CN102443557B - Strain for removing nitrite nitrogen in sewage under low-temperature condition and separation culture method - Google Patents
Strain for removing nitrite nitrogen in sewage under low-temperature condition and separation culture method Download PDFInfo
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- CN102443557B CN102443557B CN2011104162904A CN201110416290A CN102443557B CN 102443557 B CN102443557 B CN 102443557B CN 2011104162904 A CN2011104162904 A CN 2011104162904A CN 201110416290 A CN201110416290 A CN 201110416290A CN 102443557 B CN102443557 B CN 102443557B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention discloses a strain for removing nitrite nitrogen in sewage under the low-temperature condition and a separation culture method. A strain (Perlucidibaca piscinae) nitrate CGMCC No.5281 for removing nitrite nitrogen in sewage under the low-temperature condition has the capability of removing the nitrite nitrogen in the sewage under the low-temperature aerobic condition. Experiment results show that although the effect is obviously inhibited by the low-temperature environment, the strain (Perlucidibaca piscinae) nitrate CGMCC No.5281 for removing the nitrite nitrogen in the sewage under the low-temperature condition is added, and the obvious enhancement effect is realized on the nitrate removal in the sewage at a low temperature and particularly under the condition of 8 to 12 DEG C. Compared with that in sewage not inoculated with the strain, the nitrite removal rate of the sewage inoculated with the strain is improved by about 10 percent.
Description
Technical field
The present invention relates to a strain and remove bacterium of Nitrite In Polluted Water nitrate nitrogen and uses thereof.
Background technology
The reason that low temperature produces great effect to the biological treatment ability is because the reduction of temperature has affected microbial growth and metabolism and made that change has occured microbial population in the Sewage treatment systems.In the drop in temperature process, the metabolic activity of mesophilic bacteria descends gradually, just lose activity during to certain temperature, and be suitable for low temperature environment psychrophile quantity since self physiological property and the restraining effect of various ecological factors, quantitatively can't reach the status of dominant microflora, thereby cause sewage work's effluent quality in winter to descend.Therefore need badly a kind ofly can go down except the bacterial strain of sewage Nitrite Nitrogen in the low-temperature aerobic condition.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide a kind of bacterial strain (Perlucidibaca piscinae) nitre of removing nitrite nitrogen in sewage under low-temperature condition saturating.
Bacterial strain (Perlucidibaca piscinae) nitre that second purpose of the present invention provides a kind of removing nitrite nitrogen in sewage under low-temperature condition goes down except the purposes of sewage Nitrite Nitrogen in the low-temperature aerobic condition thoroughly.
The 3rd purpose of the present invention provides a kind of isolation cultivation method of bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition.
Technical scheme of the present invention is summarized as follows:
The saturating CGMCC No.5281 of a kind of bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae) nitre, it has in the low-temperature aerobic condition to go down except the ability of sewage Nitrite Nitrogen.
The saturating CGMCC No.5281 of a kind of bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae) nitre goes down except the purposes of sewage Nitrite Nitrogen in the low-temperature aerobic condition.Low temperature is preferably 8-12 ℃.
The isolation cultivation method of the bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition, comprise the steps: (1) with the sludge seeding of 10～20mL sewage disposal plant aeration tank to the Erlenmeyer flask that 250mL Nitrate bacteria nutrient solution is housed, nitrogen concentration is 50mg/L in the Sodium Nitrite in the described Nitrate bacteria nutrient solution, at 8～12 ℃, shaking speed is to tame in 120～140rpm shaking table, when treating that the nitrite nitrogen degradation rate reaches 80～90%, leave standstill, remove supernatant liquor, with the sludge seeding of Erlenmeyer flask bottom to being equipped with in 250mL the second Nitrate bacteria nutrient solution, nitrite nitrogen concentration in described the second Nitrate bacteria nutrient solution is 150mg/L, at 8～12 ℃, in being the shaking table of 120～140rpm, rotating speed tames, when treating that the nitrite nitrogen degradation rate reaches 80～90%, namely obtain containing the Nitrate bacteria nutrient solution of low temperature resistant bacterium;
(2) get the nutrient solution that 10mL step (1) obtains, add in the 250mL Erlenmeyer flask that 90mL sterile purified water and 20～30 sterilization granulated glass spherees have been housed, vibration 10～30min makes bacterial cell fully be discharged in the supernatant liquor; Leave standstill 20～40min, get the adding of 1mL supernatant liquor and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing as stated above serial dilution, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get respectively 1mL and be inoculated in the Nitrate bacteria solid medium, each extent of dilution is done 3 Duplicate Samples, the numbering; 10 ℃ of moisturizing 2～3 weeks of cultivation under the condition of aerobic;
(4) choosing colony is large in the Nitrate bacteria solid medium flat board from step (3), single bacterium colony that form is different, line separates again in Nitrate bacteria solid medium flat board, repeating plate streaking separates 3-4 time, make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is, called after nitre is saturating, the bacterial strain through being accredited as removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae).
Described Nitrate bacteria nutrient solution is comprised of following component: NaNO
20.25g, sodium acetate 2g, MgSO
47H
2O 0.05g, K
2HPO
40.2g, NaCl 0.12g, MnSO
44H
2O 0.01g, FeSO
40.01g, adding distil water is to 1000m L.
Described the second Nitrate bacteria nutrient solution is comprised of following component: NaNO
20.74g, sodium acetate 2g, MgSO
47H
2O0.05g, K
2HPO
40.2g, NaCl 0.12g, MnSO
44H
2O 0.01g, FeSO
40.01g, adding distil water is to 1000mL.
Described Nitrate bacteria solid medium is comprised of following component: NaNO
20.5g, sodium acetate 2g, MgSO
47H
2O0.05g, K
2HPO
40.2g, NaCl 0.12g, MnSO
44H
2O 0.01g, FeSO
40.01g, agar 20g, adding distil water be to 1000m L.
Experimental result shows: although be subject to the obvious inhibition of low temperature environment, add the saturating CGMCC No.5281 of bacterial strain (Perlucidibaca piscinae) nitre of a kind of removing nitrite nitrogen in sewage under low-temperature condition of the present invention, at low temperatures, particularly under 8-12 ℃ condition, the Nitrite In Polluted Water nitrate removal there is obvious strengthening effect, inoculate bacterial strain of the present invention and compare with the sewage of not inoculating bacterium, the nitrite clearance has improved and has been about 10%.
Description of drawings
Fig. 1 is that the low-temperature aerobic condition is gone down except a kind of domestication separation and Culture schema of removing the saturating CGMCC No.5281 of bacterial strain (Perlucidibaca piscinae) nitre of Nitrite In Polluted Water nitrate nitrogen of sewage Nitrite Nitrogen.
Fig. 2 is that bacterial strain (Perlucidibaca piscinae) nitre is removed test to nitrite nitrogen thoroughly.
Fig. 3 is the saturating authentication method flow process of bacterial strain (Perlucidibaca piscinae) nitre.
Embodiment
The present invention is further illustrated below by specific embodiment.
Embodiment 1
The saturating isolation cultivation method of the bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae) nitre comprises the steps:
(1) with the sludge seeding of 15mL Tianjin discipline village sewage disposal plant aeration tank to the Erlenmeyer flask that 250mL Nitrate bacteria nutrient solution is housed, nitrogen concentration is 50mg/L in the Sodium Nitrite in the described Nitrate bacteria nutrient solution, at 10 ℃, shaking speed is to tame in the 130rpm shaking table, when treating that the nitrite nitrogen degradation rate reaches 90%, leave standstill, remove supernatant liquor, with the sludge seeding of Erlenmeyer flask bottom to being equipped with in 250mL the second Nitrate bacteria nutrient solution, nitrite nitrogen concentration in described the second Nitrate bacteria nutrient solution is 150mg/L, at 10 ℃, in being the shaking table of 130rpm, rotating speed tames, when treating that the nitrite nitrogen degradation rate reaches 90%, namely obtain containing the Nitrate bacteria nutrient solution of low temperature resistant bacterium;
(2) get the nutrient solution that 10mL step (1) obtains, add in the 250mL Erlenmeyer flask that 90mL sterile purified water and 30 sterilization granulated glass spherees have been housed, vibration 20min makes bacterial cell fully be discharged in the supernatant liquor; Leave standstill 30min, get the adding of 1mL supernatant liquor and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing as stated above serial dilution, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get respectively 1mL and be inoculated in the Nitrate bacteria solid medium, each extent of dilution is done 3 Duplicate Samples, the numbering; 10 ℃ under the condition of aerobic moisturizing cultivated for 3 weeks;
(4) choosing colony is large in the Nitrate bacteria solid medium flat board from step (3), single bacterium colony that form is different, line separates again in Nitrate bacteria solid medium flat board, repeating plate streaking separates 3 times, make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is, called after nitre is saturating, the bacterial strain through being accredited as removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae).
The Nitrate bacteria nutrient solution that adopts among each embodiment is comprised of following component: NaNO
20.25g, sodium acetate 2g, MgSO
47H
2O 0.05g, K
2HPO
40.2g, NaCl 0.12g, MnSO
44H
2O 0.01g, FeSO
40.01g, adding distil water is to 1000m L.
The second Nitrate bacteria nutrient solution is comprised of following component: NaNO
20.74g, sodium acetate 2g, MgSO
47H
2O 0.05g, K
2HPO
40.2g, NaCl 0.12g, MnSO
44H
2O 0.01g, FeSO
40.01g, adding distil water is to 1000m L.
The Nitrate bacteria solid medium is comprised of following component: NaNO
20.5g, sodium acetate 2g, MgSO
47H
2O 0.05g, K
2HPO
40.2g, NaCl 0.12g, MnSO
44H
2O 0.01g, FeSO
40.01g, agar 20g, adding distil water be to 1000mL.
A kind of bacterial strain (Perlucidibaca piscinae) nitre of removing the Nitrite In Polluted Water nitrate nitrogen of the present invention is saturating, has following Microbiological Characteristics:
1. morphological characteristic
Examine under a microscope, for shaft-like, long 1.6 μ m, wide 0.35 μ m; Form transparent bacterium colony at the Nitrate bacteria solid medium.
2. cultivate and learn characteristic
Under 10 ℃, when cultivating in the Nitrate bacteria solid medium, (Perlucididaca piscinae) of the present invention bacterial strain has following characteristic.
Surface drying, neat in edge, individual small flat, thalline is transparent.
3. physiological property
Aerobic condition goes down except the gram negative bacterium of sewage Nitrite Nitrogen, and catalase is negative, methyl red test is negative, hydrolyzed starch, liquefy gelatin, edwardsiella hoshinae, growth pH value are 6～8,5～30 ℃ of growth temperatures.
The 16s Sequence Identification of bacterium of the present invention
16SrDNA is bacterium class prokaryotic organism very conservative sequences in very long evolutionary process, in the taxonomy of modern microorganism, playing the part of important role, as the most frequently used phyletic evolution tagged molecule of bacterial flora structural analysis, it is more efficient and convenient to carry out structural analysis of microbial community with 16S rDNA.
Carrying out the Bacteria Identification flow process with the 16s sequence is undertaken by Fig. 3.
Beef-protein medium among Fig. 3 forms: extractum carnis 3g, and peptone 10g, sodium-chlor 5g, adding distil water are to 1000mL, and pH 7.0～7.2.
The LB substratum: tryptone 10g, yeast extract 5g, NaCl 10g, adding distil water is to 1000mL.
The 16s sequence that the present invention records is as follows:
ACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGACTTCGGTCCTAGCGGCGAACGGGTGA
GGAATGCTTGGGAATCTGCCCAGTAGTGGGGGATAACGTTTCGAAAGGAACGCTAATACCGCATACGT
CCTACGGGAGAAAGGGGGGGATCTTCGGACCTCTCGCTATTGGATGAGCCCAAGTCGGATTAGCTAGT
TGGTGGGGTAAAGGCCCACCAAGGCGACGATCCGTAGCTGGTCTGAGAGGATGATCAGCCACACTGG
AACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGC
CTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTAAGCAGGGAGGAA
TGGCTCTTGGTTAATAACCTTGAGCAGTGACGTTACCTGCAGAATAAGCACCGGCTAACTCTGTGCCA
GCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGCG
GTTACGTAAGTTGGGTGTGAAATCCCCGGGCTCAACCTGGGAATTGCACTCAAAACTGCGTGGCTAG
AGTGTGGGAGAGGAAGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGATATCTGGAGGAATACCG
ATGGCGAAGGCAGCCTTCTGGCCTAACACTGACGCTGAGGTGCGAAAGCATGGGGAGCAAACAGGA
TTAGATACCCTGGTAGTCCATGCCGTAAACGATGTCTACTAGCCGTTGGGGGACTTGATCCTTTAGTGG
CGCAGCTAACGCATTAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGA
CGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGCC
TTGACATGTCCGGAATCCTGCAGAGATGCGGGAGTGCCTTCGGGAATCGGAACACAGGTGCTGCATG
GCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGT
TGCCAGCACGTAATGGTGGGAACTCTAGGGAGACTGCCGGTGACAAACCGGAGGAAGGCGGGGACG
ACGTCAAGTCATCATGGCCCTTACGGCCAGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTT
GCTAGCCCGCGAGGGTACGCCAATCTCAAAAAGCCGATCGTAGTCCGGATTGGAGTCTGCAACTCGA
CTCCATGAAGTCGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGCCTTG
TACACACCGCCCGTCACACCATGGGAGTTTACTGCACCAGAAGTAGGTAGCTTAACCGCAAGGAGGG
CGCTTACCACGGTGTGGTCAATGACTGGG
Learn characteristic according to mentioned microorganism, bacterial strain called after nitre of the present invention is saturating, Classification And Nomenclature is that bacterial strain (Perlucidibaca piscinae) has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 22nd, 2011, deposit number is CGMCC No.5281, the preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, and survived.
Embodiment 2 nitre are thoroughly to the degradation effect of nitrite nitrogen
In the beaker of 1L, add 500m L Nitrate bacteria nutrient solution, initial nitrite nitrogen concentration is adjusted into 50mg/L, it is saturating to add nitre of the present invention, connecing three rings nitre of the present invention from the Nitrate bacteria solid plate penetrates the above-mentioned Nitrate bacteria nutrient solution, keeping pH is 7.5, measures 72 hours nitre thoroughly to the degraded situation of nitrite nitrogen, the results are shown in Figure 2.
As seen from Figure 2, the degraded through 72 hours, nitrogen concentration of nitrite solution is 6.6mg/L, and nitrite nitrogen is removed substantially, and clearance is 86.8%, proves that the nitre among the present invention has good removal ability to nitrite nitrogen thoroughly.
Embodiment 3
The saturating isolation cultivation method of the bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae) nitre comprises the steps:
(1) with the sludge seeding of 10mL Tianjin discipline village sewage disposal plant aeration tank to the Erlenmeyer flask that 250mL Nitrate bacteria nutrient solution is housed, nitrogen concentration is 50mg/L in the Sodium Nitrite in the described Nitrate bacteria nutrient solution, at 8 ℃, shaking speed is to tame in the 140rpm shaking table, when treating that the nitrite nitrogen degradation rate reaches 80%, leave standstill, remove supernatant liquor, with the sludge seeding of Erlenmeyer flask bottom to being equipped with in 250mL the second Nitrate bacteria nutrient solution, nitrite nitrogen concentration in described the second Nitrate bacteria nutrient solution is 150mg/L, at 8 ℃, in being the shaking table of 140rpm, rotating speed tames, when treating that the nitrite nitrogen degradation rate reaches 80%, namely obtain containing the Nitrate bacteria nutrient solution of low temperature resistant bacterium;
(2) get the nutrient solution that 10mL step (1) obtains, add in the 250mL Erlenmeyer flask that 90mL sterile purified water and 20 sterilization granulated glass spherees have been housed, vibration 30min makes bacterial cell fully be discharged in the supernatant liquor; Leave standstill 20min, get the adding of 1mL supernatant liquor and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing as stated above serial dilution, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get respectively 1mL and be inoculated in the Nitrate bacteria solid medium, each extent of dilution is done 3 Duplicate Samples, the numbering; 10 ℃ under the condition of aerobic moisturizing cultivated for 2 weeks;
(4) choosing colony is large in the Nitrate bacteria solid medium flat board from step (3), single bacterium colony that form is different, line separates again in Nitrate bacteria solid medium flat board, repeating plate streaking separates 4 times, make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is, called after nitre is saturating, through being accredited as removing nitrite nitrogen in sewage under low-temperature condition bacterial strain (Perlucidibaca piscinae).
Embodiment 4
The saturating isolation cultivation method of the bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition (Perlucidibaca piscinae) nitre comprises the steps:
(1) with the sludge seeding of 20mL Tianjin discipline village sewage disposal plant aeration tank to the Erlenmeyer flask that 250mL Nitrate bacteria nutrient solution is housed, nitrogen concentration is 50mg/L in the Sodium Nitrite in the described Nitrate bacteria nutrient solution, at 12 ℃, shaking speed is to tame in the 120rpm shaking table, when treating that the nitrite nitrogen degradation rate reaches 90%, leave standstill, remove supernatant liquor, with the sludge seeding of Erlenmeyer flask bottom to being equipped with in 250mL the second Nitrate bacteria nutrient solution, nitrite nitrogen concentration in described the second Nitrate bacteria nutrient solution is 150mg/L, at 12 ℃, in being the shaking table of 120rpm, rotating speed tames, when treating that the nitrite nitrogen degradation rate reaches 90%, namely obtain containing the Nitrate bacteria nutrient solution of low temperature resistant bacterium;
(2) get the nutrient solution that 10mL step (1) obtains, add in the 250mL Erlenmeyer flask that 90mL sterile purified water and 30 sterilization granulated glass spherees have been housed, vibration 10min makes bacterial cell fully be discharged in the supernatant liquor; Leave standstill 40min, get the adding of 1mL supernatant liquor and be equipped with in the test tube of 9mL sterile distilled water, obtain 10
-2Bacteria suspension, take turns doing as stated above serial dilution, accomplish 10 always
-8Till;
(3) choosing gradient is 10
-5～10
-8Bacteria suspension, get respectively 1mL and be inoculated in the Nitrate bacteria solid medium, each extent of dilution is done 3 Duplicate Samples, the numbering; 10 ℃ under the condition of aerobic moisturizing cultivated for 3 weeks;
(4) choosing colony is large in the Nitrate bacteria solid medium flat board from step (3), single bacterium colony that form is different, line separates again in Nitrate bacteria solid medium flat board, repeating plate streaking separates 3 times, make the homomorphosis of bacterium colony in microscope on the flat board, single, big or small similar single bacterium colony that is, called after nitre is saturating, through being accredited as removing nitrite nitrogen in sewage under low-temperature condition bacterial strain (Perlucidibaca piscinae).
Experiment showed, that embodiment 3 and embodiment 4 separation and Culture remove the effect that the bacterial strain (Perlucidibaca piscinae) of Nitrite In Polluted Water nitrate nitrogen also has good degrading nitrite nitrogen, clearance is respectively 86.1% and 86.4%.
Sequence table
＜110〉pioneering municipal Genie et Environnement of Aiwa (Tianjin)
＜120〉bacterial strain of removing nitrite nitrogen in sewage under low-temperature condition and isolation cultivation method
<130>
<160>1
<170>PatentIn?version?3.3
<210>1
<211>1439
<212>DNA
<213>Perlucidibaca?piscinae
<400>1
acgctggcgg?caggcttaac?acatgcaagt?cgagcgggga?cttcggtcct?agcggcgaac60
gggtgaggaa?tgcttgggaa?tctgcccagt?agtgggggat?aacgtttcga?aaggaacgct120
aataccgcat?acgtcctacg?ggagaaaggg?ggggatcttc?ggacctctcg?ctattggatg180
agcccaagtc?ggattagcta?gttggtgggg?taaaggccca?ccaaggcgac?gatccgtagc240
tggtctgaga?ggatgatcag?ccacactgga?actgagacac?ggtccagact?cctacgggag300
gcagcagtgg?ggaatattgg?acaatgggcg?aaagcctgat?ccagccatgc?cgcgtgtgtg360
aagaaggcct?tcgggttgta?aagcacttta?agcagggagg?aatggctctt?ggttaataac420
cttgagcagt?gacgttacct?gcagaataag?caccggctaa?ctctgtgcca?gcagccgcgg480
taatacagag?ggtgcaagcg?ttaatcggaa?ttactgggcg?taaagcgcgc?gtaggcggtt540
acgtaagttg?ggtgtgaaat?ccccgggctc?aacctgggaa?ttgcactcaa?aactgcgtgg600
ctagagtgtg?ggagaggaag?gtagaattcc?aggtgtagcg?gtgaaatgcg?tagatatctg660
gaggaatacc?gatggcgaag?gcagccttct?ggcctaacac?tgacgctgag?gtgcgaaagc720
atggggagca?aacaggatta?gataccctgg?tagtccatgc?cgtaaacgat?gtctactagc780
cgttggggga?cttgatcctt?tagtggcgca?gctaacgcat?taagtagacc?gcctggggag840
tacggtcgca?agactaaaac?tcaaatgaat?tgacgggggc?ccgcacaagc?ggtggagcat900
gtggtttaat?tcgatgcaac?gcgaagaacc?ttacctggcc?ttgacatgtc?cggaatcctg960
cagagatgcg?ggagtgcctt?cgggaatcgg?aacacaggtg?ctgcatggct?gtcgtcagct1020
cgtgtcgtga?gatgttgggt?taagtcccgc?aacgagcgca?acccttgtcc?ttagttgcca1080
gcacgtaatg?gtgggaactc?tagggagact?gccggtgaca?aaccggagga?aggcggggac1140
gacgtcaagt?catcatggcc?cttacggcca?gggctacaca?cgtgctacaa?tggtcggtac?1200
aaagggttgc?tagcccgcga?gggtacgcca?atctcaaaaa?gccgatcgta?gtccggattg1260
gagtctgcaa?ctcgactcca?tgaagtcgga?atcgctagta?atcgcggatc?agaatgccgc1320
ggtgaatacg?ttcccgggcc?ttgtacacac?cgcccgtcac?accatgggag?tttactgcac1380
cagaagtagg?tagcttaacc?gcaaggaggg?cgcttaccac?ggtgtggtca?atgactggg1439
Claims (3)
- The bacterium of a removing nitrite nitrogen in sewage under low-temperature condition ( Perlucidibaca piscinae), called after nitre is saturating, and its microbial preservation number is CGMCC No.5281, and it has in the low-temperature aerobic condition to go down except the ability of sewage Nitrite Nitrogen.
- 2. the described bacterium of claim 1 goes down except the purposes of sewage Nitrite Nitrogen in the low-temperature aerobic condition.
- 3. purposes according to claim 2 is characterized in that described low temperature is 8-12 ℃.
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| CN101348300A (en) * | 2008-08-31 | 2009-01-21 | 华南理工大学 | Method for removing aquaculture water nitrite nitrogen by aerobic denitrification |
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