CN102433368B - Comprehensive process and equipment for producing starch by performing enzymolysis on corn - Google Patents

Comprehensive process and equipment for producing starch by performing enzymolysis on corn Download PDF

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CN102433368B
CN102433368B CN 201110349930 CN201110349930A CN102433368B CN 102433368 B CN102433368 B CN 102433368B CN 201110349930 CN201110349930 CN 201110349930 CN 201110349930 A CN201110349930 A CN 201110349930A CN 102433368 B CN102433368 B CN 102433368B
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sulfurous acid
tail gas
vaporizer
washing tower
acid
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CN102433368A (en
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马吉银
刘鸿
曹洪
刘鑫
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Inner Mongolia Eppen Biotech Co ltd
Ningxia Eppen Biotech Co ltd
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NINGXIA EPPEN BIOTECH CO Ltd
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/14—Drying

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Abstract

The invention belongs to the technical field of enzyme fermentation, and provides a corn enzymolysis deep processing method. The method comprises the following steps of: adding acid-resistant cellulose into a sulfurous acid aqueous solution; and desulfurizing tail gas evaporated in the drying step. Moreover, the invention further provides a method for preparing corn starch by performing enzymolysis based on the method, acid-resistant cellulose, a desulfurizing system and the like.

Description

Composite technology and the equipment thereof of enzymolysis Maize Production starch
Invention field
The invention belongs to the enzymic fermentation technical field, particularly, the present invention relates to the composite technology flow process of enzymolysis Maize Production starch and used equipment wherein.
Technical background
Corn is one of staple food crop in the world, and China's corn yield is about 100,000,000 tons.The corn most valuable part is its seed.The surface coverage of seed cortex, is plumule and endosperm below cortex, plumule oleaginousness height wherein, and toughness is strong; And the skin of endosperm is wrapped in aleurone layer (slag crust), and the inside is full of starch granules.The maize kernel nutritive ingredient is abundant, except starch, also comprises protein, grease, Mierocrystalline cellulose, soluble sugar, mineral substance etc.
In order effectively to utilize each nutritive ingredient in the corn, what adopt mostly in the present domestic W-Gum processing is as shown in Figure 1 comprehensive process flow process, obtains corn steep liquor, maize germ and germ oil, zein fiber, zein and W-Gum respectively by each operation.These operations and equipment thereof are except form of presentation slightly the difference, the introduction of detailed content is arranged in Chinese patent 02144742 and Chinese patent application 200510135112, and these two patent documentations have also elaborated the recycle of waste water in the technology, but soaking technology and dry back exhaust gas emission and comprehensive utilization aspect are not enlightened to some extent.
In said process, step at first is to soak, and soon normal pressure soaked about 72 hours in about 0.3% (w/w) sulfurous acid aqueous solution of maize kernel about 50 ℃.This step is most important concerning abundant separation corn steep liquor and other products, but owing to used sulfurous acid, causes in the liquids and gases in subsequent step and inevitably can produce the sulfur dioxide pollution thing.For this reason, Chinese patent application 200810051296 discloses the immersion process of a kind of new W-Gum in producing, and soaking corn in pressurized environment has wherein used proteolytic enzyme and the cellulase of high density in the soak solution.The characteristics of this method are not use nor can use sulfurous acid to soak, because wherein the proteolytic enzyme of employed market sale and cellulase just have high reactivity in the neutral and even alkaline environment of pH, the very low and even forfeiture of activity in having added the pH sour environment of sulfurous acid, thereby it has the advantage that reduces sulfur dioxide pollution and soak time weak point.
But, this method also has following shortcoming: soaking needs to use pressurizing vessel, this tests for the testing laboratory that carries out with the 10L scale in this patent application is feasible, but now in the plant produced easily in the normal pressure steeping tank of ton, almost can't be transformed into pressurizing vessel, need all to change, cost is high and caused the waste of original steeping tank; Operation and maintenance cost that pressurization needs are higher, and the pressurization back Duo an accident potential than the normal pressure operation, i.e. aging along with equipment, and pressurization can cause the blast of pressurized vessel, this also is not easy to promote for keeping the safety in production by today of pay attention to day by day; The large usage quantity of use therein proteolytic enzyme and cellulase, cost is higher, and this is long-term running cost; Wherein used proteolytic enzyme, causing protein degradation is that water-soluble polypeptide and amino acid run off, thereby makes follow-up corn protein output significantly reduce, and has greatly reduced the output of the product of a fairly high profit.In addition, though this method soak time is short, because present domestic W-Gum processing industry is generally from far-off regions, the place abundance, the normal pressure steeping tank is cheap and be easy to safeguard in addition, has generally adopted a large amount of steeping tank parallel runnings to overcome the long shortcoming of soak time at present.
The inventor is through long term studies and in conjunction with the practice of present domestic W-Gum processing industry, find that the existing corn deep processing technology that adopts sulfurous acid to soak all only focuses on the sulfurous gas of administering in the liquid, and the no matter processing of plumule, the acquisition of fiber, the preparation of albumen, still finally obtain finished product of starch, all need the drying machine drying, the gas that drying evaporates contains more sulfurous gas and is excluded contaminate environment, thereby invented one and overlapped new technical scheme, under the situation of the equipment such as steeping tank that need not to change present use, at first, the plain enzyme of the acid-proof fibre that in immersion process, has used the inventor to find, can effectively reduce the usage quantity of sulfurous acid in the immersion process, keeping soaking under the situation of effect, reducing the content of sulfurous gas in the dry gas that evaporates; Secondly, collect the dry gas that evaporates, by the tail gas treatment device of inventor's unitized design, not only make the heat energy of gas be utilized effectively, and further remove sulfurous gas in the gas, make that the sulfur dioxide concentration in the gaseous emission is lower than the 5mg/ cubic meter.
Summary of the invention
The object of the present invention is to provide the method for new enzymolysis corn deep processing, it is under the situation of using the normal pressure soaking device, by adding the plain enzyme of new acid-proof fibre, reduced the consumption of sulfurous acid, control the generation of sulfur dioxide gas from the source, and added new dried tail gas treatment facility, not only taken full advantage of the heat energy of tail gas, and eliminate sulfurous gas in the tail gas basically, make environmentally friendly.In addition, the present invention also provides the new plain enzyme of acid-proof fibre and new dried tail gas treatment facility.
Particularly, aspect first, the invention provides the method for enzymolysis corn deep processing, it is included in the sulfurous acid aqueous solution maize kernel that soaks behind the removal of contamination and soak solution is become corn steep liquor through evaporator evaporation, corase grind is broken to be soaked the solids that obtains and will roughly grind the plumule that fragmentation goes out and wash successively, dehydration, dry and oil expression is handled, correct grinding is sieved and washed the broken endosperm that goes out of corase grind and thereby the slag crust that correct grinding is sieved and washed out is dewatered and the dry fiber that obtains successively, separating correct grinding through separating machine sieves and washes the coarse starch milk that obtains and thereby isolated seitan water is concentrated and dry acquisition protein powder successively, and isolated starch washed successively, thereby dehydration and the dry finished product of starch that obtains, it is characterized in that
Add the plain enzyme of following (a) or acid-proof fibre (b) in the described sulfurous acid aqueous solution,
(a) cellulase of being formed by the aminoacid sequence shown in the SEQ ID NO:2;
(b) aminoacid sequence in above-mentioned (a) is through replacing, lack and/or adding one or several amino acid and the acid proof cellulase that obtains;
With, arbitrary, any combination of described drying step or whole tail gas that evaporates are handled through desulfurization.Preferably wherein, thus described isolated starch is washed successively, dewaters and the dry process that obtains finished product of starch in the tail gas that evaporates of drying step handle without desulfurization.Because the adding of the plain enzyme of acid-proof fibre, although what intermediate steps produced still can contain more sulfurous gas, thus isolated starch washed successively, dewaters and the process of dry acquisition finished product of starch in the tail gas that produces sulfurous gas considerably less.
In this article, the processing of waste water can be carried out according to the disclosed patent documentation of background technology part.Yet, can be used for the processing that industrial prior art has only been enlightened sulfur dioxide pollution thing in the waste water that corn deep processing is produced, there are not research and practice that the sulfur dioxide pollution thing in the waste gas is handled problems.And the inventor is through studying for a long period of time and putting into practice discovery, in steeping tank, soak, as long as steeping tank seals, just can not discharge gas outward, even blow-by, also the tail gas that steeping tank can be discharged directly feed desulfurization in the thionizer of desulfurization treatment system of the present invention by pipeline; Other are except the tail gas that drying step produces, miscellaneous part if not the sealing, because temperature is low, tail gas slow evaporation and quantity is few, tail gas also can directly feed desulfurization in the thionizer of desulfurization treatment system of the present invention by pipeline, is the soak solution that contains sulfurous acid owing to direct evaporation, and sulfurous gas is dense in the water vapour of evaporator evaporation, it is too many that direct desulfurization does not lose sulfurous acid, therefore passes back into steeping tank by pipeline usually after condensation; But the tail that produces in the drying step is large-minded, and sulfurous gas does not reach the effectively content of recycle in the dry tail gas that evaporates.
Therefore, the inventor at first by add the plain enzyme of new acid-proof fibre in immersion process, effectively reduces the usage quantity of sulfurous acid, under the situation that does not reduce quality product and output, has reduced the content of sulfurous gas in the tail gas; Secondly, handle the sulfurous gas that further removes in the tail gas by desulfurization, reach eco-friendly effect.Because the content of sulfurous gas reduces from the source in the tail gas, so the running cost of desulfurization in handling reduced, and for example the consumption such as alkali lye such as NaOH solution has just reduced.
In this article, arbitrary, any combination or whole tail gas that evaporates of drying step refer to, the broken plumule that goes out of corase grind washs successively, dewaters, in dry and the process that oil expression is handled, the slag crust sieved and washed out of correct grinding dewater successively and dry process in, isolated seitan water concentrate successively and dry process in, and isolated starch washs successively, dewaters and dry process in, any one, the tail gas that evaporates of the drying step in two, three or all processes.Isolated starch wash successively, dewater and dry process in, content of sulfur dioxide has been lower than some very strict emission control standards in the tail gas that drying evaporates, and therefore can handle without desulfurization.Therefore, in the present invention, arbitrary, any combination or whole tail gas that evaporates of drying step preferably refer to, except isolated starch wash successively, dewater and dry process in the converging of the tail gas that evaporates of the drying step of its excess-three process the tail gas that evaporates of drying step.
Therefore, preferably of the present invention aspect first, iff other intermediate products no matter in order to prepare cornstarch, the invention provides the method that enzymolysis prepares cornstarch, it is included in the maize kernel after the immersion removal of contamination in the sulfurous acid aqueous solution, the broken solids that obtains that soaks of corase grind, fine grinding is sieved and washed the endosperm that the corase grind fragmentation goes out, separate fine grinding through seperator and sieve and wash the coarse starch milk that obtains, thereby and isolated starch is washed successively, dewaters and the dry finished product of starch that obtains, it is characterized in that
Add the plain enzyme of following (a) or acid-proof fibre (b) in the described sulfurous acid aqueous solution,
(a) cellulase of being formed by the aminoacid sequence shown in the SEQ ID NO:2;
(b) aminoacid sequence in above-mentioned (a) is through replacing, lack and/or adding one or several amino acid and the acid proof cellulase that obtains;
And choose wantonly more preferably, the tail gas that described drying step evaporates is handled without desulfurization.Because the adding of the plain enzyme of acid-proof fibre, although what intermediate steps produced still can contain more sulfurous gas, thus isolated starch washed successively, dewaters and the process of dry acquisition finished product of starch in the tail gas that produces sulfurous gas considerably less.
The plain enzymic activity height of acid-proof fibre of the present invention, and anti-acid environment and produce effects reduce the output of zein fiber for fear of its effectively hydrolyzing, uses this less acid-proof fibre element enzyme, have so also saved running cost.Preferably in the method aspect first of the present invention, the add-on of the plain enzyme of described acid-proof fibre is 1～12IU/g maize kernel, is preferably 2～8IU/g maize kernel, more preferably 3～5IU/g maize kernel.
The adding of the plain enzyme of acid-proof fibre of the present invention can effectively reduce the content of sulfurous acid.Preferably in the method aspect first of the present invention, the concentration of sulfurous acid is 0.1～0.28% (w/w) in the described sulfurous acid aqueous solution, is preferably 0.12～0.2% (w/w), more preferably 0.15～0.18% (w/w).
The equipment that continuity has used at present business enterprise expand at home to use then can significantly reduce improvement cost, also is convenient to popularization of the present invention.Preferably in the method aspect first of the present invention, the pressure of described immersion is normal pressure.Like this, can continue the normal pressure equipment that comprises existing steeping tank that uses.
The adding of the plain enzyme of acid-proof fibre of the present invention can effectively reduce soaks required temperature and time, immersion efficient is provided, and has saved the energy.Preferably in the method aspect first of the present invention, the temperature of described immersion is 38～60 ℃, is preferably 45～55 ℃, more preferably 48～53 ℃.Also preferably in the method aspect first of the present invention, the time of described immersion is 35～70 hours, is preferably 40～60 hours, more preferably 45～54 hours.
Tail gas can directly feed the thionizer desulfurization, but owing to contain dust in the tail gas, and exhaust temperature is higher, causes upstream device aging easily, and directly entering thionizer will waste energy, and stop up thionizer easily.Therefore, in the method aspect first of the present invention, described desulfurization is handled and is comprised with described tail gas vapour pipe and the thionizer by washing tower, blower fan, described vaporizer successively.Washing tower absorbs dust by spray water.Tail gas enough feeds in the vapour pipe of vaporizer through Fan Regulation speed, and soak solution flows through the vapour pipe surface, thereby heating flashes to corn steep liquor with soak solution.Therefore also preferred, described vaporizer will be reduced to 30～60 ℃ from the exhaust temperature of its discharge, be preferably 40～55 ℃, more preferably 45～50 ℃.Save energy so more efficiently.
Aspect second, the invention provides the plain enzyme of following (a) or acid-proof fibre (b),
(a) cellulase of being formed by the aminoacid sequence shown in the SEQ ID NO:2;
(b) aminoacid sequence in above-mentioned (a) is through replacing, lack and/or adding one or several amino acid and the acid proof cellulase that obtains.This enzyme can be used in the method for the present invention aspect first.
Preferred second aspect of the present invention provides the cellulase of being made up of the aminoacid sequence shown in the SEQ ID NO:2.
Aspect the 3rd, the invention provides the gene of the plain enzyme of the described acid-proof fibre in second aspect of code book invention.According to the DNA recombinant technology, can easily design gene according to known aminoacid sequence.In the specific embodiment of the present invention, the polynucleotide sequence of preferred described gene is shown in SEQ ID NO:1.
Aspect the 4th, the invention provides the carrier that comprises the described gene of third aspect of the present invention, preferably expression vector.Current had many carrier commercializations, the described gene of third aspect of the present invention can be imported in the carrier by conversion, transfection or other gene recombination means.In the specific embodiment of the present invention, preferred carrier is pPICZ α A plasmid, and it is Yeast expression carrier.
Aspect the 5th, the invention provides transform or transfection the host cell of the 4th the described carrier in aspect of the present invention.This host cell can be used for expressing the plain enzyme of the described acid-proof fibre in second aspect of the present invention.The host cell of the 5th aspect of the present invention is yeast preferably, is more preferably pichia spp, and override is pichia spp GS115.
Aspect the 6th, the invention provides the method for preparing the plain enzyme of the described acid-proof fibre in second aspect of the present invention, it is included under the suitable condition of expressing, cultivate the 5th described cell in aspect of the present invention, and the plain enzyme of the described acid-proof fibre in second aspect of the present invention that gives expression to of purifying.
Aspect the 7th, the invention provides the desulfurization treatment system for corn deep processing, it comprises washing tower, blower fan, vaporizer and thionizer, it is characterized in that, described washing tower comprises, can feed arbitrary, any combination of drying step in the corn deep processing or the feeding hole of whole tail gas that evaporate, the spray apertures that can feed water of condensation, sieve plate, water vent and the venting hole that links to each other by pipeline with the inlet mouth of described blower fan; The air outlet of described blower fan links to each other by pipeline with the inlet mouth of the vapour pipe of described vaporizer; Comprise the air inlet port that links to each other by pipeline with the air outlet of the vapour pipe of described vaporizer with, thionizer, can feed limbers, water vent and the transfer port of alkali lye.
Preferably in the desulfurization treatment system of the 7th fermentation of the present invention, feed the hole and be positioned at washing tower lower part, venting hole is positioned at washing tower upper part, is conducive to the water that tail gas sprays by the sieve plate contact like this; Spray apertures is preferably placed at the top of washing tower, is conducive to water like this and more effectively contacts with tail gas from top to bottom; Porose on the sieve plate, preferably be evenly distributed with the hole, have in the washing tower in addition one or more (as, two, three, four, five) sieve plate that is arranged in parallel, preferred sieve plate is arranged on the position that feeds between hole and the venting hole, evenly falls to more effectively contacting with tail gas thereby be conducive to water like this; And/or, water vent is positioned at washing tower lower part than feeding low position, hole, is preferably placed at the bottom of washing tower, like this when the water surface in the washing tower will flood the feeding hole, wherein water can be discharged, as be discharged in the described Waste Water Treatment of prior art and go.
Preferably in the desulfurization treatment system of the 7th fermentation of the present invention, the vapour pipe of vaporizer is circuitous shape, is more conducive to like this enlarge contact surface and increases vaporization efficiency.
Preferably in the desulfurization treatment system of the 7th fermentation of the present invention, air inlet port be arranged in thionizer alkali lye (as, NaOH solution) liquid level below, be preferably placed at thionizer lower part, thereby make tail gas pass through the alkali lye desulfurization; Venting hole be arranged in thionizer alkali lye (as, NaOH solution) liquid level above, be preferably placed at thionizer upper part, prevent that alkali lye from flowing out from venting hole; And/or water vent is positioned at thionizer lower part, is preferably placed at the bottom of thionizer, about too high or alkali lye pH value is neutralized into 7 at the alkali lye liquid level like this in, expel liquid replenishes alkali lye by the limbers.
The desulfurization treatment system of the 7th aspect of the present invention can be used in the corn deep processing.Therefore, aspect the 8th, the invention provides the application of desulfurization treatment system in the corn deep processing method of the 7th aspect of the present invention, preferably the application in the method for the present invention aspect first.
Beneficial effect of the present invention is: reduce the discharging of sulfurous gas in the gas, and useful to environment; Reduce the consumption of sulfurous acid, reduce cost; Reduced the time of soaking to a certain extent, improved the efficient of soaking, saved time and the energy; Can technical scale use, do not reduce output and the quality of product; Can prolong the equipment that uses with current domestic enterprise, reduce the renewal and renovation of equipment cost, be more convenient for promoting.
For the ease of understanding, below will the present invention be described in detail by concrete drawings and Examples.It needs to be noted that specific examples and accompanying drawing only are in order to illustrate, not constitute limitation of the scope of the invention.Obviously those of ordinary skill in the art can illustrate according to this paper, within the scope of the invention the present invention is made various corrections and change, and these corrections and change are also included in the scope of the present invention.In addition, the present invention has quoted open source literature, and these documents also are in order more clearly to describe the present invention, and their full text content is all included the present invention in and carried out reference, just look like they full text in specification sheets of the present invention repeated description the same excessively.
Description of drawings
Fig. 1 has shown the schema of corn deep processing process.
Fig. 2 has shown the schema of desulfurization treating processes of the present invention.
Fig. 3 has shown the synoptic diagram of desulfurization treatment system of the present invention, wherein, washing tower 1 comprises, can feed the feeding hole 11 of tail gas A, the spray apertures 12 that can feed water of condensation B, sieve plate 13, water vent 14 and the venting hole 15 that links to each other by pipeline with the inlet mouth 21 of blower fan 2; The inlet mouth 32 of the air outlet 22 of blower fan 2 and the vapour pipe 31 of vaporizer 3 links to each other by pipeline; And, thionizer 4 comprises the air inlet port 42 that links to each other by pipeline with the air outlet 33 of the vapour pipe 31 of vaporizer 3, can feed the limbers 42 of alkali lye D, discharge through neutralization and the water vent 43 and the venting hole 44 that is higher than liquid level E of the waste liquid F of environmental sound, it can discharge the waste gas G of environmental sound.
Embodiment
Following this paper will describe invention by specific embodiment.As not specializing part, listed method is implemented in the reference that can quote according to " molecular cloning experiment guide " (third edition) (Cold Spring Harbor laboratory Press), " W-Gum manual industry " handbooks such as (China Light Industry Press) and this paper that those skilled in the art were familiar with.In addition, employed material and facility and parts thereof all can be bought by commercial sources except special instruction is arranged from the market among the embodiment.
Clone and the purifying of the plain enzyme of embodiment 1 acid-proof fibre
According to the long-term strain acid resistance Penicillium notatum (Penicillium purporogenum) that obtains that screens of the inventor, entrust Institute of Microorganism, Academia Sinica to clone the cellulose enzyme gene of polynucleotide sequence shown in SEQ ID NO:1 by commercial sources, the cellulase of its encoding amino acid sequence shown in SEQ ID NO:2, and be cloned into yeast secreted expression carrier pPICZ α A (can available from Invitrogen company) according to normal frame according to conventional methods and go up and be transformed into pichia spp GS115 strain (can available from Invitrogen company), after PCR and order-checking check correctly, return the inventor with expressing positive yeast clone.
According to the explanation of the manufacturer of Invitrogen, yeast clone (single bacterium colony) is inoculated in 25mL BMGH substratum, be cultured to OD600 and reach 5 in 30 ℃, 250rpm jolting.Centrifugal then, abandon supernatant, the yeast body weight is suspended from the 100mL BMMH substratum, cultivate in 30 ℃, 250rpm jolting, during to add final concentration in per 24 hours be the methyl alcohol of 1% (v/v), carried out altogether 3 days.
Then, centrifugal collection supernatant liquor (about 100mL), ice bath add ammonium sulfate and reach 80% saturation ratio, ice bath was saltoutd 6 hours, centrifugal 15 minutes of 15000rpm, precipitation heavily is dissolved in 20mL 25mM Bis-tris damping fluid (pH5.5), dialysed overnight in 25mM Bis-tris damping fluid (pH5.5).The liquid of dialysing is splined on Q Sepharose Fast Flow chromatography column (available from GE Healthcare), carry out gradient elution with the Bis-tris damping fluid (pH5.5) that contains 0～0.3M NaCl, collect wherein maximum peak, correct and carry out protein quantification through SDS-PAGE detection molecules amount, obtain the plain enzyme liquid of acid-proof fibre thus.
The AZCL-HE-Mierocrystalline cellulose of color mark (can Ltd.) be suspended in the citrate buffer solution of the different pH values of 0.1M (pH4.0～6.5) available from Megazyme International Ireland, be made into the solution that contains AZCL-HE-Mierocrystalline cellulose 0.2% (w/w).The enzyme liquid that adds the different dilution above-mentioned purifying of 2oul in the cellulosic solution of the freshly prepared AZCL-HE-of 200ul, hatched 20 minutes in 50 ℃, 500rpm concussion, with 3000rpm centrifugal 5 minutes then, get the absorbancy at thorough 595nm place between supernatant liquor, be contrast with the cellulase standard substance, calculate enzymic activity.The result is as shown in table 1, shows the acidproof onset of endonuclease capable of the present invention.
Table 1 enzyme of the present invention is to the cellulosic enzymic activity of different pH
pH Enzymic activity (IU/mg)
6.5 39.8
5.5 127.5
5.0 133.2
4.5 116.7
Embodiment 2 enzymolysis corn deep processing processes
As a comparative example, as shown in Figure 1, adopt corn deep processing flow process and the equipment (referring to " W-Gum manual industry ") promoted the use of at home to produce continuously, wherein, maize kernel normal pressure in 0.3% (w/w) sulfurous acid aqueous solution of 50 ℃ of having removed impurity soaked 72 hours; Drying step adopts the heat drying step, and the tail gas that the feasible drying machine of carrying out each drying step is discharged remains on 75～95 ℃, and the tail gas of collecting each drying step discharge detects, and the result is as follows:
The tail gas sulphur dioxide content 550mg/m of drying step in the plumule treating processes 3± 10%;
The tail gas sulphur dioxide content 430mg/m of drying step in the fiber process process 3± 15%;
The tail gas sulphur dioxide content 730mg/m of drying step in the protein powder course of processing 3± 10%; With
The tail gas sulphur dioxide content 40mg/m of drying step in the starch treating processes 3± 15%.
Though the content of sulfur dioxide of above-mentioned tail gas is lower than the source of pollution Air Pollutant Emission limit value of national standard, but still very high, some is near limit value, long-term discharging will make peripheral steel make up and build seriously corroded.
Adopt enzymolysis corn deep processing process of the present invention, its step and device parameter are all basic identical with above-mentioned comparative example, all differences are as follows: wherein, the maize kernel of having removed impurity normal pressure in 0.175% (w/w) sulfurous acid aqueous solution of 50 ℃ soaked 50 hours, and the described aqueous solution contains the purified plain enzyme of acid-proof fibre of embodiment 1 of 3.5IU/g maize kernel; The tail gas of drying step directly enters the atmosphere in the starch treating processes, the tail gas of other drying step converges the back and feeds desulfurization treatment system shown in Figure 3, thereby by washing tower the time, regulate condensation water quantity exhaust temperature is reduced to 80～85 ℃, thereby in vaporizer, by regulating blower fan air draft speed and soak solution throughput exhaust temperature is reduced to 45～50 ℃, when the liquid pH in the thionizer reached 7, expel liquid also fed NaOH solution in addition.Adopt germ oil, zein fiber, zein and finished product of starch that this enzymolysis corn deep processing process produces all not have remarkable quality with the corresponding product of comparative example production and output is distinguished, all meet the corresponding product standard.Collect the tail gas of each drying step discharge and the tail gas detection of handling through the desulfurization treatment system, the result is as follows:
The tail gas sulphur dioxide content 270mg/m of drying step in the plumule treating processes 3± 15%;
The tail gas sulphur dioxide content 210mg/m of drying step in the fiber process process 3± 15%;
The tail gas sulphur dioxide content 560mg/m of drying step in the protein powder course of processing 3± 10%;
The tail gas G content of sulfur dioxide 0.3mg/m that the desulfurization treatment system is handled 3± 5%; With
The tail gas sulphur dioxide content 15mg/m of drying step in the starch treating processes 3± 15%.
Therefore, adopt the plain enzyme of acid-proof fibre of the present invention, can significantly reduce the usage quantity of sulfurous acid in the immersion process, shorten soak time and reduce soaking temperature; And, under the situation that does not influence product production and quality, significantly reduce the content of sulfur dioxide in the discharge tail gas, especially iff producing starch (other intermediate products are handled as liquid debris), then the tail gas sulphur dioxide content in the starch treating processes even be lower than the Residential areas emission standard of European Union can directly discharge.Further combined with using desulfurization treatment system of the present invention, even in each exhaust emissions link of corn deep processing, can both make content of sulfur dioxide be reduced to level at the bottom of the utmost point.
Figure ISA00000607909600011
Figure ISA00000607909600021
Figure ISA00000607909600031
Figure ISA00000607909600041

Claims (23)

1. enzymolysis prepares the method for W-Gum, it is included in the maize kernel behind the immersion removal of contamination in the sulfurous acid aqueous solution, the broken solids that obtains that soaks of corase grind, correct grinding is sieved and washed the endosperm that the corase grind fragmentation goes out, separate correct grinding through separating machine and sieve and wash the coarse starch milk that obtains, thereby and isolated starch washed successively, dewaters and the dry finished product of starch that obtains, it is characterized in that
The plain enzyme of acid-proof fibre that adds following (a) in the described sulfurous acid aqueous solution,
(a) cellulase of being formed by the aminoacid sequence shown in the SEQ ID NO:2.
2. the method for enzymolysis corn deep processing, it is included in the sulfurous acid aqueous solution maize kernel that soaks behind the removal of contamination and soak solution is become corn steep liquor through evaporator evaporation, corase grind is broken to be soaked the solids that obtains and will roughly grind the plumule that fragmentation goes out and wash successively, dehydration, dry and oil expression is handled, correct grinding is sieved and washed the broken endosperm that goes out of corase grind and thereby the slag crust that correct grinding is sieved and washed out is dewatered and the dry fiber that obtains successively, separating correct grinding through separating machine sieves and washes the coarse starch milk that obtains and thereby isolated seitan water is concentrated and dry acquisition protein powder successively, and isolated starch washed successively, thereby dehydration and the dry finished product of starch that obtains, it is characterized in that
The plain enzyme of acid-proof fibre that adds following (a) in the described sulfurous acid aqueous solution,
(a) cellulase of being formed by the aminoacid sequence shown in the SEQ ID NO:2;
With, arbitrary, any combination of described drying step or whole tail gas that evaporates are handled through desulfurization.
3. claim 1 or 2 described methods is characterized in that, the add-on of the plain enzyme of described acid-proof fibre is 1～12IU/g maize kernel.
4. the described method of claim 3 is characterized in that, the add-on of the plain enzyme of described acid-proof fibre is 2～8IU/g maize kernel.
5. the described method of claim 4 is characterized in that, the add-on of the plain enzyme of described acid-proof fibre is 3～5IU/g maize kernel.
6. claim 1 or 2 described methods is characterized in that, the concentration of sulfurous acid is 0.1～0.28% (w/w) in the described sulfurous acid aqueous solution.
7. the described method of claim 6 is characterized in that, the concentration of sulfurous acid is 0.12～0.2% (w/w) in the described sulfurous acid aqueous solution.
8. the described method of claim 7 is characterized in that, the concentration of sulfurous acid is 0.15～0.18% (w/w) in the described sulfurous acid aqueous solution.
9. claim 1 or 2 described methods is characterized in that, the pressure of described immersion is normal pressure.
10. claim 1 or 2 described methods is characterized in that, the temperature of described immersion is 38～60 ℃.
11. the described method of claim 10 is characterized in that, the temperature of described immersion is 45～55 ℃.
12. the described method of claim 11 is characterized in that, the temperature of described immersion is 48～53 ℃.
13. claim 1 or 2 described methods is characterized in that the time of described immersion is 35～70 hours.
14. the described method of claim 13 is characterized in that, the time of described immersion is 40～60 hours.
15. the described method of claim 14 is characterized in that, the time of described immersion is 45～54 hours.
16. the described method of claim 2 is characterized in that, described desulfurization is handled and is comprised vapour pipe and the thionizer that described tail gas is passed through successively washing tower, blower fan, described vaporizer.
17. the described method of claim 16 is characterized in that, described vaporizer will be reduced to 30～60 ℃ by 75～95 ℃ from the exhaust temperature that pipe bundle drier is discharged.
18. the described method of claim 17 is characterized in that, described vaporizer will be reduced to 40～55 ℃ by 75～95 ℃ from the exhaust temperature that pipe bundle drier is discharged.
19. the described method of claim 18 is characterized in that, described vaporizer will be reduced to 45～50 ℃ by 75～95 ℃ from the exhaust temperature that pipe bundle drier is discharged.
The plain enzyme of the acid-proof fibre of following 20. (a),
(a) cellulase of being formed by the aminoacid sequence shown in the SEQ ID NO:2.
21. be used for the desulfurization treatment system of corn deep processing, it comprises washing tower, blower fan, vaporizer and thionizer, it is characterized in that, described washing tower comprises, can feed arbitrary, any combination of drying step in the method for the described enzymolysis corn deep processing of claim 2 or the feeding hole of whole tail gas that evaporate, the spray apertures that can feed water of condensation, sieve plate, water vent and the venting hole that links to each other by pipeline with the inlet mouth of described blower fan; The air outlet of described blower fan links to each other by pipeline with the inlet mouth of the vapour pipe of described vaporizer; Comprise the air inlet port that links to each other by pipeline with the air outlet of the vapour pipe of described vaporizer with, thionizer, can feed the limbers of alkali lye, water vent and venting hole,
And wherein, feed the hole and be positioned at washing tower lower part, venting hole is positioned at washing tower upper part; Spray apertures is positioned at the top of washing tower; One or more sieve plates that are arranged in parallel are arranged in the washing tower, be evenly distributed with the hole on the sieve plate, sieve plate is arranged on the position that feeds between hole and the venting hole; Water vent is positioned at washing tower lower part than feeding low position, hole; The vapour pipe of vaporizer is circuitous shape; Air inlet port is positioned at thionizer lower part; Venting hole is positioned at thionizer upper part; And water vent is positioned at thionizer lower part.
22. the application of the described treatment system of claim 21 in the corn deep processing method.
23. the application of the described treatment system of claim 21 in claim 2,16 ~ 19 arbitrary described method.
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