CN102397317A - Method for producing soybean Genistin and application of soybean Genistin in preparation of estrogenic drugs or female health food - Google Patents
Method for producing soybean Genistin and application of soybean Genistin in preparation of estrogenic drugs or female health food Download PDFInfo
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Abstract
The invention relates to a method for producing soybean Genistin and an application of the soybean Genistin in preparation of estrogenic drugs or female health food, which belongs to the drugs and health food fields. The invention confirms that the soybean Genistin is an effective component in soybean isoflavone, and the invention provides a method for producing high content and low content soybean composite extracts of the soybean Genistin. The application of the soybean Genistin in preparation of estrogenic drugs or female health food for improving female secondary sex characteristics growth, preventing and treating female menopausal syndrome, preventing female senile dementia and stature atrophy, preventing and treating cancers relative with estrogenic ecretion and the like can be confirmed, and the effective and ineffective formula application dosages for different diseases can be also confirmed, the technical misunderstanding that soybean isoflavone is used for health care is overcome, and the invention provides a novel raw material substrate for researching novel natural plant estrogen drugs and health food. The invention provides an effective formula dosage for improving female secondary sex characteristics growth, preventing and treating female menopausal syndrome, preventing female senile dementia and stature atrophy, preventing and treating cancers and the diseases relative with estrogenic ecretion.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to the application of the female graceful production method of Semen sojae atricolor Jie girl and preparation type of having estrogen function medicine or health food.
Background technology
In recent years, soybean isoflavone is widely used in improving the growth of women's secondary sexual characteristics, is prevented and treated climacteric syndrome, is prevented dull-witted relevant with height atrophy, control and the estrogen secretion cancer of female old aged people etc. as natural plant estrogen both at home and abroad.But after soybean isoflavone oral crowd in commercial goods takes in, often find drug effect and not obvious, even invalid phenomenon.
In the soybean isoflavone; Had at least 12 kinds by the isomer of this field approval; Find after deliberation; In the various isomers in the soybean isoflavone, most to above-mentioned disease type of nothing estrogen effect, as one of main component in the existing industrial soybean isoflavone isomer---daiazi is the not estrogenic effect of type of having basically.In the industrial soybean isoflavone isomer, mainly the estrogenic functional component of type of having is " the Jie girl is female graceful "." the Jie girl is female graceful " is the transliteration title of " Genistin " and " Genistein " two kinds of isomers; Because above two kinds of isomer transliteration pronunciation is basic identical; And equal type of having estrogen action; Therefore in the present invention, be " the Jie girl is female graceful " with the unified transliteration of " Genistin " and " Genistein ", the Chinese translation of " Genistin " is also claimed genistin or genistein; The Chinese translation of " Genistein " also has the appellation of dyewood aglycon or genistein; But these translated names are in application; The exposed population group is generally reflected obscure; Be the class estrogen function of reflection " Genistin " and " Genistein ", transliteration " the Jie girl is female graceful " that it is vivid is as the title of product among the present invention.
The purity of at present commercially available soybean isoflavone mark means the comprehensive total content of the various isomers of soybean isoflavone; Do not spell out the female graceful content of Jie girl, this patent finds when using, and no matter contains the prescription compatibility how of medicine or the health food of soybean isoflavone; As take the people and take in the female graceful dosage of pure Jie girl＜6mg/ day for human beings every day; Then type estrogen action is not obvious, even type of nothing estrogen effect, thereby causes soybean isoflavone health care prestige to descend; Price reduces significantly, and the soybean isoflavone whole industry faces the threat of hurried decline.
Summary of the invention
The purpose of this invention is to provide the female graceful method for distilling of a kind of Semen sojae atricolor Jie girl, make it in the Semen sojae atricolor composite extract, needed content ratio can be arranged.
Another purpose of the present invention provides the female graceful purposes in medicine or health food of a kind of Semen sojae atricolor Jie girl.
A further object of the present invention is that to establish Semen sojae atricolor Jie girl female graceful at the dose when improving women's secondary sexual characteristics and grow, prevent and treat type estrogenic or the health food of dull-witted relevant with height atrophy, control and the estrogen secretion cancer of climacteric syndrome, prevention female old aged people.
The female graceful production method of low-purity Semen sojae atricolor Jie girl of the present invention is:
1, is raw material with bean cake or soybean seed or soybean cake, is ground into 20～100 purpose powder;
2, the powder that step 1 is obtained is packed in the lixiviate jar of belt stirrer; In the lixiviate jar, add concentration and be 20%～80% natural gas level methanol (or hot water of the female graceful solvent of other leachable Jie girl such as ethanol or methanol and alcohol mixeding liquid or acetone or ethyl acetate or 80 °～100 ℃); The adding quantity of solvent is 7～12 times of grain weight amount; Fully stir, mixing speed is 60～80r/min, mixing time 6～12h;
3, will pass through the mixed liquor that step 2 obtains and filter through 50～200 order filters, the material conveying pump power is 10KW, and operating pressure is 0.4～0.6Mpa;
4, be that 100KW, rotating speed are the automated friction desolventizing machine exsolution drying of 1450r/min through step 3 isolated by filtration gained bean dregs through power, with solvent recovery, bean dregs moisture is reduced to below 7%;
5, step 3 is carried out microporous filter through the filtrating that filter obtains, reach transparency, filter the order number and be generally 50～200 orders, operating pressure is 0.2～0.6Mpa;
6, the transparent feed liquid that step 5 is obtained is sent into the feed liquid electrical conductivity that electrodialysis plant removes behind inorganic salt, the desalination should＜50 μ s/cm
2
7, will transparent filtrating distill concentrated, recovery solvent through the desalination that step 6 obtains, the distillating recovering solvent temperature remains on 100 °～120 ℃, and distillation time is 20～60min;
8, the transparent feed liquid that step 7 distillating recovering solvent handles be will pass through and 135 ℃ of high-temperature instantaneous sterilization devices, sterilizing time 5～7S pumped into; Pumping into steam pressure again is that 180Kpa, vacuum are that 90Kpa, temperature are 55 ℃ enrichment facility; Concentrated solution concentration reaches at 10%～20% o'clock, and directly fill can be produced and contained the female graceful liquid Semen sojae atricolor composite extract (by the ratio of butt) of 0.01%～0.5% Jie girl.Maybe will be concentrated into 10%～20% concentrated solution and pump in the press spray tower, inlet temperature is 160 °～180 ℃, and outlet temperature is 60 °～80 ℃, and the gained powder product is the Semen sojae atricolor composite extract that contains the female graceful amount about 0.1%～5.0% of Jie girl after the spray drying.
In the Semen sojae atricolor composite extract owing to wherein also contain water-solubility protein, nucleic acid, folic acid, oligosaccharide etc.; So be called " Semen sojae atricolor composite extract "; The female graceful detection method of Jie girl adopts NT/T1252-2006 Ministry of Agriculture ministry standard to detect in the Semen sojae atricolor composite extract, sees the 9th～11 page of this description for details.
The female graceful production method of high-purity soybean Jie girl of the present invention is:
1, is raw material with bean cake or soybean seed or soybean cake, is ground into 20～100 purpose powder;
2, the powder that step 1 is obtained is packed in the lixiviate jar of belt stirrer; In the lixiviate jar, add concentration and be 20%～80% natural gas level methanol (or hot water of the female graceful solvent of other leachable Jie girl such as ethanol or methanol and alcohol mixeding liquid or acetone or ethyl acetate or 80 °～100 ℃); The adding quantity of solvent is 7～12 times of grain weight amount; Fully stir, mixing speed is 60～80r/min, mixing time 6～12h;
3, will pass through the mixed liquor that step 2 obtains and filter through 50～200 order filters, the material conveying pump power is 10KW, and operating pressure is 0.4～0.6Mpa;
4, be that 100KW, rotating speed are the automated friction desolventizing machine exsolution drying of 1450r/min through step 3 isolated by filtration gained bean dregs through power, with solvent recovery, bean dregs moisture is below 7%;
5, step 3 is carried out microporous filter through the filtrating that filter obtains, reach transparency, filtering the order number is 50～200 orders, and operating pressure is 0.2～0.6Mpa;
6, with the transparent filtered solution after the distillation of step 5 gained, remove inorganic salt through electrodialysis, the feed liquid electrical conductivity behind the desalination must not surpass 50 μ s/cm
2
7, will pass through macroporous adsorbent resin through the feed liquid behind step 6 desalination; Contained female graceful, the soybean saponin of Jie girl in the material of resin absorption; Effusive feed liquid is a soybean oligo saccharide; After absorption with macroporous adsorbent resin is saturated, molten from eluting with 20%～80% natural gas level methanol (or hot water of ethanol or methanol and alcohol mixeding liquid or acetone or ethyl acetate or 80 °～100 ℃);
8, the eluent that step 7 is obtained is regained solvent through distilling to concentrate;
9, will be through eluent thin up to the concentration 2%～10% of step 8 concentrated solution;
10, can water-soluble, the female graceful biological characteristics that is insoluble to normal-temperature water of Jie girl according to saponin, the solution liquid that step 9 is obtained adopts the high speed separator that is higher than 10000 rev/mins to separate to such an extent that contain female graceful solid phase Semen sojae atricolor composite extract of Jie girl and the supernatant that contains saponin; Contain the female graceful solid phase Semen sojae atricolor of Jie girl extract complex by 9,10 steps dilute repeatedly, separating treatment 2～5 times, different according to number of processes, can obtain containing the Semen sojae atricolor composite extract (by the ratio of butt) of the female graceful different purity of 5%～95% Jie girl;
11, with the female graceful solid phase Semen sojae atricolor composite extract thin up of step 10 gained Jie girl to solid content about 10%; After sterilization, spray drying is the female graceful powder product of Jie girl; Product purity can because of step 9,10 dilution and to separate number of times different, obtain containing the female graceful powdery soybean composite extract of 5%～95% Jie girl.The female graceful method for detecting purity of Jie girl is seen the 9th～11 page of description of the present invention.
The female graceful purposes of Semen sojae atricolor Jie girl that provides among the present invention is:
The female graceful application in preparation type estrogenic or health food of Semen sojae atricolor Jie girl.
The female graceful effective dose of the Semen sojae atricolor Jie girl that the present invention provides is:
According to the female graceful different content of Jie girl, the computational methods that provide a kind of calculating to take crowd's everyone effective dose that should take in every day, concrete formula is following:
A＝B÷C÷D
A: should take in the Semen sojae atricolor composite extract manufactured goods of Semen sojae atricolor composite extract composition (or contain) dosage (gram) everyone every day;
B: have the effective dose that the Jie girl of medical treatment or health-care efficacy should take in female graceful everyone every day according to experiment proof;
C: the female graceful content ratio of Jie girl in the Semen sojae atricolor composite extract of the practical application manufactured goods of Semen sojae atricolor composite extract composition (or contain);
D: it is 1000mg/g that every gram is amounted to milligram number.
For example: proof is used to prevent and treat climacteric syndrome after deliberation; 50 years old left and right sides women; Should every day everyone to take in 6mg Jie girl female graceful; The ratio that the female graceful content of Jie girl accounts in the product in the product that employing the present invention produces is 0.2mg/g, then should take in the commodity dosage (gram) of this kind Semen sojae atricolor composite extract everyone every day:
A=6mg/ day for human beings ÷ 0.2mg/g ÷ 1000mg/g
=6mg/ day for human beings * 1000mg/0.2mg ÷ 1000mg/g
=30 gram/day for human beings
That is: be the control climacteric syndrome, should take in everyone every day contain the Jie girl female graceful for the commodity soybean composite extract amount of 0.2mg/g be 30 to restrain.
And for example for preventing and treating women's cancer (like breast carcinoma); Through experiment showed, that the patient takes in female graceful the answering＞200mg of Jie girl everyone every day, adopts in the product of the present invention's production; The female graceful content ratio of Jie girl is as being 40%, and the amount that then should take in this kind Semen sojae atricolor composite extract everyone every day is:
A=200mg/ day for human beings ÷ 40% ÷ 1000mg/g
=0.5 gram
That is: be that it is that 40% the female graceful amount of Jie girl should be no less than 0.5 gram that control women breast cancer, patient should be taken in purity everyone every day.
The female graceful detection method of content of above-mentioned Jie girl all adopts Ministry of Agriculture's ministry standard (NT/T1252-2006) to detect, and sees 9～11 pages of description of the present invention for details.
Positive role of the present invention is to find that the isomer composition that soybean isoflavone that present industrialization is extracted really has an estrogen action is that the Jie girl is female graceful; And provide according to this discovery and to have obtained the female graceful production method of Semen sojae atricolor Jie girl; The clear and definite female graceful class estrogen purposes in medicine or health food of Semen sojae atricolor Jie girl; The computational methods of taking in effective dose everyone every day of indication have been provided; Overcome the misunderstanding of generally preventing and treating above-mentioned disease with soybean isoflavone; Semen sojae atricolor Jie girl is female graceful as after a kind of natural plant estrogen suitability for industrialized production, can increase substantially the benefit of soybean processing industry, for development of new natural plant estrogen new drug and health product provide the novel material material.Grow, prevent and treat climacteric syndrome for improving women's secondary sexual characteristics, prevent the dull-witted diseases such as cancer relevant of female old aged people that the effective application approach is provided with height atrophy, control and estrogen secretion.
Female graceful product of Semen sojae atricolor Jie girl and medical treatment, health-care effect checking.
The crowd of participating in the experiment is 60 of 46 years old～70 years old, healthy women; Be divided into three groups, every group of each 20 people keep each to organize participate in the experiment people's age, body weight basically identical during grouping; " Semen sojae atricolor is extracted complex " of containing daiazi and the female graceful different content of Semen sojae atricolor Jie girl everyone every day; Took continuously 20, before taking 24h and last take back 24h respectively vein get blood, survey serum estradiol (E
2) content, and take the reflection of forward and backward physiology by the women's readme of participating in the experiment, carry out own control, result such as table 1.
Description of drawings
Fig. 1 is the used Semen sojae atricolor composite extract of test I group HPLC spectrogram.
Fig. 2 is the used Semen sojae atricolor composite extract of test II group HPLC spectrogram.
Fig. 3 is the used Semen sojae atricolor composite extract of test III group HPLC spectrogram.
Fig. 4 is the used Semen sojae atricolor composite extract of test IV group HPLC spectrogram.
Fig. 5 is the used Semen sojae atricolor composite extract of the V group HPLC spectrogram that is used for the antitumor test.
Each test group component content HPLC spectrogram is seen accompanying drawing 1,2,3 in the table 1.
Test used Semen sojae atricolor complex assay method employing Ministry of Agriculture's ministry standard " soybean isoflavone assay method " (NT/T 1252-2006) for three groups in the table 1, concrete grammar is following:
1. the method content and the scope of application
This method has been stipulated the detection method of soybean isoflavone aglycones in the soybean extract.
This method is applicable to the detection of soybean isoflavone aglycones in the soybean extract.
2. method summary
It is to utilize various separation component, mobile phase and the C of treating of isoflavone that the HPLC method of soybean isoflavone detects
18The difference of the absorption between the filler, distribution and equilibrium valve makes each component holdup time in chromatographic column different and carry out isolatingly, reaches peak area in chronological order with UV-detector then and carries out qualitative, quantitative analysis.
3. instrument and equipment
A) high performance liquid chromatograph is furnished with UV-detector, column oven and chromatographic work station;
B) ultrasonic wave concussion device;
C) analytical balance: sensibility reciprocal 0.0001g.
4. reagent and standard substance
A) 95% ethanol: analytical pure;
B) acetonitrile: purity 99.9%, chromatographically pure;
C) glacial acetic acid: purity >=99.5%, analytical pure;
D) water: with the filterable distilled water of 0.45 μ m microfilter;
E) daiazi standard substance: content 99%;
F) daidzin standard substance: content 99%;
G) genistin (Genistin is that the Jie girl is female graceful) standard substance: content 99%.
5. chromatographic condition and system suitability experiment
A) chromatographic column: C
18, φ 4.6mm * 250mm, 5 μ m;
B) mobile phase: acetonitrile: water: acetic acid, 11: 89: 0.1 (volume ratio);
C) detect wavelength: UV254nm;
D) flow velocity: 2.0mL/min;
E) column temperature: 40 ℃;
F) sample size: 20 μ L.
For making system's coincidence detection requirement; Be defined under the chromatographic condition; (6 " O-malonyldaidzin) chromatographic peak of component must not overlap each other female graceful, the malonyl daiazi of daiazi and daidzin and Jie girl separately, and other assorted peaks that between above-mentioned four chromatographic peaks, do not have obviously occur.
After malonyl daiazi peak occurs, use B.5b) mobile phase washes post 15min, recover A.5b then) mobile phase balance columns 15min, go up again kind.
6. the preparation of isoflavone standard solution
A) take by weighing daiazi, daidzin, female graceful each the 10mg ± 0.1mg of Jie girl, place the 25mL volumetric flask, with standardize solution behind 80% the dissolve with ethanol.The soybean isoflavone total concentration is 1.2mg/mL in this standard solution, and is subsequent use as mother solution.
B) accurate absorption mother solution 1.0,2.0,3.0,4.0 and 5.0mL solution move to respectively in 5 25mL volumetric flasks, use 80% ethanol standardize solution then.The concentration of above-mentioned 3 kinds of component standard specimens is respectively 0.016,0.032,0.048,0.064 and 0.080mg/mL in 5 gradient titers.
7. sample preparation
A) getting 5g behind the soybean extract sample mixing, to put into exsiccator vacuum drying 24h subsequent use.
B) take by weighing above-mentioned over dry soybean extract sample 25mg ± 0.1mg, place the 25mL volumetric flask, add the about 20mL of 80% ethanol and dissolve the back standardize solution fully.Get 3mL～5mL then, reuse 80% ethanol standardize solution is in the 25mL volumetric flask.Draw 1mL solution in centrifuge tube, with the centrifugal 10min of 10000rpm, it is subsequent use as sample solution to get the supernatant.
8. measure and result's calculating
A) according to chromatographic condition A.5, to A.6b) 5 gradient titers analyze, and corresponding data are imported chromatographic work station.
The sample solution that b) will A.7 prepare is analyzed by chromatographic condition A.5, then by chromatographic work station to the chromatograph of sample and standard sample according to calculating, can draw glycosides in the sample and (comprise the female graceful content G of daiazi and Jie girl
0 1(%).
C) one-bit digital behind the result of calculation reservation arithmetic point.
D) the parallel assay result is with the arithmetic average value representation, relative deviation≤2.5%.
9. expression of results
This mensuration is expressed as the gram number that contains soybean isoflavone in every 100g absolute dried sample.
The composition that has natural plant estrogen effect in this patent inventor's evidence, the Semen sojae atricolor is that Semen sojae atricolor Jie girl is female graceful; Everyone took in 6mg and had remarkable estrogen effect (p＜0.01) adult women every day; When amount＜5.8mg was gone in the female graceful picked-up of Jie girl, the test crowd does not produce the physiology that influenced by estrogen to be reflected, the daiazi intake is from 3.24mg～6.00mg/ day for human beings; Edible test women crowd is not all produced estrogen action, prove the basic no estrogen effect of daiazi; When the female graceful conduct of Jie girl is used to add the interpolation raw material of various health foods or medicine increase estrogen action or eats certain Semen sojae atricolor extraction complex separately; The present invention provides a kind of effective prescription dosage; Be to obtain significant estrogen action effect during the edible female graceful amount of Jie girl >=6.00mg everyone every day; And daiazi type of having estrogen action not so confirming that any Semen sojae atricolor extracts in the prescription of complex, must indicate the female graceful content of Jie girl; During adult women's everyone intake every day >=6.00mg, could produce a type estrogen action.
When the female graceful prescription at class estrogen health food and medicine of research Jie girl was used, this patent found that the Jie girl femalely gracefully is used to improve women's secondary sexual characteristics, prevents and treats climacteric syndrome, the high atrophy of control I aspect is not that the female graceful intake of medical health effect and Jie girl is perfect positive correlation and concerns.
For example: certain Semen sojae atricolor is extracted in the complex and contains Jie girl female graceful 4.2%; Contain daiazi 2.9%; Recommending everyone every day of edible this kind Semen sojae atricolor extraction complex amount is 1200mg; Promptly grow up women every day everyone to take in the female graceful amount of Jie girl be the 50.4mg/ day for human beings, far surpass effective intake 6mg/ day for human beings index that this invention patent is recommended, but not only do not improve downward trend is arranged on the contrary through measuring the serum estradiol content of taking the before and after test crowd.See table 2 for details.
Table 2
Supply in the test agent that the Jie girl is female gracefully to see accompanying drawing 4 with daiazi HPLC spectrogram.
It is thus clear that the female graceful application of Jie girl is not to follow dosage to improve and the enhancing of type of making estrogen; But after human body absorption dosage surpasses type estrogen function virtual value; Can suppress the human body self estrogen function on the contrary, serum estradiol content has downward trend, and promptly Semen sojae atricolor Jie girl is female graceful because intake different; Can produce the two-phase regulating action, promptly low intake (6～50mg/ day for human beings) produces a type estrogen effect; Intake＜6mg/ the day for human beings, type low or no estrogen effect of estrogen effect; High dose Jie girl female graceful＞the 50mg/ day for human beings can be used for the excessive malignant tumor that causes of antagonism animal self estrogen secretion.
The test V group that is used for antagonism animal milk adenocarcinoma.
Be used to prevent and treat the liquid chromatograph spectrogram that the female graceful Semen sojae atricolor of high-purity Jie girl of jenny malignant tumor extracts complex and see accompanying drawing 5.
With 50 of the white II mices in Tianjin of the mammary gland of the in-situ inoculating carcinoma strain of average weight ≈ 20g; Random packet; Wherein matched group is 14; Each 12 of high, medium and low dose groups, add by normally feeding every day contain the Jie girl female graceful be that 92.60% Semen sojae atricolor is extracted complex, be respectively: (1) saline control group; (2) 0.08mg/ day; (3) 0.05mg/ day; (4) 0.03mg/ day.Irritate and feed administration, after 40 days, the stripping tumor, the weighing tumor is heavy.
The female graceful intake of different Jie girls, animal is to the inhibitory action of mastadenoma
As the adult female who is converted into 60 kg body weight answers dosing; Then taking in purity everyone every day is the female graceful 250mg of 92.66% Jie girl, 150mg, 100mg; Being equivalent to the female graceful amount of pure Jie girl is 231.5mg, 138.9mg, 92.6mg, and tumour inhibiting rate can reach 61.3%, 46.3%, 36.0% respectively.
This is tested used Semen sojae atricolor and extracts in the complex; The female graceful content of Jie girl is up to 92.60%; And daiazi content is merely 6.76%, is 7% of the female graceful content of Jie girl, and visible antagonism animal body inner estrogen is secreted excessive and malignant tumor that cause; Main acting composition is that the Jie girl is female graceful, and is irrelevant with the daiazi intake.
Claims (5)
1. the female graceful production method of Semen sojae atricolor Jie girl, its characteristic may further comprise the steps:
(1) is raw material with bean cake or soybean seed or soybean cake, is ground into 20～100 purpose powder;
(2) powder that step (1) is obtained is packed in the lixiviate jar of belt stirrer; In the lixiviate jar, add concentration and be the hot water of 20%～80% natural gas level methanol or ethanol or methanol and alcohol mixeding liquid or acetone or ethyl acetate or 80 °～100 ℃; The adding quantity of solvent is 7～12 times of grain weight amount; Fully stir, mixing speed is 60～80r/min, mixing time 6～12h;
(3) will pass through the mixed liquor that step (2) obtains and filter through 50～200 order filters, the material conveying pump power is 10KW, and operating pressure is 0.4～0.6Mpa;
(4) step (3) is carried out microporous filter through the filtrating that filter obtains, reach transparency, filter the order number and be generally 50～200 orders, operating pressure is 0.2～0.6Mpa;
(5) the transparent feed liquid that step (3) is obtained is sent into electrodialysis plant and is removed inorganic salt, and the feed liquid electrical conductivity behind the desalination should＜50 μ s/cm
2
(6) will distill concentrated, recovery solvent through the transparent filtrating that step (5) obtain, the distillating recovering solvent temperature remains on 100 °～120 ℃, and distillation time is 20～60min;
(7) the transparent feed liquid that step (6) distillating recovering solvent handles be will pass through and 135 ℃ of high-temperature instantaneous sterilization devices, sterilizing time 5～7S pumped into; Pumping into steam pressure again is that 180Kpa, vacuum are that 90Kpa, temperature are 55 ℃ enrichment facility; Concentrated solution concentration reaches at 10%～20% o'clock; Getting into the bottled purity that obtains is 0.01%～0.5% the female graceful liquid Semen sojae atricolor composite extract of Jie girl; Or be that 10%～20% concentrated solution pumps in the press spray tower with concentration; Inlet temperature is 160 °～180 ℃, and outlet temperature is 60 °～80 ℃, and the gained powder product is the Semen sojae atricolor composite extract that contains the female graceful content of low-purity Semen sojae atricolor Jie girl of the female graceful amount 0.1%～5.0% of Jie girl.
2. the female graceful production method of Semen sojae atricolor Jie girl, its characteristic may further comprise the steps:
(1) is raw material with bean cake or soybean seed or soybean cake, is ground into 20～100 purpose powder;
(2) powder that step (1) is obtained is packed in the lixiviate jar of belt stirrer; In the lixiviate jar, add concentration and be the hot water of 20%～80% natural gas level methanol or ethanol or methanol and alcohol mixeding liquid or acetone or ethyl acetate or 80 °～100 ℃; The adding quantity of solvent is 7～12 times of grain weight amount; Fully stir, mixing speed is 60～80r/min, mixing time 6～12h;
(3) will pass through the mixed liquor that step (2) obtains and filter through 50～200 order filters, the material conveying pump power is 10KW, and operating pressure is 0.4～0.6Mpa;
(4) step (3) is carried out microporous filter through the filtrating that filter obtains, reach transparency, filter the order number and be generally 50～200 orders, operating pressure is 0.2～0.6Mpa;
(5) with the transparent filtered solution after the distillation of step (4) gained, through electrodialysis desalination, the feed liquid electrical conductivity behind the desalination must not surpass 50 μ s/cm
2
(6) will pass through macroporous adsorbent resin through the feed liquid behind step (5) desalination; Contained female graceful, the soybean saponin of Jie girl in the material of resin absorption; Effusive feed liquid is a soybean oligo saccharide; After absorption with macroporous adsorbent resin is saturated, molten from eluting with the hot water of 20%～80% natural gas level methanol or ethanol or methanol and alcohol mixeding liquid or acetone or ethyl acetate or 80 °～100 ℃;
(7) eluent that step (6) is obtained is regained solvent through distilling to concentrate;
(8) will be through the spissated female graceful concentrated solution of Jie girl, thin up to the concentration 2%～10% of containing of the distillation of step (7);
(9) can water-soluble, the female graceful biological characteristics that is insoluble to normal-temperature water of Jie girl according to saponin, the solution that step (8) is obtained adopts the high speed separator that is higher than 10000 rev/mins to separate to such an extent that contain female graceful solid formation of Jie girl and the supernatant that contains saponin; Contain the female graceful solid phase Semen sojae atricolor composite extract of Jie girl by (8), (9) step repeated treatments 2～5 times, different according to number of processes, can obtain containing the female graceful liquid Semen sojae atricolor composite extract of 5%～95% Jie girl by butt;
(10) step (9) gained being contained female graceful solid phase Semen sojae atricolor composite extract thin up to the solid content of Jie girl is about 10%; Through sterilization, spray drying; Obtain containing 5%～95% the female graceful powdery soybean composite extract of Jie girl, product purity can be different because of the separation number of times of step (9).
3. the female graceful application in preparation type estrogenic or health food of the Semen sojae atricolor Jie girl of the estrogenic composition of type of having.
Semen sojae atricolor Jie girl femalely gracefully reconcile climacteric syndrome, improve women's secondary sexual characteristics in preparation, the atrophy of control elderly woman height, the health food of senile dementia or the application in the medicine; Its prescription characteristic is: the female graceful dosage of everyone every day of oral pure Jie girl is at＞6.0mg; Between＜the 50mg, can obtain obvious type of estrogen effect.
5. Semen sojae atricolor Jie girl is female gracefully prevents and treats the application in women's cancer drug in preparation; Its prescription characteristic is: by 60 kg body weight adult females; Amplify calculating according to animal test results, take in Jie girl female graceful (G) amount everyone every day and can reach 61.3%, 46.3%, 36.0% respectively for the 250mg/ day for human beings, the 150mg/ day for human beings, 100mg/ day for human beings tumour inhibiting rate.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105594949A (en) * | 2015-12-21 | 2016-05-25 | 长春大学 | Chocolate supplemented with natural botanical xenoestrogen and preparation method thereof |
| US10543243B2 (en) | 2016-06-06 | 2020-01-28 | Charsire Biotechnology Corp. | Soybeam seed extract, method for producing the same and uses thereof |
| CN110960570A (en) * | 2019-12-02 | 2020-04-07 | 长春大学 | Method for producing soyasaponin hypolipidemic granules and hypolipidemic beer products |
| CN114878725A (en) * | 2021-06-22 | 2022-08-09 | 长春大学 | Method for detecting sulfur dioxide in food |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349987A (en) * | 2000-10-20 | 2002-05-22 | 中国食品发酵工业研究所 | Soybean isoflavone and its prepn process with macroporous adsorption resin |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1349987A (en) * | 2000-10-20 | 2002-05-22 | 中国食品发酵工业研究所 | Soybean isoflavone and its prepn process with macroporous adsorption resin |
Non-Patent Citations (1)
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| 曲妍妍等: "染料木黄酮生物活性及抗病机理的研究进展", 《食品工业科技》, no. 08, 31 August 2010 (2010-08-31) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105594949A (en) * | 2015-12-21 | 2016-05-25 | 长春大学 | Chocolate supplemented with natural botanical xenoestrogen and preparation method thereof |
| US10543243B2 (en) | 2016-06-06 | 2020-01-28 | Charsire Biotechnology Corp. | Soybeam seed extract, method for producing the same and uses thereof |
| CN110960570A (en) * | 2019-12-02 | 2020-04-07 | 长春大学 | Method for producing soyasaponin hypolipidemic granules and hypolipidemic beer products |
| CN114878725A (en) * | 2021-06-22 | 2022-08-09 | 长春大学 | Method for detecting sulfur dioxide in food |
| CN114878725B (en) * | 2021-06-22 | 2023-08-11 | 长春大学 | Method for detecting sulfur dioxide in food |
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