CN102433277A - Chryseobacterium sp. for removing phosphorus in sewage at low temperature and separation culture method - Google Patents
Chryseobacterium sp. for removing phosphorus in sewage at low temperature and separation culture method Download PDFInfo
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- CN102433277A CN102433277A CN2011104162872A CN201110416287A CN102433277A CN 102433277 A CN102433277 A CN 102433277A CN 2011104162872 A CN2011104162872 A CN 2011104162872A CN 201110416287 A CN201110416287 A CN 201110416287A CN 102433277 A CN102433277 A CN 102433277A
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses Chryseobacterium sp. for removing phosphorus in sewage at low temperature and a separation culture method. The Chryseobacterium sp. deep yellow polyphosphorus CGMCC No.5282 for removing the phosphorus in the sewage at low temperature has the ability of removing the phosphorus in the sewage at low temperature under aerobic conditions. The experimental result shows that: in spite of obvious inhibition of a low-temperature environment, the Chryseobacterium sp. deep yellow polyphosphorus CGMCC No.5282 for removing the phosphorus in the sewage at low temperature has a remarkable effect of strengthening the removal of the phosphorus at low temperature, especially at the temperature of between 8 and 12 DEG C, and the removal rate of the phosphorus is improved by 10 percent; and the effluent standard is superior to the first-grade A effluent standard (water temperature is less than or equal to 12 DEG C, and the control index of the first-grade A standard is 0.5mg/L).
Description
Technical field
The invention belongs to sewage treatment area, relate to a strain and have low temperature and go down except that the golden yellow bacillus and the isolation cultivation method of phosphor in sewage.
Background technology
Low temperature is because the reduction of temperature has influenced microbial growth and metabolism to the reason that the biological treatment ability produces great effect.Though polyP bacteria is the low temperature psychrotroph, in temperature decline process, the phosphorus of releasing of polyP bacteria also is affected with suction phosphorus speed,, thus cause sewage work's effluent quality in winter to descend.Therefore need a kind of bacterial strain of removing phosphor in sewage of under coldcondition, strengthening badly.
Summary of the invention
The objective of the invention is to overcome the existing not good deficiency of bacterial strain low temperature dephosphorization effect, a kind of golden yellow bacillus of removing phosphor in sewage is provided.
Second purpose of the present invention provides a kind of isolation cultivation method of removing the golden yellow bacillus of phosphor in sewage.
The 3rd purpose of the present invention provides a kind of purposes of removing the golden yellow bacillus of phosphor in sewage.
Technical scheme of the present invention is summarized as follows:
A kind of low temperature goes down except that the deep yellow phosphorus CGMCC No.5282 that gathers of the golden yellow bacillus of phosphor in sewage (Chryseobacterium sp.), and it has in the low-temperature aerobic condition to go down except that the ability of phosphor in sewage.
A kind of low temperature goes down and goes down except that the purposes of phosphor in sewage in the low-temperature aerobic condition except that the deep yellow phosphorus CGMCC No.5282 that gathers of the golden yellow bacillus of phosphor in sewage (Chryseobacterium sp.).
Low temperature is preferably 8～12 ℃.
Low temperature go down except that the golden yellow bacillus of phosphor in sewage isolation cultivation method, comprise the steps:
(1) rich phosphorus nutrient solution is put into lab scale sbr reactor device, sludge of sewage treatment plant is inoculated in the said rich phosphorus nutrient solution, at 8～12 ℃; Cultured continuously 20 days; The cycle of operation is 6 hours, stirs 2.5 hours aerobic 2.5 hours comprising anaerobism; Left standstill 0.5 hour, row's supernatant reaches and replenished new rich phosphorus nutrient solution totally 0.5 hour; It is 3000mg/L that run duration keeps sludge concentration; (2) get the mud that 10mL step (1) obtains, add in the 250ml Erlenmeyer flask that 90mL sterile purified water and 20～30 sterilization granulated glass spherees have been housed, vibration 10～30min makes bacterial cell fully be discharged in the supernatant; Leave standstill 20～40min, get the adding of 1mL supernatant and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing serial dilution as stated above, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get 1mL respectively and be inoculated in rich phosphorus solid medium, each extent of dilution is done 3 parallel appearance, numbering; Under the condition of aerobic, preserve moisture for 10 ℃ and cultivated for 2～3 weeks;
(4) choosing colony is big in the rich phosphorus solid medium flat board from step (3), single bacterium colony that form is different, and line separates once more in rich phosphorus solid medium flat board; Repeating plate streaking separates 3-4 time; Make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is; The deep yellow phosphorus that gathers of called after goes down except that the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage through being accredited as low temperature
Said rich phosphorus nutrient solution is to be made up of following component: CH
3COONa3H
2O 3.32g, KH
2PO
443.75mg, CaCl
22H
2O 25.68mg, KNO
3485.94mg, MgSO
47H
2O 91.26mg, 5mL Tris damping fluid and 2mL trace element; Adding distil water is to 1000mL, and regulating pH is 7.2;
Consisting of of said trace element solution: EDTA 5g, FeSO
47H
2O 5g, CuSO
45H
2O 1.6g, MnCl
24H
2O 5g, (NH
4)
6Mo
7O
24H
2O 50mg, H
3BO
350mg, KI 10mg and CoCl
26H
2O 50mg adding distil water is to 1000mL.
Said rich phosphorus solid medium is to be made up of following component: CH
3COONa3H
2O 3.32g, KH
2PO
443.75mg, CaCl
22H
2O 25.68mg, KNO
3485.94mg, MgSO
47H
2O 91.26mg, 5mL Tris damping fluid and 2mL trace element, agar 20g, adding distil water are to 1000mL, and regulating pH is 7.2;
Consisting of of said trace element solution: EDTA 5g, FeSO
47H
2O 5g, CuSO
45H
2O 1.6g, MnCl
24H
2O 5g, (NH
4)
6Mo
7O
24H
2O 50mg, H
3BO
350mg, KI 10mg and CoCl
26H
2O 50mg adding distil water is to 1000mL.
Experimental result shows: although receive the obvious inhibition of low temperature environment; Adding a kind of low temperature of the present invention goes down and gathers phosphorus CGMCC No.5282 at low temperatures except that the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage is deep yellow; Particularly under 8-12 ℃ condition, removal has tangible strengthening effect to phosphorus, and tp removal rate has improved 10%; What be superior to one-level A goes out water quality standard (water temperature≤12 ℃ time, the controlling index of one-level A standard is 0.5mg/L).
Description of drawings
Fig. 1 goes down except that the deep yellow domestication separation and Culture schema that gathers phosphorus CGMCC No.5282 of the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage for a kind of low temperature.
Fig. 2 is the deep yellow test that gathers phosphorus removal phosphorus.
Fig. 3 is the deep yellow phosphor bacterium authentication method flow process of gathering of golden yellow bacillus (Chryseobacterium sp.).
Embodiment
Through specific embodiment the present invention is further described below.
Embodiment 1
Low temperature go down except that the golden yellow bacillus of phosphor in sewage isolation cultivation method, comprise the steps:
(1) rich phosphorus nutrient solution is put into lab scale sbr reactor device, recorded in the said rich phosphorus nutrient solution of sludge seeding of village's WWT plant aeration tank in Tianjin, at 10 ℃; Cultured continuously 20 days; The cycle of operation is 6 hours, stirs 2.5 hours aerobic 2.5 hours comprising anaerobism; Left standstill 0.5 hour, row's supernatant reaches and replenished new rich phosphorus nutrient solution totally 0.5 hour; It is 3000mg/L that run duration keeps sludge concentration;
(2) get the mud that 10mL step (1) obtains, add in the 250ml Erlenmeyer flask that 90mL sterile purified water and 30 sterilization granulated glass spherees have been housed, vibration 20min makes bacterial cell fully be discharged in the supernatant; Leave standstill 30min, get the adding of 1mL supernatant and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing serial dilution as stated above, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get 1mL respectively and be inoculated in rich phosphorus solid medium, each extent of dilution is done 3 parallel appearance, numbering; Under the condition of aerobic, preserve moisture for 10 ℃ and cultivated for 3 weeks;
(4) choosing colony is big in the rich phosphorus solid medium flat board from step (3), single bacterium colony that form is different, and line separates once more in rich phosphorus solid medium flat board; Repeating plate streaking separates 4 times; Make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is; The deep yellow phosphorus that gathers of called after goes down except that the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage through being accredited as low temperature
Embodiment 2
Rich phosphorus nutrient solution is to be made up of following component:
CH
3COONa3H
2O 3.32g, KH
2PO
443.75mg, CaCl
22H
2O 25.68mg, KNO
3485.94mg, MgSO
47H
2O 91.26mg, 5mL Tris damping fluid and 2mL trace element; Adding distil water is to 1000mL, and regulating pH is 7.2;
Consisting of of said trace element solution: EDTA 5g, FeSO
47H
2O 5g, CuSO
45H
2O 1.6g, MnCl
24H
2O 5g, (NH
4)
6Mo
7O
24H2O 50mg, H
3BO
350mg, KI 10mg and CoCl
26H
2O 50mg adding distil water is to 1000mL.
Embodiment 3
Rich phosphorus solid medium is to be made up of following component:
CH
3COONa3H
2O 3.32g, KH
2PO
443.75mg, CaCl
22H
2O 25.68mg, KNO
3485.94mg, MgSO
47H
2O 91.26mg, 5mL Tris damping fluid and 2mL trace element, agar 20g, adding distil water are to 1000mL, and regulating pH is 7.2;
Consisting of of said trace element solution: EDTA 5g, FeSO
47H
2O 5g, CuSO
45H
2O 1.6g, MnCl
24H
2O 5g, (NH
4)
6Mo
7O
24H
2O 50mg, H
3BO
350mg, KI10mg and CoCl
26H
2O 50mg adding distil water is to 1000mL.
Embodiment 4
Golden yellow bacillus of the present invention (Chryseobacterium sp.) has following Microbiological Characteristics:
1. morphological characteristic
Examine under a microscope, for shaft-like, long 2.7 μ m, wide 0.4 μ m; On rich phosphorus solid medium, form yellow bacterium colony.
2. cultivate and learn characteristic
Under 10 ℃, when in rich phosphorus solid medium, cultivating, Chryseobacterium sp. bacterial strain of the present invention has following characteristic.
Surface drying, the edge is irregular, thalline in pelletized form, color is yellow.
3. physiological property
Aerobic condition goes down except that the gram positive bacterium of phosphor in sewage, and catalase is negative, methyl red test is positive, hydrolyzed starch, liquefy gelatin, generation indoles, growth pH value are 6～8,5～30 ℃ of growth temperatures.
| Gram | Catalase | Methyl red | The starch hydrolysis | The gelatin test | Indole test | pH | Temperature |
| G + | - | + | + | + | + | 6～8 | 5～30 |
The 16s Sequence Identification of bacterium of the present invention
16SrDNA is the sequence that bacterium class prokaryotic organism are very guarded in very long evolutionary process; Playing the part of important role in the taxonomy of mikrobe in modern times; As the most frequently used phyletic evolution tagged molecule of bacterial flora structural analysis, it is more efficient and convenient to carry out structural analysis of microbial community with 16S rDNA.
Carrying out Bacteria Identification with the 16s sequence is undertaken by Fig. 3.
Beef-protein medium among Fig. 3: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium-chlor 5g, adding distil water are to 1000mL, and pH 7.0～7.2
The LB substratum: tryptone 10g, yeast extract 5g, NaCl 10g, adding distil water is to 1000ml.
The 16s sequence that the present invention records is as follows:
ACGCTAGCGGGAGGCCTAACACATGCAAGCCGAGCGGTAGAGATTCTTCGGGATCTTGAGAGCGGCG
TACGGGTGCGGAACACGTGTGCAACCTGCCTTTATCTGGGAGATAGCCTTTCGAAAGGAAGATTAATA
TCCCATAATATATTGACTGGCATCAGTTGATATTGAAAGCTCCGGCGGATAGAGATGGGCACGCGCAAG
ATTAGATAGTTGGTGAGGTAACGGCTCACCAAGTCAGTGATCTTTAGGGGGCCTGAGAGGGTGATCCC
CCACACTGGTACTGAGACACGGACCAGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGG
GTGCAAGCCTGATCCAGCCATCCCGCGTGAAGGACGACGGCCCTATGGGTTGTAAACTTCTTTTGTAT
AGGGATAAACCTTTCCACGTGTGGAAAGCTGAAGGTACTATACGAATAAGCACCGGCTAACTCCGTGC
CAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTAGGC
GGATCTGTAAGTCAGTGGTGAAATCTCACAGCTTAACTGTGAAACTGCCATTGATACTGCAGGTCTTG
AGTAAGGTAGAAGTGGCTGGAATAAGTAGTGTAGCGGTGAAATGCATAGATATTACTTAGAACACCAA
TTGCGAAGGCAGGTCACTATGTCTTAACTGACGCTGATGGACGAAAGCGTGGGGAGCGAACAGGATT
AGATACCCTGGTAGTCCACGCCGTAAACGATGCTAACTCGTTTTTGGGGCCTAGGCTTCAGAGACTAA
GCGAAAGTGATAAGTTAGCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGG
GGCCCGCACAAGCGGTGGATTATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCAAGGCTTAAA
TGGGAATTGATGGGTTTAGAAATAGACCGTCCTTCGGGCAATTTTCAAGGTGCTGCATGGTTGTCGTC
AGCTCGTGCCGTGAGGTGTTAGGTTAAGTCCTGCAACGAGCGCAACCCCTGTCACTAGTTGCCATCAT
TAAGTTGGGGACTCTAGTGAGACTGCCTACGCAAGTAGAGAGGAAGGTGGGGATGACGTCAAATCAT
CACGGCCCTTACGCCTTGGGCCACACACGTAATACAATGGCCGGTACAGAGGGCAGCTACACAGCGA
TGTGATGCAAATCTCGAAAGCCGGTCTCAGTTCGGATTGGAGTCTGCAACTCGACTCTATGAAGCTGG
AATCGCTAGTAATCGCGCATCAGCCATGGCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG
TCAAGCCATGGAAGTCTGGGGTACCTGAAGTCGGTGACCGTAACAGGAGCTGCCTAGGGTAAAACAG
GTAACTAG
Learn characteristic according to mentioned microorganism; The deep yellow phosphorus that gathers of bacterial strain called after of the present invention; The classification golden yellow bacillus of called after (Chryseobacterium sp.) is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 22nd, 2011, and deposit number is CGMCC No.5282, and the preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica, and survived.
The deep yellow degradation effect that gathers phosphorus to phosphor in sewage
In the beaker of 1L, add the rich phosphorus nutrient solution of 500m L (initial phosphorus concentration is 10mg/L), adds an amount of deep yellow polyP bacteria, maintenance pH is 7.2, its degraded situation of measuring 6 hours to phosphorus, the result is as shown in Figure 2.
Can be found out that by Fig. 2 through 6 hours degraded, phosphorus concentration was 0.45mg/L in the solution, phosphorus is removed basically, and clearance is 95.5%, proves that the deep yellow phosphorus that gathers among the present invention has good removal ability to phosphorus.
Embodiment 6
Low temperature go down except that the golden yellow bacillus of phosphor in sewage isolation cultivation method, comprise the steps:
(1) rich phosphorus nutrient solution is put into lab scale sbr reactor device, recorded in the said rich phosphorus nutrient solution of sludge seeding of village's WWT plant aeration tank in Tianjin, at 8 ℃; Cultured continuously 20 days; The cycle of operation is 6 hours, stirs 2.5 hours aerobic 2.5 hours comprising anaerobism; Left standstill 0.5 hour, row's supernatant reaches and replenished new rich phosphorus nutrient solution totally 0.5 hour; It is 3000mg/L that run duration keeps sludge concentration;
(2) get the mud that 10mL step (1) obtains, add in the 250ml Erlenmeyer flask that 90mL sterile purified water and 30 sterilization granulated glass spherees have been housed, vibration 30min makes bacterial cell fully be discharged in the supernatant; Leave standstill 40min, get the adding of 1mL supernatant and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing serial dilution as stated above, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get 1mL respectively and be inoculated in rich phosphorus solid medium, each extent of dilution is done 3 parallel appearance, numbering; Under the condition of aerobic, preserve moisture for 10 ℃ and cultivated for 2 weeks;
(4) choosing colony is big in the rich phosphorus solid medium flat board from step (3); Single bacterium colony that form is different, line separates once more in rich phosphorus solid medium flat board, repeats plate streaking and separates 3 times; Make the homomorphosis of bacterium colony in microscope on the flat board; Single, big or small similar single bacterium colony that is down except that the golden yellow bacillus (Chryseobacteriumsp.) of phosphor in sewage through being accredited as low temperature
Embodiment 7
Low temperature goes down except that the isolation cultivation method of the golden yellow bacillus of phosphor in sewage, comprises the steps:
(1) rich phosphorus nutrient solution is put into lab scale sbr reactor device, recorded in the said rich phosphorus nutrient solution of sludge seeding of village's WWT plant aeration tank in Tianjin, at 12 ℃; Cultured continuously 20 days; The cycle of operation is 6 hours, stirs 2.5 hours aerobic 2.5 hours comprising anaerobism; Left standstill 0.5 hour, row's supernatant reaches and replenished new rich phosphorus nutrient solution totally 0.5 hour; It is 3000mg/L that run duration keeps sludge concentration;
(2) get the mud that 10mL step (1) obtains, add in the 250ml Erlenmeyer flask that 90mL sterile purified water and 20 sterilization granulated glass spherees have been housed, vibration 10min makes bacterial cell fully be discharged in the supernatant; Leave standstill 20min, get the adding of 1mL supernatant and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing serial dilution as stated above, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get 1mL respectively and be inoculated in rich phosphorus solid medium, each extent of dilution is done 3 parallel appearance, numbering; Under the condition of aerobic, preserve moisture for 10 ℃ and cultivated for 3 weeks;
(4) choosing colony is big in the rich phosphorus solid medium flat board from step (3); Single bacterium colony that form is different, line separates once more in rich phosphorus solid medium flat board, repeats plate streaking and separates 4 times; Make the homomorphosis of bacterium colony in microscope on the flat board; Single, big or small similar single bacterium colony that is down except that the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage through being accredited as low temperature
Experiment showed, that embodiment 6 and embodiment 7 low temperature go down the effect of good removal phosphorus is also arranged except that the golden yellow bacillus of the isolation cultivation method separation and Culture of the golden yellow bacillus of phosphor in sewage, clearance is respectively 94.4% and 95.0%.
Claims (6)
1. a low temperature goes down except that the deep yellow phosphorus CGMCC No.5282 that gathers of the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage, and it has in the low-temperature aerobic condition to go down except that the ability of phosphor in sewage.
2. a kind of low temperature of claim goes down down except that the purposes of phosphor in sewage in the low-temperature aerobic condition except that the deep yellow phosphorus CGMCC No.5282 that gathers of the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage.
3. purposes according to claim 2 is characterized in that said low temperature is 8～12 ℃.
The low temperature of claim 1 go down except that the golden yellow bacillus of phosphor in sewage isolation cultivation method, it is characterized in that comprising the steps:
(1) rich phosphorus nutrient solution is put into lab scale sbr reactor device, sludge of sewage treatment plant is inoculated in the said rich phosphorus nutrient solution, at 8～12 ℃; Cultured continuously 20 days; The cycle of operation is 6 hours, stirs 2.5 hours aerobic 2.5 hours comprising anaerobism; Left standstill 0.5 hour, row's supernatant reaches and replenished new rich phosphorus nutrient solution totally 0.5 hour; It is 3000mg/L that run duration keeps sludge concentration;
(2) get the mud that 10mL step (1) obtains, add in the 250ml Erlenmeyer flask that 90mL sterile purified water and 20～30 sterilization granulated glass spherees have been housed, vibration 10～30min makes bacterial cell fully be discharged in the supernatant; Leave standstill 20～40min, get the adding of 1mL supernatant and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing serial dilution as stated above, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get 1mL respectively and be inoculated in rich phosphorus solid medium, each extent of dilution is done 3 parallel appearance, numbering; Under the condition of aerobic, preserve moisture for 10 ℃ and cultivated for 2～3 weeks;
(4) choosing colony is big in the rich phosphorus solid medium flat board from step (3), single bacterium colony that form is different, and line separates once more in rich phosphorus solid medium flat board; Repeating plate streaking separates 3-4 time; Make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is; The deep yellow phosphorus that gathers of called after goes down except that the golden yellow bacillus (Chryseobacterium sp.) of phosphor in sewage through being accredited as low temperature
5. low temperature according to claim 4 go down except that the golden yellow bacillus of phosphor in sewage isolation cultivation method, it is characterized in that said rich phosphorus nutrient solution is for to be made up of following component: CH
3COONa3H
2O 3.32g, KH
2PO
443.75mg, CaCl
22H
2O 25.68mg, KNO
3485.94mg, MgSO
47H
2O 91.26mg, 5mL Tris damping fluid and 2mL trace element; Adding distil water is to 1000mL, and regulating pH is 7.2;
Consisting of of said trace element solution: EDTA 5g, FeSO
47H
2O 5g, CuSO
45H
2O 1.6g, MnCl
24H
2O5g, (NH
4)
6Mo
7O
24H
2O 50mg, H
3BO
350mg, KI 10mg and CoCl
26H
2O 50mg adding distil water is to 1000mL.
6. low temperature according to claim 4 go down except that the golden yellow bacillus of phosphor in sewage isolation cultivation method, it is characterized in that said rich phosphorus solid medium is for to be made up of following component: CH
3COONa3H
2O 3.32g, KH
2PO
443.75mg, CaCl
22H
2O25.68mg, KNO
3485.94mg, MgSO
47H
2O 91.26mg, 5mL Tris damping fluid and 2mL trace element, agar 20g, adding distil water are to 1000mL, and regulating pH is 7.2;
Consisting of of said trace element solution: EDTA 5g, FeSO
47H
2O 5g, CuSO
45H
2O 1.6g, MnCl
24H
2O 5g, (NH
4)
6Mo
7O
24H
2O 50mg, H
3BO
350mg, KI 10mg and CoCl
26H
2O 50mg adding distil water is to 1000mL.
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| CN105236572A (en) * | 2015-10-27 | 2016-01-13 | 深圳德玛克环保科技有限公司 | Method for culturing phosphorus-accumulating particle sludge through low-temperature SBR technology and application |
| CN105236572B (en) * | 2015-10-27 | 2017-11-24 | 深圳信息职业技术学院 | The method of low temperature SBR process culture polyphosphate particle sludge and application |
| CN106701640A (en) * | 2017-02-17 | 2017-05-24 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | New strain of producing strain XJ2 of natural yellow pigment and preparation and application thereof |
| CN108658245A (en) * | 2018-04-30 | 2018-10-16 | 桂林理工大学 | A kind of preparation method of processing lead zinc waste water microbial flocculant |
| CN114703078A (en) * | 2020-12-16 | 2022-07-05 | 沈阳恩柽研究院有限公司 | Chryseobacterium indolerum and application thereof |
| CN114703078B (en) * | 2020-12-16 | 2023-08-29 | 沈阳恩柽研究院有限公司 | Indolyl golden fungus and its application |
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