CN102408984A - Photo-bioreactor - Google Patents
Photo-bioreactor Download PDFInfo
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- CN102408984A CN102408984A CN2010102958394A CN201010295839A CN102408984A CN 102408984 A CN102408984 A CN 102408984A CN 2010102958394 A CN2010102958394 A CN 2010102958394A CN 201010295839 A CN201010295839 A CN 201010295839A CN 102408984 A CN102408984 A CN 102408984A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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Abstract
The invention discloses a photo-bioreactor. The photo-bioreactor comprises at least two flexible films and support pieces, wherein the flexible films are stacked at intervals and bent back and forth multiple times from top to bottom, thus defining an independent communicating chamber which is bent back and forth from top to bottom between any two adjacent flexible films; a photo-biological culture solution is contained in at least one chamber; and the support pieces are used for supporting the flexible films. Therefore, compared with the bag containers in the prior art, the photo-bioreactor has the following beneficial effects: the photo-bioreactor has a simpler structure, i.e., only a plurality of flexible films are provided, so the photo-bioreactor is very convenient to clean; and in the photo-bioreactor, the shapes and positions of the support pieces can be adjusted, thus conveniently changing the shapes and photic degrees of the culture containers formed by the flexible films and simultaneously facilitating the harvest of the photo-biological culture solution.
Description
Technical field
The present invention relates to photo bio culture technique field, refer to a kind of film light bio-reactor especially.
Background technology
Patent document (publication number WO 2008/151376 A1) discloses a kind of bag film reactor drum, and still, there is following shortcoming in this bag film reactor drum:
The structural unit size of a, reactor drum can't be adjusted after confirming;
B, manufacture craft are comparatively complicated, and each side, bag film unit is wavy, needs hot pick or other auxiliary processes;
C, because the reactor drum width is less, simultaneously wavy sidewall causes internal run-through property relatively poor, and the dead angle is arranged, and cleans very inconvenient;
D, since this reactor drum side be main sensitive surface, reactor drum is put form (direction) and is restricted;
E, reactor drum are darker, and the stress distribution on the side plate is uneven, and material top mechanical property utilization ratio is low.Simultaneously, hydraulic pressure is higher to the material performance requirement of membrane wall.Material cost increases.
Summary of the invention
The objective of the invention is to solve at least one that exists in the prior art in the above-mentioned technical problem.
According to an aspect of the present invention; A kind of bioreactor is provided; Comprise at least two fexible films; Said at least two fexible film space laminated and back and forth bending, thus make and limit an independently reciprocal connection chamber of bending from top to bottom between any two adjacent fexible films, and at least one chamber, hold the photo bio nutrient solution; And strut member, said at least two fexible films of said supports support.
According to a preferred embodiment of the present invention, said strut member comprises curved pipe, and said curved pipe supports said fexible film, and it is container-like that said fexible film is constrained to deep-slotted chip breaker.
According to a further advantageous embodiment of the invention, said strut member also comprises supporting network, and said supporting network is placed on the said curved pipe, is used to prevent said fexible film generation viscous deformation.
According to a further advantageous embodiment of the invention, when being formed with a plurality of independent cavity, hold similar and different photo bio nutrient solution in each independent cavity.
According to a further advantageous embodiment of the invention, when being formed with a plurality of independent cavity, hold the photo bio nutrient solution in some chambers, hold the adjacent chamber of the chamber of photo bio nutrient solution as cooling or heated chamber with these.
According to a further advantageous embodiment of the invention; When results photo bio nutrient solution; Each chamber that tilts down flows out the photo bio nutrient solution from top to bottom in layer, thereby makes two fexible films that constitute each chamber alternately bear the photo bio nutrient solution.
According to a further advantageous embodiment of the invention, said at least two fexible films back and forth are bent into a plurality of layers of group, and each layer component is not by one group of supports support.
According to a further advantageous embodiment of the invention, said every group of strut member comprises: first back up pad and second back up pad, and said first, second back up pad (lays respectively at two vertical end places of the integral support of bioreactor; First curved pipe and second curved pipe, said first, second curved pipe are separately fixed on said first, second back up pad; And supporting network, said supporting network is placed on said first, second curved pipe, is used to prevent said fexible film generation viscous deformation.Wherein, the height of at least one in said first, second back up pad can be regulated, thereby can regulate the obliquity of each layer group of said at least two fexible films.
According to a further advantageous embodiment of the invention, said bioreactor also comprises lifting device, is used to regulate the height of said first back up pad and/or second back up pad.
According to a further advantageous embodiment of the invention; When results photo bio nutrient solution; Through lifting device each chamber is adjusted to heeling condition, thereby makes the photo bio nutrient solution in layer down to flow out, thereby accomplish results from each independent cavity.
According to a further advantageous embodiment of the invention, the radian of each curved pipe can be regulated, thereby changes the shape of the fexible film on it.
According to a further advantageous embodiment of the invention, the radian of each curved pipe is in 0～180 degree scope.
According to a further advantageous embodiment of the invention, the radian of each curved pipe (31a, 31b) can be identical or different.
According to a further advantageous embodiment of the invention, the height spacing between any two neighbouring curved pipes can be regulated, thereby can change the shading degree between the fexible film.
According to a further advantageous embodiment of the invention, any two groups of adjacent strut members can stagger in the horizontal, thereby can change the shading degree between the fexible film.
According to a further advantageous embodiment of the invention, said bioreactor also comprises injection tube, is used for injecting the photo bio nutrient solution to said a plurality of independent cavity.
According to a further advantageous embodiment of the invention, said bioreactor also comprises inlet pipe, is used for to photo bio nutrient solution sparging gases, and said gas comprises the required reactant gases of photo bio growth.
According to a further advantageous embodiment of the invention; Said bioreactor also comprises pump; The photo bio nutrient solution that said pump is cultivated photo bio in the liquid pool is pumped in each independent cavity of said bioreactor; The photo bio nutrient solution in layer down flows out and turns back to said photo bio along the chamber that tilts to be cultivated in the liquid pool, thereby realizes circulating cultivation.
According to a further advantageous embodiment of the invention, the width of the said fexible film of each sheet can be identical, also can be different.
According to a further advantageous embodiment of the invention, said fexible film is selected the film of following material for use: polythene PE film, polyvinylchloride film or polyurethane PU film.
In the present invention; At least two fexible film space laminated and reciprocal bending; Thereby make between any two adjacent fexible films to limit an independently reciprocal connection chamber of bending from top to bottom, and at least one chamber, hold the photo bio nutrient solution.Therefore, compare with bag-type container of the prior art, structure is more simple, only is the multi-disc fexible film, and like this, it is also very convenient to clean.And in the present invention, the shape of strut member and position can be regulated, thereby can change the shape of the formed culture vessel of fexible film easily and receive the light path degree, simultaneously, also are convenient to the results of photo bio nutrient solution.
Description of drawings
Fig. 1 shows bioreactor one-piece construction synoptic diagram according to a preferred embodiment of the present invention;
Fig. 2 shows bioreactor side-view according to a preferred embodiment of the present invention;
Fig. 3 shows liquid mobile synoptic diagram in a plurality of chambers of Fig. 2;
Fig. 4 shows the supporting structure synoptic diagram of fexible film;
Fig. 5 shows the synoptic diagram that is connected between curved pipe and the back up pad;
Fig. 6 shows the synoptic diagram of the spacing between the radian of regulating curved pipe and the neighbouring curved pipe;
Fig. 7 shows circular form bioreactor according to a preferred embodiment of the present invention.
Embodiment
Pass through embodiment below, and combine accompanying drawing, do further bright specifically technical scheme of the present invention.In specification sheets, same or analogous drawing reference numeral is indicated same or analogous parts.Following explanation to embodiment of the present invention is intended to present general inventive concept of the present invention is made an explanation with reference to accompanying drawing, and is not to be understood that to a kind of restriction of the present invention.
Fig. 1 shows bioreactor one-piece construction synoptic diagram according to a preferred embodiment of the present invention.As shown in Figure 1, in a preferred embodiment of the invention, bioreactor mainly comprises at least two fexible films 2 and the strut member 3 that supports at least two fexible films 2.
Fig. 2 shows bioreactor side-view according to a preferred embodiment of the present invention.Fig. 3 shows liquid mobile synoptic diagram in a plurality of chambers of Fig. 2.
As shown in Figures 2 and 3; Four fexible film 2a, 2b, 2c, 2d range upon range of at interval in the upper and lower and reciprocal bending along the short transverse shown in Fig. 1 (vertical direction), thus make and limit an independently reciprocal connection chamber of bending from top to bottom between any two adjacent fexible films.Therefore, as shown in Figure 3, between four fexible film 2a, 2b, 2c, 2d, define three independent cavity f1, f2, f3 altogether.In these independent cavity f1, f2, f3, can hold the photo bio nutrient solution.
Although in Fig. 2 and Fig. 3, shown four fexible film 2a, 2b, 2c, 2d,, note that the present invention is not limited to this, two, three, five or multi-disc fexible film more also can be set.
Fig. 4 shows the supporting structure synoptic diagram of fexible film.As shown in Figure 4, strut member 3 comprises curved pipe 31a, 31b, curved pipe 31a, 31b supporting flexible film 2, and it is container-like that fexible film 2 is constrained to deep-slotted chip breaker.
Preferably, as shown in Figure 4, strut member 3 also comprises supporting network 32, and supporting network 32 is placed on curved pipe 31a, 31b is last, and 2 of fexible films are placed on the supporting network 32, thereby prevents fexible film 2 from viscous deformation taking place and damage.But, need to prove, supporting network 32 not necessarily, the liquid that on fexible film 2, holds can not adopt supporting network 32 more fully.
As shown in Figure 3, because four fexible film 2a, 2b, 2c, 2d have formed three independent cavity f1, f2, f3, therefore, can hold similar and different photo bio nutrient solution at each independent cavity f1, f2, f3.
As shown in Figure 3; Because four fexible film 2a, 2b, 2c, 2d have formed three independent cavity f1, f2, f3; Therefore; Also can be only in middle chamber f2, hold the photo bio nutrient solution, and on be adjacent chamber f1 and/or below feed fluid or gas cold or heat among a chamber f3, come to cool off or heat to holding the photo bio nutrient solution among the middle chamber f2.
As shown in Figure 3; When results photo bio nutrient solution; Can the photo bio nutrient solution down be flowed out from top to bottom in layer, shown in the arrow among Fig. 3 through each chamber f1, f2, the f3 of tilting; Like this, just can make two fexible films that constitute each chamber alternately bear the photo bio nutrient solution.
As shown in Figures 2 and 3, in one embodiment of the invention, four fexible films from top to bottom back and forth bending four times, formed five layer groups 21,22,23,24,25 altogether, each layer group self is divided into four layers again.
As shown in Figure 2, each layer component is not by one group of supports support.Every group of strut member comprises a pair of first back up pad 11a and a pair of second back up pad 11b.The first back up pad 11a and the second back up pad 11b lay respectively at two vertical end places of the integral support 1 of bioreactor.Strut member also comprises the first curved pipe 31a and the second curved pipe 31b, and the first curved pipe 31a and the second curved pipe 31b are separately fixed on the first back up pad 11a and the second back up pad 11b.Similar with Fig. 4, on the every couple first curved pipe 31a and the second curved pipe 31b, also be placed with supporting network 32, be used to prevent that fexible film 2a, 2b, 2c, 2d from viscous deformation taking place and damage.
Fig. 5 shows the synoptic diagram that is connected between curved pipe and the back up pad.As shown in Figure 5, insert in the jack on a pair of first back up pad 11a at the two ends of the first curved pipe 31a, and insert in the jack on a pair of second back up pad 11b at the two ends of the second curved pipe 31b.
As shown in Figure 1, bioreactor also comprises lifting device 6, is used to regulate the height of the first back up pad 11a and/or the second back up pad 11b, thereby can regulate the obliquity of each layer group of four fexible films.But, need to prove that lifting device 6 is not necessarily cultured when requiring when the vertical thin film layer inclination angle that is provided with in advance reaches to gather to require to satisfy simultaneously, can not adopt lifting device 6 fully, thus simplified construction.
As shown in Figures 2 and 3, in one embodiment of the invention, through regulating lifting device 6; Make that layer group 21 is downward-sloping towards the left side, layer group 22 is downward-sloping towards the right side, and layer group 23 is downward-sloping towards the left side; Layer group 24 is downward-sloping towards the right side, and layer group 25 is downward-sloping towards the left side.When a plurality of layers of group 21,22,23,24,25 when being in heeling condition shown in Figure 3, the photo bio nutrient solution among three independent cavity f1, f2, the f3 just can flow out from outlet in layer toward dirty at last, thus the results of completion photo bio nutrient solution.
In a preferred embodiment of the invention, lifting device 6 can be selected any suitable device in the prior aries such as cam gear, pulley gear and wheelwork for use.
Fig. 6 shows the synoptic diagram of the height spacing between the radian of regulating curved pipe and the neighbouring curved pipe.As shown in Figure 6, the radian that supports the curved pipe of a following fexible film 2b is adjusted to the radian greater than the curved pipe that supports a top fexible film 2a.Through changing the radian of neighbouring curved pipe, thereby change on it fexible film 2a that is supported, the shape of 2b, so just can change the shape and the volume of two adjacent fexible film 2a, the chamber between the 2b.In addition, when D among Fig. 6 was 0 left and right sides, the film through selecting wide or different in width (promptly; In the present invention, the width of each sheet fexible film can be identical, also can be different; This can select according to actual needs); And etc. the curved pipe (during the curved pipe of different radians, lower film places on the bigger curved pipe of radian) of radian or different radians, can optionally make selected cavity reach the effect of closed cavity.
In a preferred embodiment of the invention, as shown in Figure 4, the radian of each curved pipe 31a, 31b can change in 0～180 degree scope.
Please continue referring to Fig. 6; The height space D that supports the curved pipe of a following fexible film 2b and support between the curved pipe of a top fexible film 2a is regulated through changing various supports plate (the curved pipe open hole height space D of making in advance as on the 11a is different); (also can pass through special update device, but can increase structure complexity, not recommend this method; So do not draw); When space D increases, block each other and will reduce between neighbouring two fexible film 2a, the 2b, when space D reduces; Block each other and will strengthen between neighbouring two fexible film 2a, the 2b, even the fexible film 2a above occurring covers the extreme case on the following fexible film 2b fully.
Although not shown, in a preferred embodiment of the invention, any two groups of adjacent strut members can stagger in the horizontal, thereby can change the shading degree between the fexible film.
As shown in Figure 1, in a preferred embodiment, bioreactor also comprises injection tube 4, and a plurality of chamber f1, f2, the f3 that are used for constituting to fexible film inject the photo bio nutrient solution.Injection tube 4 is installed and is fixed on the integral support 1.
In the present invention, in order to accelerate the speed of growth of photo bio, bioreactor of the present invention also comprises inlet pipe 5, is used for to photo bio nutrient solution sparging gases, comprises the required reactant gases of photo bio growth in this gas.Like this, not only can be in the photo bio nutrient solution postreaction gas, but also can utilize the gas of injection fully to stir the photo bio nutrient solution, thereby improve the optical efficiency that receives of photo bio nutrient solution.In a preferred embodiment of the invention, in order to reduce manufacturing cost, fexible film is selected the film of following material for use: polythene PE film, polyvinylchloride film or polyurethane PU film.
Fig. 7 shows circular form bioreactor according to a preferred embodiment of the present invention.As shown in Figure 7; Pump 8 is cultivated photo bio among each independent cavity f1 that photo bio nutrient solution in the liquid pool 7 is pumped into bioreactor, f2, the f3; The photo bio nutrient solution in layer down flows out and turns back to photo bio along the chamber f1, f2, the f3 that tilt to be cultivated in the liquid pool 7, thereby realizes circulating cultivation.
Though describe the present invention in conjunction with accompanying drawing, disclosed embodiment is intended to the preferred embodiment for the present invention is carried out exemplary illustration in the accompanying drawing, and can not be interpreted as a kind of restriction of the present invention.
Though some embodiment of this present general inventive concept are shown and explain; Those skilled in the art will appreciate that; Under the situation of principle that does not deviate from this present general inventive concept and spirit, can make a change these embodiment, scope of the present invention limits with claim and their equivalent.
Claims (20)
1. a bioreactor is characterized in that, comprising:
At least two fexible films (2a, 2b, 2c, 2d); Said at least two fexible film space laminated and reciprocal from top to bottom bending are repeatedly; Thereby make between any two adjacent fexible films to limit an independently reciprocal connection chamber (f1, f2, f3) of bending from top to bottom, and at least one chamber, hold the photo bio nutrient solution; With
Strut member (3), said strut member (3) supports said at least two fexible films.
2. bioreactor according to claim 2 is characterized in that, said strut member (3) comprising:
Curved pipe (31a, 31b), said curved pipe (31a, 31b) supports said fexible film, and it is container-like that said fexible film is constrained to deep-slotted chip breaker.
3. bioreactor according to claim 2 is characterized in that, said strut member (3) also comprises:
Supporting network (32), said supporting network (32) are placed on the said curved pipe (31a, 31b), are used to prevent said fexible film generation viscous deformation.
4. bioreactor according to claim 1 is characterized in that, when being formed with a plurality of independent cavity (f1, f2, f3), holds similar and different photo bio nutrient solution in each independent cavity (f1, f2, f3).
5. bioreactor according to claim 1; It is characterized in that; When being formed with a plurality of independent cavity (f1, f2, f3), hold the photo bio nutrient solution in some chambers, hold the adjacent chamber of the chamber of photo bio nutrient solution as cooling and heated chamber with these.
6. bioreactor according to claim 1; It is characterized in that when results photo bio nutrient solution, each chamber tilts; The photo bio nutrient solution is down flowed out from top to bottom in layer, thereby make two fexible films that constitute each chamber alternately bear the photo bio nutrient solution.
7. bioreactor according to claim 1 is characterized in that, said at least two fexible films back and forth are bent into a plurality of layers of group (21,22,23,24,25) from top to bottom, and each layer component is not by one group of supports support.
8. bioreactor according to claim 7 is characterized in that, said every group of strut member comprises:
First back up pad (11a) and second back up pad (11b), said first, second back up pad (11a, 11b) lay respectively at two vertical end places of the integral support (1) of bioreactor;
First curved pipe (31a) and second curved pipe (31b), said first, second curved pipe (31a, 31b) are separately fixed on said first, second back up pad (11a, 11b); With
Supporting network (32), said supporting network (32) are placed on said first, second curved pipe (31a, 31b), are used to prevent said fexible film generation viscous deformation,
Wherein, the height of at least one in said first, second back up pad (11a, 11b) can be regulated, thereby can regulate the obliquity of each layer group of said at least two fexible films.
9. bioreactor according to claim 8 is characterized in that, said bioreactor also comprises lifting device (6), is used to regulate the height of said first back up pad (11a) and/or second back up pad (11b).
10. bioreactor according to claim 9 is characterized in that,
When results photo bio nutrient solution, through lifting device (6) each chamber is adjusted to heeling condition, thereby makes the photo bio nutrient solution in layer down to flow out, thereby accomplish results from each independent cavity (f1, f2, f3).
11. bioreactor according to claim 10 is characterized in that, the radian of each curved pipe (31a, 31b) can be regulated, thereby changes the shape of the fexible film on it.
12. bioreactor according to claim 11 is characterized in that, the radian of each curved pipe (31a, 31b) is in 0～180 degree scope.
13. bioreactor according to claim 12 is characterized in that, the radian of each curved pipe (31a, 31b) can be identical or different.
14. bioreactor according to claim 12 is characterized in that, the height spacing between any two neighbouring curved pipes (31a, 31b) can be regulated, thereby can change the shading degree between the fexible film.
15. bioreactor according to claim 7 is characterized in that, any two groups of adjacent strut members can stagger in the horizontal, thereby can change the shading degree between the fexible film.
16. bioreactor according to claim 1 is characterized in that, also comprises injection tube (4), is used for injecting the photo bio nutrient solution to said a plurality of independent cavity.
17. bioreactor according to claim 1 is characterized in that, also comprises inlet pipe (5), is used for to photo bio nutrient solution sparging gases, said gas comprises the required reactant gases of photo bio growth.
18. bioreactor according to claim 1 is characterized in that, also comprises:
Pump (8); The photo bio nutrient solution that said pump (8) is cultivated photo bio in the liquid pool (7) is pumped in each independent cavity (f1, f2, f3) of said bioreactor; The photo bio nutrient solution in layer down flows out and turns back to said photo bio along the chamber (f1, f2, f3) that tilts to be cultivated in the liquid pool (7), thereby realizes circulating cultivation.
19., it is characterized in that the width of the said fexible film of each sheet (2a, 2b, 2c, 2d) can be identical or different like each described bioreactor among the claim 1-18.
20. like each described bioreactor among the claim 1-18, it is characterized in that said fexible film is selected the film of following material for use: polythene PE film, polyvinylchloride film or polyurethane PU film.
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| CN201010295839.4A CN102408984B (en) | 2010-09-21 | 2010-09-21 | Photo-bioreactor |
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| CN104968777A (en) * | 2013-01-29 | 2015-10-07 | 帕特艾菲克特专利权管理民法公司 | Bioreactor suspension |
| CN112849640A (en) * | 2020-12-28 | 2021-05-28 | 罗叶海 | Support for supporting operation of pipeline type photobioreactor |
| CN113862111A (en) * | 2021-08-31 | 2021-12-31 | 国核自仪系统工程有限公司 | Microalgae cultivation device and method for reducing water eutrophication |
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| CN113862111B (en) * | 2021-08-31 | 2023-11-07 | 国核自仪系统工程有限公司 | Microalgae cultivation device and method for reducing water eutrophication |
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