CN102441135A - Traditional Chinese medicine for treating lung cancer and stomach cancer, its preparation method and quality control technology - Google Patents
Traditional Chinese medicine for treating lung cancer and stomach cancer, its preparation method and quality control technology Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine for treating lung cancer and stomach cancer, its preparation method and quality control technology. The traditional Chinese medicine for treating lung cancer and stomach cancer can be prepared into particulate agents, capsules, tablets, and oral liquids. The preparation method comprises the following steps: taking 20 grams each of fagopyrum dibotrys, rhizoma sparganii, and rhizoma curcumae, and crushing into fine powder; adding water in the balance consisting of sophora flavescens and other seven kinds of Chinese medicines and decocting for 3 times, combining the decoction, filtering, standing for 24 h, taking the supernatant and condensing and cooling, adding equivalent alcohol unitl the alcohol content is 45-45 %, fully stirring, standing for 24 h, taking the supernatant and condensing into a stiff paste, mixing the stiff paste with fagopyrum dibotrys, rhizoma sparganii, and rhizoma curcumae fine powder, carrying out depression dry at a temperature of 80 DEG C, crushing into fine powder, adding proper amount of dextrin and cane sugar, uniformly mixing, pelleting, drying, and carrying out size stabilization to prepare into 1000 grams. The Chinese medicine can fundamentally control disease, has the advantages of little side effect, low occurring rate of adverse reaction, and definite therapeutic effect, and can be longer-term taken.
Description
Technical field
The present invention relates to the field of Chinese medicines, specifically is a kind of Chinese medicine of treating tumor disease pulmonary carcinoma, gastric cancer and preparation method thereof and Quality Control Technology.
Background technology
China's tumor patient has about 2,200,000 people at present, and its serious threat human beings'health becomes one of three big killers.The reason that causes tumor take place to increase has: exopathogenic factor---industrial pollution, air breakup, the combined effect of carcinogen such as exceed standard of food pesticide; Endogenous cause of ill---autoimmune system change and genetic sudden change etc. cause.Tumor is divided into optimum and pernicious, and this research is mainly the medicine of treatment malignant tumor.To the concern of malignant tumor much larger than optimum, because its disability rate, mortality rate are far above optimum.The sickness rate pro-is in China's malignant tumor: hepatocarcinoma, pulmonary carcinoma, gastrointestinal cancer etc.; These tumor treatment become the emphasis of current medical research; The mainly effective treatment of doctor trained in Western medicine is operation, radiation and chemotherapy, still has many side reactions, influences big shortcoming to the shortcoming of normal primary cellular defect with to patient's digestive system, blood system.And the advanced malignant tumor patient often loses the surgical engine meeting, because the whole body situation is poor, also is difficult to tolerate intensive chemotherapy, radiotherapy etc.; Or because chemotherapy repeatedly; Tumor cell produces drug resistance, and conventional chemotherapy difficulty again proves effective, and Chinese medicine becomes the important component part of malignant tumor Comprehensive Treatment.That the traditional Chinese medical science is thought is weakened body resistance, cancer is malicious, blood stasis and expectorant play an important role in tumor invasion with fixed attention.It is long-pending to think that cancer belongs to disease, how because of the pent-up of feelings will, and injury due to diet, the evil enemy intrusion or invasion of gas, positive QI-insufficiency, internal organs are become estranged, evil malicious internal resistance, QI-blood circulation is obstructed, due to stopping in the congestion.
Summary of the invention
The invention provides the Chinese medicine and preparation method thereof and the Quality Control Technology of a kind of tumor disease pulmonary carcinoma, gastric cancer, described Chinese medicine is control disease fundamentally, and side effect is little, adverse reaction rate is low, and determined curative effect, can take for a long time.
Technical scheme of the present invention is:
The understanding that motherland's medical science also early has malignant tumor; The name of disease record of " tumor " is just arranged in the inscriptions on bones or tortoise shells that is unearthed in Yin Dynasty ruins; " Ling Shu Miraculous Pivot or Divine Axis Ci Jiezhen evil a piece of writing " thinks that the cause of disease of tumor is " the evil residence therebetween, of a specified duration and interior ", and tentatively with tumor be divided into nodular varicosity, intestinal tumour, spine tumour, sarcoma and former times tumor etc.There is the medicine of anti-goiter the Classic of Mountains and Rivers beginning.The record of " Shanxi book " existing operative treatment.The Song dynasty Yang Shiying is pointed out " the core vegetarian directs at attaches 22 cancer side's opinions of something lost side's opinion ": " it is dark that cancer person goes up relative superiority or inferiority, the shape of cave, tired hanging down ... malicious root is hidden deeply, in perforation is passed through ...." these argumentations are very similar with the performance of malignant tumor.After the Yuan Dynasty, it is the basis of morbidity that the sage of the past recognizes weakened body resistance, invades with positive exogenous pathogen, and mechanism of qi is not declared, and expectorant blood is fought and had the pass; The Qing Dynasty has recognized that can not be in partial cut, moxa-moxibustion, acupuncture etc., in order to avoid impel the tumor diffusion.Write " surgery card is controlled the full piece of writing of giving birth to " like clear Wang Hongxu and " to avoid greatly and to operate on, to open that then magic flower is the most miserable." in traditional Chinese medical science ancient books, the common name of disease relevant with tumor has: breast carcinoma, choke separated, obstruction and rejection, the liver rump, gather, lump in the abdomen, goiter, carcinoma of the penis, indurated subcutaneous mass, furuncle of external auditory meatus etc.
Medicine of the present invention is made up of 12 flavor Chinese medicines such as Radix Sophorae Flavescentis, Rhizoma Fagopyri Dibotryis, Radix Sophorae Tonkinensis, Herba Scutellariae Barbatae, Rhizoma Curcumae, Radix Codonopsis, Radix Notoginseng.Wherein Radix Sophorae Flavescentis nature and flavor bitter cold is gone into liver, all warps of stomach, and heat-clearing and toxic substances removing is attacked knot and loose swollen." herbal classic ": " ... main stagnation of QI in chest and abdomen, lump in the abdomen, jaundice, drowning has the heeltap, relieves oedema or abdominal distension through diuresis or purgation, and it is swollen to remove pain." monarch drug in the side of being.Rhizoma Fagopyri Dibotryis, Radix Sophorae Tonkinensis, Herba Scutellariae Barbatae three flavors close monarch drug among heat-clearing and toxic substances removing, the congestion that looses, and eliminating mass is long-pending, has certain cancer antitumaous effect that presses down.The effect of Rhizoma Curcumae: " main gather all gas, for the medicine of spirit ".Wang Haogu is then said Rhizoma Curcumae: " logical Liver Channel gathers blood ", " blood in the dispelling the stagnated QI is gone into the gas medicine and is sent out all perfume, and cloud is a purgative prescription, also can QI invigorating." lid is invigorated blood circulation and must be regulated the flow of vital energy earlier, so the blood stasis stagnation of QI, the disease of all pains is commonly used effective, and does not have and complete brokenly hemorrhage worry.Make in the side with Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, sweet flat priming is gone into spleen, and the ability replenishing QI to invigorate the spleen consolidates eliminating evil.The all medicines of we share hardship and are cold, attack long-pending and just do not hinder, and play the effect of heat-clearing and toxic substances removing, activating blood circulation to dissipate blood stasis, resolving mass and swelling altogether.
Medicine composition of the present invention can be made into granule, capsule, tablet, oral liquid.
Figure of description
Fig. 1 is the moisture absorption percentage rate
Fig. 2 is a critical relative humidity
Specific embodiment granule (the bitter gold grain of compound recipe)
1. prescription is formed
Radix Sophorae Flavescentis 800g Rhizoma Fagopyri Dibotryis 600 Radix Sophorae Tonkinensis 500g Herba Scutellariae Barbatae 500g
The triangular 500g Rhizoma Curcumae of Radix Codonopsis 500g 500g Radix Notoginseng 400g
Spica Prunellae 400g Sargassum 400g Rhizoma Atractylodis Macrocephalae 200g Radix Glycyrrhizae 200g
Process 1000g
2. preparation technology
More than 12 the flavor, depletion Semen Fagopyri Esculenti, triangular, each 20g of Rhizoma Curcumae are ground into fine powder; All the other Radix Sophorae Flavescentiss etc. eight flavor decocte with water three times, the 1st 8 times of amount, 2 hours, the second time 6 times of amounts, 1.5 hours, the 3rd 6 times of amount, 1 hour, collecting decoction; Filter, left standstill 24 hours, getting supernatant concentration to relative density is 1.10 (50 ℃～60 ℃), puts coldly, and adding equivalent ethanol is 45～50% to containing the alcohol amount; Fully stir, left standstill 24 hours, get supernatant, be condensed into thick paste, mix with Rhizoma Fagopyri Dibotryis, triangular, Rhizoma Curcumae fine powder; 80 ℃ of drying under reduced pressure are ground into fine powder, add dextrin 37g, sucrose 563g, and mixing is granulated; Drying, granulate is processed 1000g, promptly gets.
3. relevant research
3.1 solvent (water) extracts multiple
(1) water absorption rate: take by weighing 3 parts of Radix Sophorae Flavescentis 16g, Rhizoma Fagopyri Dibotryis 12g, Radix Sophorae Tonkinensis 10g, Herba Scutellariae Barbatae 10g, Radix Codonopsis 10g, triangular 10g, Rhizoma Curcumae 10g, Radix Notoginseng 8g, Spica Prunellae 8g, Sargassum 8g, Rhizoma Atractylodis Macrocephalae 4g, Radix Glycyrrhizae 4g; Respectively add 300ml water; Soak and after 30 minutes unnecessary unabsorbed water is poured out, calculate water absorption rate.The result sees table 2.
Table 2 water absorption rate result
The result shows: the water absorption rate of medical material is 189%, so medical material can be added 2 times of water gagings when extracting the 1st time.
(2) instrument and experiment material
Tianjin, island CS-900 type thin-layer chromatogram scanner (day island proper Tianjin company); The efficient lamellae of silica gel G (Haiyang Chemical Plant, Qingdao); The semi-automatic point sample instrument of CAMA Linomat IV.
The mensuration of (3) evaluation index---paste-forming rate
Take by weighing Radix Sophorae Flavescentis 16g, Rhizoma Fagopyri Dibotryis 12g, Radix Sophorae Tonkinensis 10g, Herba Scutellariae Barbatae 10g, Radix Codonopsis 10g, triangular 10g, Rhizoma Curcumae 10g, Radix Notoginseng 8g, Spica Prunellae 8g, Sargassum 8g, Rhizoma Atractylodis Macrocephalae 4g, Radix Glycyrrhizae 4g, extract merge extractive liquid, with different solvents shown in the factor level table; Filter, put and be concentrated into dry extract in the water-bath, put drying baker again in 105 ℃ of dryings 3 hours; Take out, insert in the exsiccator and cooled off 30 minutes, take out; Weigh rapidly, calculate.
The mensuration of (4) evaluation index---matrine content
Measure according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B)
1. the preparation of reference substance solution
Get the matrine reference substance, add ethanol and process the solution that every 1ml contains 1mg, as reference substance solution.
2. the preparation of need testing solution
Get the about 0.5g of dry extract, the accurate title, decide, and adds water 10ml, and jolting makes dissolving, adds ethanol 40ml again, jolting; Leave standstill, filter, filtrating evaporate to dryness, residue add 0.2% hydrochloric acid solution 20ml dissolving, filter, and filtrating adds strong ammonia solution 5ml; Extract 3 times with the chloroform jolting, each 10ml, the extracting solution of combined chloroform, evaporate to dryness, residue makes dissolving with ethanol 5ml gradation; And quantitatively be transferred in the 5ml measuring bottle, adding ethanol to scale, close plug shakes up, as need testing solution.
3. algoscopy
Draw above-mentioned need testing solution 2 μ l, reference substance solution 2 μ l and 4 μ l put respectively on same silica gel g thin-layer plate; (20: 20: 2: 1) be developing solvent, expansion was taken out with toluene-acetone-ethanol-strong ammonia solution; Dry, with improveing bismuth potassium iodide: the colour developing of IKI (1: 1) solution, scan according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B thin layer chromatography scanning); Wavelength X s=505nm, λ
R=650nm measures test sample trap integrated value and reference substance trap integrated value, calculates, and promptly gets.
(5) the extraction solvent multiple is investigated
We select three levels; Promptly add 6,8,10 times of water gagings and carry out optimal process; Take by weighing respectively 3 parts of Radix Sophorae Flavescentis Radix Sophorae Flavescentis 16g, Rhizoma Fagopyri Dibotryis 12g, Radix Sophorae Tonkinensis 10g, Herba Scutellariae Barbatae 10g, Radix Codonopsis 10g, triangular 10g, Rhizoma Curcumae 10g, Radix Notoginseng 8g, Spica Prunellae 8g, Sargassum 8g, Rhizoma Atractylodis Macrocephalae 4g, Radix Glycyrrhizae 4g; To measure matrine content and two indexs of paste-forming rate as its evaluation index, table 3 is seen in the factor level arrangement
Table 3 solvent load experimental factor water-glass
Each extraction scheme of pressing the design of factor level table of testing is operated, and measures paste-forming rate and matrine content respectively down with above-mentioned " evaluation index " item, tests 2 parts of operation repetitives at every turn, and every index employing weighted scoring method of result of the test is analyzed.Be decided to be 50 fens to two best indexs respectively, comprehensive grading is 100 minutes, weighted scoring under unified standard, paste-forming rate Y
1=Yi ÷ 6.08 * 50, matrine content Y
2=Yi ÷ 13.23 * 50 carries out comprehensively every index result, gets comprehensive grading=Y
1+ Y
2, the result sees table 4:
Table 4 solvent load test card (n=2)
The result shows: add the basic indifference of comprehensive grading of 6,8,10 times of amount solvents, so from producing actual investigation, select to add 6 times of water gagings and fry in shallow oil each 2 hours 3 times.Consider that the medical material water absorption rate is 189%, so add 8 times of amounts of water first, second and add water for the third time and be 6 times of amounts.
Demonstration test: by 1/10th amounts of prescription, extract 8 times of amounts for the first time, 2 hours respectively by above-mentioned The selection result decocte with water; 6 times of amounts for the second time, 1.5 hours, 6 times of amounts for the third time, 1 hour; Operation system in accordance with the law gets dry extract, and measures paste-forming rate and matrine content and carries out comprehensive grading, and the result sees table 5:
Table 5 demonstration test result
The result shows: the result of demonstration test meets with the extraction solvent multiple of screening, explains that this process stabilizing is feasible.
12.5.2.3 it is dry
Because the sample drying time that constant pressure and dry obtains is long; And be prone to harden, so this kind adopts drying under reduced pressure, get thick paste and the Rhizoma Fagopyri Dibotryis of extraction, triangular and Rhizoma Curcumae fine powder mixing after; Be tiled on the porcelain dish; The about 1cm of thickness is respectively at 60 ℃, 80 ℃, 100 ℃ drying under reduced pressure, with drying time, matrine content, whether breeze is arranged is that index is investigated.The result sees table 6:
Table 6 drying test result
The result shows: though 100 ℃ of drying times are short, have breeze to occur, 60 ℃ of drying times are oversize, so select 80 ℃ of drying processes that drying under reduced pressure is these article.
12.5.3 the selection of preparations shaping prescription
According to the extraction process of medical material, extract the medical material of a recipe quantity, get thick paste and Rhizoma Fagopyri Dibotryis, triangular, Rhizoma Curcumae fine powder mixing; 80 ℃ of drying under reduced pressure are ground into fine powder, add different auxiliary materials respectively by table 7 designed scheme, and mixing 16 mesh sieves are granulated; Dry; With melting, granularity (can not through 10 mesh sieves and the percentage rate that can account for total particle quantity through granule and the fine powder summation of 60 mesh sieves), the molding difficulty or ease be the investigation index, and the result sees table 7.
The screening of table 7 molding prescription
The result shows: melting, the granularity of prescription 3 all meet the granule requirement, and easy-formation, so select prescription 3.
12.5.4 the demonstration test of moulding process and detection data
Get Radix Sophorae Flavescentis, Rhizoma Fagopyri Dibotryis, Radix Sophorae Tonkinensis, Herba Scutellariae Barbatae, Radix Codonopsis, triangular, Rhizoma Curcumae, Radix Notoginseng, Spica Prunellae, Sargassum, the Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, by preparation technology's preparation.The result sees table 8, table 9.
Table 8 granule formation technology demonstration test
Table 9 granule testing data
12.5.5 particulate flowability and hygroscopicity are investigated
12.5.5.1 measure angle of repose
Adopt the fixed funnel method; With the series connection of 3 funnels and be fixed in the height place of 3cm on the coordinate of horizontal positioned; Carefully granule is poured into by hopper walls in the funnel of going up most, till the granule cone tip that forms on the graph paper touches funnel, surveyed the diameter (2R) of conical base by graph paper; (tg α=H/R), the result sees table 10 to calculate angle of repose.
Table 10 is measured angle of repose
The result shows: be 30.42 ° average angle of repose, shows that this particulate flowability is better.
12.5.5.2 the percentile mensuration of granule moisture absorption
The glass exsiccator that the sodium chloride supersaturated solution is contained in the bottom is put into 25 ℃ constant incubator constant weight 24 hours, and the relative humidity in this moment exsiccator is 75%.The particle drying for preparing to constant weight, is put into the granule of thick about 2mm in the weighing botle bottom of constant weight, put into above-mentioned glass exsiccator (the weighing bottle cap is beaten) in 25 ℃ of preservations, regularly (6,12,24,48,72 hours) weighing is calculated as follows hydroscopicity.
Table 11 granule moisture absorption percentage rate
The result shows: the prolongation in time of particulate moisture absorption percentage rate is not proportional increase, and the moisture absorption percentage rate tends towards stability gradually.
12.5.3 the measurement of granule critical relative humidity (CRH)
With the particle drying for preparing to constant weight; Put into the granule of thick about 2mm in the weighing botle bottom of constant weight; Accurately weighing is placed on the exsiccator interior (the weighing bottle cap is opened) of the supersaturated solution that fills 5 kinds of variable concentrations sulphuric acid and different salt respectively shown in the table 12; In 25 ℃ of constant incubators, keep weighing after 7 days, calculate the moisture absorption percentage rate.
Table 12 granule moisture absorption percentage rate
The corresponding abscissa of the point of intersection of tangents of doing Fig. 2 curve two ends is a critical relative humidity.This particulate critical relative humidity (CRH) is about 52%.During the packing granule, can be controlled at below 52% in big the production, make the water content of product too high and influence quality to avoid moisture absorption with reference to the humidity of this value with the workshop.
12.6 middle trial production
Preparation method of granules by above-mentioned definite technology is carried out pilot-scale production, and concrete data are seen table 13, table 14.
Table 13 granule pilot plant test data
Table 14 granule pilot scale testing data
Quality Control Technology
[character] these article are pale brown color to tan granule; Feeble QI is fragrant, sweet, little hardship of distinguishing the flavor of.
These article 40g is got in [discriminating] (1), and porphyrize is put in the tool plug conical flask, adds the moistening medicated powder of strong ammonia solution 10ml, adds chloroform 100ml, and supersound extraction 30 minutes filters, and filtrating is put evaporate to dryness in the water-bath, and residue adds ethanol 2ml makes dissolving, as need testing solution.Other gets matrine, tetrandrine, sophoridine reference substance, adds ethanol respectively and processes every 1ml and contain the solution that matrine, tetrandrine 1mg and every 1ml contain sophoridine 0.5mg, as reference substance solution.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B); Draw each 10 μ l of above-mentioned four kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform-acetone-methanol-strong ammonia solution (20: 2: 2: 0.1) be developing solvent; Launch; Take out, dry, spray is with rare bismuth potassium iodide test solution.In the test sample chromatograph, with matrine, tetrandrine, the corresponding position of sophoridine reference substance chromatograph on, show the speckle of same color.
(2) get Rhizoma Curcumae control medicinal material 1g, add water 20ml, flooded jolting 1 hour; Process suspension, the 20ml that adds diethyl ether, supersound extraction 5 minutes leaves standstill; Obtain ether layer, put evaporate to dryness in 50～60 ℃ of water-baths, residue adds ethyl acetate 2ml makes dissolving, as control medicinal material solution.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw and differentiate (2) item need testing solution, each 10 μ l of control medicinal material solution down, put respectively on same silica gel g thin-layer plate; With petroleum ether (30～60 ℃)-ethyl acetate (17: 3) is developing solvent; Launch, take out, dry; Spray is with 5% vanillin concentrated sulfuric acid solution, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with control medicinal material chromatograph Rf value 0.5～0.8 scope in the relevant position on, show the principal spot of same color.
(3) get these article 40g, porphyrize is put in the round-bottomed flask, adds ethyl acetate 100ml reflux, extract, 1 hour, filters, and filtrating discards.Filtering residue adds methanol 100ml reflux, extract, 1 hour, filters, and filtrating is put evaporate to dryness in the water-bath; The sodium hydroxide solution 30ml that residue adds 0.1mol/L makes dissolving, and the jolting that adds diethyl ether is extracted 3 times, each 15ml; Discard ether solution, the alkali liquor layer adds dilute hydrochloric acid and regulates pH value to 2, and the jolting that adds diethyl ether is extracted 3 times; Each 15ml, the acid solution layer is retained.Merge ether extracted liquid, put evaporate to dryness in the water-bath, residue adds dehydrated alcohol 2ml makes dissolving, as need testing solution.Other gets Spica Prunellae control medicinal material 2g, adds water 20ml and decocts 2 hours, filters, and filtrating adds the sodium hydroxide solution of 10mol/L and regulates pH value greater than 9, processes control medicinal material solution from " jolting that adds diethyl ether is extracted 3 times " with the need testing solution method for preparing.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With chloroform-acetone-formic acid (2.5: 1: 0.4) is developing solvent; Launch, take out, dry; Spray is with 1% ferric chloride ethanol test solution, and it is clear to be heated to the speckle colour developing in 100 ℃.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(4) get discriminating (3) acid solution layer down, add the ethyl acetate jolting and extract 3 times, each 15ml, merging ethyl acetate extraction liquid adds the saturated water washing of ethyl acetate 2 times; Each 15ml discards water lotion, and ethyl acetate liquid is put evaporate to dryness in the water-bath, and residue adds methanol and dissolves in right amount, adds an amount of polyamide fine powder and in water-bath, mixes appearance; Fling to methanol, be added on polyamide column (φ 1.8cm * 40cm, 3g, 40～60 orders; Dry column-packing) on,, discards 20% ethanol elution, continue with 70% ethanol 100ml eluting earlier with 20% ethanol 100ml eluting; Collect 70% ethanol elution, put evaporate to dryness in the water-bath, residue adds methanol 2ml makes dissolving, as need testing solution.Other gets Herba Portulacae Grandiflorae control medicinal material 2g, adds water 20ml and decocts 2 hours, filters, and filtrating adds the sodium hydroxide solution of 10mol/L and regulates pH value greater than 9, processes control medicinal material solution from " jolting that adds diethyl ether is extracted 3 times " with the need testing solution method for preparing.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on the silica gel g thin-layer plate of same usefulness 1% sodium hydroxide solution preparation; (4: 3: 1: 1) be developing solvent, expansion was taken out with ethyl acetate-butanone-formic acid-water; Dry; Spray, is put under the ultra-violet lamp (365nm) and is inspected in about 5 minutes of 80 ℃ of heating with 1% aluminum chloride alcoholic solution.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.
[inspection] should meet each item regulation (appendix IC of Chinese Pharmacopoeia version in 2005) relevant under the granule item.
[assay] got these article, and porphyrize is got about 2.0g, and accurate the title decides, and adds water 10ml, and jolting makes dissolving; Add ethanol 40ml again, jolting is left standstill, and filters, and filtrating evaporate to dryness, residue add 0.2% hydrochloric acid solution 20ml dissolving; Filter, filtrating adds strong ammonia solution 5ml, extracts 3 times with the chloroform jolting, each 10ml, combined chloroform extracting solution, evaporate to dryness; Residue makes dissolving with ethanol 5ml gradation, and quantitatively is transferred in the 5ml measuring bottle, adds ethanol to scale, and close plug shakes up, as need testing solution.Other gets the matrine reference substance, adds ethanol and processes the solution that every 1ml contains 1mg, as reference substance solution.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw above-mentioned need testing solution 2 μ l, reference substance solution 2 μ l and 4 μ l; Put respectively on same silica gel g thin-layer plate, with toluene-acetone-ethanol-strong ammonia solution (20: 20: 2: 1) be developing solvent, launch; Take out, dry, spray is to improve bismuth potassium iodide: IKI (1: 1) solution; On lamellae, cover onesize glass plate, use immobilization with adhesive tape on every side, carry out serration type scanning according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B thin layer chromatography scanning); Wavelength X s=505nm, λ
R=650nm measures test sample trap integrated value and reference substance trap integrated value, calculates, and promptly gets.
These article contain Radix Sophorae Flavescentis with matrine (C for every bag
15H
24N
2O) meter, must not be less than 33.0mg.
[function with cure mainly] heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, hard masses softening and resolving.Be used for by stagnation of QI-blood, pyretic toxicity internal resistance and the medium and advanced lung cancer, the patients with gastric cancer that cause.
[usage and consumption] is oral, one time 1 bag, 3 times on the one.
[specification] every packed 12g
[storage] sealing.
Treatment middle and advanced stage tumor
Under the present medical level of China, still there is the patient of considerable part can not in time find in time to treat cancer, so that loses the surgical engine meeting; Even be difficult to tolerate chemotherapy radiotherapy, though or the underwent operative chemotherapy radiotherapy, right unsatisfactory curative effect; Or to middle and advanced stage, and can only carry out some palliative therapy.This type patient and household's expected value greatly reduces, and eliminates cancerous protuberance, and the purpose of radical cure cancer is abandoned basically, and the person of asking only is with tumor existence, palliates the agonizing sufferings, and prolongs the generation phase.And this type of patient just is being fit to Chinese medicine, and the traditional Chinese medical science can actively be brought into play speciality in to this type of patient's treatment, transfer the leading role to by the supporting role.And the fact is verified, integrally-regulated through the traditional Chinese medical science, and how determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs can satisfy patient and appearance miracle that the household asked even had to a certain extent.Treat 198 routine middle and advanced stage clinical tumor observation of curative effect with the bitter gold grain of compound recipe, in patient's 198 examples, male's 144 examples, women's 54 examples, each age group all has, and meets the clinical diagnosis standard, and histopathology and/or cytodiagnosis are all arranged.The sick kind has pulmonary carcinoma, gastric cancer, breast carcinoma, malignant lymphoma etc.Be divided into two groups, single with the bitter gold grain group of compound recipe, for can not tolerating chemotherapy, refusal, whole body situation difference accepts chemotherapy or chemotherapy effect gastrointestinal disease patient repeatedly, capablely put at least 1 month before taking medicine, chemotherapy, totally 72 examples.The therapeutic alliance group, promptly the bitter gold grain of compound recipe adds conventional chemotherapy, totally 126 examples.Treatment is with the bitter gold grain of compound recipe, and 1 bag, every day three times, serveing on one month was 1 course of treatment.Matched group 1 week before chemotherapy begins to take.Curative effect determinate standard is divided into several, and each result is: (1) clinical symptoms, and take a turn for the better and to be more common in weak, poor appetite, night sweat alleviates or disappears, secondly be that pain relief, jaundice disappear, abdominal distention improvement etc., single medicine group improvement rate 57.3%; Combined group improvement rate 73.6%.(2) treatment back quality of life scoring, single medicine group improvement rate 56.9%, average KPS scoring improved 8.1 fens; Combined group improvement rate 61.1%, average KPS improved 4.4 fens.(3) short term effect list medicine group effective percentage 8.3%, combined group effective percentage 28.6%.Taking a turn for the better also appears in other indexs.
Claims (4)
1. treat pulmonary carcinoma, gastric cancer Chinese medicine for one kind, it is characterized in that processing by following Chinese medicine raw materials by weight proportion:
Radix Sophorae Flavescentis 700～900g Rhizoma Fagopyri Dibotryis 500～700g Radix Sophorae Tonkinensis 400～600g
Triangular 400～the 600g of Herba Scutellariae Barbatae 400～600g Radix Codonopsis 400～600g
Rhizoma Curcumae 400～600g Radix Notoginseng 300～500g Spica Prunellae 300～500g
Sargassum 300～500g Rhizoma Atractylodis Macrocephalae 100～300g Radix Glycyrrhizae 100～300g
2. the Chinese medicine of treatment pulmonary carcinoma according to claim 1, gastric cancer is characterized in that the weight proportion of its each component is:
Radix Sophorae Flavescentis 750～850g Rhizoma Fagopyri Dibotryis 550～650g Radix Sophorae Tonkinensis 450～550g
Triangular 450～the 550g of Herba Scutellariae Barbatae 450～550g Radix Codonopsis 450～550g
Rhizoma Curcumae 450～550g Radix Notoginseng 350～450g Spica Prunellae 350～450g
Sargassum 350～450g Rhizoma Atractylodis Macrocephalae 150～250g Radix Glycyrrhizae 150～250g
3. according to the Chinese medicine of claim 1 described treatment pulmonary carcinoma, gastric cancer, it is characterized in that the weight proportion of its each component is:
Radix Sophorae Flavescentis 800g Rhizoma Fagopyri Dibotryis 600 Radix Sophorae Tonkinensis 500g Herba Scutellariae Barbatae 500g
The triangular 500g Rhizoma Curcumae of Radix Codonopsis 500g 500g Radix Notoginseng 400g
Spica Prunellae 400g Sargassum 400g Rhizoma Atractylodis Macrocephalae 200g Radix Glycyrrhizae 200g
4. the preparation method of Chinese medicine of treatment pulmonary carcinoma according to claim 1, gastric cancer is characterized in that depletion Semen Fagopyri Esculenti, triangular, each 20g of Rhizoma Curcumae, is ground into fine powder; All the other Radix Sophorae Flavescentiss etc. eight flavor decocte with water three times, the 1st 8 times of amount, 2 hours, the second time 6 times of amounts, 1.5 hours, the 3rd 6 times of amount, 1 hour, collecting decoction; Filter, left standstill 24 hours, getting supernatant concentration to relative density is 1.10 (50 ℃-60 ℃), puts coldly, adds equivalent ethanol and is 45-50% to containing the alcohol amount; Fully stir, left standstill 24 hours, get supernatant, be condensed into thick paste, mix with Rhizoma Fagopyri Dibotryis, triangular, Rhizoma Curcumae fine powder; 80 ℃ of drying under reduced pressure are ground into fine powder, add dextrin 37g, sucrose 563g, and mixing is granulated; Drying, granulate is processed 1000g, promptly gets.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727854A (en) * | 2012-07-05 | 2012-10-17 | 许瑞琴 | Traditional Chinese medicine for treatment of gastric cancer |
| CN104887794A (en) * | 2015-05-22 | 2015-09-09 | 陈彬 | Traditional Chinese medicine composition for treating qi-stagnancy and blood-stasis type lung cancer |
| CN113663029A (en) * | 2021-08-30 | 2021-11-19 | 黄传贵 | Compound wild buckwheat rhizome composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101049484A (en) * | 2007-05-14 | 2007-10-10 | 郝来勤 | Strong effective Chinese traditional medicine for treating lung cancer and other various cancers |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101049484A (en) * | 2007-05-14 | 2007-10-10 | 郝来勤 | Strong effective Chinese traditional medicine for treating lung cancer and other various cancers |
Non-Patent Citations (1)
| Title |
|---|
| 刘福丹等: "FR/MA对HepG2肝癌细胞侵袭能力的影响", 《实用肿瘤杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727854A (en) * | 2012-07-05 | 2012-10-17 | 许瑞琴 | Traditional Chinese medicine for treatment of gastric cancer |
| CN102727854B (en) * | 2012-07-05 | 2013-08-28 | 许瑞琴 | Traditional Chinese medicine for treatment of gastric cancer |
| CN104887794A (en) * | 2015-05-22 | 2015-09-09 | 陈彬 | Traditional Chinese medicine composition for treating qi-stagnancy and blood-stasis type lung cancer |
| CN113663029A (en) * | 2021-08-30 | 2021-11-19 | 黄传贵 | Compound wild buckwheat rhizome composition |
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Application publication date: 20120509 |