CN102415922B - Controllable vascularized artificial bone graft - Google Patents
Controllable vascularized artificial bone graft Download PDFInfo
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- CN102415922B CN102415922B CN201110262436.4A CN201110262436A CN102415922B CN 102415922 B CN102415922 B CN 102415922B CN 201110262436 A CN201110262436 A CN 201110262436A CN 102415922 B CN102415922 B CN 102415922B
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Abstract
The invention discloses a controllable vascularized artificial bone graft in which a soft tissue window, a soft tissue window anchor bearing and a cell-stent complex match with each other to be integrated, wherein the cell-stent complex is made of porous medical alloy compounded with osteoblasts; and the soft tissue window and the soft tissue window anchor bearing are both made of medical absorbable materials, and the contact area of the artificial bone graft and a host soft tissue is controlled through adjusting the aperture of the soft tissue window so as to further realize the controllability of graft vascularization. The controllable vascularized artificial bone graft can realize the controllable vascularization after grafting, provides a good nutrition supply and metabolism environment for grafting cells, and simultaneously avoids local excessive vascular fibration of the graft, promotes osteogenic ability after artificial bone grafting, and effectively improves the clinical effect.
Description
Technical field
The invention belongs to biomedical engineering field, be specifically related to a kind of " soft tissue window " formula controllable vascularization artificial bone graft, the degree of vascularization while being mainly used in controlling the artificial bonegraft repairing bone defect, guarantee the artificial bonegraft activity, promotes bone defect repair effect.
Background technology
Bone is damaged is the common disease in Orthopedic Clinical, mainly adopts clinically at present bone grafting treatment bone damaged, promotes union of fracture.Adopt the autologous bone transplanting repairing bone defect, be still so far " goldstandard " that bone is transplanted.But the bone amount of autologous bone is limited, and get osseous surgery increased the patient wound and painful, extended operating time, exist for district infect, the complication such as hemorrhage, pain.Though the bone graft substitutes such as homogeneous allogenic bone, xenogenesis bone can be avoided second operation, the bone repairing effect is not good enough, and has the risk of immunologic rejection and communicate illness.
The clinical treatment that bone is damaged that develops into of the proposition of organizational project concept and bone tissue engineer technology provides a kind of new selection.Utilizing tissue engineering technique to build the basic skills of osseous tissue, is that osteogenic seed cell and various timbering material are formed to the artificial bonegraft repairing bone defect.Its bone formation performance and autologous bone approach, and have bone conductibility, osteoinductive and osteogenesis concurrently, are not subject to bone quantitative limitation ，Wu Gong district's complication and immunological rejection and pathophorous danger, are a kind of ideal bone grafts simultaneously.Yet, build effect although can reach satisfied artificial bone in laboratory, but can't obtain in time sufficient nutritional support after surviving in vitro good artificial bonegraft and implanting, the cell mortality, be difficult to bring into play osteogenic activity, therefore in clinical practice, be not widely used yet up to now, its main cause is:
(1) bone graft vascularization deficiency: good blood vessel is grown into and can be guaranteed the nutrition supply that transplanted cells is enough, and timely scavenger cell metabolite, for cell provides good metabolism environment.If the bone substitute vascularization degree in vivo deficiency of implanting, can't provide sufficient blood confession for internal stent, can cause for want of nutrient and dead rapidly of bone seeding cell in support, seriously reduced osteoinductive and the osteogenesis of artificial bone.
(2) bone graft local excessive vascularization: there are some researches show the inflammation and the fibrosis problem that exist excessive vascularization to cause in graft and part, host tissue interface (especially soft tissue interface) after graft implants., form fibrosis in graft surface and intercept far away faster than the area of new bone speed of growing into due to formed FCTG speed, cause the new vessels artificial bonegraft inside that is difficult to grow into, transplanted cells, because of malnutrition death, makes to repair fall through.
Therefore, how to realize artificial bone substitute in object transplant after the controllability of vascularization, can either guarantee that graft is whole fast, fully when vascularization, can avoid the local excessive vascular fibrosis again, become the problem that solution is needed in its clinical practice badly.
Summary of the invention
The defect or the deficiency that for above-mentioned prior art, exist, the object of the invention is to, build a kind of controllable vascularization artificial bone graft, controlled vascularization after transplanting with realization, effectively improve clinical efficacy, for transplanted cells provides good nutrition supply and metabolism environment, avoid graft local excessive vascular fibrosis simultaneously, osteogenic activity after the promotion artificial bone graft, promote the extensive clinical practice of tissue engineered artificial bone graft.
For realizing above-mentioned task, the present invention takes following technical solution:
A kind of controllable vascularization artificial bone graft, it is characterized in that, this controllable vascularization artificial bone graft is cooperatively interacted and is become one by soft tissue window, soft tissue window anchoring part and cell-scaffold construct, wherein, the material of soft tissue window and soft tissue window anchoring part is the Medical absorbable material, and the material of cell-scaffold construct is medical alloy;
The shape of described soft tissue window is thin rounded flakes, and thin rounded flakes central authorities are with the adjustable through hole of circle; The shape of described soft tissue window anchoring part is annulus, and soft tissue window is embedded on the soft tissue window anchoring part; Described cell-scaffold construct is the Round Porous column, and equally distributed osteoblast is arranged in cell-scaffold construct, and cell density is 4 * 10
4/ mm
2, cell-scaffold construct and soft tissue window anchoring part connect together, and form gap between cell-scaffold construct and soft tissue window.
Controllable vascularization artificial bone graft of the present invention, its innovative point is:
But 1 directly transplanting defect to the marrow carries out defect repair, by adjusting the size in soft tissue window aperture, control the contact area of artificial bonegraft and host's soft tissue, guarantee that appropriate soft tissue grows into, and then realize the controllability of graft vascularization, be beneficial to and realize that graft is whole fast, fully vascularization.
2, cell-scaffold construct large tracts of land contact soft tissue has been avoided in the setting of soft tissue window, controls the vascularization process of rack surface, thereby prevents graft local excessive vascular fibrosis, the osteogenic activity after guarantee and promotion artificial bone graft.
3, the support of cell-scaffold construct is titanium alloy (material is Ti-6Al-4V), adopt the manufacture of electron-beam melting shaping (EBM) technology, there is controlled internal crosslinking loose structure and good biological activity, be beneficial to new vessels grow into and support in the formation of new vessels net; Adopt filling type bioreactor and dynamic cellular inoculation-culture technique to be combined into osteocyte, cell density reaches 4 * 10
4/ mm
2, cell is uniformly distributed in support, is beneficial to area of new bone and forms and the bone defect repair.
4, soft tissue window anchoring part and soft tissue window are the Medical absorbable material manufacture, have no side effect in vivo and good biocompatibility, but after implanting, slow degradation absorb, and avoid the risk of second operation.
The accompanying drawing explanation
Fig. 1 is controllable vascularization artificial bone graft structural representation of the present invention;
Fig. 2 is controllable vascularization artificial bone graft cross-sectional view of the present invention;
Fig. 3 is controllable vascularization artificial bone graft preparation process schematic diagram of the present invention; Wherein, (a) mean soft tissue window is assemblied on the soft tissue window anchoring part, (b) mean the soft tissue window anchoring part is press-fit on cell-scaffold construct, (c) mean the cell-scaffold construct implantable bone is damaged in.
Below in conjunction with accompanying drawing, the present invention is described in further details.
The specific embodiment
Shown in accompanying drawing 1-3, the present embodiment provides a kind of controllable vascularization artificial bone graft, by soft tissue window 1, soft tissue window anchoring part 2 and cell-scaffold construct 3, is cooperatively interacted and is become one.
The material of soft tissue window 1 is the Medical absorbable material, the present embodiment is selected polycaprolactone, the vivo degradation cycle is 1-2, the employing rapid shaping technique is manufactured, and making diameter is the thin rounded flakes that D1, thickness are H1, and central authorities are with circular adjustability through hole D2, the aperture adjustable extent of through hole D2 is 2mm-10mm, by adjusting the size in soft tissue window 1 aperture, control artificial bonegraft and host's soft tissue contact area, and then realize the controllability of graft vascularization.
The material of soft tissue window anchoring part 2 is the Medical absorbable material, and the present embodiment is also selected polycaprolactone, adopts the rapid shaping technique manufacture, and making diameter is D3, and thickness is D4, is highly the annulus of H2, for (inlaying) soft tissue window 1 is installed.
The material of cell-scaffold construct 3 is medical alloy, the present embodiment is selected titanium alloy (material is Ti-6Al-4V), adopt the manufacture of electron-beam melting shaping (EBM) technology, make the Round Porous column, connection rate 100% between the space network that pore morphology is simulation C60 atomic space formation, aperture 1000 μ m, porosity 70%, hole.For H3, diameter, be highly D5.Adopt filling type bioreactor and dynamically inoculate-culture technique of cell composite reactive osteoblast in support, reach 4 * 10
4the cell load-carry duty of cell/surface area.
Adopt filling type bioreactor and dynamic cellular inoculation-culture technique, on cell-scaffold construct 3, be combined into osteocyte, cell density reaches 4 * 10
4/ mm
2, cell is uniformly distributed in cell-scaffold construct 3.Above-mentioned soft tissue window anchoring part 2 is press-fit on cell-scaffold construct 3, soft tissue window anchoring part 2 is H4 with the height of cell-scaffold construct 3 intersection, make to form gap between cell-scaffold construct 3 and soft tissue window 1, grow into for new vessels network and soft tissue, for transplanted cells growth and freshman bone tissue form, provide good nutrition supply and metabolism environment.
Operation of the present invention is as shown in Figure 3: at first according to the virtual condition of bone damaged 4, select to be applicable to the soft tissue window 1 of bore dia, and it is embedded in to (a step in Fig. 3) on soft tissue window anchoring part 2, the soft tissue window anchoring part 2 of organizing window 1 will be installed again and be press-fit in cell-scaffold construct 3 (the b step in Fig. 3) top, after having assembled, the controllable vascularization artificial bone graft of making is positioned over to damaged 4 places of bone (the c step in Fig. 3) and is repaired.
It should be noted that, in above-described embodiment, cell-scaffold construct 3 also can be selected any medical material, is not limited to titanium alloy.Soft tissue window 1 and soft tissue window anchoring part 2 also can select any Medical absorbable material, also be not limited to adopt polycaprolactone, every employing structure of the present invention, all should belong to protection scope of the present invention.
Claims (6)
1. a controllable vascularization artificial bone graft, it is characterized in that, this controllable vascularization artificial bone graft is cooperatively interacted and is become one by soft tissue window (1), soft tissue window anchoring part (2) and cell-scaffold construct (3), wherein, the material of soft tissue window (1) and soft tissue window anchoring part (2) is the Medical absorbable material, and the material of cell-scaffold construct (3) is medical alloy;
The shape of described soft tissue window (1) is thin rounded flakes, and thin rounded flakes central authorities are with the adjustable through hole of circle; The shape of described soft tissue window anchoring part (2) is annulus, and soft tissue window (1) is embedded on soft tissue window anchoring part (2); Described cell-scaffold construct (3) is the Round Porous column, and cell-scaffold construct has equally distributed osteoblast in (3), and cell density is 4 * 10
4/ mm
2, cell-scaffold construct (3) and soft tissue window anchoring part (2) connect together, and form gap between cell-scaffold construct (3) and soft tissue window (1).
2. controllable vascularization artificial bone graft as claimed in claim 1, is characterized in that, described medical alloy is titanium alloy.
3. controllable vascularization artificial bone graft as claimed in claim 2, is characterized in that, the material of described titanium alloy is Ti-6Al-4V.
4. controllable vascularization artificial bone graft as claimed in claim 1, is characterized in that, described Medical absorbable material is polycaprolactone.
5. controllable vascularization artificial bone graft as claimed in claim 1, is characterized in that, the diameter range of the adjustable through hole of described circle is 2mm-10mm.
6. controllable vascularization artificial bone graft as claimed in claim 1, it is characterized in that connection rate 100% between the space network that the pore morphology of described cell-scaffold construct (3) is simulation C60 atomic space formation, aperture 1000 μ m, porosity 70%, hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110262436.4A CN102415922B (en) | 2011-09-06 | 2011-09-06 | Controllable vascularized artificial bone graft |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110262436.4A CN102415922B (en) | 2011-09-06 | 2011-09-06 | Controllable vascularized artificial bone graft |
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| CN102415922A CN102415922A (en) | 2012-04-18 |
| CN102415922B true CN102415922B (en) | 2014-01-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CA2935545A1 (en) * | 2013-12-30 | 2015-07-09 | New York Stem Cell Foundation, Inc. | Tissue grafts and methods of making and using the same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304711A (en) * | 2000-12-15 | 2001-07-25 | 华西医科大学附属第一医院 | Bio-derivative tissue engineering bone and its preparing process |
| CN1972644A (en) * | 2003-11-21 | 2007-05-30 | 骨棚国际私人有限公司 | Bioabsorbable plug implants and method for bone tissue regeneration |
| CN202223387U (en) * | 2011-09-06 | 2012-05-23 | 中国人民解放军第四军医大学 | Controllable vascularization artificial bone graft |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080077251A1 (en) * | 1999-06-07 | 2008-03-27 | Chen Silvia S | Cleaning and devitalization of cartilage |
| US20080154379A1 (en) * | 2006-12-22 | 2008-06-26 | Musculoskeletal Transplant Foundation | Interbody fusion hybrid graft |
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2011
- 2011-09-06 CN CN201110262436.4A patent/CN102415922B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304711A (en) * | 2000-12-15 | 2001-07-25 | 华西医科大学附属第一医院 | Bio-derivative tissue engineering bone and its preparing process |
| CN1972644A (en) * | 2003-11-21 | 2007-05-30 | 骨棚国际私人有限公司 | Bioabsorbable plug implants and method for bone tissue regeneration |
| CN202223387U (en) * | 2011-09-06 | 2012-05-23 | 中国人民解放军第四军医大学 | Controllable vascularization artificial bone graft |
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| CN102415922A (en) | 2012-04-18 |
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