CN102426212B - Enhanced fluorescence thin layer identification method for Cyperus Rotundus L - Google Patents
Enhanced fluorescence thin layer identification method for Cyperus Rotundus L Download PDFInfo
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- CN102426212B CN102426212B CN201110246268.XA CN201110246268A CN102426212B CN 102426212 B CN102426212 B CN 102426212B CN 201110246268 A CN201110246268 A CN 201110246268A CN 102426212 B CN102426212 B CN 102426212B
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Abstract
The invention discloses an enhanced fluorescence thin layer identification method for Chinese herbal medicines. According to the invention, an enhanced fluorescer is added on the base of ordinary thin layer identification, ideal results are obtained in identification of a plurality of Chinese herbal medicines which have no detection information, detection approaches of thin layer identification are greatly broadened, and a thin layer identification revision rate of prescribed Chinese herbal preparations can be effectively improved.
Description
patented claim of the present invention is divisional application, and original bill information is as follows:
application number: 2010105281179
the applying date: on November 2nd, 2010
invention and created name: a kind of increasing fluoroscopic examination thin-layer identification method of Chinese crude drug.
Technical field
The present invention relates to a kind of thin-layer identification method of Chinese crude drug, particularly, relate to the increasing fluorescence thin layer discrimination method of a kind of rhizoma cyperi.
Background technology
At present in all kinds of quality standard of Chinese medicament preparation of country, it is low that the TLC distinguish of its medicinal material revises and enlarges rate, is on average about 30% of prescription Chinese crude drug total amount, cannot the conduct monitoring at all levels quality of the pharmaceutical preparations.Especially extract preparation, the not attached existence of microscopic features of medicinal material, microscopical characters is felt simply helpless to it, and TLC distinguish is found less than characteristic Detection Information again, just the uncontrollable quality of the pharmaceutical preparations.Cause some illegal operators to use inferior materials and turn out substandard goods in the production run of medicine, occur with bad Dai You, the situation of passing a fake product off as a genuine one, the lighter affects the state of an illness of patient adversely, and severe one directly endangers the health of patient.
Summary of the invention
Detect approach for widening TLC distinguish, the TLC distinguish improved in Traditional Chinese medicine historical preparation revises and enlarges rate, and inventor has carried out the research of increasing fluorescence to the multiple Chinese crude drug without Detection Information, and obtains desirable result.
The invention provides a kind of increasing fluoroscopic examination thin-layer identification method of Chinese crude drug, the method comprises following steps:
A, get test sample medicinal material and control medicinal material powder 0.01-0.5 gram respectively, add methyl alcohol 3-10 milliliter, ultrasonic process 10-15 minute, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 2-4 microlitre of reference substance solution, put respectively in same GF
254on thin layer plate, launch with developping agent, take out, dry;
C, thin layer plate spray with fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of test sample chromatogram aobvious same color on the position corresponding to reference substance chromatogram.
Preferably, the Chinese crude drug of thin-layer identification method kind of the present invention is preferably the one in the dried venom of toads, the bighead atractylodes rhizome, the capsule of weeping forsythia, calculus bovis factitius, the root of three-nerved spicebush, oriental wormwood, moutan bark or rhizoma cyperi, increases fluorescer and is preferably 10% ethanol solution of sulfuric acid or 1% aluminium choride ethanolic solution.
In theory, originally without the Chinese crude drug of any Detection Information, TLC distinguish can be carried out by the method for increasing fluorescence of the present invention, inventor's particularly preferably increasing fluoroscopic examination thin-layer identification method of several medicinal material, as follows respectively:
1, the increasing fluoroscopic examination thin-layer identification method of the dried venom of toads is preferably:
A, get dried venom of toads medicinal material and 0.05 gram, dried venom of toads control medicinal material powder respectively, add methyl alcohol 3 milliliters, ultrasonic process 15 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 2 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 4:6:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of dried venom of toads test sample chromatogram aobvious same color on the position corresponding to dried venom of toads reference substance chromatogram.
2, the increasing fluoroscopic examination thin-layer identification method of the bighead atractylodes rhizome is preferably:
A, get Rhizoma Atractylodis Macrocephalae and 0.5 gram, bighead atractylodes rhizome control medicinal material powder respectively, add methyl alcohol 5 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 8:2:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of bighead atractylodes rhizome test sample chromatogram aobvious same color on the position corresponding to bighead atractylodes rhizome reference substance chromatogram.
3, the increasing fluoroscopic examination thin-layer identification method of the capsule of weeping forsythia is preferably:
A, get capsule of weeping forsythia medicinal material and 0.4 gram, capsule of weeping forsythia control medicinal material powder respectively, add methyl alcohol 5 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 10:2:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of capsule of weeping forsythia test sample chromatogram aobvious same color on the position corresponding to capsule of weeping forsythia reference substance chromatogram.
4, the increasing fluoroscopic examination thin-layer identification method of calculus bovis factitius is preferably:
A, get calculus bovis factitius medicinal material and 0.15 gram, calculus bovis factitius control medicinal material powder respectively, add methyl alcohol 6 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 2 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-acetic acid-methyl alcohol of 10:25:2:3 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of calculus bovis factitius test sample chromatogram aobvious same color on the position corresponding to calculus bovis factitius reference substance chromatogram.
5, the increasing fluoroscopic examination thin-layer identification method of the root of three-nerved spicebush is preferably:
A, get root of three-nerved spicebush medicinal material and 0.3 gram, root of three-nerved spicebush control medicinal material powder respectively, add methyl alcohol 4 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 8:2:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of root of three-nerved spicebush test sample chromatogram aobvious same color on the position corresponding to root of three-nerved spicebush reference substance chromatogram.
6, the increasing fluoroscopic examination thin-layer identification method of oriental wormwood is preferably:
A, get oriental wormwood medicinal material and 0.4 gram, oriental wormwood control medicinal material powder respectively, add methyl alcohol 8 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate of 7:3 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of oriental wormwood test sample chromatogram aobvious same color on the position corresponding to oriental wormwood reference substance chromatogram.
7, the increasing fluoroscopic examination thin-layer identification method of moutan bark is preferably:
A, get moutan bark medicinal material and 0.01 gram, moutan bark control medicinal material powder respectively, add methyl alcohol 10 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 3 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-acetone-formic acid of 8:2:1:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 1% aluminium choride ethanolic solution as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of moutan bark test sample chromatogram aobvious same color on the position corresponding to moutan bark reference substance chromatogram.
8, the increasing fluoroscopic examination thin-layer identification method of rhizoma cyperi is preferably:
A, get rhizoma cyperi medicinal material and 0.5 gram, rhizoma cyperi control medicinal material powder respectively, add methyl alcohol 4 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 10:2:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of rhizoma cyperi test sample chromatogram aobvious same color on the position corresponding to rhizoma cyperi reference substance chromatogram.
The thin-layer chromatogram comparative analysis before and after fluorescence is increased from 8 kinds of medicinal materials, before increasing fluorescence, no matter under 254nm or 365nm, all present without Detection Information clearly, cannot judge result, but after increasing fluorescence, the amplitude of containing much information increases, sensitivity improves, as the root of three-nerved spicebush presents 8 ~ 9 indigo plants, green fluorescence spot; The bighead atractylodes rhizome presents 5 ~ 6 indigo plants, green fluorescence spot; The capsule of weeping forsythia presents 3 Huangs and bright blue fluorescence spot etc., and clear spot, intensity is very high, can be used for qualitative even quantitative examination, each thin-layer chromatogram of detailed in Example testing result.
In a word, the present invention increases fluoroscopic examination thin-layer identification method and has sensitive, quick, efficient feature, solve not only without uv absorption, but also the discriminating problem of non-blooming medicinal material, enable the discriminating high sensitivity of these medicinal materials, carry out exactly, revise and enlarge rate to the TLC distinguish improving Chinese crude drug and Traditional Chinese medicine historical preparation, one has use value to invent very much beyond doubt.
Accompanying drawing explanation
Fig. 1 dried venom of toads TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, and latter three is control medicinal material.
Fig. 2 bighead atractylodes rhizome TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, and latter three is control medicinal material.
Fig. 3 capsule of weeping forsythia TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, and latter three is control medicinal material.
Fig. 4 calculus bovis factitius TLC distinguish collection of illustrative plates; A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence; from left to right first three is sample to spot, and latter two is control medicinal material.
Fig. 5 root of three-nerved spicebush TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, and latter two is control medicinal material.
Fig. 6 oriental wormwood TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, and latter two is control medicinal material.
Fig. 7 moutan bark TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, and B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, and C inspects photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, and latter three is control medicinal material.
Fig. 8 rhizoma cyperi TLC distinguish collection of illustrative plates, A is for inspecting photo under 254nm uviol lamp before increasing fluorescence, B is for inspecting photo under 365nm uviol lamp before increasing fluorescence, C is for inspecting photo under 365nm uviol lamp after increasing fluorescence, from left to right first three is sample to spot, following three is control medicinal material, and latter two is Rhizoma Cyperi (vinegar processed).
Embodiment
Following embodiment is for illustrating the embodiment of the inventive method, but it can not form any restriction to scope of the present invention, for the technique effect of proved inventive method, each embodiment is all after drying with developping agent expansion, before spray increases fluorescence, inspect under 254nm and 365nm uviol lamp and take pictures, compare to make to increase chromatogram after fluorescence.
Embodiment 1
The increasing fluoroscopic examination TLC distinguish of the dried venom of toads:
A, get dried venom of toads medicinal material and 0.05 gram, dried venom of toads control medicinal material powder respectively, add methyl alcohol 3 milliliters, ultrasonic process 15 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 2 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 4:6:0.2 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 1, the fluorescence spot of dried venom of toads test sample chromatogram aobvious 4 same colors on the position corresponding to dried venom of toads reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 2
The increasing fluoroscopic examination TLC distinguish of the bighead atractylodes rhizome:
A, get Rhizoma Atractylodis Macrocephalae and 0.5 gram, bighead atractylodes rhizome control medicinal material powder respectively, add methyl alcohol 5 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 8:2:0.2 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 2, the fluorescence spot of bighead atractylodes rhizome test sample chromatogram aobvious multiple same color on the position corresponding to dried venom of toads reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 3
The increasing fluoroscopic examination TLC distinguish of the capsule of weeping forsythia:
A, get capsule of weeping forsythia medicinal material and 0.4 gram, capsule of weeping forsythia contrast medicine inborn ability end respectively, add methyl alcohol 5 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 10:2:0.2 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 3, the fluorescence spot of capsule of weeping forsythia test sample chromatogram aobvious 3 same colors on the position corresponding to capsule of weeping forsythia reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 4
The increasing fluoroscopic examination TLC distinguish of calculus bovis factitius:
A, get calculus bovis factitius medicinal material and 0.15 gram, calculus bovis factitius control medicinal material powder respectively, add methyl alcohol 6 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 2 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-acetic acid-methyl alcohol of 10:25:2:3 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 4; the fluorescence spot of calculus bovis factitius test sample chromatogram aobvious 4 same colors on the position corresponding to calculus bovis factitius reference substance chromatogram after visible increasing fluorescence; and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 5
The increasing fluoroscopic examination TLC distinguish of the root of three-nerved spicebush:
A, get root of three-nerved spicebush medicinal material and 0.3 gram, root of three-nerved spicebush control medicinal material powder respectively, add methyl alcohol 4 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 8:2:0.2 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 5, the fluorescence spot of root of three-nerved spicebush test sample chromatogram aobvious multiple same color on the position corresponding to root of three-nerved spicebush reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 6
The increasing fluoroscopic examination TLC distinguish of oriental wormwood:
A, get oriental wormwood medicinal material and 0.4 gram, oriental wormwood control medicinal material powder respectively, add methyl alcohol 8 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate of 7:3 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 6, the fluorescence spot of oriental wormwood test sample chromatogram aobvious multiple same color on the position corresponding to oriental wormwood reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 7
The increasing fluoroscopic examination TLC distinguish of moutan bark:
A, get moutan bark medicinal material and 0.01 gram, moutan bark control medicinal material powder respectively, add methyl alcohol 10 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 3 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-acetone-formic acid of 8:2:1:0.2 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 1% aluminium choride ethanolic solution as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 7, the fluorescence spot of moutan bark test sample chromatogram aobvious 1 same color on the position corresponding to moutan bark reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Embodiment 8
The increasing fluoroscopic examination TLC distinguish of rhizoma cyperi:
A, get rhizoma cyperi medicinal material and 0.5 gram, rhizoma cyperi control medicinal material powder respectively, add methyl alcohol 4 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 10:2:0.2 launches for developping agent with volume ratio, take out, dry, inspect under 254nm and 365nm uviol lamp respectively and take pictures;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect and take pictures;
The results are shown in Figure 8, the fluorescence spot of rhizoma cyperi test sample chromatogram aobvious multiple same color on the position corresponding to rhizoma cyperi reference substance chromatogram after visible increasing fluorescence, and do not increase in the thin-layer chromatography of fluorescence, under 254nm and 365nm uviol lamp, all do not show obvious spot.
Claims (1)
1. an increasing fluorescence thin layer discrimination method for rhizoma cyperi, is characterized in that the method comprises following steps:
A, get rhizoma cyperi medicinal material and 0.5 gram, rhizoma cyperi control medicinal material powder respectively, add methyl alcohol 4 milliliters, ultrasonic process 10 minutes, get supernatant as need testing solution and reference substance solution;
B, absorption need testing solution and each 4 microlitres of reference substance solution, put respectively in same GF
254on thin layer plate, be that the cyclohexane-ethyl acetate-formic acid of 10:2:0.2 launches for developping agent with volume ratio, take out, dry;
C, thin layer plate spray using 10% ethanol solution of sulfuric acid as fluorescence-enhancing agent, 105 DEG C to be heated to spot development clear;
D, put 365nm uviol lamp under inspect, the fluorescence spot of rhizoma cyperi test sample chromatogram aobvious same color on the position corresponding to rhizoma cyperi reference substance chromatogram.
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