CN102433369A - Comprehensive process and equipment for producing starch through enzymolysis of corn in high proportion - Google Patents
Comprehensive process and equipment for producing starch through enzymolysis of corn in high proportion Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Abstract
The invention belongs to the technical field of enzyme fermentation, and provides a method for deep processing corn through enzymolysis. The method comprises a step of adding acid-resistant cellulase in a high proportion into an aqueous solution of sulphurous acid, so that the production efficiency of deep processing the corn can be improved. In addition, the invention also provides a method for preparing corn starch through enzymolysis, acid-resistant cellulase, a desulfurization treatment system and the like.
Description
Invention field
The invention belongs to the enzymic fermentation technical field, particularly, the present invention relates to the composite technology flow process and the wherein used equipment of enzymolysis Maize Production starch.
Technical background
Corn is one of staple food crop in the world, and China's corn yield is about 100,000,000 tons.The corn most valuable part is its seed.The surface coverage of seed cortex, is plumule and endosperm below cortex, and wherein the plumule oleaginousness is high, and toughness is strong; And the skin of endosperm is wrapped in aleurone layer (slag crust), and the inside is full of starch granules.The maize kernel abundant nutrients except starch, also comprises protein, grease, Mierocrystalline cellulose, soluble sugar, mineral substance etc.
In order effectively to utilize each nutritive ingredient in the corn; What adopt mostly in the present domestic W-Gum processing is comprehensive process flow process as shown in Figure 1, obtains steeping water, maize germ and germ oil, zein fiber, zein and W-Gum respectively through each operation.These operations and equipment thereof are except form of presentation slightly the difference; The introduction of detailed content is all arranged in Chinese patent 02144742 and one Chinese patent application 200510135112; And these two patent documentations have also set forth the recycle of waste water in the technology in detail, but soaking technology and dry back exhaust gas emission and comprehensive utilization aspect are not enlightened to some extent.
In said process, step at first is to soak, and soon normal pressure soaked about 72 hours in about 0.3% (w/w) sulfurous acid aqueous solution of maize kernel about 50 ℃.This step is most important concerning abundant separation steeping water and other products, but owing to used sulfurous acid, causes in the liquids and gases in subsequent step and inevitably can produce the sulfur dioxide pollution thing.For this reason, one Chinese patent application 200810051296 discloses the immersion process of a kind of new W-Gum in producing, and soaking corn in pressurized environment has wherein used the proteolytic enzyme and the cellulase of high density in the soak solution.The characteristics of this method are not use nor can use sulfurous acid to soak; Because wherein the proteolytic enzyme of employed market sale and cellulase just have high reactivity in the neutral and even alkaline environment of pH; The very low and even forfeiture of activity in having added the pH sour environment of sulfurous acid, thereby it has the advantage that reduces sulfur dioxide pollution and soak time weak point.
But; This method also has following shortcoming: immersion needs to use pressurizing vessel; This tests for the testing laboratory that carries out with the 10L scale in this patented claim is feasible, easily in the normal pressure steeping tank of ton, almost can't be transformed into pressurizing vessel in the still present plant produced; Need all to change, cost is high and caused the waste of original steeping tank; Operation and maintenance cost that pressurization needs are higher, and the pressurization back Duo an accident potential than the normal pressure operation, and promptly along with the wearing out of equipment, pressurization can cause the blast of pressurized vessel, and this also is not easy to promote for keeping the safety in production by today of pay attention to day by day; The large usage quantity of use therein proteolytic enzyme, cost is higher, and this is secular running cost; Wherein used proteolytic enzyme, causing protein degradation is that water-soluble polypeptide and amino acid run off, thereby makes follow-up corn protein output significantly reduce, and has greatly reduced the output of the product of a fairly high profit.In addition; Though this method soak time is short, because present domestic W-Gum processing industry is generally from far-off regions, the place is sufficient; The normal pressure steeping tank is cheap and be easy to safeguard in addition, has generally adopted a large amount of steeping tank parallel runnings to overcome the long shortcoming of soak time at present.
The inventor is through long term studies and combine the practice of present domestic W-Gum processing industry, finds that the existing corn deep processing technology that adopts sulfurous acid to soak all only focuses on the sulfurous gas of administering in the liquid, and no matter processing, the proteic preparation of plumule; Still the final finished product of starch that obtains; All need the drying machine drying, drying is evaporated the gas that and is contained more sulfurous gas and be excluded contaminate environment, thereby has invented the new technical scheme of a cover; Under the situation of the equipment such as steeping tank that need not to change present use; At first, the plain enzyme of a high proportion of acid-proof fibre that in immersion process, has used the inventor to find can effectively reduce immersion process Central Asia vitriolic usage quantity; Further reduce the time of required immersion, improved production efficiency; Secondly, keeping soaking under the situation of effect, reducing the dry content that evaporates sulfurous gas in the gas that; Once more; Collect the dry gas that of evaporating,, not only make the heat energy of gas be utilized effectively through the tail gas treatment device of inventor's unitized design; And further remove the sulfurous gas in the gas, make that the sulfur dioxide concentration in the gaseous emission is lower than the 5mg/ cubic meter.
Summary of the invention
The object of the present invention is to provide the method for new enzymolysis corn deep processing, it is under the situation of using the normal pressure soaking device, through adding the plain enzyme of new acid-proof fibre; Reduced the consumption of sulfurous acid; Control the generation of sulfur dioxide gas from the source, and added new dried tail gas treatment facility, not only made full use of the heat energy of tail gas; And eliminate the sulfurous gas in the tail gas basically, make environmentally friendly.In addition, the present invention also provides new plain enzyme of acid-proof fibre and new dried tail gas treatment facility.
Particularly; Aspect first; The invention provides the method for enzymolysis corn deep processing; It is included in the sulfurous acid aqueous solution maize kernel that soaks behind the removal of contamination and soak solution is become steeping water through evaporator evaporation, and corase grind is broken to be soaked the solids that obtains and will roughly grind that the broken plumule that goes out washs successively, dewaters, dry and oil expression is handled, and correct grinding is sieved and washed the broken endosperm that goes out of corase grind and discarded and finish grindes the slag crust of sieving and washing out; Separating correct grinding through separating machine sieves and washes the coarse starch milk that obtains and thereby isolated seitan water is concentrated and dry acquisition protein powder successively; And thereby isolated starch washed successively, dewaters and the dry finished product of starch that obtains, it is characterized in that
(a) as follows or the plain enzyme of acid-proof fibre (b) that add high add-on in the said sulfurous acid aqueous solution,
(a) cellulase of forming by the aminoacid sequence shown in the SEQ ID NO:2;
(b) aminoacid sequence in above-mentioned (a) is through replacing, lack and/or adding one or several amino acid and the acid proof cellulase that obtains;
With, arbitrary, any combination of said drying step or whole tail gas that evaporates are handled through desulfurization.Preferably wherein, thus said with isolated starch wash successively, dewater and the dry process that obtains finished product of starch in drying step evaporate the tail gas that and handle without desulfurization.Because the adding of the plain enzyme of acid-proof fibre; Although what intermediate steps produced still can contain more sulfurous gas, thus sulfurous gas in the tail gas that isolated starch washs successively, dewaters and drying obtains to produce in the process of finished product of starch is considerably less.
In this article, the processing of waste water can be carried out according to the disclosed patent documentation of background technology part.Yet, can be used for the processing that industrial prior art has only been enlightened sulfur dioxide pollution thing in the waste water that corn deep processing is produced, there are not research and practice that the sulfur dioxide pollution thing in the waste gas is handled problems.And the inventor is through studying for a long period of time and putting into practice discovery; In steeping tank, soak,, just can not discharge gas outward as long as steeping tank seals; Even blow-by also can directly feed the tail gas that steeping tank is discharged through pipeline desulfurization in the thionizer of desulfurization treatment system of the present invention; Other are except the tail gas that drying step produces, and miscellaneous part is if not sealing, because temperature is low; Tail gas slow evaporation and quantity is few; Tail gas also can directly feed desulfurization in the thionizer of desulfurization treatment system of the present invention through pipeline, is the soak solution that contains sulfurous acid owing to direct evaporation, and sulfurous gas is dense in the water vapour of evaporator evaporation; It is too many that direct desulfurization does not lose sulfurous acid, therefore after condensation, passes back into steeping tank through pipeline usually; But the tail that produces in the drying step is large-minded, and dry evaporates that sulfurous gas does not reach the effectively content of recycle in the tail gas that.
Therefore, the inventor at first through in immersion process, adding the plain enzyme of new acid-proof fibre, comes effectively to reduce the usage quantity of sulfurous acid, and has reduced the time of soaking; And, under the situation that does not reduce quality product and output, reduced the content of sulfurous gas in the tail gas; In addition, handle the sulfurous gas that further removes in the tail gas, reach eco-friendly effect through desulfurization.Because the content of sulfurous gas reduces from the source in the tail gas, so the running cost of desulfurization in handling reduced, and for example the consumption such as alkali lye such as NaOH solution has just reduced.
In this article; Arbitrary, any combination of drying step or all evaporate the tail gas that and refer to; The broken plumule that goes out of corase grind washs successively, dewaters, in dry and the process that oil expression is handled; Isolated seitan water concentrate successively with the exsiccant process in, and isolated starch washs successively, in dehydration and the exsiccant process, any one, the drying step in two or all processes evaporates the tail gas that.Isolated starch washs successively, in dehydration and the exsiccant process, drying evaporates that content of sulfur dioxide has been lower than some very strict emission control standards in the tail gas that, therefore can handle without desulfurization.Therefore; In the present invention; Arbitrary, any combination of drying step or all evaporate the tail gas that and preferably refer to, except isolated starch washs successively, drying step evaporates all the other two processes the tail gas that in dehydration and the exsiccant process drying step evaporates converging of the tail gas that.
The plain enzymic activity of acid-proof fibre of the present invention is high, and anti-acid environment and produce effects.Can go up output and the quality that reduces zein fiber largely although add the plain enzyme of acid-proof fibre in high proportion, can quicken soak time the most consuming time in the corn deep processing greatly, enhance productivity.Preferably in the method aspect first of the present invention, the add-on of the plain enzyme of said acid-proof fibre is 20～50IU/g maize kernel, is preferably 25～40IU/g maize kernel, more preferably 28～35IU/g maize kernel.
The adding of the plain enzyme of acid-proof fibre of the present invention can effectively reduce the content of sulfurous acid.Preferably in the method aspect first of the present invention, said sulfurous acid aqueous solution Central Asia vitriolic concentration is 0.1～0.28% (w/w), is preferably 0.12～0.2% (w/w), more preferably 0.15～0.18% (w/w).
The equipment that continuity has used at present business enterprise expand at home to use then can significantly reduce improvement cost, also is convenient to popularization of the present invention.Preferably in the method aspect first of the present invention, the pressure of said immersion is normal pressure.Like this, can continue the normal pressure equipment that comprises existing steeping tank that uses.
The adding of the plain enzyme of acid-proof fibre of the present invention can effectively reduce soaks required temperature and time, immersion efficient is provided, and has practiced thrift the energy.Preferably in the method aspect first of the present invention, the temperature of said immersion is 38～60 ℃, is preferably 45～55 ℃, more preferably 48～53 ℃.Also preferably in the method aspect first of the present invention, the time of said immersion is 24～48 hours, is preferably 30～40 hours, more preferably 32～38 hours.
Tail gas can directly feed the thionizer desulfurization, but owing to contain dust in the tail gas, and exhaust temperature is higher, causes upstream device aging easily, and directly getting into thionizer will waste energy, and the easy blocking thionizer.Therefore, in the method aspect first of the present invention, said desulfurization is handled and is comprised with said tail gas vapour pipe and the thionizer through washing tower, blower fan, said vaporizer successively.Washing tower absorbs dust through spray water.Tail gas enough feeds in the vapour pipe of vaporizer through Fan Regulation speed, and soak solution flows through the vapour pipe surface, thereby heating flashes to steeping water with soak solution.Therefore also preferred, said vaporizer will be reduced to 30～60 ℃ from the exhaust temperature of its discharge, be preferably 40～55 ℃, more preferably 45～50 ℃.Save energy so more efficiently.
Aspect second, the invention provides as follows the plain enzyme of (a) or acid-proof fibre (b),
(a) cellulase of forming by the aminoacid sequence shown in the SEQ ID NO:2;
(b) aminoacid sequence in above-mentioned (a) is through replacing, lack and/or adding one or several amino acid and the acid proof cellulase that obtains.This enzyme can be used in the method for the present invention aspect first.
Preferred second aspect of the present invention provides the cellulase of being made up of the aminoacid sequence shown in the SEQ ID NO:2.
Aspect the 3rd, the invention provides the gene of the plain enzyme of the described acid-proof fibre in second aspect of code book invention.According to the DNA recombinant technology, can easily design gene according to known aminoacid sequence.In embodiment of the present invention, the polynucleotide sequence of preferred said gene is shown in SEQ ID NO:1.
Aspect the 4th, the invention provides the carrier that comprises the described gene of third aspect of the present invention, preferably expression vector.Current had many carrier commercializations, can the described gene of third aspect of the present invention be imported in the carrier through conversion, transfection or other recombination means.In embodiment of the present invention, preferred carrier is a pPICZ α A plasmid, and it is a Yeast expression carrier.
Aspect the 5th, the invention provides transform or transfection the host cell of the 4th the described carrier in aspect of the present invention.This host cell can be used for expressing the plain enzyme of the described acid-proof fibre in second aspect of the present invention.The host cell of the 5th aspect of the present invention is yeast preferably, is more preferably pichia spp, and override is pichia spp GS115.
Aspect the 6th; The invention provides the method for preparing the plain enzyme of the described acid-proof fibre in second aspect of the present invention; It is included under the suitable condition of expressing; Cultivate the 5th described cell in aspect of the present invention, and the plain enzyme of the described acid-proof fibre in second aspect of the present invention that gives expression to of purifying.
Aspect the 7th; The invention provides the desulfurization treatment system that is used for corn deep processing; It comprises washing tower, blower fan, vaporizer and thionizer; It is characterized in that said washing tower comprises, can feed arbitrary, any combination of drying step in the corn deep processing or all evaporate the feeding hole of the tail gas that, the spray apertures that can feed water of condensation, sieve plate, water vent and the venting hole that links to each other through pipeline with the inlet mouth of said blower fan; The air outlet of said blower fan links to each other through pipeline with the inlet mouth of the vapour pipe of said vaporizer; Comprise the air inlet port that links to each other through pipeline with the air outlet of the vapour pipe of said vaporizer with, thionizer, can feed limbers, water vent and the venting hole of alkali lye.
Preferably in the desulfurization treatment system of the 7th fermentation of the present invention, feed the hole and be positioned at washing tower lower part, venting hole is positioned at washing tower upper part, helps the water that tail gas sprays through the sieve plate contact like this; Spray apertures is preferably placed at the top of washing tower, helps water like this and more effectively contacts with tail gas from top to bottom; Porose on the sieve plate; Preferred uniform distribution is porose, have in the washing tower in addition one or more (as, two, three, four, five) sieve plate that is arranged in parallel; Preferred sieve plate is arranged on the position that feeds between hole and the venting hole, evenly falls to more effectively contacting with tail gas thereby help water like this; And/or; Water vent is positioned at washing tower lower part than feeding low position, hole, is preferably placed at the bottom of washing tower, like this when the water surface in the washing tower will flood the feeding hole; Can wherein water be discharged, as be discharged in the described Waste Water Treatment of prior art and go.
Preferably in the desulfurization treatment system of the 7th fermentation of the present invention, the vapour pipe of vaporizer is circuitous shape, is more conducive to like this enlarge contact surface and increases vaporization efficiency.
Preferably in the desulfurization treatment system of the 7th fermentation of the present invention, air inlet port be arranged in thionizer alkali lye (like, NaOH solution) liquid level below, be preferably placed at thionizer lower part, thereby make tail gas pass through the alkali lye desulfurization; Venting hole be arranged in thionizer alkali lye (like, NaOH solution) liquid level above, be preferably placed at thionizer upper part, prevent that alkali lye from flowing out from venting hole; And/or water vent is positioned at thionizer lower part, is preferably placed at the bottom of thionizer, about too high or alkali lye pH value is neutralized into 7 at the alkali lye liquid level like this in, expel liquid replenishes alkali lye through the limbers.
The desulfurization treatment system of the 7th aspect of the present invention can be used in the corn deep processing.Therefore, aspect the 8th, the invention provides the application of desulfurization treatment system in the corn deep processing method of the 7th aspect of the present invention, preferably the application in the method for the present invention aspect first.
Beneficial effect of the present invention is: reduce the discharging of sulfurous gas in the gas, and useful to environment; Reduce the consumption of sulfurous acid, reduce cost; Significantly improve the efficient of immersion, save time and the energy; Can technical scale use, except the lower Mierocrystalline cellulose of the output value, do not reduce the output and the quality of other products; Can prolong the equipment that uses with current domestic enterprise, reduce the renewal and renovation of equipment cost, be more convenient for promoting.
For the ease of understanding, below will describe in detail the present invention through concrete accompanying drawing and embodiment.What need particularly point out is that specific examples and accompanying drawing only are in order to explain, not constitute limitation of the scope of the invention.Obviously those of ordinary skill in the art can explain according to this paper, within the scope of the invention the present invention is made various corrections and change, and these corrections and change are also included in the scope of the present invention.In addition, the present invention has quoted open source literature, and these documents also are in order more clearly to describe the present invention, and their full text content is all included the present invention in and carried out reference, just look like they full text in specification sheets of the present invention repeated description the same excessively.
Description of drawings
Fig. 1 has shown the schema of corn deep processing process.
Fig. 2 has shown the schema of desulfurization treating processes of the present invention.
Fig. 3 has shown the synoptic diagram of desulfurization treatment system of the present invention; Wherein, Washing tower 1 comprises, can feed the feeding hole 11 of tail gas A, the spray apertures 12 that can feed water of condensation B, sieve plate 13, water vent 14 and the venting hole 15 that links to each other through pipeline with the inlet mouth 21 of blower fan 2; The inlet mouth 32 of the air outlet 22 of blower fan 2 and the vapour pipe 31 of vaporizer 3 links to each other through pipeline; And; Thionizer 4 comprises the air inlet port 42 that links to each other through pipeline with the air outlet 33 of the vapour pipe 31 of vaporizer 3; Can feed the limbers 42 of alkali lye D, discharge the water vent 43 and the venting hole 44 that is higher than liquid level E of the waste liquid F of environmental sound through neutralizing, it can discharge the waste gas G of environmental sound.
Embodiment
Following this paper will describe invention through concrete embodiment.As do not specialize part, can implement according to listed method in " molecular cloning experiment guide " (third edition) (Cold Spring Harbor laboratory Press) that those skilled in the art were familiar with, " W-Gum manual industry " reference that handbook and this paper quoted such as (China Light Industry Press).In addition, employed material and facility and parts thereof all can be bought from market through commercial sources except that special instruction is arranged among the embodiment.
The clone and the purifying of the plain enzyme of embodiment 1 acid-proof fibre
According to the long-term strain acid resistance penicillium spp (Penicillium purporogenum) that obtains that screens of the inventor; Entrust Institute of Microorganism, Academia Sinica to clone the cellulose enzyme gene of polynucleotide sequence shown in SEQ ID NO:1 through commercial sources; The cellulase of its encoding amino acid sequence shown in SEQ ID NO:2; And be cloned into yeast secreted expression carrier pPICZ α A (can available from Invitrogen company) according to normal frame according to conventional methods and go up and be transformed into pichia spp GS115 strain (can available from Invitrogen company); After PCR is correct with the order-checking check, will expresses the male yeast clone and return the inventor.
According to the explanation of the manufacturer of Invitrogen, yeast clone (single bacterium colony) is inoculated in 25mL BMGH substratum, be cultured to OD600 and reach 5 in 30 ℃, 250rpm jolting.Centrifugal then, abandon supernatant, the yeast body weight is suspended from the 100mL BMMH substratum, cultivate in 30 ℃, 250rpm jolting, during to add final concentration in per 24 hours be the methyl alcohol of 1% (v/v), carried out altogether 3 days.
Then, centrifugal collection supernatant (about 100mL), ice bath add ammonium sulfate and reach 80% saturation ratio; Ice bath was saltoutd 6 hours; Centrifugal 15 minutes of 15000rpm, deposition heavily is dissolved in 20mL 25mM Bis-tris damping fluid (pH5.5), dialysed overnight in 25mM Bis-tris damping fluid (pH5.5).The liquid of dialysing is splined on Q Sepharose Fast Flow chromatography column (available from GE Healthcare); Bis-tris damping fluid (pH5.5) to contain 0～0.3M NaCl carries out gradient elution; Collect wherein maximum peak; Correct and carry out protein quantification through SDS-PAGE detection molecules amount, obtain the plain enzyme liquid of acid-proof fibre thus.
The AZCL-HE-Mierocrystalline cellulose of color mark (can Ltd.) be suspended in the citrate buffer solution of the different pH values of 0.1M (pH4.0～6.5) available from Megazyme International Ireland, be made into the solution that contains AZCL-HE-Mierocrystalline cellulose 0.2% (w/w).The enzyme liquid that in the cellulosic solution of the freshly prepared AZCL-HE-of 200ul, adds the different dilution above-mentioned purifying of 2oul; Hatched 20 minutes in 50 ℃, 500rpm concussion;, centrifugal 5 minutes then, get the absorbancy at thorough 595nm place between supernatant with 3000rpm; With the cellulase standard substance is contrast, calculates enzymic activity.The result is as shown in table 1, shows the acidproof onset of endonuclease capable of the present invention.
Table 1 enzyme of the present invention is to the cellulosic enzymic activity of different pH
| pH | Enzymic activity (IU/mg) |
| 6.5 | 39.8 |
| 5.5 | 127.5 |
| 5.0 | 133.2 |
| 4.5 | 116.7 |
Embodiment 2 enzymolysis corn deep processing processes
As comparative example; As shown in Figure 1; Adopt corn deep processing flow process and the equipment (referring to " W-Gum manual industry ") promoted the use of at home to carry out continuous production, wherein, maize kernel normal pressure in 0.3% (w/w) sulfurous acid aqueous solution of 50 ℃ of having removed impurity soaked 72 hours; Drying step adopts the heat drying step, and the tail gas that the feasible drying machine of carrying out each drying step is discharged remains on 75～95 ℃, and the tail gas of collecting each drying step discharge detects, and the result is following:
The tail gas sulphur dioxide content 550mg/m of drying step in the plumule treating processes
3± 10%;
The tail gas sulphur dioxide content 430mg/m of drying step in the fiber process process
3± 15%;
The tail gas sulphur dioxide content 730mg/m of drying step in the protein powder course of processing
3± 10%; With
The tail gas sulphur dioxide content 40mg/m of drying step in the starch treating processes
3± 15%.
Though the content of sulfur dioxide of above-mentioned tail gas is lower than the source of pollution Air Pollutant Emission limit value of national standard, but still very high, some is near limit value, long-term discharging will make peripheral steel make up and build seriously corroded.
Adopt enzymolysis corn deep processing process of the present invention; Its step and device parameter are all basic identical with above-mentioned comparative example; All differences are following: wherein; The maize kernel of having removed impurity normal pressure in 0.175% (w/w) sulfurous acid aqueous solution of 50 ℃ soaked 36 hours, and the said aqueous solution contains the purified plain enzyme of acid-proof fibre of embodiment 1 of 30IU/g maize kernel; Slag crust influences quality because of cellulose degraded, so do not reclaim; The tail gas of drying step directly enters the atmosphere in the starch treating processes; The tail gas of other drying step converges the back and feeds desulfurization treatment system shown in Figure 3; Thereby through washing tower the time, regulate condensation water quantity exhaust temperature is reduced to 80～85 ℃; Thereby in vaporizer, through amount exhaust temperature is reduced to 45～50 ℃ through regulating blower fan air draft speed and soak solution, when the liquid pH in the thionizer reached 7, expel liquid also fed NaOH solution in addition.Adopt germ oil, zein and finished product of starch that this enzymolysis corn deep processing process produces all not have remarkable quality and output is distinguished, all meet the corresponding product standard with the corresponding product of comparative example production.Collect the tail gas of each drying step discharge and the tail gas detection of handling through the desulfurization treatment system, the result is following:
The tail gas sulphur dioxide content 320mg/m of drying step in the plumule treating processes
3± 15%;
The tail gas sulphur dioxide content 580mg/m of drying step in the protein powder course of processing
3± 15%;
The tail gas G content of sulfur dioxide 0.3mg/m that the desulfurization treatment system is handled
3± 5%; With
The tail gas sulphur dioxide content 15mg/m of drying step in the starch treating processes
3± 15%.
Therefore, adopt the plain enzyme of acid-proof fibre of the present invention, can significantly reduce immersion process Central Asia vitriolic usage quantity, and significantly shorten soak time and reduce soaking temperature; And; Except the lower product of this output value of Mierocrystalline cellulose; Under the situation that does not influence product production and quality, significantly reduce the content of sulfur dioxide in the discharge tail gas, especially iff is produced starch (other intermediate products are handled as liquid debris); Then the tail gas sulphur dioxide content in the starch treating processes even be lower than the Residential areas emission standard of European Union can directly discharge.Further be used in combination desulfurization treatment system of the present invention,, can both make content of sulfur dioxide be reduced to level at the bottom of the utmost point even in each exhaust emissions link of corn deep processing.
Claims (10)
1. the method for enzymolysis corn deep processing; It is included in the sulfurous acid aqueous solution maize kernel that soaks behind the removal of contamination and soak solution is become steeping water through evaporator evaporation; Corase grind is broken to be soaked the solids that obtains and will roughly grind that the broken plumule that goes out washs successively, dewaters, dry and oil expression processing; Correct grinding is sieved and washed the broken endosperm that goes out of corase grind and is discarded the slag crust that correct grinding is sieved and washed out, sieve and wash the coarse starch milk that obtains and isolated seitan water concentrated and the dry protein powder that obtains successively thereby separate correct grinding through separating machine, thereby and isolated starch washed successively, dewaters and dry acquisition finished product of starch; It is characterized in that
(a) as follows or the plain enzyme of acid-proof fibre (b) that add high add-on in the said sulfurous acid aqueous solution,
(a) cellulase of forming by the aminoacid sequence shown in the SEQ ID NO:2;
(b) aminoacid sequence in above-mentioned (a) is through replacing, lack and/or adding one or several amino acid and the acid proof cellulase that obtains;
With, arbitrary, any combination of said drying step or whole tail gas that evaporates are handled through desulfurization.
2. the described method of claim 1 is characterized in that, the cellulase that the plain enzyme of said acid-proof fibre is made up of the aminoacid sequence shown in the SEQ ID NO:2.
3. claim 1 or 2 described methods is characterized in that, the add-on of the plain enzyme of said acid-proof fibre is 20～50IU/g maize kernel, is preferably 25～40IU/g maize kernel, more preferably 28～35IU/g maize kernel; And/or said sulfurous acid aqueous solution Central Asia vitriolic concentration is 0.1～0.28% (w/w), is preferably 0.12～0.2% (w/w), more preferably 0.15～0.18% (w/w).
4. claim 1 or 2 described methods is characterized in that, the pressure of said immersion is normal pressure; The temperature of said immersion is 38～60 ℃, is preferably 45～55 ℃, more preferably 48～53 ℃; And/or the time of said immersion is 24～48 hours, is preferably 30～40 hours, more preferably 32～38 hours.
5. the described method of claim 2 is characterized in that, said desulfurization is handled and comprised vapour pipe and the thionizer that said tail gas is passed through successively washing tower, blower fan, said vaporizer.
6. the described method of claim 5 is characterized in that, said vaporizer will be reduced to 30～60 ℃ by 75～95 ℃ from the exhaust temperature that pipe bundle drier is discharged, and be preferably 40～55 ℃, more preferably 45～50 ℃.
7. the desulfurization treatment system that is used for corn deep processing; It comprises washing tower, blower fan, vaporizer and thionizer; It is characterized in that; Said washing tower comprises, can feed arbitrary, any combination of drying step in the corn deep processing or all evaporate the feeding hole of the tail gas that, the spray apertures that can feed water of condensation, sieve plate, water vent and the venting hole that links to each other through pipeline with the inlet mouth of said blower fan; The air outlet of said blower fan links to each other through pipeline with the inlet mouth of the vapour pipe of said vaporizer; Comprise the air inlet port that links to each other through pipeline with the air outlet of the vapour pipe of said vaporizer with, thionizer, can feed limbers, water vent and the venting hole of alkali lye.
8. the described desulfurization treatment system of claim 7 is characterized in that, feeds the hole and is positioned at washing tower lower part, and venting hole is positioned at washing tower upper part; Spray apertures is positioned at the top of washing tower; Sieve plate is arranged on the position that feeds between hole and the venting hole, and uniform distribution is porose on the sieve plate; And/or water vent is positioned at washing tower lower part and ratio feeds low position, hole.
9. the described desulfurization treatment system of claim 7 is characterized in that, one or more sieve plates that are arranged in parallel are arranged in the washing tower; And/or the vapour pipe of vaporizer is circuitous shape.
10. the application of the arbitrary described treatment system of claim 7～9 in corn deep processing method (the arbitrary described method of preferred claim 2～6).
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1792221A (en) * | 2005-12-23 | 2006-06-28 | 鲁洲生物科技（山东）有限公司 | Tech. of closed water circulation in maize starch prodn. process |
| CN101372702A (en) * | 2008-10-21 | 2009-02-25 | 吉林农业大学 | Method for shortening maize immersion time in cornstarch production process |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1792221A (en) * | 2005-12-23 | 2006-06-28 | 鲁洲生物科技（山东）有限公司 | Tech. of closed water circulation in maize starch prodn. process |
| CN101372702A (en) * | 2008-10-21 | 2009-02-25 | 吉林农业大学 | Method for shortening maize immersion time in cornstarch production process |
Non-Patent Citations (1)
| Title |
|---|
| 王红、汤洁、王筠: "玉米深加工过程中异味气体的来源及其治理措施", 《环境保护》, no. 13, 10 July 2007 (2007-07-10), pages 48 - 50 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022007825A1 (en) * | 2020-07-09 | 2022-01-13 | Novozymes A/S | Improved fiber-washing in corn wet-milling |
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