10mM Tris, pH=5.5;
Buffered soln 2:100mM NaCl, 10mM Tris, pH=5.5;
Two, the preparation of DNA storing solution
1) preparation of G-quadruplex DNA storing solution
Get the rich G single stranded DNA of certain mass (nucleotides sequence classify 5 as '-AGGGTTAGGGTTAGGGTTAGGG-3 '), be dissolved in buffered soln 1 or buffered soln 2, heated sealed to 90 ℃, and kept 5 minutes.Naturally cool to room temperature then, put into 4 ℃ of refrigerators and kept 24 hours, subsequent use.And with the absorbance A of ultraviolet spectrophotometer measurement DNA at 260nm
260Molar extinction coefficient is 2.285 * 10
5m
3Cm
-1, DNA concentration [DNA]=K * A
260/ 2.285 * 10
5(k is an extension rate), unit is mol L
-1
2) preparation of i-motifDNA storing solution
Get the C single stranded DNA (nucleotides sequence classify 5 as '-CCCTAACCCTAACCCTAACCCT-3 ') of certain mass, be dissolved in buffered soln 1 or buffered soln 2, put into 4 ℃ of refrigerators and kept 24 hours, subsequent use.And with the absorbance A of ultraviolet spectrophotometer measurement DNA at 260nm
260Molar extinction coefficient is 1.94 * 10
5m
3Cm
-1, DNA concentration [DNA]=K * A
260/ 1.94 * 10
5(k is an extension rate), unit is mol L
-1
Three, experimental section
1) fluorescence characterizes
With many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Be dissolved in buffered soln 1 or buffered soln 2, be mixed with the complex solution that concentration is 5uM.(like 450nm) excites above-mentioned complex solution with visible light, observes luminous situation.Add the DNA storing solution of 5uL in the sample pool with microsyringe, inhale repeatedly with liquid-transfering gun and tell mixing, after 5 minutes, be measured to solution and locate luminous intensity at 610nm (450nm excites) and increase.Add the DNA storing solution of equal volume repeatedly in the sample pool, observing fluorescence intensity increases gradually.Behind continuous 4 equal no changes of titration fluorescence intensity, stop titration.Fig. 2 has showed the relative intensity of fluorescence of title complex when G-quadruplex DNA and i-motifDNA occurring.Title complex has certain luminous in the aqueous solution.After adding G-four serobila DNA, fluorescence significantly strengthens; And after adding i-motif DNA, fluorescence does not almost change.Therefore, can whether strengthen and distinguish this two kinds of structures through observing fluorescence intensity.
2) uv-absorbing
With many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Be dissolved in buffered soln 1 or buffered soln 2, be mixed with the complex solution that concentration is 10uM.In the double beam system of uv-visible absorption spectra appearance, all add earlier solvent in reference cell and the sample pool and do system and return to zero.After the baseline stability; In reference cell, add 500uL buffered soln (potassium ion damping fluid) and do reference; Add complex solution in the sample pool, with microsyringe each in reference cell and the sample pool DNA storing solution of adding equal volume, behind the mixing 5min; The concentration ratio of DNA in complex solution is constantly increased, until saturated.In the 200-800nm scope, detect the variation of the electronic absorption spectroscopy of title complex.Spectrum change is as shown in Figure 3 in titration process.LC and MLCT peak produce tangible red shift and hypochromic effect between the uptake zone, obviously be better than i-motif DNA with the effect of G-quadruplex DNA effect.Add G-quadruplex to the red shift of the potassium ion buffered soln generation 6nm of title complex with in the hypochromic effect at 431nm place 19.3%.Add i-motif and only produce the red shift of 3nm and in the rate that loses lustre at 431nm place 10.9% to identical buffered soln.Isobestic point is positioned at 324nm and 475nm place.Lose lustre and red shift shows that intensive interacts between DNA base and title complex.Therefore, can distinguish two kinds of structures according to lose lustre rate and red shift situation of absorption spectrum.
Claims (7)
1. [the Ru (bpy) of pyridine ruthenium complexe more than a kind
2(dppzi)]
2+Application, said many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Have the structural formula shown in (1) formula as follows:
[Ru (bpy)
2(dppzi)]
2+In bpy represent dipyridyl, dppzi represents two pyridines [3,2-a:2 ', 3 '-c] and azophenlyene-11,12-imidazoles;
It is characterized in that: described many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+As the G-quadruplex structure of identification telomeric dna and the molecular recognition reagent of i-motif structure.
2. many pyridines ruthenium complexe as claimed in claim 1 [Ru (bpy)
2(dppzi)]
2+Application, be characterised in that: the telomeric dna that described telomeric dna is behaved.
3. many pyridines ruthenium complexe as claimed in claim 1 [Ru (bpy)
2(dppzi)]
2+Application, be characterised in that: the Nucleotide number of the DNA that the G-quadruplex structure of described telomeric dna is used is 22, nucleotides sequence classifies 5 as '-AGGGTTAGGGTTAGGGTTAGGG-3 '.
4. many pyridines ruthenium complexe as claimed in claim 1 [Ru (bpy)
2(dppzi)]
2+Application, be characterised in that: the Nucleotide number of the DNA that the i-motif structure of described telomeric dna is used is 22, nucleotides sequence classifies 5 as '-CCCTAACCCTAACCCTAACCCT-3 '.
5. many pyridines ruthenium complexe as claimed in claim 1 [Ru (bpy)
2(dppzi)]
2+Application method, it is characterized in that: adopt the G-quadruplex structure and the i-motif structure of fluorescence titrimetric method identification telomeric dna, this method specifically may further comprise the steps:
1) with many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Be dissolved in the buffered soln of PH=5.5, be mixed with the complex solution that concentration is 5uM;
2) get above-mentioned complex solution and place sample pool, use the 450nm excited by visible light, observe luminous situation;
3) with the DNA storing solution of microsyringe Dropwise 5 uL in the sample pool; Behind the mixing 5 minutes; Use the 450nm excited by visible light; Observe the luminous intensity variations of solution at 610nm place, the DNA storing solution of adding equal volume in the sample pool stops titration behind continuous 4 equal no changes of titration fluorescence intensity repeatedly;
4) according to the G-quadruplx structure and the i-motif structure of the variation of fluorescence intensity identification telomeric dna; Fluorescence intensity significantly strengthens the G-quadruplex structure that has telomeric dna in the expression DNA storing solution, and fluorescence intensity does not have the i-motif structure that noticeable change is represented there is not the G-quadruplex structure of telomeric dna in the DNA storing solution or had telomeric dna.
6. many pyridines ruthenium complexe as claimed in claim 1 [Ru (bpy)
2(dppzi)]
2+Application method, be characterised in that: adopt the G-quadruplx structure and the i-motif structure of ultraviolet titration method identification telomeric dna, this method specifically may further comprise the steps:
1) with many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Be dissolved in the buffered soln of PH=5.5, be mixed with the complex solution that concentration is 10uM;
2) in the double beam system of uv-visible absorption spectra appearance; Reference cell and sample pool are done system's zeroing; After the baseline stability, the buffered soln that in reference cell, adds 500uL PH=5.5 is done reference, in sample pool, adds complex solution; With microsyringe each in reference cell and the sample pool DNA storing solution and the mixing 5 minutes of dropping equal volume, behind continuous 4 titration absorbancy no changes, stop titration;
3) variation of the electronic absorption spectroscopy of detection title complex in the 200-800nm scope;
4) according to absorption spectrum lose lustre the G-quadruplex structure and the i-motif structure of rate and red shift situation identification telomeric dna; Produce 19.3% lose lustre rate and produce the G-quadruplex structure that has telomeric dna in the expression DNA storing solution of 6nm red shift at the 431nm place, produce 10.9% at 431nm place and lose lustre and have the i-motif structure of telomeric dna in the expression DNA storing solution of rate and generation 3nm red shift.
7. like claim 5 or 6 described many pyridines ruthenium complexe [Ru (bpy)
2(dppzi)]
2+Application method, be characterised in that: the buffered soln of described PH=5.5 is formulated by KCl and Tris or NaCl and Tris.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300936A (en) * | 2015-09-16 | 2016-02-03 | 北京师范大学 | Determination of yeast RNA (Ribonucleic Acid) by photoluminescence of ruthenium-based metal complex |
| CN109097449A (en) * | 2018-08-20 | 2018-12-28 | 陕西科技大学 | A kind of real-time fluorescence LAMP detection method and kit based on ruthenium complex |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289775A (en) * | 1999-09-24 | 2001-04-04 | 中国科学院化学研究所 | Ruthenium (II) polypyridine match and its preparing process |
-
2010
- 2010-11-05 CN CN2010105337085A patent/CN102465171A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289775A (en) * | 1999-09-24 | 2001-04-04 | 中国科学院化学研究所 | Ruthenium (II) polypyridine match and its preparing process |
Non-Patent Citations (3)
| Title |
|---|
| SHUO SHI等: "Interaction of [Ru(bpy)2(dppz)]2+ with human telomeric DNA: Preferential binding to G-quadruplexes over i-motif", 《BIOCHIMIE》 * |
| 曹秋娥等: "钌多吡啶配合物作为DNA结构探针的研究进展", 《云南化工》 * |
| 袁益娴等: "手性双核钌(II)配合物与DNA 的相互作用研究", 《无机化学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300936A (en) * | 2015-09-16 | 2016-02-03 | 北京师范大学 | Determination of yeast RNA (Ribonucleic Acid) by photoluminescence of ruthenium-based metal complex |
| CN109097449A (en) * | 2018-08-20 | 2018-12-28 | 陕西科技大学 | A kind of real-time fluorescence LAMP detection method and kit based on ruthenium complex |
| CN109097449B (en) * | 2018-08-20 | 2022-05-20 | 陕西科技大学 | Real-time fluorescence LAMP detection method and kit based on metal ruthenium complex |
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Application publication date: 20120523 |