Summary of the invention
The object of the invention is to provide a kind of rapid-action, good effect, bioavailability is high, clinical application amount the is few loose normal temperature preparation method of superfine powder three son.
Another object of the present invention is to provide a kind of special bilateral airflow sieving machine that is applied to the loose normal temperature preparation method of superfine powder three son.
The loose normal temperature preparation method of superfine powder three son of the present invention, comprises the steps:
The first step, choose crude drug Fructus Chebulae, Fructus Gardeniae, Fructus Toosendan, through natural drying or dry to moisture≤10%;
Second step, at 20 ℃~25 ℃ temperature, above-mentioned raw materials medicine is pulverized respectively, obtained granularity 60~80 object Chinese medicine coarse powder;
The 3rd step, by above-mentioned Chinese medicine coarse powder according to the weight ratio mix homogeneously of 1:1:1;
The 4th step, the 3rd step gained coarse powder is placed in rod mill and is pulverized after, by wind, be fed into special bilateral airflow sieving machine and cross 500 mesh sieves, sieving obtains air-flow that the powder of particle diameter≤25 micron produces by air-introduced machine and is transported to and in tornado feeder set, collects and obtain the loose finished product of superfine powder three son; The powder of not crossing 500 mesh sieves is transported to circulation pulverizing again in described rod mill through collection funnel and by pipeline.Loose being sub-packed in aluminium foil bag of finished product superfine powder three sons, every bag weighs 100g, 200g, 500g, 1000g, 2000g etc.
Special bilateral airflow sieving machine of the present invention, comprises casing and the downward screen drum of chamber intermediate openings that sets within it, order number >=500 order of described screen drum; The nozzle of screen drum is connected mutually with the funnel upper shed that is arranged on casing bottom; Chassis cavity is connected with air-introduced machine by the negative pressure chamber on top; At the region division between screen drum and casing, there is the door font rotation spray trachea being formed by transverse tube and standpipe, the puff prot of described door font rotation spray trachea is for to be axially opened in the groove on its tube wall along described transverse tube and standpipe respectively, described groove is offered towards screen drum direction respectively, the groove width 0.5-2 millimeter of groove, the middle part of transverse tube and drive motors driving coupling; In screen drum centre position, be provided with blast pipe, the air outlet of described blast pipe is connected and is rolled and be connected by rolling bearing with the transverse tube middle part of door font rotation spray trachea through screen drum; The air inlet of blast pipe extends outside described hopper side walls and is connected with the blast orifice of aerator; In screen drum, be provided with feed pipe, be provided with buffering umbrella above the discharging opening of described feed pipe, the charging aperture of feed pipe extends outside hopper side walls and is communicated with powder conveying fan air outlet through wind stopping device.
The air inducing of described air-introduced machine is pressed as 1000pa, and air inducing amount is 3000m
3/ h; The rotating speed of described drive motors is 155 revs/min; The blast pressure of described aerator is 33kpa, and the blow rate required is 320m
3/ h; The wind pressure that goes out of described powder conveying fan is 24kpa, and air output is 150m
3/ h; The sidewall of described screen drum and roof respectively with corresponding standpipe and transverse tube between distance be 25 millimeters.
The invention has the advantages that whole preparation process all carries out at normal temperatures, do not need low temperature or special additional conditions; Final drug particle diameter≤25 micron of preparation, cell wall breaking rate (sporoderm-broken rate reaches more than 95%) and bioavailability have greatly been improved, strengthened pharmacological action, thereby reduced treatment disease dosage, saved medical material, also abnormal smells from the patient and the mouthfeel of medicine have been improved simultaneously, make directly to adopt water way administration to be achieved, changed the deficiency that Chinese powder medicine can only spice can not be drunk water and takes, three of superfine powder are loose simultaneously can evenly stick on animal pellet feed, has solved particulate material and can not mix with Chinese medicine the problem of using; The bidirectional airflow sieving machine using in the inventive method, has simplified the loose preparation technology of superfine powder three son greatly, has realized the object that Chinese powder medicine quality standard is controlled, be suitable for large-scale industrial production.
The clinical therapeutic efficacy of preparation of the present invention is verified by following test:
The sub-powder ultramicro powder extracorporeal bacteria inhibitor test of test examples 1 three
1. the required experiment material of bacteriostatic experiment
1.1 strains (medical science antibacterial):
Staphylococcus albus, thin staphylococcus aureus, 2 type Hemolytic Type streptococcus, bacillus pyocyaneus, escherichia coli, Bacillus proteus, the reference culture that experiment provides for Microbiological Lab of Agricultural University Of He'nan with antibacterial.
1.2 culture medium:
Select Sharpe proof agar base, be generally called the weak culture medium in husky fort, its composition is glucose 409, peptone 109, agar 159, distilled water 1000ml.After above-mentioned mixing, add 200mg chloromycetin, 250mg cycloheximide, 121 ℃, standby after sterilizing in 20 minutes.
Beta hemolytic streptococcus is selected blood agar culture-medium, a little less than husky fort, in culture medium, adds fresh Sanguis caprae seu ovis 5% to make.
1.3 medicines:
Three sub-powder ultramicro powders, employing the present invention preparation.
Three sons are loose: according to second of < < Chinese veterinary pharmacopoeia > > version in 2010, at laboratory, prepare.
Benzylpenicillin sun mark, the place of production is Hangzhou Tian He microorganism reagent company limited, lot number is 20060208, is numbered C023;
Chloromycetin sun mark, the place of production is Hangzhou Tian He microorganism reagent company limited, lot number is 20060208, is numbered C023.
2. anti-bacteria experimental technique
2.1 test groupings:
Be divided into that three sub-powder ultramicro powders, three sons are loose, chloromycetin, penicillin and five groups of blank, every kind of bacterium is divided into 3 samples
2.2 bacterial vaccine inoculation methods:
Fresh antibacterial liquid is drawn to be respectively coated with by swab stick and plant in the plate containing culture medium a little less than husky fort.
2.3 cup-plate methods:
Adopt the weak culture medium in husky fort, each plate is placed two sterilizing stainless steel tubules that diameter is 5mm, the three sub-powder ultramicro powders and the loose medicinal liquid of three sons and the blank liquid (normal saline) that in pipe, add respectively 250 μ l (0.675mg), in plate, put into respectively benzylpenicillin (10 μ g) and chloromycetin (30 μ g) standard drug sensitive test paper, be placed in 37 ℃ of constant incubators, within 14 hours, observe the effect of medicine to antibacterial.
2.4 anti-bacteria experimental results: observed result after 24 hours, vernier caliper measurement inhibition zone diameter.
2.4 statistical analysis
Data acquisition carries out statistical analysis with SPSSl3.0 software kit, between a plurality of rates of enumeration data, relatively uses X
2check, measurement data with
± S represents, a plurality of sample averages are relatively used variance analysis.
3. anti-bacteria experimental result
3.1 3 sub-powder ultramicro powders, three sons are loose and the antibacterial outcome record of chloromycetin in Table 1.
The sub-powder ultramicro powder of table 1 three, three sons are loose and the measurement result (mm) of chloromycetin inhibition zone diameter (
± S)
△ P<0.05, has significant difference
As seen from Table 1, three sub-powder ultramicro powder groups and blank group compare gram negative bacilli (escherichia coli, Bacillus proteus and bacillus pyocyaneus) inhibitory action significant difference (P<0.05), illustrates that three sub-powder ultramicro powders have bacteriostasis; Three sub-powder ultramicro powder groups and the loose group of three sons have significant difference (P<O.05) to above-mentioned bacillus inhibitory action, according to antibacterial ring size and the relation that increases progressively of bacteriostasis, illustrate that it is better than the loose group of three sons; Three sub-powder ultramicro powder groups and chloromycetin group have significant difference (P<O.05) to above-mentioned bacillus inhibitory action, according to antibacterial ring size, illustrate that with the relation that increases progressively of bacteriostasis it is better than chloromycetin group.
3.2. three sub-powder ultramicro powders, three sons are loose and the antibacterial outcome record of penicillin in Table 2
The sub-powder ultramicro powder of table 2 three, three sons are loose and the measurement result (mm) of blue or green plain inhibition zone diameter (
± S)
△ P<0.05, has significant difference
As seen from Table 2, three sub-powder ultramicro powder groups and blank group compare gram-positive cocci (staphylococcus aureus, Staphylococcus albus and beta hemolytic streptococcus) inhibitory action significant difference (P<O.05), illustrates that three sub-powder ultramicro powders have bacteriostasis; Three sub-powder ultramicro powder groups and penicillin group have significant difference (P<O.05) to above-mentioned coccus inhibitory action, according to antibacterial ring size and the relation explanation penicillin group bacteriostasis that increases progressively of bacteriostasis, be better than three sub-powder ultramicro powder groups, and three sub-powder ultramicro powder group group bacteriostasis are better than the loose group of three sons.
Application test in the sub-powder ultramicro powder body of experiment embodiment 2, three
1. required experiment material is tested in treatment
1.1 strain medical science antibacterials:
2 type Hemolytic streptococcuss, escherichia coli and Bacillus proteus, the reference culture that experiment provides for Microbiological Lab of Henan Medical Univ. with antibacterial.
1.2 animals: 30 long white growing and fattening pigs, 83 ± 3 days
1.3 medicines:
Three sub-powder ultramicro powders, employing the present invention preparation.
Three sons are loose: according to second of < < Chinese veterinary pharmacopoeia > > version in 2010, at laboratory, prepare.
2. experimental technique
2.1 test groupings:
Drug therapy is divided into three sub-powder ultramicro powder 15g/ head/skies, the loose fine powder 30g/ of three sons head/sky, three groups of blank, and every group is 10.
Antibacterial infects and is divided into escherichia coli and 2 type Hemolytic streptococcuss
2.2 infection methods
The escherichia coli of laboratory cultures and 2 type Hemolytic streptococcuss, after colony counting, become every milliliter approximately 1.0 * 10 with normal saline dilution respectively
9the concentration of individual thalline, piglet lumbar injection, dosage is 2mL/ head.
2.3 result of the tests are processed
The piglet of bacterial infections, is defined as escherichia coli and 2 type Hemolytic streptococcus classical symptoms through breeding observing and dissection, and the Drug therapy that rear use is different, records therapeutic outcome.
3. result of the test (result of the test is in Table 3)
The sub-powder ultramicro powder of table 3 three, the loose treatment of three sons piglet antibacterial infection experiment result
As can be seen from Table 3: three sub-powder ultramicro powder groups and blank group compare known, three sub-powder ultramicro powders are the piglet successful by 2 type Hemolytic streptococcuss and coli-infection to treatment, illustrates that three sub-powder ultramicro powders have bacteriostasis; Three sub-powder ultramicro powder group group therapeutical effect are obviously better than the loose group of three sons.
Accompanying drawing explanation
Fig. 1 is the production equipment system figure of the method for the invention.
Fig. 2 is the structural representation of special bilateral airflow sieving machine of the present invention.
The specific embodiment
As shown in Figure 1, 2, the loose normal temperature preparation method of superfine powder three son of the present invention, comprises the steps:
The first step, choose crude drug Fructus Chebulae, Fructus Gardeniae, Fructus Toosendan, through natural drying or dry to moisture≤10%;
Second step, at 20 ℃~25 ℃ temperature, above-mentioned raw materials medicine is pulverized respectively, obtained granularity 60~80 object Chinese medicine coarse powder;
The 3rd step, by above-mentioned raw materials medicine coarse powder according to the weight ratio mix homogeneously of 1:1:1;
The 4th step, first open powder conveying fan 15, air-introduced machine 5, aerator 11, drive motors 8.The blow pressure of powder conveying fan 15 is 24kpa, and blowing amount is 150m
3/ h; The air inducing of air-introduced machine 5 is pressed as 1000pa, and air inducing amount is 3000m
3/ h; The blast pressure of aerator 11 is 33kpa, blow rate required 320m
3/ h; The rotating speed of drive motors 8 is 155 revs/min;
1, the 3rd step gained coarse powder is placed in after the interior pulverizing of rod mill 16, the interior powder body to be fractionated of rod mill 16 is brought into by high velocity air and is arranged in the downward screen drum 2 of casing 1 inner chamber intermediate openings through wind stopping device 14, feed pipe 12 under the power of the blowing effect of powder conveying fan 15, and reduce the impulsive force of powder body to be fractionated in screen drum 2 by buffering umbrella 13, make powder body to be fractionated in the interior dispersion of screen drum 2; The order number of screen drum 2 is 500 orders;
2, the powder body to be fractionated of screen drum 2 interior dispersions carries out classification by screen drum 2 under air-introduced machine 5 effects, through the fine powder of screen drum 2, collects and obtain the loose finished product of superfine powder three son under the negative-pressure air-flow effect of air-introduced machine 5 generations in the negative pressure chamber 4 on casing 1 top is transported to tornado feeder set 17.The funnel 3 that powder body by screen drum 2 does not sink through being connected mutually with screen drum 2 under sheds is collected and is transported in described rod mill 16 circulation again by pipeline 18 and pulverizes;
3, door font rotation spray trachea rotates by rolling bearing 10 under drive motors 8 drives, the wind that aerator 11 is sent is in blast pipe 9 enters described transverse tube 6 and standpipe 7, and through be opened in vertically respectively the puff prot ejection on transverse tube 6 and standpipe 7 tube walls with groove structure towards screen drum 2 directions, at screen drum 2 end faces and side, form even blowback air-flow in screen drum 2, make the further atomization of powder body in screen drum 2, stop the powder body blocking screen mesh that lumps simultaneously on screen drum 2, make not powder body by screen drum 2 along with the air-flow whirlpool forming sinks to entering in funnel 3.The groove width that is opened in vertically respectively groove on transverse tube 6 and standpipe 7 tube walls is selected as required between 0.5 millimeter-2 millimeters, and between the roof of transverse tube 6 and standpipe 7 and corresponding screen drum 2 and sidewall, distance is respectively 25 millimeters.
The loose finished product of the superfine powder of preparation three son is sub-packed in aluminium foil bag and is stored, and every bag weighs 500g or 1000g.