CN102415922A - Controllable vascularized artificial bone graft - Google Patents
Controllable vascularized artificial bone graft Download PDFInfo
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- CN102415922A CN102415922A CN2011102624364A CN201110262436A CN102415922A CN 102415922 A CN102415922 A CN 102415922A CN 2011102624364 A CN2011102624364 A CN 2011102624364A CN 201110262436 A CN201110262436 A CN 201110262436A CN 102415922 A CN102415922 A CN 102415922A
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- soft tissue
- cell
- tissue window
- artificial
- bonegraft
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Abstract
The invention discloses a controllable vascularized artificial bone graft in which a soft tissue window, a soft tissue window anchor bearing and a cell-stent complex match with each other to be integrated, wherein the cell-stent complex is made of porous medical alloy compounded with osteoblasts; and the soft tissue window and the soft tissue window anchor bearing are both made of medical absorbable materials, and the contact area of the artificial bone graft and a host soft tissue is controlled through adjusting the aperture of the soft tissue window so as to further realize the controllability of graft vascularization. The controllable vascularized artificial bone graft can realize the controllable vascularization after grafting, provides a good nutrition supply and metabolism environment for grafting cells, and simultaneously avoids local excessive vascular fibration of the graft, promotes osteogenic ability after artificial bone grafting, and effectively improves the clinical effect.
Description
Technical field
The invention belongs to biomedical engineering field; Be specifically related to the controlled vascularization artificial bonegraft of a kind of " soft tissue window " formula; The degree of vascularization when being mainly used in control artificial bonegraft repairing bone defect guarantees that artificial bonegraft is active, promotes bone defect repair effect.
Background technology
Bone is damaged to be the common disease of orthopaedics in clinical, mainly adopts bone grafting treatment bone damaged at present clinically, promotes union of fracture.Adopt the autologous bone transplanting repairing bone defect, be still " goldstandard " that bone is transplanted so far.But, limited from the bone amount of body bone, and get wound that osseous surgery increased the patient with painful, prolonged operating time, have complication such as supplying the district infects, hemorrhage, pain.Though bone graft substitutes such as homogeneous allogenic bone, xenogenesis bone can be avoided second operation, the bone repairing effect is not good enough, and has the risk of immunologic rejection and communicate illness.
The damaged clinical treatment of bone that develops into of the proposition of organizational project notion and bone tissue engineer technology provides a kind of new selection.Utilizing tissue engineering technique to make up the basic skills of osseous tissue, is that skeletonization property seed cell and various timbering material are formed the artificial bonegraft repairing bone defect.Its bone formation performance has bone conductibility, osteoinductive and osteogenesis property concurrently with approaching from the body bone, does not receive the bone quantitative limitation simultaneously, does not have the district's of confession complication and immunological rejection and risk of disease transmission, is a kind of very ideal bone graft.Yet; Make up effect although in laboratory, can reach satisfied artificial bone; But after external one-tenth good artificial bonegraft alive implants, can't in time obtain the sufficient nutrition support, the cell mortality is difficult to bring into play osteogenic activity; Therefore in clinical practice, do not obtain widespread usage up to now yet, its main cause is:
(1) the bone graft vascularization is not enough: good blood vessel is grown into and can be guaranteed the nutrition supply that transplanted cells is enough, and timely scavenger cell metabolite is for cell provides good metabolism environment.If the bone substitute vascularization degree of implanting in vivo is not enough, can't supply for internal stent provide competent blood, can cause the bone seeding cell nutrient and dead rapidly for want of in the support, seriously reduced the osteoinductive and the osteogenesis property of artificial bone.
(2) bone graft local excessive vascularization: there are some researches show graft inflammation and the fibrosis problem that the back exists excessive vascularization to cause in graft and part, host tissue interface (especially soft tissue interface) that implant.Because formed FCTG speed far away faster than the area of new bone speed of growing into, forms fibrosis in graft surface and intercepts, cause the new vessels artificial bonegraft inside that is difficult to grow into, transplanted cells is dead because of malnutrition, makes the reparation fall through.
Therefore, how to realize that artificial bone substitutes the controllability of transplanting the back vascularization in the object, can either guarantee that graft is whole fast, fully vascularization the time, can avoid the local excessive vascular fibrosis again, become the problem that solution is needed in its clinical practice badly.
Summary of the invention
Defective or deficiency to above-mentioned prior art exists the objective of the invention is to, and make up a kind of controlled vascularization artificial bonegraft; With the controlled vascularization after the realization transplanting; Effectively improve clinical efficacy,, avoid graft local excessive vascular fibrosis simultaneously for transplanted cells provides good nutrition supply and metabolism environment; Osteogenic activity behind the promotion artificial bone graft promotes the extensive clinical practice of tissue engineered artificial bone graft.
For realizing above-mentioned task, the present invention takes following technical solution:
A kind of controlled vascularization artificial bonegraft; It is characterized in that; This controlled vascularization artificial bonegraft is cooperatively interacted by soft tissue window, soft tissue window anchoring part and cell-scaffold complex and becomes one; Wherein, the material of soft tissue window and soft tissue window anchoring part is medical absorbable material, and the material of cell-scaffold complex is a medical alloy;
The shape of described soft tissue window is a thin rounded flakes, and thin rounded flakes central authorities have circular adjustable through hole; The shape of described soft tissue window anchoring part is an annulus, and soft tissue window is embedded on the soft tissue window anchoring part; Described cell-scaffold complex is the porous circular cylinder shape, and it is 4 * 10 that equally distributed osteoblast, cell density are arranged in the cell-scaffold complex
4/ mm
2, cell-scaffold complex and soft tissue window anchoring part connect together, and form the gap between cell-scaffold complex and the soft tissue window.
Controlled vascularization artificial bonegraft of the present invention, its innovative point is:
But 1 directly transplanting defect to the marrow carries out defect repair; Size through adjustment soft tissue window aperture; The contact area of control artificial bonegraft and host's soft tissue; Guarantee that an amount of soft tissue grows into, and then realize the controllability of graft vascularization, be beneficial to and realize that graft is whole fast, fully vascularization.
2, cell-scaffold complex large tracts of land contact soft tissue has been avoided in the setting of soft tissue window, the vascularization process of control rack surface, thus prevent graft local excessive vascular fibrosis, the osteogenic activity behind guarantee and the promotion artificial bone graft.
3, the support of cell-scaffold complex is titanium alloy (material is Ti-6Al-4V); Adopt electron-beam melting (EBM) technology that is shaped to make; Have controlled internal crosslinking loose structure and good biological activity, be beneficial to new vessels grow into and support in the formation of new vessels net; Adopt filling type bioreactor and dynamic cellular inoculation-culture technique to be combined into osteocyte, cell density reaches 4 * 10
4/ mm
2, cell is uniform distribution in support, is beneficial to area of new bone and forms and the bone defect repair.
4, soft tissue window anchoring part and soft tissue window are medical absorbable material manufacturing, have no side effect in vivo and good biocompatibility, and slow degraded and absorbed after implanting is avoided the risk of second operation.
Description of drawings
Fig. 1 is a controlled vascularization artificial bonegraft structural representation of the present invention;
Fig. 2 is a controlled vascularization artificial bonegraft cross-sectional view of the present invention;
Fig. 3 is that controlled vascularization artificial bonegraft of the present invention prepares the process sketch map; Wherein, (a) expression soft tissue window is assemblied on the soft tissue window anchoring part, (b) expression soft tissue window anchoring part is press-fit on the cell-scaffold complex, (c) expression with cell-scaffold complex implantable bone damaged in.
Below in conjunction with accompanying drawing the present invention is explained further details.
The specific embodiment
Shown in accompanying drawing 1-3, present embodiment provides a kind of controlled vascularization artificial bonegraft, and being cooperatively interacted by soft tissue window 1, soft tissue window anchoring part 2 and cell-scaffold complex 3 becomes one.
The material of soft tissue window 1 is medical absorbable material, and present embodiment is selected polycaprolactone for use, and the vivo degradation cycle is 1-2; The employing rapid shaping technique is made, and processing diameter is that D1, thickness are the thin rounded flakes of H1, and central authorities have circular adjustability through hole D2; The aperture adjustable extent of through hole D2 is 2mm-10mm; Through the size in adjustment soft tissue window 1 aperture, control artificial bonegraft and host's soft tissue contact area, and then the controllability of realization graft vascularization.
The material of soft tissue window anchoring part 2 is medical absorbable material, and present embodiment is also selected polycaprolactone for use, adopts the rapid shaping technique manufacturing, and processing diameter is D3, and thickness is D4, highly is the annulus of H2, supplies to install (inlaying) soft tissue window 1 and uses.
The material of cell-scaffold complex 3 is a medical alloy; Present embodiment is selected titanium alloy (material is Ti-6Al-4V) for use; Adopt electron-beam melting (EBM) technology that is shaped to make; Process the porous circular cylinder shape, pore morphology is connection rate 100% between the space network of simulation C60 former subspace formation, aperture 1000 μ m, porosity 70%, hole.Be D5 highly for H3, diameter.Adopt filling type bioreactor and cell dynamically to inoculate-culture technique composite reactive osteoblast in support, reach 4 * 10
4The cell load-carry duty of cell/surface area.
Adopt filling type bioreactor and dynamic cellular inoculation-culture technique, on cell-scaffold complex 3, be combined into osteocyte, cell density reaches 4 * 10
4/ mm
2, cell is uniform distribution in cell-scaffold complex 3.Above-mentioned soft tissue window anchoring part 2 is press-fit on the cell-scaffold complex 3; Soft tissue window anchoring part 2 is H4 with the height of cell-scaffold complex 3 intersection; Make between cell-scaffold complex 3 and the soft tissue window 1 and form the gap; Supply new vessels network and soft tissue to grow into, good nutrition supply and metabolism environment is provided for transplanted cells growth and freshman bone tissue form.
Operation of the present invention is as shown in Figure 3: at first according to the virtual condition of bone damaged 4; Select the soft tissue window 1 of fit hole diameter; And it is embedded in (a step among Fig. 3) on the soft tissue window anchoring part 2; The soft tissue window anchoring part 2 of organizing window 1 will be installed again be press-fit in cell-scaffold complex 3 (the b step among Fig. 3) top, assembling is positioned over damaged 4 places of bone (the c step among Fig. 3) with the controlled vascularization artificial bonegraft of processing and repairs after accomplishing.
Need to prove that in the foregoing description, cell-scaffold complex 3 also can be selected any medical material for use, is not limited to titanium alloy.Soft tissue window 1 and soft tissue window anchoring part 2 also can select any medical absorbable material for use, also be not limited to adopt polycaprolactone, every employing structure of the present invention all should belong to protection scope of the present invention.
Claims (6)
1. controlled vascularization artificial bonegraft; It is characterized in that; This controlled vascularization artificial bonegraft is cooperatively interacted by soft tissue window (1), soft tissue window anchoring part (2) and cell-scaffold complex (3) and becomes one; Wherein, the material of soft tissue window (1) and soft tissue window anchoring part (2) is medical absorbable material, and the material of cell-scaffold complex (3) is a medical alloy; The shape of described soft tissue window (1) is a thin rounded flakes, and thin rounded flakes central authorities have circular adjustable through hole; The shape of described soft tissue window anchoring part (2) is an annulus, and soft tissue window (1) is embedded on the soft tissue window anchoring part (2); Described cell-scaffold complex (3) is the porous circular cylinder shape, and the cell-scaffold complex has equally distributed osteoblast in (3), and cell density is 4 * 10
4/ mm
2, cell-scaffold complex (3) and soft tissue window anchoring part (2) connect together, and form the gap between cell-scaffold complex (3) and the soft tissue window (1).
2. controlled vascularization artificial bonegraft as claimed in claim 1 is characterized in that described medical alloy matter is titanium alloy.
3. controlled vascularization artificial bonegraft as claimed in claim 2 is characterized in that the material of described titanium alloy is Ti-6Al-4V.
4. controlled vascularization artificial bonegraft as claimed in claim 1 is characterized in that described medical absorbable material is a polycaprolactone.
5. controlled vascularization artificial bonegraft as claimed in claim 1 is characterized in that the diameter range of the adjustable through hole of described circle is 2mm-10mm.
6. controlled vascularization artificial bonegraft as claimed in claim 1; It is characterized in that the pore morphology of described cell-scaffold complex (3) is connection rate 100% between the space network of simulation C60 former subspace formation, aperture 1000 μ m, porosity 70%, hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110262436.4A CN102415922B (en) | 2011-09-06 | 2011-09-06 | Controllable vascularized artificial bone graft |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110262436.4A CN102415922B (en) | 2011-09-06 | 2011-09-06 | Controllable vascularized artificial bone graft |
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| Publication Number | Publication Date |
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| CN102415922A true CN102415922A (en) | 2012-04-18 |
| CN102415922B CN102415922B (en) | 2014-01-01 |
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| CN201110262436.4A Expired - Fee Related CN102415922B (en) | 2011-09-06 | 2011-09-06 | Controllable vascularized artificial bone graft |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106061438A (en) * | 2013-12-30 | 2016-10-26 | 纽约干细胞基金会 | Tissue grafts and methods of making and using the same |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304711A (en) * | 2000-12-15 | 2001-07-25 | 华西医科大学附属第一医院 | Bio-derivative tissue engineering bone and its preparing process |
| CN1972644A (en) * | 2003-11-21 | 2007-05-30 | 骨棚国际私人有限公司 | Bioabsorbable plug implants and method for bone tissue regeneration |
| US20080077251A1 (en) * | 1999-06-07 | 2008-03-27 | Chen Silvia S | Cleaning and devitalization of cartilage |
| US20080154379A1 (en) * | 2006-12-22 | 2008-06-26 | Musculoskeletal Transplant Foundation | Interbody fusion hybrid graft |
| CN202223387U (en) * | 2011-09-06 | 2012-05-23 | 中国人民解放军第四军医大学 | Controllable vascularization artificial bone graft |
-
2011
- 2011-09-06 CN CN201110262436.4A patent/CN102415922B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080077251A1 (en) * | 1999-06-07 | 2008-03-27 | Chen Silvia S | Cleaning and devitalization of cartilage |
| CN1304711A (en) * | 2000-12-15 | 2001-07-25 | 华西医科大学附属第一医院 | Bio-derivative tissue engineering bone and its preparing process |
| CN1972644A (en) * | 2003-11-21 | 2007-05-30 | 骨棚国际私人有限公司 | Bioabsorbable plug implants and method for bone tissue regeneration |
| US20080154379A1 (en) * | 2006-12-22 | 2008-06-26 | Musculoskeletal Transplant Foundation | Interbody fusion hybrid graft |
| CN202223387U (en) * | 2011-09-06 | 2012-05-23 | 中国人民解放军第四军医大学 | Controllable vascularization artificial bone graft |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106061438A (en) * | 2013-12-30 | 2016-10-26 | 纽约干细胞基金会 | Tissue grafts and methods of making and using the same |
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| CN102415922B (en) | 2014-01-01 |
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Granted publication date: 20140101 Termination date: 20180906 |