CN102437361A - Microbial fuel cell reactor for chlorella culture - Google Patents

Microbial fuel cell reactor for chlorella culture Download PDF

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CN102437361A
CN102437361A CN2011103748237A CN201110374823A CN102437361A CN 102437361 A CN102437361 A CN 102437361A CN 2011103748237 A CN2011103748237 A CN 2011103748237A CN 201110374823 A CN201110374823 A CN 201110374823A CN 102437361 A CN102437361 A CN 102437361A
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chlorella
anode
water body
fuel cell
negative electrode
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CN102437361B (en
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周楚新
宋天顺
吴夏芫
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Nanjing Tech University
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Nanjing Tech University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/30Hydrogen technology
    • Y02E60/50Fuel cells
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P70/00Climate change mitigation technologies in the production process for final industrial or consumer products
    • Y02P70/50Manufacturing or production processes characterised by the final manufactured product

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fuel Cell (AREA)

Abstract

The invention relates to a microbial fuel cell reactor for chlorella culture, belonging to the technical field of environmental engineering. The reactor of the invention mainly comprises a reaction chamber containing water body sediments, a cathode, an anode, a lead and a resistor; the reaction chamber is a container with an open top and other closed surfaces, and a water body in the reaction chamber is layered into a sediment layer positioned at the lower part of the reaction chamber and a water body layer positioned at the upper part of the reaction chamber; the cathode is arranged in the water body layer, the anode is arranged in the sediment layer, the cathode, the anode and the resistor are connected in series through the conducting wires, and the resistor is arranged between the cathode and the anode. The device can oxidize organic matters in water sediments and can be used for manufacturing chlorella serving as a basic material for producing biodiesel at the same time, thereby achieving the double effects of energy and biological resource production and having higher social value and economic value.

Description

Be used for chlorella cultured microorganism fuel cell reaction device
Technical field
The present invention relates to a kind of chlorella cultured microorganism fuel cell reaction device that is used for, belong to field of environment engineering technology.
Background technology
Along with expanding economy, the progress of society and the continuous growth of population, human increasing to demands for energy, global energy crisis is outstanding day by day.Chlorella is a kind of photosynthetic efficiently plant, is rich in grease in its body, can the production biodiesel.Therefore prior art has related to the method for various cultivation chlorellas, and wherein providing of carbon dioxide is a key technology.Special in the open type reactor of highdensity cultivation of algae, low carbon dioxide content causes low chlorella growth speed, and how efficient the therefore cheap carbon dioxide that provides be considered to one of key technology that chlorella cultivates.
At present, organic (bed mud) content of water body deposit obviously raises, and its reason is the raising of waters primary productivity on the one hand, has caused endogenous organic input to roll up; Be that a large amount of organic substances enters into water body through the direct discharging of industrial wastewater and sanitary sewage and soil percolation, rainwash and the atmospheric sedimentation of agricultural chemicals on the other hand, quickened the enrichment of organic matter in the lake sediment.And organic enrichment is the important promoting factor of lake eutrophication; And under suitable meteorology, hydrologic condition; Produce biochemical reaction; Discharge thioether class materials such as sulfide, methane and dimethyltrisulfide, form " lake is general ", thereby cause the phenomenon that water quality worsens rapidly, the ecosystem receives heavy damage.Therefore, in the improvement of polluted lake and repairing, the concentration that effectively reduces the content of organic matter and the minimizing in the bed mud and control nitrogen, phosphorus nutrition salt has status of equal importance.0~10cm of the deposit surface that the method for prior art employing dredging removes; Make that annual whole world number is dredged out with hundred million tons deposit; And the deposit that these are dredged out can cause secondary pollution to environment, the more important thing is that large amount of organic is not able to utilize in these deposits of dredging out.If can utilize and be rich in organic deposit and cultivate chlorella, will reach the purpose of killing two birds with one stone.The object of the invention is exactly that cultivation for chlorella provides a kind of new method.
Summary of the invention
To the deficiency and the defective of prior art, technical purpose of the present invention is to propose a kind ofly to be used for chlorella cultured microorganism fuel cell reaction device, and the method for utilizing this microbiological fuel cell reactor to cultivate chlorella.This devices and methods therefor both can reach the purpose of restoration of the ecosystem, again can efficiently cheap cultivation chlorella.
For realizing technical purpose of the present invention, technical scheme of the present invention is following.
One, a kind of chlorella cultured microorganism fuel cell reaction device that is used for mainly is made up of the reative cell, negative electrode, anode, lead, the resistance that contain by water body deposit; Wherein reative cell is the container of other face closures of open-top, and the water body in the reative cell is layered as deposit layer that is positioned at the reative cell bottom and the water body layer that is positioned at reative cell top; Negative electrode is arranged in the water body layer, and anode is arranged in the deposit layer, and through lead negative electrode, anode and resistance is together in series, and resistance is between negative electrode and anode.
Further, described reative cell includes but not limited to cube container, cylindrical container.
Described negative electrode includes but not limited to: graphite, charcoal felt or charcoal cloth.
Described anode includes but not limited to: graphite, charcoal felt or charcoal cloth.
Described resistance is adjustable resistor.
The resistance range value of described adjustable resistor: 10~1000 ohm.
Two, utilize the method that chlorella cultured microorganism fuel cell reaction device is cultivated chlorella that is used for of the present invention, it may further comprise the steps.
The installation setting of A, microbiological fuel cell reactor
Water body deposit is contained in the reative cell, treat after leaving standstill that water body layer and the layering of deposit layer are clear after, anode is placed the deposit layer, negative electrode places the water body layer; Negative electrode, anode and resistance are connected in series with lead, and resistance is between negative electrode and anode.
The operation of B, microbiological fuel cell reactor
Resistance value is set between 10~1000 ohm, promptly begins the operation of reactor naturally, and 25~30 ℃ of value ranges of control reactor working temperature; After anode hung up biomembrane in 5~7 days, measure the content of carbon dioxide in the water body layer.
Wherein, the Determination on content method of the carbon dioxide in the described water body is a gas chromatography.
C, adding chlorella are cultivated
After stable carbon dioxide generating speed is arranged in the water body layer, add chlorella again, with the organic substance in the microbiological fuel cell oxidates to carbon dioxide, thereby be used for the cultivation of chlorella.
Wherein, the addition of described chlorella is: 80~100 mg/L.
The condition of culture of described chlorella is: temperature between 25~30 ℃, incubation time 15~20 days, light dark period 16h:8h, intensity of illumination 2500~3000 ALUX.
Beneficial effect of the present invention is:
(1) the present invention is with the different and advantage of prior art: the present invention utilizes the carbon dioxide that organic substance produces in the microbiological fuel cell oxidates to cultivate chlorella; Compare prior art; Gas concentration lwevel in the water body is high; And obtain easily, and in the process of cultivating, can reach the effect of restoration of the ecosystem simultaneously.
(2) microbe fuel-device of the present invention is simple in structure, does not need too many maintenance, and construction and operating cost are low;
(3) the chlorella cultured microorganism fuel cell reaction device that is used for of the present invention, wherein electrode transmits the electronics that produces in the oxidation of organic compounds process as the electron acceptor of microbe, thereby has quickened organic removal in the substrate.The biology in situ reparation of comparing traditional; It need not add electron acceptor or oxygenate, but with electrode as electron acceptor, can suppress the activity of methanogen; Make the organic substance in the deposit under the effect of anode electrogenesis bacterium, be oxidized to carbon dioxide, and be discharged in the water body.And the chlorella that is arranged in water body can utilize this carbon dioxide, reaches the purpose of cultivating amplification.
Description of drawings
Fig. 1 structural representation of the present invention.
Wherein, 1-anode; The 2-negative electrode; The 3-chlorella; 4-resistance; The 5-lead; The 6-reative cell.
Electric current situation over time in Fig. 2 microbiological fuel cell running.
The biomass of chlorella is measured figure in Fig. 3 reactor.
Embodiment
Through concrete embodiment technical scheme of the present invention is done further explanation below.
Embodiment 1
Present embodiment explanation concrete structure structure and the operation principle that is used for chlorella cultured microorganism fuel cell reaction device of the present invention.
With reference to accompanying drawing 1, the chlorella cultured microorganism fuel cell that is used for of the present invention mainly is made up of the reative cell 6, negative electrode 2, anode 1, lead 5, the resistance 4 that contain by water body deposit; Wherein reative cell 6 is the container of other face closures of open-top, and the water body of 6 li of reative cells is layered as deposit layer that is positioned at the reative cell bottom and the water body layer that is positioned at reative cell top; Negative electrode 2 is arranged in the water body layer, and anode 1 is arranged in the deposit layer, and through lead 5 negative electrode 2, anode 1 and resistance 4 is together in series, and resistance 4 is between negative electrode 2 and anode 1.
Because oxygen in water concentration is from shallow to deep, near anode 1, form anaerobic zone, contain multiple anaerobic bacteria in the deposit layer; Can the organic substance in the deposit be decomposed; Generate hydrogen ion and electronics, electronics flows to negative electrode 2 from anode 1 through lead 5, and hydrogen ion is delivered to negative electrode 2 through water body.Oxygen ability and electronics, hydrogen ion generate water near negative electrode 2, in this process, form electric current, the continuous oxidized removal of the organic substance in the synchronous deposits, and produce a large amount of carbon dioxide, thus be used for the cultivation of upper strata chlorella 3.And be connected with resistance 4 between anode 1 and the negative electrode 2, regulating load resistance is used to optimize the speed of carbon dioxide generating, arrives the effect of regulation and control chlorella growth.
Further, described reative cell includes but not limited to cube container, cylindrical container.
Described negative electrode includes but not limited to: graphite, charcoal felt, charcoal cloth.
Described anode includes but not limited to: graphite, charcoal felt, charcoal cloth.
Described resistance is adjustable resistor.
The resistance range value of described adjustable resistor: 10~1000 ohm.
Embodiment 2
The present embodiment explanation utilizes microbiological fuel cell reactor of the present invention to be used for the cultural method of chlorella.
The installation setting of A, microbiological fuel cell reactor
700 g water body deposits (taking from Nanjing Jin Chuanhe) are contained in the reative cell, treat after leaving standstill that water body layer and the layering of deposit layer are clear after, anode is placed the deposit layer, negative electrode places the water body layer; Negative electrode, anode and resistance are connected in series with lead, and resistance is between negative electrode and anode.
The operation of B, microbiological fuel cell reactor
Resistance value is set between 10~1000 ohm, promptly begins the operation of reactor naturally, and control 25 ℃~30 ℃ of reactor working temperatures; After anode hung up biomembrane in 5~7 days, measure the content of carbon dioxide in the water body layer.
Wherein, the Determination on content method of the carbon dioxide in the described water body is: gas-chromatography is measured.
C, adding chlorella are cultivated
After stable carbon dioxide generating speed is arranged in the water body layer, add chlorella again, with the organic substance in the microbiological fuel cell oxidates to carbon dioxide, thereby be used for the cultivation of chlorella.
Wherein, the addition of described chlorella is: 80~100 mg/L.
The condition of culture of described chlorella is: temperature between 25~30 ℃, incubation time about 15~20 days, light dark period 16h:8h, intensity of illumination 2500~3000 ALUX.
Embodiment 3
Two reactors 1 of present embodiment operation and 2, wherein the negative electrode of reactor 1 uses the charcoal felt, and anode uses the charcoal felt; The negative electrode of reactor 2 uses the charcoal felt, and anode uses the charcoal felt.Wherein reactor 2 outer meeting resistances are 10 ohm, and reactor 1 is that open-circuit condition (not connecting resistance) is as contrast.In 25 ℃ operational environment, move,, determine reactor 2 its outputting current steadilies at 30 mA/m through behind 7 days the anodes extension film (Fig. 2) 2The carbon dioxide of its generation of monitoring is 5 mM in this process, and continual and steady.Afterwards, in reactor 1 and reactor 2, add chlorella, light dark period 16h:8h; Intensity of illumination 3000 ALUX, and continue operation after 15 days measure each reactor Biomass of Chlorella and algae content initial with it (80 mg/L) compares (Fig. 3); The chlorella amount of finding reactor 1 increases few; Have only 110 mg/L, and the chlorella of reactor 2 amount increases significantly, has reached 370 mg/L.Further illustrate the adding of microbiological fuel cell, the organic substance in can the accelerated oxidation deposit becomes carbon dioxide, thereby helps the growth of chlorella.
Embodiment 4
The method of present embodiment is with embodiment 3, and the negative electrode of reactor uses charcoal cloth, anode use graphite.It is following only to change condition.
Reactor 2 outer meeting resistances are 100 ohm.In 30 ℃ operational environment, move, behind 5 days anodes extension film, determine reactor 2 its outputting current steadilies at 18 mA/m 2The carbon dioxide of its generation of monitoring is 4 mM in this process, and continual and steady.Afterwards, add chlorella 100 mg/L at reactor 2, light dark period 16h:8h, intensity of illumination 2500 ALUX, and continue operation after 17 days, chlorella has reached 310 mg/L.
Embodiment 5
The method of present embodiment is with embodiment 3, and the negative electrode of reactor uses graphite, anode use charcoal cloth.It is following only to change condition.
Reactor 2 outer meeting resistances are 1000 ohm.In 26 ℃ operational environment, move, behind 6 days anodes extension film, determine reactor 2 its outputting current steadilies at 6 mA/m 2The carbon dioxide of its generation of monitoring is 2 mM in this process, and continual and steady.Afterwards, in reactor 2, add chlorella 90 mg/L, light dark period 16h:8h, intensity of illumination 2800 ALUX, and continue operation after 20 days, chlorella has reached 200 mg/L.

Claims (10)

1. one kind is used for chlorella cultured microorganism fuel cell reaction device, it is characterized in that mainly being made up of the reative cell, negative electrode, anode, lead, the resistance that contain by water body deposit; Wherein reative cell is the container of other face closures of open-top, and the water body in the reative cell is layered as deposit layer that is positioned at the reative cell bottom and the water body layer that is positioned at reative cell top; Negative electrode is arranged in the water body layer, and anode is arranged in the deposit layer, and through lead negative electrode, anode and resistance is together in series, and resistance is between negative electrode and anode.
2. the chlorella cultured microorganism fuel cell reaction device that is used for according to claim 1 is characterized in that described reative cell is selected from cube container, cylindrical container.
3. the chlorella cultured microorganism fuel cell reaction device that is used for according to claim 1 is characterized in that described negative electrode is: graphite, charcoal felt or charcoal cloth.
4. the chlorella cultured microorganism fuel cell reaction device that is used for according to claim 1 is characterized in that described anode is: graphite, charcoal felt or charcoal cloth.
5. the chlorella cultured microorganism fuel cell reaction device that is used for according to claim 1 is characterized in that described resistance is adjustable resistor.
6. the chlorella cultured microorganism fuel cell reaction device that is used for according to claim 5 is characterized in that the resistance range value of described adjustable resistor: 10~1000 ohm.
7. utilize the described method that is used for chlorella cultured microorganism fuel cell reaction device cultivation chlorella of claim 1, it is characterized in that it may further comprise the steps:
The installation setting of A, microbiological fuel cell reactor
Water body deposit is contained in the reative cell, treat after leaving standstill that water body layer and the layering of deposit layer are clear after, anode is placed the deposit layer, negative electrode places the water body layer; Negative electrode, anode and resistance are connected in series with lead, and resistance is between negative electrode and anode;
The operation of B, microbiological fuel cell reactor
Resistance value is set between 10~1000 ohm, promptly begins the operation of reactor naturally, and 25~30 ℃ of value ranges of control reactor working temperature; After anode hung up biomembrane in 5~7 days, measure the content of carbon dioxide in the water body layer;
C, adding chlorella are cultivated
After stable carbon dioxide generating speed is arranged in the water body layer, add chlorella again, with the organic substance in the microbiological fuel cell oxidates to carbon dioxide, thereby be used for the cultivation of chlorella.
8. method according to claim 7 is characterized in that the Determination on content method of the carbon dioxide in the water body of described step B is a gas chromatography.
9. method according to claim 7 is characterized in that the addition of chlorella among the described step C is: 80~100 mg/L.
10. method according to claim 7 is characterized in that the condition of culture of chlorella among the described step C is: temperature between 25~30 ℃, incubation time 15~20 days, light dark period 16h:8h, intensity of illumination 2500~3000 ALUX.
CN2011103748237A 2011-11-23 2011-11-23 Microbial fuel cell reactor for chlorella culture Expired - Fee Related CN102437361B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105062867A (en) * 2015-09-14 2015-11-18 天津大学 Self-driven ulothrix culturing device and cultural method thereof
CN116768351A (en) * 2023-08-23 2023-09-19 农业农村部成都沼气科学研究所 Electroactive bacteria-algae symbiotic reactor, system and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100941489B1 (en) * 2008-02-27 2010-02-10 건국대학교 산학협력단 Apparatus for culturing algae using using microbial fuel cell and method therefore
WO2010088626A2 (en) * 2009-01-30 2010-08-05 University Of Southern California Electricity generation using phototrophic microbial fuel cells
CN101841053A (en) * 2009-05-12 2010-09-22 中国科学院南京地理与湖泊研究所 Microbiological fuel cell (MFC) and application thereof in removing organisms from natural sediments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100941489B1 (en) * 2008-02-27 2010-02-10 건국대학교 산학협력단 Apparatus for culturing algae using using microbial fuel cell and method therefore
WO2010088626A2 (en) * 2009-01-30 2010-08-05 University Of Southern California Electricity generation using phototrophic microbial fuel cells
CN101841053A (en) * 2009-05-12 2010-09-22 中国科学院南京地理与湖泊研究所 Microbiological fuel cell (MFC) and application thereof in removing organisms from natural sediments

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105062867A (en) * 2015-09-14 2015-11-18 天津大学 Self-driven ulothrix culturing device and cultural method thereof
CN116768351A (en) * 2023-08-23 2023-09-19 农业农村部成都沼气科学研究所 Electroactive bacteria-algae symbiotic reactor, system and application thereof
CN116768351B (en) * 2023-08-23 2023-11-14 农业农村部成都沼气科学研究所 Electroactive bacteria-algae symbiotic reactor, system and application thereof

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