CN102426630A - Method for establishing electronic fingerprint database for expiratory gas of pneumonia patient - Google Patents
Method for establishing electronic fingerprint database for expiratory gas of pneumonia patient Download PDFInfo
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- CN102426630A CN102426630A CN2011103408358A CN201110340835A CN102426630A CN 102426630 A CN102426630 A CN 102426630A CN 2011103408358 A CN2011103408358 A CN 2011103408358A CN 201110340835 A CN201110340835 A CN 201110340835A CN 102426630 A CN102426630 A CN 102426630A
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Abstract
The invention provides a method for establishing an electronic nose and electronic fingerprint database for expiratory gas of a streptococcus pneumonia patient and a fingerprint database thereof by designing a simple and effective detection technology with an electronic nose aiming at the expiratory gas of a patient infected with streptococcus pneumoniae induced pneumonia and expiratory gas of healthy people. The technical method in the scheme is simpler, quicker, more efficient, more accurate and lower in cost; no pain is brought to the patient during sampling according to the scheme; a used instrument is easy to operate, skilful in volume and convenient to move and carry and has extremely high specificity and accuracy.
Description
Technical field
The present invention relates to a kind of method of setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air.
Background technology
The mixed gases that the human body expiratory air is made up of nitrogen, oxygen, carbon dioxide, water and VOC (VOCs).The metabolic portion of product of human body can be transported to lung via blood, gets into alveolar through gas exchange and appears in the breath.In recent years, the expiratory air detection receives increasing attention as the method for a kind of human physiological metabolism's of understanding process and disease condition.Compare with the blood testing of routine, it is fast, convenient and time-saving to human zero damage, detection speed that expiratory air detects great advantage.Detected VOC reaches thousands of kinds in the human body expiratory air at present.These compounds comprise alkane, alkene, group compounds of aldehydes and ketones, sulfocompound and nitrogen-containing compound etc.Wherein, Saturated alkane mainly produces through lipid peroxidation; The approach that unsaturated alkane produces is different, mainly results from the synthetic intermediate steps of cholesterol like isoprene, and most important acetone then derives from the decarboxylation of acetyl coenzyme A in the liver in the aldehyde ketone compound.
Discover VOCs and a lot of disease associations in the expiratory air at present.As being that the VOCs such as a series of alkane, isoprene and acetone of representative can reflect certain Human Physiology and pathologic condition with the pentane.Have and discover in the lung cancer crowd expiratory air have the content of 9 kinds of VOCs to be higher than healthy population; Pentane content is apparently higher than normal population in the acute asthma crowd breath; The alkane that methylates in the expiratory air is considered to the molecular marker of oxidative stress, and acetone can be used as the molecular marker of diabetes in the expiratory air.
The method and the technology that detect expiratory air VOCs at present have a lot, and major part depends on gas chromatography-mass spectrum logotype (GC-MS) technology and sets up.These class methods all must be accomplished through a plurality of steps of sample collecting, pre-treatment and separation detection.In recent years develop some new technical methods, these methods need not or only need very simple sample pre-treatments to detect, and also some can realize the real-time online of breath is detected.Electronic Nose (Electronic Nose; ENose) also claim artificial olfactory system; Be a kind of novel bionical detecting instrument of simulating biological sense of smell principle of work, form by chemical sensor array and algorithm for pattern recognition usually, can be used for detecting, analyze and differentiate complicated smell.On the structure, electric nasus system mainly is made up of gas sensor array, Signal Pretreatment unit and pattern recognition unit three parts.Electronic Nose shows wide application prospect in medical science, agricultural, food, industry, environmental protection and military affairs at present.
Streptococcus pneumonia pneumonia (Pneumococcal pneumonia) is by the caused pulmonary parenchyma property inflammation of streptococcus pneumonia (Streptococcus pneumoniae), accounts for the half the of community acquired pneumonia case.Streptococcus pneumonia is the Gram-positive facultative anaerobe; About 40%-50% healthy population upper respiratory tract carries streptococcus pneumonia, when Abwehrkraft des Koepers descends, after respiratory virus infection; Or chance chill; Or under the situation such as malnutritive or oldaged physically weak, streptococcus pneumonia will be through the mucous membrane defense system, comes downwards to lung and causes bacterial pneumonia from sticking the position.Streptococcus pneumonia also can cause relevant diseases such as tympanitis, bacteremia, meningitis.Streptococcus pneumonia is the most important pathogen that causes developing country and each age group crowd community acquired pneumonia of developed country.Worldwide, the annual death that is caused by streptococcus pneumoniae infection accounts for 9% of total population death toll.Compare with other bacteriums, streptococcus pneumonia is caused children and the elderly death of catching an illness more easily because of its aggressive.Even more serious is that streptococcus pneumonia has trend of rising gradually to multiple antibiotic drug resistance.The streptococcus pneumonia pneumonia is popular in all over the world, and mortality ratio is up to 30%-35%.2005, the data presentation that the World Health Organization (WHO) announces had the 1600000 people streptococcus pneumonia that dies of pneumonia every year approximately, comprised 70～children below 1,000,000 5 years old, and wherein majority is lived in developing country.The early diagnosis of streptococcus pneumonia pneumonia is to cure this disease and the key that reduces mortality ratio.The methods for clinical diagnosis success ratio of traditional streptococcus pneumonia pneumonia is low; And the factor that has many unfavorable patients or make a definite diagnosis; As being master's method with microbe culture and Gram; The diagnosis success ratio is low, process is numerous and diverse consuming time, with antigen or serum detect be master the method diagnostic fees with high, technical difficulty is big, recall rate is not high yet, with specific gene polymerase chain reaction,PCR (PCR) equimolecular biological means be master detection method cost height, technical difficulty greatly, need professional and instrument; With Chest X-rays or CT is that master's detection method can only be distinguished bacterial pneumonia and viral pneumonia; Can't make a definite diagnosis the streptococcus pneumonia pneumonia, some other method need be earned appearance (Invasive Sampling) to the patient, and the patient often need stand big painful.And, more than all methods all can not accomplish preventative diagnosis, what promptly all said methods detected is some human bodies or the focus characteristic that disease has taken place.Therefore it is extremely urgent to set up a kind of early detection diagnostic method simple, effective, quick, that do not have wound (Non-Invasive), preventative streptococcus pneumonia pneumonia.
Streptococcus pneumonia infecting in the human body lung can produce some specificity gaseous metabolism products; Especially working as streptococcus pneumonia adheres to the initial stage in lung; Because the change of living environment might make it produce the specificity volatile organic compound; Can be used as the chemical markers of the streptococcus pneumonia activity in lung before the pneumonia disease takes place, can diagnose and prevent the streptococcus pneumonia pneumonia through detecting these specific aerochemistry labels.These specificity gaseous metabolism products excrete through patient's expiratory air, therefore can the applying electronic nose etc. instrument detect to carry out the early stage and preventative diagnosis of streptococcus pneumonia pneumonia disease through expiratory air.But the method in the Electronic Nose electronic fingerprint data storehouse of associated pneumonia streptococcus pneumonia patient expiratory air does not have scientific and technical literature or patent report at present as yet.The present invention is directed to the patient's expiratory air and the healthy subjects expiratory air of pneumonia infection streptococcus initiation pneumonia; Utilize the effective detection technique of Electronic Nose simplicity of design; Detect the difference of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air rapidly and accurately, the method in the Electronic Nose electronic fingerprint data storehouse of a kind of streptococcus pneumonia pneumonia patient expiratory air is provided.
Summary of the invention
For addressing the above problem, the present invention provides a kind of method of electronic fingerprint data storehouse of pneumonia patient's expiratory air, and this method may further comprise the steps:
1. clean Tedlar gas production bag, remove airbag itself and external source volatile organic matter.(Shanghai chin or cheek Book of Music trade company limited) cleaned with high pure nitrogen (99.999%) with Tedlar gas production bag; Then the gas production bag is filled with high pure nitrogen and in 85 ℃ of condition held at least 8 hours; At last the gas production bag is cleaned with high pure nitrogen again; With vacuum pump the gas production bag is vacuumized, keep in Dark Place.
2. expiratory air sample collecting.The expiratory air gatherer process carried out in the non-meal time, avoided being captured in the streptococcus pneumonia pneumonia patient of edible stimulation oversaturation property smell food of meal time and the expiratory air of healthy subjects.Under the condition of patient and healthy subjects eupnea, at first that the air scavenge of lung about 1/3 is external, collect patient and the remaining expiratory air in deep of healthy subjects lung with the Tedlar gas production bag that cleans up then, until the gas production bag is full of.
3. set up the Electronic Nose electronic fingerprint data storehouse of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air.Utilize highly sensitive portable electric nose (PEN3; Germany AIRSENSE company) patient's expiratory air of check and analysis streptococcus pneumonia pneumonia and healthy subjects expiratory air; And through Electronic Nose on-board data conversion and storage system the volatile organic compound composition collection of illustrative plates (Breath VOC Profile) of all expiratory air samples is converted into digital signal and is stored in the Electronic Nose database; Set up the corresponding radar collection of illustrative plates of expiratory air component Electronic Nose gas sensor array, set up the Electronic Nose electronic fingerprint data storehouse of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air respectively.
It is simpler, quick, efficient, accurate, low-cost than the technical method of this programme temporarily still not have other scheme.This programme sampling makes the patient painless, and the instrumentation of use is simple, and volume is dexterous, be convenient to move carry, and it is lower to detect cost, has very high specificity and accuracy.
Description of drawings
According to accompanying drawing and embodiment the present invention is done further explain below.
The detection by electronic nose radar map of a typical healthy subjects expiratory air of Fig. 1, i.e. the Electronic Nose electronic fingerprint of this healthy subjects expiratory air.
The detection by electronic nose radar map of a typical pneumonia streptococcus pneumonia of Fig. 2 patient expiratory air, i.e. the Electronic Nose electronic fingerprint of this patient's expiratory air.
1-10 is a sensor.
Embodiment
A kind of method of setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air, this method may further comprise the steps:
1. clean Tedlar gas production bag: remove airbag itself and external source volatile organic matter; Tedlar gas production bag is cleaned with high pure nitrogen; Then the gas production bag is filled with high pure nitrogen and in 85 ℃ of condition held at least 8 hours; At last the gas production bag is cleaned with high pure nitrogen again, the gas production bag is vacuumized, keep in Dark Place with vacuum pump;
2. expiratory air sample collecting: the expiratory air gatherer process carried out in the non-meal time, avoided being captured in the streptococcus pneumonia pneumonia patient of edible stimulation oversaturation property smell food of meal time and the expiratory air of healthy subjects; Under the condition of patient and healthy subjects eupnea, at first that the air scavenge of lung about 1/3 is external, collect patient and the remaining expiratory air in deep of healthy subjects lung with the Tedlar gas production bag that cleans up then, until the gas production bag is full of;
3. set up the Electronic Nose electronic fingerprint data storehouse of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air: utilize highly sensitive portable electric nose check and analysis streptococcus pneumonia pneumonia patient's expiratory air and healthy subjects expiratory air; And through Electronic Nose on-board data conversion and storage system the volatile organic compound composition collection of illustrative plates (Breath VOC Profile) of all expiratory air samples is converted into digital signal and is stored in the Electronic Nose database; Set up the corresponding radar collection of illustrative plates of expiratory air component Electronic Nose gas sensor array, set up the Electronic Nose electronic fingerprint data storehouse of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air respectively.Said high pure nitrogen concentration is 99.999% to clean; Said highly sensitive portable electric nose is PEN3; The setup parameter of described detection by electronic nose is that the Electronic Nose probe scavenging period of healthy subjects expiratory air sample is 60s, and the Electronic Nose probe scavenging period of pneumonia patient's expiratory air sample is 180s; The Electronic Nose Measuring Time of two kinds of samples is all 50s.
Claims (6)
1. method of setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air, this method may further comprise the steps:
1. clean Tedlar gas production bag: remove airbag itself and external source volatile organic matter; Tedlar gas production bag is cleaned with high pure nitrogen; Then the gas production bag is filled with high pure nitrogen and in 85 ℃ of condition held at least 8 hours; At last the gas production bag is cleaned with high pure nitrogen again, the gas production bag is vacuumized, keep in Dark Place with vacuum pump;
2. expiratory air sample collecting: the expiratory air gatherer process carried out in the non-meal time, avoided being captured in the streptococcus pneumonia pneumonia patient of edible stimulation oversaturation property smell food of meal time and the expiratory air of healthy subjects; Under the condition of patient and healthy subjects eupnea, at first that the air scavenge of lung about 1/3 is external, collect patient and the remaining expiratory air in deep of healthy subjects lung with the Tedlar gas production bag that cleans up then, until the gas production bag is full of;
3. set up the Electronic Nose electronic fingerprint data storehouse of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air: utilize highly sensitive portable electric nose check and analysis streptococcus pneumonia pneumonia patient's expiratory air and healthy subjects expiratory air; And through Electronic Nose on-board data conversion and storage system the volatile organic compound composition collection of illustrative plates (Breath VOC Profile) of all expiratory air samples is converted into digital signal and is stored in the Electronic Nose database; Set up the corresponding radar collection of illustrative plates of expiratory air component Electronic Nose gas sensor array, set up the Electronic Nose electronic fingerprint data storehouse of patient's expiratory air of streptococcus pneumonia pneumonia and healthy subjects expiratory air respectively.
2. the method for setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air according to claim 1 is characterized in that: said high pure nitrogen concentration is 99.999% to clean.
3. the method for setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air according to claim 1 and 2 is characterized in that: at said highly sensitive portable electric nose is PEN3.
4. the method for setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air according to claim 1 and 2; It is characterized in that: the setup parameter of described detection by electronic nose is that the Electronic Nose probe scavenging period of healthy subjects expiratory air sample is 60s, and the Electronic Nose probe scavenging period of pneumonia patient's expiratory air sample is 180s; The Electronic Nose Measuring Time of two kinds of samples is all 50s.
5. the electronic fingerprint data storehouse that the method for setting up the electronic fingerprint data storehouse of pneumonia patient expiratory air according to claim 1 and 2 obtains.
6. the electronic fingerprint data storehouse of setting up pneumonia patient expiratory air according to claim 5 is characterized in that: the purposes that this database detects in the pneumonia that streptococcus pneumoniae infection causes.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318590A (en) * | 2017-12-25 | 2018-07-24 | 浙江大学 | Diagnostic system based on the pneumonia caused by the encountered pathogenic bacteria infection using exhaled gas marker spectrum |
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| WO1999065386A1 (en) * | 1998-06-15 | 1999-12-23 | The Trustees Of The University Of Pennsylvania | Diagnosing intrapulmonary infection and analyzing nasal sample |
| CN101334399A (en) * | 2008-07-15 | 2008-12-31 | 重庆大学 | Portable device for pulmonary cancer diagnosis |
| CN101620195A (en) * | 2009-08-12 | 2010-01-06 | 南京农业大学 | Method for detecting internal quality of jirou sweet persimmon by smell sensor |
| WO2010079490A1 (en) * | 2009-01-09 | 2010-07-15 | Technion Research And Development Foundation Ltd. | Volatile organic compounds as diagnostic markers in the breath for lung cancer |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999065386A1 (en) * | 1998-06-15 | 1999-12-23 | The Trustees Of The University Of Pennsylvania | Diagnosing intrapulmonary infection and analyzing nasal sample |
| CN101334399A (en) * | 2008-07-15 | 2008-12-31 | 重庆大学 | Portable device for pulmonary cancer diagnosis |
| WO2010079490A1 (en) * | 2009-01-09 | 2010-07-15 | Technion Research And Development Foundation Ltd. | Volatile organic compounds as diagnostic markers in the breath for lung cancer |
| CN101620195A (en) * | 2009-08-12 | 2010-01-06 | 南京农业大学 | Method for detecting internal quality of jirou sweet persimmon by smell sensor |
Non-Patent Citations (1)
| Title |
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| 陈星: "呼吸检测电子鼻及其在肺癌诊断应用中的研究", 《中国博士学位论文全文数据库（电子期刊）》, no. 9, 25 September 2008 (2008-09-25) * |
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| CN108318590A (en) * | 2017-12-25 | 2018-07-24 | 浙江大学 | Diagnostic system based on the pneumonia caused by the encountered pathogenic bacteria infection using exhaled gas marker spectrum |
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Application publication date: 20120425 |