CN102464437B - Method for treating beryllium-containing waste water by using kieselguhr-bacterium compound body - Google Patents
Method for treating beryllium-containing waste water by using kieselguhr-bacterium compound body Download PDFInfo
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- CN102464437B CN102464437B CN 201110320760 CN201110320760A CN102464437B CN 102464437 B CN102464437 B CN 102464437B CN 201110320760 CN201110320760 CN 201110320760 CN 201110320760 A CN201110320760 A CN 201110320760A CN 102464437 B CN102464437 B CN 102464437B
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Abstract
The invention discloses a method for treating beryllium-containing waste water by using a kieselguhr-bacterium compound body, which is especially applicable to the beryllium-containing waste water which is pretreated by a deep physical-chemical treatment method. The method comprises the following specific steps of: immobilizing bacteria by using modified kieselguhr to prepare the kieselguhr-bacterium compound body, adding 0.3 to 0.7 grams of kieselguhr-bacterium compound body into each liter of waste water which is pretreated by using the conventional physical-chemical method and has the beryllium content of below 200 ug/L according to the beryllium content in the waste water, aerating for 3 to 4 hours, standing and precipitating, separating sludge from water, and discharging the water which meets the national discharge standard. The method has the characteristics of high microorganism concentration, high selectivity, capability of purifying and keeping efficient bacteria, high reaction speed, less run-off cells, less produced sludge, good solid and liquid separation effect, wide pH value and temperature ranges, good treatment effect, stable discharged water meeting the standard, and the like.
Description
Technical field
The present invention is a kind of method that adopts treating beryllium-containing waste water by using kieselguhr-bacterium compound body, and particularly the advanced treatment of beryllium-containing wastewater belongs to field of waste water treatment in the environment protection.
Technical background
Beryllium is a kind of rare light metals, is widely used in the fields such as nuclear power, guided missile, satellite, spacecraft and aircraft.Beryllium-containing wastewater is mainly from the factory effluent of smelting, mining and special material, radio component parts and Meter Parts.Although beryllium content is little in the waste water, but beryllium and compound thereof all are Poisons, the toxicity less of metallic beryllium, after beryllium enters human body, the beryllium oxide of indissoluble can cause pneumonia, the beryllium compound of solubility can cause the pathology of internal organs or tissue and carcinogenic, and the beryllium speed that metabolism is gone out from tissue is slow, discharging that therefore must strict control beryllium-containing wastewater.
At present, relatively less to the Processing Technology Research of beryllium-containing wastewater both at home and abroad, research range is also narrow, and what generally adopt on engineering is used is physico-chemical process.Neutralization-flocculation sediment-sand filtration method can be down to beryllium 10 ~ 20 μ g/L, but waste water still difficulty reaches national sewage comprehensive emission standard value 5 μ g/L, need further to adopt micro porous pe pipe filtration, absorption or ion-exchange even depth to process, complex process, cost are high, water outlet difficulty up to standard.The lime Neutralization settlement is processed beryllium-containing wastewater, can make the beryllium content behind the sewage Neutralization settlement be down to 100 μ g/L.In recent years, biological process attracts people's sight gradually with its plurality of advantages, becomes the potential means that remove trace metal and reclaim precious metal from waste water, and will replace gradually conventional physico-chemical processes.External existing research all comes to be in the research and probe stage except anhydrating middle beryllium with single creature, is difficult to meet the need of market.Notification number is the method that CN101428931 discloses a kind for the treatment of beryllium-containing wastewater with biological agent, the method in the biotechnological formulation advanced treatment lime that makes with thiobacillus ferrooxidant, thiobacillus thiooxidans composite bacteria and after beryllium-containing wastewater, but the thalline physical strength is low, density is little, particle is thin, behind adsorbing metal, there is the solid-liquid separation difficulty.
The present invention utilizes diatomite to make the bacterium immobilization, overcome the deficiency of the aspects such as conventional physical chemical process complex process, fluctuation of service, water outlet difficulty up to standard, solved behind the microorganism adsorption metal that solid-liquid separation is difficult, the restricted difficulty of adsorption activity, a kind of new approaches of advanced treatment beryllium-containing wastewater are provided, have remarkable economic and social benefit, have broad application prospects.
Summary of the invention
The objective of the invention is for overcoming the deficiencies in the prior art, a kind of method of utilizing treating beryllium-containing waste water by using kieselguhr-bacterium compound body is provided, and it is high that the present invention has a microorganism concn, and selectivity is good, energy purifying and maintenance high-efficiency strain, be swift in response, cell runs off few, and sludge output is few, good effect of separating solid from liquid, the characteristics such as pH value and temperature range are wide, and treatment effect is good, and stable water outlet is up to standard.
The method for the treatment of beryllium-containing waste water by using kieselguhr-bacterium compound body may further comprise the steps:
(1) utilizes traditional physico-chemical process pre-treatment beryllium-containing wastewater
(2) according to beryllium content in the waste water, modification infusorial earth-bacterium complex body joins in the pretreated waste water with the dosage of every liter of waste water 0.3～0.7g, aeration 3～4 hours;
(3) staticly settle, mud-water separation, the upper strata stillness of night directly discharges or reuse, and the beryllium slag can be used as other industrial raw materials for production and sells.
Described diatomite modified method is:
(1) gets diatomite 55～100g, add the 250ml deionized water, with Ca (OH)
2/ HCl regulates soil sample pH7.0～8.0, and make up water is to 300ml, and ultrasonic wave is disperseed 45min, stirs suspension, and after 120 mesh sieves, oven drying (55 ℃～65 ℃) is put in sedimentation, crosses 100 mesh sieves;
(2) the diatomite 45g that takes by weighing after the processing joins in the 480ml sodium carbonate solution, slowly add while stirring saturated calcium chloride solution, filter after reaction finishes, three's solid-liquid mass ratio is 1:11:7, ore pulp repeatedly washs with deionized water, until till unnecessary chlorion cleans;
(3) 105 ℃ of lower oven dry place 250 ℃ of retort furnaces to toast 2h again, make modification infusorial earth.
The preparation method of the complex body of described diatomite-bacterium is:
(1) subtilis, pseudomonas, Arthrobacter are inoculated in the triangular flask that the 255ml liquid nutrient medium is housed, place constant-temperature table, after cultivating 10～14h under 150r/min, 22～35 ℃ of conditions, take out, with the centrifugal 15min of the rotating speed of 4000r/min, supernatant discarded night, thalline with sterilized water washing 3～4 times after, be suspended from pH7.0, in the TriS-HCl damping fluid of concentration 0.02mol/L;
(2) get modification infusorial earth 5～10g, at 125 ℃ of lower autoclaving 30min;
(3) will the go out modification infusorial earth of bacterium mixes with the composite bacterial bacteria suspension 50ml for preparing, place constant-temperature table, regulating temperature is 25～40 ℃, 150r/min fully vibrates and contacts 60min, leave standstill 30min, leach particulate matter, wash, remove the firmly thalline of absorption with physiological saline, make diatomite-bacterium complex body.
Embodiment
The present invention is described in further detail below in conjunction with example.
Example 1
The preparation modification infusorial earth:
Get diatomite 100g, add the 250ml deionized water, with Ca (OH)
2/ HCL regulates soil sample pH7.5, and make up water is to 300ml, and ultrasonic wave is disperseed 45min, stirs suspension, and after 120 mesh sieves, oven drying (55 ℃～65 ℃) is put in sedimentation, crosses 100 mesh sieves; The diatomite 45g that takes by weighing after the processing joins in the 480ml sodium carbonate solution, slowly adds while stirring saturated calcium chloride solution, filters after reaction finishes, and ore pulp repeatedly washs with deionized water, until till unnecessary chlorion cleans; 105 ℃ of lower oven dry, place again 250 ℃ of retort furnaces to toast 2h, make modification infusorial earth.
Example 2
The preparation of the complex body of diatomite-bacterium:
Subtilis, pseudomonas, Arthrobacter are inoculated in the triangular flask that the 250ml liquid nutrient medium is housed, place constant-temperature table, after cultivating 12h under 150r/min, the 30 ℃ of conditions, take out, with the centrifugal 15min of the rotating speed of 4000r/min, supernatant discarded night, thalline with sterilized water washing 3～4 times after, be suspended from pH7.0, in the TriS-HCI damping fluid of concentration 0.02mol/L; Get example 1 modification infusorial earth 5g, at 125 ℃ of lower autoclaving 30min; The modification infusorial earth of bacterium of will going out mixes with the composite bacterial bacteria suspension 50ml for preparing, place constant-temperature table, regulating temperature is 30 ℃, 150r/min fully vibrates and contacts 60min, leave standstill 30min, leach particulate matter, wash, remove the firmly thalline of absorption with physiological saline, make diatomite-bacterium complex body.
Example 3
With example 2 diatomite-bacterium complex body take the dosage of every liter of waste water 0.7g join after traditional physico-chemical process pre-treatment beryllium content in 200 μ g/L, pH=8.5, water temperature in 40 ℃ waste water, behind the aeration 4 hours, staticly settle, mud-water separation, waste water beryllium content has dropped to 4 μ g/L, is lower than national sewage comprehensive emission standard value 5 μ g/L.
Example 4
With example 2 diatomite-bacterium complex body take the dosage of every liter of waste water 0.6g join after traditional physico-chemical process pre-treatment beryllium content in 140 μ g/L, pH=10.0, water temperature in 35 ℃ waste water, behind the aeration 4 hours, staticly settle, mud-water separation, waste water beryllium content has dropped to 2 μ g/L.
Example 5
With example 2 diatomite-bacterium complex body take the dosage of every liter of waste water 0.5g join after traditional physico-chemical process pre-treatment beryllium content in 90 μ g/L, pH=9, water temperature in 25 ℃ waste water, behind the aeration 3 hours, staticly settle, mud-water separation, waste water beryllium content has dropped to 1 μ g/L.
Example 6
With example 2 diatomite-bacterium complex body take the dosage of every liter of waste water 0.4g join after traditional physico-chemical process pre-treatment beryllium content in 30 μ g/L, pH=8.5, water temperature in 20 ℃ waste water, behind the aeration 3 hours, staticly settle, mud-water separation, waste water beryllium content has dropped to 0.8 μ g/L.
Example 7
With example 2 diatomite-bacterium complex body take the dosage of every liter of waste water 0.3g join after traditional physico-chemical process pre-treatment beryllium content in 10 μ g/L, pH=7.5, water temperature in 30 ℃ waste water, behind the aeration 4 hours, staticly settle, mud-water separation, processed waste water does not detect beryllium.
Claims (1)
1. the method for the treatment of beryllium-containing waste water by using kieselguhr-bacterium compound body is characterized in that: utilize the diatomite after the modification to make the bacterium immobilization, make diatomite-bacterium complex body; Described diatomite modified method is: (1) gets diatomite 55～100g, adds the 250mL deionized water, with Ca (OH)
2/ HCI regulates soil sample pH7.0～8. and 0, make up water is to 300ml, and ultrasonic wave is disperseed 45min, stirs suspension, and after 120 mesh sieves, oven drying is put in sedimentation, and drying temperature is 55 ℃～65 ℃, crosses 100 mesh sieves; (2) the diatomite 45g that takes by weighing after the processing joins in the 480mL sodium carbonate solution, slowly add while stirring saturated calcium chloride solution, filter after reaction finishes, three's solid-liquid mass ratio is 1: 11: 7, ore pulp repeatedly washs with deionized water, until till unnecessary chlorion cleans; (3) 105 ℃ of lower oven dry, and place 250 ℃ of retort furnaces to toast 2h, make modification infusorial earth; Described diatomite-bacterium compound system Preparation Method is: (1) is inoculated into subtilis, pseudomonas, Arthrobacter in the triangular flask that the 255ml liquid nutrient medium is housed, place constant-temperature table, after cultivating 10～14h under 150r/min, 22～35 ℃ of conditions, take out, with the centrifugal 15min of the rotating speed of 4000r/min, abandoning supernatant is after thalline washs 3～4 times with sterilized water, be suspended from pH7.0, in the TriS-HCl damping fluid of concentration 0.02mol/L; (2) get modification infusorial earth 5～10g, at 125 ℃ of lower autoclaving 30min; (3) will the go out modification infusorial earth of bacterium mixes with the composite bacterial bacteria suspension 50ml for preparing, place constant-temperature table, regulating temperature is 25～40 ℃, 150r/min fully vibrates and contacts 60min, leave standstill 30min, leach particulate matter, wash, remove the firmly thalline of absorption with physiological saline, make diatomite-bacterium complex body.
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| CN105130011B (en) * | 2015-09-07 | 2017-12-22 | 深圳市德庆科技有限公司 | Diatomite sewage-treating agent, preparation method and its technology application |
| CN107937198B (en) * | 2018-01-08 | 2021-03-12 | 吉林华兰德酒庄有限公司 | Brewing method of ice wine suitable for hyperglycemia population |
| CN109319973B (en) * | 2018-10-09 | 2021-09-21 | 江钨世泰科钨品有限公司 | Recycling method for beryllium in P waste liquid measured by beryllium loading method |
| CN115672269A (en) * | 2022-09-30 | 2023-02-03 | 南华大学 | Calcium carbonate modified biochar, preparation method thereof and application thereof in treating beryllium-containing wastewater |
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| CN101676224A (en) * | 2008-09-18 | 2010-03-24 | 中国科学院福建物质结构研究所 | Method for treating industrial chromium-containing waste water by bacteria |
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| CN101676224A (en) * | 2008-09-18 | 2010-03-24 | 中国科学院福建物质结构研究所 | Method for treating industrial chromium-containing waste water by bacteria |
Non-Patent Citations (4)
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| 固定化微生物技术及其在重金属废水处理中的应用;徐雪芹 等;《环境污染治理技术与设备》;20060731;第7卷(第7期);第99-105页 * |
| 徐雪芹 等.固定化微生物技术及其在重金属废水处理中的应用.《环境污染治理技术与设备》.2006,第7卷(第7期),第99-105页. |
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